Publications by authors named "Zhuo Liu"

426 Publications

Inflammatory bowel disease is associated with worse sexual function: a systematic review and meta-analysis.

Transl Androl Urol 2022 Jul;11(7):959-973

Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Several studies report that sexuality is often affected by inflammatory bowel diseases (IBD). The aim of this meta-analysis was to investigate the association between IBD and sexual function.

Methods: A literature search was conducted in PubMed, Web of Science, and EMBASE databases (up to September 1, 2020). Scores of sexual functions with a standard deviation and odds ratio (OR) or relative risk (RR) with a 95% CI were used to analysis the association between IBD and sexual function.

Results: Eleven studies with 7,018 male IBD cases and 1,803 female IBD cases were included in the meta-analysis. In male individuals, the pooled results revealed that IBD was significantly associated with impaired erectile function and poor sexual satisfaction (RR for erectile function =1.50, 95% CI: 1.22 to 1.84, P<0.0001; standard mean difference for sexual satisfaction =-0.24, 95% CI: -0.33 to -0.15, P<0.0001). And among female individuals, IBD had impact on most sub-domains of sexual function, except pains.

Conclusions: IBD is associated with worse sexual function. It has significant impact on erectile function and satisfaction for male individuals and has impact on most sub-domains of sexual function for female individuals.
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http://dx.doi.org/10.21037/tau-22-190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360519PMC
July 2022

Effects of the Combined Application of Trimethylated Chitosan and Carbodiimide on the Biostability and Antibacterial Activity of Dentin Collagen Matrix.

Polymers (Basel) 2022 Aug 3;14(15). Epub 2022 Aug 3.

Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

The structural integrity of a dentin matrix that has been demineralized by the clinical use of etchants or calcium-depleting endodontic irrigants, such as endodontic ethylenediaminetetraacetic acid (EDTA), is often deteriorated due to the collagenolytic activities of reactivated endogenous enzymes as well as the infiltration of extrinsic bacteria. Therefore, the biomodification of dentin collagen with improved stability and antibacterial activity holds great promise in conservative dentistry. The purpose of this study was to evaluate the effects of the combined application of trimethylated chitosan (TMC) and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) on the biostability and antibacterial activity of the demineralized dentin collagen matrix. The morphological changes in the collagen matrix were observed by scanning electron microscopy (SEM), the amount of TMC adsorbed on the collagen surface was detected by X-ray photoelectron spectroscopy, and the elastic modulus was measured by a three-point bending device. Dry weight loss and amino acid release were detected to evaluate its anti-collagenase degradation performance. The antibacterial performance was detected by confocal microscopy. The TMC-treated group had less collagen space and a more compact collagen arrangement, while the untreated group had a looser collagen arrangement. The combined application of TMC and EDC can increase the elastic modulus, reduce the loss of elastic modulus, and result in good antibacterial performance. The current study proved that a dentin collagen matrix biomodified by TMC and EDC showed improved biodegradation resistance and antibacterial activities.
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http://dx.doi.org/10.3390/polym14153166DOI Listing
August 2022

Patterns of Lymph Node Metastasis and Optimal Surgical Strategy in Small (≤20 mm) Gastroenteropancreatic Neuroendocrine Tumors.

Front Endocrinol (Lausanne) 2022 21;13:871830. Epub 2022 Jul 21.

Department of Colorectal Surgery, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, China.

Background: Regional lymph node metastasis (LNM) is crucial for planning additional lymphadenectomy, and is directly correlated with poor prognosis in gastroenteropancreatic neuroendocrine tumors (GEP-NETs). However, the patterns of LNM for small (≤20 mm) GEP-NETs remain unclear. This population-based study aimed at evaluating LNM patterns and identifying optimal surgical strategies from the standpoint of lymph node dissemination.

Methods: This retrospective cohort study retrieved data from the Surveillance, Epidemiology, and End Results (SEER) 18 registries database for 17,308 patients diagnosed as having localized well-differentiated GEP-NETs ≤ 20 mm between January 1, 2004, and December 31, 2017. The patterns of LNM were characterized in 6,622 patients who underwent extended resection for adequate lymph node harvest.

Results: Of 6,622 patients with localized small GEP-NETs in the current study, 2,380 (36%) presented with LNM after regional lymphadenectomy. Nodal involvement was observed in approximately 7.4%, 49.1%, 13.6%, 53.7%, 13.8%, 7.8%, and 15.4% of gastric (g-), small intestinal (si-), appendiceal (a-), colonic (c-), rectal (r-), non-functional pancreatic (nfp-), and functional pancreatic (fp-) NETs ≤ 20 mm. Patients with younger age, larger tumor size, and muscularis invasion were more likely to present with LNM. Additional lymphadenectomy conferred a significant survival advantage in NETs (≤10 mm: HR, 0.47; 95% CI, 0.33-0.66; < 0.001; 11-20 mm: HR, 0.54; 95% CI, 0.34-0.85; = 0.008) and fp-NETs ≤ 20 mm (HR, 0.08; 95% CI, 0.02-0.36; = 0.001), as well as g-NETs (HR, 0.39; 95% CI, 0.16-0.96; = 0.041) and c-NETs of 11-20 mm (HR, 0.07; 95% CI, 0.01-0.48; = 0.007). Survival benefits of additional lymphadenectomy were not found in a-NETs, r-NETs, and nfp-NETs with a small size.

Conclusions: Given the increased risk for nodal metastasis, primary tumor resection with regional lymphadenectomy is a potential optimal surgical strategy for si-NETs and fp-NETs ≤ 20 mm, as well as g-NETs and c-NETs of 11-20 mm. Local resection is an appropriate and reliable surgical approach for a-NETs, r-NETs, and nfp-NETs ≤ 20 mm.
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http://dx.doi.org/10.3389/fendo.2022.871830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350735PMC
August 2022

Spermatic cord anastomosing hemangioma mimicking a malignant inguinal tumor: A case report and literature review.

Front Surg 2022 22;9:930160. Epub 2022 Jul 22.

Department of Urology, Peking University Third Hospital, Beijing, China.

Background: Anastomosing hemangioma (AH) is a rare vascular tumor and occurs in various organs. It is difficult to distinguish AH from malignant tumors even through multimodal imaging examination. AH located in the inguinal region is even rare. We present the diagnosis and treatment of a patient with spermatic cord AH in detail and conduct a literature review.

Case Report: An 84-year-old Chinese man had swelling pain in his right scrotum. A hard and fixed mass was palpable in the right inguinal region. Preoperative radiological examination considered it a neurogenic or vascular tumor. Malignant soft tissue sarcoma could not be excluded. He underwent radical inguinal right orchiectomy under intraspinal anesthesia. The diagnosis of spermatic cord AH was confirmed by pathological examination. The patient recovered uneventfully and remained disease-free during an 18-month follow-up.

Conclusion: Spermatic cord AH is quite rare and could be misdiagnosed as a malignant tumor. Pathological evidence might be necessary. The optimal choice of treatment should be determined through a comprehensive assessment of both tumor and patient factors.
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http://dx.doi.org/10.3389/fsurg.2022.930160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354528PMC
July 2022

A Self-Powered Optogenetic System for Implantable Blood Glucose Control.

Research (Wash D C) 2022 16;2022:9864734. Epub 2022 Jun 16.

Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Biomedical Synthetic Biology Research Center, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Dongchuan Road 500, Shanghai 200241, China.

Diabetes treatment and rehabilitation are usually a lifetime process. Optogenetic engineered designer cell-therapy holds great promise in regulating blood glucose homeostasis. However, portable, sustainable, and long-term energy supplementation has previously presented a challenge for the use of optogenetic stimulation . Herein, we purpose a self-powered optogenetic system (SOS) for implantable blood glucose control. The SOS consists of a biocompatible far-red light (FRL) source, FRL-triggered transgene-expressing cells, a power management unit, and a flexible implantable piezoelectric nanogenerator (i-PENG) to supply long-term energy by converting biomechanical energy into electricity. Our results show that this system can harvest energy from body movement and power the FRL source, which then significantly enhanced production of a short variant of human glucagon-like peptide 1 (shGLP-1) and . Indeed, diabetic mice equipped with the SOS showed rapid restoration of blood glucose homeostasis, improved glucose, and insulin tolerance. Our results suggest that the SOS is sufficiently effective in self-powering the modulation of therapeutic outputs to control glucose homeostasis and, furthermore, present a new strategy for providing energy in optogenetic-based cell therapy.
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http://dx.doi.org/10.34133/2022/9864734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275083PMC
June 2022

Artificial tactile perception smart finger for material identification based on triboelectric sensing.

Sci Adv 2022 Aug 5;8(31):eabq2521. Epub 2022 Aug 5.

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 101400, China.

Tactile perception includes the direct response of tactile corpuscles to environmental stimuli and psychological parameters associated with brain recognition. To date, several artificial haptic-based sensing techniques can accurately measure physical stimuli. However, quantifying the psychological parameters of tactile perception to achieve texture and roughness identification remains challenging. Here, we developed a smart finger with surpassed human tactile perception, which enabled accurate identification of material type and roughness through the integration of triboelectric sensing and machine learning. In principle, as each material has different capabilities to gain or lose electrons, a unique triboelectric fingerprint output will be generated when the triboelectric sensor is in contact with the measured object. The construction of a triboelectric sensor array could further eliminate interference from the environment, and the accuracy rate of material identification was as high as 96.8%. The proposed smart finger provides the possibility to impart artificial tactile perception to manipulators or prosthetics.
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http://dx.doi.org/10.1126/sciadv.abq2521DOI Listing
August 2022

Present and Future: Crosstalks Between Polycystic Ovary Syndrome and Gut Metabolites Relating to Gut Microbiota.

Front Endocrinol (Lausanne) 2022 19;13:933110. Epub 2022 Jul 19.

Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Polycystic ovary syndrome (PCOS) is a common disease, affecting 8%-13% of the females of reproductive age, thereby compromising their fertility and long-term health. However, the pathogenesis of PCOS is still unclear. It is not only a reproductive endocrine disease, dominated by hyperandrogenemia, but also is accompanied by different degrees of metabolic abnormalities and insulin resistance. With a deeper understanding of its pathogenesis, more small metabolic molecules, such as bile acids, amino acids, and short-chain fatty acids, have been reported to be involved in the pathological process of PCOS. Recently, the critical role of gut microbiota in metabolism has been focused on. The gut microbiota-related metabolic pathways can significantly affect inflammation levels, insulin signaling, glucose metabolism, lipid metabolism, and hormonal secretions. Although the abnormalities in gut microbiota and metabolites might not be the initial factors of PCOS, they may have a significant role in the pathological process of PCOS. The dysbiosis of gut microbiota and disturbance of gut metabolites can affect the progression of PCOS. Meanwhile, PCOS itself can adversely affect the function of gut, thereby contributing to the aggravation of the disease. Inhibiting this vicious cycle might alleviate the symptoms of PCOS. However, the role of gut microbiota in PCOS has not been fully explored yet. This review aims to summarize the potential effects and modulative mechanisms of the gut metabolites on PCOS and suggests its potential intervention targets, thus providing more possible treatment options for PCOS in the future.
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http://dx.doi.org/10.3389/fendo.2022.933110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343597PMC
August 2022

A Consensus-Reaching Approach to the Evaluation of Product Design Alternatives with Multiple Preference Structures.

Comput Intell Neurosci 2021 10;2021:6992648. Epub 2021 Nov 10.

College of Art and Design, Shaanxi University of Science & Technology, Xi'an 710021, China.

With interdisciplinarity being an important characteristic of contemporary product design, the evaluation of design alternatives also involves multiple disciplines, and the evaluator group usually consists of evaluators from different fields and with obvious heterogeneous characteristics. To effectively satisfy the heterogeneous needs of evaluators and improve the credibility of evaluation results, the paper introduces a consensus-reaching approach that incorporates multiple preferences to the evaluation of product design alternatives. First, in order to obtain individual preference information, each evaluator is asked to evaluate all the design alternatives using a preference structure that he/she is familiar with. Second, we use a transfer function to uniform the evaluation information obtained from various preference structures into a complementary judgment matrix. Then, we use the Hybrid Weighted Averaging (HWA) operator weight determination model to aggregate the preference information and obtain the group preference information. Then, we measure the consensus degree between individual evaluators and the group using a consensus measurement method. After that, we use the feedback mechanism to instruct individual evaluators to modify their preferences until a consensus is achieved. We explain the application steps and the feasibility of this approach through the evaluation of the design alternatives of multichannel fluorescence immunochromatography analyzers (MFIAs).
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http://dx.doi.org/10.1155/2021/6992648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338745PMC
August 2022

Study on the Relationship between Lung Cancer Stromal Cells and Air Cavity Diffusion Based on an Image Acquisition System.

Contrast Media Mol Imaging 2022 14;2022:2492124. Epub 2022 Jul 14.

Department of Emergency, The Hospital of Xidian Group, No. 97 FengDeng Rd, Xian, China.

Objective: The study aimed to investigate the role of tumor stromal cells in the pathogenesis of STAS, the relationship between air diffusion (STAS) and tumor stromal cells (TSCs) was studied, and the prognostic significance of TSC and STAS in patients with lung adenocarcinoma was evaluated.

Methods: A total of 150 patients with lung cancer diagnosed in the Affiliated Hospital of Jiangsu Province were selected. From the perspective of pathology, medical information technology was used to assist the diagnosis. The data of multiple magnetic resonance images were analyzed by three-dimensional space convolution (CNN), fuzzy neural network (FNN), transfinite learning machine (ELM), and binarization.

Result: After data fusion, the specificity and sensitivity of multiple magnetic resonance (MRI) data are significantly higher than those of single MRI data, and the more fusion times, the better the sensitivity and specificity. With the increase in the number of information and data fusion, the proportion of the significant effect and the comprehensive effective rate of patients are on the rise. Multiple MRI data fusion examination and analysis under medical information technology can improve the cure rate of patients, and the 1-year survival rate and the 3-year survival rate of patients have also gradually improved.

Conclusion: The MRI data fusion diagnosis method under the application of information technology can improve the sensitivity and specificity of the diagnosis results and comprehensively improve the clinical cure rate and the survival rate at different times of prognosis. In the context of the current big data information age, this multifeature fusion analysis technology is playing a more and more important role in medical treatment. The application of this method and technology not only improves the quality of life of patients but also processes multiple types of data at one time only by using the proposed medical assistant diagnosis model, which can save the diagnosis time to a certain extent. It has effectively realized the medical management and medical service quality and has important promotion significance.
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http://dx.doi.org/10.1155/2022/2492124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303510PMC
August 2022

A Novel CaMKII Inhibitory Peptide Blocks Relapse to Morphine Seeking by Influencing Synaptic Plasticity in the Nucleus Accumbens Shell.

Brain Sci 2022 Jul 26;12(8). Epub 2022 Jul 26.

School of Life Sciences, Peking University, Beijing 100871, China.

Drugs of abuse cause enduring functional disorders in the brain reward circuits, leading to cravings and compulsive behavior. Although people may rehabilitate by detoxification, there is a high risk of relapse. Therefore, it is crucial to illuminate the mechanisms of relapse and explore the therapeutic strategies for prevention. In this research, by using an animal model of morphine self-administration in rats and a whole-cell patch-clamp in brain slices, we found changes in synaptic plasticity in the nucleus accumbens (NAc) shell were involved in the relapse to morphine-seeking behavior. Compared to the controls, the amplitude of long-term depression (LTD) induced in the medium spiny neurons increased after morphine self-administration was established, recovered after the behavior was extinguished, and increased again during the relapse induced by morphine priming. Intravenous injection of MA, a new peptide obtained by modifying Ca/calmodulin-dependent protein kinase II (CaMKII) inhibitor "myr-AIP", decreased CaMKII activity in the NAc shell and blocked the reinstatement of morphine-seeking behavior without influence on the locomotor activity. Moreover, LTD was absent in the NAc shell of the MA-pretreated rats, whereas it was robust in the saline controls in which morphine-seeking behavior was reinstated. These results indicate that CaMKII regulates morphine-seeking behavior through its involvement in the change of synaptic plasticity in the NAc shell during the relapse, and MA may be of great value in the clinical treatment of relapse to opioid seeking.
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http://dx.doi.org/10.3390/brainsci12080985DOI Listing
July 2022

Neural Processing Differences of Facial Emotions Between Human and Vehicles: Evidence From an Event-Related Potential Study.

Front Psychol 2022 7;13:876252. Epub 2022 Jul 7.

School of Art and Design, Tianjin University of Technology, Tianjin, China.

Vehicle "faces" are a crucial factor influencing consumer intention to purchase gasoline and electric vehicles. However, little empirical evidence has demonstrated whether people process a vehicle's face similarly to a human's face. We investigated the neural processing relationship among human facial emotions and facial emotions of gasoline and electric vehicles using a 2 (emotional) × 3 (face type) repeated measures design and electroencephalograph (EEG) recordings. The results showed that human faces appear to share a partly similar neural processing mechanism in the latency of 100-300 ms, and that both human and vehicle faces elicited the ERP components N170, EPN, and P2. The large EPN and P2 suggest that gasoline vehicle facial emotions can be perceived more efficiently than those of electric vehicles. These findings provide an insight for vehicle designers to better understand the facial emotions presented by cars.
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http://dx.doi.org/10.3389/fpsyg.2022.876252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302361PMC
July 2022

Occupational Stress and Insomnia Symptoms Among Nurses During the Outbreak of COVID-19 in China: The Chain Mediating Effect of Perceived Organizational Support and Psychological Capital.

Front Psychiatry 2022 7;13:882385. Epub 2022 Jul 7.

School of Nursing, The Fourth Military Medical University, Xi'an, China.

Background: Nurses play an important role in medical and health services and insomnia symptoms were relatively high among nurses, especially during the epidemic of 2019 coronavirus disease. Insomnia not only damages the physical and mental health of the individual, but also reduces the efficiency of their work and the quality of care, ultimately impacting on patient care.

Objective: The purpose of this study was to explore the role of perceived organizational support and psychological capital in the relationship between occupational stress and insomnia among Chinese nurses.

Methods: A cross-sectional study has been carried out in a tertiary grade A hospital in Shandong Province, China from March 2021 to May 2021. The self-administered questionnaires were distributed to 810 nurses, which including Chinese Effort-Reward Imbalance Scale, Athens Insomnia Scale, Perceived Organizational Support Questionnaire, Chinese Psychological Capital Questionnaire, gender, age, education level and other demographic characteristics. Effective respondents were 658 (81.2%). Descriptive analysis, independent-samples -test, one-way analysis of variance, Pearson correlation analyses, ordinary least-squares regression and the bootstrap method were used for data analysis.

Results: The prevalence of insomnia symptoms in this study was found to be 57.3%. There were significant differences in insomnia symptoms in weekly working hours ( = -2.027, = 0.043), with chronic disease ( = -2.825, = 0.005), negative life events ( = -5.340, < 0.001), departments ( = 3.077, = 0.006) and position ( = 2.322, = 0.021) among nurses. Overall, the serial-multiple mediations of perceived organizational support and psychological capital in the relationship between occupational stress and insomnia were found to be statistically significant.

Conclusions: The prevalence of insomnia symptoms was comparatively high among Chinese nurses, and occupational stress had direct negative influence on it. Perceived organizational support and psychological capital acted as chained mediating factor could partially relieve insomnia symptoms related to occupational stress. Supportive working environment should be provided, and improving psychological capital levels to help nurses coping with insomnia symptoms.
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http://dx.doi.org/10.3389/fpsyt.2022.882385DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300995PMC
July 2022

Liver kinase B1 in exosomes inhibits immune checkpoint programmed death ligand 1 and metastatic progression of intrahepatic cholangiocarcinoma.

Oncol Rep 2022 09 20;48(3). Epub 2022 Jul 20.

Third Department of General Surgery, The Central Hospital of Xiangtan, Xiangtan, Hunan 411100, P.R. China.

The increasing morbidity and high mortality of intrahepatic cholangiocarcinoma (ICC) has led to the urgent need for new diagnostics and therapeutics. Liver kinase B1 (LKB1) exerts a tumor suppressor role in multiple malignances, while its regulatory role in exosomes secreted by ICC cells is obscure. In the present study, exosomes were extracted from cell culture supernatants of RBE and HCCC‑9810 ICC cells as well as plasma of patients with ICC by ultracentrifugation and the morphology of exosomes was identified by transmission electron microscopy. Notably, compared with that of intracellular LKB1, the protein level of exosomal LKB1 was decreased. Silencing intracellular LKB1 increased the protein levels of programmed death ligand 1 (PD‑L1), Slug and phosphorylated‑AKT in exosomes, accompanied by decreased expression levels of exosomal LKB1. Exosomes with lower protein levels of LKB1 promoted the expression of the immune checkpoint PD‑L1, malignant phenotypes of ICC cells , and cancer metastasis . Moreover, the low level of exosomal LKB1 in plasma was tightly associated with the poor prognosis of patients with ICC. Collectively, exosomal LKB1 inhibits the immune checkpoint PD‑L1 and metastasis of ICC cells. These findings may provide new methods for the diagnosis and immune therapy of ICC.
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http://dx.doi.org/10.3892/or.2022.8367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350976PMC
September 2022

Supplementation With Zinc Proteinate Increases the Growth Performance by Reducing the Incidence of Diarrhea and Improving the Immune Function of Dairy Calves During the First Month of Life.

Front Vet Sci 2022 1;9:911330. Epub 2022 Jul 1.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.

Two experiments were conducted to identify the optimal dose of zinc proteinate (ZP) in the diet for dairy calves and then to compare early supplementation with the ZP or zinc methionine (ZM) on the growth performance, incidence of diarrhea, antioxidant status, and immune function of dairy calves during their first month of life. In Experiment 1, forty newborn female Holstein dairy calves were randomly divided into four groups ( = 10): a control group (without ZP supplementation, ZP0) or groups that received ZP supplementation at 40, 80, and 120 mg zinc/day, respectively (ZP40, ZP80, and ZP120). The experiment lasted 14 days, and the growth performance, incidence of diarrhea, and serum zinc concentration were analyzed. In Experiment 2, thirty-six newborn female Holstein dairy calves were randomly allocated to three groups ( = 12): a negative control group (without zinc supplementation, CON), a positive control group (supplemented with 80 mg zinc/day in the form of zinc methionine, ZM), and a ZP group (supplemented with 80 mg zinc/day in the form of ZP). The experiment lasted 28 days, and the growth performance, incidence of diarrhea, serum zinc concentration, serum antioxidant indicators, and concentrations of plasma immunoglobulins and cytokines were determined on days 7, 14, 21, and 28. Results showed that in Experiment 1, supplementation with ZP to yield 80 mg zinc/day increased the ADG ( < 0.01) and serum zinc concentration ( < 0.01), and decreased the F/G ( < 0.01) and the incidence of diarrhea ( < 0.05) during days 1-14. In Experiment 2, compared with the CON group, ZP increased the ADG ( < 0.01), serum zinc concentration ( < 0.01), and plasma immunoglobulin G (IgG; < 0.01) and IgM ( < 0.01) concentrations, but reduced the incidence of diarrhea ( < 0.01), serum malondialdehyde ( < 0.01), and plasma interleukin-1β ( < 0.01) concentrations during days 1-28. Overall, ZP supplementation to yield 80 mg zinc/day improves the growth performance and immune function, and decrease the incidence of diarrhea of dairy calves, which was comparable to the same dose of zinc in the form of ZM.
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http://dx.doi.org/10.3389/fvets.2022.911330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284037PMC
July 2022

High-quality ice plant reference genome analysis provides insights into genome evolution and allows exploration of genes involved in the transition from C3 to CAM pathways.

Plant Biotechnol J 2022 Jul 15. Epub 2022 Jul 15.

College of Life Sciences/Center for Genomics and Bio-computing, North China University of Science and Technology, Tangshan, Hebei, China.

Ice plant (Mesembryanthemum crystallinum), a member of the Aizoaceae family, is a typical halophyte crop and a model plant for studying the mechanism of transition from C3 photosynthesis to crassulacean acid metabolism (CAM). Here, we report a high-quality chromosome-level ice plant genome sequence. This 98.05% genome sequence is anchored to nine chromosomes, with a total length of 377.97 Mb and an N50 scaffold of 40.45 Mb. Almost half of the genome (48.04%) is composed of repetitive sequences, and 24 234 genes have been annotated. Subsequent to the ancient whole-genome triplication (WGT) that occurred in eudicots, there has been no recent whole-genome duplication (WGD) or WGT in ice plants. However, we detected a novel WGT event that occurred in the same order in Simmondsia chinensis, which was previously overlooked. Our findings revealed that ice plants have undergone chromosome rearrangements and gene removal during evolution. Combined with transcriptome and comparative genomic data and expression verification, we identified several key genes involved in the CAM pathway and constructed a comprehensive network. As the first genome of the Aizoaceae family to be released, this report will provide a rich data resource for comparative and functional genomic studies of Aizoaceae, especially for studies on salt tolerance and C3-to-CAM transitions to improve crop yield and resistance.
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http://dx.doi.org/10.1111/pbi.13892DOI Listing
July 2022

T.H.R.O.B.V.S. Score - A Comprehensive Model to Predict the Surgical Complexity of Renal Cell Carcinoma With Tumor Thrombus.

Front Oncol 2022 23;12:900550. Epub 2022 Jun 23.

Department of Urology, Peking University Third Hospital, Beijing, China.

Background: To propose a quantitative model for predicting the surgical complexity of patients with renal cell carcinoma (RCC) and venous tumor thrombus (VTT).

Method: The clinical data of 226 cases of RCC with VTT in Peking University Third Hospital from January 2014 to August 2020 were retrospectively analyzed. Seven indicators were selected to establish the T.H.R.O.B.V.S. system, including alkaline phosphatase, tumor thrombus height, maximum tumor diameter, obesity, bland thrombus, vascular wall invasion, and side. Each indicator was assigned with 0, (1), and 2 points, and the total scores of 0~2, 3~5, and ≥6 were set as the low-, middle-, and high-risk groups, respectively. The surgical complexity was compared and validated among groups.

Results: As the risk increased, the proportion of open surgery significantly increased (P<0.001). The operation time (P<0.001), intraoperative blood loss (P<0.001), blood or plasma transfusion (P<0.001), and hospitalization (P<0.001) increased significantly. The postoperative complications (P<0.001), including notable complications (≥Clavein-Dindo II, P<0.001), were significantly different, and similar trends were shown in the validation group.

Conclusion: The T.H.R.O.B.V.S. scoring system is a quantifiable and satisfactory model to predict the surgical complexity and perioperative management of RCC with VTT.
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http://dx.doi.org/10.3389/fonc.2022.900550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261335PMC
June 2022

Assessing the Influencing Factors of Electronic Word-of-Mouth on CSR Issues. A Case of Hospotality Service Industry of China.

Front Psychol 2022 21;13:924779. Epub 2022 Jun 21.

Department of Finance and Economics, College of Business, University of Jeddah, Jeddah, Saudi Arabia.

Corporate social responsibility (CSR) information can be effectively disseminated via social media in a variety of industries, including the hospitality sector. In the same way, the media has a significant impact on CSR because the news media helps companies achieve their CSR goals. Prior research has not examined the main factors that influence electronic word of mouth (eWOM) on media coverage of CSR issues via social networking websites. For the purpose of examining the most significant predictors of intention to share or comment on negative CSR news reported by one media outlet on a specific social networking site (SNS). 677 Wechat users in China were surveyed in order to test the proposed model empirically. According to the findings of the study, eWOM intentions are positively influenced by environmental CSR content, and advertisement related CSR content. It also confirmed that the value of information is positively influenced by the credibility of the source. The variables interpersonal influencer impact and privacy concerns had no significant relationship, nor did they have any significant relationship with the intentions to share and comment on Wechat. Further the study findings suggest the theoretical and managerial policy recommendation for decision makers.
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http://dx.doi.org/10.3389/fpsyg.2022.924779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256499PMC
June 2022

A Risk Model Based on Sorafenib-Response Target Genes Predicts the Prognosis of Patients with HCC.

J Oncol 2022 28;2022:7257738. Epub 2022 Jun 28.

Third Department of General Surgery, The Central Hospital of Xiangtan, Xiangtan, Hunan, China.

Sorafenib is used to treat digestive system tumors in patients who do not respond to or cannot tolerate surgery. However, the roles and inhibitory mechanisms of sorafenib against hepatocellular carcinoma (HCC) are unclear. Differentially expressed genes in tissues from responders and nonresponders to sorafenib were investigated using the HCC GSE109211 data set. Biological functions and mechanisms were studied using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. The expression levels of differential expressed target genes were identified in HCC tissues, using The Cancer Genome Atlas database, and their prognostic and diagnostic values were explored using survival and receiver operating characteristic curve analysis. A nomogram and risk model of sorafenib-response target genes enabled the evaluation of the prognosis of patients with HCC. The relationship between risk scores and levels of infiltrating immune cells was visualized via correlation analysis. We identified 1620 sorafenib-response target genes involved in the PPAR signaling pathway, antigen processing and presentation, and ferroptosis. SLC41A3, SEC61A1, LRP4, PPM1G, and HSP90AA1 were independent risk factors for a poor prognosis for patients with HCC and had diagnostic value. A risk model based on SLC41A3, SEC61A1, LRP4, PPM1G, and HSP90AA1 expression showed that patients with HCC in the high-risk group had a worse prognosis. Consensus-clustering analysis (performed with K set to 2) distinguished two clusters (the cluster 1 and cluster 2 groups). Patients in cluster 1 survived significantly longer than those in cluster 2. The risk score correlated with the levels of T cells, cytotoxic lymphocytes, CD8 T cells, macrophages, memory B cells, follicular helper T cells, and other immune cells. The high risk based on the sorafenib-response targets SLC41A3, SEC61A1, LRP4, PPM1G, and HSP90AA1 represented the poor prognosis for patients with HCC and significantly correlated with the levels of immune infiltrating cells in HCC.
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http://dx.doi.org/10.1155/2022/7257738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256406PMC
June 2022

Loosely-packed dynamical structures with partially-melted surface being the key for thermophilic argonaute proteins achieving high DNA-cleavage activity.

Nucleic Acids Res 2022 07;50(13):7529-7544

School of Physics and Astronomy, Institute of Natural Sciences, School of Medicine, Shanghai National Center for Applied Mathematics (SJTU center), Zhangjiang Institute for Advanced Study, Shanghai Jiao Tong University, Shanghai 200240, China.

Prokaryotic Argonaute proteins (pAgos) widely participate in hosts to defend against the invasion of nucleic acids. Compared with the CRISPR-Cas system, which requires a specific motif on the target and can only use RNA as guide, pAgos exhibit precise endonuclease activity on any arbitrary target sequence and can use both RNA and DNA as guide, thus rendering great potential for genome editing applications. Hitherto, most in-depth studies on the structure-function relationship of pAgos were conducted on thermophilic ones, functioning at ∼60 to 100°C, whose structures were, however, determined experimentally at much lower temperatures (20-33°C). It remains unclear whether these low-temperature structures can represent the true conformations of the thermophilic pAgos under their physiological conditions. The present work studied three pAgos, PfAgo, TtAgo and CbAgo, whose physiological temperatures differ significantly (95, 75 and 37°C). By conducting thorough experimental and simulation studies, we found that thermophilic pAgos (PfAgo and TtAgo) adopt a loosely-packed structure with a partially-melted surface at the physiological temperatures, largely different from the compact crystalline structures determined at moderate temperatures. In contrast, the mesophilic pAgo (CbAgo) assumes a compact crystalline structure at its optimal function temperature. Such a partially-disrupted structure endows thermophilic pAgos with great flexibility both globally and locally at the catalytic sites, which is crucial for them to achieve high DNA-cleavage activity. To further prove this, we incubated thermophilic pAgos with urea to purposely disrupt their structures, and the resulting cleavage activity was significantly enhanced below the physiological temperature, even at human body temperature. Further testing of many thermophilic Agos present in various thermophilic prokaryotes demonstrated that their structures are generally disrupted under physiological conditions. Therefore, our findings suggest that the highly dynamical structure with a partially-melted surface, distinct from the low-temperature crystalline structure, could be a general strategy assumed by thermophilic pAgos to achieve the high DNA-cleavage activity.
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http://dx.doi.org/10.1093/nar/gkac565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303296PMC
July 2022

The Significant Role of Tumor Volume on the Surgical Approach Choice, Surgical Complexity, and Postoperative Complications in Renal Cell Carcinoma With Venous Tumor Thrombus From a Large Chinese Center Experience.

Front Oncol 2022 7;12:869891. Epub 2022 Jun 7.

Department of Urology, Peking University Third Hospital, Beijing, China.

Objective: To explore the role of tumor volume (TV) on surgical approach choice, surgical complexity, and postoperative complications in patients with renal cell carcinoma (RCC) and inferior vena cava tumor thrombus.

Method: From January 2014 to January 2020, we retrospectively analyzed the clinical data of 132 patients who underwent radical nephrectomy with inferior vena cava thrombectomy (RN-IVCT). Primary renal tumor volume (PRTV), renal vein tumor thrombus volume (RVTTV), inferior vena cava tumor thrombus volume (IVCTTV), and total tumor thrombus volume (TTTV) were measured with the help of an internationally recognized 3D volume measurement software. The patients were divided into three groups according to the tumor volume within the inferior vena cava (IVC). Group 1 included 48 patients with IVCTTV between 0 and 15 cm3 (36.6%), group 2 included 38 patients with IVCTTV between 16 and 30 cm3 (28%), and group 3 included 46 patients with IVCTTV above 30 cm3 (35%). The three IVCTTV groups, as well as four different volume groups, were compared in terms of surgical approach choice, surgical complexity, and postoperative complications. One-way ANOVA and a non-parametric test were used to compare the clinicopathological characteristics and distribution differences between the three groups.

Result: This study found significant differences among the three groups in the proportion of open surgery (P < 0.001), operation time (P < 0.044), intraoperative bleeding (P < 0.001), and postoperative complications (P < 0.001). When the four different volumes were compared, we found that for higher volumes IVCTTV and TTTV, open surgery is used more often compared with laparoscopic surgery (P < 0.001). In addition, with the increase in renal vein tumor thrombus volume, inferior vena cava tumor thrombus volume, and total tumor thrombus volume, the operation time also increased. Finally, with the increase in tumor thrombus volume and total tumor thrombus volume, the amount of intraoperative bleeding increased.

Conclusion: With the increase in tumor volume, the proportion of open surgery and the incidence of postoperative complications increased. In addition, larger tumor volume prolongs operation time, increases intraoperative blood loss, and makes the surgery more complicated.
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http://dx.doi.org/10.3389/fonc.2022.869891DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209712PMC
June 2022

Sea spray aerosols intervening phospholipids ozonolysis at the air-water interface.

J Hazard Mater 2022 05 10;430:128466. Epub 2022 Feb 10.

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, Heilongjiang 150090, PR China; School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150090, PR China. Electronic address:

With more than half of the world's population lives along the coast and in its vicinity, the sea spray aerosols (SSAs) with respect to respiratory system impact has attracted increasing attention. In this paper, ozonolysis of model lung phospholipids intervened by salt cations in SSAs at air-water interface was investigated using acoustic levitation-nano-electrospray ionization-mass spectrometry (AL-nano-ESI-MS). The cation species facilitated the interfacial ozonolysis of phospholipids, and this increased ozonolysis showed a dependence on the concentration of salt cations. The charge number and ion radius of salt cations were also investigated, and the times of increased efficiency for phospholipids ozonolysis at the air-water interface were higher with more charge numbers or lower ion radius. The mechanism study revealed that the electrostatic interaction between the electronegative headgroup of phospholipids and the cations disturbed the packing of phospholipids, and resulted in oleyl chains more vulnerable with ozone. Finally, aerosolization of the salt-dominated artificial seawater and real seawater revealed a significant increase on ozonolysis of phospholipid intervened by salt cations. These results reveal SSAs intervening phospholipids interfacial reaction at the molecule level, which will be beneficial to gain the knowledge of the negative health effect concerning the components involved in SSAs.
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http://dx.doi.org/10.1016/j.jhazmat.2022.128466DOI Listing
May 2022

Engineered coxsackievirus B3 containing multiple organ-specific miRNA targets showed attenuated viral tropism and protective immunity.

Infect Genet Evol 2022 Jun 17;103:105316. Epub 2022 Jun 17.

Department of Biochemistry and Immunology, Capital Institute of Pediatrics, Beijing, China; Beijing Municipal Key Laboratory of Child Development and Nutriomics, Beijing, China; Beijing Tongren Hospital, Capital Medical University, Beijing, China. Electronic address:

Coxsackievirus B3 (CVB3) can cause viral myocarditis, pancreatitis, and aseptic meningitis. This study aimed to construct an engineered CVB3 harboring three different tissue-specific miRNA targets (CVB3-miR3*T) to decrease the virulence of CVB3 in muscles, pancreas, and brain. CVB3-miR3*T and CVB3-miR-CON (containing three sequences not found in the human genome) were engineered and replicated in HELA cells. A viral plaque assay was used to determine the titers in HELA cells and TE671 cells (high miRNA-206 expression), MIN-6 cells (high miRNA-29a-3p expression), and mouse astrocytes (high miRNA-124-3p expression). We found that engineered CVB3 showed attenuated replication and reduced cytotoxicity, the variability of each type of cell was also increased in the CVB3-miR3*T group. Male BALB/c mice were infected to determine the LD50 and examine heart, pancreas, and brain titers and injury. Viral replication of the engineered viruses was restricted in infected mouse heart, pancreas, and brain, and viral plaques were about 100 fold lower compared with the control group. Mice immunized using CVB3-miR3*T, UV-inactivated CVB3-WT, and CVB3-miR-CON were infected with 100 × LD50 of CVB3-WT to determine neutralization. CVB3-miRT*3-preimmunized mice exhibited complete protection and remained alive after lethal virus infection, while only 5/15 were alive in the UV-inactivated mice, and all 15 mice were dead in the PBS-immunized group. The results demonstrate that miR-206-, miRNA-29a-3p-, and miRNA-124-3p-mediated CVB3 detargeting from the pancreas, heart, and brain might be a highly effective strategy for viral vaccine development.
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http://dx.doi.org/10.1016/j.meegid.2022.105316DOI Listing
June 2022

Breast Cancer Prognosis Prediction and Immune Pathway Molecular Analysis Based on Mitochondria-Related Genes.

Genet Res (Camb) 2022 31;2022:2249909. Epub 2022 May 31.

Institute of Innovation and Applied Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China.

Background: Mitochondria play an important role in breast cancer (BRCA). We aimed to build a prognostic model based on mitochondria-related genes.

Method: Univariate Cox regression analysis, random forest, and the LASSO method were performed in sequence on pretreated TCGA BRCA datasets to screen out genes from a Gene Set Enrichment Analysis, Gene Ontology: biological process gene set to build a prognosis risk score model. Survival analyses and ROC curves were performed to verify the model by using the GSE103091 dataset. The BRCA datasets were equally divided into high- and low-risk score groups. Comparisons between clinical features and immune infiltration related to different risk scores and gene mutation analysis and drug sensitivity prediction were performed for different groups.

Result: Four genes, MRPL36, FEZ1, BMF, and AFG1L, were screened to construct our risk score model in which the higher the risk score, the poorer the prognosis. Univariate and multivariate analyses showed that the risk score was significantly associated with age, M stage, and N stage. The gene mutation probability in the high-risk score group was significantly higher than that in the low-risk score group. Patients with higher risk scores were more likely to die. Drug sensitivity prediction in different groups indicated that PF-562271 and AS601245 might be new inhibitors of BRCA.

Conclusion: We developed a new workable risk score model based on mitochondria-related genes for BRCA prognosis and identified new targets and drugs for BRCA research.
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http://dx.doi.org/10.1155/2022/2249909DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174003PMC
May 2022

Design, synthesis and anti-breast cancer activity of novel fluorinated 7-O-modified genistein derivatives.

Med Chem 2022 Jun 7. Epub 2022 Jun 7.

Department of Pharmacy, Hengyang Medicinal School, University of South China, Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug research, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, the Second Affiliated Hospital of University of South China, Hengyang, Hunan, 421001, China.

Background: Genistein has been limited in clinical application due to its low bioavailability, extremely poor liposolubility, and fast glycosylation rate, though it possesses anti-breast cancer activity. Therefore, the discovery of novel genistein derivatives is an urgency.

Objective: To enhance the anti-breast cancer activity of genistein, a series of novel fluorinated genistein derivatives were synthesized.

Method: Their in vitro antitumor activity was investigated by the MTT assay against three cancer cell lines, via., MDA-MB-231, MCF-7 and MDA-MB-435, respectively.

Results: Analogs 1d, 2b, 3b showed remarkable anticancer activities comparing to tamoxifen, a clinical anti-breast cancer drug on the market.

Conclusion: The activities against breast cancer of genistein were enhanced by introducing 7-alkoxyl group and fluorine atom into the B-ring. Therefore, these compounds may be potential candidates for treating breast cancer.
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http://dx.doi.org/10.2174/1573406418666220607140651DOI Listing
June 2022

The Brassicaceae Genome Resource (TBGR): a comprehensive genome platform for Brassicaceae plants.

Plant Physiol 2022 Jun 7. Epub 2022 Jun 7.

School of Life Sciences, North China University of Science and Technology, Tangshan, Hebei 063210, China.

The Brassicaceae is an important plant family. We built a user-friendly, web-based, comparative, and functional genomic database, The Brassicaceae Genome Resource (TBGR, http://www.tbgr.org.cn), based on 82 released genomes from 27 Brassicaceae species. The TBGR database contains a large number of important functional genes, including 4,096 glucosinolate genes, 6,625 auxin genes, 13,805 flowering genes, 36,632 resistance genes, 1,939 anthocyanin genes, and 1,231 m6A genes. A total of 1,174,049 specific guide sequences for CRISPR and 5,856,479 transposable elements were detected in Brassicaceae. TBGR also provides information on synteny, duplication, and orthologs for 27 Brassicaceae species. The TBGR database contains 1,183,851 gene annotations obtained using the TrEMBL, Swiss-Prot, Nr, GO, and Pfam databases. The BLAST, Synteny, Primer Design, Seq_fetch, and JBrowse tools are provided to help users perform comparative genomic analyses. All the genome assemblies, gene models, annotations, and bioinformatics results can be easily downloaded from the TBGR database. We plan to improve and continuously update the database with newly assembled genomes and comparative genomic studies. We expect the TBGR database to become a key resource for the study of the Brassicaceae.
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http://dx.doi.org/10.1093/plphys/kiac266DOI Listing
June 2022

Robot-Assisted Laparoscopic IVC Treatment Strategy in Retroperitoneal Tumors.

Front Oncol 2022 20;12:908272. Epub 2022 May 20.

Department of Urology, Peking University Third Hospital, Haidian District, Beijing, China.

Objectives: To show the practice of robot-assisted laparoscopic inferior vena cava (IVC) treatment strategies in patients with retroperitoneal tumors.

Patients And Methods: From October 2020 to July 2021, 17 patients with retroperitoneal tumors successfully underwent robot-assisted laparoscopic tumor resection with IVC management. The patient details, tumor characteristics, intraoperative data, pathological features and severe complications were assessed. The IVC treatment strategies were divided into 4 ways: ①local resection and primary repair of the IVC; ②IVC ligation; ③ IVC reconstruction by bovine pericardial grafts; and ④ IVC transection and anastomosis.

Results: In terms of IVC management, 5 cases had conventional total occlusion of the IVC and its branches, 3 cases had delayed occlusion of the proximal IVC technique, 2 cases had IVC resection by Satinsky clamp, 5 cases had IVC ligation, 1 case had IVC reconstruction by bovine pericardial grafts and 1 case had IVC transection and anastomosis. The median operation time was 151 min, and blood loss was 500 ml. There was no severe complication perioperatively. The follow-up time of 17 patients was 8 to 17 months (median: 12 months). No local recurrence or overall death was found during follow-up.

Conclusions: These robot-assisted laparoscopic IVC treatment strategies were considered to be safe and feasible in experienced centers, as well as helpful to completely remove the tumor for better oncological prognosis and restore the blood reflux of IVC as much as possible to ensure fewer postoperative complications.
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http://dx.doi.org/10.3389/fonc.2022.908272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166303PMC
May 2022

Safety Evaluation of Chemical Insecticides to (Hymenoptera: Eulophidae), a Pupal Parasitoid of (Lepidoptera: Noctuidae) Using Three Exposure Routes.

Insects 2022 May 7;13(5). Epub 2022 May 7.

Key Laboratory of Integrated Pest Management on Tropical Crops of Ministry of Agriculture and Rural Affairs, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.

has become a major pest in many crops worldwide. The main control strategies are biological and chemical controls. However, pesticides have varying degrees of toxicity to parasitic wasps in the field. To integrate chemical and biological controls, we evaluated the safety of insecticides to , an important pupal parasitoid of . This study assessed the toxicity of six major control insecticides (emamectin benzoate, chlorfenapyr, indoxacarb, chlorantraniliprole, bisultap, and lufenuron) to based on risk quotient. The results showed that indoxacarb had the lowest risk quotient (RQ = 7.43). Then the side effects of three sublethal concentrations (LC, LC, LC) of indoxacarb were tested using three methods (1. Adult exposure to pesticide residues on the glass tube; 2. Adult exposure to pesticide residues on the host; 3. Larval exposure to pesticides through host exposure). Overall, . had a lower parasitism rate and emergence rate with the higher pesticide concentrations. Furthermore, among three methods, the adult exposure to pesticide residues on the glass tube was the most efficient in inhibiting the parasitism rate, and impairing the emergence rate and the offspring female/male ratio. This study guides a more scientific and comprehensive application of pesticides and releases natural enemies in the field.
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http://dx.doi.org/10.3390/insects13050443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143371PMC
May 2022

Clinical Experience and Management Strategy of Retroperitoneal Tumor With Venous Tumor Thrombus Involvement.

Front Oncol 2022 10;12:873729. Epub 2022 May 10.

Department of Urology, Peking University Third Hospital, Beijing, China.

Background: This study aims to report the surgical management, complications, and outcomes for patients with retroperitoneal tumor and venous thrombus.

Methods: We retrospectively analyzed 19 cases of retroperitoneal tumor with venous tumor thrombus from August 2015 to March 2021. A new tumor thrombus PUTH-RT grading system was proposed on the basis of the characteristics of the surgical techniques.

Results: Two cases of PUTH-RT-1a, two cases of PUTH-RT-1b, six cases of PUTH-RT-2, six cases of PUTH-RT-3, and three cases of PUTH-RT-4 were included. Surgeries were successfully performed in all 19 patients. Among them, five cases (26.3%) were operated a completely laparoscopic approach and 13 cases (68.4%) an open approach. One case (5.3%) was converted from laparoscopic to open approach. Five cases (26.3%) experienced postoperative complications. All patients were followed for a median of 14 months. Cancer-associated death occurred in three cases. Distant metastases occurred in seven cases.

Conclusions: We propose a new tumor thrombus grading system based on the anatomical characteristics of retroperitoneal tumors with venous tumor thrombus. Retroperitoneal tumor resection and removal of venous tumor thrombi are safe and effective for the treatment of such diseases.
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http://dx.doi.org/10.3389/fonc.2022.873729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9128060PMC
May 2022

Tiny Ni Nanoparticles Embedded in Boron- and Nitrogen-Codoped Porous Carbon Nanowires for High-Efficiency Water Splitting.

ACS Appl Mater Interfaces 2022 Jun 23;14(21):24447-24461. Epub 2022 May 23.

National and Local Joint Engineering Laboratory for Lithium-Ion Batteries and Materials Preparation Technology, Key Laboratory of Advanced Battery Materials of Yunnan Province, Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, PR China.

The integration of nickel (Ni) nanoparticle (NP)-embedded carbon layers ([email protected]) into the three-dimensional (3D) hierarchically porous carbon architectures, where ultrahigh boron (B) and nitrogen (N) doping is a potential methodology for boosting Ni catalysts' water splitting performances, was achieved. In this study, the novel 3D ultrafine Ni NP-embedded and B- and N-codoped hierarchically porous carbon nanowires (denoted as [email protected]) were successfully synthesized via pyrolysis of the corresponding 3D nickel acetate [Ni(AC)·4HO]-hydroxybenzeneboronic acid-polyvinylpyrrolidone precursor networks woven by electrospinning. After optimizing the pyrolysis temperatures, various structural and morphological characterization analyses indicate that the optimal [email protected] networks own a large surface area, abundant micro/mesopores, and vast carbon edges/defects, which boost doping a large amount of B (5.81 atom %) and N (5.84 atom %) dopants into carbon frameworks with 6.36 atom % of BC, pyridinic-N (pyridinic-N-Ni), and graphitic-N active sites. Electrochemical measurements demonstrate that [email protected] reveals the best hydrogen evolution reaction (HER) and oxygen reduction reaction catalytic activities in an alkaline solution. The HER potential at 10 mA cm [ = -164.2 mV reversible hydrogen electrode (RHE)] of the optimal [email protected] is just 96.2 mV more negative than that of the state-of-the-art 20 wt % Pt/C ( = -68 mV RHE). In particular, the OER and Tafel slope of the optimal [email protected] (1.517 V RHE and 19.31 mV dec) are much smaller than those of RuO (1.557 V RHE and 64.03 mV dec). For full water splitting, the catalytic current density achieves 10 mA cm at a low cell voltage of 1.584 V for the (-) [email protected]||[email protected] (+) electrolysis cell, which is 10 mV smaller than that of the (-) 20 wt % Pt/C||RuO (+) benchmark (1.594 V) under the same conditions. The synergistic effects of 3D hierarchically porous structures, advanced charge transport ability, and abundant active centers [such as [email protected], BC, pyridinic-N (pyridinic-N-Ni), and graphitic-N] are responsible for the excellent water-splitting catalytic activity of the [email protected] networks. Especially, because of the remarkable structural and chemical stabilities of 3D hierarchically porous [email protected] networks, the (-) [email protected]||[email protected] (+) water electrolysis cell displays an excellent stability.
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http://dx.doi.org/10.1021/acsami.2c04956DOI Listing
June 2022
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