Publications by authors named "Zhuo Zhang"

659 Publications

Co-assembled gold nanorod@tripeptide core-shell nanospheres for aqueous Hg removal.

J Colloid Interface Sci 2021 Apr 21;599:436-442. Epub 2021 Apr 21.

Key Laboratory of Colloid and Interface Chemistry & Key Laboratory of Special Aggregated Materials (Shandong University), Ministry of Education, Jinan 250100, China. Electronic address:

The construction of peptide and metal nanoparticles hybrid is attractive to explore their synergistic properties and applications extensively. However, it remains a challenge to fabricate a well-defined and size-controllable short peptide/Au nanoparticles hybrid. Here, we report a facile and flexible co-assembly strategy for the construction of tripeptide coated Au nanorods (AuNRs). The tripeptide (Phe-Phe-Cys, FFC) grows via covalently crosslinking to form spheres. The size of the core@shell nanospheres can be controlled by modulating the amount or size of AuNRs. Especially, the concentration of AuNRs fixes the amount of seeds, which further affect the local concentration of FFC on the surface of AuNRs seed, leading to a narrower diameter of hybrid compared to FFC spheres. Moreover, owing to their synergistic effects, this hybrid exhibits a good adsorption capacity of Hg from aqueous solutions by electrostatic interaction and forming into Au/Hg amalgam. This strategy could be extended to the fabrication of other biomolecules and metal nanoparticle hybrids with diverse functions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.04.100DOI Listing
April 2021

MiR-205-5p suppresses angiogenesis in gastric cancer by downregulating the expression of VEGFA and FGF1.

Exp Cell Res 2021 May 3:112579. Epub 2021 May 3.

Department of General Surgery, Peking University First Hospital, Beijing 100034, China. Electronic address:

Anti-angiogenic therapy represents one of the most promising treatment modalities for human cancers. However, the response to antiangiogenic therapy in gastric cancer (GC) remains dismal. To help identify new strategies for antiangiogenic therapy in GC, we evaluated miR-205-5p expression in GC tissues from TCGA database and our hospital, and its functions in angiogenesis were explored in vitro and in vivo. We investigated miR-205-5p expression and microvessel densities (MVDs) in GC tissues and liver metastases from patients. The function and mechanisms of miR-205-5p were examined in human cell lines and in xenograft mouse models. Associations between miR-205-5p expression and clinical characteristics were analyzed using either Pearson's χ test or Fisher's exact test. Differences in overall survival (OS) distributions were evaluated using the log-rank test. Differences in measurement data were compared using Student's t-test and one-way ANOVA. We found that miR-205-5p expression was downregulated in GC tissues and was negatively correlated with CD31 expression in both TCGA and our clinical samples. GC cell lines expressed low levels of miR-205-5p, and miR-205-5p upregulation significantly impaired the proliferation and angiogenesis of GC cells. Moreover, vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) expression and activation of extracellular-related kinase (ERK) signaling were suppressed by miR-205-5p. MiR-205-5p inhibition promoted malignant phenotypes by enhancing VEGFA and FGF1 expression, as well as the activation of ERK signaling. Angiogenesis and ERK signaling were decreased in response to VEGFA and FGF1 downregulation induced by miR-205-5p overexpression. The dual-luciferase reporter assay showed that VEGFA and FGF1 were direct targets of miR-205-5p. Xenograft mouse models revealed that miR-205-5p suppressed tumor growth by inhibiting neovascularization. Altogether, these results demonstrate that miR-205-5p suppresses angiogenesis in GC by attenuating the expression of VEGFA and FGF1, indicating that upregulation of miR-205-5p may represent as an antiangiogenic therapy for GC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.yexcr.2021.112579DOI Listing
May 2021

Case report: Secondary sclerosing cholangitis induced by lapatinib and vinorelbine in a metastasis breast cancer patient.

Thorac Cancer 2021 May 6. Epub 2021 May 6.

Department of Pharmacy, Peking University First Hospital, Beijing, China.

Secondary sclerosing cholangitis (SSC) is a rare cholestatic liver disease that may have a severe clinical course. A 61-year-old woman with a history of metastasis breast cancer was admitted to our hospital for the second cycle of chemotherapy with lapatinib and vinorelbine. The patient had no reports of elevated liver function tests (LFTs) in the previous multiple chemotherapies or history of liver disease. However, the admission laboratory results showed severe cholestatic liver injury with the possibility of SSC by magnetic resonance cholangiopancreatography. Although chemotherapy was discontinued and patient was treated with hepatoprotective drugs, the LFTs did not improve and liver biopsy indicated mild injury of intrahepatic bile duct epithelium and hepatocyte. We added ursodeoxycholic acid and prednisolone to protect the liver, and laboratory data showed a response. To prevent the progression, lapatinib and vinorelbine were reintroduced and transient increases in alanine aminotransferase and γ-glutamyl transpeptidase were observed. With no evidence of viral or autoimmune liver disease, SSC induced by lapatinib and vinorelbine was diagnosed. This is the first case report of tyrosine kinase inhibitors and vinorelbine induced SSC and clinicians should be aware of the possibility of it. More case reports about this adverse drug reaction are needed to delineate optimal management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1759-7714.13986DOI Listing
May 2021

Case Report: Repeated Low-Dose Rituximab Treatment Is Effective in Relapsing Neuro Behçet's Disease.

Front Neurol 2021 15;12:595984. Epub 2021 Apr 15.

Department of Neurology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

Neuro Behçet's disease (NBD) is a rare but most aggressive manifestation of Behçet's disease (BD) with a poor prognosis, and some patients even present a relapsing and treatment-resistant progressive course. In some relapsing NBD cases, traditional corticosteroids and immunosuppressive drugs show limited efficacy, while benefits of biological agents, such as anti-B-lymphocyte CD20 biological agent rituximab (RTX), gradually represent potential therapeutic advantages with clinical rapid remission and long-time maintenance. However, up to now, the optimal dosage of RTX in NBD is still elucidated. Here, we report two patients with relapsing NBD, despite continuous high dose steroids and sufficient azathioprine treatment, still presenting severe and relapsing meningoencephalitis or brainstem involvement. Repeated low-dose RTX (100 mg × 3/1 week apart, 100 mg repeated every 6 months) is then attempted with rapid recovery and sustained remission. The approach in our cases may expand therapeutic options and provide helpful references for relapsing NBD treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2021.595984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081882PMC
April 2021

Bioinspired organohydrogels with heterostructures: Fabrications, performances, and applications.

Adv Colloid Interface Sci 2021 Apr 9;292:102408. Epub 2021 Apr 9.

Key Laboratory of Colloid and Interface Chemistry & Key Laboratory of Special Functional Aggregated Materials (Shandong University), Ministry of Education, Jinan, Jinan 250100, China; State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China. Electronic address:

Since emerging in 1960, the artificial hydrogels have garnered enormous attentions in scientific community due to their high level of similarities to biological soft tissues in both structures and properties. With the proceeding of research, the concern of hydrogels is gradually shifted from fundamental investigation to abundant functionalization. In contrast to the natural soft tissues, the current artificial hydrogels still possess relatively simple structures and unsatisfactory environmental adaptability, extremely limiting their practical applications in complex environments. Enlightened by the prominent adaptability of biological organisms, the binary cooperative complementary principle is utilized to develop bioinspired organohydrogels by combining two components with opposite but cooperative physiochemical features. The present review provides the advanced progresses of bioinspired organohydrogels with sophisticated heterogeneous networks and desirably environmental adaptabilities. We clearly summarize the synthesizing strategies in regard to both corresponding mechanisms and typical examples, including macroscopic organohydrogels, organohydrogels with binary solvent, organohydrogels with heteronetworks, and emulsion-based organohydrogels. Meanwhile, the intriguing features of the reported organohydrogels, such as temperature resistance, switchable mechanics, adaptive wettability, and opposite components compatibility, are also clearly highlighted with a short overview of their promising applications. Ultimately, the current challenges and perspectives on the future development of bioinspired organohydrogels are also discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cis.2021.102408DOI Listing
April 2021

Design, Fabrication, and Testing of a Novel 3D 3-Fingered Electrothermal Microgripper with Multiple Degrees of Freedom.

Micromachines (Basel) 2021 Apr 15;12(4). Epub 2021 Apr 15.

Department of Mechanical and Production Engineering, Aarhus University, 8200 Aarhus, Denmark.

This paper presents the design, fabrication, and testing of a novel three-dimensional (3D) three-fingered electrothermal microgripper with multiple degrees of freedom (multi DOFs). Each finger of the microgripper is composed of a V-shaped electrothermal actuator providing one DOF, and a 3D U-shaped electrothermal actuator offering two DOFs in the plane perpendicular to the movement of the V-shaped actuator. As a result, each finger possesses 3D mobilities with three DOFs. Each beam of the actuators is heated externally with the polyimide film. The durability of the polyimide film is tested under different voltages. The static and dynamic properties of the finger are also tested. Experiments show that not only can the microgripper pick and place microobjects, such as micro balls and even highly deformable zebrafish embryos, but can also rotate them in 3D space.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/mi12040444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071525PMC
April 2021

p62 functions as a signal hub in metal carcinogenesis.

Semin Cancer Biol 2021 Apr 22. Epub 2021 Apr 22.

Department of Environmental Medicine, NYU School of Medicine, 341 East 25th Street, New York, NY 10010, USA. Electronic address:

A number of metals are toxic and carcinogenic to humans. Reactive oxygen species (ROS) play an important role in metal carcinogenesis. Oxidative stress acts as the converging point among various stressors with ROS being the main intracellular signal transducer. In metal-transformed cells, persistent expression of p62 and erythroid 2-related factor 2 (Nrf2) result in apoptosis resistance, angiogenesis, inflammatory microenvironment, and metabolic reprogramming, contributing to overall mechanism of metal carcinogenesis. Autophagy, a conserved intracellular process, maintains cellular homeostasis by facilitating the turnover of protein aggregates, cellular debris, and damaged organelles. In addition to being a substrate of autophagy, p62 is also a crucial molecule in a myriad of cellular functions and in molecular events, which include oxidative stress, inflammation, apoptosis, cell proliferation, metabolic reprogramming, that modulate cell survival and tumor growth. The multiple functions of p62 are appreciated by its ability to interact with several key components involved in various oncogenic pathways. This review summarizes the current knowledge and progress in studies of p62 and metal carcinogenesis with emphasis on oncogenic pathways related to oxidative stress, inflammation, apoptosis, and metabolic reprogramming.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.semcancer.2021.04.014DOI Listing
April 2021

Nanoemulsion fluorescent inks for anti-counterfeiting encryption with dual-mode, full-color, and long-term stability.

Chem Commun (Camb) 2021 Apr 23. Epub 2021 Apr 23.

Key Laboratory of Colloid and Interface Chemistry (Ministry of Education), Shandong University, Jinan 250100, China.

An oil-in-water nanoemulsion (O/W NE) is selected as the carrier to encapsulate hydrophobic dual-mode luminescent upconversion nanoparticles (UC NPs) and downconversion (DC) carbon quantum dots (CQDs) inside the oil droplets for forming water-based fluorescent inks. The NE inks conform well to the requirements of inkjet printing for anti-counterfeiting encryption applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cc00555cDOI Listing
April 2021

Effects of Smoking, and Drinking on Serum Gamma-Glutamyl Transferase Levels Using Physical Examination Data: A Cross-Sectional Study in Northwest China.

Int J Gen Med 2021 15;14:1301-1309. Epub 2021 Apr 15.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau, People's Republic of China.

Purpose: Although drinking and smoking have been associated with gamma-glutamyl transferase (GGT) levels, studies on the effects of smoking and drinking on GGT levels are scarce. The objective of this study was to assess the individual and combined effects of smoking, drinking on GGT levels in the Shaanxi province of Northwest China.

Participants And Methods: A questionnaire survey was conducted in a population that underwent health examination at the First Affiliated Hospital of Xi'an Jiaotong University and included employees of enterprises or public institutions. The survey was used to collect the baseline characteristics, smoking status, and drinking status of the participants. This information was collected from January 2019 to December 2019. Data related to the physical examinations were exported using the hospital information system (HIS). A linear regression model was employed to explore the effects of smoking and drinking on GGT levels. The restricted cubic spline model was applied to assess the dose-response relationship between amount of smoking, alcohol consumption and GGT levels.

Results: A total of 10,177 participants were included in the study. Linear regression indicated that smoking (β=3.37, 95% CI: 2.57-4.17) and drinking (β=5.55 L, 95% CI: 4.40-6.71) individually, and collectively (β=9.30, 95% CI: 7.83-10.76) had a positive effect on GGT levels. The restricted cubic spline presented a linear dose-response relationship between the amount of daily smoking and GGT levels (P for non-linearity=0.148, P for overall association <0.001, OR=2.49, 95% CI: 1.27-4.90), and that between weekly alcohol consumption and GGT levels (P for non-linearity=0.231, P for overall association <0.001, OR=4.79, 95% CI: 1.72-13.32). In case of females, stratified analysis showed that in comparison to the reference group, only current drinkers had a significant effect on GGT levels (OR=3.37, 95% CI: 0.19-6.55).

Conclusion: Smoking and drinking have a dose-dependent and a synergistic effect on GGT levels. They should be controlled concurrently, especially among males.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJGM.S301900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055286PMC
April 2021

Factor structure and construct validity of the short form of managing the emotions of others (MEOS-SF) scale in the Chinese sample.

PLoS One 2021 15;16(4):e0249774. Epub 2021 Apr 15.

The Department of Psychology, Renmin University of China, Beijing, China.

Objectives: Emotional manipulation is an important strategy in social interaction. The English version of MEOS-SF has been developed to make the measurement of such manipulation ability more efficient. The purpose of the current study was to assess the psychometric properties of the Chinese version of MEOS-SF.

Methods: Explore factor analysis and Confirmatory factor analysis were adopted to examine the Chinese version of the MEOS-SF factor structure in 645 Chinese participants (mean age = 24.68 ± 6.01 years) recruited online.

Results: Factor analysis supported a new three-factor model that included Conceal, Prosocial, and Non-prosocial, different from the original English MEOS-SF. Enhance and Divert merged to Prosocial factor while Worsen and Inauthentic merged to Non-prosocial factor because both prosocial and non-prosocial pairs had similar objectives, which would be perceived as the same thing by people in Eastern culture. As expected, MEOS-SF factors were found to be correlated with the Big Five, psychopathy, narcissism, Machiavellianism, and trait EI.

Conclusions: Our results suggested that the Chinese version of MEOS-SF had acceptable psychometric properties and could be used to assess emotional manipulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249774PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049282PMC
April 2021

Paeoniflorin protects PC12 cells from oxygen-glucose deprivation/reoxygenation-induced injury via activating JAK2/STAT3 signaling.

Exp Ther Med 2021 Jun 29;21(6):572. Epub 2021 Mar 29.

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.

Ischemic stroke is the most common type of stroke, and it has become a major health issue as it is characterized by high mortality and morbidity rates. Paeoniflorin (PF) is a natural compound and the main active ingredient of Radix Paeoniae. The aim of the present study was to investigate the role of PF in oxygen-glucose deprivation/reoxygenation (OGD/R)-induced injury of PC12 cells and its association with the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway. An model of OGD/R injury was established in PC12 cells. Subsequently, Cell Counting Kit-8 assay and ELISA were used to evaluate cell viability and the secretion of inflammatory factors, respectively, in PC12 cells subjected to OGD/R and treated with PF. The levels of oxidative stress indicators and inflammatory factors were measured using corresponding commercial kits. In addition, the apoptosis rate of PC12 cells subjected to OGD/R and treated with PF was determined by flow cytometry, and the expression of apoptosis-related proteins was analyzed by western blotting. Additionally, the expression levels of JAK2/STAT3 pathway-related proteins were also evaluated. The cell viability, levels of oxidative stress, inflammation and apoptosis were also measured in OGD/R-induced PC12 cell injury models following co-treatment of cells with PF and FLLL32, a specific inhibitor of JAK2/STAT3 signaling. Cell viability was reduced, while oxidative stress and inflammation were increased after OGD/R-induced injury. However, the treatment of cells with PF significantly enhanced cell viability, and alleviated oxidative stress, inflammation and apoptosis of OGD/R-treated PC12 cells. Furthermore, PF activated the JAK2/STAT3 signaling pathway. Following FLLL32 intervention, the effects of PF on oxidative stress, inflammation and apoptosis of OGD/R-treated PC12 cells were reversed. In conclusion, the findings of the present study suggested that PF may protect PC12 cells from OGD/R-induced injury via activating the JAK2/STAT3 signaling pathway, thus providing novel insight into the mechanism through which PF may alleviate ischemic stroke and indicating a potential strategy for ischemic stroke treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/etm.2021.10004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027733PMC
June 2021

The influence of TCM constitutions and neurocognitive function in elderly Macau individuals.

Chin Med 2021 Apr 13;16(1):32. Epub 2021 Apr 13.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China.

Background: Traditional Chinese medicine (TCM) constitution contributes to predicating disease occurrence and pathological progress. In this study, we investigate the correlation between TCM constitution and neurocognitive function in elderly Macau individuals.

Methods: A total of 313 older adults from elderly healthcare centers were recruited at random. The data of gender, age, education, sleeping hours, physical activities were collected, and the Geriatric Depression Scale, Hong Kong version of the Montreal Cognitive Assessment (MoCA) and categories of TCM constitution were administered.

Results: Of the 313 elderly individuals enrolled in this study, 86 (27.48%) were of balanced constitution. Among the other categories of TCM constitution, the most was Yin-deficiency (23.32%), followed by 53 (16.93%) with Phlegm-dampness. The average neurocognitive score of all elderly individuals was 18.01 ± 6.25. After adjusting for all possible confounds, multiple linear regression analysis showed that Qi-depressed constitution and neurocognitive scores were negatively correlated (β = - 2.66, 95%CI - 4.99 ~ - 0.33), Meanwhile, Yin-deficient constitution and neurocognitive scores were negatively correlated (β = - 2.10, 95%CI - 3.73 ~ - 0.47). Compared with balanced constitution, Qi-depressed constitution mainly affected visual-spatial ability dimension (β = - 0.91, 95%CI - 1.54 ~ - 0.28) and naming dimension (β = - 0.64, 95%CI - 1.04 ~ - 0.25), Yin-deficient constitution mainly affected visual space dimension (β = - 0.53, 95%CI - 0.97 ~ - 0.08).

Conclusion: Qi-depressed and Yin-deficient constitutions are associated with and contributed to the decline of neurocognitive function in senior adults, especially visual-spatial ability and naming dimensions. Further investigations into how TCM constitutions interact with neurocognitive function are needed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13020-021-00441-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045257PMC
April 2021

Isorhapontigenin alleviates lipopolysaccharide-induced acute lung injury via modulating Nrf2 signaling.

Respir Physiol Neurobiol 2021 Mar 30;289:103667. Epub 2021 Mar 30.

Department of Respiratory and Critical Care, Tianjin Medical University General Hospital, Tianjin, 300052, China. Electronic address:

Nuclear factor erythroid-2 related factor 2 (Nrf2) is involved in mitigating various oxidative stress- and inflammation-induced diseases, including acute lung injury/acute respiratory distress syndrome (ALI/ARDS). Isorhapontigenin (ISO), from the Chinese herb Gnetum cleistostachyum, exhibits antioxidant and anti-inflammatory properties. In this study, we explored the protective effects of ISO in ALI and its underlying molecular mechanisms. ISO significantly mitigated ALI by reducing the lung wet/dry weight ratio, protein concentration in the bronchoalveolar lavage fluid (BALF), and the levels of myeloperoxidase and malondialdehyde. ISO also improved the superoxide dismutase and glutathione activity in vivo. Moreover, ISO effectively ameliorated the changes in IL-1β, IL-6, and TNF-α concentrations in BALF, prevented IκB degradation, and inhibited the phosphorylation of NF-κB p65 subunit in lung tissues; furthermore, it enhanced the nuclear translocation of Nrf2 and inhibited IL-1β, IL-6, TNF-α, iNOS, COX-2, and ROS production in lipopolysaccharide-treated RAW264.7 cells. The protective effects of ISO in ALI were significantly reversed in ML385-treated RAW264.7 cells and the mouse model, indicating its role in Nrf2-activation. In conclusion, ISO effectively ameliorated lipopolysaccharide-induced ALI by reducing inflammation and oxidative stress, primarily through activation of Nrf2 signaling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.resp.2021.103667DOI Listing
March 2021

Assessment of CPS + EG, Neo-Bioscore and Modified Neo-Bioscore in Breast Cancer Patients Treated With Preoperative Systemic Therapy: A Multicenter Cohort Study.

Front Oncol 2021 16;11:606477. Epub 2021 Mar 16.

Institute of Mental Health, Peking University, Beijing, China.

This study was to assess the prognosis stratification of the clinical-pathologic staging system incorporating estrogen receptor (ER)-negative disease, the nuclear grade 3 tumor pathology (CPS + EG), Neo-Bioscore, and a modified Neo-Bioscore system in breast cancer patients after preoperative systemic therapy (PST). A retrospective multicenter cohort study was conducted from 12 participating hospitals' databases from 2006 to 2015. Five-year disease free survival (DFS), disease specific survival (DSS), and overall survival (OS) were calculated using Kaplan-Meier Method. Area under the curve (AUC) of the three staging systems was compared. Wald test and maximum likelihood estimates in Cox proportional hazards model were used for multivariate analysis. A total of 1,077 patients were enrolled. The CPS + EG, Neo-Bioscore, and modified Neo-Bioscore could all stratify the DFS, DSS, and OS (all P < 0.001). While in the same stratum of Neo-Bioscore scores 2 and 3, the HER2-positive patients without trastuzumab therapy had much poorer DSS (P = 0.013 and P values < 0.01, respectively) as compared to HER2-positive patients with trastuzumab therapy and HER2-negative patients. Only the modified Neo-Bioscore had a significantly higher stratification of 5-year DSS than PS (AUC 0.79 . 0.65, P = 0.03). So, the modified Neo-Bioscore could circumvent the limitation of CPS + EG or Neo-Bioscore.

Clinical Trial Registration: ClinicalTrials.gov, identifier NCT03437837.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.606477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009183PMC
March 2021

Neuro-Behcet disease.

Arch Med Sci 2021 26;17(2):569-573. Epub 2021 Feb 26.

Department of Neurology, The Second Hospital of Hebei Medical University, Rizhao Central Hospital, Shijiazhuang, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5114/aoms/132471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959042PMC
February 2021

Pretending to Be Better Than They Are? Emotional Manipulation in Imprisoned Fraudsters.

Front Psychol 2021 5;12:562269. Epub 2021 Mar 5.

School of Sociology, China University of Political Science and Law, Beijing, China.

Fraud can cause severe financial losses and affect the physical and mental health of victims. This study aimed to explore the manipulative characteristics of fraudsters and their relationship with other psychological variables. Thirty-four fraudsters were selected from a medium-security prison in China, and thirty-one healthy participants were recruited online. Both groups completed an emotional face-recognition task and self-report measures assaying emotional manipulation, psychopathy, emotion recognition, and empathy. Results showed that imprisoned fraudsters had higher accuracy in identifying fear and surprise faces but lower accuracy in identifying happiness than controls ( = 5.26, < 0.001; = 2.38, < 0.05; = 3.75, < 0.001). Significantly lower scores on non-prosocial factors on the Managing the Emotions of Others scale (MEOS) were found for imprisoned fraudsters, relative to controls ( = 3.21, < 0.01). Imprisoned fraudsters had low scores in the assessment of psychopathy than the control group, especially Factor 1 ( = 2.04, = 0.05). For empathy, imprisoned fraudsters had significantly higher scores in perspective-taking than controls ( = 2.03, = 0.05). Correlation analyses revealed that psychopathic traits were positively correlated with non-prosocial factors in both groups. However, the relationships between emotional manipulation and emotional recognition and empathy were not consistent across the groups. The results suggest that fraudsters may pretend to be as prosocial as healthy controls, who had lower antisocial tendencies, normal empathy ability, and would like to manipulate others' emotions positively during social interaction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2021.562269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973104PMC
March 2021

Exploration of Intrinsic Microbial Community Modulators in the Rice Endosphere Indicates a Key Role of Distinct Bacterial Taxa Across Different Cultivars.

Front Microbiol 2021 16;12:629852. Epub 2021 Feb 16.

Institute of Environmental Biotechnology, Graz University of Technology, Graz, Austria.

Microbial communities associated with the plant phyllosphere and endosphere can have both beneficial as well as detrimental effects on their hosts. There is an ongoing debate to which extend the phyllosphere and endosphere microbiome assembly is controlled by the host plant how pronounced cultivar effects are. We investigated the bacterial and fungal communities from the phyllosphere and endosphere of 10 different rice cultivars grown under identical environmental conditions in the frame of a targeted approach to identify drivers of community assembly. The results indicated that the endophytic bacterial communities were clearly separated into two groups. The α-diversity and microbial network complexity within Group I were significantly lower than in Group II. Moreover, the genera , , and were significantly more abundant in Group II and only present in the interaction networks of this group. These three genera were significantly correlated with α- and β-diversity of the endophytic bacterial community and thus identified as major drivers of the endosphere community. We have identified keystone taxa that shape endophytic bacterial communities of different rice cultivars. Our overall findings provide new insights into plant-microbe interactions, and may contribute to targeted improvements of rice varieties in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.629852DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920960PMC
February 2021

Cyanidin-3-glucoside Regulates Osteoblast Differentiation via the ERK1/2 Signaling Pathway.

ACS Omega 2021 Feb 11;6(7):4759-4766. Epub 2021 Feb 11.

School of Public Health, Shenyang Medical College, 146 North Huanghe Street, Shenyang, Liaoning 110034, China.

Osteoporosis, characterized by a gradual decrease in the number of osteoblasts and a gradual increase in bone resorption of osteoclasts in bone tissue, is a global chronic disease, which severely impairs the quality of life of the elderly. Therefore, it is extremely urgent to study the prevention and treatment of osteoporosis. It has been reported that anthocyanins can regulate bone metabolism and prevent osteoporosis. Cyanidin-3--glucoside (C3G), the most common type of anthocyanin in nature, widely exists in a variety of vegetables and fruits. Although it has been shown that C3G has multiple effects on osteoclasts, its impact(s) and underlying mechanism(s) on osteoblasts are still not clear. Here, we evaluated the effect of C3G on cell proliferation and differentiation of osteoblasts (extracted from the hip joint of patients with osteoporosis) and MC3T3-E1 (a kind of osteoblast cell line from mice). We also test the ability of osteoblasts to mineralize after C3G treatment. To find the underlying mechanism of the above effects, we further evaluated the role of the ERK signaling pathway in C3G regulation of osteoblasts. The results showed that C3G treatment enhanced osteoblast proliferation rate, osteoblast mineralization points, the mRNA levels and protein expression levels of OC (osteocalcin), and the level of ERK phosphorylation, which could be blocked by pretreatment with ERK signaling pathway inhibitor. The above results not only indicate that the ERK pathway was involved in C3G regulation of osteoblast differentiation but also provide strong suggestive evidence that osteoblasts may be promising targets in preventive and therapeutic strategies for osteoporosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c05603DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905819PMC
February 2021

Introduction of Colonic and Fecal Microbiota From an Adult Pig Differently Affects the Growth, Gut Health, Intestinal Microbiota and Blood Metabolome of Newborn Piglets.

Front Microbiol 2021 4;12:623673. Epub 2021 Feb 4.

Chongqing Academy of Animal Science, Chongqing, China.

Microbiota transplantation is a rapid and effective method for changing and reshaping the intestinal microbiota and metabolic profile in humans and animals. This study compared the different influences of the introduction of fecal microbes and colonic microbes from a fat, adult pig in newborn pigs. Both colonic microbiota transplantation (CMT) and fecal microbiota transplantation (FMT) promoted growth and improved gut functions in suckling pigs up to weaning. FMT was more beneficial for body weight gain and body fat deposition in piglets, while CMT was more beneficial for intestinal health and mucosal immunity. 16S rDNA sequence analysis indicated that both CMT and FMT significantly increased the abundances of beneficial or functional bacteria, such as and genera, in the piglets, and reduced the abundances of harmful bacteria, such as . Blood metabolome analysis showed that transplantation, especially FMT, enhanced lipid metabolism in piglets. In addition, while CMT also changed amino acid metabolism and increased anti-inflammatory metabolites such as 3-indoleacetic acid and 3-indolepropionic acid in piglets, FMT did not. Of note, FMT damaged the intestinal barrier of piglets to a certain extent and increased the levels of inflammatory factors in the blood that are potentially harmful to the health of pigs. Taken together, these results suggested that intestinal and fecal microbiota transplantations elicited similar but different physiological effects on young animals, so the application of microbiota transplantation in animal production requires the careful selection and evaluation of source bacteria.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.623673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889522PMC
February 2021

Platelet-rich plasma injection vs corticosteroid injection for conservative treatment of rotator cuff lesions: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Feb;100(7):e24680

Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, China.

Background: To explore the effectiveness of platelet-rich plasma (PRP) injection regarding functional recovery, pain relief, and range of motion (ROM) of shoulder compared with the corticosteroid injection in patients with rotator cuff lesions treated non-operatively.

Methods: An electronic literature search was performed by 2 authors in the PubMed, Embase, Cochrane Library, and Web of Science databases to identify relevant randomized controlled trial (RCTs) that were published up to July 20, 2020. The quality of the included RCTs was evaluated using the approach recommended by the Cochrane Handbook for Systematic Reviews of Interventions. Standardized mean differences (SMDs) or mean differences (MDs) with 95% confidence intervals (CIs) were applied to calculate the pooled effect sizes.

Results: Six RCTs were included in this systematic review. Meta-analysis revealed that corticosteroid injection yielded statistically significant superior functional recovery (SMD = -0.80; 95% CI, -1.42 to -0.18; P = .01) and pain relief (MD = 1.59; 95% CI, 0.30-2.89; P = .02) compared with PRP injection for rotator cuff lesions during the short-term follow-up period. However, at the medium-term and long-term follow-up, no statistically significant difference was identified between the 2 groups. Regarding the ROM of shoulder, no statistically significant difference was found between the 2 groups during the whole follow-up period.

Conclusions: The current clinical evidence revealed short-term efficacy of corticosteroid injection and no significant medium- to long-term difference between corticosteroid and PRP injection in the treatment of rotator cuff lesions. Additional studies with longer follow-ups, larger sample sizes, and more rigorous designs are needed to draw more reliable and accurate conclusions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000024680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7899870PMC
February 2021

Clinical and Polysomnographic Characteristics of Nonobese and Obese Chinese Patients With Obstructive Sleep Apnea.

J Clin Neurophysiol 2021 Feb 10. Epub 2021 Feb 10.

Department of Neurology, Xi'an Daxing Hospital, Xi'an City, Shaanxi Province, China; Sleep Disorders Center, Department of Neurology, Tangdu Hospital, Fourth Military Medical University, Xi'an City, Shaanxi Province, China; and Xi'an International Medical Center, Northwest University, Xi'an City, Shaanxi Province, China.

Purpose: Obesity is a risk factor associated with the onset and exacerbation of obstructive sleep apnea (OSA). However, the majority of OSA patients in Asian populations are nonobese. To date, there have been insufficient large-scale studies of the differences in the clinical and polysomnographic features of obese and nonobese OSA patients in this population, and few studies have sought to identify predictors of OSA severity in affected obese and nonobese patients.

Methods: We conducted a case-matched retrospective study, including 652 consecutive Chinese OSA patients (326 nonobese and 326 obese) to assess differences in demographic, clinical, and polysomnographic data between these two groups. Independent predictors of OSA severity were identified through multivariate linear regression analysis.

Results: The age and gender distributions of our obese and nonobese OSA patient cohorts did not differ significantly (P > 0.05), and rates of comorbidities were comparable in these two patient groups (P > 0.05). Nonobese patients were more likely to report atypical symptoms of OSA, including insomnia (P < 0.001), irritability (P < 0.05), and depressive symptoms (P < 0.01), whereas obese patients were more likely to report typical symptoms of OSA, such as habitual snoring (P < 0.001), witnessed apnea (P < 0.05), and daytime sleepiness (P < 0.001). Relative to nonobese patients, those who were obese exhibited significantly higher apnea-hypopnea index during total sleep time (P < 0.001), apnea-hypopnea index during nonrapid eye movement sleep (P < 0.001), and apnea-hypopnea index during rapid eye movement sleep (P < 0.001), in addition to lower average oxygen saturation (P < 0.001), minimal oxygen saturation (P < 0.001), and a higher oxygen desaturation index (P < 0.001) and arousal index (P < 0.001). Total sleep time was, on average, shorter for nonobese patients (P < 0.05), who also exhibited decreased sleep efficiency and more frequent awakening relative to obese patients (P < 0.05). A multivariate linear regression analysis revealed that neck circumference and waist circumference were independent predictors of OSA severity in obese patients (P < 0.05).

Conclusions: On average, OSA was typically less severe in nonobese patients, who were also more likely to experience atypical OSA symptoms relative to obese patients. These results also suggest that the differential contributions of body fat accumulation and distribution to OSA severity may offer insights into the pathogenesis, clinical manifestations, and optimal management of this condition in nonobese and obese patient populations. It is vital that clinicians consider these differences to properly diagnose and treat this debilitating condition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/WNP.0000000000000831DOI Listing
February 2021

Procyanidin B2 inhibits lipopolysaccharide‑induced apoptosis by suppressing the Bcl‑2/Bax and NF‑κB signalling pathways in human umbilical vein endothelial cells.

Mol Med Rep 2021 04 12;23(4). Epub 2021 Feb 12.

Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.

Human umbilical vein endothelial cells (HUVECs) serve a critical role in maintaining normal vascular function. Lipopolysaccharide (LPS), which is released from pathogenic bacteria in the blood, induces HUVEC apoptosis and injury to cause vascular dysfunction and infectious vascular diseases. Procyanidin B2 (PB2) possesses numerous functions, including antioxidant, antitumor, anti‑inflammatory and antiapoptosis effects, but the molecular mechanism is not completely understood. The present study investigated the effects of PB2 on LPS‑induced cytotoxicity and apoptosis in HUVECs, as well as the underlying mechanisms. The effects of PB2 on LPS‑mediated alterations to cytotoxicity, mitochondrial membrane potential, apoptosis were assessed by performing Cell Counting Kit‑8, JC‑1 fluorescence, Hoechst 33258 staining assays, respectively. IL‑1β, IL‑6 and TNF‑α mRNA expression and protein levels were measured by performing reverse transcription‑quantitative PCR and ELISAs, respectively. Bcl‑2, Bax, cleaved caspase‑3, cleaved caspase‑7, cleaved caspase‑9, phosphorylated (p)‑IκB‑α, p‑IκB‑β, p‑NF‑κB‑p65 and total NF‑κB p65 protein expression levels were determined via western blotting. NF‑κB p65 nuclear translocation was assessed via immunofluorescence. PB2 pretreatment markedly attenuated LPS‑induced cytotoxicity and apoptosis in HUVECs. PB2 also significantly downregulated the expression levels of IL‑1β, IL‑6, TNF‑α, Bax, cleaved caspase‑3, cleaved caspase‑7, cleaved caspase‑9 and p‑NF‑κB‑p65, but upregulated the expression levels of Bcl‑2, p‑IκB‑α and p‑IκB‑β in LPS‑induced HUVECs. Moreover, PB2 markedly inhibited LPS‑induced NF‑κB p65 nuclear translocation in HUVECs. The results suggested that the potential molecular mechanism underlying PB2 was associated with the Bax/Bcl‑2 and NF‑κB signalling pathways. Therefore, PB2 may serve as a useful therapeutic for infectious vascular diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2021.11906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893691PMC
April 2021

The Gene Can Regulate the Fecundity of the Green Peach Aphid, (Sulzer).

Front Physiol 2020 12;11:596392. Epub 2021 Jan 12.

College of Plant Protection, Hunan Agricultural University, Changsha, China.

(Sulzer), commonly known as the green peach aphid, is a notorious pest that causes substantial losses to a range of crops and can transmit several plant viruses, including potato virus Y (PVY). Chemical insecticides provide only partial control of this pest and their use is not environmentally sustainable. In recent years, many genes related to growth, development, and reproduction have been used as targets for pest control. These include (), a highly conserved gene that has been reported to play an essential part in the genesis of germline cells and, hence, in fecundity in the model insect . We hypothesized that the () gene was a potential target that could be used to regulate the population. In this study, we report the first investigation of an ortholog of in , designated , and describe its role in the fecundity of this insect. First, we identified the mRNA sequence in the transcriptome database, verified its identity with reverse transcription-polymerase chain reaction (RT-PCR), and then evaluated the transcription levels of in nymphs of different instars and tissues with real-time quantitative PCR (RT-qPCR). To investigate its role in regulating the fecundity of , we used RNA interference (RNAi) to silence the expression of in adult insects; this resulted in a significant reduction in the number of embryos (50.6%, < 0.01) and newborn nymphs (55.7%, < 0.01) in the treated aphids compared with controls. Interestingly, was also significantly downregulated in aphids fed on tobacco plants that had been pre-infected with PVY , concomitant with a significant reduction (34.1%, < 0.01) in fecundity. Collectively, these data highlight the important role of in regulating fecundity in and indicate that is a promising RNAi target gene for control of this pest species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphys.2020.596392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835840PMC
January 2021

Tomato Chlorosis Virus Infection Facilitates MED Reproduction by Elevating Expression.

Insects 2021 Jan 25;12(2). Epub 2021 Jan 25.

Department of Entomology, University of Kentucky, Lexington, KY 40546, USA.

Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase MED fecundity via elevated gene expression. As a result, fecundity and the relative expression of MED was measured on ToCV-infected and uninfected tomato plants on days 4, 8, 12, 16, 20 and 24. The role of on MED reproduction was examined in the presence and absence of ToCV using dietary RNAi. ToCV infection significantly increased MED fecundity on days 12, 16 and 20, and elevated expression on days 8, 12 and 16. Both ovarian development and fecundity of MED were suppressed when was silenced with or without ToCV infection. These combined results suggest that ToCV infection increases MED fecundity via elevated expression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/insects12020101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911321PMC
January 2021

A review of metal organic framework (MOFs)-based materials for antibiotics removal via adsorption and photocatalysis.

Chemosphere 2021 Jun 6;272:129501. Epub 2021 Jan 6.

School of Hydraulic Engineering, Changsha University of Science & Technology, Changsha, 410114, PR China.

Antibiotic abuse has led to serious water pollution and severe harm to human health; therefore, there is an urgent need for antibiotic removal from water sources. Adsorption and photodegradation are two ideal water treatment methods because they are cheap, simple to operate, and reusable. Metal organic frameworks (MOFs) are excellent adsorbents and photocatalysts because of their high porosity, adaptability, and good crystal form. The aim of this study is to suggest ways to overcome the limitations of adsorption and photocatalysis treatment methods by reviewing previous applications of MOFs to antibiotic adsorption and photocatalysis. The different factors influencing these processes are also discussed, as well as the various adsorption and photocatalysis mechanisms. This study provides a valuable resource for researchers intending to use MOFs to remove antibiotics from water bodies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2020.129501DOI Listing
June 2021

A comparative study of aurantio-obtusin metabolism in normal and liver-injured rats by ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry.

J Pharm Biomed Anal 2021 Mar 13;196:113896. Epub 2021 Jan 13.

College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100124, China; Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing, 100850, China. Electronic address:

Aurantio-obtusin, an anthraquinone isolated from cassiae semen, possesses diverse pharmacological activities, including hypotensive, hypolipidemic and anti-inflammatory effects. However, our previous studies demonstrated that exposure to aurantio-obtusin induced hepatotoxicity, but the mechanisms of the toxic effects remain unknown. The purpose of the present study is to establish a strategy for the metabolite profiling of aurantio-obtusin in normal and liver-injured rats. This study aimed at identifying the in vivo metabolites and the metabolic profiling in rats after oral administration at a dose of aurantio-obtusin (4 and 200 mg/kg) by using an ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) and metabolynx™ software. A total of 39 metabolites were detected and 3 of them were compared with standard substances. The results indicated that the principal metabolism pathways of aurantio-obtusin in normal rats were glucuronidation and sulfation, while in rats with liver injury, demethylation, dehydroxylation and reduction were also observed and regarded as new metabolic patterns of aurantio-obtusin. These findings helped us to understand the pharmacological and toxicological mechanisms of aurantio-obtusin. Moreover, this study could help to elucidate the metabolic profiling of other anthraquinones.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpba.2021.113896DOI Listing
March 2021

The complete mitochondrial genome of the (Passeriformes: Sittidae) from China.

Mitochondrial DNA B Resour 2020 Jun 5;5(3):2328-2329. Epub 2020 Jun 5.

College of Wildlife and Protected Area, Northeast Forestry University, Harbin, China.

We describe the whole mtDNA genome of the Chinese nuthatch in Tianlong Mountain, Shanxi, China. It is actually a circular molecular of 16,816 bp in length and consists 13 protein-coding genes, 22 transfer-RNA genes, 2 ribosomal-RNA genes, and 1 control region (D-loop, 1,243 bp in length). The nucleotide composition is 30.3% A, 30.1% C, 14.5% G, 25.1% T. The phylogenetic analysis based on the maximum likelihood method revealed the relationship of was close to the three reported species within the same genus , which are , and .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2020.1773341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782314PMC
June 2020

Identification of a Novel Bioactive Phenazine Derivative and Regulation of on Its Production in S015.

J Agric Food Chem 2021 Jan 14;69(3):974-981. Epub 2021 Jan 14.

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Natural phenazines are a class of multifunctional secondary metabolites of bacteria that play an important role in the biocontrol of plant pathogens. In this paper, a novel bioactive phenazine derivative was isolated from S015 through silica gel chromatography and preparative high-performance liquid chromatography (HPLC). The structure was identified as 1-carboxyl-6-formyl-4,7,9-trihydroxy-phenazine (CFTHP) by NMR spectroscopy in combination with ultraperformance liquid chromatography & mass spectrometry (UPLC-MS). CFTHP could inhibit , , , and f. sp. with minimal inhibitory concentration (MIC) values of 16, 32, 16, and 16 μg/mL, respectively. A global regulatory gene could positively regulate CFTHP biosynthesis since its production was 3.0-fold enhanced by overexpression and inhibited by deletion in S015. These studies illustrated the potential of CFTHP as a promising biopesticide and provided a reference for phenazine production improvement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.0c06498DOI Listing
January 2021

Mild cognitive impairment in novel SPG11 mutation-related sporadic hereditary spastic paraplegia with thin corpus callosum: case series.

BMC Neurol 2021 Jan 11;21(1):12. Epub 2021 Jan 11.

Department of Neurology, Tangdu Hospital, Fourth Military Medical University, Xi'an City, 710038, Shaanxi Province, China.

Background: SPG11 mutation-related autosomal recessive hereditary spastic paraplegia with thin corpus callosum (HSP-TCC) is the most common cause in complicated forms of HSP, usually presenting comprehensive mental retardation on early-onset stage preceding spastic paraplegias in childhood. However, there are many instances of sporadic late-onset HSP-TCC cases with a negative family history, and potential mild cognitive deficits in multiple domains may be easily neglected and inaccurately described.

Methods: In this study, we performed next generation sequencing in four sporadic late-onset patients with HSP-TCC, and combined Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) to evaluate cognition of the patients.

Results: By evolutionary conservation and structural modeling analysis, we have revealed 4 novel pathogenic SPG11 mutations, and firstly confirmed mild cognitive impairment (MCI) with normal MMSE scores (≥27) and decreased MoCA scores (< 26) in these SPG11 mutation-related HSP-TCC patients, predominantly presenting impairment of executive function, delayed recall, abstraction and language.

Conclusions: The results expand the mutational spectrum of SPG11-associated HSP-TCC from sporadic cases, and confirm MCI with combination of decreased MoCA and normal MMSE assessment, suggesting that clinicians should consider doing a MoCA to detect MCI in patients with HSP, particularly those with HSP-TCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12883-020-02040-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798194PMC
January 2021

Analysis of gamma-glutamyltransferase in acute promyelocytic leukemia patients undergoing arsenic trioxide treatment.

Hematology 2021 Dec;26(1):58-64

Department of Hematology, First Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China.

Objectives: The remarkable effect of arsenic trioxide (ATO) was verified, but elevated gamma-glutamyltransferase (GGT), aminotransferases (ALT and AST) are generally observed in acute promyelocytic leukemia (APL) patients undergoing ATO treatment. However, utilization of hepatoprotective agents or discontinuation of ATO may inhibit ATO efficacy. In order to maintain ATO effect from hepatoprotective agents' influence so we investigate relationships between single elevation in GGT and hepatocellular injury in this study.

Methods: Correlation of GGT variation and leukocyte counts were analyzed in all 81 APL patients, correlations among liver enzymes (ALT, AST and GGT) were also analyzed in patients without prophylactic hepatoprotective agents. In following study, we take the clinical observation of changes in aminotransferases in patients with single elevation in GGT without hepatoprotective agents.

Results: The average elevated GGT in the WBC abnormal group was more than the normal group (53.86U/L vs. 31.03U/L,  = 0.008), a positive Pearson's correlation of GGT variation and changed leukocyte counts in patients without prophylactic hepatoprotective agents. There are no significant correlation between aminotransferases (ALT and AST) and GGT but correlation between ALT and AST was statistically significant ( = 0.649,  = 0.000). For APL patients with single elevation in GGT, ALT and AST levels were normal throughout the ATO treatment without hepatoprotective agents.

Conclusion: Single elevation in GGT without elevated aminotransferases can't be identified as hepatotoxicity, and the elevated levels of GGT are associated with increasing leukocyte counts. Continue single-agent ATO without prophylactic hepatoprotective agents is recommended in APL patients with single elevation in GGT, in order to maintain ATO effect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/16078454.2020.1868782DOI Listing
December 2021