Publications by authors named "Zisen Liu"

11 Publications

  • Page 1 of 1

Urinary metabolomic analysis to identify potential markers for the diagnosis of tuberculosis and latent tuberculosis.

Arch Biochem Biophys 2021 Jun 14;704:108876. Epub 2021 Apr 14.

NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China. Electronic address:

Tuberculosis (TB) is a serious infectious disease with high infection and mortality rates. 5%-10% of the latent tuberculosis infections (LTBI) are likely to develop into active TB, and there are currently no clinical biomarkers that can distinguish between LTBI, active TB and other non-tuberculosis populations. Therefore, it is necessary to develop rapid diagnostic methods for active TB and LTBI. In this study, urinary metabolome of 30 active TB samples and the same number of LTBI and non-TB control samples were identified and analyzed by UPLC-Q Exactive MS. In total, 3744 metabolite components were obtained in ESI- mode and 4086 in ESI + mode. Orthogonal partial least square discriminant analysis (OPLS-DA) and hierarchical cluster analysis (HCA) showed that there were significant differences among LTBI, active TB and non-TB. Six differential metabolites were screened in positive and negative mode, 3-hexenoic acid, glutathione (GSH), glycochenodeoxycholate-3-sulfate, N-[4'-hydroxy-(E)-cinnamoyl]-l-aspartic acid, deoxyribose 5-phosphate and histamine. The overlapping pathways differential metabolites involved were mainly related to immune regulation and urea cycle. The results showed that the urine metabolism of TB patients was disordered and many metabolic pathways changed. Multivariate statistical analysis revealed that GSH and histamine were selected as potential molecular markers, with area under curve of receiver operating characteristic curve over 0.75. Among the multiple differential metabolites, GSH and histamine changed to varying degrees in active TB, LTBI and the non-TB control group. The levels of GSH and histamine in 48 urinary samples were measured by ELISA in validation phase, and the result in our study provided the potential for non-invasive biomarkers of TB.
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http://dx.doi.org/10.1016/j.abb.2021.108876DOI Listing
June 2021

Effects of bentonite on the growth process of submerged macrophytes and sediment microenvironment.

J Environ Manage 2021 Jun 8;287:112308. Epub 2021 Mar 8.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

The effects of clay mineral bentonite on the growth process of submerged macrophyte V. spiralis and sediment microenvironment were investigated in the study for the first time, aiming to determine whether it is suitable for application in the field of ecological restoration. The growth index, and physiological and biochemical index of V. spiralis in the experiments were measured once a month, and the changes of rhizosphere microorganisms and physicochemical properties of sediments were also studied at the same time. The results demonstrated that bentonite can effectively promote the growth of V. spiralis. The treatment groups of RB1/1 and MB1/5 (the mass ratios of bentonite to sediment were 1/1 and 1/5, respectively.) showed the best V. spiralis growth promotion rates which were 18.78%, and 11.79%, respectively. The highest microbial diversity and abundance existed in group of RB10 (the mass ratio of sediment to bentonite was 10/1), in which the OTUs, Shannon, Chao and Ace were 1521.0, 5.20, 1712.26, and 1686.31, respectively. Bentonite was conducive to the propagation of rhizosphere microorganisms, and further changed the physical and chemical properties of the sediment microenvironment. The nutrient elements dissolved from bentonite may be one of the main reasons that promoted the growth of V. spiralis. The purpose of this result is to prove that bentonite can be further applied as sediment improver and growing media in ecological restoration projects in eutrophic shallow lakes.
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http://dx.doi.org/10.1016/j.jenvman.2021.112308DOI Listing
June 2021

Spatial and seasonal variation of water parameters, sediment properties, and submerged macrophytes after ecological restoration in a long-term (6 year) study in Hangzhou west lake in China: Submerged macrophyte distribution influenced by environmental variables.

Water Res 2020 Nov 2;186:116379. Epub 2020 Sep 2.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; School of Environmental Studies, China University of Geosciences, Wuhan 430074, China. Electronic address:

Submerged macrophyte restoration is the key stage in the reestablishment of an aquatic ecosystem. Previous studies have paid considerable attention to the effect of multiple environmental factors on submerged macrophytes. Meanwhile, few studies have been conducted regarding the spatial and seasonal characteristics of water and sediment properties and their long-term relationship with submerged macrophytes after the implementation of the submerged macrophytes restoration project. On a monthly basis, we monitored the spatial and seasonal variation in water parameters, sediment properties, and the submerged macrophyte characteristics of West Lake in Hangzhou from August 2013 to July 2019. From these measurements, we characterized the relationship between environmental factors and submerged macrophytes. Water nutrient concentrations continuously decreased with time, and the accumulation of sediment nutrients was accelerated as the submerged macrophyte communities developed on a long-term scale. The results indicated that the difference in water parameters was due to seasonal changes and land-use types in the watershed. The differences in the sediment properties were mainly attributed to seasonal changes and changes in the flow field. Redundancy analysis showed that the influence of water nutrients on the submerged macrophyte distribution was greater than that of sediment nutrients. The result also suggested that the developed root system, high stoichiometric homeostasis coefficients of P, and compensation ability of substantial leaf tissue may lead to a large distribution of Vallisneria natans in West Lake in Hangzhou. The correlation of water parameters and sediment properties with submerged macrophytes for a long time was very important as the restoration was achieved. To ensure the stability of the aquatic ecosystem after performing the submerged macrophyte restoration, a greater emphasis must be placed on reestablishing the entire ecosystem, including the restoration of aquatic animals and fish stocks. We expect these findings to serve as a reference for researchers and government agencies in the field of aquatic ecosystem restoration.
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http://dx.doi.org/10.1016/j.watres.2020.116379DOI Listing
November 2020

The promotion effects of silicate mineral maifanite on the growth of submerged macrophytes Hydrilla verticillata.

Environ Pollut 2020 Dec 23;267:115380. Epub 2020 Aug 23.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China. Electronic address:

The effects of maifanite on the physiological and phytochemical process of submerged macrophytes Hydrilla verticillate (H.verticillata) were investigated for the first time in the study. The growth index: plant biomass, root length, plant height and leaf spacing, and physiological and phytochemical indexes: chlorophyll, soluble protein, malondialdehyde (MDA), peroxidase (POD), superoxide dismutase (SOD) content and vitality of the roots of H.verticillata were tested. The results found that maifanite can significantly promote the growth of H.verticillata. The modified maifanite were more conducive to plant growth compared with the raw maifanite, and the MM1 group had the best growth promoting effect. The physiological and phytochemical indexes showed that maifanite can delay the aging process of H.verticillata (P < 0.05). The possible reasons for promoting H.verticillata growth were that maifanite can provide excellent propagation conditions for plant rhizosphere microorganisms, contains abundant major and microelements, and improve the sediment microenvironment. This study may provide a technique for the further application of maifanite in the field of ecological restoration.
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http://dx.doi.org/10.1016/j.envpol.2020.115380DOI Listing
December 2020

Synergistic control of internal phosphorus loading from eutrophic lake sediment using MMF coupled with submerged macrophytes.

Sci Total Environ 2020 Aug 5;731:138697. Epub 2020 May 5.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

Sediment phosphorus (P) is the main source of endogenous P for lake eutrophication. An in-situ combined technology for determination the removal effect of sediment P in all fractions was first developed using the novel modified maifanite (MMF) and submerged macrophytes in this study. MMF was synthesized using an acidification process (2.5 mol/L HSO) and then a calcination (400 °C) method. The morphology and structure of MMF were characterized by XRD, SEM, XPS, and BET. We tested the removal effects of sediment P by MMF and submerged macrophytes in combination and separately. The results demonstrated that the synergistic removal capacity of sediment P using MMF coupled with submerged macrophytes was higher than the sum of them applied separately. MMF could promote the submerged macrophytes growth and enhance the adsorption of extra P on MMF through root oxygenation and nutrient allocation. The microcosm experiment results showed that sediment from fMMF+V. spiralis exhibited the most microbial diversity and abundance among the sediment. The combination of MMF and submerged macrophytes increased the Firmicutes abundance and decreased the Bacteroidetes. These results indicated that adsorption-biological technology can be regarded as a novel and competitive technology to the endogenous pollution control in eutrophic shallow lakes.
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http://dx.doi.org/10.1016/j.scitotenv.2020.138697DOI Listing
August 2020

Serum level of IL-1ra was associated with the treatment of latent tuberculosis infection in a Chinese population.

BMC Infect Dis 2020 May 8;20(1):330. Epub 2020 May 8.

NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis Research, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 9 Dong Dan San Tiao, Dongcheng District, Beijing, 100730, China.

Background: Dynamically changed levels of serum cytokines might predict the development of active TB from latent tuberculosis infection (LTBI) and monitor preventive treatment effectiveness. The aim of the study was to identify potential serum cytokines associated with LTBI treatment which might predict active disease development in a Chinese population.

Methods: Based on a randomized controlled trial aiming to explore short-course regimens for LTBI treatment, the dynamic changes of serum cytokines determined by bead-based multiplex assays were investigated for the participants who developed active TB during follow-up and age and gender matched controls stayed healthy.

Results: Totally, 21 patients diagnosed with active tuberculosis (TB) during the 2-year follow-up (12 from treated groups and 9 from untreated controls) and 42 age and gender matched healthy controls (24 from treated groups and 18 from untreated controls) were included in the study. Before treatment, serum IL-1ra was statistically higher among those who developed active disease during follow-up as compared with those stayed healthy. As for treated participants, the levels of IL-1ra were significantly lower after treatment in comparison with those before treatment both in active TB group (p = 0.002) and non-TB group (p = 0.009). For untreated participants, the levels of IL-1ra were not statistically different between different time points both in active TB group (p = 0.078) and non-TB group (p = 0.265).

Conclusion: Our results suggested that declined serum level of IL-1ra was associated with LTBI treatment. Further studies are needed to verify whether it could be used to evaluate LTBI treatment and to predict active disease development.
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http://dx.doi.org/10.1186/s12879-020-05047-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206663PMC
May 2020

Effects of maifanite on growth, physiological and phytochemical process of submerged macrophytes Vallisneria spiralis.

Ecotoxicol Environ Saf 2020 Feb 21;189:109941. Epub 2019 Nov 21.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan, 430070, China.

The restoration of submerged plants is critical for the reconstruction of eutrophic lake ecosystems. The growth of submerged plants is influenced by many factors. For the first time in this study, the effects of silicate-mineral maifanite supplement on the growth, physiological and phytochemical process of Vallisneria spiralis (V. spiralis) were investigated by an outdoor PVC barrel experiment, to provide a technical reference for further applications in aquatic ecological restoration. The results show that the maifanite could significantly promote the growth of V. spiralis. Specifically, the biomass, height, number of leaves, leaf width, root length, and root activity of V. spiralis in the maifanite-supplemented group were better than those of the control (P < 0.05). Moreover, the modified maifanite group performed better than the raw maifanite group (P < 0.05). The photosynthetic pigment, root activity, and the malondialdehyde and peroxidase activity of the maifanite-treated V. spiralis were better than those of the control to some extent. It was found that maifanite contained abundant major and trace elements, which are required for the growth of V. spiralis. It is concluded that maifanite is beneficial to the growth of V. spiralis and can be further applied to the ecological restoration of eutrophic lakes.
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http://dx.doi.org/10.1016/j.ecoenv.2019.109941DOI Listing
February 2020

Serial testing of Mycobacterium tuberculosis infection in Chinese village doctors by QuantiFERON-TB Gold Plus, QuantiFERON-TB Gold in-Tube and T-SPOT.TB.

J Infect 2019 04 30;78(4):305-310. Epub 2019 Jan 30.

NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China. Electronic address:

Objectives: To evaluate the performance of QuantiFERON-TB Gold Plus (QFT-Plus) on Mycobacterium tuberculosis (MTB) infection test among registered village doctors from China.

Methods: MTB infection of the registered village doctors in Zhongmu County were tested using QFT-Plus and two other interferon-gamma release assays (IGRAs) in parallel: QuantiFERON-TB Gold In-Tube (QFT) and T-SPOT.TB (T-SPOT). Retests were carried out for baseline positives at 3 and 6 months later, respectively.

Results: A total of 616 village doctors were included in the baseline examination. The positivity of QFT, QFT-Plus and T-SPOT was 27.91% (168/602), 31.22% (187/599) and 27.70% (169/610), respectively. The concordance between QFT and QFT-Plus was 94.81% (Kappa coefficient: 0.87) and between T-SPOT and QFT-Plus was 88.93% (Kappa coefficient: 0.73). Reversions were frequently observed for all three assays. With respect to QFT-Plus, the quantitative results of reversions in the serial testing were mostly distributed in an "uncertain range" zone (0.2-0.7 IU/mL). Similar patterns of distribution were observed for QFT and T-SPOT as well.

Conclusion: Village doctors should gain more attention as an at-risk group for TB infection control in rural China. Our results support, by means of serial testing, a good agreement between QFT-Plus and QFT in Chinese population.
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http://dx.doi.org/10.1016/j.jinf.2019.01.008DOI Listing
April 2019

Short-course regimens of rifapentine plus isoniazid to treat latent tuberculosis infection in older Chinese patients: a randomised controlled study.

Eur Respir J 2018 12 20;52(6). Epub 2018 Dec 20.

MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology and Center for Tuberculosis, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Latent tuberculosis infection (LTBI) management is now a critical component of the World Health Organization's End TB Strategy.In this randomised controlled trial (Chinese Clinical Trial Registry identifier ChiCTR-IOR-15007202), two short-course regimens with rifapentine plus isoniazid (a 3-month once-weekly regimen and a 2-month twice-weekly regimen) were initially designed to be evaluated for rural residents aged 50-69 years with LTBI in China.Due to the increasingly rapid growth and unexpected high frequency of adverse effects, the treatments were terminated early (after 8 weeks for the once-weekly regimen and after 6 weeks for the twice-weekly regimen). In the modified intention-to-treat analysis on the completed doses, the cumulative rate of active disease during 2 years of follow-up was 1.21% (14 out of 1155) in the untreated controls, 0.78% (10 out of 1284) in the group that received the 8-week once-weekly regimen and 0.46% (six out of 1299) in the group that received the 6-week twice-weekly regimen. The risk of active disease was decreased, with an adjusted hazard ratio of 0.63 (95% CI 0.27-1.43) and 0.41 (95% CI 0.15-1.09) for the treatments, respectively. No significant difference was found in the occurrence of hepatotoxicity (1.02% (13 out of 1279) 1.17% (15 out of 1279); p=0.704).The short regimens tested must be used with caution among the elderly because of the high rates of adverse effects. Further work is necessary to test the ultrashort regimens in younger people with LTBI.
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http://dx.doi.org/10.1183/13993003.01470-2018DOI Listing
December 2018

Investigation on the adsorption of phosphorus in all fractions from sediment by modified maifanite.

Sci Rep 2018 10 23;8(1):15619. Epub 2018 Oct 23.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.

Sediment phosphorus (P) removal is crucial for the control of eutrophication, and the in-situ adsorption is an essential technique. In this study, modified maifanite (MMF) prepared by acidification, alkalization, salinization, calcination and combined modifications, respectively, were first applied to treat sediment P. The morphology and microstructure of MMF samples were characterized by X-ray fluorescence (XRF), Fourier transform infrared (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and Brunauer-Emmett-Teller (BET). Various adsorption parameters were tested, such as dosage of maifanite, time, operation pH and temperature. The adsorption mechanisms were also investigated and discussed. Results showed that CMMF-H2.5-400 (2.5 mol/L HSO and calcined at 400 °C) exhibited the highest P adsorption capacity. Thus, it was selected as the in-situ adsorbent material to control the internal P loading. Under the optimal conditions of dynamic experiments, the adsorption rates of TP, IP, OP, Fe/Al-P and Ca-P by CMMF-H2.5-400 were 37.22%, 44.41%, 25.54%, 26.09% and 60.34%, respectively. The adsorption mechanisms analysis revealed that the adsorption of P onto CMMF-H2.5-400 mainly by ligand exchange. Results of this work indicated that the modification treatment could improve the adsorption capacity of maifanite, and CMMF-H2.5-400 could be further applied to eutrophication treatment.
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http://dx.doi.org/10.1038/s41598-018-34144-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6199331PMC
October 2018

Synergistic removal effect of P in sediment of all fractions by combining the modified bentonite granules and submerged macrophyte.

Sci Total Environ 2018 Jun 19;626:458-467. Epub 2018 Feb 19.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

The removal efficiency of sediment phosphorus (P) with the in-situ synergistic effect of modified bentonite granules (MBG) and Vallisneria spiralis (V. spiralis) in West Lake, Hangzhou, China was investigated for the first time in the study. CMBG-Na10-450 (nitrification (10% NaCO)-calcination (450 °C) combined modification) was prepared and characterized, and the removal effects of sediment P of all fractions with CMBG-Na10-450 and V. spiralis in combination and separately were evaluated in batch experiments. Results showed that CMBG-Na10-450 could promote the growth of V. spiralis, and the residual P of the sediment not adsorbed on CMBG-Na10-450 was changed through root oxygenation and nutrition allocation, and then enhanced the extra P adsorption on CMBG-Na10-450. The combination of MBG and V. spiralis exhibited a synergistic removal effect higher than the summation of MBG and V. spiralis applied separately. The results of microcosm experiments showed that the combination of CMBG-Na10-450 and V. spiralis enhanced the function of P metabolism by increasing the special genus that belongs to the family Erysipelotrichaceae.
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http://dx.doi.org/10.1016/j.scitotenv.2018.01.093DOI Listing
June 2018