Publications by authors named "Zi-Yu Li"

96 Publications

M1-type microglia can induce astrocytes to deposit chondroitin sulfate proteoglycan after spinal cord injury.

Neural Regen Res 2022 May;17(5):1072-1079

Department of Orthopedics, The Second Hospital of Anhui Medical University, Hefei, Anhui Province, China.

After spinal cord injury (SCI), astrocytes gradually migrate to and surround the lesion, depositing chondroitin sulfate proteoglycan-rich extracellular matrix and forming astrocytic scar, which limits the spread of inflammation but hinders axon regeneration. Meanwhile, microglia gradually accumulate at the lesion border to form microglial scar and can polarize to generate a pro-inflammatory M1 phenotype or an anti-inflammatory M2 phenotype. However, the effect of microglia polarization on astrocytes is unclear. Here, we found that both microglia (CX3CR1) and astrocytes (GFAP) gathered at the lesion border at 14 days post-injury (dpi). The microglia accumulated along the inner border of and in direct contact with the astrocytes. M1-type microglia (iNOSCX3CR1) were primarily observed at 3 and 7 dpi, while M2-type microglia (Arg1CX3CR1) were present at larger numbers at 7 and 14 dpi. Transforming growth factor-β1 (TGFβ1) was highly expressed in M1 microglia in vitro, consistent with strong expression of TGFβ1 by microglia in vivo at 3 and 7 dpi, when they primarily exhibited an M1 phenotype. Furthermore, conditioned media from M1-type microglia induced astrocytes to secrete chondroitin sulfate proteoglycan in vitro. This effect was eliminated by knocking down sex-determining region Y-box 9 (SOX9) in astrocytes and could not be reversed by treatment with TGFβ1. Taken together, our results suggest that microglia undergo M1 polarization and express high levels of TGFβ1 at 3 and 7 dpi, and that M1-type microglia induce astrocytes to deposit chondroitin sulfate proteoglycan via the TGFβ1/SOX9 pathway. The study was approved by the Institutional Animal Care and Use Committee of Anhui Medical University, China (approval No. LLSC20160052) on March 1, 2016.
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http://dx.doi.org/10.4103/1673-5374.324858DOI Listing
May 2022

Anatomical Study on the Safety of Anterior Cervical Craniovertebral Fusion with Clival Screw Placement in Children Aged 1-6 Years.

Int J Gen Med 2021 16;14:5787-5794. Epub 2021 Sep 16.

Department of Human Anatomy, Inner Mongolia Medical University, Hohhot, People's Republic of China.

Objective: This study aimed to investigate the safety of clival screw placement in children aged 1-6 years.

Methods: The cranial computed tomography data of 92 children aged 1-6 years were divided into three groups, according to age, for three-dimensional reconstruction. Three clival screw placement points were defined: (1) median point A at the middle and upper third of the tripartite distance from the spheno-occipital synchondrosis to the base of the skull; (2, 3) critical points B and C on the horizontal line with point A, where the screw placement passage was parallel to the sagittal plane. Parameters such as the passage length and angle were measured for horizontal, vertical facial, and extreme screw placement.

Results: The length parameters of the clival screw placement increased with age, and the screw passage length was the shortest for the vertical facial type in each age group. There were significant differences in all three groups between the length of screw placement in the vertical bone surface, the length of screw placement in the horizontal direction, and the length of screw placement on the limit at points A, B, and C ( < 0.05); the length of screw placement on the vertical bone surface was the shortest. There was no significant difference between the horizontal screw length at point A and the extreme screw length ( > 0.05). The difference between the horizontal screw length and the extreme screw length in the groups aged 1-2 years and 5-6 years was statistically significant ( < 0.05), and the horizontal screw length was longer.

Conclusion: The cranial slope of children aged 1-6 years has the morphological basis for the placement of 3.5 mm screws, and each placement point has a safe angle range for screw placement.
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http://dx.doi.org/10.2147/IJGM.S328496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454928PMC
September 2021

Dual Iron Sites in Activation of N by Iron-Sulfur Cluster Anions FeS and FeS.

J Phys Chem Lett 2021 Sep 17:9269-9274. Epub 2021 Sep 17.

State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

Inspired by the fact that the active centers of natural nitrogenases are polynuclear iron-sulfur clusters, the reactivity of isolated iron-sulfur clusters toward N has received considerable attention to gain fundamental insights into the activation of the N≡N triple bond. Herein, a series of gas-phase iron-sulfur cluster anions FeS ( = 1-8, 0-) were prepared and their reactivities toward N were investigated systematically by mass spectrometry. Among the 44 investigated clusters, only FeS and FeS showed superior reactivity toward N. Theoretical results revealed that N binds molecularly to the iron sites of FeS in a common end-on coordination mode with an unprecedented back-donation interaction from the localized - bonding orbitals of Fe-Fe sites to the π* antibonding orbitals of N. This is the first example to disclose the significant contribution of the dual metal sites rather than the single metal atom to N adsorption in the prevalent end-on binding mode.
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http://dx.doi.org/10.1021/acs.jpclett.1c02683DOI Listing
September 2021

Hepatic artery injection of I-metuximab combined with transcatheter arterial chemoembolization for unresectable hepatocellular carcinoma: a prospective non-randomized, multicenter clinical trial.

J Nucl Med 2021 Sep 2. Epub 2021 Sep 2.

College of Military Preventive Medicine, Fourth Military Medical University,, China.

This prospective non-randomized, multicenter clinical trial was performed to investigate efficacy and safety of I-labeled metuximab in adjuvant treatment of unresectable hepatocellular carcinoma. Patients were assigned to treatment with transcatheter arterial chemoembolization (TACE) combined with I-metuximab or TACE alone. The primary outcome was overall tumor recurrence. The secondary outcomes were safety and overall survival. The median time to tumor recurrence was 6 months in the TACE+I-metuximab group ( = 160) and 3 months in the TACE group ( = 160) (hazard ratio, 0.55; 95% confidence interval, 0.43 to 0.70; < 0.001). The median overall survival was 28 months in the TACE+I-metuximab group and 19 months in the TACE group (hazard ratio, 0.62; 95% confidence interval, 0.47 to 0.82; = 0.001). TACE+I-metuximab showed a greater anti-recurrence benefit, significantly improved the 5-year survival of patients with advanced hepatocellular carcinoma, and was well tolerated by patients.
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http://dx.doi.org/10.2967/jnumed.121.262136DOI Listing
September 2021

Water Gas Shift Reaction Catalyzed by Rhodium-Manganese Oxide Cluster Anions.

J Phys Chem Lett 2021 Sep 31;12(35):8513-8520. Epub 2021 Aug 31.

State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Fundamental understanding of the nature of active sites in real-life water gas shift (WGS) catalysts that can convert CO and HO into CO and H is crucial to engineer related catalysts performing under ambient conditions. Herein, we identified that the WGS reaction can be, in principle, catalyzed by rhodium-manganese oxide clusters RhMnO in the gas phase at room temperature. This is the first example of the construction of such a potential catalysis in cluster science because it is challenging to discover clusters that can abstract the oxygen from HO and then supply the anchored oxygen to oxidize CO. The WGS reaction was characterized by mass spectrometry, photoelectron spectroscopy, and quantum-chemical calculations. The coordinated oxygen in RhMnO is paramount for the generation of an electron-rich Mn-Rh bond that is critical to capture and reduce HO and giving rise to a polarized Rh-Rh bond that functions as the real redox center to drive the WGS reaction.
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http://dx.doi.org/10.1021/acs.jpclett.1c02267DOI Listing
September 2021

Exosome-derived noncoding RNAs in gastric cancer: functions and clinical applications.

Mol Cancer 2021 07 30;20(1):99. Epub 2021 Jul 30.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital & Institute, No. 52 Fu-Cheng Road, Hai-Dian District, Beijing, 100142, P.R. China.

Exosomes are a subpopulation of the tumour microenvironment (TME) that transmit various biological molecules to promote intercellular communication. Exosomes are derived from nearly all types of cells and exist in all body fluids. Noncoding RNAs (ncRNAs) are among the most abundant contents in exosomes, and some ncRNAs with biological functions are specifically packaged into exosomes. Recent studies have revealed that exosome-derived ncRNAs play crucial roles in the tumorigenesis, progression and drug resistance of gastric cancer (GC). In addition, regulating the expression levels of exosomal ncRNAs can promote or suppress GC progression. Moreover, the membrane structures of exosomes protect ncRNAs from degradation by enzymes and other chemical substances, significantly increasing the stability of exosomal ncRNAs. Specific hallmarks within exosomes that can be used for exosome identification, and specific contents can be used to determine their origin. Therefore, exosomal ncRNAs are suitable for use as diagnostic and prognostic biomarkers or therapeutic targets. Regulating the biogenesis of exosomes and the expression levels of exosomal ncRNAs may represent a new way to block or eradicate GC. In this review, we summarized the origins and characteristics of exosomes and analysed the association between exosomal ncRNAs and GC development.
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http://dx.doi.org/10.1186/s12943-021-01396-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323226PMC
July 2021

Neoadjuvant chemoradiotherapy followed by laparoscopic distal gastrectomy in advanced gastric cancer: A case report and review of literature.

World J Clin Cases 2021 Apr;9(11):2542-2554

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Gastrointestinal Cancer Center, Peking University Cancer Hospital & Institute, Beijing 100142, China.

Background: The laparoscopic technique has been widely applied for early gastric cancer, with the advantages of minimal invasion and quick recovery. However, there is no report about the safety and oncological outcome of laparoscopic gastrectomy with D2 lymph node dissection for patients after neoadjuvant chemoradiotherapy.

Case Summary: A 60-year-old man was diagnosed with advanced distal gastric cancer, cT4aN1M0 stage III. The neoadjuvant chemoradiotherapy was performed based on the regimen of gross tumor volume 50G y/25 f and clinical target volume 45 Gy/25 f, as well as concurrent S-1 60 mg Bid. Then laparoscopic distal gastrectomy with D2 lymph node dissection was undertaken successfully for him after achieving partial response evaluated by radiological examination. The patient recovered smoothly without moderate or severe postoperative complications. The postoperative pathological stage was ypT3N0M0 with American Joint Committee on Cancer tumor regression grade 1. He was still in good condition after 5 years of follow-up.

Conclusion: Neoadjuvant chemoradiotherapy followed by laparoscopic technique could be applicable and may achieve satisfactory oncological outcomes. Our finding requires further validation by cohort studies.
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http://dx.doi.org/10.12998/wjcc.v9.i11.2542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040168PMC
April 2021

Prognostic predictors in patients with sepsis after gastrointestinal tumor surgery: A retrospective study.

World J Gastrointest Surg 2021 Mar;13(3):256-266

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Gastrointestinal Cancer Center, Peking University Cancer Hospital and Institute, Beijing 100142, China.

Background: There have been different reports on mortality of sepsis; however, few focus on the prognosis of patients with sepsis after surgery.

Aim: To study the clinical features and prognostic predictors in patients with sepsis after gastrointestinal tumor surgery in intensive care unit (ICU).

Methods: We retrospectively screened patients who underwent gastrointestinal tumor surgery at Peking University Cancer Hospital from January 2015 to December 2019. Among them, 181 patients who were diagnosed with sepsis in ICU were included in our study. Survival was analysed by the Kaplan-Meier method. Univariate and multivariate adjusted analyses were performed to identify predictors of prognosis.

Results: The 90-d all-cause mortality rate was 11.1% in our study. Univariate analysis showed that body mass index (BMI), shock within 48 h after ICU admission, leukocyte count, lymphocyte to neutrophil ratio, international normalized ratio, creatinine, procalcitonin, lactic acid, oxygenation index, and sequential organ failure assessment (SOFA) score within 24 h after ICU admission might be all significantly associated with the prognosis of sepsis after gastrointestinal tumor surgery. In multiple analysis, we found that BMI ≤ 20 kg/m, lactic acid after ICU admission, and SOFA score within 24 h after ICU admission might be independent risk predictors of the prognosis of sepsis after gastrointestinal tumor surgery. Compared with SOFA score, SOFA score combined with BMI and lactic acid might have higher predictive ability (area under the receiver operating characteristic curve, 0.859; 95% confidence interval, 0.789-0.929).

Conclusion: Lactic acid and SOFA score within 24 h after ICU admission are independent risk predictors of the prognosis of sepsis after gastrointestinal tumor surgery. SOFA score combined with BMI and lactic acid might have good predictive value.
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http://dx.doi.org/10.4240/wjgs.v13.i3.256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992996PMC
March 2021

Dinitrogen and Carbon Dioxide Activation to Form C-N Bonds at Room Temperature: A New Mechanism Revealed by Experimental and Theoretical Studies.

J Phys Chem Lett 2021 Apr 1;12(14):3490-3496. Epub 2021 Apr 1.

Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 102488, China.

In light of the current energy requirements, the conversion of CO and N into useful C-N bond-containing products under mild conditions has become an area of intense research. However, the inert nature of N and CO renders their coupling extremely challenging. Herein, nitrogen and carbon atoms originating from N and CO, respectively, are fixed sequentially by NbH anions in the gas phase at room temperature. Isocyanate and NbOCN anions were formed under thermal collision conditions, thus achieving the formation of new C-N bonds directly from simple N and CO. The anion structures and reaction details were studied by mass spectrometry, photoelectron spectroscopy, and quantum chemical calculations. A novel N activation mode (metal-ligand activation, MLA) and a related mechanism for constructing C-N bonds mediated by a single non-noble metal atom are proposed. In this MLA mode, the C atom originating from CO serves as an electron reservoir to accept and donate electrons.
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http://dx.doi.org/10.1021/acs.jpclett.1c00183DOI Listing
April 2021

Preliminary results of simultaneous integrated boost intensity-modulated radiation therapy based neoadjuvant chemoradiotherapy on locally advanced rectal cancer with clinically suspected positive lateral pelvic lymph nodes.

Ann Transl Med 2021 Feb;9(3):217

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing, China.

Background: Lateral pelvic lymph node (LPLN) is approximately 11-14% and always associated with poorer prognosis. This study investigated the efficacy and safety of simultaneous integrated boost intensity-modulated radiation therapy (SIB-IMRT) based on neoadjuvant chemoradiotherapy (NCRT) on locally advanced rectal cancer (LARC) patients with clinically suspected positive LPLNs.

Methods: We retrospectively screened distal LARC patients with NCRT in our center from May 2016 and June 2019. The diagnostic criteria of positive LPLN were nodes of over 7 mm in short axis and irregular border or mixed-signal intensity. All patients with clinically suspected positive LPLN received 56-60 Gy SIB-IMRT in the LPLN area. Concurrent chemotherapy regimens were capecitabine as monotherapy treatment or in combination with oxaliplatin. The toxicities, local-regional recurrence (LRR), and disease-free survival (DFS) were investigated.

Results: Fifty-two eligible patients with clinically suspected positive LPLN were screened and analyzed. The median distance from the distal tumor to the anal verge was 4 cm (range, 0-8 cm), while magnetic resonance imaging (MRI) analysis revealed the median short diameter of the pelvic LPLN to be 8 mm (range, 7-20 mm). There were 28 (53.8%) mesorectal fascia (MRF) positive and 22 (42.3%) extramural venous invasion (EMVI) positive patients. A radiotherapy dose of 41.8 Gy was administered to the pelvic area, while the LPLN received a median SIB dose of 60.0 Gy (range, 56-60 Gy) across 22 fractions. Synchronous capecitabine with or without oxaliplatin was administered during radiotherapy. In summary, 15 (28.8%) patients displayed grade 2-3 radiation-related toxicity, 8 (15.4%) patients underwent additional LPLN dissection, and positive nodes (26 nodes in total) were not observed. One patient suffered a LLR in the presacral region. The median follow-up duration was 21.2 months (range, 4.7-45.0 months), while the duration of 1- and 2-year DFS were 89.9% and 74.6%, respectively. Patients did not display LPLN recurrence.

Conclusions: The safety and efficacy of SIB-IMRT on clinically suspected positive LPLN of LARC patients were deemed acceptable. Patients did not exhibit in-field LPLN recurrence after NCRT combined with single total mesorectal excision (TME).
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http://dx.doi.org/10.21037/atm-20-4040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940951PMC
February 2021

Role of CT in the prediction of pathological complete response in gastric cancer after neoadjuvant chemotherapy.

Abdom Radiol (NY) 2021 07 10;46(7):3011-3018. Epub 2021 Feb 10.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiology, Peking University Cancer Hospital & Institute, No. 52, Fucheng Road, Haidian District, Beijing, 100142, China.

Objective: To explore which computed tomography (CT) features can predict pathological complete response (pCR) (ypT0N0) after neoadjuvant chemotherapy (NAC) in patients with gastric adenocarcinoma (GC).

Materials And Methods: This study reviewed an institutional database of patients who underwent resection of GC after NAC and identified patients with pCR from January 2010 to December 2013. The correlations between pre-chemotherapy and post-chemotherapy CT features and pCR were analyzed.

Results: Eleven of 199 patients with GC who achieved ypT0N0 status after NAC were classified as the pCR group in this study. After matching pCR (n = 11) and non-pCR patients (n = 44) in the ratio of 1:4, a total of 55 cases were analyzed. The binary logistic regression analysis showed that the post-chemotherapy short diameter of the largest lymph node and tumor thickness ratio reduction were independent predictors of pCR, with an area under the curve (AUC) of 0.94 on the receiver operating characteristic (ROC) curve analysis.

Conclusion: Two CT features, including the short diameter of the largest lymph node post-chemotherapy and tumor thickness ratio reduction, are good predictors of pCR after NAC in patients with GC.
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http://dx.doi.org/10.1007/s00261-021-02967-3DOI Listing
July 2021

EM-2 inhibited autophagy and promoted G/M phase arrest and apoptosis by activating the JNK pathway in hepatocellular carcinoma cells.

Acta Pharmacol Sin 2021 Jul 14;42(7):1139-1149. Epub 2020 Dec 14.

Department of Biochemistry, Basic Medical College, Jinan University, Guangzhou, 510632, China.

This study aimed to investigate the inhibitory effect of EM-2, a natural active monomer purified from Elephantopusmollis H.B.K., on the proliferation of human hepatocellular carcinoma cells and the molecular mechanism involved. The results from the MTT assay revealed that EM-2 significantly inhibited the proliferation of human hepatocellular carcinoma (HCC) cells in a dose-dependent manner but exhibited less cytotoxicity to the normal liver epithelial cell line LO2. EdU staining and colony formation assays further confirmed the inhibitory effect of EM-2 on the proliferation of Huh-7 hepatocellular carcinoma cells. According to the RNA sequencing and KEGG enrichment analysis results, EM-2 markedly activated the MAPK pathway in Huh-7 cells, and the results of Western blotting further indicated that EM-2 could activate the ERK and JNK pathways. Meanwhile, EM-2 induced apoptosis in a dose-dependent manner and G/M phase arrest in Huh-7 cells, which could be partially reversed when treated with SP600125, a JNK inhibitor. Further study indicated that EM-2 induced endoplasmic reticulum stress and blocked autophagic flux in Huh-7 cells by inhibiting autophagy-induced lysosome maturation. Inhibition of autophagy by bafilomycin A1 could reduce cell viability and increase the sensitivity of Huh-7 cells to EM-2. In conclusion, our findings revealed that EM-2 not only promoted G/M phase arrest and activated ER stress but also induced apoptosis by activating the JNK pathway and blocked autophagic flux by inhibiting autolysosome maturation in Huh-7 hepatocellular carcinoma cells. Therefore, EM-2 is a potential therapeutic drug with promising antitumor effects against hepatocellular carcinoma and fewer side effects.
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http://dx.doi.org/10.1038/s41401-020-00564-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208995PMC
July 2021

MicroRNAs of bone marrow mesenchymal stem cell-derived exosomes regulate acute myeloid leukemia cell proliferation and apoptosis.

Chin Med J (Engl) 2020 Dec;133(23):2829-2839

Department of Prevention and Health, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, China.

Background: Acute myeloid leukemia (AML) is a malignant hematological disease, originating from hematopoiesis stem cell differentiation obstruction and clonal proliferation. New reagents or biologicals for the treatment of AML are urgently needed, and exosomes have been identified as candidate biomarkers for disease diagnosis and prognosis. This study aimed to investigate the effects of exosomes from bone marrow mesenchymal stem cells (BMSCs) on AML cells as well as the underlying microRNA (miRNA)-mediated mechanisms.

Methods: Exosomes were isolated using a precipitation method, followed by validation using marker protein expression and nanoparticle tracking analysis. Differentially expressed miRNAs were identified by deep RNA sequencing and confirmed by quantitative real-time polymerase chain reaction (qPCR). Cell proliferation was assessed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt method, and cell cycle progression and apoptosis were detected by flow cytometry. Functional gene expression was analyzed by qPCR and Western blotting (WB). Significant differences were determined using Student's t test or analysis of variance.

Results: BMSCs-derived exosomes effectively suppressed cell proliferation (both P < 0.0001 at 10 and 20 μg/mL) and cell cycle progression (P < 0.01 at G0-G1 stage), and also significantly enhanced cell apoptosis (P < 0.001) in KG-1a cells. There were 1167 differentially expressed miRNAs obtained from BMSCs-derived exosomes compared with KG-1a cell-derived exosomes (P < 0.05). Knockdown of hsa-miR-124-5p in BMSCs abrogated the effects of BMSCs-derived exosomes in regulating KG-1a such as the change in cell proliferation (both P < 0.0001 vs. normal KG-1a cell [NC] at 48 and 72 h). KG-1a cells treated with BMSCs-derived exosomes suppressed expression of structural maintenance of chromosomes 4 (P < 0.001 vs. NC by qPCR and P < 0.0001 vs. NC by WB), which is associated with the progression of various cancers. This BMSCs-derived exosomes effect was significantly reversed with knockdown of hsa-miR-124-5p (P < 0.0001 vs. NC by WB).

Conclusions: BMSCs-derived exosomes suppress cell proliferation and cycle progression and promote cell apoptosis in KG-1a cells, likely acting through hsa-miR-124-5p. Our study establishes a basis for a BMSCs-derived exosomes-based AML treatment.
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http://dx.doi.org/10.1097/CM9.0000000000001138DOI Listing
December 2020

Health economic evaluation of patients with sepsis after gastrointestinal tumor surgery-a cost consequences analysis in China.

J Gastrointest Oncol 2020 Oct;11(5):894-898

Gastrointestinal Cancer Center, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital & Institute, Beijing, China.

Background: The purpose of this study was to evaluate the health economics of patients with sepsis after gastrointestinal tumor operation in ICU.

Methods: This case-control study used 1:1 propensity-score (PS) matched method and patients were matched according to tumor type, age and gender. The study group was composed of 181 patients with sepsis after operation of gastrointestinal tumor in ICU, while the control group was composed of 181 patients without sepsis after operation of gastrointestinal tumor. The medical expenses and length of stay in the hospital of these patients were analyzed.

Results: The median of the total hospitalization cost for the study group was $26,038, which was 1.7 times of the control group (P<0.001). The costs of drugs, laboratory test, examination, treatment, operation, anesthesia, materials, ward and other costs in the study group were higher than those in the control group (P<0.001). The median length of stay in the hospital in the study group was 26 days, which were 12 days longer than that of the control group (P<0.001). However, there was no significant difference in daily average cost between the two groups (P=0.103).

Conclusions: In ICU, patients with sepsis after operation of gastrointestinal tumor increased the cost of hospitalization and prolonged the length of stay in the hospital than those without sepsis.
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http://dx.doi.org/10.21037/jgo-20-242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7657822PMC
October 2020

Dinitrogen Activation and Functionalization by Heteronuclear Metal Cluster Anions FeVC at Room Temperature.

J Phys Chem Lett 2020 Dec 12;11(23):9990-9994. Epub 2020 Nov 12.

State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

It is of great importance to study the mechanisms to activate dinitrogen (N), the very inert molecule, under mild conditions. Gas-phase metal clusters are being actively generated to react with N to identify new reaction types and mechanisms. Herein, an unprecedented, mechanistically unique metal atom (Fe or V) ejection in the thermal reaction of FeVC with N has been identified using mass spectrometry, photoelectron imaging spectroscopy, and quantum chemistry calculations. Strong evidence suggests that the complete cleavage of the N≡N triple bond and subsequent functionalization of two N atoms via C-N coupling were achieved in this reaction. The complementary cooperation between V atoms with strong electron-donating ability and an Fe atom with large electron-withdrawing ability as well as the geometric flexibility of the Fe-V-V ring drives the whole reaction. The important role of C ligands in N≡N cleavage was also revealed. This study emphasizes the importance of heteronuclear metal systems for N fixation.
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http://dx.doi.org/10.1021/acs.jpclett.0c02921DOI Listing
December 2020

Reactivity of Neutral Tantalum Sulfide Clusters TaS ( = 0-4) with N.

J Phys Chem A 2020 Sep 9;124(38):7749-7755. Epub 2020 Sep 9.

State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

Nitrogen (N) fixation is a challenging and vital issue in chemistry. Inspired by the fact that the active sites of nitrogenases are polynuclear metal sulfide clusters, the reactivity of gas-phase metal sulfide clusters toward N has received considerable attention to gain fundamental insights into nitrogen fixation. Herein, neutral tantalum sulfide clusters have been prepared and their reactivity toward N has been investigated by mass spectrometry in conjunction with density functional theory (DFT) calculations. The experimental results showed that TaS ( = 0-3) could adsorb N, while TaS was inert to N The DFT calculations revealed that the complete cleavage of the N≡N bond on the trinuclear metal center in the TaS/N reaction systems was overall barrierless under thermal collision conditions. The sulfur ligands can facilitate the approaching of N toward the metal center but weaken the electron-donating ability of the metal center. The inertness of TaS is ascribed to the electron-deficient state of Ta in TaS and the least effective orbital interaction in the TaS/N couple. This study provides new insights into the ligand effect on the interaction of the metal clusters with N
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http://dx.doi.org/10.1021/acs.jpca.0c06462DOI Listing
September 2020

Reappraise role of No. 10 lymphadenectomy for proximal gastric cancer in the era of minimal invasive surgery during total gastrectomy: a pooled analysis of 4 prospective trial.

Gastric Cancer 2021 Jan 26;24(1):245-257. Epub 2020 Jul 26.

Department of Gastric Surgery, Fujian Medical University Union Hospital, No. 29 Xinquan Road, Fuzhou, 350001, Fujian, China.

Background: For patients with locally advanced proximal gastric cancer (LAPGC), the individualized selection of patients with highly suspected splenic hilar (No. 10) lymph node (LN) metastasis to undergo splenic hilar lymphadenectomy, is a clinical dilemma. This study aimed to re-evaluate the feasibility and safety of laparoscopic spleen-preserving splenic hilar lymphadenectomy (LSPSHL) and to identify the population who would benefit from it.

Methods: A total of 1068 patients (D2 group = 409; D2 + No. 10 group = 659) who underwent laparoscopic total gastrectomy from four prospective trials between January 2015 and July 2019 were analyzed.

Results: No significant difference in the incidence (16.9% vs. 16.4%; P = 0.837) of postoperative complications were found between the two groups. The metastasis rate of No. 10 LN among patients in the D2 + No. 10 group was 10.3% (68/659). Based on the decision tree, patients with LAPGC with tumor invading the greater curvature (Gre), patients with non-Gre-invading LAPGC with a tumor size > 5 cm and clinical positive locoregional LNs were defined as the high-priority No. 10 dissection group. The metastasis rate of No. 10 LNs in the high-priority group was 19.4% (41/211). In high-priority group, the 3-year overall survival of the D2 + No. 10 group was better than that of the D2 group (74.4% vs. 42.1%; P = 0.005), and the therapeutic index of No. 10 was higher than the indices of most suprapancreatic stations.

Conclusions: LSPSHL for LAPGC is safe and feasible when performed by experienced surgeons. LSPSHL could be recommended for the high-priority group patients even without invasion of the Gre.
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http://dx.doi.org/10.1007/s10120-020-01110-3DOI Listing
January 2021

Short- and Long-Term Outcomes after Laparoscopic Versus Open Gastrectomy for Elderly Gastric Cancer Patients: A Systematic Review and Meta-Analysis.

J Laparoendosc Adv Surg Tech A 2020 Jul 29;30(7):713-722. Epub 2020 May 29.

Department of Gastrointestinal Surgery, Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Peking University Cancer Hospital and Institute, Beijing, China.

With the rapid aging of global population, the number of elderly patients with gastric cancer is increasing. This study aimed to evaluate short- and long-term outcomes after laparoscopic gastrectomy (LG) versus open gastrectomy (OG) in elderly gastric cancer patients. We searched PubMed, EMBASE, and the Cochrane library databases from January 1994 to May 2019. Surgical safety, postoperative complications, number of harvested lymph nodes, and overall survival rate were included and analyzed. The qualities of the included studies were evaluated by Newcastle-Ottawa Quality Assessment Scale. The evidence of outcomes was evaluated using the GRADE approach. The Review Manager 5.3 (Cochrane, London, UK) and Stata 14.0 (StataCorp., College Station, Texas) were used to analyze the outcomes. Thirteen studies containing 4768 elderly patients with gastric cancer were included in this meta-analysis. LG was more favorable than OG in terms of overall postoperative morbidity (odds ratio [OR]: 0.56; 95% confidence interval [CI]: 0.44 to 0.70;  < .00001), the postoperative stay (standardized mean difference [SMD]: -0.56; 95% CI: -0.76 to (-0.37);  < .00001), and the number of harvested lymph nodes (SMD: 0.19; 95% CI: 0.09 to 0.29;  = .0003). No significant difference was found in anastomotic leakage rate (OR: 0.82; 95% CI: 0.59 to 1.12;  = .21), mental disease (OR: 0.79; 95% CI: 0.44 to 1.44;  = .44), or overall survival rate ( = .62) between two groups. However, in the subgroup with a cutoff age of 80 years, the anastomotic leakage rate was higher in LG (OR: 10.27; 95% CI: 1.31 to 80.35;  = .03). LG was more favorable than OG in the elderly patients <80 years old with gastric cancer.
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http://dx.doi.org/10.1089/lap.2019.0778DOI Listing
July 2020

A Facile N≡N Bond Cleavage by the Trinuclear Metal Center in Vanadium Carbide Cluster Anions VC.

J Am Chem Soc 2020 Jun 8;142(24):10747-10754. Epub 2020 Jun 8.

CAS Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

Cleavage of the triple N≡N bond by metal clusters is of fundamental interest and practical importance in nitrogen fixation. Previous studies of N≡N bond cleavage by gas-phase metal clusters emphasized the importance of the dinuclear metal centers. Herein, the dissociative adsorption of N and subsequent C-N coupling on trinuclear carbide cluster anions VC under thermal collision conditions have been characterized by employing mass spectrometry (collision induced dissociation), cryogenic photoelectron imaging spectroscopy, and quantum chemistry calculations. A theoretical analysis identified a crucial adsorption intermediate with N bonded with the V metal core in the end-on/side-on/side-on (ESS) mode, which most likely enables the facile cleavage of the N≡N bond. Such a vital N coordination in the ESS mode is a result of symmetry-matched interactions between the occupied orbitals of the metal core and both of the two empty π* orbitals of N. Furthermore, carbon ligands also play a considerable role in enhancing the reactivity of the metal core toward N. This study strongly suggests a new mechanism of N≡N bond cleavage by gas-phase metal clusters.
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http://dx.doi.org/10.1021/jacs.0c02021DOI Listing
June 2020

Diffusion kurtosis imaging in the prediction of poor responses of locally advanced gastric cancer to neoadjuvant chemotherapy.

Eur J Radiol 2020 Jul 23;128:108974. Epub 2020 Apr 23.

Department of Gastrointestinal Cancer Center Surgery, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital & Institute, No. 52 Fu-Cheng Road, Hai-Dian District, Beijing 100142, China. Electronic address:

Purpose: To assess the efficacy of diffusion kurtosis imaging (DKI) in the prediction of the treatment response to neoadjuvant chemotherapy in patients with locally advanced gastric cancer (LAGC).

Methods: A total of 31 LAGC patients were enrolled in this prospective study. All patients underwent diffusion-weighted MRI examination (with b = 0, 200, 500, 800, 1000, 1500, 2000 s/mm, the subscript denotes the number of signal averages) before and after chemotherapy. DKI and mono-exponential (b = 0, 800 s/mm) models were built. Apparent diffusion coefficient (ADC), mean diffusivity (MD) and mean kurtosis (MK) of the LAGC tumors were measured. The absolute change values (ΔX) and percentage change values (%ΔX) of the above parameters post neoadjuvant chemotherapy (NACT) were calculated. The response was evaluated according to the pathological tumor regression grade scores (effective response group: TRG 0-2, poor response group: TRG 3). Mann-Whitney U test and receiver operating characteristic (ROC) curves were applicated for statistical analysis.

Results: There were 17 patients in the effective response group (ERG), and 14 patients in the poor response group (PRG). The MK and MK values in PRG were significantly higher than those in ERG [(0.671 ± 0.026) and (0.641 ± 0.019) vs. (0.584 ± 0.023) and (0.519 ± 0.018), p < 0.001]. ADC and MD in PRG were significantly lower than those in ERG (p = 0.005, p =0.001). Significant differences were also observed for % ΔMK, ΔMD and ΔMK between the two groups (p < 0.05). The area under the curve (AUC) for the prediction of PRG was highest for MK (AUC = 0.958, cutoff value = 0.614). The MK and MK had the highest sensitivity (91.70 %) and specificity (93.80 %) in the prediction of PRG, respectively.

Conclusion: Both DKI and ADC values show potential for the prediction of the PRG in LAGC patients. The DKI parameters, especially MK displayed the best performance.
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http://dx.doi.org/10.1016/j.ejrad.2020.108974DOI Listing
July 2020

Four-Point Computed Tomography Scores for Evaluation of Occult Peritoneal Metastasis in Patients with Gastric Cancer: A Region-to-Region Comparison with Staging Laparoscopy.

Ann Surg Oncol 2020 Apr 21;27(4):1103-1109. Epub 2020 Jan 21.

Department of Radiology, Peking University Cancer Hospital and Institute, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing, China.

Background: Preoperative diagnosis of peritoneal metastasis with gastric cancer remains challenging. This study explored the abnormal computed tomography (CT) signs of occult peritoneal metastasis (OPM) and evaluated it by region-to-region comparison using staging laparoscopy, from which a 4-point CT score system was developed.

Methods: Patients with advanced gastric cancer (stage cT ≥ 2M0) diagnosed by CT were enrolled in the study. Occult peritoneal metastasis detected during staging laparoscopy was compared with preoperative CT to investigate the presence of abnormal signs by a region-to-region comparison. A 4-point CT score system was developed to define the radiologic characteristics. Subsequently, the diagnostic efficacy of the CT score system was prospectively verified.

Results: In this study, 57 OPM regions were detected by staging laparoscopy in 33 of the 385 enrolled patients. The greater omentum was the most frequent site of OPM (38.60%, 22/57), which usually exhibited a smudge-like ground-glass opacity (S-GGO) (90.91%, 20/22) with a mean CT score of 2.14. The parietal and perihepatic peritoneum was the second most common site (22.81%, 13/57). A 4-point CT score system was developed based on the results. A cutoff CT score of 2 or higher was associated with a false-negative rate of 2% (2/99). This CT score system had a sensitivity of 87.5% and a specificity of 76.4% for an OPM-positive diagnosis (area under the curve, 0.848). The agreement between two radiologists on the assigned final score was 76.2% (kappa, 0.5).

Conclusions: Patients with OPM mostly exhibited S-GGO on CT, which should be interpreted cautiously. The 4-point CT score system may improve the pretreatment evaluation of occult peritoneal metastasis, and staging laparoscopy might not be necessary for patients with a score lower than 2.
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http://dx.doi.org/10.1245/s10434-019-07812-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060153PMC
April 2020

Safety and feasibility of laparoscopic spleen-preserving No. 10 lymph node dissection for locally advanced upper third gastric cancer: a prospective, multicenter clinical trial.

Surg Endosc 2020 11 10;34(11):5062-5073. Epub 2019 Dec 10.

Department of Gastric Surgery, Fujian Medical University Union Hospital, No. 29 Xinquan Road, Fuzhou, 350001, Fujian, China.

Background: Previous retrospective studies have shown that laparoscopic spleen-preserving D2 total gastrectomy (LSTG) for advanced upper third gastric cancer (AUTGC) is safe. However, all previous studies were underpowered. We therefore conducted a prospective, multicenter study to evaluate the technical safety and feasibility of LSTG for patients with AUTGC.

Methods: Patients diagnosed with AUTGC (cT2-4a, N-/+, M0) underwent LSTG at 19 institutions between September 2016 and October 2017 were included. The number of No. 10 lymph node (LN) dissections, metastasis rates, intraoperative and postoperative complications were investigated.

Results: A total of 251 patients were enrolled in the study, and 242 patients were eligible for the per protocol analysis. The average numbers of No. 10 LN dissections and metastases were 2.4 and 0.1, respectively. Eighteen patients (7.4%) had No. 10 LN metastases, and among patients with advanced gastric cancer, the rate of No. 10 LN metastasis was 8.1% (18/223). pN3 status was an independent risk factor for No. 10 LN metastasis. Intraoperative complications occurred in 7 patients, but no patients required conversion to open surgery or splenectomy. The overall postoperative complication rate was 13.6% (33/242). The major complication and mortality rates were 3.3% (8/242) and 0.4% (1/242), respectively. The number of retrieved No. 10 LNs, No. 10 LN metastasis and TNM stage had no significant influence on postoperative complication rates.

Conclusion: LSTG for AUTGC was safe and effective when performed by very experienced surgeons, this technique could be used in patients who needed splenic hilar lymph node dissection.
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http://dx.doi.org/10.1007/s00464-019-07306-8DOI Listing
November 2020

Side-on-End-on Coordination of Dinitrogen on a Polynuclear Vanadium Nitride Cluster Anion [V N ].

Chemistry 2019 Oct 21. Epub 2019 Oct 21.

State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (P. R. China), Beijing National Laboratory for Molecular Sciences and CAS Research, Education Centre of Excellence in Molecular Sciences, Beijing, 100190, P. R. China.

The side-on-end-on coordination of N can be very important to activate and functionalize this very stable molecule. However, such coordination has rarely been reported. This study reports a gas-phase species (a polynuclear vanadium nitride cluster anion [V N ] ) that can capture N efficiently (12 %), and the quantum chemistry modelling suggests an unusual side-on-end-on coordination. The cluster anions were generated by laser ablation and the reaction with N has been characterized by mass spectrometry, photoelectron imaging spectroscopy, and density functional theory calculations. The back-donation interactions between the localized d-d bonding orbitals on the low-coordinated dual metal (V) sites and the antibonding π* orbitals of N are the driving forces to adsorb N with a high binding energy (about 2.0 eV).
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http://dx.doi.org/10.1002/chem.201904362DOI Listing
October 2019

Direct Conversion of Methane with Carbon Dioxide Mediated by RhVO Cluster Anions.

Angew Chem Int Ed Engl 2019 Nov 22;58(48):17287-17292. Epub 2019 Oct 22.

State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.

Direct conversion of methane with carbon dioxide to value-added chemicals is attractive but extremely challenging because of the thermodynamic stability and kinetic inertness of both molecules. Herein, the first dinuclear cluster species, RhVO , has been designed to mediate the co-conversion of CH and CO to oxygenated products, CH OH and CH O, in the temperature range of 393-600 K. The resulting cluster ions RhVO CO after CH OH formation can further desorb the [CO] unit to regenerate the RhVO cluster, leading to the successful establishment of a catalytic cycle for methanol production from CH and CO (CH +CO →CH OH+CO). The exceptional activity of Rh-V dinuclear oxide cluster (RhVO ) identified herein provides a new mechanism for co-conversion of two very stable molecules CH and CO .
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http://dx.doi.org/10.1002/anie.201911195DOI Listing
November 2019

Dinitrogen Fixation and Reduction by TaNH Cluster Anions at Room Temperature: Hydrogen-Assisted Enhancement of Reactivity.

J Am Chem Soc 2019 Aug 31;141(32):12592-12600. Epub 2019 Jul 31.

Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, School of Chemistry and Chemical Engineering , Beijing Institute of Technology , 100081 Beijing , China.

Dinitrogen activation and reduction is one of the most challenging and important subjects in chemistry. Herein, we report the N binding and reduction at the well-defined TaNH and TaN gas-phase clusters by using mass spectrometry (MS), anion photoelectron spectroscopy (PES), and quantum-chemical calculations. The PES and calculation results show clear evidence that N can be adsorbed and completely activated by TaNH and TaN clusters, yielding to the products TaNH and TaN, but the reactivity of TaNH is five times higher than that of the dehydrogenated TaN clusters. The detailed mechanistic investigations further indicate that a dissociative mechanism dominates the N activation reactions mediated by TaNH and TaN; two and three Ta atoms are active sites and also electron donors for the N reduction, respectively. Although the hydrogen atom in TaNH is not directly involved in the reaction, its very presence modifies the charge distribution and the geometry of TaNH, which is crucial to increase the reactivity. The mechanisms revealed in this gas-phase study stress the fundamental rules for N activation and the important role of transition metals as active sites as well as the new significant role of metal hydride bonds in the process of N reduction, which provides molecular-level insights into the rational design of tantalum nitride-based catalysts for N fixation and activation or NH synthesis.
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http://dx.doi.org/10.1021/jacs.9b03168DOI Listing
August 2019

Size-Dependent Association of Cobalt Deuteride Cluster Anions CoD (n = 0-4) with Dinitrogen.

J Am Soc Mass Spectrom 2019 Oct 24;30(10):1956-1963. Epub 2019 Jun 24.

State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.

Dinitrogen (N) activation by metal hydride species is of fundamental interest and practical importance while the role of hydrogen in N activation is not well studied. Herein, the structures of CoD (n = 0-4) clusters and their reactions with N have been studied by using a combined experimental and computational approach. The mass spectrometry experiments identified that the CoD (n = 2-4) clusters could adsorb N while the CoD (n = 0 and 1) clusters were inert. The photoelectron imaging spectroscopy indicated that the electron detachment energies of CoD are smaller than those of CoD, which characterized that it is easier to transfer electrons from CoD than from CoD to activate N. The density functional theory calculations generally supported the experimental observations. Further analysis revealed that the H atoms in the CoH (n = 2-4) clusters generally result in higher energies of the Co 3d orbitals in comparison with the CoH (n = 0 and 1) systems. By forming chemical bonds with H atoms, the Co atoms of CoH are less negatively charged with respect to the naked Co system, which leads to higher N binding energies of CoHN than that of CoN.
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http://dx.doi.org/10.1007/s13361-019-02226-2DOI Listing
October 2019

Effectiveness of fibrin sealant as hemostatic technique in accelerating ESD-induced ulcer healing: a retrospective study.

Surg Endosc 2020 03 24;34(3):1191-1199. Epub 2019 Jun 24.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Gastrointestinal Surgery, Peking University Cancer Hospital and Institute, No. 52 Fu-Cheng Road, Haidian District, Beijing, 100142, China.

Objectives: Healing of gastric endoscopic submucosal dissection (ESD)-induced ulcer is critical for patient recovery. During ESD treatment, submucosal incisions are made with an electrosurgical knife to accomplish en bloc resections of superficial lesions. Nevertheless, excess electrocoagulation may decrease the blood supply of ESD-induced ulcer and delay the ulcer healing. The aim of this retrospective study was to evaluate the effectiveness of conservative electrocoagulation followed by porcine fibrin sealant (FS) as a wound microvessels-protective hemostatic technique in promoting the healing of ESD-induced ulcer.

Methods: A total of 332 patients with early gastric cancer (EGCs), or gastric precancerous lesion and gastric adenoma were retrospectively analyzed. Propensity score matching was used to compensate for the differences in age, gender, tumor location, resected specimen area, and pathology. One-month ulcer healing rates and delayed bleeding were compared between two matched groups (combined hemostats group and electrocautery group).

Results: A total of 115 matched pairs were created after propensity score matching. There was no difference in tumor location, specimen surface area, tumor differentiation and invasion depth between groups. The completed healing rate 1 month after ESD was 44.3% in combined hemostats group and 30.4% in electrocautery group (P = 0.004). There was no difference in delayed massive bleeding rate between two groups (P = 0.300). In addition, based on the multivariate regression analysis for ulcer healing rate, the use of FS (OR, 0.348, 95% CI 0.196 - 0.617, P = 0.000) and larger specimen size (OR, 2.640, 95% CI 2.015-3.458, P = 0.000) were associated with nonhealing ulcer 1 month after ESD.

Conclusion: Applying conservative electrocoagulation followed by porcine FS as a wound microvessels-protective hemostatic technique can promote ESD-induced ulcer healing without increasing delayed bleeding.
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http://dx.doi.org/10.1007/s00464-019-06872-1DOI Listing
March 2020

CT findings in diagnosis of gastric bare area invasion: potential prognostic factors for proximal gastric carcinoma.

Jpn J Radiol 2019 Jul 3;37(7):518-525. Epub 2019 Apr 3.

From Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiology, Peking University Cancer Hospital and Institute, No. 52 Fu Cheng Road, Hai Dian District, Beijing, 100142, China.

Purpose: To investigate the correlation between the preoperative CT findings in diagnosis of gastric bare area (GBA) invasion and the 3-year-overall survival (OS) of patients with proximal gastric carcinoma (PGC).

Methods: 108 consecutive patients with PGC confirmed by biopsy underwent MDCT scan prior to gastrectomy were enrolled retrospectively from Dec 2009 to Dec 2014. GBA invasion in PGC were evaluated by measuring the direct CT signs including transmural involvement and lymph nodes in the GBA. The indirect signs were also evaluated including the infiltration of the diaphragm, gastrophrenic ligament and perigastric fat. Kaplan-Meier estimates with log-rank test and Cox proportional hazard model were used for analysis.

Results: The two raters achieved excellent agreement. Univariate Kaplan-Meier estimates indicated that postoperative chemotherapy (p = 0.003), transmural involvement (p < 0.001), lymph nodes in the GBA (p = 0.015) and cT staging (p = 0.002) were associated with OS. Cox proportional hazard model indicated that the transmural involvement (HR = 8.194, 95% CI 2.15-31.266), diaphragm involvement (HR = 0.21, 95% CI 0.042-0.986), perigastric fat infiltration (HR = 0.125, 95% CI 0.018-0.885; HR = 0.02, 95% CI 0.001-0.264), and cT staging were independent prognostic factors for OS.

Conclusion: CT findings of GBA invasion in patients with PGC, not only the transmural involvement but also the indirect signs are independent prognostic factors potentially, which should be given more emphasis in future clinical practice.
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http://dx.doi.org/10.1007/s11604-019-00837-zDOI Listing
July 2019

C-N Coupling in N Fixation by the Ditantalum Carbide Cluster Anions TaC.

Inorg Chem 2019 Apr 1;58(8):4701-4705. Epub 2019 Apr 1.

State Key Laboratory for Structural Chemistry of Unstable and Stable Species , Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190 , P. R. China.

The construction of C-N bonds by the direct incorporation of dinitrogen (N) instead of ammonia (NH) into active species is particularly desirable but has been rarely reported. Herein, a ditantalum carbide cluster anion (TaC) capable of cleaving the N≡N bond and constructing a C-N bond under mild conditions has been identified using mass spectrometry, photoelectron imaging spectroscopy, and quantum-chemical calculations. The photoelectron spectrum of TaCN is remarkably different from that of TaC and matches the simulated spectrum of the TaCN species with an end-on-bonded CN unit. The formation of the C-N bond has also been supported by the CN fragment observed in the collision-induced dissociation of TaCN. The exceptional reactivity of TaC is ascribed to the low-valent metal center serving as an electron reservoir. This study provides a non-NH route to construct C-N bonds by incorporating N into carbide compounds to produce nitrogenous species.
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http://dx.doi.org/10.1021/acs.inorgchem.8b03502DOI Listing
April 2019
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