Publications by authors named "Zhiyong Li"

578 Publications

Radiomics for identifying lung adenocarcinomas with predominant lepidic growth manifesting as large pure ground-glass nodules on CT images.

Authors:
Ziqi Xiong Yining Jiang Di Tian Jingyu Zhang Yan Guo Guosheng Li Dongxue Qin Zhiyong Li

PLoS One 2022 24;17(6):e0269356. Epub 2022 Jun 24.

Department of Radiology, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.

Purpose: To explore the value of radiomics in the identification of lung adenocarcinomas with predominant lepidic growth in pure ground-glass nodules (pGGNs) larger than 10 mm.

Methods: We retrospectively analyzed CT images of 204 patients with large pGGNs (≥ 10 mm) pathologically diagnosed as minimally invasive adenocarcinomas (MIAs), lepidic predominant adenocarcinomas (LPAs), and non-lepidic predominant adenocarcinomas (NLPAs). All pGGNs in the two groups (MIA/LPA and NLPA) were randomly divided into training and test cohorts. Forty-seven patients from another center formed the external validation cohort. Baseline features, including clinical data and CT morphological and quantitative parameters, were collected to establish a baseline model. The radiomics model was built with the optimal radiomics features. The combined model was developed using the rad_score and independent baseline predictors. The performance of the models was evaluated using the area under the receiver operating characteristic curve (AUC) and compared using the DeLong test. The differential diagnosis performance of the models was compared with three radiologists (with 20+, 10+, and 3 years of experience) in the test cohort.

Results: The radiomics (training AUC: 0.833; test AUC: 0.804; and external validation AUC: 0.792) and combined (AUC: 0.849, 0.820, and 0.775, respectively) models performed better for discriminating than the baseline model (AUC: 0.756, 0.762, and 0.725, respectively) developed by tumor location and mean CT value of the whole nodule. The DeLong test showed that the AUCs of the combined and radiomics models were significantly increased in the training cohort. The highest AUC value of the radiologists was 0.600.

Conclusion: The application of CT radiomics improved the identification performance of lung adenocarcinomas with predominant lepidic growth appearing as pGGNs larger than 10 mm.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0269356PLOS
June 2022

Long anterior lens zonules with retinal stripes: a case report.

Authors:
Xiaolu Cao Jiayin Qin Zhiyong Li Peipei Jia Beibei Gao Lin Feng Wei Wang

BMC Ophthalmol 2022 Jun 23;22(1):274. Epub 2022 Jun 23.

Hebei Ophthalmology Key Lab, Hebei Eye Hospital, No. 399, Quanbei East Street, Xingtai, 054000, Hebei, China.

Background: Long anterior lens zonules   (LAZs) is a rare disease that was mostly conducted among African Americans. Through the observation of a Chinese patient, we discoverd that the disease may show different characteristics in Asians.

Case Presentation: A patient with vision loss due to a macular hole was found to have several special clinical signs during vitrectomy combined with phacoemulsification and intraocular lens implantation surgery in our hospital, including radially oriented lines on the anterior capsule with pigment, a shallow anterior chamber, slightly high intraocular pressure, and radial retinal stripes in the peripheral retina. Finally, he was diagnosed with long anterior lens zonule syndrome.

Conclusion: Clinicians need to pay more attention to the rare disease LAZs. It is important to tear the appropriate size of the anterior capsule so as to avoid radial capsular tearing and intraocular lens dislocation.
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http://dx.doi.org/10.1186/s12886-022-02496-7DOI Listing
June 2022

Case Report: Multimodal Imaging Guides the Management of an Eosinophilic Leukemia Patient With Eosinophilic Myocarditis and Intracardiac Thrombus.

Authors:
Jinping Si Xinxin Zhang Na Chen Fangfang Sun Ping Du Zhiyong Li Di Tian Xiuli Sun Guozhen Sun Tao Cong Xuemei Du Ying Liu

Front Cardiovasc Med 2022 3;9:903323. Epub 2022 Jun 3.

Department of Cardiology, First Affiliated Hospital of Dalian Medical University, Dalian, China.

Background: Eosinophilic leukemia (EL) is a rare, serious and potentially life-threatening condition characterized by the overproduction of eosinophils leading to tissue eosinophilic infiltration and damage. Although multiple organ systems may be involved, progressive eosinophilic myocarditis (EM) is the most common cause of morbidity and mortality. Early diagnosis and follow-up surveillance combined with multimodal imaging are crucial for appropriate treatment of EM.

Case Summary: It's a rare case of EL with EM and intracardiac thrombus in a 59-year-old patient who presented with asthenia for 3 weeks. Full blood count analysis indicated significant eosinophilia. Bone marrow aspirate revealed dysplastic eosinophilia and a FIP1L1-PDGFRA fusion gene (4q12) was detected, confirming EL. Echocardiography revealed EM with intracardiac thrombus. This was later confirmed by cardiac magnetic resonance imaging. The patient was commenced on imatinib and prednisolone and good clinical response was obtained. Through 18F-FAPI PET/CT imaging, we obtained visualization of fibroblast activation changes in the early stage of cardiac structure remodeling. With anti-fibrotic therapy after heart failure, the patient achieved a good clinical response.

Conclusion: This case demonstrates visualization of fibroblast activation after EM. Multimodality imaging can provide early diagnosis and may guide tailored antifibrotic therapy in early stage of EM.
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http://dx.doi.org/10.3389/fcvm.2022.903323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204136PMC
June 2022

Prediction of Plant Resistance Proteins Based on Pairwise Energy Content and Stacking Framework.

Authors:
Yifan Chen Zejun Li Zhiyong Li

Front Plant Sci 2022 31;13:912599. Epub 2022 May 31.

College of Computer Science and Electronic Engineering, Hunan University, Changsha, China.

Plant resistance proteins (R proteins) recognize effector proteins secreted by pathogenic microorganisms and trigger an immune response against pathogenic microbial infestation. Accurate identification of plant R proteins is an important research topic in plant pathology. Plant R protein prediction has achieved many research results. Recently, some machine learning-based methods have emerged to identify plant R proteins. Still, most of them only rely on protein sequence features, which ignore inter-amino acid features, thus limiting the further improvement of plant R protein prediction performance. In this manuscript, we propose a method called StackRPred to predict plant R proteins. Specifically, the StackRPred first obtains plant R protein feature information from the pairwise energy content of residues; then, the obtained feature information is fed into the stacking framework for training to construct a prediction model for plant R proteins. The results of both the five-fold cross-validation and independent test validation show that our proposed method outperforms other state-of-the-art methods, indicating that StackRPred is an effective tool for predicting plant R proteins. It is expected to bring some favorable contribution to the study of plant R proteins.
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http://dx.doi.org/10.3389/fpls.2022.912599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194944PMC
May 2022

First-line PD-1 Inhibitor & EGFR Blockade & Platinum-based Chemotherapy (PEP) for Stage IV Penile Cancer.

Authors:
Ru Yan Huali Ma Lijuan Jiang Shengjie Guo Yanxia Shi Xinan Sheng Yijun Zhang Philippe E Spiess Tingyu Liu Ting Xue Xiaofeng Chen Zhiyong Li Xin An Kai Yao Fangjian Zhou Hui Han

BJU Int 2022 Jun 15. Epub 2022 Jun 15.

Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, China.

Objectives: To evaluate the anti-tumor activity and the safety of anti-PD-1 antibody plus EGFR blockade combined with platinum-based chemotherapy (PEP) as first-line therapy for stage IV PSCC.

Patients And Methods: Retrospective review of 17 patients with stage IV PSCC undergoing first-line PEP at Sun Yat-sen University Cancer Center between January 2018 and September 2021. Clinical responses were assessed by the RECIST version 1.1. Progression-free survival (PFS) and overall survival (OS) were estimated using the Kaplan-Meier method. Adverse events (AEs) were graded by the CTCAE version 5.0.

Results: Among 17 patients who received first-line PEP, 13 (76.5%) were observed to have partial responses. Twelve patients received subsequently consolidated surgery, and 10 (83.3%) of them achieved pN0 status, of which six locally advanced PSCC achieved pathological complete response. The median follow-up time was 24.87 months (range 3.63-29.40). Both median PFS and OS were not reached, with 2-year PFS and OS rates being 68.4% (95%CI 48.7-96.1) and 62.9% (95%CI 41.6-95), respectively. Eight (47.1%) patients suffered grade 3 or 4 treatment-related AEs. No grade 5 AEs or death associated with treatment was observed.

Conclusions: PEP showed promising anti-tumor activity, acceptable toxicity, and satisfying long-term survival for stage IV PSCC. Larger clinical trials are needed to validate our findings.
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http://dx.doi.org/10.1111/bju.15828DOI Listing
June 2022

Improving Trendelenburg position effectiveness by varying cardiopulmonary bypass flow.

Authors:
Raymond Ho Charles McDonald Jo P Pauls Zhiyong Li

Perfusion 2022 Jun 15:2676591221108810. Epub 2022 Jun 15.

School of Mechanical, Medical and Process Engineering, Faculty of Engineering, 1969Queensland University of Technology (QUT), Brisbane, QLD, Australia.

Introduction: Trendelenburg position (TP) is used to transport gaseous emboli away from the cerebral region during cardiac surgery. However, TP effectiveness has not been fully considered when combined with varying the cardiopulmonary bypass (CPB) flow. This study simulated the supine and TP at different pump flows and assessed the trapped emboli and embolic load entering the aortic arch branch arteries (AABA).

Methods: A computational fluid dynamics (CFD) approach used a centrally cannulated adult patient-specific aorta model replicating a CPB circuit. Air emboli of 0.1 mm, 0.5 mm, and 1.0 mm ( = 700 each) were injected into the aorta placed in the supine position (0°) and the TP (-20°) at 2 L/min and 5 L/min. The number of emboli entering the AABA were compared. An aortic phantom flow experiment was performed to validate air bubble behaviour.

Results: TP at 5 L/min had the lowest 0.1 mm mean (±SD) embolic load compared to the supine 2 L/min (55.3 ± 30.8 vs 64.3 ± 35.4). For both the supine and TP, the lower flow of 2 L/min had the highest number of simulated trapped emboli in higher elevated regions than at 5 L/min (541 ± 185 and 548 ± 191 vs 520 ± 159 and 512 ± 174), respectively. The flow experiment demonstrated that 2 L/min promoted bubble coalescence and high amounts of trapped emboli and 5 L/min transported air emboli away from the AABA.

Conclusions: TP effectiveness was improved by using CPB flow to manage air emboli. These results provide insights for predicting emboli behaviour and improving emboli de-airing procedures.
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http://dx.doi.org/10.1177/02676591221108810DOI Listing
June 2022

A visible light/heat responsive covalent organic framework for highly efficient and switchable proton conductivity.

Authors:
Yongkui Chen Jikuan Qiu Xia-Guang Zhang Huiyong Wang Wenhui Yao Zhiyong Li Qingchun Xia Guangshan Zhu Jianji Wang

Chem Sci 2022 May 27;13(20):5964-5972. Epub 2022 Apr 27.

Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, School of Chemistry and Chemical Engineering, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University Xinxiang Henan 453007 P. R. China

In recent years, covalent organic frameworks (COFs) have attracted enormous interest as a new generation of proton-exchange membranes, chemical sensors and electronic devices. However, to design high proton conductivity COFs, especially those with stimulus responsive performance remains a great challenge. Here, the first example of a light/heat switchable COF (COF-HNU9) has been synthesized by grafting a donor-acceptor Stenhouse adduct (DASA) within the channels of a β-ketoenamine-based COF. DASA groups in the nanopores of COF-HNU9 undergo a reversible open-closed photoisomerization upon visible light irradiation and are recovered by heating. Thus, COF-HNU9 exhibits not only a remarkably high proton conductivity, but also a highly effective switching performance. Under visible light irradiation at 98% RH, the proton conductivity of COF-HNU9 increases by three orders of magnitude at 25 °C and is up to 0.02 S cm at 80 °C. Furthermore, the proton conductivity does not display any significant decrease even after 20 switching cycles. These results have been rationalized by a Grotthuss-type mechanism and verified by DFT calculations. The stimuli-responsive COF is conceptually confirmed by an optical control device with the light/heat switching proton conductive COF-HNU9 film, which is able to remote-control the illumination and switching off of an LED lamp without any current amplifier.
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http://dx.doi.org/10.1039/d2sc02100eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132063PMC
May 2022

Cell-based reporter assays for measurements of antibody-mediated cellular cytotoxicity and phagocytosis against SARS-CoV-2 spike protein.

Authors:
Yuting Hong Huilin Guo Min Wei Yali Zhang Mujin Fang Tong Cheng Zhiyong Li Shengxiang Ge Xiangyang Yao Quan Yuan Ningshao Xia

J Virol Methods 2022 Jun 6;307:114564. Epub 2022 Jun 6.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, PR China; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen, Fujian, PR China.

The COVID-19 pandemic caused by SARS-CoV-2 infections has led to excess deaths worldwide. Neutralizing antibodies (nAbs) against viral spike protein acquired from natural infections or vaccinations contribute to protection against new- and re-infections. Besides neutralization, antibody-mediated cellular cytotoxicity (ADCC) and phagocytosis (ADCP) are also important for viral clearance. However, due to the lack of convenient methods, the ADCC and ADCP responses elicited by viral infections or vaccinations remain to be explored. Here, we developed cell-based assays using target cells stably expressing SARS-CoV-2 spikes and Jurkat-NFAT-CD16a/CD32a effector cells for ADCC/ADCP measurements of monoclonal antibodies and human convalescent COVID-19 plasmas (HCPs). In control samples (n = 190), the specificity was 99.5% (95%CI: 98.4-100%) and 97.4% (95%CI: 95.1-99.6%) for the ADCC and ADCP assays, respectively. Among 87 COVID-19 HCPs, 83 (sensitivity: 95.4%, 95%CI: 91.0-99.8%) and 81 (sensitivity: 93.1%, 95%CI: 87.8-98.4%) showed detectable ADCC (titer range: 7.4-1721.6) and ADCP activities (titer range: 4-523.2). Notably, both ADCC and ADCP antibody titers positively correlated with the nAb titers in HCPs. In summary, we developed new tools for quantitative ADCC and ADCP analysis against SARS-CoV-2, which may facilitate further evaluations of Fc-mediated effector functions in preventing and treating against SARS-CoV-2.
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http://dx.doi.org/10.1016/j.jviromet.2022.114564DOI Listing
June 2022

Downregulation of HINFP induces senescence-associated secretory phenotype to promote metastasis in a non-cell-autonomous manner in bladder cancer.

Authors:
Xianchong Zheng Zefu Liu Jianliang Zhong Liwen Zhou Jiawei Chen Lisi Zheng Zhiyong Li Ruhua Zhang Jingxuan Pan Yuanzhong Wu Zhuowei Liu Tiebang Kang

Oncogene 2022 Jun 6. Epub 2022 Jun 6.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Transcription dysregulation is a salient characteristic of bladder cancer (BC), but no appropriate therapeutic target for it has been established. Here, we found that heterogeneous downregulation of histone H4 transcription factor (HINFP) was associated with senescence in BC tissues and that lower HINFP expression could predict an unfavorable outcome in BC patients. Knockout of HINFP transcriptionally inhibited H1F0 and H1FX to trigger DNA damage, consequently inducing cell senescence to repress the proliferation and growth of BC cells. However, the senescence-associated secretory phenotype, characterized by increases in MMP1/3, enhances the invasion and metastasis of non-senescent BC cells. Histone deacetylase inhibitors (HDACis) could efficiently eliminate the senescent cells induced by HINFP knockout to suppress the invasion and metastasis of BC cells. Our study suggests that HDACis, widely used in multiple cancer types in a clinical context, may also benefit BC patients with metastases induced by cell senescence.
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http://dx.doi.org/10.1038/s41388-022-02371-1DOI Listing
June 2022

Polysaccharide extract from Isatidis Radix inhibits multiple inflammasomes activation and alleviate gouty arthritis.

Authors:
Lutong Ren Qiang Li Hui Li Xiaoyan Zhan Ruichuang Yang Zhiyong Li Zhie Fang Tingting Liu Ziying Wei Jia Zhao Li Lin Wenqing Mou Wenzhang Dai Zhaofang Bai Guang Xu Junling Cao

Phytother Res 2022 Jun 6. Epub 2022 Jun 6.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.

The polysaccharide extract from Isatidis Radix exhibits potent antiinflammatory and antiviral activities, but the mechanism of Isatidis Radix polysaccharide (IRP) remains obscure. Herein, we reported that IRP blocked the activation of nod-like receptor pyrin domain-containing 3 (NLRP3) inflammasome, leading to the inhibiting of caspase-1 cleavage and IL-1β secretion. Mechanistically, IRP did not inhibit NLRP3 inflammasome through suppressing mitochondrial reactive oxygen species (mtROS) production. However, IRP can significantly suppress the oligomerization of apoptosis-associated speck-like protein (ASC) and subsequently block the formation of inflammasome. Next, we evaluate the role of IRP in monosodium urate (MSU)-induced gout in vivo which is a NLRP3-associated disease. We also observed that oral administration of IRP can reduce the increased ankle thickness and the secretion of IL-1β, IL-18, IL-6, TNF-α and MPO of the mouse ankle joints caused by MSU crystals. Furthermore, flow cytometry analysis highlighted a significant modulation of T helper 17 cells (Th17)/regulatory T cells (Treg) following IRP treatment in MSU induced gout. Overall, our findings suggest that IRP has comprehensive and potent antiinflammatory effects and provide a reasonable therapeutic strategy in preventing inflammasome-associated diseases, such as inflammatory gouty arthritis.
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http://dx.doi.org/10.1002/ptr.7514DOI Listing
June 2022

Nkx2.8 promotes chemosensitivity in bladder urothelial carcinoma via transcriptional repression of MDR1.

Authors:
Zhaohui Zhou Longbin Xiong Zeshen Wu Lijuan Jiang Yonghong Li Zhiyong Li Yulu Peng Kang Ning Xiangpeng Zou Zefu Liu Jun Wang Zhen Li Fangjian Zhou Zhuowei Liu Zhiling Zhang Chunping Yu

Cell Death Dis 2022 May 24;13(5):492. Epub 2022 May 24.

Department of Urology, Sun Yat-Sen University Cancer Center, Guangzhou, PR China.

Multidrug resistance gene 1 (MDR1), a key factor contributing to drug insensitivity, has been associated with treatment failure and poor prognoses in various cancers, including bladder urothelial carcinoma (UC). Here we show that positive Nkx2.8 expression was associated with better prognosis of UC patients received chemotherapy. Patients with positive Nkx2.8 expression had promising prognosis from adjuvant chemotherapy. Enforced expression of Nkx2.8 promotes drug sensitivity of UC cells. Mechanistic investigations showed that Nkx2.8 negatively regulated expression of MDR1 by binds directly to the MDR1 promoter and transcriptionally represses MDR1 expression. P-gp inhibitor reversed chemosensitivity inhibition by Nkx2.8 scilencing. In clinical UC specimens, expression of Nkx2.8 inversely correlated with P-gp expression, and UC patients with Nkx2.8 positivity and low P-gp expression displayed the best prognosis. Our findings uncovered a new mechanism of chemosensitivity in UC cells and proposing Nkx2.8-MDR1 axis as a novel candidate target for therapeutic intervention of UC.
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http://dx.doi.org/10.1038/s41419-022-04947-xDOI Listing
May 2022

Effcacy-oriented compatibility for Tianma (), Yanlingcao () and Bingpian () on improving cerebral ischemia stroke by network pharmacology and serum pharmacological methods.

Authors:
L I Zhiyong Zhu Na L I Jianliang Feng Liang Jiang Yanyan L I Caifeng Lin Ling Huang Xiulan

J Tradit Chin Med 2022 06;42(3):408-416

Pharmacological Department, School of Pharmacy, Minzu University of China, Beijing, 100081, China.

Objective: To evaluate the compatibility of Tianma (, TM), Yanlingcao (, YLC) and Bingpian (, BP), and their efficacy in the treatment of cerebral ischemic stroke.

Methods: Network pharmacology was used to determine the compatibility of TM, YLC, and BP, and their potential mechanism. The middle cerebral artery occlusion (MCAO) rat model was used to evaluate the curative effect of the six combinations of TM, YLC, and BP (TZB1-TZB6) on cerebral ischemia, by using the weight matching method to form. The potential component changes of TM and YLC in the blood and brains of rats were analyzed using ultra performance liquid chromatography-mass spectrometry. Finally, molecular docking linked the results of animal experiments and network pharmacology, determining the potential component contributors of TM and YLC to treating ischemic stroke.

Results: TZB reduced the cerebral infarct volume and protected the nerve cells in MCAO rats. The components of TM and YLC were also identified in the blood and brain homogenate, and BP can facilitate the entry of the components of TM and YLC into the blood and brain. Diosgenin, pennogenin, and gastrodin induced effective binding activities with adenosine receptor a1.

Conclusion: We investigate an approach that improves the means of folk prescription combined with multi technology that maybe promote the transformation of Chinese medicinal prescription into component-based Chinese medicine.
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http://dx.doi.org/10.19852/j.cnki.jtcm.2022.03.007DOI Listing
June 2022

Market survey on the traditional medicine of the Lijiang area in Yunnan Province, China.

Authors:
Mingshuo Zhang Haitao Li Junqi Wang Maohong Tang Xiaobo Zhang Shaohua Yang Jianqin Liu Ying Li Xiulan Huang Zhiyong Li Luqi Huang

J Ethnobiol Ethnomed 2022 May 23;18(1):40. Epub 2022 May 23.

China Academy of Chinese Medical Sciences, Beijing, 100700, China.

Background: Traditional markets are important trading places for medicinal plants, and researchers performing market surveys often engage in ethnobotanical research to record the herbal plants used locally and any related traditional knowledge. However, information on market-traded medicinal plants from traditional markets in the Lijiang area of Yunnan is not well documented. This research is an ethnobotanical survey focusing on medicinal plants traded in the traditional markets of the Lijiang area and contributes to the understanding of medicinal plants and related information used by the Naxi people.

Methods: Ethnobotanical surveys were performed for two years (2019-2020). Three traditional markets in the Lijiang area were investigated. The methods we used included literature research, participatory surveys and group discussions. The collected voucher specimens were identified using the botanical taxonomy method and were deposited in the herbarium. The data were analysed through the informant consensus factor and use frequency (UF). These medicinal plants were compared with the Information System of Chinese Rare and Endangered Plants from the Chinese Academy of Sciences. Those results were in turn compared with the Dongba Sutras and Yulong Ben Cao.

Results: A total of 277 species from 97 families were recorded, with Asteraceae providing the maximum numbers of medicinal plants. Among them, 248 species (89%) were wild plants and 266 species (92.39%) were from the local area. Root (40.43%) was the most common medicinal part. A total of 267 species (96.04%) had a UF value above 0.5. Eighty-three investigated human ailments were grouped into 16 categories. Diseases of the digestive system (166 mentions) were most frequently mentioned in this study. There were 19 species of nationally protected plants in China, including 2 species of first-level nationally protected plants and 17 species of second-level nationally protected plants. A total of 31 species of these medicinal plants can be found in the Dongba Sutra or Yulong Ben Cao.

Conclusion: We surveyed the herbal medicine in the markets covering the Lijiang area, analysing and revealing the resource composition and current market situations. The medicinal plants used by the Naxi people are diverse and are used to treat a wide spectrum of body disorders. There are many wild medicinal plants, and to ensure sustainable development, their natural protection should be strengthened. Knowledge of the medicinal plants recorded in Naxi medical classics has ethnobotanical value and should be further developed.
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http://dx.doi.org/10.1186/s13002-022-00532-wDOI Listing
May 2022

Effect of aortic cannulation depth on air emboli transport during cardiopulmonary bypass: A computational study.

Authors:
Raymond Ho Charles McDonald Jo P Pauls Zhiyong Li

Perfusion 2022 May 21:2676591221092942. Epub 2022 May 21.

School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology (QUT), Brisbane, QLD, Australia.

Introduction: Varying the insertion depth of the aortic cannula during cardiopulmonary bypass (CPB) has been investigated as a strategy to mitigate cerebral emboli, yet its effectiveness associated with CPB flow is not fully understood. We compared different arterial cannula insertion depths and pump flow influencing air microemboli entering the aortic arch branch arteries (AABA).

Methods: A computational approach used a patient-specific aorta model to evaluate four cannula locations at (1) proximal arch, (2) mid arch, (3) distal arch, and (4) descending aorta. We injected 0.1 mm microemboli (N=720) at 2 and 5 L/min and assessed the embolic load and the particle averaged transit times ( entering the AABA.

Results: Location 4 had the lowest embolic load (2 L/min: N= 63) and (5 L/min: N= 54) compared to locations 1 to 3 in the range of (N= 118 to 116 at 2 L/min:) and (N= 92 to 146 at 5 L/min). There was no significant difference between 2 L/min and 5 L/min (p = 0.31), despite 5 L/min attaining a lower mean (±standard deviation) than 2 L/min (38.0±23.4 vs 44.5±21.1), respectively. Progressing from location 1 to 4, increased 3.11s -7.40 s at 2 L/min and 1.81s -4.18s at 5 L/min.

Conclusion: It was demonstrated that the elongated cannula insertion length resulted in lower embolic loads, particularly at a higher flow rate. The numerical results suggest that CPB management could combine active flow variation with improving cannula performance and provide a foundation for a future experimental and clinical investigation to reduce surgical cerebral air microemboli.
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http://dx.doi.org/10.1177/02676591221092942DOI Listing
May 2022

essential oil attenuates acetaminophen-induced liver injury through alleviating oxidative stress and activating autophagy.

Authors:
Jing Zhao Kaixin Ding Manting Hou Yuanhua Li Xiaorong Hou Wenzhang Dai Zhiyong Li Jun Zhao Wenlong Liu Zhaofang Bai

Pharm Biol 2022 Dec;60(1):958-967

Department of Hepatology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.

Context: (Turcz.) Baill. (Magnoliaceae) essential oil (SCEO) composition is rich in lignans that are believed to perform protective effects in the liver.

Objective: This study investigates the effects of SCEO in the treatment of acetaminophen (APAP)-induced liver injury in mice.

Materials And Methods: C57BL/6 mice ( = 56) were randomly divided into seven groups: normal; APAP (300 mg/kg); APAP plus bicyclol (200 mg/kg); APAP plus SCEO (0.25, 0.5, 1, 2 g/kg). Serum biochemical parameters for liver function, inflammatory factors, and antioxidant activities were determined. The protein expression levels of Nrf2, GCLC, GCLM, HO-1, p62, and LC3 were assessed by western blotting. Nrf2, GCLC, HO-1, p62, and LC3 mRNA were detected by real-time PCR.

Results: Compared to APAP overdose, SCEO (2 g/kg) pre-treatment reduced the serum levels of AST (79.4%), ALT (84.6%), TNF-α (57.3%), and IL-6 (53.0%). In addition, SCEO (2 g/kg) markedly suppressed cytochrome P450 2E1 (CYP2E1) (15.4%) and attenuated the exhaustion of GSH (43.6%) and SOD (16.8%), and the accumulation of MDA (22.6%) in the liver, to inhibit the occurrence of oxidative stress. Moreover, hepatic tissues from our experiment revealed that SCEO pre-treatment mitigated liver injury caused by oxidative stress by increasing Nrf2, HO-1, and GCL. Additionally, SCEO activated autophagy, which upregulated hepatic LC3-II and decreased p62 in APAP overdose mice ( < 0.05).

Discussion And Conclusions: Our evidence demonstrated that SCEO protects hepatocytes from APAP-induced liver injury and the findings will provide a reliable theoretical basis for developing novel therapeutics.
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http://dx.doi.org/10.1080/13880209.2022.2067569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122381PMC
December 2022

Virome analysis of ticks in Zhoushan Archipelago, China.

Authors:
Ting He Changqiang Zhu Zhiyong Li Lele Ai Dan Hu Chunhui Wang Feng Li Xiaohong Yang Heng Lv Wangeng Chen Hui Qian Weilong Tan Changjun Wang

J Vet Med Sci 2022 Jun 17;84(6):847-854. Epub 2022 May 17.

Chinese People's Liberation Army Center for Disease Control and Prevention, Beijing, China.

Ticks are an important group of arthropod vectors. Ticks pose a profound risk to public health by transmitting many types of microorganisms that are human and animal pathogens. With the development of next-generation sequencing (NGS) technology and viral metagenomics, numerous novel viruses have been discovered in ticks and tick-related hosts. To fully understand the virus spectrum in ticks in the Zhoushan Archipelago of Zhejiang province in China, ticks were collected from Qushan Island, Zhoushan Island, and Daishan Island in the Zhoushan Archipelago in June 2016. NGS performed to investigate the diversity of tick-associated viruses identified 21 viral sequences. Twelve were pathogenic to humans and animals. Trough verification by polymerase chain reaction (PCR) revealed the existence of three tick-associated viruses with extensive homology with Dabieshan, MG22, and Odaw virus. Other NGS-detected sequences that could not be amplified by PCR were highly homologous (92-100%) with known pathogenic viruses that included hepatitis B virus, papillomavirus, and human mastadenovirus C. This is the first study to systematically apply high throughput sequencing technology to explore the spectrum of viruses carried by ticks in the Zhoushan Archipelago. The findings are fundamental knowledge of the diversity of tick-associated viruses in this region and will inform strategies to monitor and prevent the spread of tick-borne diseases.
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http://dx.doi.org/10.1292/jvms.22-0058DOI Listing
June 2022

Complete genome of Mycetocola spongiae MSC19 isolated from deep-sea sponge Cacospongia mycofijiensis indicates the adaptation to deep-sea environment and sponge-microbe symbioses.

Authors:
Yuling Chen Tianjiao Pan Guangjun Chai Zhiyong Li

Mar Genomics 2022 Jun 6;63:100955. Epub 2022 May 6.

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

Genome of Mycetocola spongiae MSC19, a novel marine sponge-associated Actinobacteria isolated from the Mariana Trench sponge Cacospongia mycofijiensis, was sequenced. The genome has one circular chromosome of 3,196,754 bp, with an average GC content of 66.43 mol%, and 2887 coding sequences. Gene annotation shows that M. spongiae MSC19 possesses series of genes related to adaptation to deep-sea environmental stresses including cold shock, heat shock, osmotic stress and oxidative stress. Genes encoding for heavy metal resistance, multidrug resistance and multiple natural product biosynthesis which are crucial for survival in the extreme environment are also detected in the genome. The potentials to synthesize kinds of vitamins and eukaryotic-like proteins indicates the possible nutrient exchange and mutual recognization between M. spongiae MSC19 and its sponge host. The genome provides insights into the stress resistance and ecological fitness of bacterial symbionts in the deep-sea sponge holobionts.
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http://dx.doi.org/10.1016/j.margen.2022.100955DOI Listing
June 2022

bZIP71 delays flowering by suppressing Ehd1 expression in rice.

Authors:
Xiufeng Li Xiaojie Tian Mingliang He Xinxin Liu Zhiyong Li Jiaqi Tang Enyang Mei Min Xu Yingxiang Liu Zhenyu Wang Qingjie Guan Wei Meng Jun Fang Jian Zhang Qingyun Bu

J Integr Plant Biol 2022 May 11. Epub 2022 May 11.

Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, the Chinese Academy of Sciences, Harbin, 150081, China.

Flowering time is a fundamental factor determining the global distribution and final yield of rice (Oryza sativa). Although diverse flowering time genes have been reported in this crop, the transcriptional regulation of its key flowering genes are poorly understood. Here, we report that a basic leucine zipper transcription factor, bZIP71, functions as a flowering repressor. The overexpression of bZIP71 delays flowering, while the bzip71 mutant flowers early in both long-day and short-day conditions. A genetic analysis showed that the regulation of flowering by bZIP71 might be independent of Heading date 2 (Hd2), Hd4, and Hd5. Importantly, bZIP71 directly associates with the Early heading date 1 (Ehd1) promoter and represses its transcription, and genetically the function of bZIP71 is impaired in the ehd1 mutant. Moreover, bZIP71 interacts with major components of polycomb repressive complex 2 (PRC2), SET domain group protein 711 (SDG711), and Fertilization independent endosperm 2 (FIE2), through which bZIP71 regulates the H3K27me3 level of Ehd1. Taken together, we present a transcriptional regulatory mechanism in which bZIP71 enhances the H3K27me3 level of Ehd1 and transcriptionally represses its expression, which not only offers a novel insight into a flowering pathway, but also provides a valuable putative target for the genetic engineering and breeding of elite rice cultivars.
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http://dx.doi.org/10.1111/jipb.13275DOI Listing
May 2022

Co-intercalation strategy of constructing partial cation substitution of ammonium vanadate {(NH)VO} for stable zinc ion storage.

Authors:
Rui Sun Siyang Dong Feng Xu Zhiyong Li Caihong Wang Shengjun Lu Haosen Fan

Dalton Trans 2022 May 17;51(19):7607-7612. Epub 2022 May 17.

School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, China.

Recently, aqueous zinc-ion batteries have become a hot research topic in the field of grid-scale application, which can be attributed to their low-cost, aqueous electrolyte and dominant theoretical reversible capacity. Nevertheless, the lack of suitable cathode materials greatly hinders the development of aqueous zinc-ion batteries. In this work, we adopt a simple one-step synthesis strategy to prepare (NH)VO with an intercalation of Na and HO, which exhibits a novel crystal structure in which the ammonium ion, crystal water, and sodium ion co-locate in the VO layers. The co-intercalation not only effectively enhances the binding energy between V-O layers to suppress vanadium dissolution but also successfully improves the structural stability to alleviate the structural collapse during the cyclic process. As result, (NH)VO with the intercalation of crystal water and Na presents a remarkable reversible discharge capacity of 423.9 mA h g after 90 cycles at 0.1 A g with an excellent energy density of 350.3 W h kg and demonstrates an outstanding specific capacity of 182.5 mA h g at the high current density of 5 A g upon 1400 cycles during the ultra-wide voltage window of 0.1-2.0 V.
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http://dx.doi.org/10.1039/d2dt00665kDOI Listing
May 2022

Identification of serum metabolites enhancing inflammatory responses in COVID-19.

Authors:
Chen-Song Zhang Bingchang Zhang Mengqi Li Xiaoyan Wei Kai Gong Zhiyong Li Xiangyang Yao Jianfeng Wu Cixiong Zhang Mingxia Zhu Lei Zhang Xiufeng Sun Yi-Hong Zhan Zhengye Jiang Wenpeng Zhao Wei Zhong Xinguo Zhuang Dawang Zhou Hai-Long Piao Sheng-Cai Lin Zhanxiang Wang

Sci China Life Sci 2022 Apr 28. Epub 2022 Apr 28.

Department of Neurosurgery, Xiamen Key Laboratory of Brain Center, the First Affiliated Hospital of Xiamen University, Xiamen, 361102, China.

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is characterized by a strong production of inflammatory cytokines such as TNF and IL-6, which underlie the severity of the disease. However, the molecular mechanisms responsible for such a strong immune response remains unclear. Here, utilizing targeted tandem mass spectrometry to analyze serum metabolome and lipidome in COVID-19 patients at different temporal stages, we identified that 611 metabolites (of 1,039) were significantly altered in COVID-19 patients. Among them, two metabolites, agmatine and putrescine, were prominently elevated in the serum of patients; and 2-quinolinecarboxylate was changed in a biphasic manner, elevated during early COVID-19 infection but levelled off. When tested in mouse embryonic fibroblasts (MEFs) and macrophages, these 3 metabolites were found to activate the NF-κB pathway that plays a pivotal role in governing cytokine production. Importantly, these metabolites were each able to cause strong increase of TNF and IL-6 levels when administered to wildtype mice, but not in the mice lacking NF-κB. Intriguingly, these metabolites have little effects on the activation of interferon regulatory factors (IRFs) for the production of type I interferons (IFNs) for antiviral defenses. These data suggest that circulating metabolites resulting from COVID-19 infection may act as effectors to elicit the peculiar systemic inflammatory responses, exhibiting severely strong proinflammatory cytokine production with limited induction of the interferons. Our study may provide a rationale for development of drugs to alleviate inflammation in COVID-19 patients.
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http://dx.doi.org/10.1007/s11427-021-2099-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068507PMC
April 2022

Role of Sam68 in Sunitinib induced renal cell carcinoma apoptosis.

Authors:
Zeshen Wu Yulu Peng Longbin Xiong Jun Wang Zhen Li Kang Ning Minhua Deng Ning Wang Wensu Wei Zhiyong Li Pei Dong Chunping Yu Fangjian Zhou Zhiling Zhang

Cancer Med 2022 Apr 10. Epub 2022 Apr 10.

Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, China.

Sunitinib is one of the first-line targeted drugs for metastatic renal cell carcinoma (RCC) with dual effects of antiangiogensis and proapoptosis. Sam68 (Src-associated in mitosis, 68 KDa), is found being involved in cell apoptosis. This article reveals that Sam68 impacts the sensitivity to sunitinib by mediating the apoptosis of RCC cells. Immunohistochemical staining indicated that the Sam68 expression levels in sunitinib sensitive tumor tissues were markedly higher than those in sunitinib resistant tumor tissues. Sunitinib induced RCC cell apoptosis in a concentration-dependent manner and inhibited the expression of total and phosphorylated Sam68 (p-Sam68). Downregulation of Sam68 expression inhibited RCC cell apoptosis induced by sunitinib. While upregulation of Sam68 expression could enhance apoptosis induced by sunitinib. Xenograft models showed that tumors in the Sam68-knockdown group did not shrink as much as those in the control group after treatment with sunitinib for 4 weeks. Together, our results suggest that Sam68 expression is associated with the sensitivity of ccRCC patients to sunitinib. Sam68 may promote cell apoptosis induced by sunitinib, and the Sam68 expression level may be a biomarker for predicting sunitinib sensitivity in ccRCC patients.
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http://dx.doi.org/10.1002/cam4.4743DOI Listing
April 2022

TAU ablation in excitatory neurons and postnatal TAU knockdown reduce epilepsy, SUDEP, and autism behaviors in a Dravet syndrome model.

Authors:
Eric Shao Che-Wei Chang Zhiyong Li Xinxing Yu Kaitlyn Ho Michelle Zhang Xin Wang Jeffrey Simms Iris Lo Jessica Speckart Julia Holtzman Gui-Qiu Yu Erik D Roberson Lennart Mucke

Sci Transl Med 2022 04 27;14(642):eabm5527. Epub 2022 Apr 27.

Gladstone Institute of Neurological Disease, Gladstone Institutes, San Francisco, CA 94158, USA.

Intracellular accumulation of TAU aggregates is a hallmark of several neurodegenerative diseases. However, global genetic reduction of TAU is beneficial also in models of other brain disorders that lack such TAU pathology, suggesting a pathogenic role of nonaggregated TAU. Here, conditional ablation of TAU in excitatory, but not inhibitory, neurons reduced epilepsy, sudden unexpected death in epilepsy, overactivation of the phosphoinositide 3-kinase-AKT-mammalian target of rapamycin pathway, brain overgrowth (megalencephaly), and autism-like behaviors in a mouse model of Dravet syndrome, a severe epileptic encephalopathy of early childhood. Furthermore, treatment with a TAU-lowering antisense oligonucleotide, initiated on postnatal day 10, had similar therapeutic effects in this mouse model. Our findings suggest that excitatory neurons are the critical cell type in which TAU has to be reduced to counteract brain dysfunctions associated with Dravet syndrome and that overall cerebral TAU reduction could have similar benefits, even when initiated postnatally.
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http://dx.doi.org/10.1126/scitranslmed.abm5527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102397PMC
April 2022

Editorial: Computational Biomechanics of the Heart and Vasculature With Potential Clinical and Surgical Applications.

Authors:
Zhiyong Li Youjun Liu Estefania Peña Daniela Valdez-Jasso Dalin Tang

Front Physiol 2022 7;13:872774. Epub 2022 Apr 7.

Mathematical Sciences Department, Worcester Polytechnic Institute, Worcester, MA, United States.

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http://dx.doi.org/10.3389/fphys.2022.872774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022173PMC
April 2022

Classification of unilateral thalamic gliomas predicts tumor resection and patient's survival: a single center retrospective study.

Authors:
Tianshi Que Zhiyong Li Haojie Zheng Jian-Er Tan Xi Yuan Guozhong Yi Luxiong Fang Jing Nie Yanyi Yin Haiyan Xu Xiaoxuan Zheng Junlu Liu Xi-An Zhang Songtao Qi Guanglong Huang

J Neurosurg Sci 2022 Apr 13. Epub 2022 Apr 13.

Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.

Background: To propose our classification about unilateral thalamic gliomas, and to describe relationship between the classification and clinical characteristics including symptoms, surgical approaches and survival, which should contribute to the treatment and the prognostic prediction of unilateral thalamic gliomas.

Methods: A total of 66 adult unilateral thalamic glioma patients with pathologic confirmation between January 2010 and December 2018 were retrospectively investigated.

Results: Unilateral thalamic gliomas could be divided into quadrigeminal cistern and ventricle extension type (Type Q), lateral type (Type L) and anterior type (Type A) according to tumor location, extensive polarity and location of ipsilateral posterior limb of internal capsule. Each subtype of QLA classification could match with one kind of corresponding approach. Preoperative symptoms including headache, dyskinesia, aphasia, hydrocephalus and KPS scores, and pathological features including H3K27M mutation and P53 expression were correlated with QLA classification. Further analysis confirmed that Type Q tumors had a higher rate of total resection and a significantly longer survival time compared to Type L and Type A tumors, with similar improved and deteriorated rates of symptoms. Univariate and multivariate analysis demonstrated QLA classification was remarkedly associated with overall survival and could be considered as an independent prognostic factor in patients with unilateral thalamic gliomas.

Conclusions: Unilateral thalamic glioma could be divided into 3 subtypes by imaging characteristics, symptoms and survival. QLA classification could predict tumor resection and the prognosis and could contribute to the planning of therapeutic strategy in patients with unilateral thalamic gliomas.
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http://dx.doi.org/10.23736/S0390-5616.22.05660-0DOI Listing
April 2022

Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma.

Authors:
Zhiying Lin Zhu Zhang Haojie Zheng Haiyan Xu Yajuan Wang Chao Chen Junlu Liu Guozhong Yi Zhiyong Li Xiaoyan Wang Guanglong Huang

Cancer Cell Int 2022 Apr 11;22(1):151. Epub 2022 Apr 11.

Department of Neurosurgery, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Avenue North, Guangzhou, 510515, Guangdong, China.

Background: Compared with the proneural (PN) subtype of glioblastoma (GBM), the mesenchymal (MES) subtype is more invasive and immune evasive and is closely related to poor prognosis. Here, we used transcriptome data and experimental evidence to indicate that CUB domain-containing protein 1 (CDCP1) is a novel regulator that facilitates the transformation of PN-GBM to MES-GBM.

Methods: The mRNA expression data of CDCP1 in glioma were collected from the TCGA, CGGA and GEO databases, and in vitro experiments verified CDCP1 expression in glioma tissue samples. Independent prognostic analysis revealed the correlation of the CDCP1 expression level and patient survival. Bioinformatics analysis and experiments verified the biological function of CDCP1. Multivariate proportional hazards models and a PPI network were used to select key genes. A prognostic risk model for predicting the survival of glioma patients was constructed based on the selected genes.

Results: The results showed that the expression of CDCP1 increased with increasing tumor grade and that the overexpression of CDCP1 correlated with a poor prognosis. CDCP1 was highly expressed in MES-GBM but weakly expressed in PN-GBM. The risk model (considering CDCP1 combined with CD44 and ITGAM expression) could represent a tool for predicting survival and prognosis in glioma patients.

Conclusions: Our study indicates that CDCP1 plays an important role in facilitating the transformation of PN-GBM to MES-GBM.
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http://dx.doi.org/10.1186/s12935-021-02373-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9003964PMC
April 2022

Characterizing and Predicting the Resilient Modulus of Recycled Aggregates from Building Demolition Waste with Breakage-Induced Gradation Variation.

Authors:
Yuanjie Xiao Kunfeng Kong Umar Faruk Aminu Zhiyong Li Qiang Li Hongwei Zhu Degou Cai

Materials (Basel) 2022 Apr 5;15(7). Epub 2022 Apr 5.

State Key Laboratory for High-Speed Railway Track Technology, China Academy of Railway Sciences Corporation Limited, Beijing 100081, China.

Building demolition waste (BDW) has been massively stockpiled due to increasingly rapid urbanization and modernization. The use of recycled BDW as unbound granular base/subbase materials is among the sustainable, cost-effective, and environmentally friendly pavement construction alternatives. The resilient modulus is an important mechanical property of BDW-derived aggregates and mechanistic design input of pavements incorporating BDW. This paper presents the results of a comprehensive laboratory study on the shear strength and resilient modulus characteristics of BDW-derived aggregate materials. A series of monotonic triaxial compression tests and repeated-load triaxial (RLT) tests were conducted with five different gradations representing particle breakage and different stress paths. The apparent cohesion and internal friction angle of recycled BDW aggregates under consolidated drained conditions ranged from 35.3 to 57.5 kPa and from 30.2° to 54.3°, respectively. The apparent cohesion and internal friction angle also increased and decreased non-linearly with the increasing relative content of fine particles, respectively. The resilient modulus of recycled BDW aggregates gradually decreased with increasing relative content of fine particles at the same stress level. Both the deviator stress and confining pressure exhibited significant influences on the resilient modulus, while the effect of confining pressure was more profound. Based on laboratory testing data, a mechanistic-empirical model was developed to predict the resilient modulus of recycled BDW aggregates from gradation and stress-state variables. The findings could be useful for extended engineering applications of BDW in unbound granular pavement base/subbase construction.
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http://dx.doi.org/10.3390/ma15072670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000266PMC
April 2022

Hole Morphology and Keyhole Evolution during Single Pulse Laser Drilling on Polyether-Ether-Ketone (PEEK).

Authors:
Yanmei Zhang Gang Yu Chongxin Tian Zhiyong Li Jiayun Shao Shaoxia Li Xiuli He

Materials (Basel) 2022 Mar 26;15(7). Epub 2022 Mar 26.

Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China.

Polyether-ether-ketone (PEEK), with its superior mechanical, chemical, and thermal properties, as well as high biocompatibility, has been used in aerospace, electronics, and biomedical applications. In this paper, a large number of experiments of single-pulse laser drilling on PEEK were performed to analyze the hole morphology and keyhole evolution, which were characterized by an optical microscope, charge-coupled device (CCD), and high-speed camera. A novel method is proposed to observe and measure the dimension of the processed hole rapidly right after laser drilling for special polymer materials with wear-resistance and non-conductivity. Morphological characteristics of holes are presented to illustrate the effect of pulse width and peak power on hole depth, hole diameter, and aspect-ratio. The obtained maximum drilling depth was 7.06 mm, and the maximum aspect-ratio was 23. In situ observations of the dynamic process of laser drilling, including the keyhole evolution together with ejection and vaporization behavior, were also carried out. The keyhole evolution process can be divided into three stages: rapid increment stage (0-2 ms) at a rate of 2.1 m/s, slow increment stage (2-4 ms) at a rate of 0.3 m/s, and stable stage (>4 ms). Moreover, the variation of dimensionless laser power density with the increase in pulse width was calculated. The calculated maximum drilling depth based on energy balance was compared with the experimental depth. It is proven that the laser-PEEK interaction is mainly influenced by a photothermal effect. Ejection is the dominant material-removal mechanism and contributes to over 60% of the depth increment during the rapid increment stage, while vaporization is dominant and contributes to about 80% of the depth increment during the slow increment stage. The results reveal the material removal mechanism for single-pulse laser drilling on PEEK, which is helpful to understand the dynamic process of keyhole evolution. This not only provides a processing window for future laser drilling of PEEK but also gives a guide for the manufacturing of other polymers.
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http://dx.doi.org/10.3390/ma15072457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999681PMC
March 2022

Integration of Transcriptomic and Metabolomic Data to Compare the Hepatotoxicity of Neonatal and Adult Mice Exposed to Aristolochic Acid I.

Authors:
Zhi-E Fang Chunyu Wang Ming Niu Tingting Liu Lutong Ren Qiang Li Zhiyong Li Ziying Wei Li Lin Wenqing Mu Yuan Gao Xiaohe Xiao Zhaofang Bai

Front Genet 2022 25;13:840961. Epub 2022 Mar 25.

Department of Hepatology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.

Aristolochic acid (AA) is a group of structurally related compounds what have been used to treat various diseases in recent decades. Aristolochic acid I (AAI), an important ingredient, has been associated with tumorigenesis. Recently, some studies indicated that AAI could induce liver injury in mice of different age, but comprehensive mechanisms of AAI-induced differences in liver injury in various age groups have not yet been elucidated. This study aims to evaluate the causal relationship between AAI-induced liver injury and age based on neonatal mice and adult mice. A survival experiment indicated that all neonatal mice survived. Moreover, the adult mice in the high-dose AAI group all died, whereas half of the adult mice in the low-dose AAI group died. In observation experiments, AAI induced more severe liver injury in neonatal mice than adult mice under long-term than short-term exposure. Furthermore, integrated metabolomics and transcriptomics indicated that AAI disturbing steroid hormone biosynthesis, arachidonic acid metabolism, the drug metabolism-cytochrome P450 pathway and glycerophospholipid metabolism induced neonatal mice liver injury. The important role of age in AAI-induced liver injury was illustrated in our study. This study also lays a solid foundation for scientific supervision of AA safety.
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http://dx.doi.org/10.3389/fgene.2022.840961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8992794PMC
March 2022

Randomized Clinical Trial: Probiotics Alleviated Oral-Gut Microbiota Dysbiosis and Thyroid Hormone Withdrawal-Related Complications in Thyroid Cancer Patients Before Radioiodine Therapy Following Thyroidectomy.

Authors:
Baiqiang Lin Fuya Zhao Yang Liu Xin Wu Jing Feng Xiangren Jin Wei Yan Xiao Guo Shang Shi Zhiyong Li Lujia Liu Hongye Chen Haoran Wang Shuang Wang Yu Lu Yunwei Wei

Front Endocrinol (Lausanne) 2022 8;13:834674. Epub 2022 Mar 8.

Department of Oncology and Laparoscopy Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Background: Thyroid hormone withdrawal (THW) in postoperative thyroid cancer patients who need always accompanied by complications (e.g., dyslipidemia and constipation). At present, there are no effective and safe means to alleviate these complications.

Purpose: We aimed to assess the oral-gut microbiota profiles in THW patients then investigate whether probiotics could alleviating alleviate THW related complications and investigate whether these therapeutic effects were associated with the oral-gut microbiota state.

Methods: Fifty eligible thyroid carcinoma patients undergoing thyroidectomy were randomly assigned to receive probiotics or placebo during THW. Complications were assessed through validated questionnaires and plasma lipid indicators. The complex probiotics preparation was composed of , , , and .

Results: Probiotics alleviated lack of energy, constipation, weight gain, and dry mouth and decreased the levels of fecal/serum LPS and plasma lipid indicators (total cholesterol, triglycerides, low-density lipoprotein, and apolipoprotein A) (P < 0.05). Gut and oral microbial diversity were significantly decreased after THW, while an increased microbial dysbiosis index (MDI) was observed. Probiotics distinctly restored the gut and oral microbial diversity. Increased , , and , while decreased , , , and in the gut were found after probiotics intervention. Lack of energy, constipation, weight gain, and dyslipidemia were seen to be related to the above microbiota. In addition, probiotics reduced oral , , , and , which were positively correlated with the occurrence of dry mouth.

Conclusion: Probiotics reduce the incidence of complications in patients after THW, which may be related to modifying the oral and gut microbiota.

Clinical Trial Registration: [https://clinicaltrials.gov/], identifier America Clinical Trial Registry NCT03574051.
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http://dx.doi.org/10.3389/fendo.2022.834674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8958007PMC
April 2022

sp. nov., isolated from deep-sea sponge .

Authors:
Yuling Chen Jin Sang Wei Sun Qianqian Song Zhiyong Li

Int J Syst Evol Microbiol 2022 Mar;72(3)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.

A novel bacterial strain (MSC19) was isolated from a deep-sea sponge collected in the Mariana Trench at a depth of 2681 m. The cells of the new isolate were Gram-stain-positive, non-motile, oxidase- and catalase-positive, rod-shaped and yellow-coloured. They could grow at 4-32 °C (optimum, 28 °C), pH 5.5-12 (optimum, pH 7.0) and with 0-12 % (w/v) NaCl (optimum, 4 %). The strain's 16S rRNA gene sequence showed 98.41 % similarity to that of CM-01. Phylogenetic analysis further suggested that strain MSC19 represents a new species within the genus . The total genome of MSC19 was approximately 3 196 754 bp in size with a G+C content of 66.43 mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among MSC19 and other type strains were 70.35-75.37 % (ANIb), 83.79-84.73 % (ANIm) and 20.3-21.7 % (dDDH). The major fatty acids of MSC19 were composed of anteiso-C, iso-C and anteiso-C, and its predominant menaquinones were MK-10 and MK-9. The polar lipids of MSC19 mainly consisted of diphosphatidylglycerol, phosphatidylglycerol and glycolipid. The diagnostic cell-wall diamino acid was lysine. Combined molecular, physiological, biochemical and chemotaxonomic analyses suggest that strain MSC19 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MSC19 (=MCCC 1K06265=KCTC 49701).
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http://dx.doi.org/10.1099/ijsem.0.005291DOI Listing
March 2022
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