Publications by authors named "Zhongliang Su"

7 Publications

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The analgesic evaluation of gabapentin for arthroscopy: A meta-analysis of randomized controlled trials.

Medicine (Baltimore) 2021 May;100(20):e25740

Department of Orthopedics, Affiliated Yueqing Hospital,Wenzhou Medical University, Wenzhou, Zhejiang Province, P.R. China.

Introduction: The efficacy of gabapentin for pain management of arthroscopy remains controversial. We conduct a systematic review and meta-analysis to explore the influence of gabapentin versus placebo on the postoperative pain intensity of arthroscopy.

Methods: We search PubMed, EMbase, Web of science, EBSCO, and Cochrane library databases through April 2020 for randomized controlled trials assessing the effect of gabapentin versus placebo on pain control of arthroscopy. This meta-analysis is performed using the random-effect model.

Results: Five randomized controlled trials are included in the meta-analysis. Overall, compared with control group for arthroscopy, gabapentin remarkably decreases pain scores at 24 hour (standard mean difference [SMD]=-0.68; 95% confidence interval [CI]=-1.15 to -0.02; P = .21), analgesic consumption (SMD = -18.24; 95% CI=-24.61 to -11.88; P < .00001), nausea and vomiting (OR = 0.42; 95% CI = 0.21 to 0.84; P = .01), but has no obvious influence on pain scores at 6 h (SMD = -1.30; 95% CI = -2.92 to 0.31; P = .11) or dizziness (OR = 1.12; 95% CI = 0.56 to 2.24; P = .75).

Conclusions: Gabapentin is effective for pain control after arthroscopy.
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http://dx.doi.org/10.1097/MD.0000000000025740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137103PMC
May 2021

Predicting copper contamination in wheat canopy during the full growth period using hyperspectral data.

Environ Sci Pollut Res Int 2020 Nov 8;27(31):39029-39040. Epub 2020 Jul 8.

College of Chemical engineering, Qingdao University of Science & Technology, Qingdao, 266042, China.

The rapid and efficient determination of heavy metal content in food crops is essential for human health and environmental protection. The use of hyperspectral data has become a popular way to predict heavy metal content in plants; however, many challenges remain. One challenge is that lab conditions differ from actual agricultural production conditions. Another challenge is that spectral data characteristics are not universally applicable to all situations. Therefore, in this study, the field test method was adopted to conduct experiments during the full growth period of wheat, and the spectrum data of wheat canopy were processed by the first derivative method to screen-sensitive spectral bands as the basis for the prediction model of the copper content in wheat. The results showed that the copper content increased with an increase in the soil copper content, and there were dissimilar subtle differences in the spectral reflectance of wheat canopy under different stressed soil copper concentrations; sensitive spectral indices and wavelengths were screened based on good correlation with the copper content in the wheat canopy. Different optimal predicting models in different periods were built and verified. The established linear regression models, which were based on NDVI/SIPI and W728, were the most suitable predicting models during the tillering stage with R = 0.669 and 0.818; Rg, W741, and multiple bands were the most suitable predicting models during the jointing stage with R = 0.548, 0.830, and 0.868; the optimal model during the heading stage was based on W480 (R = 0.625). This study demonstrated that the constructed models had good potential for estimating the copper content in wheat leaves during full growth periods, and this method had the potential to be applied to the actual agricultural production process.
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http://dx.doi.org/10.1007/s11356-020-09973-wDOI Listing
November 2020

Identification of Novel Compound Heterozygous Mutations in the Gene of a Chinese Patient Diagnosed With Giant Axonal Neuropathy.

Front Neurosci 2020 25;14:85. Epub 2020 Feb 25.

Wenzhou People's Hospital, Wenzhou Maternal and Child Health Care Hospital, The Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou, China.

Giant axonal neuropathy (GAN) is a very rare autosomal recessive disorder characterized by abnormally large and dysfunctional neuronal axons. Mutations in the gene have been identified as the cause of this disorder. In this report, we performed a detailed phenotypic assessment of a Chinese patient with GAN. An array-based exon capture test and targeted next-generation sequencing were used to detect the suspected mutation sites. Compound heterozygous mutations of p.S79L (c.236C > T) in the BTB domain and p.T489S (c.1466C > G) in the kelch domain were identified in the proband's genome. S79L was a known mutation, and T489S was reported for the first time. The p.S79L and p.T489S were confirmed in the proband's mother and father, respectively. Both mutations were located in highly conserved regions and affected the predicted protein crystal structures. The proband's sural biopsy revealed the classical GAN phenotype of swollen axons filled with closely packed neurofilaments. The combined application of the next-generation sequencing platform and bioinformatics analyses was an effective method for diagnosing GAN. The novel compound mutations of S79L and T489S in the gene were likely the cause of the patient's GAN symptoms. Our findings enrich the spectrum of mutations associated with this rare type of axonopathy.
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http://dx.doi.org/10.3389/fnins.2020.00085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052293PMC
February 2020

Crystal structure of enolase from Drosophila melanogaster.

Acta Crystallogr F Struct Biol Commun 2017 04 22;73(Pt 4):228-234. Epub 2017 Mar 22.

College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, Shandong 266042, People's Republic of China.

Enolase is an important enzyme in glycolysis and various biological processes. Its dysfunction is closely associated with diseases. Here, the enolase from Drosophila melanogaster (DmENO) was purified and crystallized. A crystal of DmENO diffracted to 2.0 Å resolution and belonged to space group R32. The structure was solved by molecular replacement. Like most enolases, DmENO forms a homodimer with conserved residues in the dimer interface. DmENO possesses an open conformation in this structure and contains conserved elements for catalytic activity. This work provides a structural basis for further functional and evolutionary studies of enolase.
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http://dx.doi.org/10.1107/S2053230X17004022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379173PMC
April 2017

Properties of chitosan-immobilized cellulase in ionic liquid.

Biotechnol Appl Biochem 2013 Mar-Apr;60(2):231-5. Epub 2013 Jan 11.

Department of Biology, Qingdao University of Science and Technology, Qingdao, People's Republic of China.

The properties of cellulase that was attached to the surface of the chitosan carrier in aqueous-ionic liquid (IL; 1,3-dimeth-ylimidazolium dimethylphosphate) mixture were studied. The optimal temperature for immobilized cellulase in aqueous-IL mixed solutions was 60 °C. The immobilized cellulase acquired the highest relative activity at a ratio of 1:4 (IL to water, v/v), compared to activity levels of 79% and 7%, when the ratio of IL to water (v/v) was 0:1 and 1:0, respectively. At 80 °C, the immobilized cellulase in the aqueous-IL mixture conserved 46.3% activity after 120 Min. The immobilized cellulase can be effectively reused three times. After 4 weeks, the activity of immobilized cellulase maintained 83.5%. The Michaelis constant (Km ) and maximum reaction velocity (Vm ) values for the immobilized cellulase were 4.8 mg/L and 0.156 mg/(mL Min), respectively. To the best of our knowledge, this is the first report on the properties of chitosan-immobilized cellulase in aqueous-IL.
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http://dx.doi.org/10.1002/bab.1057DOI Listing
November 2013

Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-phycocyanin in Escherichia coli by using one expression vector.

Appl Biochem Biotechnol 2007 Jul;142(1):52-9

College of Marine Life Science, Faculty of Life Science and Technology, Ocean University of China, Qingdao, People's Republic of China.

The phycobiliproteins (PBSs) are large pigment proteins found in certain algae that play a central role in harvesting light energy for photosynthesis. Phycocyanin (PC) is one type of PBSs that gains increasing attention owing to its various biological and pharmacological properties. In this paper, an expression vector containing five essential genes in charge of biosynthesis of cyanobacterial C-phycocyanin (C-PC) holo-alpha subunit (holo-CpcA) was successfully constructed resulting in over-expression of a fluorescent holo-CpcA in E. coli BL21. The vector harbored two cassettes: one cassette carried genes hox1 and pcyA required for conversion of heme to phycocyanobilin (PCB), and the other cassette carried cpcA encoding CpcA along with cpcE and cpcF both of which were necessary and sufficient for the correct addition of PCB to CpcA. The vector system contained a His-tag for protein purification. The purified protein showed correct molecular weight on SDS-PAGE gel and emitted orange fluorescence by UV excitation. The maximum peak of absorbance spectrum was at 623 nm, and the maximum peak of fluorescence emission and excitation were at 648 and 633 nm, respectively, which were similar to those of native C-PC. This study provides an efficient method for large-scale production of the fluorescent holo-CpcA in biotechnological applications.
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http://dx.doi.org/10.1007/s12010-007-8000-7DOI Listing
July 2007

A simple preparation method of crystals of soybean hull peroxidase.

Appl Microbiol Biotechnol 2007 Feb 6;74(1):249-55. Epub 2006 Oct 6.

Qingdao University of Science and Technology, P.O. Box 70, Qingdao 266042, People's Republic of China.

Soybean hull peroxidase (SHP) was crystallised from an enzyme solution with low purity by a simple method. The enzyme solution was purified by cooperation salting out of acetone and ammonium sulphate, and lumpy crystals were obtained with the size of about 40 x 30 mum when ammonium sulphate was quickly added to the enzyme solution. The crystal was examined and confirmed to be an SHP crystal by the method of activity test. The result shows that, though the purity of the enzyme solution was not high, crystals could be formed when the enzyme solution rapidly reached to a degree of supersaturation, which was different from the traditional methods of protein crystallisation. Additionally, a purification method of acetone and ammonium sulphate fractional salting out was also studied, in which the procedure was simplified, and a satisfactory purification effect was obtained.
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http://dx.doi.org/10.1007/s00253-006-0639-4DOI Listing
February 2007
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