Publications by authors named "Zhong Liu"

458 Publications

[Identification of intestine direct targets of Shouhui Tongbian Capsules using "target fishing" strategy].

Zhongguo Zhong Yao Za Zhi 2021 Feb;46(3):505-510

State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University Beijing 100191,China.

"Target fishing" strategy was used to investigate the direct targets and mechanism of Shouhui Tongbian Capsules on relaxing bowel. Magnetic beads cross-linked with the chemical constituents from Shouhui Tongbian Capsules were prepared. The potential target proteins were captured from the total protein lysates of rat intestine using the beads. The captured proteins were further identified by LC-MS/MS, and the associated pathways were analyzed by Cytoscape. RESULTS:: showed that 138 potential target proteins were identified, which were involved in eight signaling pathways, including tricarboxylic acid cycle, pyrimidine metabolism, sulfur metabolism, fatty acid degradation, alanine/aspartate/glutamate metabolism, arginine/proline metabolism, valine/leucine/isoleucine degradation, and β-alanine metabolism. Taken together, Shouhui Tongbian Capsules may exert relaxing bowel effect by acting on multiple signaling pathways to promote intestinal gurgling, inhibit inflammation, as well as improve intestinal barrier function, intestinal water secretion, and intestinal flora.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20201125.403DOI Listing
February 2021

Donor tolerability of convalescent plasma donation.

J Clin Apher 2021 Feb 15. Epub 2021 Feb 15.

Clinical Transfusion Research Center, Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, Sichuan Province, P.R. China.

Background: Since early 2020, convalescent plasma has been widely used for treating coronavirus disease 2019 (COVID-19). There is limited information regarding donor tolerability of convalescent plasma donation. In this study, we evaluated the short-term donor tolerability of convalescent plasma donation.

Methods: A prospective study of 309 convalescent plasma donation related adverse events were conducted at Wuhan Blood Center of China, from February 12 to April 1, 2020. Additionally, up to 28-day post-donation follow-ups were performed on the donors.

Results: Sixteen (5.2%) adverse events were reported in 309 donations. All of these were mild vasovagal without loss of consciousness. The frequency of adverse reactions was higher in donors with a per donation volume of >8 mL/kg body weight or ≥ 600 mL, <100 mm Hg in pre-donation systolic blood pressure, or less than 28 days from the onset of COVID-19 symptoms. There was no correlation to donation history, weight, sex, ABO blood type, pre-donation diastolic blood pressure, pulse, or hemoglobin.

Conclusion: The donation of convalescent plasma is generally safe. Mitigation of risk factors associated with adverse events can further enhance donor tolerability of convalescent plasma donation.
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http://dx.doi.org/10.1002/jca.21882DOI Listing
February 2021

Lab-scale design of two layers wood cellulose filter media to maximize life span for intake air filtration.

Sci Rep 2021 Feb 4;11(1):3153. Epub 2021 Feb 4.

Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science and Technology, Tianjin, 300457, China.

The requirement of continuous and stringent growth on filtration performance, including longer life span, higher overall efficiency, lower initial pressure drop and more cost effective, has still drove filter media manufactures to research and develop. One of the possible way to achieve these challenges, was to utilize a dual-channel head-box with two sets of pulp conveying system, which can produce filter media with bulky and gradient properties. In this study, three kinds of commercial cellulose were chosen to make two layers filtration media, analyzed the effect of fiber blend on physical properties and filtration performance. By fine-tune the slurry ratio of top layer, we made one single layer and two layers composition filter media, the thickness and air permeability of composition media were higher than single layer media. According to ISO 5011, filtration performance test has been done to compare single layer media with composition media, this composition gradient profiles that provided the life span 37.0% improvement to the terminal pressure drop during dust injecting, and the dust hold capacity improved 34.7%, the main contributor of dust hold capacity was decided by top layer, however, the overall efficiency was depended on wire side layer.
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http://dx.doi.org/10.1038/s41598-021-82855-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862255PMC
February 2021

Epidemiological survey and screening strategy for dengue virus in blood donors from Yunnan Province.

BMC Infect Dis 2021 Jan 22;21(1):104. Epub 2021 Jan 22.

Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, USA.

Background: Dengue virus (DENV) infection is increasingly common in southern China and can be transmitted through blood transfusion but is not currently part of donor screening throughout the region. We assessed DENV prevalence among donors at the Xishuangbanna Blood Center, Yunnan, to support development of DENV screening strategies.

Methods: Blood samples were collected randomly between June 2019 and August 2019. These were screened for anti-DENV IgG and IgM using enzyme-linked immunosorbent assay (ELISA). Then, all reactive samples and some randomly-chosen non-reactive samples were used to detect DENV RNAs using real-time polymerase-chain-reaction (RT-PCR) assays. After RT-PCR, samples were further tested for soluble nonstructural protein 1 (NS1) using the colloidal gold method. Donors demographics were also collected and assessed.

Results: Over the study period, 2254 donor samples were collected and tested for anti-DENV IgG and IgM by ELISA. This revealed 598 anti-DENV IgG and/or IgM reactive samples, a serological prevalence of 26.53%. Of these, 26 were RT-PCR positive and/or NS1 positive. Significant differences in DENV prevalence were noted by occupation (P = 0.001), education (P < 0.001), and ethnicity (P = 0.026).

Conclusion: The prevalence of DENV in Xishuangbanna Blood Center was higher than most other blood centers that have implemented DENV donor screening. Our study provides first-hand data about the prevalence of DENV and allows the development of a screening strategy for clinical use.
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http://dx.doi.org/10.1186/s12879-021-05810-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821518PMC
January 2021

First annual report of Chinese haemovigilance network.

Vox Sang 2021 Jan 10. Epub 2021 Jan 10.

Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu, China.

Background And Objectives: Haemovigilance involves surveillance of the whole chain of blood transfusion with the aim of identifying adverse events and errors and improving outcomes for patients. The Chinese Haemovigilance Network, founded in August 2017, has witnessed a rapid development in the last three years.

Materials And Methods: Based on the 1,022 cases in 2019, we analysed the adverse reactions (ARs) by blood component, clinical outcome severity and demography of recipients in an effort to publish the first annual Chinese haemovigilance report.

Results: The AR rate associated with blood transfusion in 2019 was 0·2% in China. Allergic reactions and FNHTR were the two most common adverse symptoms, accounting for 97·7% of the reports. Two-thirds of the TAD, AHTR and TACO and all of the HTR and DHTR resulted in hospitalization or prolongation of hospitalization. Plasma and AP were usually associated with allergic reaction (81·1%), whereas red cells more commonly cause FNHTR (68·8%) and all the AHTR, HTR, DSTR and DHTR. 84·1% of patients were aged 16 years or over, and the majority of the TAD, AHTR, TACO and HTR involved patients aged 60 and above. The ratio of serious adverse reactions (SARs) was 8·2%. Allergic reaction and FNHTR were top two (85·7%) SARs. The first case related to anti-D immunoglobulin was detected in a DHTR report.

Conclusion: This report provides the world's first overview of transfusion-related adverse reactions in China. This report is useful for better understanding transfusion risks in China.
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http://dx.doi.org/10.1111/vox.13059DOI Listing
January 2021

Analysis of viral load in different specimen types and serum antibody levels of COVID-19 patients.

J Transl Med 2021 01 7;19(1):30. Epub 2021 Jan 7.

Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 610052, Sichuan, People's Republic of China.

Background: COVID-19 has caused a global pandemic and the death toll is increasing. However, there is no definitive information regarding the type of clinical specimens that is the best for SARS-CoV-2 detection, the antibody levels in patients with different duration of disease, and the relationship between antibody level and viral load.

Methods: Nasopharyngeal swabs, anal swabs, saliva, blood, and urine specimens were collected from patients with a course of disease ranging from 7 to 69 days. Viral load in different specimen types was measured using droplet digital PCR (ddPCR). Meanwhile, anti-nucleocapsid protein (anti-N) IgM and IgG antibodies and anti-spike protein receptor-binding domain (anti-S-RBD) IgG antibody in all serum samples were tested using ELISA.

Results: The positive detection rate in nasopharyngeal swab was the highest (54.05%), followed by anal swab (24.32%), and the positive detection rate in saliva, blood, and urine was 16.22%, 10.81%, and 5.41%, respectively. However, some patients with negative nasopharyngeal swabs had other specimens tested positive. There was no significant correlation between antibody level and days after symptoms onset or viral load.

Conclusions: Other specimens could be positive in patients with negative nasopharyngeal swabs, suggesting that for patients in the recovery period, specimens other than nasopharyngeal swabs should also be tested to avoid false negative results, and anal swabs are recommended. The antibody level had no correlation with days after symptoms onset or the viral load of nasopharyngeal swabs, suggesting that the antibody level may also be affected by other factors.
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http://dx.doi.org/10.1186/s12967-020-02693-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790347PMC
January 2021

Thyroid nodule recognition using a joint convolutional neural network with information fusion of ultrasound images and radiofrequency data.

Eur Radiol 2021 Jan 6. Epub 2021 Jan 6.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Department of Ultrasound, Sun Yat-sen University Cancer Center, 651 Dongfeng East Road, Guangzhou, 510060, Guangdong, People's Republic of China.

Objective: To develop a deep learning-based method with information fusion of US images and RF signals for better classification of thyroid nodules (TNs).

Methods: One hundred sixty-three pairs of US images and RF signals of TNs from a cohort of adult patients were used for analysis. We developed an information fusion-based joint convolutional neural network (IF-JCNN) for the differential diagnosis of malignant and benign TNs. The IF-JCNN contains two branched CNNs for deep feature extraction: one for US images and the other one for RF signals. The extracted features are fused at the backend of IF-JCNN for TN classification.

Results: Across 5-fold cross-validation, the accuracy, sensitivity, specificity, and area under the receiver operating characteristic curve (AUROC) obtained by using the IF-JCNN with both US images and RF signals as inputs for TN classification were respectively 0.896 (95% CI 0.838-0.938), 0.885 (95% CI 0.804-0.941), 0.910 (95% CI 0.815-0.966), and 0.956 (95% CI 0.926-0.987), which were better than those obtained by using only US images: 0.822 (0.755-0.878; p = 0.0044), 0.792 (0.679-0.868, p = 0.0091), 0.866 (0.760-0.937, p = 0.197), and 0.901 (0.855-0.948, p = .0398), or RF signals: 0.767 (0.694-0.829, p < 0.001), 0.781 (0.685-0.859, p = 0.0037), 0.746 (0.625-0.845, p < 0.001), 0.845 (0.786-0.903, p < 0.001).

Conclusions: The proposed IF-JCNN model filled the gap of just using US images in CNNs to characterize TNs, and it may serve as a promising tool for assisting the diagnosis of thyroid cancer.

Key Points: • Raw radiofrequency signals before ultrasound imaging of thyroid nodules provide useful information that is not carried by ultrasound images. • The information carried by raw radiofrequency signals and ultrasound images for thyroid nodules is complementary. • The performance of deep convolutional neural network for diagnosing thyroid nodules can be significantly improved by fusing US images and RF signals in the model as compared with just using US images.
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http://dx.doi.org/10.1007/s00330-020-07585-zDOI Listing
January 2021

NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.

JCI Insight 2021 Feb 8;6(3). Epub 2021 Feb 8.

Department of Foundations of Medicine, New York University Long Island School of Medicine, Mineola, New York, USA.

Infantile hemangioma is a vascular tumor characterized by the rapid growth of disorganized blood vessels followed by slow spontaneous involution. The underlying molecular mechanisms that regulate hemangioma proliferation and involution still are not well elucidated. Our previous studies reported that NOGOB receptor (NGBR), a transmembrane protein, is required for the translocation of prenylated RAS from the cytosol to the plasma membrane and promotes RAS activation. Here, we show that NGBR was highly expressed in the proliferating phase of infantile hemangioma, but its expression decreased in the involuting phase, suggesting that NGBR may have been involved in regulating the growth of proliferating hemangioma. Moreover, we demonstrate that NGBR knockdown in hemangioma stem cells (HemSCs) attenuated growth factor-stimulated RAS activation and diminished the migration and proliferation of HemSCs, which is consistent with the effects of RAS knockdown in HemSCs. In vivo differentiation assay further shows that NGBR knockdown inhibited blood vessel formation and adipocyte differentiation of HemSCs in immunodeficient mice. Our data suggest that NGBR served as a RAS modulator in controlling the growth and differentiation of HemSCs.
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http://dx.doi.org/10.1172/jci.insight.142299DOI Listing
February 2021

Diversity and Geographic Distribution of Microsymbionts Associated With Invasive Species in Southern China.

Front Microbiol 2020 28;11:563389. Epub 2020 Oct 28.

Institute of Agro-resources and Environment, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, China.

In order to investigated diversity and geographic distribitution of rhizobia associated with invasive species, nodules and soils around the plants were sampled from five provinces in southern China. In total, 361 isolates were obtained from and in 25 locations. A multi-locus sequence analysis (MLSA) including 16S rRNA, , , , , and identified the isolates into eight genospecies corresponding to , , , , sp., , , and . The majority of the isolates were (62.6%), followed by (33.5%) and (2.9%). strains were more predominant in nodules of (76.2) than in (59.9%), and the distribution of in those two plant species was reverse (3.4:18.2%). Four symbiotypes were defined among the isolates based upon the phylogeny of - genes, represented by , -, spp., and spp. The species affiliation and the symbiotype division among the isolates demonstrated the multiple origins of rhizobia in China: most were similar to those found in the original centers of plants, but sp. might have a local origin. The unbalanced distribution of symbionts between the two species might be related to the soil pH, organic matter and available nitrogen; spp. generally dominated most of the soils colonized by in this study, but it had a particular preference for neutral-alkaline soils with low fertility whereas. While spp. preferred more acidic and fertile soils. The spp. tended to prefer neutral-acidic soils with high fertility soils.
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http://dx.doi.org/10.3389/fmicb.2020.563389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673401PMC
October 2020

Codon optimization with deep learning to enhance protein expression.

Sci Rep 2020 10 19;10(1):17617. Epub 2020 Oct 19.

Chengdu Institute of Computer Applications, Chinese Academy of Sciences, Chengdu, 610041, China.

Heterologous expression is the main approach for recombinant protein production ingenetic synthesis, for which codon optimization is necessary. The existing optimization methods are based on biological indexes. In this paper, we propose a novel codon optimization method based on deep learning. First, we introduce the concept of codon boxes, via which DNA sequences can be recoded into codon box sequences while ignoring the order of bases. Then, the problem of codon optimization can be converted to sequence annotation of corresponding amino acids with codon boxes. The codon optimization models for Escherichia Coli were trained by the Bidirectional Long-Short-Term Memory Conditional Random Field. Theoretically, deep learning is a good method to obtain the distribution characteristics of DNA. In addition to the comparison of the codon adaptation index, protein expression experiments for plasmodium falciparum candidate vaccine and polymerase acidic protein were implemented for comparison with the original sequences and the optimized sequences from Genewiz and ThermoFisher. The results show that our method for enhancing protein expression is efficient and competitive.
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http://dx.doi.org/10.1038/s41598-020-74091-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7572362PMC
October 2020

Characterization of UMi028-A-1 stem cell line that contains a CRISPR/Cas9 induced hearing loss-associated variant (V60L (c.178G > T)) in the P2RX2 gene.

Stem Cell Res 2020 12 1;49:102017. Epub 2020 Oct 1.

Department of Otolaryngology, University of Miami Miller School of Medicine, Miami, FL 33136, United States; John P. Hussman Institute for Human Genomics; John T Macdonald Foundation Department of Human Genetics, University of Miami Miller School of Medicine, Miami, FL 33136, United States. Electronic address:

UMi028-A-1 hiPSC line contains a CRISPR/Cas9-induced heterozygous, hearing loss-associated variant (V60L (GTA > TTA)) in the Purinergic Receptor P2X2 (P2RX2) gene. This line, derived from an unaffected male iPSC line, has been successfully characterized for its cellular and genetic properties. The c.178G > T variant in P2RX2 is associated with non-syndromic, dominant, progressive hearing loss. Once differentiated into inner ear cell types, UMi028-A-1 will serve as a resource for understanding the molecular mechanisms underlying hearing loss and serve as a potential platform for testing therapeutic approaches to restore inner ear function.
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http://dx.doi.org/10.1016/j.scr.2020.102017DOI Listing
December 2020

Polycyclic aromatic hydrocarbons in particulate matter and serum club cell secretory protein change among schoolchildren: A molecular epidemiology study.

Environ Res 2021 Jan 8;192:110300. Epub 2020 Oct 8.

Jinan Municipal Center for Disease Control and Prevention, Jinan, China. Electronic address:

Airborne particulate matter (PM) is a complex mixture containing various kinds of harmful components. Exposure to air PM is associated with childhood respiratory disease, but epidemiological data are limited concerning the circulating respiratory injury protein on the etiology of childhood respiratory disease. Specifically, the role of PM toxic components or its biological effective dose (adduct) in respiratory injury remains unclear. To demonstrate the dose-response relationship and the main mechanism on circulating club cell secretory protein (CC16) from PM compositions among children, we enrolled 273 boarding schoolchildren in China, including 110 and 163 children of whom were in the low- and high-PM exposed areas, respectively. In this study, we measured the internal exposure levels, including serum polycyclic aromatic hydrocarbons (PAH) adduct, urinary metals, and AhR expression, and detected the serum CC16 level as a lung injury marker. Environmental tobacco exposure in children was assessed by urinary cotinine. We found that significantly higher levels of serum CC16, benzo[a]pyridin-7,8-dihydroglycol-9,10-epoxide (BPDE)-albumin adduct, urinary molybdenum, selenium, arsenic, cadmium and barium, and lower level of AhR expression in high-PM exposed group. There was a good association between serum BPDE-albumin adduct and CC16 (β = 0.222, P = 0.006). There was no association on urinary metals and serum CC16. BPDE-albumin adduct was directly associated with serum CC16 alternation [direct effect = 0.2044, 95% confidence interval (CI) = (0.0426, 0.36)]. PM could cause serum CC16 increased in children. PAH and its adduct might play a key role in lung injury during PM exposure.
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http://dx.doi.org/10.1016/j.envres.2020.110300DOI Listing
January 2021

The adsorption behavior of lithium on spinel titanium oxide nanosheets with exposed (1-14) high-index facets.

Dalton Trans 2020 Oct;49(40):14180-14190

Key Laboratory of Comprehensive and Highly Efficient Utilization of Salt Lake Resources, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences, Xining 810008, China. and Key Laboratory of Salt Lake Resources Chemistry of Qinghai Province, Xining 810008, China.

The ion-exchange process is usually influenced by the surface properties of the adsorbents. In particular, the prophase adsorption/desorption process is confined by different crystal facets. In this research, spinel Li4Ti5O12 nanosheets with an exposed (1-14) high-index facet were prepared by a hydrothermal method followed by calcination treatment. Then, a H4Ti5O12 adsorbent was obtained, covered with the same (1-14) facets, after treatment with 0.2 M HCl. This special facet-exposed H4Ti5O12 has high cycling ability, with the adsorption uptake remaining at 96.84% after four cycles, a fast adsorption equilibrium time (equilibrium time < 60 min), excellent ion adsorption selectivity for Li+ uptake (separation factor: Li+ > K+ > Ca2+ > Na+ > Mg2+), and good adsorption capacity for Li+ uptake (21.57 mg g-1 ). With the help of X-ray photoelectron spectroscopy analyses, the Li+ adsorption process on the H4Ti5O12 nanosheets is shown to be an ion-exchange process. In addition, the coordination relationship between lithium and oxygen ions was investigated, illustrating that the four-coordinated structure is more stable than other complexes. These results indicate that hydrogen ions are exchanged for lithium ions at tetrahedral 8a sites, leading to the H4Ti5O12 structure with high stability in the adsorption-desorption cycling process.
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http://dx.doi.org/10.1039/d0dt02960bDOI Listing
October 2020

Impact of perioperative allogeneic blood transfusion on the long-term prognosis of patients with different stage tumors after radical resection for hepatocellular carcinoma.

Eur J Surg Oncol 2021 Mar 21;47(3 Pt B):620-627. Epub 2020 Sep 21.

Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, Sichuan, China. Electronic address:

Background: We previously reported that perioperative allogeneic blood transfusion (PABT) did not affect long-term survival after radical resection for hepatocellular carcinoma (HCC). This study aimed to investigate the effects of PABT on the prognosis of HCC patients with different stage tumors.

Methods: Patients with primary HCC who underwent curative liver resection between 2003 and 2012 were retrospectively enrolled and divided into the early-stage (stage I) and non-early-stage (stages II, III and IV) groups. The impacts of PABT on the long-term prognosis of patients in different groups after resection were investigated using propensity score matching (PSM) and multivariable Cox regression analyses.

Results: We enrolled 426 HCC patients, including 53 matched pairs of patients with early-stage tumors and 51 matched pairs of patients with non-early-stage tumors. Survival analyses of the patients with early-stage tumors showed that the recurrence-free survival (RFS) and overall survival (OS) rates of the transfusion group were significantly worse than those of the nontransfusion group both before and after PSM. Multivariable Cox analyses identified that PABT was an independent predictor of RFS and OS of the patients with early-stage tumors. However, survival analyses of the propensity-matched patients with non-early-stage tumors showed no significant differences in RFS and OS rates between the transfusion and nontransfusion groups (p = 0.296; p = 0.472).

Conclusions: This study demonstrates that PABT has negative impacts on the long-term prognosis of patients with early-stage tumors after radical resection of HCC but has no impact on the long-term prognosis of patients with non-early-stage tumors.
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http://dx.doi.org/10.1016/j.ejso.2020.09.021DOI Listing
March 2021

LAMA4 upregulation is associated with high liver metastasis potential and poor survival outcome of Pancreatic Cancer.

Theranostics 2020 13;10(22):10274-10289. Epub 2020 Aug 13.

Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.

Pancreatic cancer is one of the most difficult cancers to manage and its poor prognosis stems from the lack of a reliable early disease biomarker coupled with its highly metastatic potential. Liver metastasis accounts for the high mortality rate in pancreatic cancer. Therefore, a better understanding of the mechanism(s) underlying the acquisition of the metastatic potential in pancreatic cancer is highly desirable. Microarray analysis in wild-type and highly liver metastatic human pancreatic cancer cell lines was performed to identify gene expression signatures that underlie the metastatic process. We validated our findings in patient samples, nude mice, cell lines and database analysis. We identified a metastasis-related gene, laminin subunit alpha 4 (), that was upregulated in highly liver metastatic human pancreatic cancer cell lines. Downregulation of LAMA4 reduced the liver metastatic ability of pancreatic cancer cells . Furthermore, LAMA4 expression was positively correlated with tumor severity and analyses revealed that LAMA4 was associated with altered tumor microenvironment. In particular, our and i results showed that LAMA4 expression was highly correlated with cancer-associated fibroblasts (CAFs) level which may contribute to pancreatic cancer metastasis. We further found that LAMA4 had a positive effect on the recruitment and activity of CAFs. These data provide evidence for LAMA4 as a possible biomarker of disease progression and poor prognosis in pancreatic cancer. Our findings indicate that LAMA4 may contribute to pancreatic cancer metastasis via recruitment or activation of CAFs.
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http://dx.doi.org/10.7150/thno.47001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481422PMC
August 2020

Effects of riboflavin and ultraviolet light treatment on pathogen reduction and platelets.

Transfusion 2020 Nov 31;60(11):2647-2654. Epub 2020 Aug 31.

Clinical Transfusion Research Center, Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China.

Background: Pathogen reduction technology has become an accepted method for limiting transfusion-transmitted infections (TTIs). Riboflavin and ultraviolet light (RUV) treatment of platelets (PLTs) is an optional means of pathogen inactivation owing to its safety, effectiveness, and ease of use. However, the literature on effects of ultraviolet (UV) light spectra and doses on pathogen reduction is still contradictory.

Methods: We tested the effectiveness of killing Escherichia coli following RUV exposure with one broad-spectrum and two narrow-spectrum light sources centered at 311 and 365 nm and at successively higher doses by limited dilution survival assays. After comparing the effectiveness of E coli and phage inactivation (n = 6) and the changes in PLT count and metabolism caused by RUV treatment with optimized UV light at increasing doses, we confirmed our results by using four model virus systems that represent common TTIs, as well as PLT function and activation assays at an optimized light dose.

Results: The narrow-spectrum UV, centered at 311 nm, optimally reduced the E coli titer with a light dose ≥8.11 J/mL, resulting in the same trend of E coli and phage reduction at different light doses. At 8.11 J/mL, 311-nm narrow-spectrum UV had a good inactivation effect on E coli and phages, eliminating many viruses, and resulted in acceptable PLT quality after RUV treatment and during storage for 4 days.

Conclusions: Our data suggest restricting exposure to narrow-spectrum UV centered at 311 nm can increase E coli elimination and potentially optimize virus titer reduction without significantly compromising PLT quality.
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http://dx.doi.org/10.1111/trf.16053DOI Listing
November 2020

Amorphous CrWO-Modified WO Nanowires with a Large Specific Surface Area and Rich Lewis Acid Sites: A Highly Efficient Catalyst for Oxidative Desulfurization.

ACS Appl Mater Interfaces 2020 Aug 12;12(34):38140-38152. Epub 2020 Aug 12.

State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, China.

The oxidative desulfurization (ODS) of fuel oils is of great significance for environmental protection, and the development of efficient ODS heterogeneous catalysts is highly desired. Herein, we have designed and synthesized a novel material of amorphous CrWO-modified WO (a-CrWO/WO) nanowires (3-6 nm) with a large specific surface area of 289.5 m·g and rich Lewis acid sites. The formation of such a unique nanowire is attributed to the adsorption of Cr cations on non-(001) planes of WO. In the ODS process, the a-CrWO/WO nanowires can efficiently oxidize benzothiophene (BT), dibenzothiophene (DBT), and 4,6-dimethyldibenzothiophene (4,6-DMDBT) to their corresponding sulfones in a quasi-microemulsion reaction system and possess the highest activity ( = 55.4 kJ/mol) for DBT: 99.0% of 15,000 ppm DBT with 2600 ppm S can be removed (70 °C, HO as the oxidant). The improvement in ODS activity from most of WO catalysts is owing to the sufficient active sites and enhanced adsorption of DBT on the basis of structural features of a-CrWO/WO nanowires. Combined with free radical capture experiments, a possible ODS mechanism of W(O) peroxotungstate route based on surface -OH groups is reasonably proposed. Moreover, the a-CrWO/WO nanowires have good stability and can be synthesized on a large scale, suggesting its potential applications as an efficient heterogeneous catalyst.
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http://dx.doi.org/10.1021/acsami.0c10118DOI Listing
August 2020

An Augmented Model with Inferred Blood Features for the Self-diagnosis of Metabolic Syndrome.

Methods Inf Med 2020 02 24;59(1):18-30. Epub 2020 Aug 24.

Health Management Center, The First Affiliated Hospital, Medical School of Zhejiang University, Hangzhou, People's Republic of China.

Background And Objectives: The penetration rate of physical examinations in China is substantially lower than that in developed countries. Therefore, an auxiliary approach that does not depend on hospital health checks for the diagnosis of metabolic syndrome (MetS) is needed.

Methods: In this study, we proposed an augmented method with inferred blood features that uses self-care inputs available at home for the auxiliary diagnosis of MetS. The dataset used for modeling contained data on 91,420 individuals who had at least 2 consecutive years of health checks. We trained three separate models using a regularized gradient-boosted decision tree. The first model used only home-based features; additional blood test data (including triglyceride [TG] data, fasting blood glucose data, and high-density lipoprotein cholesterol [HDL-C] data) were included in the second model. However, in the augmented approach, the blood test data were manipulated using multivariate imputation by chained equations prior to inclusion in the third model. The performance of the three models for MetS auxiliary diagnosis was then quantitatively compared.

Results: The results showed that the third model exhibited the highest classification accuracy for MetS in comparison with the other two models (area under the curve [AUC]: 3rd vs. 2nd vs. 1st = 0.971 vs. 0.950 vs. 0.905,  < 0.001). We further revealed that with full sets of the three measurements from earlier blood test data, the classification accuracy of MetS can be further improved (AUC: without vs. with = 0.971 vs. 0.993). However, the magnitude of improvement was not statistically significant at the 1% level of significance ( = 0.014).

Conclusion: Our findings demonstrate the feasibility of the third model for MetS homecare applications and lend novel insights into innovative research on the health management of MetS. Further validation and implementation of our proposed model might improve quality of life and ultimately benefit the general population.
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http://dx.doi.org/10.1055/s-0040-1710382DOI Listing
February 2020

[Analysis on Reentry Situation of HBsAg Single Reagent Reactive Blood Donors in Anhui Province].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2020 Aug;28(4):1391-1396

Department of Microbiology and Parasitology, Basic Medicine College, Anhui Medical University, Hefei 230032, Anhui Province, China,E-mail:

Objective: To analyze the reentry situation of HBsAg single reagent reactive blood donors in Anhui province, and to verify the rationality and effectiveness of reentry strategy of blood donors in Anhui province.

Methods: Shielded blood donors who were HBsAg single reagent reactive might voluntarily apply for returning to the team of blood donors after the shield of 6 months. Blood bankstaff that shielded those donors should draw blood and conduct screening tests. Samples from donors who were HBsAg negative should be delivered to Anhui Blood Center to conduct the reentry detections. Shielded blood donors were allowed to return to the team if the results of HBsAg test, neutralization test, HBcAb test and nucleic acid test were negative.

Results: 109 person-portions of samples for returning to team from September 2013 to December 2016 were delivered to Anhui Blood Center. After reentry tests, 60 of them were negative, 8 cases were positive, while 41 cases were undetermined, and the qualified rate was 55.05%.25 negative donors were from Hefei, 20 of them donated blood again and were negative.

Conclusion: The shielding and reentry strategy of blood donors with HBsAg single reagent reactive in Anhui province is rational and effective. However, there are still some deficiencies in trace of donors and information transmission, which needs to be further improved.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2020.04.053DOI Listing
August 2020

[LVIS stent-assisted coil embolization in the acute stage of ruptured intracranial aneurysms].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2020 Jul;32(7):828-834

Xiamen Medical Emergency Center, Xiamen 361021, Fujian, China. Corresponding author: Chen E, Email:

Objective: To evaluate the safety and efficacy of LVIS stent-assisted coil embolization in the acute phase of ruptured intracranial aneurysms.

Methods: The clinical data of 55 patients with ruptured intracranial aneurysm treated with LVIS stent-assisted coil embolization admitted to Zhongshan Hospital of Xiamen University from January 2016 to December 2018 were analyzed retrospectively. The general data, the characteristics of aneurysms and the occurrence of perioperative complications of the patients were collected. The clinical prognosis of the patients at discharge and 6 months of follow-up was recorded. The Glasgow prognosis score (GOS) was graded as good (5), average (3-4), and poor (1-2), and the cerebral angiography results were recorded immediately after embolization and 6-month follow-up. The aneurysm occlusion was assessed by Raymond grade, Raymond I was complete obliteration, II was residual neck and III was residual aneurysm.

Results: All 55 patients received LVIS stent-assisted coil embolization within 72 hours of ruptured intracranial aneurysms, and all stents were released successfully, including 16 males (29.1%) and 39 females (70.9%). The median age was 53 (24-80) years old. Anterior circulation aneurysms were found in 49 patients (89.1%) and posterior circulation aneurysms in 6 patients (10.9%). According to Hunt-Hess classification, there were 43 patients with grade I-II (78.2%), 7 patients with grade III (12.7%) and 5 patients with grade IV-V (9.1%). The first digital subtraction angiography (DSA) examination of 55 patients after embolization showed that 41 patients had complete obliteration of aneurysms and 14 had residual neck; and the smaller the aneurysm was, the higher the rate of complete obliteration after embolization was. The proportion of small aneurysms (maximum diameter ≤ 7 mm) in the complete obliteration group was significantly higher than that in the neck residual group (100.0% vs. 64.3%, P < 0.01). Among the 55 patients, there was 1 patient suffered from in-stent thrombosis during embolization, 1 patient suffered from distal vascular thrombosis induced by plaque shedding during embolization, 1 patient suffered from vasospasm during embolization, and 1 patient suffered from postoperative distal cerebral hemorrhage after embolization. In 2 dead patients, 1 died of cardiogenic disease and 1 died of respiratory failure caused by severe pneumonia. At discharge, the prognosis was good in 40 patients, average in 10 patients, and poor in 5 patients; and the higher the Hunt-Hess grade at admission, the worse the prognosis. The proportion of patients with Hunt-Hess grade I-II at admission in the good prognosis group was significantly higher than that in the general prognosis group and the poor prognosis group (90.0% vs. 50.0%, 40.0%, P < 0.01). Of the 55 patients, 39 completed clinical prognosis and cerebral angiography 6 months after embolization for follow-up. All patients had GOS no less than 3, including 32 patients with complete obliteration of aneurysm, 4 with residual neck and 3 with residual aneurysm. The smaller the aneurysm, the higher the rate of complete obliteration at 6-month follow-up was. The proportion of small aneurysm in the complete obliteration group was significantly higher than that in the residual neck group and the residual aneurysm group (100.0% vs. 75.0%, 33.3%, P < 0.01). There was no rebleeding or ischemic complication at 6-month follow-up.

Conclusions: LVIS stent assisted coil embolization is safe, effective and feasible in the acute stage of ruptured intracranial aneurysms. Standardizing antiplatelet therapy and dense packing of aneurysms during embolization are the key to reduce bleeding and ischemic complications.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200514-00389DOI Listing
July 2020

Errors in Trial of Effect of Convalescent Plasma Therapy on Time to Clinical Improvement in Patients With Severe and Life-threatening COVID-19.

Authors:
Zhong Liu

JAMA 2020 08;324(5):518-519

Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China.

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http://dx.doi.org/10.1001/jama.2020.12607DOI Listing
August 2020

Integration of quantitative proteomics and metabolomics reveals tissue hypoxia mechanisms in an ischemic-hypoxic rat model.

J Proteomics 2020 09 28;228:103924. Epub 2020 Jul 28.

Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu 610052, China; Key Laboratory of Transfusion Adverse Reactions, Chinese Academy of Medical Sciences, Chengdu 610052, China. Electronic address:

Tissues hypoxia caused by hemorrhage is a common complication in many clinical diseases. However, its pathological mechanism remains largely unknown. To partly address this issue, an ischemic-hypoxic rat model was established and the plasma proteomic and metabolic profiles were quantified and analyzed using TMT-based quantitative proteomics and metabolomics. The analysis revealed a total of 177 differentially expressed proteins and 32 metabolites that were uniquely altered in the hypoxic rat plasma, compared to the control. Bioinformatics analysis showed that these altered proteins and metabolites were involved in a wide range of biological processes. Twelve of the 177 differentially expressed proteins were involved in PI3K-Akt signaling, a pathway that has been reported to be strongly associated with tissue hypoxia. Other signaling pathways such as complement and coagulation cascades, GnRH signaling, relaxin signaling, protein processing in endoplasmic reticulum, as well as AGE-RAGE signaling were markedly altered in the ischemic-hypoxic response, implying their potential roles in tissue hypoxia. A joint analysis of proteome and metabolome showed that the significantly altered metabolites such as guanine, tryptamine, dopamine, hexadecenoic, l-methionine, and fumarate may have participated in the pathogenesis of tissue hypoxia. Further, we found that changes in the levels of metabolites matched the changes in protein abundance within the same pathway. Overall, this study presents an overview of the molecular networks in ischemic-hypoxic pathology and offers biochemical basis for further study on the mechanism of tissue hypoxia. SIGNIFICANCE: We employed an integrated metabonomic-proteomic method to systematically analyze the profiles of metabolites and proteins in an ischemic-hypoxic rat model. Bioinformatics and enrichment analysis showed that the differentially expressed proteins were mainly involved in complement and coagulation cascades, PI3K-Akt signaling, GnRH signaling, relaxin signaling, protein processing in endoplasmic reticulum, and AGE-RAGE signaling. Moreover, a panel of 12 candidate proteins involved in PI3K-Akt signaling (i.e., Vtn, Hsp90b1, Ywhae, Tnc, Ywhaz, Thbs4, Lamc1, Col1a1, Il2rg, Egfr, Newgene 621,351, and Tfrc) may serve as the potential biomarkers to predict tissue hypoxia.
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http://dx.doi.org/10.1016/j.jprot.2020.103924DOI Listing
September 2020

K-gradient doping to stabilize the spinel structure of LiMnO for Li recovery.

Dalton Trans 2020 Aug;49(31):10939-10948

Key Laboratory of Comprehensive and Highly Efficient Utilization of Salt Lake Resources, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences, Xining 810008, China. and Key Laboratory of Salt Lake Resources Chemistry of Qinghai Province, Xining 810008, China.

Lithium-rich spinel lithium manganese oxide (LMO) compounds are one kind of promising adsorbents for lithium recovery from brine due to their high capacity and low Mn dissolution; Li1.6Mn1.6O4 is one of them. However, Mn3+ exists in the Li1.6Mn1.6O4 precursor due to incomplete reaction during syntheses, and the disproportionation reaction of Mn3+ inevitably results in Mn dissolution during lithium adsorption and desorption. The stable recycling and structural stability of Li1.6Mn1.6O4 were improved in aqueous lithium resources through K-gradient doping (LMO-K). The dissolution of Mn is reduced to 4.0% from 5.4% (before doping) and the adsorption capacity is kept at high capacity (31.6 mg g-1) at a low Li+ concentration of 12 mmol L-1. In addition, first-principles calculations further confirm that K substitutes for Li at 16d sites, leading to the stabilization of the Mn cations in the compound. With the help of K doping, the undesired dissolution of Mn in the cycle process is inhibited, which may be due to the reduction in the content of Mn3+ and improvement in the structural stability of the adsorbent.
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http://dx.doi.org/10.1039/d0dt02405hDOI Listing
August 2020

Inhibitory Effect of PIK-24 on Respiratory Syncytial Virus Entry by Blocking Phosphatidylinositol-3 Kinase Signaling.

Antimicrob Agents Chemother 2020 09 21;64(10). Epub 2020 Sep 21.

Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou, Guangdong, China

Phosphoinositide-3 kinase signaling modulates many cellular processes, including cell survival, proliferation, differentiation, and apoptosis. Currently, it is known that the establishment of respiratory syncytial virus infection requires phosphoinositide-3 kinase signaling. However, the regulatory pattern of phosphoinositide-3 kinase signaling or its corresponding molecular mechanism during respiratory syncytial virus entry remains unclear. Here, the involvement of phosphoinositide-3 kinase signaling in respiratory syncytial virus entry was studied. PIK-24, a novel compound designed with phosphoinositide-3 kinase as a target, had potent anti-respiratory syncytial virus activity both and PIK-24 significantly reduced viral entry into the host cell through blocking the late stage of the fusion process. In a mouse model, PIK-24 effectively reduced the viral load and alleviated inflammation in lung tissue. Subsequent studies on the antiviral mechanism of PIK-24 revealed that viral entry was accompanied by phosphoinositide-3 kinase signaling activation, downstream RhoA and cofilin upregulation, and actin cytoskeleton rearrangement. PIK-24 treatment significantly reversed all these effects. The disruption of actin cytoskeleton dynamics or the modulation of phosphoinositide-3 kinase activity by knockdown also affected viral entry efficacy. Altogether, it is reasonable to conclude that the antiviral activity of PIK-24 depends on the phosphoinositide-3 kinase signaling and that the use of phosphoinositide-3 kinase signaling to regulate actin cytoskeleton rearrangement plays a key role in respiratory syncytial virus entry.
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http://dx.doi.org/10.1128/AAC.00608-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508614PMC
September 2020

Characteristics and serological patterns of COVID-19 convalescent plasma donors: optimal donors and timing of donation.

Transfusion 2020 08 6;60(8):1765-1772. Epub 2020 Jul 6.

Clinical Transfusion Research Center, Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, Sichuan, China.

Background: The lack of effective treatments against the 2019 coronavirus disease (COVID-19) has led to the exploratory use of convalescent plasma for treating COVID-19. Case reports and case series have shown encouraging results. This study investigated SARS-CoV-2 antibodies and epidemiological characteristics in convalescent plasma donors, to identify criteria for donor selection.

Methods: Recovered COVID-19 patients, aged 18-55 years, who had experienced no symptoms for more than 2 weeks, were recruited. Donor characteristics such as disease presentations were collected and SARS-CoV-2 N-specific IgM, IgG, and S-RBD-specific IgG levels were measured by enzyme-linked immunosorbent assay (ELISA).

Results: Whereas levels of N-specific IgM antibody declined after recovery, S-RBD-specific and N-specific IgG antibodies increased after 4 weeks from the onset of symptoms, with no significant correlation to age, sex, or ABO blood type. Donors with the disease presentation of fever exceeding 38.5°C or lasting longer than 3 days exhibited higher levels of S-RBD-specific IgG antibodies at the time of donation. Of the 49 convalescent plasma donors, 90% had an S-RBD-specific IgG titer of ≥1:160 and 78% had a titer of ≥1:640 at the time of plasma donation. Of the 30 convalescent plasma donors, who had donated plasma later than 28 days after the onset of symptoms and had a disease presentation of fever lasting longer than 3 days or a body temperature exceeding 38.5°C, 100% had an S-RBD-specific IgG titer of ≥1:160 and 93% had a titer of ≥1:640.

Conclusion: This study indicates that the S-RBD-specific IgG antibody reaches higher levels after 4 weeks from the onset of COVID-19 symptoms. We recommend the following selection criteria for optimal donation of COVID-19 convalescent plasma: 28 days after the onset of symptoms and with a disease presentation of fever lasting longer than 3 days or a body temperature exceeding 38.5°C. Selection based on these criteria can ensure a high likelihood of achieving sufficiently high S-RBD-specific IgG titers.
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http://dx.doi.org/10.1111/trf.15918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7361741PMC
August 2020

[Establishing the Blood Donor Deferral Criterion in TP ELISA Test].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2020 Jun;28(3):956-960

Clinical Transfusion Research Center,Institute of Blood Transfusion,Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Transfusion Adverse Reactions Chinese Academy of Medical Sciences, Chengdu 610081, Sichuan Province,

Objective: To Establish the shielding threshold value of TP antibody ELISA for unpaid blood donors, so as to shield true positive blood donors from returning to team management.

Methods: The real serological status of 517 samples with anti-TP ELISA reactivity was determined by confirmation test of Treponema pallidum particle agglutination (TPPA). The shielding threshold of TP antibody was preliminarily determined by using 99% specificity of ROC and 95% positive predictive value of percentile method, respectively. 283 TP antibody reactivity specimens routinely tested in our laboratory were selected to determine the applicability of the initial shielding values obtained by the two methods, and finally to determine the shielding threshold values of TP antibody donors.

Results: The specific S/CO values of reagent A 99% were 13.33-16.18, that of reagent B 99% was 6.34, that of reagent B 99% was 13.17-19.85, and that of 95% was 6.62. Empirical evidence: 99% specific threshold shielding true positive rates of reagents A and B were 100%, 95% positive expected value shielding true positive rates were 98.4%, 99%. Final determination of 99% specific shielding threshold as a low value of blood donors shielding threshold. The shielding limits of reagent A and B were 13.33 and 13.17.

Conclusion: The shielding threshold of TP antibody ELISA for blood donors established in this study can help to reduce the number of blood donors returning to team management.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2020.03.039DOI Listing
June 2020

A genetic polymorphism of IL17F rs763780 associated with anti-E production in the Han Chinese population.

Transfus Apher Sci 2020 Aug 27;59(4):102745. Epub 2020 Feb 27.

Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China; Key Laboratory of Transfusion Adverse Reactions, Chinese Academy of Medical Sciences, Chengdu, China. Electronic address:

Objective: This study aimed to investigate the association among 4 single nucleotide polymorphisms (SNPs) in the genes TLR3, IL17F, ERAP1 and ERAP2 with anti-E alloantibody production.

Background: Anti-E alloantibodies can lead to clinically significant delayed hemolytic transfusion reactions (DHTRs) and hemolytic disease of the newborn (HDN). Some individuals produce anti-E alloantibodies post- transfusion. The mechanisms controlling this process is poorly understood.

Methods: Ninety-five patients with anti-E alloantibodies were enrolled, and samples from 186 healthy donors were used as controls. Four SNPs in the immune-related genes (TLR3, IL17F, ERAP1 and ERAP2) were selected. SNPs were analyzed by polymerase chain reactions (PCR) and TaqMan assays. Allele and genotype frequencies were compared using Pearson's chi-square test.

Results: The C allele and CC + CT genotypes of rs763780 in the IL17F gene were overrepresented in the E- alloimmunized patient group (14.2 % vs. 5.1 %, P < 0.001; 23.2 % vs. 9.7 %; P = 0.004). Individuals with CC + CT genotypes of rs763780 had a higher risk of E-alloimmunization. (OR, 2.81; 95 % CI, 1.42-5.56). No significant difference was observed among the other 3 SNPs.

Conclusions: SNP rs763780 in the IL17F gene was associated with E-alloimmunization in a sample of the Han Chinese population, with the allele C as a risk allele.
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http://dx.doi.org/10.1016/j.transci.2020.102745DOI Listing
August 2020

Effect of Convalescent Plasma Therapy on Time to Clinical Improvement in Patients With Severe and Life-threatening COVID-19: A Randomized Clinical Trial.

JAMA 2020 08;324(5):460-470

Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China.

Importance: Convalescent plasma is a potential therapeutic option for patients with coronavirus disease 2019 (COVID-19), but further data from randomized clinical trials are needed.

Objective: To evaluate the efficacy and adverse effects of convalescent plasma therapy for patients with COVID-19.

Design, Setting, And Participants: Open-label, multicenter, randomized clinical trial performed in 7 medical centers in Wuhan, China, from February 14, 2020, to April 1, 2020, with final follow-up April 28, 2020. The trial included 103 participants with laboratory-confirmed COVID-19 that was severe (respiratory distress and/or hypoxemia) or life-threatening (shock, organ failure, or requiring mechanical ventilation). The trial was terminated early after 103 of a planned 200 patients were enrolled.

Intervention: Convalescent plasma in addition to standard treatment (n = 52) vs standard treatment alone (control) (n = 51), stratified by disease severity.

Main Outcomes And Measures: Primary outcome was time to clinical improvement within 28 days, defined as patient discharged alive or reduction of 2 points on a 6-point disease severity scale (ranging from 1 [discharge] to 6 [death]). Secondary outcomes included 28-day mortality, time to discharge, and the rate of viral polymerase chain reaction (PCR) results turned from positive at baseline to negative at up to 72 hours.

Results: Of 103 patients who were randomized (median age, 70 years; 60 [58.3%] male), 101 (98.1%) completed the trial. Clinical improvement occurred within 28 days in 51.9% (27/52) of the convalescent plasma group vs 43.1% (22/51) in the control group (difference, 8.8% [95% CI, -10.4% to 28.0%]; hazard ratio [HR], 1.40 [95% CI, 0.79-2.49]; P = .26). Among those with severe disease, the primary outcome occurred in 91.3% (21/23) of the convalescent plasma group vs 68.2% (15/22) of the control group (HR, 2.15 [95% CI, 1.07-4.32]; P = .03); among those with life-threatening disease the primary outcome occurred in 20.7% (6/29) of the convalescent plasma group vs 24.1% (7/29) of the control group (HR, 0.88 [95% CI, 0.30-2.63]; P = .83) (P for interaction = .17). There was no significant difference in 28-day mortality (15.7% vs 24.0%; OR, 0.59 [95% CI, 0.22-1.59]; P = .30) or time from randomization to discharge (51.0% vs 36.0% discharged by day 28; HR, 1.61 [95% CI, 0.88-2.95]; P = .12). Convalescent plasma treatment was associated with a negative conversion rate of viral PCR at 72 hours in 87.2% of the convalescent plasma group vs 37.5% of the control group (OR, 11.39 [95% CI, 3.91-33.18]; P < .001). Two patients in the convalescent plasma group experienced adverse events within hours after transfusion that improved with supportive care.

Conclusion And Relevance: Among patients with severe or life-threatening COVID-19, convalescent plasma therapy added to standard treatment, compared with standard treatment alone, did not result in a statistically significant improvement in time to clinical improvement within 28 days. Interpretation is limited by early termination of the trial, which may have been underpowered to detect a clinically important difference.

Trial Registration: Chinese Clinical Trial Registry: ChiCTR2000029757.
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http://dx.doi.org/10.1001/jama.2020.10044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7270883PMC
August 2020

Feasibility of a pilot program for COVID-19 convalescent plasma collection in Wuhan, China.

Transfusion 2020 08 31;60(8):1773-1777. Epub 2020 Jul 31.

Clinical Transfusion Research Center, Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, Sichuan, China.

Background: A novel coronavirus has caused an international outbreak. Currently, there are no specific therapeutic agents for coronavirus infections. Convalescent plasma (CP) therapy is a potentially effective treatment option.

Methods: Patients who had recovered from COVID-19 and had been discharged from the hospital for more than 2 weeks were recruited. COVID-19 convalescent plasma (CCP)-specific donor screening and selection were performed based on the following criteria: 1) aged 18-55 years; 2) eligible for blood donation; 3) diagnosed with COVID-19; 4) had two consecutive negative COVID-19 nasopharyngeal swab tests based on PCR (at least 24 hr apart) prior to hospital discharge; 5) had been discharged from the hospital for more than 2 weeks; and 6) had no COVID-19 symptoms prior to convalescent plasma donation. In addition, preference was given to CCP donors who had a fever lasting more than 3 days or a body temperature exceeding 38.5°C (101.3°F), and who donated 4 weeks after the onset of symptoms. CCP collection was performed using routine plasma collection procedures via plasmapheresis. In addition to routine donor testing, the CCP donors' plasma was also tested for SARS-CoV-2 nucleic acid and S-RBD-specific IgG antibody.

Results: Of the 81 potential CCP donors, 64 (79%) plasma products were collected. There were 18 female donors and 46 male donors. There were 34 first-time blood donors and 30 repeat donors. The average time between CCP collection and initial symptom onset was 49.1 days, and the average time between CCP collection and hospital discharge was 38.7 days. The average volume of CCP collected was 327.7 mL. All Alanine transaminase (ALT) testing results met blood donation requirements. HIV Ag/Ab, anti-HCV, anti-syphilis, and HBsAg were all negative; NAT for HIV, HBV, and HCV were also negative. In addition, all of the CCP donors' plasma units were negative for SARS-CoV-2 RNA. Of the total 64 CCP donors tested, only one had an S-RBD-specific IgG titer of 1:160, all others had a titer of ≥1:320.

Conclusion: Based on a feasibility study of a pilot CCP program in Wuhan, China, we demonstrated the success and feasibility of CCP collection. In addition, all of the CCP units collected had a titer of ≥1:160 for S-RBD-specific IgG antibody, which met the CCP quality control requirements based on the Chinese national guidelines for CCP.
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http://dx.doi.org/10.1111/trf.15921DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300543PMC
August 2020

[Remote sensing retrieval of maize residue cover on soil heterogeneous background].

Ying Yong Sheng Tai Xue Bao 2020 Feb;31(2):474-482

Institute of Remote Sensing Application, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.

Maize stalk mulching is a conservation tillage method that has been currently promoted in northeastern China Plain. Remote sensing estimation of regional crop residue cover (CRC) can quickly obtain the information of straw mulching in a large area, which plays an important role in monitoring and popularizing the work of straw mulching. In this study, the normalized difference til-lage index (NDTI), simple tillage index (STI), normalized difference residue index (NDRI), and normalized difference index 7 (NDI7) were extracted from Sentinel-2A image and used to establish a linear regression model for CRC and spectral indices in Lishu County of Jilin Province. The results showed that soils had strong spatial heterogeneity in the study area, which would lead to a significant impact on the spectral index regression model. Using soil texture classification (zoning) to establish regression model could improve the inversion accuracy. Soil spatial heterogeneity would increase the estimation error of the model. The four spectral indices had a strong correlation with CRC, among which the NDTI and STI models performed better. The zonal linear regression model based on NDTI and STI verified that R was 0.84 and RMSE was 13.3%, which was better than the non-zonal model (R was 0.75 and RMSE was 16.5%) and thus effectively improved the inversion accuracy.
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http://dx.doi.org/10.13287/j.1001-9332.202002.012DOI Listing
February 2020