Publications by authors named "Zili Zhou"

26 Publications

  • Page 1 of 1

Effects of Physical Stimulation in the Field of Oral Health.

Scanning 2021 7;2021:5517567. Epub 2021 Apr 7.

Stomatological Materials Laboratory, School of Stomatology, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, Shandong 271016, China.

Physical stimulation has been widely used in clinical medicine and healthcare due to its noninvasiveness. The main applications of physical stimulation in the oral cavity include laser, ultrasound, magnetic field, and vibration, which have photothermal, cavitation, magnetocaloric, and mechanical effects, respectively. In addition, the above four stimulations with their unique biological effects, which can play a role at the gene, protein, and cell levels, can provide new methods for the treatment and prevention of common oral diseases. These four physical stimulations have been used as important auxiliary treatment methods in the field of orthodontics, implants, periodontal, dental pulp, maxillofacial surgery, and oral mucosa. This paper systematically describes the application of physical stimulation as a therapeutic method in the field of stomatology to provide guidance for clinicians. In addition, some applications of physical stimulation in specific directions are still at the research stage, and the specific mechanism has not been fully elucidated. To encourage further research on the oral applications of physical stimulation, we elaborate the research results and development history of various physical stimuli in the field of oral health.
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http://dx.doi.org/10.1155/2021/5517567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049832PMC
April 2021

Long non-coding RNA TUG1 promotes airway remodeling and mucus production in asthmatic mice through the microRNA-181b/HMGB1 axis.

Int Immunopharmacol 2021 May 26;94:107488. Epub 2021 Feb 26.

Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, PR China. Electronic address:

MicroRNA-181b (miR-181b) has been well noted with anti-inflammatory properties in several pathological conditions. It has also been suggested to be downregulated in patients with asthma. In this study, we explored the function of miR-181b in airway remodeling in asthmatic mice and the molecular mechanism. A mouse model with asthma was induced by ovalbumin (OVA) challenge, and miR-181b was found to be downregulated in lung tissues in the OVA-challenged mice. Overexpression of miR-181b was introduced in mice, after which the respiratory resistance, inflammatory infiltration, mucus production, and epithelial-mesenchymal transition (EMT) and fibrosis in mouse airway tissues were decreased. The integrated bioinformatics analysis suggested long non-coding RNA (lncRNA) TUG1 as a sponge for miR-181b. miR-181 directly targeted high mobility group box 1 (HMGB1) mRNA. HMGB1 was suggested to enhance activation of the nuclear factor kappa B (NF-κB) signaling. Further upregulation of lncRNA TUG1 blocked the protective functions of miR-181b in asthmatic mice. To conclude, this study evidenced that lncRNA TUG1 reinforces HMGB1 expression through sequestering microRNA-181b, which activates the NF-κB signaling pathway and promotes airway remodeling in asthmatic mice. This study may provide novel ideas in asthma management.
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http://dx.doi.org/10.1016/j.intimp.2021.107488DOI Listing
May 2021

PLAGL2 promotes the proliferation and migration of gastric cancer cells via USP37-mediated deubiquitination of Snail1.

Theranostics 2021 1;11(2):700-714. Epub 2021 Jan 1.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Road No. 1277, Wuhan 430022, China.

PLAGL2 (pleomorphic adenoma gene like-2), a zinc finger PLAG transcription factor, is aberrantly expressed in several malignant tumors. However, the biological roles of PLAGL2 and its underlying mechanism in gastric cancer (GC) remain unclear. A series of experiments and were conducted to reveal the role of PLAGL2 in GC progression. The data revealed that PLAGL2 promotes GC cell proliferation, migration, invasion, and EMT and . Mechanistically, we demonstrated the critical role of PLAGL2 in the stabilization of snail family transcriptional repressor 1 (Snail1) and promoting Snail1-mediated proliferation and migration of GC cells. PLAGL2 activated the transcription of deubiquitinase USP37, which then interacted with and deubiquitinated Snail1 protein directly. In addition, GSK-3β-dependent phosphorylation of Snail1 protein is essential for USP37-mediated Snail1 deubiquitination regulation. In general, PLAGL2 promotes the proliferation and migration of GC cells through USP37-mediated deubiquitination of Snail1 protein. This work provided potential therapeutic targets for GC treatment.
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http://dx.doi.org/10.7150/thno.47800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738862PMC
January 2021

TM4SF1 promotes EMT and cancer stemness via the Wnt/β-catenin/SOX2 pathway in colorectal cancer.

J Exp Clin Cancer Res 2020 Nov 5;39(1):232. Epub 2020 Nov 5.

Department of General Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China.

Background: Transmembrane 4 L six family member 1 (TM4SF1) is upregulated in several epithelial cancers and is closely associated with poor prognosis. However, the role of TM4SF1 and its potential mechanism in colorectal cancer (CRC) remain elusive.

Methods: We investigated the expression of TM4SF1 in the Oncomine, the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases and confirmed the results by immunohistochemistry (IHC), qPCR and Western blotting (WB) of CRC tissues. The effect of TM4SF1 on the epithelial-to-mesenchymal transition (EMT) and cancer stemness of CRC cells was investigated by Transwell, wound healing and sphere formation assays. A series of in vitro and in vivo experiments were conducted to reveal the mechanisms by which TM4SF1 modulates EMT and cancer stemness in CRC.

Results: TM4SF1 expression was markedly higher in CRC tissues than in non-tumour tissues and was positively correlated with poor prognosis. Downregulation of TM4SF1 inhibited the migration, invasion and tumour sphere formation of SW480 and LoVo cells. Conversely, TM4SF1 overexpression significantly enhanced the migration, invasion and tumoursphere formation potential of CRC cells, Additionally, TM4SF1 silencing inhibited the EMT mediated by transforming growth factor-β1 (TGF-β1). Mechanistically, gene set enrichment analysis (GSEA) predicted that the Wnt signalling pathway was one of the most impaired pathways in TM4SF1-deficient CRC cells compared to controls. The results were further validated by WB, which revealed that TM4SF1 modulated SOX2 expression in a Wnt/β-catenin activation-dependent manner. Furthermore, we found that knockdown of TM4SF1 suppressed the expression of c-Myc, leading to decreased c-Myc binding to the SOX2 gene promoter. Finally, depletion of TM4SF1 inhibited metastasis and tumour growth in a xenograft mouse model.

Conclusion: Our study substantiates a novel mechanism by which TM4SF1 maintains cancer cell stemness and EMT via the Wnt/β-catenin/c-Myc/SOX2 axis during the recurrence and metastasis of CRC.
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http://dx.doi.org/10.1186/s13046-020-01690-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643364PMC
November 2020

Development and validation of MMR prediction model based on simplified clinicopathological features and serum tumour markers.

EBioMedicine 2020 Nov 20;61:103060. Epub 2020 Oct 20.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Road No. 1277, Wuhan, Hubei 430022, China. Electronic address:

Background: Although simplified clinicopathological features and serum tumour markers (STMs) were reported to be associated with the status of mismatch repair (MMR) in colorectal cancer (CRC) patients, their predictive value alone or in combination for MMR status remains unknown.

Methods: A retrospective analysis of 3274 participants with MMR testing and STMs measurements from two institutions was conducted. The prediction model was developed in the primary cohort that consisted of 1964 participants. Best subset regression was applied to select the most useful predictors from the primary dataset. The performance of the nomogram was evaluated with respect to its calibration, discrimination, and clinical usefulness. External validation was performed in an independent validation cohort of 1310 consecutive CRC patients.

Findings: Among the ten simplified clinicopathological features, seven variables were selected as the best subset of risk factors to develop pathology-based model, including age, tumour diameters, histology, tumour location, perineural invasion, the number of sampled lymph nodes (LNs) and positive LNs. The model showed good calibration and discrimination, with an AUC of 0.756 (95% CI, 0.722 to 0.789) in the primary cohort and 0.754 (95% CI, 0.715 to 0.793) in the validation cohort. After the addition of CEA and CA 72-4, the performance of pathology-based model was significantly improved in in both the primary cohort (AUC: 0.805 (0.774-0.835) vs. 0.756 (0.722-0.789), P < 0.001) and validation cohort (AUC: 0.796 (0.758-0.835) vs. 0.754 (0.715-0.793), P < 0.001). The results of decision curve analysis revealed that using our models to predict the status of MMR would add more benefit than either the detect-all-patients scheme or the detect-none scheme.

Interpretation: The models based on simplified clinicopathological features alone or in combination with STMs can be conveniently used to facilitate the postoperative individualized prediction of MMR status in CRC patients.
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http://dx.doi.org/10.1016/j.ebiom.2020.103060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578682PMC
November 2020

MicroRNA-214-3p targets the PLAGL2-MYH9 axis to suppress tumor proliferation and metastasis in human colorectal cancer.

Aging (Albany NY) 2020 05 15;12(10):9633-9657. Epub 2020 May 15.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Evidence has shown that microRNAs (miRNAs) participate in the progression of CRC. Previous studies have indicated that miR-214-3p is abnormally expressed in various malignant tumors. However, the biological function it plays in CRC and the potential mechanism are unclear. Here, we demonstrated that miR-214-3p was obviously downregulated in CRC. Moreover, we found a strong correlation between the miR-214-3p level and tumor size and lymphatic metastasis. Furthermore, when miR-214-3p was decreased by an Lv-miR-214-3p inhibitor, the proliferation and migration of SW480 and HCT116 cells were significantly increased. As expected, the ability of proliferation and migration was significantly suppressed when miR-214-3p was overexpressed in DLD1 cells. According to the dual-luciferase reporter results, PLAGL2 was found to be a direct downstream molecule of miR-214-3p. Chromatin immunoprecipitation (CHIP) confirmed that MYH9, a well-known cytoskeleton molecule in CRC, was a direct targeting gene of PLAGL2. Silencing PLAGL2 or MYH9 could reverse the effect of a miR-214-3p inhibitor on CRC cells. In summary, our studies proved that low expression of miR-214-3p and overexpression of downstream PLAGL2 in CRC indicated a poor prognosis. MiR-214-3p suppressed the malignant behaviors of colorectal cancer by regulating the PLAGL2/MYH9 axis. MiR-214-3p might be a novel therapeutic target or prognostic marker for CRC.
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http://dx.doi.org/10.18632/aging.103233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288958PMC
May 2020

Effect of Gastrointestinal Symptoms in Patients With COVID-19.

Gastroenterology 2020 Jun 19;158(8):2294-2297. Epub 2020 Mar 19.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

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http://dx.doi.org/10.1053/j.gastro.2020.03.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7270807PMC
June 2020

PLAGL2 promotes epithelial-mesenchymal transition and mediates colorectal cancer metastasis via β-catenin-dependent regulation of ZEB1.

Br J Cancer 2020 02 12;122(4):578-589. Epub 2019 Dec 12.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: We previously demonstrated that the pleomorphic adenoma gene like-2 (PLAGL2) is involved in the pathogenesis of Hirschsprung disease. Enhanced PLAGL2 expression was observed in several malignant tumours. However, the exact function of PLAGL2 and its underlying mechanism in colorectal cancer (CRC) remain largely unknown.

Methods: Immunohistochemical analysis of PLAGL2 was performed. A series of in vitro and in vivo experiments were conducted to reveal the role of PLAGL2 in the progression of CRC.

Results: Enhanced PLAGL2 expression was significantly associated with EMT-related proteins in CRC. The data revealed that PLAGL2 promotes CRC cell proliferation, migration, invasion and EMT both in vitro and in vivo. Mechanistically, PLAGL2 promoted the expression of ZEB1. PLAGL2 enhanced the expression and nuclear translocation of β-catenin by decreasing its phosphorylation. The depletion of β-catenin neutralised the regulation of ZEB1 that was caused by enhanced PLAGL2 expression. The small-molecule inhibitor PNU-74654, also impaired the enhancement of ZEB1 that resulted from the modified PLAGL2 expression. The depletion of ZEB1 could block the biological function of PLAGL2 in CRC cells.

Conclusions: Collectively, our findings suggest that PLAGL2 mediates EMT to promote colorectal cancer metastasis via β-catenin-dependent regulation of ZEB1.
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http://dx.doi.org/10.1038/s41416-019-0679-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028997PMC
February 2020

The Ecological Water Demand of in Tibet Based on Habitat Area and Connectivity.

Int J Environ Res Public Health 2019 08 22;16(17). Epub 2019 Aug 22.

State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610065, China.

Water resource regulation is convenient for humans, but also changes river hydrology and affects aquatic ecosystems. This study combined a field investigation and two-dimensional hydrodynamic model (MIKE21) to simulate the hydrodynamic distribution from 1 March to 30 April of 2008-2013 and establish the HDI (habitat depth suitability index) and HVI (habitat velocity suitability index) based on static hydraulic conditions at typical points. Additionally, by using MIKE21 to simulate the hydraulic state in the study area under 20 flow conditions from 530-1060 m/s, and combining these states with the HCI (habitat cover type suitability index), HDI, and HVI, we simulated the WUA (weighted usable area) and habitat connectivity under different runoff regulation scenarios to study the water requirements of during the spawning period in the Yanni wetland. The results showed the following: (1) the suitable cover type was cobble and rock substrate, with nearby sandy land; furthermore, the suitable water depth was 0.5-1.5 m, and the suitable velocity was 0.1-0.9 m/s. (2) Using the proximity index to analyse the connectivity of suitable habitats, the range of ecological discharge determined by the WUA and connectivity was 424-1060 m/s. (3) Habitat quality was divided into three levels to detail the flow demand further. When the flow was 424-530 m/s or 848-1060 m/s, the WUA and connectivity generally met the requirements under natural conditions. When the flow was 530-636 m/s or 742-848 m/s, the WUA and connectivity were in a good state. When the flow was 636-742 m/s, the WUA and connectivity were in the best state. This study complements existing research on the suitability of habitat in Tibet, and introduces the connectivity index to enrich the method for calculating ecological water demand, providing a reference for resource regulation and the protection of aquatic organisms.
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http://dx.doi.org/10.3390/ijerph16173045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747338PMC
August 2019

Increased miR-214 expression suppresses cell migration and proliferation in Hirschsprung disease by interacting with PLAGL2.

Pediatr Res 2019 10 1;86(4):460-470. Epub 2019 Mar 1.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: The miR-214 has been reported to be associated with various diseases, but its involvement in the pathophysiology of Hirschsprung disease (HSCR) is almost completely unexplored.

Methods: In our study, we conducted a series of experiments to unravel the biological role of miR-214 in the pathophysiology of HSCR. qRT-PCR and western blotting were utilized to investigate the relative expression levels of miR-214, mRNAs, and proteins of related genes in colon tissues from 20 controls without HSCR and 24 patients with HSCR. The potential biological role of miR-214 in two cell lines (SKN-SH and SH-SY5Y) was assessed using the CCK8 assay, EdU staining, transwell assay, and flow cytometry. The dual-luciferase reporter assay was used to confirm PLAGL2 as a common target gene of miR-214.

Results: All results suggested that miR-214 is upregulated in HSCR tissue samples compared with controls. Additionally, we found that miR-214 could inhibit cell proliferation and migration by directly downregulating the expression of PLAGL2, and the extent of the miR-214-mediated inhibitory effects could be rescued by a PLAGL2 overexpression plasmid.

Conclusion: Our results revealed that miR-214 is indeed involved in the pathophysiology of HSCR and suppresses cell proliferation and migration by directly downregulating PLAGL2 in cell models.
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http://dx.doi.org/10.1038/s41390-019-0324-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6768286PMC
October 2019

Downregulation of DAPK1 promotes the stemness of cancer stem cells and EMT process by activating ZEB1 in colorectal cancer.

J Mol Med (Berl) 2019 01 20;97(1):89-102. Epub 2018 Nov 20.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Cancer stem cells (CSCs) and epithelial-mesenchymal transition (EMT) play an important role in the metastasis and chemoresistance in the context of colorectal cancer (CRC). Downregulation of death associated protein kinase 1 (DAPK1) may promote metastasis and chemoresistance of cancer cells through various mechanisms. However, the association between DAPK1 and CSCs or EMT has not been explored. In this study, we demonstrated that DAPK1 was associated with elevated stemness of CSCs in patients with CRC. Silencing of DAPK1 in CRC cell lines promoted the metastasis and chemoresistance due to increased stemness of CSCs and enhanced mesenchymal phenotype, an effect that was mediated via activation of the transcription factor, zinc finger E-box binding homeobox 1 (ZEB1). Blockade of this signaling pathway attenuated the stemness of CSCs and rescued the EMT process. DAPK1-ZEB1 may lie at the interface of TGF-β and WNT pathways and participate in both CSCs and EMT process. Targeted therapies aimed at DAPK1-ZEB1 pathway may inhibit the chemoresistance and metastasis of CRC. KEY MESSAGES: Downregulation of DAPK1 promotes chemoresistance and metastasis of CRC. Inhibition of DAPK1 promotes the stemness of cancer stem cells and EMT process. DAPK1-ZEB1 may lie at the interface of TGF-β and WNT pathways. DAPK1-ZEB1 participates in both CSCs and EMT process.
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http://dx.doi.org/10.1007/s00109-018-1716-8DOI Listing
January 2019

Mild Methylenetetrahydrofolate Reductase Deficiency Alters Inflammatory and Lipid Pathways in Liver.

Mol Nutr Food Res 2019 02 23;63(3):e1801001. Epub 2018 Nov 23.

Departments of Human Genetics and Pediatrics, McGill University, McGill University Health Center (MUHC), Montreal, H4A 3J1, Canada.

Scope: Dietary and genetic folate disturbances can lead to nonalcoholic fatty liver disease (NAFLD). A common variant in methylenetetrahydrofolate reductase (MTHFR 677C→T) causes mild MTHFR deficiency with lower 5-methyltetrahydrofolate for methylation reactions. The goal is to determine whether mild murine MTHFR deficiency contributes to NAFLD-related effects.

Methods And Results: Wild-type and Mthfr mice, a model for the human variant, are fed control (CD) or high-fat (HFAT) diets for 8 weeks. On both diets, MTHFR deficiency results in decreased S-adenosylmethionine, increased S-adenosylhomocysteine, and decreased betaine with reduced methylation capacity, and changes in expression of several inflammatory or anti-inflammatory mediators (Saa1, Apoa1, and Pon1). On CD, MTHFR deficiency leads to microvesicular steatosis with expression changes in lipid regulators Xbp1s and Cyp7a1. The combination of MTHFR deficiency and HFAT exacerbates changes in inflammatory mediators and introduces additional effects on inflammation (Saa2) and lipid metabolism (Nr1h4, Srebf1c, Ppara, and Crot). These effects are consistent with increased expression of pro-inflammatory HDL precursors and greater lipid accumulation. MTHFR deficiency may enhance liver injury through alterations in methylation capacity, inflammatory response, and lipid metabolism.

Conclusion: Individuals with the MTHFR variant may be at increased risk for liver disease and related complications, particularly when consuming high-fat diets.
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http://dx.doi.org/10.1002/mnfr.201801001DOI Listing
February 2019

Nuclear factor I/B promotes colorectal cancer cell proliferation, epithelial-mesenchymal transition and 5-fluorouracil resistance.

Cancer Sci 2019 Jan 8;110(1):86-98. Epub 2018 Dec 8.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Nuclear factor I/B (NFIB) is a widely studied transcription factor that participates in tumor progression; nevertheless, studies on NFIB in colorectal cancer (CRC) are limited. In our study, Western blot and RT-PCR analyses showed that NFIB was overexpressed in CRC tissues and cell lines, which was consistent with our bioinformatic analysis results. Furthermore, NFIB expression was closely related to the TNM stage of CRC. NFIB promoted cell proliferation and migration and inhibited cell apoptosis in vitro. Meanwhile, we discovered that NFIB accelerated xenograft tumor growth in vivo. In addition, NFIB weakened the sensitivity of CRC cells to 5-fluorouracil (5-FU). NFIB induced epithelial-mesenchymal transition (EMT) by upregulating snail expression, which was accompanied by decreased E-cadherin and Zo-1 expression and increasedd Vimentin expression. Because the Akt pathway plays an important role in CRC progression, we examined whether there was a correlation between NFIB and the Akt pathway in cell proliferation and migration. Our results showed that NFIB promoted cell proliferation and increased 5-FU resistance by activating the Akt pathway. In summary, our findings suggested that NFIB induced EMT of CRC cells via upregulating snail expression and promoted cell proliferation and 5-FU resistance by activating the Akt pathway.
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http://dx.doi.org/10.1111/cas.13833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317934PMC
January 2019

Strain Transfer Characteristics of Resistance Strain-Type Transducer Using Elastic-Mechanical Shear Lag Theory.

Sensors (Basel) 2018 Jul 25;18(8). Epub 2018 Jul 25.

Chinese Aeronautical Establishment, Chaoyang District, Beijing 100029, China.

The strain transfer characteristics of resistance strain gauge are theoretically investigated. A resistance strain-type transducer is modeled to be a four-layer and two-glue (FLTG) structure model, which comprises successively the surface of an elastomer sensitive element, a ground adhesive glue, a film substrate layer, an upper adhesive glue, a sensitive grids layer, and a polymer cover. The FLTG model is studied in elastic⁻mechanical shear lag theory, and the strain transfer progress in a resistance strain-type transducer is described. The strain transitional zone (STZ) is defined and the strain transfer ratio (STR) of the FLTG structure is formulated. The dependences of the STR and STZ on both the dimensional sizes of the adhesive glue and structural parameters are calculated. The results indicate that the width, thickness and shear modulus of the ground adhesive glue have a greater influence on the STZ ratio. To ensure that the resistance strain gauge has excellent strain transfer performance and low hysteresis, it is recommended that the paste thickness should be strictly controlled, and the STZ ratio should be less than 10%. Moreover, the STR strongly depends on the length and width of the sensitive grids.
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http://dx.doi.org/10.3390/s18082420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6111413PMC
July 2018

Correlation of metastasis characteristics with prognosis in gastric mixed adenoneuroendocrine carcinoma: Two case reports.

Medicine (Baltimore) 2017 Dec;96(50):e9189

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Rationale: This article is aimed to retrospect the clinicopathological data of 2 cases of gastric MANENCs. MANEC is a rare biphasic tumor type that is coexistence of dual neuroendocrine and adenocarcinoma differentiation with each composing exceeding 30% volume. Gastric MANEC have just been reported anecdotally in the literature due to their rarity and heterogeneity. According to our study, these neoplasms have 3 different metastasis patterns: only adenocarcinomatous or neuroendocrine carcinoma and both of the 2 components. We first focus on the correlation of metastasis characteristics with prognosis in gastric MANEC, which may be potential implications for the choice of chemotherapy.

Patient Concerns: The 2 cases of patient shared several symptoms: epigastric discomfort, weight loss, hematemesis, or melena.

Diagnosis: The 2 patients were diagnosis as MANEC based on the identification of histopathological analysis. In case 1, the poor differentiated adenocarcinoma accounted for 30%, the neuroendocrine part account for 70% and both of the 2 components metastasized to the lymph nodes, whereas in case 2, poorly differentiated adenocarcinoma accounted for 70%, the neuroendocrine part for 30% and only the glandular component invaded regional lymph nodes.

Interventions: The first patient underwent laparoscopic radical gastrectomy and underwent adjuvant chemotherapy, combination of cisplatin, and etoposide successfully. The second patient received radical gastronomy, and did not receive any chemotherapy due to general weakness.

Outcomes: The first patient is alive with no evidence of recurrence, and the second patient died 6 months after the operation.

Lessons: The assessment of metastatic sites should be a routine pathological practice, which is crucial for clinical decision-making and the selection of management.
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http://dx.doi.org/10.1097/MD.0000000000009189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5815743PMC
December 2017

Correlation of DAPK1 methylation and the risk of gastrointestinal cancer: A systematic review and meta-analysis.

PLoS One 2017 21;12(9):e0184959. Epub 2017 Sep 21.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: One of the critical mechanisms of gastrointestinal cancer pathogenesis is the silencing of death associated protein kinase 1 (DAPK1), which could be caused by aberrant methylation of the promoter. However, the relationship between DAPK1 methylation and the risk of gastrointestinal cancer is still controversial. Hence, we conducted this study to determine the potential correlation.

Methods: Eligible publications were searched in the Pubmed, Embase, and Cochrane Library through November 2016 according to the inclusion criteria and exclusion criteria. Revman 5.3 and Stata 12.0 software were used to analyze the relevant data regarding the association between the frequency of DAPK1 methylation and gastrointestinal cancer.

Results: A total of 22 studies with 2406 patients were included in this meta analysis. Methylation of DAPK1 was positively related with the risk of gastrointestinal cancer (odds ratio [OR] = 5.35, 95% confidence interval [CI]: 2.76-10.38, P<0.00001, random effects model). The source of heterogeneity was analyzed by sensitivity analysis and subgroup analysis. After omitting one heterogeneous study, the I2 decreased and the OR increased in pooled analysis. Also, the heterogeneity decreased most significantly in the subgroup of studies that had a sample size of less than 60 cases. Then, the correlations between DAPK1 methylation and clinicopathological features of gastrointestinal cancer were assessed. DAPK1 methylation was positively correlated with the lymph node (N) stage (positive vs. negative, OR = 1.45, 95%CI: 1.01-2.06, P = 0.04, fixed effects model) and poor differentiation (OR = 1.55, 95%CI: 1.02-2.35, P = 0.04, fixed effects model) in gastric cancer, and the association was significant among Asian patients. However, among cases of gastrointestinal cancer, the association between DAPK1 methylation and tumor (T) stage, N stage, distant metastasis (M) stage, and cancer differentiation were not statistically significant.

Conclusions: DAPK1 methylation is a potential biomarker for the early diagnosis of gastrointestinal cancer. Further analysis of the clinicopathological features indicated that aberrant methylation of DAPK1 is positively associated with the tumorigenesis of gastrointestinal cancer, and metastasis of gastric cancer.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0184959PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608298PMC
October 2017

PDGF-D promotes cell growth, aggressiveness, angiogenesis and EMT transformation of colorectal cancer by activation of Notch1/Twist1 pathway.

Oncotarget 2017 Feb;8(6):9961-9973

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Platelet-derived growth factor-D (PDGF-D) plays a crucial role in the progression of several cancers. However, its role in colorectal cancer (CRC) remains unclear. Our study showed that PDGF-D was highly expressed in CRC tissues and was positively associated with the clinicopathological features. Down-regulation of PDGF-D inhibited the tumor growth, migration and angiogenesis of SW480 cells in vitro and in vivo. Whereas up-regulation of PDGF-D promoted the malignant behaviors of HCT116 cells. Moreover, PDGF-D up-regulated the expression of Notch1 and Twist1 in CRC cells. In addition, PDGF-D expression promoted Epithelial to mesenchymal transition (EMT), which was accompanied with decreased E-cadherin and increased Vimentin expression. Consistently, PDGF-D, Notch1, and Twist1 are obviously up-regulated in transforming growth factor-beta 1 (TGF-β1) treated HCT116 cells. Since Notch1 and Twist1 play an important role in EMT and tumor progression, we examined whether there is a correlation between Notch1 and Twist1 in EMT status. Our results showed that up-regulation of Notch1 was able to rescue the effects of PDGF-D down-regulation on Twist1 expression in SW480 cells, whereas down-regulation of Notch1 reduced Twist1 expression in HCT116 cells. Furthermore, we found that Twist1 promoted EMT and aggressiveness of CRC cells. These results suggest that PDGF-D promotes tumor growth and aggressiveness of CRC, moreover, down-regulation of PDGF-D inactivates Notch1/Twist1 axis, which could reverse EMT and prevent CRC progression.
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http://dx.doi.org/10.18632/oncotarget.14283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354784PMC
February 2017

Accelerated azo dye degradation and concurrent hydrogen production in the single-chamber photocatalytic microbial electrolysis cell.

Bioresour Technol 2017 Jan 25;224:63-68. Epub 2016 Oct 25.

School of Environmental Science & Engineering, Guangxi University, Guangxi Colleges and Universities Key Laboratory of Environmental Protection, Nanning 530004, China.

The single-chamber microbial electrolysis cell constructed with a TiO-coated photocathode, termed photocatalytic microbial electrolysis cell (PMEC), was developed to accelerate methyl orange (MO) degradation and concurrent hydrogen (H) recovery under UV irradiation. Results showed that faster MO decolorization rates were achieved from the PMEC compared with those without UV irradiation or with open circuit. With increase of MO concentrations (acetate as co-substrate) from 50 to 300mg/L at an applied voltage of 0.8V, decolorization efficiencies decreased from 98% to 76% within 12h, and cyclic H production declined from 113 to 68mL. As the possible mechanism of MO degradation, bioelectrochemical reduction, co-metabolism reduction, and photocatalysis were involved; and degradation intermediates (mainly sulfanilic acid and N,N-dimethylaniline) were further degraded by OH generated from photocatalysis. This makes MO mineralization be possible in the single-chamber PMEC. Hence, the PMEC is a promising system for dyeing wastewater treatment and simultaneous H production.
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http://dx.doi.org/10.1016/j.biortech.2016.10.069DOI Listing
January 2017

Treatment of anaerobically digested swine wastewater by Rhodobacter blasticus and Rhodobacter capsulatus.

Bioresour Technol 2016 Dec 30;222:33-38. Epub 2016 Sep 30.

College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, PR China. Electronic address:

Two strains of photosynthetic bacteria, Rhodobacter blasticus and Rhodobacter capsulatus, were used in this work to investigate the feasibility of using photosynthetic bacteria for the treatment of anaerobically digested swine wastewater. The effects of crucial factors which influence the pollutants removal efficiency were also examined. Results showed that anaerobically digested swine wastewater could be treated effectively by photosynthetic bacteria. The treatment efficiency was significantly higher by the mixed photosynthetic bacteria than that by any unitary bacterium. The optimal treatment condition by mixed bacteria was inoculation of 10.0%(v/v) of the two bacteria by 1:1, initial pH of 7.0 and initial chemical oxygen demand of 4800mgL. Under these conditions, the removal rate of chemical oxygen demand was 83.3%, which was 19.3% higher than when using Rhodobacter blasticus or 10.6% higher than when using Rhodobacter capsulatus separately. This mixed photosynthetic bacteria achieved high chemical oxygen demand removal and cell yields.
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http://dx.doi.org/10.1016/j.biortech.2016.09.102DOI Listing
December 2016

Nutrient removal and lipid production by Coelastrella sp. in anaerobically and aerobically treated swine wastewater.

Bioresour Technol 2016 Sep 20;216:135-41. Epub 2016 May 20.

Hunan Hikee Environmental Technology Co., Ltd., Changsha 410001, PR China.

Coelastrella sp. QY01, a microalgae species isolated from a local pond, was identified and used for the treatment of anaerobically and aerobically treated swine wastewater (AnATSW). Microalgal growth characteristics, nutrient removal and lipid accumulation of QY01 cultivated in the initial concentration of AnATSW ranged from 63 to 319mg NH3-N/L were examined. The specific growth rate of QY01 cultivated in cultures ranged from 0.269 to 0.325day(-1) with a biomass productivity from 42.77 to 57.46mgL(-1)day(-1). Removal rates for NH3-N, TP and inorganic carbon in AnATSW at the various nutrient concentrations ranged from 90% to 100%, from 90% to 100% and from 74% to 78%, respectively. The lipid content of QY01 ranged from 22.4% to 24.8%. The lipid productivity was positive correlation with the biomass productivity. 40% AnATSW was optimal for QY01 cultivation, in which nutrient removal and productivity of biomass and lipid were maximized.
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http://dx.doi.org/10.1016/j.biortech.2016.05.059DOI Listing
September 2016

Photon-counting and analog operation of a 24-pixel photon number resolving detector based on superconducting nanowires.

Opt Express 2016 Apr;24(8):9067-76

We investigate the transition from the photon-counting to the linear operation mode in a large-dynamic range photon-number-resolving-detector (PNRD). A 24-pixel photon-number-resolving-detector, based on superconducting nanowires in a series configuration, has been fabricated and characterized. The voltage pulses, generated by the pixels, are summed up into a single readout pulse whose height is proportional to the detected photon number. The device can resolve up to twenty-five distinct output levels corresponding to the detection of n = 0-24 photons. Due to its large dynamic range, high sensitivity, high speed and wide wavelength range, this device has potential for linear detection in the few tens of photons range. We show its application in the detection of analog optical signals at frequencies up to few hundred MHz and investigate the limits related to the finite number of pixels and to the pixel's dead time.
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http://dx.doi.org/10.1364/OE.24.009067DOI Listing
April 2016

Regularised extreme learning machine with misclassification cost and rejection cost for gene expression data classification.

Int J Data Min Bioinform 2015 ;12(3):294-312

The main purpose of traditional classification algorithms on bioinformatics application is to acquire better classification accuracy. However, these algorithms cannot meet the requirement that minimises the average misclassification cost. In this paper, a new algorithm of cost-sensitive regularised extreme learning machine (CS-RELM) was proposed by using probability estimation and misclassification cost to reconstruct the classification results. By improving the classification accuracy of a group of small sample which higher misclassification cost, the new CS-RELM can minimise the classification cost. The 'rejection cost' was integrated into CS-RELM algorithm to further reduce the average misclassification cost. By using Colon Tumour dataset and SRBCT (Small Round Blue Cells Tumour) dataset, CS-RELM was compared with other cost-sensitive algorithms such as extreme learning machine (ELM), cost-sensitive extreme learning machine, regularised extreme learning machine, cost-sensitive support vector machine (SVM). The results of experiments show that CS-RELM with embedded rejection cost could reduce the average cost of misclassification and made more credible classification decision than others.
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http://dx.doi.org/10.1504/ijdmb.2015.069657DOI Listing
November 2015

Prediction of multiple resonance characteristics by an extended resistor-inductor-capacitor circuit model for plasmonic metamaterials absorbers in infrared.

Opt Lett 2015 Oct;40(19):4432-5

The resonance characteristics of plasmonic metamaterials absorbers (PMAs) are strongly dependent on geometric parameters. A resistor-inductor-capacitor (RLC) circuit model has been extended to predict the resonance wavelengths and the bandwidths of multiple magnetic polaritons modes in PMAs. For a typical metallic-dielectric-metallic structure absorber working in the infrared region, the developed model describes the correlation between the resonance characteristics and the dimensional sizes. In particular, the RLC model is suitable for not only the fundamental resonance mode, but also for the second- and third-order resonance modes. The prediction of the resonance characteristics agrees fairly well with those calculated by the finite-difference time-domain simulation and the experimental results. The developed RLC model enables the facilitation of designing multi-band PMAs for infrared radiation detectors and thermal emitters.
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http://dx.doi.org/10.1364/OL.40.004432DOI Listing
October 2015

Superconducting series nanowire detector counting up to twelve photons.

Opt Express 2014 Feb;22(3):3475-89

We demonstrate a superconducting photon-number-resolving detector capable of resolving up to twelve photons at telecommunication wavelengths. It is based on a series array of twelve superconducting NbN nanowire elements, each connected in parallel with an integrated resistor. The photon-induced voltage signals from the twelve elements are summed up into a single readout pulse with a height proportional to the detected photon number. Thirteen distinct output levels corresponding to the detection of n = 0-12 photons are observed experimentally. A detailed analysis of the linearity and of the excess noise shows the potential of scaling to an even larger dynamic range.
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http://dx.doi.org/10.1364/OE.22.003475DOI Listing
February 2014

Identification and analysis of MKK and MPK gene families in canola (Brassica napus L.).

BMC Genomics 2013 Jun 11;14:392. Epub 2013 Jun 11.

State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.

Background: Eukaryotic mitogen-activated protein kinase (MAPK/MPK) signaling cascades transduce and amplify environmental signals via three types of reversibly phosphorylated kinases to activate defense gene expression. Canola (oilseed rape, Brassica napus) is a major crop in temperate regions. Identification and characterization of MAPK and MAPK kinases (MAPKK/MKK) of canola will help to elucidate their role in responses to abiotic and biotic stresses.

Results: We describe the identification and analysis of seven MKK (BnaMKK) and 12 MPK (BnaMPK) members from canola. Sequence alignments and phylogenetic analyses of the predicted amino acid sequences of BnaMKKs and BnaMPKs classified them into four different groups. We also examined the subcellular localization of four and two members of BnaMKK and BnaMPK gene families, respectively, using green fluorescent protein (GFP) and, found GFP signals in both nuclei and cytoplasm. Furthermore, we identified several interesting interaction pairs through yeast two-hybrid (Y2H) analysis of interactions between BnaMKKs and BnaMPKs, as well as BnaMPK and BnaWRKYs. We defined contiguous signaling modules including BnaMKK9-BnaMPK1/2-BnaWRKY53, BnaMKK2/4/5-BnaMPK3/6-BnaWRKY20/26 and BnaMKK9-BnaMPK5/9/19/20. Of these, several interactions had not been previously described in any species. Selected interactions were validated in vivo by a bimolecular fluorescence complementation (BiFC) assay. Transcriptional responses of a subset of canola MKK and MPK genes to stimuli including fungal pathogens, hormones and abiotic stress treatments were analyzed through real-time RT-PCR and we identified a few of BnaMKKs and BnaMPKs responding to salicylic acid (SA), oxalic acid (OA), Sclerotinia sclerotiorum or other stress conditions. Comparisons of expression patterns of putative orthologs in canola and Arabidopsis showed that transcript expression patterns were generally conserved, with some differences suggestive of sub-functionalization.

Conclusions: We identified seven MKK and 12 MPK genes from canola and examined their phylogenetic relationships, transcript expression patterns, subcellular localization, and protein-protein interactions. Not all expression patterns and interactions were conserved between canola and Arabidopsis, highlighting the limitations of drawing inferences about crops from model species. The data presented here provide the first systematic description of MKK-MPK-WRKY signaling modules in canola and will further improve our understanding of defense responses in general and provide a basis for future crop improvement.
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http://dx.doi.org/10.1186/1471-2164-14-392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701561PMC
June 2013

Ultrasensitive N-photon interferometric autocorrelator.

Phys Rev Lett 2013 Mar 28;110(13):133605. Epub 2013 Mar 28.

COBRA Research Institute, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.

We demonstrate a novel method to measure Nth-order (N=1,2,3,4) interferometric autocorrelation with high sensitivity and temporal resolution. It is based on the combination of linear absorption and nonlinear detection in a superconducting nanodetector, providing much higher efficiency than methods based on all-optical nonlinearities. Its temporal resolution is only limited by the quasiparticle energy relaxation time, which is directly measured to be in the 20 ps range for the NbN films used in this work. We present a general model of interferometric autocorrelation with these nonlinear detectors and discuss the comparison with other approaches and possible improvements.
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http://dx.doi.org/10.1103/PhysRevLett.110.133605DOI Listing
March 2013
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