Publications by authors named "Zhiyong Zhang"

904 Publications

The Resting State Central Auditory Network: a Potential Marker of HIV-Related Central Nervous System Alterations.

Ear Hear 2022 Jan 17. Epub 2022 Jan 17.

Shanghai Public Health Clinical Center, Fudan University, Shanghai, China Shanghai Key Laboratory of Magnetic Resonance, East China Normal University, Shanghai, China Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, USA Creare, LLC, Hanover, New Hampshire, USA University of British Columbia, Vancouver, Canada These authors contributed equally to this work.

Objective: HIV positive (HIV+) individuals with otherwise normal hearing ability show central auditory processing deficits as evidenced by worse performance in speech-in-noise perception compared with HIV negative (HIV-) controls. HIV infection and treatment are also associated with lower neurocognitive screening test scores, suggesting underlying central nervous system damage. To determine how central auditory processing deficits in HIV+ individuals relate to brain alterations in the cortex involved with auditory processing, we compared auditory network (AN) functional connectivity between HIV+ adults with or without speech-in-noise perception difficulties and age-matched HIV- controls using resting-state fMRI.

Design: Based on the speech recognition threshold of the hearing-in-noise test, twenty-seven HIV+ individuals were divided into a group with speech-in-noise perception abnormalities (HIV+SPabnl, 38.2 ± 6.8 years; 11 males and 2 females) and one without (HIV+SPnl 34.4 ± 8.8 years; 14 males). An HIV- group with normal speech-in-noise perception (HIV-, 31.3 ± 5.2 years; 9 males and 3 females) was also enrolled. All of these younger and middle-aged adults had normal peripheral hearing determined by audiometry. Participants were studied using resting-state fMRI. Independent component analysis was applied to identify the AN. Group differences in the AN were identified using statistical parametric mapping.

Results: Both HIV+ groups had increased functional connectivity (FC) in parts of the AN including the superior temporal gyrus, middle temporal gyrus, supramarginal gyrus, and Rolandic operculum compared to the HIV- group. Compared with the HIV+SPnl group, the HIV+SPabnl group showed greater FC in parts of the AN including the middle frontal and inferior frontal gyri.

Conclusions: The classical auditory areas in the temporal lobe are affected by HIV regardless of speech perception ability. Increased temporal FC in HIV+ individuals might reflect functional compensation to achieve normal primary auditory perception. Furthermore, increased frontal FC in the HIV+SPabnl group compared with the HIV+SPnl group suggest that speech-in-noise perception difficulties in HIV-infected adults also affect areas involved in higher-level cognition, providing imaging evidence consistent with the hypothesis that HIV-related neurocognitive deficits can include central auditory processing deficits.
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http://dx.doi.org/10.1097/AUD.0000000000001186DOI Listing
January 2022

More opportunities more species: Pleistocene differentiation and northward expansion of an evergreen broad-leaved tree species Machilus thunbergii (Lauraceae) in Southeast China.

BMC Plant Biol 2022 Jan 17;22(1):35. Epub 2022 Jan 17.

Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi, China.

Background: The broad continuum between tropical and temperate floras in Eastern Asia (EAS) are thought to be one of the main factors responsible for a prominent species diversity anomaly of temperate plants between EAS and eastern North America (ENS). However, how the broad continuum and niche evolution between tropical and temperate floras in EAS contributes to lineage divergence and species diversity remains largely unknown.

Results: Population genetic structure, demography, and determinants of genetic structure [i.e., isolation-by-distance (IBD), isolation-by-resistance (IBR), and isolation-by-environment (IBE)] of Machilus thunbergii Sieb. et Zucc. (Lauraceae) were evaluated by examining sequence variation of ten low-copy nuclear genes across 43 populations in southeast China. Climatic niche difference and potential distributions across four periods (Current, mid-Holocene, the last glacial maximum, the last interglacial) of two genetic clusters were determined by niche modelling. North and south clusters of populations in M. thunbergii were revealed and their demarcation line corresponds well with the northern boundary of tropical zone in China of Zhu & Wan. The divergence time between the clusters and demographic expansion of M. thunbergii occurred after the mid-Pleistocene climate transition (MPT, 0.8-1.2 Ma). Migration rates between clusters were asymmetrical, being much greater from north to south than the reverse. Significant effects of IBE, but non-significant effects of IBD and IBR on population genetic divergence were detected. The two clusters have different ecological niches and require different temperature regimes.

Conclusions: The north-south genetic differentiation may be common across the temperate-tropical boundary in southeast China. Divergent selection under different temperature regimes (possibly above and below freezing temperature in winter) could account for this divergence pattern. The broad continuum between tropical and temperate floras in EAS may have provided ample opportunities for tropical plant lineages to acquire freezing tolerance and to colonize the temperate regions during the late-Cenozoic global cooling. Our findings shed deeper insights into the high temperate plant species diversity in EAS.
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http://dx.doi.org/10.1186/s12870-021-03420-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762935PMC
January 2022

Detection of traumatic internal carotid artery pseudoaneurysm by postmortem imaging: A case report.

Medicine (Baltimore) 2022 Jan;101(2):e28544

Department of Radiology, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Rationale: Postmortem imaging (PMI), including computed tomography (PMCT), postmortem computed tomography angiography (PMCTA), and postmortem magnetic resonance imaging (PMMRI), is rapidly becoming effective and a practical method in forensic medicine. This study aimed to present a specific forensic case in which the PMI approach and its applications were used.

Patient Concerns: A 40-year-old male patient had moderate unilateral nose bleeding constantly 10 times after suffering from a head injury induced by a car accident. After a bilateral massive nose bleeding for the last time, he died from hemorrhagic shock. Traumatic internal carotid artery pseudoaneurysm (TICAP) was suspected in this patient.

Diagnosis, Interventions, And Outcomes: A whole-body scanning was performed using PMCT and PMMRI. Then, PMCTA using left ventricular cardiac puncture was also implemented. A water-soluble contrast agent was injected into the left ventricle and pumped toward the intracranial, followed by a repeated whole-body PMCT scan. The PMCT/PMMRI detected a high-density/signal mass inside the left sphenoid sinus. The PMCTA detected a distinct leakage of the contrast agent into the left sphenoid sinus from an adjacent aneurysm of the C3 section of the left internal carotid artery. Autopsy and histology confirmed a TICAP inside the sphenoid sinus.

Lessons: This case showed that the PMI was of great value for identifying the cause of death in special cases. When vascular lesions are suspected in the body, PMI and especially the PMCTA approach may be an effective detection method.
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http://dx.doi.org/10.1097/MD.0000000000028544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758031PMC
January 2022

Clinical and genetic features of Chinese pediatric patients with severe congenital protein C deficiency who first presented with purpura fulminans: A case series study and literature review.

Thromb Res 2022 Jan 3;210:70-77. Epub 2022 Jan 3.

Department of Hematology and Oncology, Children's Hospital of Chongqing Medical University, Chongqing, China; National Clinical Research Center for Child Health and Disorders, Chongqing, China; Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Pediatrics, Chongqing, China. Electronic address:

Introduction: Purpura fulminans (PF) is a hematological emergency that can be caused by severe congenital protein C (PC) deficiency. It has been rarely reported in the Chinese population. We aimed to characterize the clinical and genetic features of Chinese pediatric patients with severe congenital PC deficiency who first presented with PF.

Materials And Methods: Twelve pediatric patients were diagnosed with severe congenital PC deficiency with PF, which was diagnosed based on our hospital records and previous reports from 1988 to July 2021 in China. We evaluated the clinical and genetic features of these patients.

Results: Nine patients (9/12, 75%) had onsets that were observed within the first 48 h after birth. Six patients had a family history of thromboembolism. There was no consanguinity. Other symptoms were intracranial thrombosis or hemorrhage (4, 33.3%), ocular lesions (2, 16.7%), gastrointestinal hemorrhage (2, 16.7%) and kidney infarction before birth (1, 8.3%). All but one of the patients (one case not detected) had a plasma PC activity of <10%. The genetic study indicated that in the eight patients with inherited PC deficiency, two were homozygous, five were compound heterozygous and one was heterozygous for PC deficiency.

Conclusion: This is the first and largest case series of Chinese pediatric patients with severe congenital PC deficiency who first presented with PF. It has been shown that treatment with both fresh frozen plasma and anticoagulants is recommended when PC concentrate is not easily available, especially in developing countries.
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http://dx.doi.org/10.1016/j.thromres.2021.12.026DOI Listing
January 2022

Integrative omics analysis reveals the protective role of vitamin C on perfluorooctanoic acid-induced hepatoxicity.

J Adv Res 2022 Jan 15;35:279-294. Epub 2021 Apr 15.

Laboratory of Environmental Pollution and Integrative Omics, Guilin Medical University, Guilin, PR China.

Introduction: Perfluorooctanoic acid (PFOA) is a compound used as an industrial surfactant in chemical processes worldwide. Population and cross-sectional studies have demonstrated positive correlations between PFOA levels and human health problems.

Objectives: Many studies have focused on the hepatotoxicity and liver problems caused by PFOA, with little attention to remediation of these problems. As an antioxidant, vitamin C is frequently utilized as a supplement for hepatic detoxification.

Methods: In this study, we use a mouse model to study the possible role of vitamin C in reducing PFOA-induced liver damage. Based on comparative transcriptomic and metabolomic analysis, we elucidate the mechanisms underlying the protective effect of vitamin C.

Results: Our results show that vitamin C supplementation reduces signs of PFOA-induced liver damage including total cholesterol and triglyceride levels increase, liver damage markers aspartate, transaminase, and alanine aminotransferase elevation, and liver enlargement. Further, we show that the protective role of vitamin C is associated with signaling networks control, suppressing linoleic acid metabolism, reducing thiodiglycolic acid, and elevating glutathione in the liver.

Conclusion: The findings in this study demonstrate, for the first time, the utility of vitamin C for preventing PFOA-induced hepatotoxicity.
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http://dx.doi.org/10.1016/j.jare.2021.04.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8721266PMC
January 2022

Fast magnetic resonance elastography with multiphase radial encoding and harmonic motion sparsity based reconstruction.

Phys Med Biol 2022 Jan 11. Epub 2022 Jan 11.

School of Biomedical Engineering, Shanghai Jiao Tong University, No.1954, Huashan Road, Xuhui District, Shanghai, 200030, CHINA.

Objective: To achieve fast magnetic resonance elastography (MRE) at a low frequency for better shear modulus estimation of the brain.

Approach: We proposed a multiphase radial DENSE MRE (MRD-MRE) sequence and an improved GRASP algorithm utilizing the sparsity of the harmonic motion (SH-GRASP) for fast MRE at 20 Hz. For the MRD-MRE sequence, the initial position encoded by one spatial modulation of magnetization (SPAMM) was decoded by an arbitrary number of readout blocks without increasing the number of phase offsets. Based on the harmonic motion, a modified total variation and temporal Fourier transform were introduced to utilize the sparsity in the temporal domain. Both phantom and brain experiments were carried out and compared with that from multiphase Cartesian DENSE-MRE (MCD-MRE), and conventional gradient echo sequence (GRE-MRE). Reconstruction performance was also compared with GRASP and compressed sensing.

Main Results: Results showed the scanning time of a fully sampled image with four phase offsets for MRD-MRE was only 1/5 of that from GRE-MRE. The wave patterns and estimated stiffness maps were similar to those from MCD-MRE and GRE-MRE. With SH-GRASP, the total scan time could be shortened by additional 4 folds, achieving a total acceleration factor of 20. Better metric values were also obtained using SH-GRASP for reconstruction compared with other algorithms.

Significance: The MRD-MRE sequence and SH-GRASP algorithm can be used either in combination or independently to accelerate MRE, showing the potentials for imaging the brain as well as other organs.
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http://dx.doi.org/10.1088/1361-6560/ac4a42DOI Listing
January 2022

Trp2 Peptide-Assembled Nanoparticles with Intrinsically Self-Chelating Cu Properties for PET Imaging Tracking and Dendritic Cell-Based Immunotherapy against Melanoma.

ACS Appl Bio Mater 2021 Jul 16;4(7):5707-5716. Epub 2021 Jun 16.

CAS Key Laboratory for the Biological Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.

Dendritic cell-based immunotherapy, in which the antigen is effectively delivered to dendritic cells and then the dendritic cells stimulated by the antigen migrate to draining lymph nodes (DLNs) to induce the CD8 T-cell immune response, shows great promise for tumor immunotherapy. In this study, we used coassembled nanoparticles formed by Trp2 antigen and the conjugates of short-chain poly(ethylene glycol) (PEG) and pyropheophorbide-A (PPa) (Trp2/PPa-PEGm) to deliver Trp2 to DCs. Intrinsically self-chelating Cu of coassemblies could be used to sensitively image the migration of DCs by positron emission tomography (PET) imaging. The coassemblies of the Trp2 antigen were efficiently engulfed by DCs without causing DC cytotoxicity and induced DC maturation. After injection of DCs labeled by coassemblies of the Trp2 antigen, the homing of DCs to DLNs could be sensitively observed by PET imaging. The C57BL/6 mice injected with DCs containing the Trp2/PPa-PEGm NP showed antigen-specific immune responses including enhanced interferon-γ (IFN-γ) production, splenocyte proliferation, and percentage of IFN-γ-secreting CD8 T cells. In addition, C57BL/6 mice inoculated with B16-F10 tumor cells showed delayed tumor growth after immunization with the Trp2/PPa-PEGm NP-labeled DC vaccine and enhanced infiltration of CD8 T cells in tumors.
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http://dx.doi.org/10.1021/acsabm.1c00480DOI Listing
July 2021

Associations Between Blood Pressure and Accelerated DNA Methylation Aging.

J Am Heart Assoc 2022 Jan 8:e022257. Epub 2022 Jan 8.

Department of Occupational Health and Environmental Health School of Public Health Guangxi Medical University Nanning Guangxi China.

Background Individuals of the same chronological age may exhibit diverse susceptibilities to death. However, few studies have investigated the associations between blood pressure and the accelerated aging. Methods and Results A cross-sectional study was conducted in 288 adults aged ≥50 years. We assessed the DNA methylation-based measures of biological age using CpG sites on the Illumina HumanMethylationEPIC BeadChip. Epigenetic age acceleration metrics were derived by regressing residuals (ΔAge) and ratios (aging rate) of DNA methylation age on chronological age. Dose-response relationships between blood pressure and epigenetic age acceleration were quantified using multiple linear regression and restricted cubic regression models. We found that each 10-mm Hg increase in systolic blood pressure was associated with 0.608 (95% CI, 0.231-0.984) years increase in ΔAge and 0.007 (95% CI, 0.002-0.012) increase in aging rate; meanwhile, for pulse pressure, the increase was 1.12 (95% CI, 0.625-1.61) years for ΔAge and 0.013 (95% CI, 0.007-0.020) for aging rate. Subgroup analysis showed that the significant associations of systolic blood pressure and pulse pressure with epigenetic age acceleration appeared to be limited to women, although interactions between blood pressure and sex were not significant ( values for interaction >0.05). The combination of women and hypertension was associated with a much higher increase in ΔAge (β [95% CI], 4.05 [1.07-7.02]) and aging rate (β [95% CI], 0.047 [0.008-0.087]), compared with male participants without hypertension. Conclusions Our findings suggested that high systolic blood pressure and pulse pressure were associated with the epigenetic age acceleration, providing important clues for relationships between blood pressure and epigenetic aging.
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http://dx.doi.org/10.1161/JAHA.121.022257DOI Listing
January 2022

Clinical characteristics and outcomes of chronic nonbacterial osteomyelitis in children: a multicenter case series.

Pediatr Rheumatol Online J 2022 Jan 3;20(1). Epub 2022 Jan 3.

Department of Allergy Immunology and Rheumatology, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, China.

Objective: The aim of this study was to evaluate demographic, clinical, laboratory, imaging, histopathology characteristics, and treatment responses of children with Chronic nonbacterial osteomyelitis (CNO).

Methods: Retrospective multi-center case series study of pediatric patients diagnosed with CNO treated at five tertiary centers in south China.

Results: Totally there were 18 patients diagnosed as CNO between 2014 and 2020. The median age of onset was 9.2 years (range 3.7-13.1) and 55.6% were female. Median delay in diagnosis was 10.9 months (range 1.0-72.0). The most frequent presenting symptoms were bone pain (100%) and fever (44.4%). Most patients had more than one lesion (median of 5, range 1-7). Most frequently affected bones were tibiofibula (88.9%) and femur (77.8%). The MRI characteristics mainly presented as bone edema and hyperintensity in bone marrow. Bone biopsy was conducted in 11 patients (61.1%) with inflammatory cells infiltration manifested as chronic osteomyelitis, and none showed bacterial infection or tumor. In treatment, non-steroid anti-inflamatory drugs (NSAIDs) is used as the first-line drug followed by steriods, methotexate (MTX), salazosulfadimidine (SASP), Bisphosphonates and TNF-α inhibitor. Two refractory cases received combination therapy with Bisphosphonates and TNF-α inhibitor, and achieved good therapeutic effect.

Conclusions: The present study described a multicenter series of CNO from south China and highlighted the clinical features, laboratory tests, imaging characteristics and treatment outcomes. Increasing awareness of this disease is important to decrease time to diagnosis, improve access to treatment, and reduce complications.
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http://dx.doi.org/10.1186/s12969-021-00657-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8722093PMC
January 2022

PAK3 promotes the metastasis of hepatocellular carcinoma by regulating EMT process.

J Cancer 2022 1;13(1):153-161. Epub 2022 Jan 1.

National Center for International Research of Bio-targeting Theranostics, Guangxi Key Laboratory of Bio-targeting Theranostics, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Talent Highland of Bio-targeting Theranostics, Guangxi Medical University, China.

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. The malignant biological behavior of HCC is closely related to epithelial-mesenchymal transition (EMT), and EMT plays an important role in the progression, migration and metastasis of HCC. P21-activated kinase 3 (PAK3) is a serine/threonine protein kinase, and PAK3 affects the EMT, proliferation, metastasis and invasion of HCC. In this study, the relationship between PAK3 and HCC was first analyzed by bioinformatics, and then, the expression of PAK3 in clinical samples was detected by immunohistochemistry (IHC), quantitative real-time PCR (qRT-PCR) and Western blotting. Subsequently, the expression of PAK3 was further confirmed in HCC cells. In addition, after the overexpression or knockdown of PAK3 in cells, the proliferation, migration and invasion abilities of these cells were assessed by Cell Counting Kit-8 (CCK-8), wound healing and Transwell assays, and the results were confirmed experiments in mice. In addition, we also verified that PAK3 affected the EMT and EMT-related pathway of HCC through qRT-PCR, Western blotting and immunofluorescence experiments. Through database analysis, we found that PAK3 was highly expressed in HCC patients and was positively correlated with tumor stage and grade, suggesting that PAK3 expression was closely related to HCC occurrence and development. We subsequently confirmed that PAK3 was overexpressed in HCC clinical samples and HCC cell lines and that PAK3 promoted the proliferation, migration and invasion of HCC cells . Finally, we found that PAK3 regulated EMT-related molecule expression and EMT-related TGF-β/smad signaling pathway. High expression of PAK3 enhances the invasion of HCC and regulates EMT, suggesting that PAK3 may be a potential target for the treatment of HCC.
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http://dx.doi.org/10.7150/jca.61918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692680PMC
January 2022

Associations of Gene Polymorphisms With Cognitive Function in Rural Chinese Population.

Front Aging Neurosci 2021 14;13:757992. Epub 2021 Dec 14.

Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Guangxi, China.

The study aimed to investigate the relationship between transcription factor EB () gene polymorphisms, including their haplotypes, and the cognitive functions of a selected population in Gongcheng County, Guangxi. A case-control study approach was used. The case group comprised 339 individuals with cognitive impairment, as assessed by their Mini-Mental State Examination scores; the control population also comprised 339 individuals who were matched by sex and age (± 5 years) in a 1:1 ratio. gene polymorphisms were genotyped in 678 participants (190 men and 488 women, aged 30-91 years) by using the Sequenom MassARRAY platform. Multifactorial logistic regression analysis showed that in the dominant model, the risk of developing cognitive impairment was 1.547 times higher in cases with the rs14063A allele (AG + AA) than in those with the GG genotype (adjusted odds ratio [OR] = 1.547, Bonferroni correction confidence interval = 1.021-2.345). Meanwhile, the presence of the rs1062966T allele (CT + TT) was associated with a lower risk of cognitive impairment in comparison with the presence of the CC genotype (adjusted OR = 0.636, Bonferroni correction confidence interval = 0.405-0.998). In the co-dominant model, the risk of developing cognitive impairment was 1.553 times higher in carriers of the rs14063AG genotype than in carriers of the GG genotype (adjusted OR = 1.553, Bonferroni correction confidence interval = 1.007-2.397). After the Bonferroni correction and adjustment for confounding factors, the association of rs1062966 with cognitive function persisted in the analyses stratified by education level. Ethnically stratified analysis showed a significant association between rs1062966 and cognitive function in the Yao population. The multilocus linkage disequilibrium analysis indicated that the identified single nucleotide polymorphisms were not inherited independently. The haplotype analysis suggested that the rs14063A-rs1062966C-rs2278068C-rs1015149T haplotype of the gene increased the risk of cognitive impairment ( < 0.05) and that the rs14063G-rs1062966T-rs2278068C-rs1015149C haplotype was associated with a reduced risk of cognitive impairment ( < 0.05). rs1062966 polymorphisms and their rs14063A-rs1062966C-rs2278068C-rs1015149T and rs14063G-rs1062966T-rs2278068C-rs1015149C haplotypes are genetic factors that may affect cognitive function among the rural Chinese population.
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http://dx.doi.org/10.3389/fnagi.2021.757992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8713571PMC
December 2021

Coronary catheterization via distal transradial access in patient with superficial radial artery: a case report.

BMC Cardiovasc Disord 2021 12 28;21(1):616. Epub 2021 Dec 28.

Heart Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Background: The routine radial artery (RA) puncture may fail when anatomical variation of the RA is encountered. Superficial radial artery (SRA) is one of the anatomic variants of the RA, with the incidence of about 1 to 1.5%. Recently, distal transradial access (dTRA) has emerged as a novel approach for coronary catheterization (CC), but performing CC through dTRA in patient with SRA has never been reported.

Case Presentation: A 57-year-old male was admitted to hospital due to intermittent chest pain for 4 days. He was diagnosed with unstable angina pectoris and planned to receive coronary angiography (CAG). Before the operation, the existence and course of SRA were confirmed by palpation and ultrasonography with color Doppler. We marked the puncture site under the guidance of ultrasonography and successfully performed CC through the dTRA during patient's hospitalization.

Conclusions: As far as we know, this is the first report that presents a case of SRA and percutaneous coronary intervention (PCI) treatment in which was successfully performed through dTRA. It is safe and feasible to perform CC via dTRA in case of SRA, and dTRA seems to be the preferred access.
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http://dx.doi.org/10.1186/s12872-021-02444-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714422PMC
December 2021

The chromosome-level genome provides insight into the molecular mechanism underlying the tortuous-branch phenotype of Prunus mume.

New Phytol 2021 Dec 3. Epub 2021 Dec 3.

Beijing Key Laboratory of Ornamental Plants, Germplasm Innovation & Molecular Breeding, National Engineering Research Centre for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of the Landscape Environment of the Ministry of Education, Key Laboratory of Genetics and Breeding of Forest Trees and Ornamental Plants of the Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing, 100083, China.

Plant with naturally twisted branches is referred to as a tortuous-branch plant, which have extremely high ornamental value due to their zigzag shape and the natural twisting of their branches. Prunus mume is an important woody ornamental plant. However, the molecular mechanism underlying this unique trait in Prunus genus is unknown. Here, we present a chromosome-level genome assembly of the cultivated P. mume var. tortuosa created using Oxford Nanopore combined with Hi-C scaffolding, which resulted in a 237.8 Mb genome assembly being anchored onto eight pseudochromosomes. Molecular dating indicated that P. mume is the most recently differentiated species in Prunus. Genes associated with cell division, development and plant hormones play essential roles in the formation of tortuous branch trait. A putative regulatory pathway for the tortuous branch trait was constructed based on gene expression levels. Furthermore, after transferring candidate PmCYCD genes into Arabidopsis thaliana, we found that seedlings overexpressing these genes exhibited curled rosette leaves. Our results provide insights into the evolutionary history of recently differentiated species in Prunus genus, the molecular basis of stem morphology, and the molecular mechanism underlying the tortuous branch trait and highlight the utility of multi-omics in deciphering the properties of P. mume plant architecture.
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http://dx.doi.org/10.1111/nph.17894DOI Listing
December 2021

Identification of Hub Genes in Hemifacial Microsomia: Evidence From Bioinformatic Analysis.

J Craniofac Surg 2021 Dec 2. Epub 2021 Dec 2.

Department of Maxillofacial Surgery Department of Auricular Reconstruction, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

Objective: This thesis addresses a neglected aspect of bioinformatics research of hemifacial microsomia (HFM). Existing research stops short of prediction based on big data. This study combines multiple databases to explore underlying pathogenesis using bioinformatic approach.

Methods: The research consisted of multiple bioinformatic methods, included pathogenic genes analyses, protein-protein interaction network construction, functional enrichment, and mining target genes related miRNA, for studying pathogenic genes of HFM.

Results: Total of 140 genes were identified as potential genes in the study. The protein-protein interaction networks for pathogenic genes were constructed, which contained 138 nodes and 243 edges with RAF1, MAP2K1, MAP2K2, MAPK3, MAPK1, EGFR, BRAF, LMNA, ESPR1, and SFN as the hub genes. These genes were discovered significantly enriched in MAPK pathway. Besides, the whole of interactions between miRNAs and the top 5 hub genes were revealed.

Conclusions: Our results indicated that occurrence of HFM is attributed to a variety of genes. Furthermore, the interactions of pathogenic genes were further elucidated by using bioinformatics approach. It reveals the MAPK pathway play an essential role in its pathogenesis. It may provide a novel perspective on better understanding the pathogenesis and more accurate early screening of HFM.
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http://dx.doi.org/10.1097/SCS.0000000000008164DOI Listing
December 2021

The O2-ZmGRAS11 transcriptional regulatory network orchestrates the coordination of endosperm cell expansion and grain filling in maize.

Mol Plant 2021 Nov 27. Epub 2021 Nov 27.

National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology & Ecology, Shanghai 200032, China. Electronic address:

Maize (Zea mays) endosperm filling is coordinated with cell expansion to enlarge the grain size, but the mechanism coupling the two processes is poorly understood. Starchy endosperm cells basically contain no visible vacuoles for cell expansion. During grain filling, efficient synthesis of storage compounds leads to reduced cytoplasm and thus lowered cell turgor pressure. Although bioactive gibberellins (GAs) are essential for cell expansion, they accumulate at a low level at this stage. In this study, we identified an endosperm-specific GRAS domain-containing protein (ZmGRAS11) that lacks the DELLA domain and promotes cell expansion in the filling endosperm. The zmgras11 loss-of-function mutants showed normal grain filling but delayed cell expansion, thereby resulting in reduced kernel size and weight. Overexpression of ZmGRAS11 led to larger endosperm cells and therefore increased kernel size and weight. Consistent with this, ZmGRAS11 positively regulates the expression of ZmEXPB12, which is essential for cell expansion, at the endosperm filling stage. Moreover, we found that Opaque2 (O2), a central transcription factor that regulates endosperm filling, could directly bind to the promoter of ZmGRAS11 and activate its expression. Taken together, these results suggest that endosperm cell expansion is coupled with endosperm filling, which is orchestrated by the O2-ZmGRAS11-centered transcriptional regulatory network. Our findings also provide potential targets for maize yield improvement by increasing the storage capacity of endosperm cells.
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http://dx.doi.org/10.1016/j.molp.2021.11.013DOI Listing
November 2021

Fungicide Difenoconazole Induced Biochemical and Developmental Toxicity in Wheat ( L.).

Plants (Basel) 2021 Oct 26;10(11). Epub 2021 Oct 26.

Department of Biology, East Carolina University, Greenville, NC 27858, USA.

Difenoconazole is one of the most commonly used fungicides to prevent and treat plant diseases caused by certain fungi. Due to increasing usage, more difenoconazole has been released into the environment and caused environment pollution. However, the potential impact of difenoconazole on plant growth and development and its involved mechanism are unclear. In this study, we discovered that difenoconazole exposure significantly inhibited plant growth, evidenced by the decrease in root dry weight, total root length, and surface area by 20-70%, 43-73%, and 26-66%, respectively, under different regimes of treatment concentrations and periods. Difenoconazole exposure also significantly inhibited shoot growth and development by decreasing 33-61% of the shoot dry weight and 50-65% of the leaf area. Difenoconazole exposure induced plant leaf cells to generate more ROS (O and HO) and MDA, which resulted in a decreased chlorophyll content and then inhibited leaf photosynthesis. Difenoconazole exposure also induced the activities of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (G-POD), and ascorbate peroxidase (APX) in the roots and leaves of the wheat seedlings. SOD and APX activities were higher and more stable in the roots than those in the leaves. Based on our study, plant roots exhibited a more pronounced superoxide radical scavenging ability than plant leaves. In summary, difenoconazole exposure caused oxidative stress, reduced chlorophyll biosynthesis and functions, and then inhibited wheat plant growth and development.
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http://dx.doi.org/10.3390/plants10112304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621447PMC
October 2021

Strong histamine torsion Raman spectrum enables direct, rapid, and ultrasensitive detection of allergic diseases.

iScience 2021 Nov 30;24(11):103384. Epub 2021 Oct 30.

National Laboratory of Solid States Microstructures and Key Laboratory of Modern Acoustics, MOE, Institute of Acoustics, Nanjing University, Nanjing 210093, China.

Allergic diseases are closely related to degranulation and release of histamine and difficult to diagnose because non-allergic diseases also exhibit the same clinical symptoms as allergy. Here, we report direct, rapid, and ultrasensitive detection of histamine using low-frequency molecular torsion Raman spectroscopy. We show that the low-frequency (<200 cm) Raman spectral intensities are stronger by one order of magnitude than those of the high-frequency Raman ones. Density functional theory calculation and nuclear magnetic resonance spectroscopy identify the strong spectral feature to be from torsions of carbon-carbon single bonds, which produce large variations of the polarizability densities in the imidazole ring and ethyl amino side chain. Using an omniphobic substrate and surface plasmonic effect of [email protected] nanoparticles, the detection limit (signal-noise ratio >3) of histamine reaches 10 g/L in water and 10 g/L in serum. This scheme thus opens new lines of inquiry regarding the clinical diagnosis of allergic diseases.
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http://dx.doi.org/10.1016/j.isci.2021.103384DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605255PMC
November 2021

Tocilizumab in Systemic Juvenile Idiopathic Arthritis: Response Differs by Disease Duration at Medication Initiation and by Phenotype of Disease.

Front Pediatr 2021 8;9:735846. Epub 2021 Nov 8.

Department of Rheumatology and Immunology, Children's Hospital of Chongqing Medical University, Chongqing, China.

We performed a single-center retrospective study to determine the different efficacy of tocilizumab (TCZ) in the early and late stages and in three phenotypic subgroups (monocyclic, polycyclic, and persistent) of systemic juvenile idiopathic arthritis (sJIA). Clinical and serological parameters of 77 sJIA patients treated by TCZ were collected from November 1, 2013 to May 1, 2019. Patients were grouped based on the duration group A < 6 months ( = 41) and group B > 6 months ( = 36) and divided into three phenotypes: monocyclic ( = 12), polycyclic ( = 14), and persistent ( = 51) course. At baseline, group A had pronounced ESR, fever less active arthritis than group B ( < 0.05). After 12 weeks of therapy, TCZ alleviated fever, ESR, CRP, and systemic-onset juvenile arthritis disease activity score-27 (sJADAS27) in both group A and group B (>0.05), while the efficacy of TCZ in relieving active arthritis in group A was better than that in group B (<0.05). After 1 year of TCZ therapy, it showed that patients with monocyclic phenotype had the highest clinical response rate (91.7%, odds ratio = 0, 95% CI: 24-24, = 0.00), followed by the polycyclic (28.6%, odds ratio = 2.1, 95% CI: 10.5-18.8, = 0.00) and the persistent course (9.8%, odds ratio = 1.2, 95% CI: 9.5-13.8, = 0.00). TCZ can quickly relieve fever and inflammation, especially when patients have less active arthritis with shorter disease duration. The long-term efficacy of TCZ is related to the phenotypes, among which the monocyclic is the best, and the persistent is the worst.
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http://dx.doi.org/10.3389/fped.2021.735846DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606744PMC
November 2021

Carbon Nanotube-Based Field-Effect Transistor-Type Sensor with a Sensing Gate for Ppb-Level Formaldehyde Detection.

ACS Appl Mater Interfaces 2021 Dec 17;13(47):56309-56319. Epub 2021 Nov 17.

Hunan Institute of Advanced Sensing and Information Technology, Xiangtan University, Xiangtan 411105, P. R. China.

The detection of harmful trace gases, such as formaldehyde (HCHO), is a technical challenge in the current gas sensor field. The weak electrical signal caused by trace amounts of gases is difficult to be detected and susceptible to other gases. Based on the amplification effect of a field-effect transistor (FET), a carbon-based FET-type gas sensor with a gas-sensing gate is proposed for HCHO detection at the ppb level. Semiconducting carbon nanotubes (s-CNTs) and a catalytic metal are chosen as channel and gate materials, respectively, for the FET-type gas sensor, which makes full use of the respective advantages of the channel transport layer and the sensitive gate layer. The as-prepared carbon-based FET-type gas sensor exhibits a low detection limit toward HCHO up to 20 ppb under room temperature (RT), which can be improved to 10 ppb by a further heating strategy. It also exhibits a remarkable elevated recovery rate from 80 to 97% with almost no baseline drift (2%) compared to the RT condition, revealing excellent reproducibility, stability, and recovery. The role of sensitive function in the FET-type gas sensor is performed by means of an independent gas-sensing gate, that is, the independence of the sensitive gate and the electron transmission channel is the main reason for its high sensitivity detection. We hope our work can provide an instructive approach for designing high-performance formaldehyde sensor chips with on-chip integration potential.
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http://dx.doi.org/10.1021/acsami.1c17044DOI Listing
December 2021

Correlation between urinary contents of some metals and fasting plasma glucose levels: A cross-sectional study in China.

Ecotoxicol Environ Saf 2021 Nov 12;228:112976. Epub 2021 Nov 12.

Department of Environmental and Occupational Health, School of Public Health, Guangxi Medical University, Nanning 530021, Guangxi, China. Electronic address:

Many metals are involved in the pathogenesis of diabetes, but most of existing studies focused on single metals. The study of mixtures represents real-life exposure scenarios and deserves attention. This study aimed to explore the potential relationship of urinary copper (Cu), zinc (Zn), arsenic (As), selenium (Se), and strontium (Sr) contents with fasting plasma glucose (FPG) levels in 2766 participants. The levels of metals in urine were determined by inductively coupled plasma-mass spectrometry. We used linear regression models and the Bayesian kernel machine regression (BKMR) to evaluate the association between metals and FPG levels. In the multiple metals linear regression, Zn (β = 0.434), Se (β = 0.172), and Sr (β = -0.143) showed significant association with FPG levels (all P < 0.05). The BKMR model analysis showed that the results of single metal association were consistent with the multiple metals linear regression. The mixture of five metals had a positive over-all effect on FPG levels, and Zn (PIP = 1.000) contributed the most to the FPG levels. Cu and As were negatively correlated with FPG levels in women. The potential interaction effect between Cu and Sr was observed in participants aged ≥ 60 years old (P = 0.035). In summary, our results suggested that multiple metals in urine are associated with FPG levels. Further studies are needed to confirm these findings and clarify the underlying mechanisms.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112976DOI Listing
November 2021

Daily moral identity: Linkages with integrity and compassion.

J Pers 2021 Nov 12. Epub 2021 Nov 12.

Department of Psychology and Neuroscience, Baylor University, Waco, Texas, USA.

Objective: The present study investigated how much variability in moral identity scores is attributable to individual differences that are stable over time and how much variability reflects daily fluctuations.

Method: Participants (N = 138, M age = 25.11 years, SD = 10.77; 82% female) were asked to report the self-importance of three moral attributes (being honest, fair, and caring) once a day for 50 consecutive days. Ratings were decomposed into between- and within-person variability and analyzed in relation to individuals' self-reported feelings of integrity and compassion using hierarchical linear modelling.

Results: Daily measures of moral identity exhibited more between- than within-person variability (64% vs. 36%). Furthermore, feelings of integrity and compassion were more strongly positively correlated with moral identity on the inter-individual level than the intra-individual level.

Conclusion: Overall, findings suggest that moral identity has both trait- and state-like characteristics and might be best conceptualized as a characteristic adaptation evidencing both stability and change.
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http://dx.doi.org/10.1111/jopy.12689DOI Listing
November 2021

The Pathogenic Mechanism of Carotid Web Causing the Recurrent Stroke.

Neurol India 2021 Sep-Oct;69(5):1483-1484

Department of Neurology, LiShui People's Hospital, Zhejiang Province, China.

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http://dx.doi.org/10.4103/0028-3886.329610DOI Listing
November 2021

Carbon Nanotube Field-Effect Transistor Biosensor for Ultrasensitive and Label-Free Detection of Breast Cancer Exosomal miRNA21.

Anal Chem 2021 11 8;93(46):15501-15507. Epub 2021 Nov 8.

School of Laboratory Medicine, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan 430065, China.

Tumor-derived exosomal miRNAs may have important functions in the onset and progression of cancers and are potential biomarkers for early diagnosis and prognosis monitoring. Yet, simple, sensitive, and label-free detection of exosomal miRNAs remains challenging. Herein, an ultrasensitive, label-free, and stable field-effect transistor (FET) biosensor based on a polymer-sorted high-purity semiconducting carbon nanotube (CNT) film is reported to detect exosomal miRNA. Different from conventional CNT FETs, the CNT FET biosensors employed a floating gate structure using an ultrathin YO as an insulating layer, and assembled Au nanoparticles (AuNPs) on YO as linkers to anchor probe molecules. A thiolated oligonucleotide probe was immobilized on the AuNP surface of the sensing area, after which miRNA21 was detectable by monitoring the current change before and after hybridization between the immobilized DNA probe and target miRNA. This method achieved both high sensitivity (LOD: 0.87 aM) and high specificity. Furthermore, the FET biosensor was employed to test clinical plasma samples, showing significant differences between healthy people and breast cancer patients. The CNT FET biosensor shows the potential applications in the clinical diagnosis of breast cancer.
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http://dx.doi.org/10.1021/acs.analchem.1c03573DOI Listing
November 2021

Multicentric Carpo-Tarsal Osteolysis Syndrome Mimicking Juvenile Idiopathic Arthritis: Two Case Reports and Review of the Literature.

Front Pediatr 2021 15;9:745812. Epub 2021 Oct 15.

Department of Rheumatology and Immunology, Children's Hospital of Chongqing Medical University, Chongqing, China.

Multicentric carpo-tarsal osteolysis syndrome (MCTO) is a rare skeletal disorder commonly caused by MAF bZIP transcription factor B () mutation. Clinically, it is characterized by aggressive osteolysis, which mainly affects the carpal tarsal bones, and is frequently associated with progressive nephropathy. Since the painful swelling and motion limitation on the wrists and/or ankles of MCTO mimics those of juvenile idiopathic arthritis (JIA), very often, MCTO is misdiagnosed as JIA. Here, we report two MCTO patients initially diagnosed with JIA but showed no response to treatment: P1, with a medical history of more than 10 years, was presented with a typical triad of arthritis-osteolysis-nephropathy; while P2 showed oligoarthritis. Gene tests were then taken and revealed a novel mutation, p.P63Q, and a previously reported conversion, p.S54L, in the gene. We also summarized the clinical and genetic features of a cohort containing 49 genetically confirmed MCTO patients. All 51 gene-confirmed MCTO cases (49 identified from the literature plus two patients identified herein) developed the disease during childhood. The median delay in diagnosis was 3.83 years (0-35 years). All cases presented bony lesions, and two-thirds had secondary renal lesions (32/48; three unknown), half of which (16/32) progressed into renal failure. Almost two-thirds (34/51), 75% (38/51), and 71% (36/51) of patients had no record of eye problems, facial abnormalities, and other manifestations. Most were misdiagnosed as JIA but didn't respond to treatment. Based on our experience, we suggest that clinicians should comprehensively evaluate the involvement of multiple systems in JIA patients, especially the kidney and eyes. And for JIA patients who underwent more than 3-month treatment with Bio-DMARD, genetic tests are recommended when they show little/no clinical and imaging changes, their high disease activity remains, and their disease activity remission is <50%, especially when combined with a triad of arthritis-osteolysis-nephropathy.
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http://dx.doi.org/10.3389/fped.2021.745812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554157PMC
October 2021

Clinical and CT Radiomics Nomogram for Preoperative Differentiation of Pulmonary Adenocarcinoma From Tuberculoma in Solitary Solid Nodule.

Front Oncol 2021 12;11:701598. Epub 2021 Oct 12.

Department of Thoracic Surgery, Beilun Second People's Hospital, Zhejiang, China.

Aim: To investigate clinical and computed tomography (CT) radiomics nomogram for preoperative differentiation of lung adenocarcinoma (LAC) from lung tuberculoma (LTB) in patients with pulmonary solitary solid nodule (PSSN).

Materials And Methods: A total of 313 patients were recruited in this retrospective study, including 96 pathologically confirmed LAC and 217 clinically confirmed LTB. Patients were assigned at random to training set (n = 220) and validation set (n = 93) according to 7:3 ratio. A total of 2,589 radiomics features were extracted from each three-dimensional (3D) lung nodule on thin-slice CT images and radiomics signatures were built using the least absolute shrinkage and selection operator (LASSO) logistic regression. The predictive nomogram was established based on radiomics and clinical features. Decision curve analysis was performed with training and validation sets to assess the clinical usefulness of the prediction model.

Results: A total of six clinical features were selected as independent predictors, including spiculated sign, vacuole, minimum diameter of nodule, mediastinal lymphadenectasis, sex, and age. The radiomics nomogram of lung nodules, consisting of 15 selected radiomics parameters and six clinical features showed good prediction in the training set [area under the curve (AUC), 1.00; 95% confidence interval (CI), 0.99-1.00] and validation set (AUC, 0.99; 95% CI, 0.98-1.00). The nomogram model that combined radiomics and clinical features was better than both single models (p < 0.05). Decision curve analysis showed that radiomics features were beneficial to clinical settings.

Conclusion: The radiomics nomogram, derived from unenhanced thin-slice chest CT images, showed favorable prediction efficacy for differentiating LAC from LTB in patients with PSSN.
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http://dx.doi.org/10.3389/fonc.2021.701598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546326PMC
October 2021

Synthesis of graphene oxide/polyethyleneimine sponge and its performance in the sustainable removal of Cu(II) from water.

Sci Total Environ 2022 Feb 26;806(Pt 3):151258. Epub 2021 Oct 26.

School of Environmental Science and Engineering, Shanghai Jiao Tong University, No. 800, Dongchuan Road, Shanghai 200240, China. Electronic address:

Modification of graphene oxide (GO) with polyethyleneimine (PEI) has been studied to develop a GO/PEI sponge material that not only performs well in the adsorption of Cu(II) but also is easily separated from water. The results showed that GO had excellent affinity for PEI, and GO/PEI prepared at pH 9.0 using PEI with a MW of 70,000 was shown to be a good adsorbent for Cu(II). This GO/PEI was characterized with SEM, XRD, XPS, FTIR and TG analyses and was investigated for Cu(II) adsorption further. The adsorption isotherm data of Cu(II) were fitted well with the Langmuir model, from which the maximum adsorption of GO/PEI was calculated to be 150.9 mg/g at pH 5.5. This was much higher than that of GO and two commercial resins. GO/PEI showed high selectivity towards Cu(II). In GO/PEI, the contributions of amino groups on PEI and negative charges on GO were 79.2% and 20.8%, respectively. Cu(II) adsorption on GO/PEI decreased with decreasing pH, and 1 M HCl caused nearly complete desorption of the adsorbed Cu(II). Experimental results of five cycles of adsorption-desorption indicated that this material could be reused. Column studies showed that GO/PEI performed well in terms of both Cu(II) adsorption and stability in water.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151258DOI Listing
February 2022

Populations at high risk of cervical cancer in Guangxi Province: Findings from two screening projects in a minority area of South China.

J Med Screen 2021 Oct 25:9691413211039254. Epub 2021 Oct 25.

School of Public Health, 74626Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China.

Objective: To analyse the positive rates of low-grade (LSIL) and high-grade (HSIL) squamous intraepithelial lesions, and cervical cancer (CC), and identify groups at high risk for CC in Guangxi.

Setting: CC screening options in Guangxi, which is the only minority autonomous area in South China, include the National Cervical Cancer Screening Project (NCCSP) and physical examination (PE).

Methods: This study was based on PE and NCCSP sample data obtained from 2012 to 2019. We calculated the positive rates of LSIL, HSIL, and CC; analysed the adjusted odds ratio (aOR) and 95% confidence intervals (CI) of the variables in multivariate logistic regression; and subsequently identified groups at high risk for CC.

Results: The positive rates of LSIL, HSIL, and CC for the total of 873,880 samples were 1.89%, 0.60%, and 0.03%, respectively. Females over 64 years of age (vs. 50-64; aOR = 2.05; 95% CI, 1.71-2.46; < 0.001) and those from urban (vs. rural; aOR = 1.66; 95% CI, 1.57-1.76; < 0.001), minority (vs. non-minority; aOR = 1.24; 95% CI, 1.13-1.35; < 0.001), and coastal (vs. inland; aOR = 1.15; 95% CI, 1.06-1.25;  = 0.001) areas were associated with a high risk of HSIL. Females over 64 (vs. 50-64; aOR = 4.37; 95% CI, 2.88-6.63;  < 0.001) and those from urban (vs. rural; aOR = 3.05; 95% CI, 2.36-3.95;  < 0.001) areas were significantly associated with a high risk of CC.

Conclusion: Females from urban areas in Guangxi are at high risk for CC. Public health strategies should focus on high-risk populations.
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http://dx.doi.org/10.1177/09691413211039254DOI Listing
October 2021

Prevalence and spatial heterogeneity of Trichomonas vaginalis infection among the female population and association with climate in Guangxi Zhuang autonomous region, Southern China.

Acta Trop 2022 Jan 21;225:106204. Epub 2021 Oct 21.

School of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region, China; School of Public Health, Guilin Medical University, 20 Lequn Road, Guilin, Guangxi Zhuang Autonomous Region, China; Key Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education, 10 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region, China; Guangxi Health Commission Key Laboratory of Entire Lifecycle Health and Care, 20 Lequn Road, Guilin, Guangxi Zhuang Autonomous Region, China. Electronic address:

Background: Two cervical cancer screening (CCS) projects have been ongoing for years in Guangxi Zhuang autonomous region (Guangxi), and some Trichomonas vaginalis infection (TVI) cases have been found as an opportunistic finding. This study aimed to identify the high-risk population and expound the spatial epidemiological features of TVI in Guangxi.

Methods: This study was based on CCS from 2012 to 2019. Adjusted odds ratio (AOR), and spatial analyses were used to identify the high-risk subgroups, as well as to depict the spatial epidemiological feature and its relationship with meteorological factors.

Results: The infection rate of TVI was 0.38% in 873,880 samples. Significant association with a high risk of TVI was found in the following: females aged 40-49 years (aOR=4.464; 95% CI, 3.359-5.932; p<0.001), aged 50-59 years (aOR=3.169; 95% CI, 2.370-4.237; p<0.001), from urban (aOR=1.577; 95% CI, 1.471-1.691; p<0.001), from minority areas (aOR=1.183; 95% CI, 1.060-1.320; p=0.003), areas with GPD <41,500 CNY (aOR=1.191; 95% CI, 1.106-1.282; p<0.001), and inland areas (aOR=1.520; 95% CI, 1.339-1.726; p<0.001). Counties with higher infection rate were concentrated in northwest Guangxi's mountainous area (Z-score=3.9656, p<0.001), in the upper reaches of the Hongshui River and Yu River, and with a significant spatial autocorrelation (Moran's I=0.581, p=0.002). Spatial error model showed significantly negative regressions among temperature (B=-0.295, p=0.002), annual temperature range (B=-0.295, p=0.002), and TVI spatial distribution.

Conclusion: The spatial clustering and disparity of TVI in northwest Guangxi warrant further study, and meteorological conditions may play an important role in TVI in northwest Guangxi.
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http://dx.doi.org/10.1016/j.actatropica.2021.106204DOI Listing
January 2022

A New Perspective on the Role of Glutamine Synthetase in Nitrogen Remobilization in Wheat ( L.).

Int J Mol Sci 2021 Oct 14;22(20). Epub 2021 Oct 14.

Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450000, China.

Glutamine synthetase (GS), a key enzyme in plant nitrogen metabolism, is closely related to nitrogen remobilization. However, how GS isoforms participate in nitrogen remobilization remains unclear. Here, the spatiotemporal expression of the gene family after anthesis was investigated, and the results showed that TaGS1;1 was mainly encoded by , while the other isozymes were mainly encoded by localized on the A and D subgenomes. had the highest expression level, especially in rachis and peduncle. Furthermore, immunofluorescence showed TaGS1;2 was located in the phloem of rachis and peduncle. GUS (β-glucuronidase) staining confirmed that activity was mainly present in the vascular system of leaves, roots, and petal of . Ureides, an important transport form of nitrogen, were mainly synthesized in flag leaves and transported to grains through the phloem of peduncle and rachis during grain filling. , which encodes the enzyme that degrades ureides to release NH, had a higher expression in rachis and peduncle and was synchronized with the increase in NH concentration in phloem, indicating that NH in phloem is from ureide degradation. Taking the above into account, TaGS1;2, which is highly expressed in the phloem of peduncle and rachis, may participate in N remobilization by assimilating NH released from ureide degradation.
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http://dx.doi.org/10.3390/ijms222011083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539157PMC
October 2021

Geographical distinctions of longevity indicators and their correlation with climatic factors in the area where most Chinese Yao are distributed.

Int J Biometeorol 2022 Jan 19;66(1):97-110. Epub 2021 Oct 19.

School of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region, China.

Longevity research is a hot topic in the health field. Considerable research focuses on longevity phenomenon in Bama Yao Autonomous County, which has a typical karst landform and is located in Southwest China. This study aims to illustrate the spatial feature of longevity indicators in other Yao areas, to analyze the correlation between climatic factors and longevity indicators, and to provide new clues and targets for further longevity studies. We collect and integrate population, climate, and terrain data into a spatial database. The main analysis methods include spatial autocorrelation, high/low clustering, and multiscale geographically weighted regression (MGWR). Two longevity clusters are identified in Guijiang River Basin (longevity index (LI%): 2.49 ± 0.63) and Liujiang River Basin (LI%: 2.13 ± 0.60). The spatial distribution of longevity indicators is autocorrelative (Moran's I = 0.652, p < 0.001) and clustered significantly (Z score = 4.268, p < 0.001). MGWR shows that the atmospheric pressure significantly affects the spatial distribution of LI% (estimate value (EV) =  - 0.566, p = 0.012), centenarity index (CI%) (EV =  - 0.425, p = 0.007), UC (EV =  - 0.502, p = 0.006), and CH (EV =  - 0.497, p = 0.007). Rainfall significantly affects the spatial distribution of LI% (EV = 0.300, p = 0.003) and CI% (EV =  - 0.191, p = 0.016). The spatial distribution of the main longevity indicators shows significant heterogeneity and autocorrelation, and they cluster in the Guijiang River and Liujiang River basins. Atmospheric pressure and rainfall may contribute to the longevity phenomenon through complex mechanisms. The longevity phenomenon in the Yao nationality in Guijiang River Basin requires further study to improve our understanding of the health effect of meteorological, environmental, and social conditions on longevity.
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http://dx.doi.org/10.1007/s00484-021-02195-wDOI Listing
January 2022
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