Publications by authors named "Zhiyuan Yang"

75 Publications

Understanding the complex environmental management through a len of food-water-ecosystem nexus: Insights from an ecosystem restoration hotspot in dryland.

Sci Total Environ 2021 Apr 15;783:147029. Epub 2021 Apr 15.

Guangdong Open Laboratory of Geospatial Information Technology and Application, Guangzhou Institute of Geography, Guangdong Academy of Sciences, Guangzhou 510070, PR China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, PR China.

The Ecosystem Restoration Project (ERP) is a critical and urgent practice to achieve the land degradation neutrality (LDN) targets. However, an insufficient understanding of the balance between contrasting sectors of the food-water-ecosystem nexus results in ineffectiveness in supporting complex environmental management (CEM), leading to undesirable ERP failures. The Ordos Plateau case identified the nexus evolution and the non-linear interactions between sectors, which were expected to support adaptive strategy formulations for CEM and achieve win-win outcomes. Revegetation in drylands substantially boosted ecosystem restoration, alleviating soil erosion. However, the excessive reliance on returning cropland to woodland and grassland has caused a significant loss of arable and grazing land. During the initial period of ERPs, this exacerbated decline in grain and meat productivity. In addition, aggressive revegetation activities have also reduced runoff yield and depleted soil water resources. Water scarcity is recognized as the most challenging issue in dryland ecosystem restoration, heavily influencing the interactions between sectors and threatening the long-term sustainability of ERPs. To accommodate for regional water carrying capacity, ERPs should adopt and properly allocate the use of suitable plant species with a proven anti-drought capability and high survival ratios without additional human interventions. In addition, the evolution regimes, driving factors, critical thresholds, and complex feedbacks between the nexus sectors should be fully understood to address the water resources constraints and reconcile trade-offs. This would enable the prevention of ecosystem shifts to undesirable failures and inform timely and cost-effective CEM to achieve the LDN targets.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147029DOI Listing
April 2021

A Novel Strategy to Enhance the Performance of CO Adsorption Separation: Grafting Hyper-cross-linked Polyimide onto Composites of UiO-66-NH and GO.

ACS Appl Mater Interfaces 2021 Apr 7;13(15):17781-17790. Epub 2021 Apr 7.

College of Chemistry and Chemical Engineering, Xi'an University of Science and Technology, Xi'an, 710054, People's Republic of China.

Graphene oxide (GO) is widely used to improve the pore structure, dispersion capacity, adsorption selectivity, resistance to acids and bases, and thermal stability of metal-organic frameworks (MOFs). However, it remains a daunting challenge to enhance selectivity simply by modifying the pore surface polarity and producing a suitable pore structure for CO molecules through a combination of GO with MOFs. Herein, we demonstrate a novel porous hyper-cross-linked polyimide-UiO-graphene composite adsorbent for CO capture via in situ chemical knitting and condensation reactions. Specifically, a network of polyimides rich in carbonyl and nitrogen atoms with amino terminations was synthesized via the reaction of 4,4'-oxydiphthalic anhydride (ODPA) and 2,4,6-trimethyl-1,3-phenylenediamine (DAM). The product plays a crucial role in the separation of CO from N. As expected, the resulting composite (PI-UiO/GO-1) exhibited a 3-fold higher CO capacity (8.24 vs 2.8 mmol·g at 298 K and 30 bar), 4.2 times higher CO/N selectivity (64.71 vs 15.43), and significantly improved acid-base resistance stability compared with those values of pristine UiO-66-NH Furthermore, breakthrough experiments verified that the porous composites can effectively separate CO from simulated fuel gas (CO/N = 15/85 vol %) with great potential in industrial applications. More importantly, this strategy can be extended to prepare other MOF-based composites. This clearly advances the development of MOF-polymer materials for gas capture.
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http://dx.doi.org/10.1021/acsami.1c00917DOI Listing
April 2021

Win-win-win pathway for ecological restoration by balancing hydrological, ecological, and agricultural dimensions: Contrasting lessons from highly eroded agroforestry.

Sci Total Environ 2021 Jun 6;774:145140. Epub 2021 Feb 6.

Guangdong Open Laboratory of Geospatial Information Technology and Application, Guangzhou Institute of Geography, Guangdong Academy of Sciences, Guangzhou 510070, PR China; Key Lab of Guangdong for Utilization of Remote Sensing and Geographical Information System, Guangzhou 510070, PR China.

Ecological restoration projects (ERP) can effectively reverse ecosystem degradation. However, some ERPs have failed to restore ecosystems under environmental constraints, and they were unable to achieve the desired ecological and economic benefits. To achieve a win-win-win target that balances the hydrological, ecological, and agricultural dimensions, we introduced the contrasting lessons from hotspots of ecosystem restoration in the arid Loess Plateau (LP) and the humid Karst Plateau (KP) in China, and discussed a novel strategy for coordinating ecosystem restoration, water and food security, and residents' livelihoods. The biophysical models and related statistical records showed that aggressive ERPs and soil and water conservation projects (SWCPs) significantly promoted vegetation restoration and reduced soil erosion and sediment yield in both areas. However, excessive afforestation in the arid LP exhausted water resources and threatened ecosystem sustainability. The accelerated replacement of cropland since 1999 in the LP aggravated cropland shortage which led to carbon sequestration and grain productivity declines in the initial years. However, the construction of terrace and check-dam fields and improvements in the conditions of agricultural production reconciled the cropland shortage and stabilized food security. The positive involvement of stakeholders in ERPs effectively minimized land degradation through economic development and the improved livelihoods of local residents. Therefore, based on the evidence from the KP and LP, the proposed win-win-win strategy is potentially applicable in other global regions that suffer from land degradation. This strategy can achieve considerable success if the planners have a good understanding of local environmental conditions as well as the social and economic needs of residents affected by ERPs.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145140DOI Listing
June 2021

Effects of the entomopathogenic fungus Clonostachys rosea on mortality rates and gene expression profiles in Diaphorina citri adults.

J Invertebr Pathol 2021 Feb 25;179:107539. Epub 2021 Jan 25.

Guangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China. Electronic address:

Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), is a serious pest of citrus. The insect also transmits Candidatus Liberibacter asiaticus, the pathogen of a devastating citrus disease called Huanglongbing. Clonostachys rosea is a versatile fungus that possesses nematicidal and insecticidal activities. The effect of C. rosea against D. citri remains unclear. Here we examined the pathogenicity of C. rosea against D. citri adults. A mortality rate of 46.67% was observed in adults treated with 1 × 10 conidia/mL spore suspension. Comparative transcriptomic analyses identified 259 differentially-expressed genes (DEGs) between controls and samples treated with fungi. Among the DEGs, 183 were up-regulated and 76 down-regulated. Genes with altered expression included those involved in immunity, apoptosis and cuticle formation. Our preliminary observation indicated that C. rosea is virulent against ACP adults and has the potential as a biological control agent for ACP management in the field.
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http://dx.doi.org/10.1016/j.jip.2021.107539DOI Listing
February 2021

Transforming Metal-Organic Frameworks into Porous Liquids via a Covalent Linkage Strategy for CO Capture.

ACS Appl Mater Interfaces 2021 Jan 6;13(2):2600-2609. Epub 2021 Jan 6.

School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, Xi'an 710129, P. R. China.

Porous liquids (PLs), an emerging kind of liquid materials with permanent porosity, have attracted increasing attention in gas capture. However, directly turning metal-organic frameworks (MOFs) into PLs via a covalent linkage surface engineering strategy has not been reported. Additionally, challenges including reducing the cost and simplifying the preparation process are daunting. Herein, we proposed a general method to transform Universitetet i Oslo (UiO)-66-OH MOFs into PLs by surface engineering with organosilane (OS) and oligomer species via covalent bonding linkage. The oligomer species endow UiO-66-OH with superior fluidity at room temperature. Meanwhile, the resulting PLs showed great potential in both CO adsorption and CO/N selective separation. The residual porosity of PLs was verified by diverse characterizations and molecular simulations. Besides, CO selective capture sites were determined by grand canonical Monte Carlo (GCMC) simulation. Furthermore, the universality of the covalent linkage surface engineering strategy was confirmed using different classes of oligomer species and another MOF (ZIF-8-bearing amino groups). Notably, this strategy can be extended to construct other PLs by taking advantages of the rich library of oligomer species, thus making PLs promising candidates for further applications in energy and environment-related fields, such as gas capture, separation, and catalysis.
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http://dx.doi.org/10.1021/acsami.0c18707DOI Listing
January 2021

Molecular detection and genetic characterization of infectious laryngotracheitis virus in poultry in Myanmar.

BMC Vet Res 2020 Nov 23;16(1):453. Epub 2020 Nov 23.

Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

Background: Avian infectious laryngotracheitis (ILT) is a highly contagious viral disease that causes severe economic losses to the poultry industry worldwide. In Southeast Asian countries, including Myanmar, poultry farming is a major industry. Although it is known that infectious respiratory pathogens, including infectious laryngotracheitis virus (ILTV), are a major threat to poultry farms, there are no data currently available on the epidemiology of ILTV in Myanmar. Therefore, in this study, we conducted a molecular detection of ILTV in 20 poultry farms in Myanmar.

Results: Of the 57 tested oropharyngeal swabs, 10 were positive for ILTV by polymerase chain reaction of a 647 bp region of the thymidine kinase (TK) gene, giving a prevalence of ILTV of 17.5% (10/57). Further sequencing analysis of infected cell protein 4 (ICP4) gene and glycoprotein B, G, and J (gB, gG, and gJ) genes indicated that these isolates were field strains. Phylogenetic analysis revealed that the Myanmar strains clustered together in a single branch and were closely related to other reference strains isolated from Asian countries.

Conclusions: This study demonstrated the presence of ILTV in poultry farms in Myanmar. The genetic characterization analysis performed provides the fundamental data for epidemiological studies that monitor circulating strains of ILTV in Myanmar.
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http://dx.doi.org/10.1186/s12917-020-02666-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685572PMC
November 2020

Exploring soil erosion trajectories and their divergent responses to driving factors: a model-based contrasting study in highly eroded mountain areas.

Environ Sci Pollut Res Int 2021 Mar 20;28(12):14720-14738. Epub 2020 Nov 20.

Guangdong Open Laboratory of Geospatial Information Technology and Application, Guangzhou Institute of Geography, Guangdong Academy of Sciences, Guangzhou, 510070, People's Republic of China.

Soil erosion threatens environmental sustainability worldwide. Exploring the trajectories of soil erosion and associated drivers is of great significance for combating land degradation. This study selected the highly eroded Loess Plateau (LP) and Karst Plateau (KP) as contrasting regions to monitor soil erosion dynamics. Monitoring was performed by applying the Revised Universal Soil Loss Equation based on a GIS platform and multi-source input data to investigate associated drivers. The results established that soil erosion in both regions was substantially reduced by ecological restoration projects and significant land use/cover conversions. Landscape and geomorphological variables were found to be the dominant factors controlling soil erosion in the LP and KP, as they influenced land use patches and geomorphological patterns, respectively. The correlations between fragmentation metric indices and soil erosion indicated that the appropriately intensive fragmentation in the LP could mitigate or prevent soil erosion by disturbing its formation and transportation and ultimately positively influenced soil erosion control. Geomorphological patterns were also determinative factors, particularly for the KP, where almost all geomorphological variables were significantly correlated with the erosion modulus. Owing to the peculiar landform and landscape conditions in karst areas and loess hilly-gully areas, geomorphological and landscape variables should be considered when determining the main factors affecting soil erosion processes and integrated into the forecasting model to improve the accuracy of the simulation. The findings of this study are expected to (i) improve the efficacy of soil erosion control and (ii) promote the sustainable planning and management of land and soil resources.
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http://dx.doi.org/10.1007/s11356-020-11703-1DOI Listing
March 2021

Comprehensive utilization of foundry dust: Coal powder and clay minerals separation by ultrasonic-assisted flotation.

J Hazard Mater 2021 Jan 30;402:124124. Epub 2020 Sep 30.

State Key Laboratory of Materials Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address:

Clay sand casting generates a large amount of foundry dust (FD), and the presence of coal powder in the FD makes it difficult to recycle and utilize. The landfill of the FD creates a serious environmental pollution and wastes a valuable resource. To improve the above situation, the FD was analyzed and characterized by X-ray fluorescence spectrometer (XRF), X-ray diffraction (XRD) and electron probe microanalyzer (EPMA). An ultrasonic-assisted flotation process was developed for the comprehensive utilization of the FD, and the effects of ultrasonic time on the flotation performance and flotation kinetics were investigated. In addition, the two-stage flotation of the FD was conducted. Obtained results showed that the FD mainly consisted of coal powder and clay minerals, and the coal powder was covered by clay minerals. The separation efficiency of the coal powder and clay minerals can be significantly enhanced by ultrasonic pretreatment, and the optimal ultrasonic time was 30 min. The flotation kinetics analysis results indicated that the first-order model with rectangular distribution was more reasonable for the data fitting of the ultrasonic-assisted flotation. Furthermore, the concentrate and tailings obtained by the two-stage flotation had achieved an acceptable result, favoring the comprehensive utilization of the FD.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124124DOI Listing
January 2021

Bi metal/oxygen-deficient BiO with tetrahedral morphology and high photocatalytic activity.

Nanotechnology 2021 Feb;32(6):065702

Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi, 435002, People's Republic of China.

Vacancy-rich materials with high photocatalytic activity are of great interest for pollutants removal and play a significant role in green chemistry. Herein, we successfully synthesized Bi/BiO composite through hydrothermal route. In this case, the surface plasmon resonance effect of Bi and oxygen vacancies of BiO collectively increase the removal rate of pollutants. More importantly, the Bi/BiO composites have enhanced activity in the degradation of RhB, MO, BPA and CIP, and the reduction of Cr(VI) and PNA. Besides, an enhanced photocatalytic activity is due to the main reactive species of ·[Formula: see text] and h that is confirmed by trapping experiments and ESR analyses. The electronic structure and visible light harvesting of photocatalysts were measured and also theoretically calculated by using density functional theory and finite difference time domain calculations, DRS, VB x-ray photoelectron spectroscopy and Mott-Schottky plots, which allowed to propose a possible photocatalytic mechanism for the degradation process.
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http://dx.doi.org/10.1088/1361-6528/abc039DOI Listing
February 2021

Ecological restoration is not sufficient for reconciling the trade-off between soil retention and water yield: A contrasting study from catchment governance perspective.

Sci Total Environ 2021 Feb 2;754:142139. Epub 2020 Sep 2.

Guangdong Open Laboratory of Geospatial Information Technology and Application, Guangzhou Institute of Geography, Guangzhou 510070, PR China; Key Lab of Guangdong for Utilization of Remote Sensing and Geographical Information System, Guangzhou 510070, PR China. Electronic address:

Ecological restoration program (ERP) is widely recognized as an effective measure to combat land degradation and improve environmental quality. However, inappropriate ERPs lead to trade-offs between soil retention and water yield as well as conflicts of soil and water resources between the midstream and the downstream of catchment. This study aims to assess the efficiency of ERPs in soil erosion control and identify the trade-offs between soil retention and water yield through the lens of runoff and sediment regimes in contrasting catchments of the Loess Plateau (LP) and the Karst Plateau (KP). Although favorable climate and rapid vegetation restoration substantially reduced water erosion in both these areas, the hydrological responses were not the same because of climate differences. In the arid LP, water and energy variables correlated closely with vegetation cover. Excessive afforestation programs in drylands increased vegetation transpiration and soil evaporation, further exhausting soil water resources, and eventually causing water yield reduction. However, soil and water conservation programs (SWCPs) in the humid KP reduced sediment yield substantially, and the runoff remained stable. Significant runoff reduction in the midstream of the Yellow River aggravated water scarcity and threatened the downstream water demand. Meanwhile, sediment load decline in the LP and the KP impacted sediment deposition in the downstream and estuary formation. From the perspective of integrated catchment governance, human interventions including ERP and SWCP should be more sustainable and consider not only the target process at the local scale (intracoupling effect), but also unprecedented non-target process at the regional scale (telecoupling effect). In addition, it should allow for the supply-demand balance of competing soil and water resources to achieve the coordinated development of resources, environment, and production.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142139DOI Listing
February 2021

Coking-Resistant Iron Catalyst in Ethane Dehydrogenation Achieved through Siliceous Zeolite Modulation.

J Am Chem Soc 2020 09 11;142(38):16429-16436. Epub 2020 Sep 11.

Key Lab of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.

Nonoxidative dehydrogenation is promising for production of light olefins from shale gas, but current technology relies on precious Pt or toxic Cr catalysts and suffers from thermodynamically oriented coke formation. To solve these issues, the earth-abundant iron catalyst is employed, where Fe species are effectively modulated by siliceous zeolite, which is realized by the synthesis of Fe-containing MFI siliceous zeolite in the presence of ethylenediaminetetraacetic sodium (FeS-1-EDTA). Catalytic tests in ethane dehydrogenation show that this catalyst has a superior coke resistance in a 200 h run without any deactivation with extremely high activity and selectivity (e.g., 26.3% conversion and over 97.5% selectivity to ethene in at 873 K, close to the thermodynamic equilibrium limitation). Multiple characterizations demonstrate that the catalyst has uniformly and stably isolated Fe sites, which improves ethane dehydrogenation to facilitate the fast desorption of hydrogen and olefin products in the zeolite micropores and hinders the coke formation, as also identified by density functional calculations.
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http://dx.doi.org/10.1021/jacs.0c07792DOI Listing
September 2020

Unfolding the effectiveness of ecological restoration programs in combating land degradation: Achievements, causes, and implications.

Sci Total Environ 2020 Dec 10;748:141552. Epub 2020 Aug 10.

Guangdong Open Laboratory of Geospatial Information Technology and Application, Guangzhou Institute of Geography, Guangzhou 510070, PR China; Key Lab of Guangdong for Utilization of Remote Sensing and Geographical Information System, Guangzhou 510070, PR China.

Land degradation is one of the most serious environmental problems worldwide. To combat land degradation, China has implemented a series of ecological restoration programs (ERPs). This study selected the northern dryland of China as a case study to examine the efficiency of ERPs, and the response of soil loss to afforestation efforts and climatic conditions was discussed using the principles from the ecological theory of non-linear ecosystem dynamics. Owing to the combined impacts of declining wind speed and rapid vegetation restoration, the soil erosion for the entire region was substantially reduced from 1990 to 2015. However, the rainfall fluctuated considerably, particularly for the period from the late 1990s to early 2000s. Several drought events to some extent inhibited vegetation growth and further offset afforestation efforts, resulting in degradations in vegetation structure and soil retention function, which have been aggravating soil erosion since 2005. In certain representative sandstorm areas, limited increase in rainfall was not enough to promote vegetation growth, and therefore the vegetation cover did not present increasing trends and, in some cases, even declined significantly. The responses in terms of land degradation to climatic conditions and afforestation efforts behaved in a non-linear dynamic manner, providing essential insights into appropriate timings, climate-induced windows of opportunity, and risk in recovering and sustaining ecosystems, and eventually moving towards the land degradation neutrality (LDN) target. The climate-induced windows of opportunity and risk are critical in identifying the time for starting human interventions to mitigate and halt land degradation. Meanwhile, effective investment actions should be taken according to existing environmental conditions and critical thresholds, to achieve LDN at minimum risk and cost.
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http://dx.doi.org/10.1016/j.scitotenv.2020.141552DOI Listing
December 2020

In silico analysis of proteins and microRNAs related to human African trypanosomiasis in tsetse fly.

Comput Biol Chem 2020 Oct 24;88:107347. Epub 2020 Jul 24.

School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong; Hong Kong Bioinformatics Centre, The Chinese University of Hong Kong, Hong Kong.

Human African trypanosomiasis (HAT), also known as sleeping sickness, causes millions of deaths worldwide. HAT is primarily transmitted by the vector tsetse fly (Glossina morsitans). Early diagnosis remains a key objective for treating this disease. MicroRNAs (miRNAs) are evolutionarily conserved small non-coding RNAs that play key roles in vector-borne diseases. To date, the roles of proteins and miRNAs in HAT disease have not been thoroughly elucidated. In this study, we have re-annotated the function of protein-coding genes and identified several miRNAs based on a series of bioinformatics tools. A batch of 81.1 % of tsetse fly proteins could be determined homology in mosquito genome, suggesting their probable similar mechanisms in vector-borne diseases. A set of 11 novel salivary proteins and 14 midgut proteins were observed in the tsetse fly, which could be applied to the development of vaccine candidates for the control of HAT disease. In addition, 35 novel miRNAs were identified, among which 10 miRNAs were found to be unique in tsetse fly. Pathway analysis of these 10 miRNAs indicated that targets of miR-15a-5p were significantly enriched in the HAT-related neurotrophin signaling pathway. Besides, topological analysis of the miRNA-gene network indicated that miR-619-5p and miR-2490-3p targeted several genes that respond to trypanosome infection, including thioester-containing protein Tep1 and heat shock protein Hsp60a. In conclusion, our work helps to elucidate the function of miRNAs in tsetse fly and establishes a foundation for further investigations into the molecular regulatory mechanisms of HAT disease.
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http://dx.doi.org/10.1016/j.compbiolchem.2020.107347DOI Listing
October 2020

Network-based identification of biomarkers for colon adenocarcinoma.

BMC Cancer 2020 Jul 17;20(1):668. Epub 2020 Jul 17.

School of Mathematics and Statistics, Shandong University, Weihai, 264209, China.

Background: As one of the most common cancers with high mortality in the world, we are still facing a huge challenge in the prevention and treatment of colon cancer. With the rapid development of high throughput technologies, new biomarkers identification for colon cancer has been confronted with the new opportunities and challenges.

Methods: We firstly constructed functional networks for each sample of colon adenocarcinoma (COAD) by using a sample-specific network (SSN) method which can construct individual-specific networks based on gene expression profiles of a single sample. The functional genes and interactions were identified from the functional networks, respectively.

Results: Classification and subtyping were used to test the function of the functional genes and interactions. The results of classification showed that the functional genes could be used as diagnostic biomarkers. The subtypes displayed different mechanisms, which were shown by the functional and pathway enrichment analysis for the representative genes of each subtype. Besides, subtype-specific molecular patterns were also detected, such as subtype-specific clinical and mutation features. Finally, 12 functional genes and 13 functional edges could serve as prognosis biomarkers since they were associated with the survival rate of COAD.

Conclusions: In conclusion, the functional genes and interactions in the constructed functional network could be used as new biomarkers for COAD.
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http://dx.doi.org/10.1186/s12885-020-07157-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367377PMC
July 2020

Betulin alleviates on myocardial inflammation in diabetes mice via regulating Siti1/NLRP3/NF-κB pathway.

Int Immunopharmacol 2020 Aug 9;85:106653. Epub 2020 Jun 9.

Department of Cardiovascular Surgery, Fuwai Central China Cardiovascular Hospital, China; Department of Cardiovascular Surgery, Henan Provincial People's Hospital, China.

The aim of this study is to study the effect of betulin (BE) on myocardial injury in diabetic mice. Insulin-related indexes and inflammation-related cytokines are detected by commercial kits. The mechanism of BE on diabetic myocardial injury was studied by modern molecular biology techniques. BE significantly improved glocose tolerance, reduced lipid accumulation and reduced the content of inflammatory cytokines in diabetic mice. Furthermore, BE regulated Siti1/NLRP3/NF-κB signaling pathway in db/db mice and H9C2 cells. Siti1 inhibitor (EX-57) counteracted those changes. BE significantly protected against diabetic cardiomyopathy, which was related to the regulation of Siti1/NLRP3/NF-κB pathway.
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http://dx.doi.org/10.1016/j.intimp.2020.106653DOI Listing
August 2020

Genotype-Guided Dosing of Warfarin in Chinese Adults: A Multicenter Randomized Clinical Trial.

Circ Genom Precis Med 2020 08 8;13(4):e002602. Epub 2020 Jun 8.

Department of Cardiology, First People's Hospital of Xiangtan City, China (F.X.).

Background: Warfarin is an effective treatment for thromboembolic disease but has a narrow therapeutic index; optimal anticoagulation dosage can differ tremendously among individuals. We aimed to evaluate whether genotype-guided warfarin dosing is superior to routine clinical dosing for the outcomes of interest in Chinese patients.

Methods: We conducted a multicenter, randomized, single-blind, parallel-controlled trial from September 2014 to April 2017 in 15 hospitals in China. Eligible patients were ≥18 years of age, with atrial fibrillation or deep vein thrombosis without previous treatment of warfarin or a bleeding disorder. Nine follow-up visits were performed during the 12-week study period. The primary outcome measure was the percentage of time in the therapeutic range of the international normalized ratio during the first 12 weeks after starting warfarin therapy.

Results: A total of 660 participants were enrolled and randomly assigned to a genotype-guided dosing group or a control group under standard dosing. The genotype-guided dosing group had a significantly higher percentage of time in the therapeutic range than the control group (58.8% versus 53.2% [95% CI of group difference, 1.1-10.2]; =0.01). The genotype-guided dosing group also achieved the target international normalized ratio sooner than the control group. In subgroup analyses, warfarin normal sensitivity group had an even higher percentage of time in the therapeutic range during the first 12 weeks compared with the control group (60.8% versus 48.9% [95% CI, 1.1-24.4]). The incidence of adverse events was low in both groups.

Conclusions: The outcomes of genotype-guided warfarin dosing were superior to those of clinical standard dosing. These findings raise the prospect of precision warfarin treatment in China. Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT02211326.
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http://dx.doi.org/10.1161/CIRCGEN.119.002602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439928PMC
August 2020

Flower-like BiSiO/BiMoO heterostructures for enhanced photocatalytic degradation of ciprofloxacin.

Nanotechnology 2020 Aug 7;31(34):345604. Epub 2020 May 7.

College of Chemistry and Chemical Engineering, Hubei Key Laboratory of Pollutant Analysis and Reuse Technology, Hubei Normal University, Huangshi, Hubei 435002, People's Republic of China.

BiSiO/BiMoO photocatalysts with heterostructures were successfully prepared using a one-pot solvothermal route. The effect of the molybdenum source on composite formation is discussed. Under ultraviolet light irradiation, the BiSiO/BiMoO heterojunction photocatalyst exhibited higher photocatalytic performance than BiSiO and BiMoO towards the degradation of ciprofloxacin (CIP). This dramatically enhanced photoactivity can be ascribed to the construction of a heterojunction interface between BiSiO and BiMoO, which not only suppresses the recombination of photoexcited charge carriers but also enhances light absorption. In addition, from a practical point of view, the the effect of initial CIP concentration and coexisting ions on the photodegradation process using as-prepared BiSiO/BiMoO heterojunction photocatalysts was explored. Trapping experiments demonstrate that photoexcited holes and superoxide radicals are the main active species in the photodegradation of CIP over BiSiO/BiMoO heterojunctions. Meanwhile, the conduction band and valence band potentials of BiSiO and BiMoO were measured by density functional theory calculation, diffuse reflectance spectroscopy and Mott-Schottky curves. A possible photocatalytic mechanism for CIP degradation over the BiSiO/BiMoO heterojunction is proposed.
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http://dx.doi.org/10.1088/1361-6528/ab912fDOI Listing
August 2020

Immunogenicity and protective efficacy of an EB66 cell culture-derived duck Tembusu virus vaccine.

Avian Pathol 2020 Oct 2;49(5):448-456. Epub 2020 Jul 2.

Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.

The avian EB66 cell line, derived from duck embryonic stem cells, has been widely used for producing human and animal therapeutic proteins and vaccines. In current study we evaluated the potential use of EB66 cell line in a cell culture-derived duck Tembusu virus (DTMUV) vaccine development. After optimizing the growth conditions of DTMUV HB strain in EB66 cells, we successfully generated three batches of viruses with ELD titres of 10/0.1 ml, 10/0.1 ml and 10/0.1 ml, respectively, for using in the preparation of inactivated vaccines. The immunogenicity and protective efficacy of these EB66 cells-derived inactivated vaccines were examined in ducks. Results indicated that all three batches of vaccines induced haemagglutination-inhibition (HI) antibody response in immunized birds at 2 weeks after a single immunization. Immunized ducks and ducklings were protected against a virulent challenge at 4 weeks after a booster immunization. The duration of immunity was for 3-4 months after a booster immunization. These results demonstrated the feasibility of using EB66 cell line to grow up DTMUV for vaccine preparation. Duck Tembusu virus can be propagated in EB66 cells. EB66 cell-derived inactivated DTMUV vaccines are immunogenic and can provide protection against a virulent challenge. A long-lasting immunity is induced after a booster immunization.
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http://dx.doi.org/10.1080/03079457.2020.1763914DOI Listing
October 2020

A hot-blast warming facility for simulating global warming in low-stature crop systems and its application case to assess elevated temperature effects on rice in Central China.

Plant Methods 2020 23;16:57. Epub 2020 Apr 23.

1National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070 Hubei China.

Background: To study the impact of climate warming on crops, it is crucial to have a warming equipment suitable for their field environment. A facility is needed that can provide suitable combinations of different temperatures at reasonable cost for large plots.

Results: Here, an additional field warming facility option named the hot-blast warming facility (HBWF), which comprised heaters, blowers, wind breaks, and a control board was developed. An application case based on HBWF was carried out to assess elevated temperature effects on rice in Central China during 2015 and 2016. We tested four elevated temperature treatments on four rice cultivars under paddy field conditions and measured yield and its components. Heating convection air directly, the facility could increase the temperature of the rice canopy up to 1-2 °C, which could properly simulate global warming. Considering the costs, the HBWF reduced the operating costs because of its relatively lower power consumption (0.164 kW/m), which was 80% lower than that of Free Air Temperature Increase. Our results demonstrate that the HBWF could build a 25 m homogeneous heating area and had little effect on the relative humidity under a paddy field environment. Warming treatments significantly reduced the grain yield by 4.4-22.7% in 2015, and 30.8-61.9% in 2016, compared to the control. The main contribution to the significant decrease of the grain yields was the decrease in seed setting rate. Moreover, a reduction of 1000-grain weight led to the decline in grain yield. The increasing ranges of the temperature simulated by HBWF were stable in different years, however, whether the elevated treatments demonstrated significant difference on rice growth mainly decided by the basic atmospheric temperature (as the control) during the growth period.

Conclusions: The new warming facility is suitable for field trials to assess elevated temperature combinations and provides an extra equipment option for use in elevated temperature research in the future.
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http://dx.doi.org/10.1186/s13007-020-00598-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181580PMC
April 2020

Subtractive proteomics and systems biology analysis revealed novel drug targets in Mycoplasma genitalium strain G37.

Microb Pathog 2020 Aug 25;145:104231. Epub 2020 Apr 25.

School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong SAR, PR China.

Mycoplasma genitalium is one of the sexually transmitted pathogens that cause significant morbidity in the host. The development of effective therapeutic procedures is urgently needed to counter the multi-drug resistant events imposed by this pathogen. In the current version of M. genitalium G37 genome, 512 open reading frames have been identified. The function of 91 proteins encoded by M. genitalium genes was found to be hypothetical and these proteins were termed hypothetical proteins (HPs). This study aims to carry out functional characterization of HPs by a systems biology approach. Functional assignments of 61 HPs were made with high confidence. They belong to different functional groups, such as DNA-binding proteins, helicases and transporters. Approximately 26% of HPs were identified as transporters, suggesting that M. genitalium is likely to rely on the exogenous nutrient supply for survival. A group of 20 proteins was predicted to be virulence factors, indicating the pathogenic characteristics of M. genitalium. Among the coding proteins, six proteins were pathogen-specific and could serve as potential drug targets by subtractive proteomics analysis. Network analysis of the HPs suggested that several critical proteins were involved in SOS response and stringent response in M. genitalium. These findings provided a better picture of M. genitalium genome and novel clues for studying the potential infection mechanism in this bacterium.
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http://dx.doi.org/10.1016/j.micpath.2020.104231DOI Listing
August 2020

Identification, functional annotation, and stability analysis of miRNA in Dermatophagoides pteronyssinus.

Allergy 2020 05 5;75(5):1237-1240. Epub 2019 Dec 5.

School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong.

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http://dx.doi.org/10.1111/all.14123DOI Listing
May 2020

Improved annotation of genome using bioinformatics analysis.

PeerJ 2019 9;7:e7862. Epub 2019 Oct 9.

College of Chemical Engineering, Huaqiao University, Xiamen, PR China.

, a sand fly, is a vector-spreading pathogenic protozoan in the New World. MicroRNA (miRNA) is evolutionarily-conserved non-coding RNA, which plays critical roles in various biological processes. To date, the functions of most proteins in are unknown, and few studies have addressed the roles of miRNAs in this species. In the present study, we re-annotated the protein-coding genes and identified several miRNAs using a set of comparative genomics tools. A large number of proteins were found to be homologous with those in the mosquito genome, indicating that they may have experienced similar selective pressures. Among these proteins, a set of 19 putative salivary proteins were identified, which could be used for studying the transmission of Leishmania. Twenty-one novel miRNAs were characterized, including two miRNAs, miR-4113-5p and miR-5101, which are unique to . Many of the targets of these two genes were found to be involved in ATP hydrolysis-coupled proton transport, suggesting that they may have important roles in the physiology of energy production. Topology analysis of the miRNA-gene network indicated that miR-9388-5p and miR-3871-5p regulate several critical genes in response to disease development. In conclusion, our work provides a basis for improving the genome annotation of , and opens a new door to understanding the molecular regulatory mechanisms in this species.
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http://dx.doi.org/10.7717/peerj.7862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6790103PMC
October 2019

Antioxidant activity of Momordica charantia polysaccharide and its derivatives.

Int J Biol Macromol 2019 Oct 22;138:673-680. Epub 2019 Jul 22.

Active Carbohydrate Research Institute, Chongqing Key Laboratory of Inorganic Functional Materials, College of Chemistry, Chongqing Normal University, Chongqing 401331, China.

Momordica charantia polysaccharide (MCP) was extracted by hot water and chemically modified to obtain phosphorylated Momordica charantia polysaccharide (P-MCP) with degree of substitution 0.12 and sulfated Momordica charantia polysaccharide (S-MCP) with degree of substitution 0.45. The sugar content of the three polysaccharides was determined by phenol sulfuric acid method, 74.0%, 68.1% and 59.8% respectively. The scavenging ability of three polysaccharides to superoxide anion, hydroxyl radical and DPPH radical, as well as their anti-lipid peroxidation and reduction ability were determined. The results showed that the antioxidant activity of polysaccharides varied with different chemical modifications.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.07.129DOI Listing
October 2019

Development of a Hemagglutination Inhibition Assay for Duck Tembusu Virus.

Avian Dis 2019 06;63(2):298-301

Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China,

The HB strain of duck Tembusu virus (DTMUV) propagated in the brains of newborn mice was used to prepare antigens for use in the hemagglutination inhibition (HI) test. Results showed that such prepared antigens are highly specific to the serum samples derived from DTMUV-infected animals. No spurious hemagglutination reactions against serum samples specific to avian influenza virus H5, H7, H9 subtypes, Newcastle disease virus, egg drop syndrome virus, duck plague virus, and duck hepatitis A virus were observed. The HI test can detect specific antibodies in the serum samples as early as day 4 after experimental infection of ducks with DTMUV. When compared to a virus neutralization test, the sensitivity is 100%. Overall, the HI test developed is highly specific to DTMUV and can be used in clinical diagnosis of diseases and in vaccine studies to monitor the kinetics of antibody response.
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http://dx.doi.org/10.1637/11954-082018-Reg.1DOI Listing
June 2019

Psychometric Properties and Factor Structure of the Chinese Version of the Hospital Anxiety and Depression Scale in People Living With HIV.

Front Psychiatry 2019 16;10:346. Epub 2019 May 16.

Center for Infectious Diseases, Beijing You'an Hospital, Capital Medical University, Beijing, China.

The population of people living with HIV (PLWH) is growing in number and usually results in mental health problems that impact their quality of life. Therefore, valid instruments and screening methods for psychological disorders are of great significance. The Hospital Anxiety and Depression Scale (HADS) reveals good psychometric properties, but shows ambiguous results in factor structure. This study aims to evaluate psychometric properties in terms of the internal reliability and structure validity of the Chinese version of the HADS (C-HADS) in a large sample of PLWH in China. The C-HADS was administered to 4,102 HIV-infected adults at an HIV clinic in China. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were performed to examine the factor structure. Measurement invariance was assessed across gender and course of infection. Internal reliability was also assessed. A bifactor model with anomalous loadings of items 7, 8, and 10 fits the data best and holds measurement invariance across gender and course of infection. Internal reliability was good with all Cronbach's alphas > 0.70 and Spearman's ρ between 0.30 and 0.70. The C-HADS has good psychometric properties in terms of internal reliability and structure validity of a bifactor model. The C-HADS is recommended to be used as a total scale that measures general psychological distress, instead of anxiety and depression separately, when applied to PLWH. Further studies are needed to evaluate criterion validity, the cutoff score, and the effect of wording and scoring of the HADS.
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http://dx.doi.org/10.3389/fpsyt.2019.00346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6531499PMC
May 2019

Depleting T regulatory cells by targeting intracellular Foxp3 with a TCR mimic antibody.

Oncoimmunology 2019;8(7):1570778. Epub 2019 Apr 15.

Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.

Depletion of T regulatory cells (Tregs) in the tumor microenvironment is a promising cancer immunotherapy strategy. Current approaches for depleting Tregs are limited by lack of specificity and concurrent depletion of anti-tumor effector T cells. The transcription factor forkhead box p3 (Foxp3) plays a central role in the development and function of Tregs and is an ideal target in Tregs, but Foxp3 is an intracellular, undruggable protein to date. We have generated a T cell receptor mimic antibody, "Foxp3-#32," recognizing a Foxp3-derived epitope in the context of HLA-A*02:01. The mAb Foxp3-#32 selectively recognizes CD4 + CD25 + CD127 and Foxp3 + Tregs also expressing HLA-A*02:01 and depletes these cells via antibody-mediated cellular cytotoxicity. Foxp3-#32 mAb depleted Tregs in xenografts of PBMCs from a healthy donor and ascites fluid from a cancer patient. A TCRm mAb targeting intracellular Foxp3 epitope represents an approach to deplete Tregs.
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http://dx.doi.org/10.1080/2162402X.2019.1570778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527296PMC
April 2019

Investigating function roles of hypothetical proteins encoded by the Mycobacterium tuberculosis H37Rv genome.

BMC Genomics 2019 May 21;20(1):394. Epub 2019 May 21.

School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, N.T, Hong Kong SAR.

Background: Mycobacterium tuberculosis (MTB) is a common bacterium causing tuberculosis and remains a major pathogen for mortality. Although the MTB genome has been extensively explored for two decades, the functions of 27% (1051/3906) of encoded proteins have yet to be determined and these proteins are annotated as hypothetical proteins.

Methods: We assigned functions to these hypothetical proteins using SSEalign, a newly designed algorithm utilizing structural information. A set of rigorous criteria was applied to these annotations in order to examine whether they were supported by each parameter. Virulence factors and potential drug targets were also screened among the annotated proteins.

Results: For 78% (823/1051) of the hypothetical proteins, we could identify homologs in Escherichia coli and Salmonella typhimurium by using SSEalign. Functional classification analysis indicated that 62.2% (512/823) of these annotated proteins were enzymes with catalytic activities and most of these annotations were supported by at least two other independent parameters. A relatively high proportion of transporter was identified in MTB genome, indicating the potential frequent transportation of frequent absorbing essential metabolites and excreting toxic materials in MTB. Twelve virulence factors and ten vaccine candidates were identified within these MTB hypothetical proteins, including two genes (rpoS and pspA) related to stress response to the host immune system. Furthermore, we have identified six novel drug target candidates among our annotated proteins, including Rv0817 and Rv2927c, which could be used for treating MTB infection.

Conclusions: Our annotation of the MTB hypothetical proteins will probably serve as a useful dataset for future MTB studies.
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http://dx.doi.org/10.1186/s12864-019-5746-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6528289PMC
May 2019

Product Selectivity Controlled by Nanoporous Environments in Zeolite Crystals Enveloping Rhodium Nanoparticle Catalysts for CO Hydrogenation.

J Am Chem Soc 2019 May 14;141(21):8482-8488. Epub 2019 May 14.

Key Lab of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering , Zhejiang University , Hangzhou 310027 , China.

Supported rhodium nanoparticles (NPs) are well-known for catalyzing methanation in CO hydrogenation. Now we demonstrate that the selectivity in this process can be optimized for CO production by choice of molecular sieve crystals as supports. The NPs are enveloped within the crystals with controlled nanopore environments that allow tuning of the catalytic selectivity to minimize methanation and favor the reverse water-gas shift reaction. Pure silica MFI (S-1)-fixed rhodium NPs exhibited maximized CO selectivity at high CO conversions, whereas aluminosilicate MFI zeolite-supported rhodium NPs displayed high methane selectivity under the equivalent conditions. Strong correlations were observed between the nanoporous environment and catalytic selectivity, indicating that S-1 minimizes hydrogen spillover and favors fast desorption of CO to limit deep hydrogenation. Materials in this class appear to offer appealing opportunities for tailoring selective supported catalysts for a variety of reactions.
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http://dx.doi.org/10.1021/jacs.9b01555DOI Listing
May 2019

Gene Expression Classification of Lung Adenocarcinoma into Molecular Subtypes.

IEEE/ACM Trans Comput Biol Bioinform 2020 Jul-Aug;17(4):1187-1197. Epub 2019 Mar 18.

As one of the most common malignancies in the world, lung adenocarcinoma (LUAD) is currently difficult to cure. However, the advent of precision medicine provides an opportunity to improve the treatment of lung cancer. Subtyping lung cancer plays an important role in performing a specific treatment. Here, we developed a framework that combines k-means clustering, t-test, sensitivity analysis, self-organizing map (SOM) neural network, and hierarchical clustering methods to classify LUAD into four subtypes. We determined that 24 differentially expressed genes could be used as therapeutic targets, and five genes (i.e., RTKN2, ADAM6, SPINK1, COL3A1, and COL1A2) could be potential novel markers for LUAD. Multivariate analysis showed that the four subtypes could serve as prognostic subtypes. Representative genes of each subtype were also identified, which could be potentially targetable markers for the different subtypes. The function and pathway enrichment analyses of these representative genes showed that the four subtypes have different pathological mechanisms. Mutations associated with the subtypes, e.g., epidermal growth factor receptor (EGFR) mutations in subtype 4 and tumor protein p53 (TP53) mutations in subtypes 1 and 2, could serve as potential markers for drug development. The four subtypes provide a foundation for subtype-specific therapy of LUAD.
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http://dx.doi.org/10.1109/TCBB.2019.2905553DOI Listing
April 2021

Development and validation of a competitive ELISA based on bacterium-original virus-like particles of serotype O foot-and-mouth disease virus for detecting serum antibodies.

Appl Microbiol Biotechnol 2019 Apr 19;103(7):3015-3024. Epub 2019 Feb 19.

State Key Laboratory of Veterinary Etiological Biology, OIE/China national Foot-and-mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, 730046, Gansu, China.

Foot-and-mouth disease (FMD) is a highly contagious disease that affects all susceptible cloven-hoofed animals, resulting in considerable economic losses to animal industries worldwide. Numerous categories of enzyme-linked immunosorbent assays (ELISA) have been developed and widely used to evaluate herd immunity. Manufacturing inactivated FMD virus (FMDV) as a diagnostic antigen requires a facility with a high level of biosafety, but this requirement raises concern on viral leakage. In our previous study, bacterium-original FMD virus-like particles (VLPs) resemble the authentic FMDV and induce protective immunity against homologous viral challenges, thereby demonstrating that they are sufficiently safe without limitations on biosafety facilities and easily prepared. Herein, we developed a competitive ELISA (cELISA) based on FMDV-VLPs as a diagnostic antigen to evaluate herd immunity. The criterion of this cELISA was determined by detecting panels of positive sera with different antibody titers and negative sera. The working parameter of cELISA was optimized, and samples with a percentage inhibition of ≥ 50% were considered positive. The specificity of cELISA to test 277 serum samples with various antibody titers was 100%, and the sensitivity reached 96%. The coincidence rates of cELISA with a VDPro® FMDV and a PrioCHECK® FMDV type O antibody ELISA kit were 97.8% and 98.2%, respectively. Repeatability tests demonstrated that the coefficients of variation within and between runs were less than 7% and 14%, respectively. Our data demonstrated that cELISA based on bacterium-original VLPs had high specificity, sensitivity, and reproducibility. The cELISA could also be used for evaluating vaccination herd immunity effects, especially in developing countries.
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http://dx.doi.org/10.1007/s00253-019-09680-8DOI Listing
April 2019