Publications by authors named "Zhixiang Xu"

101 Publications

A molecularly-imprinted SERS sensor based on a [email protected] substrate for the selective capture and sensitive detection of tryptamine in foods.

Food Chem 2022 Nov 21;394:133536. Epub 2022 Jun 21.

Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, People's Republic of China. Electronic address:

Herein, a molecularly imprinted surface-enhanced Raman spectroscopy (SERS) sensor was developed for the selective capture and sensitive detection of tryptamine in foods. The SERS sensor exploited silver nanoparticle-decorated TiO ([email protected]) substrates for Raman signal enhancement via synergistic effect of electromagnetic enhancement and photoinduced charge-transfer, whilst surface functionalization with the molecularly imprinted polymer ensured selective tryptamine capture. The SERS spectrum of tryptamine on the sensor closely matched that predicted by density functional simulations. The SERS intensity for tryptamine on the developed [email protected]@MIP sensor increased linearly with the logarithm of the tryptamine concentration over the range of 10-10 mol L, with a LOD of 4.85 × 10 mol L. Tryptamine was detected in a spiked white vinegar sample, and its recoveries were in the range of 92.00%-111.40%. The SERS sensor could be used for the detection of tryptamine in actual samples.
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http://dx.doi.org/10.1016/j.foodchem.2022.133536DOI Listing
November 2022

Ultra-sensitive detection of streptomycin in foods using a novel SERS switch sensor fabricated by AuNRs array and DNA hydrogel embedded with DNAzyme.

Food Chem 2022 Nov 7;393:133413. Epub 2022 Jun 7.

Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, People's Republic of China. Electronic address:

Detrimental health effects caused by the intake of food contaminated with streptomycin have drawn concerns on effective monitoring using sensitive and selective methods. In this work, a DNA hydrogel surface enhanced Raman spectroscopy (SERS) sensor was successfully developed for the ultrasensitive determination of streptomycin residues in foods. The sensor used a DNA hydrogel containing DNAzyme (Pb-DNAzyme), triggering release of the Raman reporter 4-mercaptobenzonitrile, which was detected using a gold nanorods (AuNRs) array. The linear range of the sensor was 0.01-150 nM and the limit of detection was 4.85 × 10 nM. Tests conducted with four streptomycin structural analogues confirmed the sensor was specific. Milk and honey samples spiked with streptomycin were analysed, resulting in standard recoveries in the range 98.2-117.3%. These findings demonstrated that such a sensor can be used for ultrasensitive detection of streptomycin in foods.
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http://dx.doi.org/10.1016/j.foodchem.2022.133413DOI Listing
November 2022

Investigation of Synergistic Effects and Kinetics on Co-Pyrolysis of and Waste Tires.

Int J Environ Res Public Health 2022 06 9;19(12). Epub 2022 Jun 9.

Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education, School of Energy and Environment, Southeast University, Nanjing 210096, China.

A thermogravimetric analysis is used to analyze the thermal kinetics and investigate the synergistic effects between (AP) and waste tires (WTS) in a temperature range of 50-900 °C under three heating rates (15, 25, and 35 °C/min). Two model-free methods (FWO and KAS) and a model-fitting method (CR) were applied to calculate the activation energy. Results revealed that heating rates had no significant effect on the pyrolysis operation. The addition of WTS improved the thermal degradation of the samples as the samples had more than one stage during the main reaction period. A promoting synergistic effect was found in the blend 75A25WT and obtained the lowest activation energy among all the blends without a catalyst, while the blend 50A50WT exhibited an inhibiting effect. On the other hand, the addition of HZSM-5 accelerated the reaction time and obtained the lowest activation energy among all the blends without a catalyst. Furthermore, ΔW of 75A25WT+C was the lowest, indicating that the blend with a catalyst exhibited the strongest synergistic effect. This research confirmed that the addition of WTS improved the thermal parameters of the samples and clarified the capacity of HZSM-5 to reduce the activation energy.
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http://dx.doi.org/10.3390/ijerph19127101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222625PMC
June 2022

Synthesis of biotinylated-LPG as a chemical biology tool enabling discovery of ALCAT1 modulators.

Bioorg Med Chem Lett 2022 09 16;72:128861. Epub 2022 Jun 16.

Structural & Protein Sciences, Janssen Research & Development, Welsh and McKean Roads, Spring House, PA 19477-0776, United States.

As a mitochondrial signature phospholipid, cardiolipin (CL) is required for membrane structure, respiration, dynamics, fragmentation, and mitophagy. Alteration of CL by reactive oxygen species (ROS) can cause mitochondrial dysfunction, which is implicated in the pathogenesis of many diseases. The enzyme ALCAT1 (acyl-CoA: lysocardiolipin acyltransferase-1) facilitates the conversion of CL by incorporating polyunsaturated fatty acids into lysocardiolipin. Accumulating evidence suggests that overexpression of ALCAT1 is involved in pathological cardiolipin remodeling and mitochondrial bioenergetics. Few ALCAT1 modulators are reported in the literature, and the enzymatic activity was tested via a low-throughput TLC (thin layer chromatography) assay. To identify small molecule ALCAT1 inhibitors, a robust assay was needed to enable a full deck high throughput screen. Scintillation proximity assay (SPA) was the method of choice because it permits the rapid and sensitive measurement of a broad range of biological processes in a homogeneous system. A biotinylated ALCAT1 substrate was required as a chemical biology tool in developing SPA. Among a panel of phospholipids, lysophosphatidyl glycerol (LPG) was identified as the best substrate for ALCAT1. Herein we report the synthesis of biotinylated-LPG analogs with varied linker lengths and their activity towards ALCAT1.
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http://dx.doi.org/10.1016/j.bmcl.2022.128861DOI Listing
September 2022

Enhancing production of hydrocarbon-rich bio-oil from biomass via catalytic fast pyrolysis coupled with advanced oxidation process pretreatment.

Bioresour Technol 2022 Sep 10;359:127450. Epub 2022 Jun 10.

Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education, Southeast University, No.2 Sipailou, Xuanwu District, Nanjing, Jiangsu 210096, China.

This study aims to propose a method for upgrading biomass pyrolysis products based on the combination of sodium persulfate pretreatment and fast catalytic pyrolysis. Combined with the analysis of components and thermogravimetric analysis, the result showed that after pretreatment the biomass structure was gradually depolymerized, the contents of lignin, the reaction of activation energy and the crystallinity of cellulose decreased. Due to the destructive effect of persulfate radicals, in fast pyrolysis, the relative contents of acids and oxygen-containing substances decreased, and the relative content of phenols can significantly increase to 19.20%. The yield of aromatic hydrocarbons and total hydrocarbons had a high value under the catalytic pyrolysis in the best performance which amount of yield reached 28.66% and 33.72%, respectively. Sodium persulfate pretreatment was beneficial in the production of hydrocarbon-rich bio-oils and high-value chemicals since the radicals can effectively depolymerize lignin which promoted the process of pyrolysis.
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http://dx.doi.org/10.1016/j.biortech.2022.127450DOI Listing
September 2022

Preparation of Magnetic Metal-Organic [email protected] Imprinted Nanoparticles for Specific Extraction and Enrichment of Bisphenol A in Food.

Foods 2022 May 12;11(10). Epub 2022 May 12.

Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.

Metal-organic frameworks (MOFs) with systematically tailored structures have been suggested as promising precursors to the preparation of diverse functional materials. Herein, a facile and versatile layer-by-layer strategy without any special surface modifications has been proposed for the preparation of magnetic metal-organic frameworks (MMOFs) supported molecularly imprinted polymer nanoparticles ([email protected]), which are based on a magnetically susceptible core conjugated with an imidazole-derived self-assembled layer and a silane-based imprinted shell. The obtained [email protected], which integrated the advantages of FeO, MOFs, and MIPs, were characterized and exhibited good magnetic properties, a rapid mass transfer rate, and an excellent adsorption selectivity as well as capacity for the targeted molecular - bisphenol A (BPA). Moreover, the [email protected] were employed as adsorbents in magnetic solid phase extraction (MSPE) to selectively bind and rapidly separate BPA from real samples with satisfactory recoveries ranging from 88.3% to 92.3%. More importantly, the desirable reusability of [email protected] was also evaluated, and the recoveries still maintained above 88.0% even after five re-use cycles. Furthermore, combined with high-performance liquid chromatography (HPLC) analysis, a novel MSPE-HPLC method was developed, enabling the highly selective and sensitive detection of BPA in a wide linear range of 0.5-5000 μg L with a low limit of detection (LOD) of 0.1 μg L. This work contributes a promising method for constructing various functional nanoparticles @[email protected] hybrid materials for applications in many different fields.
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http://dx.doi.org/10.3390/foods11101408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141622PMC
May 2022

Novel ratiometric electrochemical sensing platform with dual-functional poly-dopamine and [email protected] signal amplification for sunset yellow detection in foods.

Food Chem 2022 Oct 10;390:133193. Epub 2022 May 10.

Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, People's Republic of China. Electronic address:

In this work, a novel ratiometric electrochemical sensor based on dual-functional poly-dopamine (PDA) and nickel [email protected] carbon spheres ([email protected]) was successfully developed for sunset yellow (SY) detection. The [email protected] nanocomposite possessing a large specific surface area, good catalytic activity and excellent electrical conductivity was employed for signal amplification. PDA films prepared by electropolymerization acted as an internal reference probe and enhanced the sensitivity of the proposed sensor through electrostatic attraction between SY and PDA. Under optimized conditions, the developed PDA/[email protected]/GCE sensor allowed SY quantification over wide linear range (0.01-100 μM), with a low limit of detection of 0.003 μM. SY recovery tests were carried out in rice vinegar and cooking wine with satisfactory recoveries (83.50-112.80 %). Meanwhile, the content of SY in two kinds of carbonated drinks was determined using the constructed sensor and a UV-Vis spectrometry method, with no significant difference found in the results.
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http://dx.doi.org/10.1016/j.foodchem.2022.133193DOI Listing
October 2022

Neuron-specific enolase promotes stem cell-like characteristics of small-cell lung cancer by downregulating NBL1 and activating the BMP2/Smad/ID1 pathway.

Oncogenesis 2022 Apr 29;11(1):21. Epub 2022 Apr 29.

Department of Medical Oncology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, 510180, Guangzhou, Guangdong, China.

Little is known about the biological functions of neuron-specific enolase (NSE) as a specific biomarker for small-cell lung cancer (SCLC). Herein, we elucidate the effect and mechanism of NSE on SCLC stem cell-like characteristics. Upregulated NSE expression was observed in spheroid cells. The gain-of-function and loss-of-function approaches demonstrated that modulation of NSE positively regulated cell proliferation, drug resistance, spherical clone formation, tumor growth, and stem cell-like characteristics of SCLC cells. Mechanistic studies revealed that NSE might downregulate the expression of neuroblastoma suppressor of tumorigenicity 1 (NBL1) by interacting with NBL1, thereby attenuating the competitive inhibitory effect of NBL1 on BMP2 and enhancing the interaction between BMP2 and BMPR1A; this, in turn, may activate the BMP2/Smad/ID1 pathway and promote SCLC stem cell-like characteristics. Moreover, overexpression of NBL1or knockdown of BMP2 rescued the NSE-induced stem cell-like characteristics. In clinical specimens, NSE expression was positively associated with ALDH1A1 expression and negatively correlated with NBL1 expression. High NSE and ALDH1A1 expressions and low NBL1 expression were correlated with poor prognosis in patients with SCLC. In summary, our study demonstrated that NSE promoted stem cell-like characteristics of SCLC via NBL1 and the activation of the BMP2/Smad/ID1 pathway.
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http://dx.doi.org/10.1038/s41389-022-00396-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054797PMC
April 2022

Photothermal card reader assay using the commercial colloidal gold test strip for the rapid quantitative detection of food hazards.

Mikrochim Acta 2022 02 21;189(3):112. Epub 2022 Feb 21.

Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong, Normal University, College of Life Sciences, Shandong Normal University, Jinan, 250014, People's Republic of China.

The simple and rapid commercial colloidal gold test strip can only be used for qualitative or semi-quantitative detection, accompanied by weak detectability and false negative experimental results. Herein, a photothermal test strip assay which combined test strip with a portable photothermal card reader was established to achieve quantitative detection with excellent detectability. According to the photothermal effect produced by gold nanoparticles (GNPs) captured on the test line, the signal could be recorded by the reader. Thirteen food hazards including veterinary drug residues and pesticide residues were tested; the photothermal detectability in actual samples were about 23 (methyl parathion), 7 (enrofloxacin), 6 (sarafloxacin), 8 (sulfadiazine), 12 (sulfamethazine), 7 (paraquat), 6 (malachite green), 11 (amantadine), 13 (nitrofurazone), 6 (diethylstilbestrol), 12 (estriol), 21 (estrone), and 26 (17β-estradiol) times better than the visual detectability. Our results demonstrated that the photothermal test strip assay could be used for sensitive, rapid, and quantitative detection of residues of food hazards.
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http://dx.doi.org/10.1007/s00604-022-05193-wDOI Listing
February 2022

Competitive Endogenous RNA Network Activates Host Immune Response in SARS-CoV-2-, panH1N1 (A/California/07/2009)-, and H7N9 (A/Shanghai/1/2013)-Infected Cells.

Cells 2022 01 30;11(3). Epub 2022 Jan 30.

Shenzhen Key Laboratory of Pathogen and Immunity, National Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People's Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen 518112, China.

The global outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still ongoing, as is research on the molecular mechanisms underlying cellular infection by coronaviruses, with the hope of developing therapeutic agents against this pandemic. Other important respiratory viruses such as 2009 pandemic H1N1 and H7N9 avian influenza virus (AIV), influenza A viruses, are also responsible for a possible outbreak due to their respiratory susceptibility. However, the interaction of these viruses with host cells and the regulation of post-transcriptional genes remains unclear. In this study, we detected and analyzed the comparative transcriptome profiling of SARS-CoV-2, panH1N1 (A/California/07/2009), and H7N9 (A/Shanghai/1/2013) infected cells. The results showed that the commonly upregulated genes among the three groups were mainly involved in autophagy, pertussis, and tuberculosis, which indicated that autophagy plays an important role in viral pathogenicity. There are three groups of commonly downregulated genes involved in metabolic pathways. Notably, unlike panH1N1 and H7N9, SARS-CoV-2 infection can inhibit the m-TOR pathway and activate the p53 signaling pathway, which may be responsible for unique autophagy induction and cell apoptosis. Particularly, upregulated expression of IRF1 was found in SARS-CoV-2, panH1N1, and H7N9 infection. Further analysis showed SARS-CoV-2, panH1N1, and H7N9 infection-induced upregulation of lncRNA-34087.27 could serve as a competitive endogenous RNA to stabilize IRF1 mRNA by competitively binding with miR-302b-3p. This study provides new insights into the molecular mechanisms of influenza A virus and SARS-CoV-2 infection.
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http://dx.doi.org/10.3390/cells11030487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834034PMC
January 2022

Simultaneous detection of Zika, chikungunya, dengue, yellow fever, West Nile, and Japanese encephalitis viruses by a two-tube multiplex real-time RT-PCR assay.

J Med Virol 2022 06 22;94(6):2528-2536. Epub 2022 Feb 22.

Shenzhen Key Laboratory of Pathogen and Immunity, National Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People's Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen, China.

Due to the concurrent prevalence and increasing risk of coinfection of the clinically important Arboviruses, timely and accurate differential diagnosis is important for clinical management and the epidemiological investigation. A two-tube multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for the simultaneous detection of Zika virus (ZIKV), chikungunya virus (CHIKV), dengue virus (DENV), yellow fever virus (YFV), West Nile virus (WNV), and Japanese encephalitis virus (JEV) was developed and optimized with high specificity and sensitivity. The detection limit for all the six viruses could reach as low as five genome equivalent copies and 2.8 × 10 tissue culture infectious doses (TCID ) for ZIKV, YFV, CHIKV and 2.8 × 10 TCID  for JEV per reaction, with high accuracy and precision (R  > 0.99). The coefficient of variation of intra-assay and inter-assay for our quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay was low, and the obtained positive rates ad C values of this assay were comparable with singleplex commercial kits. Moreover, the multiplex qRT-PCR assay was able to detect possible co-infections without competitive inhibition of target viral genomes. In conclusion, our rapid, sensitive, cost-effective multiplex qRT-PCR will be of great use for differential diagnosis in a clinical setting and epidemiological investigation during surveillance.
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http://dx.doi.org/10.1002/jmv.27658DOI Listing
June 2022

Longitudinal analysis of antibody dynamics in COVID-19 convalescents reveals neutralizing responses up to 16 months after infection.

Nat Microbiol 2022 03 7;7(3):423-433. Epub 2022 Feb 7.

Shenzhen Key Laboratory of Pathogen and Immunity, National Clinical Research Center for infectious disease, State Key Discipline of Infectious Disease, Shenzhen Third People's Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen, China.

Elucidating the dynamics of the neutralizing antibody (nAb) response in coronavirus disease 2019 (COVID-19) convalescents is crucial in controlling the pandemic and informing vaccination strategies. Here we measured nAb titres across 411 sequential plasma samples collected during 1-480 d after illness onset or laboratory confirmation (d.a.o.) from 214 COVID-19 convalescents, covering the clinical spectrum of disease and without additional exposure history after recovery or vaccination against SARS-CoV-2, using authentic SARS-CoV-2 microneutralization (MN) assays. Forty-eight samples were also tested for neutralizing activities against the circulating variants using pseudotyped neutralization assay. Results showed that anti-RBD IgG and MN titres peaked at ~120 d.a.o. and subsequently declined, with significantly reduced nAb responses found in 91.67% of COVID-19 convalescents (≥50% decrease in current MN titres compared with the paired peak MN titres). Despite this decline, majority of the COVID-19 convalescents maintained detectable anti-RBD IgG and MN titres at 400-480 d.a.o., with undetectable neutralizing activity found in 14.41% (16/111) of the mild and 50% (5/10) of the asymptomatic infections at 330-480 d.a.o. Persistent antibody-dependent immunity could provide protection against circulating variants after one year, despite significantly decreased neutralizing activities against Beta, Delta and Mu variants. In conclusion, these data show that despite a marked decline in neutralizing activity over time, nAb responses persist for up to 480 d in most convalescents of symptomatic COVID-19, whereas a high rate of undetectable nAb responses was found in convalescents from asymptomatic infections.
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http://dx.doi.org/10.1038/s41564-021-01051-2DOI Listing
March 2022

Low concentrations of 17β-estradiol exacerbate tamoxifen resistance in breast cancer treatment through membrane estrogen receptor-mediated signaling pathways.

Environ Toxicol 2022 Mar 25;37(3):514-526. Epub 2021 Nov 25.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.

The present study aims to discover the influences of tamoxifen and 17β-estradiol (E2) on tamoxifen-resistant (TamR) patients in vitro. Herein, we established a stabilized TamR MCF-7 cell line at 1 μM via gradient concentrations of tamoxifen cultivation. The expression changes of four ER subtypes (ERα66, ERβ, ERα36 and GPR30) were found to bring about tamoxifen resistance. Moreover, the generation of tamoxifen resistance involved in apoptosis escape via a reactive oxygen species-regulated p53 signaling pathway. Interestingly, E2 at environmental concentrations (0.1-10 nM) could activate the expression of both ERα36 and GPR30, and then stimulate the phosphorylation of ERK1/2 and Akt, resulting in cell growth promotion. Cell migration and invasion promotion, apoptosis inhibition, and cell cycle G1-S progression are involved in such proliferative effects. Conversely, the application of specific antagonists of ERα36 and GPR30 could restore tamoxifen's sensitivity as well as partially offset E2-mediated proliferation. In short, overexpression of ERα36 and GPR30 not only ablate tamoxifen responsiveness but also could promote tumor progression of TamR breast cancer under estrogen conditions. These results provided novel insights into underlying mechanisms of tamoxifen resistance and the negative effects of steroid estrogens at environmental concentrations on TamR MCF-7 cells, thus generating new thoughts for future management of ER-positive breast cancer.
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http://dx.doi.org/10.1002/tox.23417DOI Listing
March 2022

Sensitive analytical detection of nitrite using an electrochemical sensor with STAB-functionalized [email protected] for signal amplification.

Food Chem 2022 Mar 7;372:131356. Epub 2021 Oct 7.

Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, People's Republic of China. Electronic address:

An electrochemical sensor based on stearyl trimethyl ammonium bromide - functionalized niobium [email protected] carbon nanotubes ([email protected]) for signal amplification was successfully constructed for sensitive detection of nitrite (NO). Niobium [email protected] carbon nanotubes ([email protected]) with high electrical conductivity and water dispersibility were first prepared in a one-pot hydrothermal synthesis, after which cationic STAB was added to overcome the negative surface charge on the [email protected] The electrostatic attraction between [email protected] and NO was improved by the STAB, which enhanced the sensitivity of the constructed sensor for NO. Under optimized conditions, [email protected]/GCE exhibited excellent analytical performance for detection NO with two wide liner ranges (0.1-100 μmol L and 100-2000 μmol L) and a limit of detection of 0.022 μmol L. Nitrite recovery tests in milk and spinach samples showed recoveries in the range of 89.82-104.52%. The NO residues in ham and pickled vegetable (cedrela sinensis) samples were analysed using the presented sensor and a spectrophotometric method, with no significant difference found between the results of the two methods.
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http://dx.doi.org/10.1016/j.foodchem.2021.131356DOI Listing
March 2022

Effects of whole grain intake on glycemic traits: A systematic review and meta-analysis of randomized controlled trials.

Crit Rev Food Sci Nutr 2021 Nov 18:1-20. Epub 2021 Nov 18.

Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Shandong Engineering Technology Research Center of food for Special Medical Purpose/Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture and Rural Affairs, Jinan, China.

Whole grains (WGs) may have various health benefits, including lowering blood glucose and improving insulin sensitivity. To conduct a meta-analysis of the effects of WGs compared with non-WGs on changes in fasting glucose, fasting insulin, glycated hemoglobin (HbA1c), and homeostasis model assessment of insulin resistance (HOMA-IR). A systematic literature search was performed for all published randomized controlled trials on the effects of WG intake on fasting glucose, fasting insulin, HbA1c and HOMA-IR response up to February 2021. Weighted mean differences (WMD) were calculated. Pre-specified subgroup and univariate meta-regression analyses were explored to identify the sources of heterogeneity. Sensitivity analysis and bias analysis were conducted to appraise study quality. Among 12,435 articles screened for eligibility, data were extracted from 48 articles. Meta-analysis of 4,118 participants showed that WG consumption resulted in a significant reduction in fasting glucose by -0.15 mmol/L, fasting insulin by -2.71 pmol/L, HbA1c by -0.44%, and HOMA-IR by -0.28, respectively. Compared with mixed grains, brown rice, and wheat, oats were significantly lower on marker of glycemic. Besides, multiple interventions per day consolidated effectiveness of WGs. WG consumption decreased the levels of fasting glucose, fasting insulin, HbA1c, and HOMA-IR compared with non-WG consumption.
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http://dx.doi.org/10.1080/10408398.2021.2001429DOI Listing
November 2021

Synthesis of magnetic hydrochar from Fenton sludge and sewage sludge for enhanced anaerobic decolorization of azo dye AO7.

J Hazard Mater 2022 02 28;424(Pt C):127622. Epub 2021 Oct 28.

Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, Chemical Pollution Control Engineering Research Center of Ministry of Education, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

A novel magnetic hydrochar synthesized from Fenton sludge (FS) and sewage sludge (SS) was employed in the anaerobic decolorization of acid orange 7 (AO7). The stable presence of FeO in magnetic hydrochar was confirmed by physicochemical characterization. The degradation efficiency of AO7 in the anaerobic system with the addition of hydrochar prepared in an optimal proportion (SS:FS=1:3, named as HC-1:3) could reach 98.55%, which was 1.91 times higher than the control system. Particularly, superior electrical conductivity, electron transport system activity and azo reductase activity of the sludge in anaerobic system with HC-1:3 were achieved. The redox of Fe(Ⅲ)/Fe(Ⅱ) in anaerobic system was realized by dissimilatory iron-reducing bacteria enriched with HC-1:3. According to the six-cycle batch experiments and 120-day continuous-flow UASB experiments, the addition of HC-1:3 into the anaerobic system facilitated the diversity of microbiological community and increased the ecological stability of anaerobic system. The possible electron transfer mechanism involving in the magnetic hydrochar-based anaerobic system for AO7 removal was speculated preliminarily. The as-prepared magnetic hydrochar not only showed a promising future in anaerobic system for recalcitrant contaminants degradation, but also provided a new approach for the resource utilization of FS and SS.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127622DOI Listing
February 2022

Practical Experiences of Delivering Multidrug-Resistant Tuberculosis Comprehensive Supportive Care Services in China.

China CDC Wkly 2021 Jun;3(26):566-568

National Center for Tuberculosis Control and Prevention, China CDC, Beijing, China.

The World Health Organization consolidated guidelines on recommend care for tuberculosis (TB) and support for multidrug-resistant TB (MDR-TB) patients. But guidelines have not provided detailed guidance or tools for health services providers to implement comprehensive patient care.



China CDC and the United States Agency for International Development-funded Control and Prevention of MDR-TB program introduced a differentiated and personalized comprehensive and supportive care services (CSC) to improve treatment adherence.



The CSC model helps MDR-TB patients complete treatment and improve treatment success rates, and scaling up the program and implementation in other parts of the country is worth consideration.
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http://dx.doi.org/10.46234/ccdcw2021.146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392942PMC
June 2021

Double-dose responses of Scenedesmus capricornus microalgae exposed to humic acid.

Sci Total Environ 2022 Feb 25;806(Pt 1):150547. Epub 2021 Sep 25.

Faculty of Environmental Science & Engineering, Kunming University of Science and Technology, Kunming 650500, PR China. Electronic address:

Dissolved organic matter (DOM) has been found to attenuate the ecotoxicity of various environmental pollutants, but research on its own toxic effects in aquatic ecosystems has been very limited. Herein, the toxic effects of humic acid (HA), a represent DOM typically found in natural waters, on the freshwater alga Scenedesmus capricornus were investigated. As result, HA exerted a double-dose effect on the growth of Scenedesmus capricornus. At HA concentrations below 2.0 mgC/L, the growth of Scenedesmus capricornus was slightly promoted, as was the synthesis of chlorophyll and macromolecules in the algae. Moreover, S. capricornus can maintain its growth by secreting fulvic acid as a nutrient carbon source. However, the growth of Scenedesmus capricornus was significantly inhibited when HA was beyond 2.0 mgC/L. The main mechanisms of humic acid's toxicity were membrane damage and oxidative stress. Particularly, when the oxidative stress exceeds the algae's carrying capacity, the synthesis of EPS is greatly inhibited and HA damage results. Taken together, DOM may have both positive and negative effects on aquatic ecosystems.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150547DOI Listing
February 2022

A surface-imprinted surface-enhanced Raman scattering sensor for histamine detection based on dual semiconductors and Ag nanoparticles.

Food Chem 2022 Feb 27;369:130971. Epub 2021 Aug 27.

Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China. Electronic address:

A highly sensitive molecularly imprinted surface-enhanced Raman scattering (SERS) sensor was developed for selective detection of histamine. A combination of two semiconductors and Ag nanoparticles (NPs) was used as the SERS substrate. The SERS was induced by Ag NPs plasmon resonances as well as charge-transfer between the semiconductors and the Ag NPs. The Raman intensity and the logarithm of the histamine concentration were linear over the range 10-10 mol L. The sensor exhibited good selectivity and had a sensitivity limit of 3.088 × 10 mol L. Histamine was detected in a spiked liquor sample, and its recoveries were in the range of 89.89%-109.18%.
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http://dx.doi.org/10.1016/j.foodchem.2021.130971DOI Listing
February 2022

Prognostic Roles of Phosphofructokinase Platelet in Clear Cell Renal Cell Carcinoma and Correlation with Immune Infiltration.

Int J Gen Med 2021 20;14:3645-3658. Epub 2021 Jul 20.

Department of Urology, the First Hospital of Jilin University, Changchun, 130021, Jilin, People's Republic of China.

Background: Abnormal expression of phosphofructokinase platelet (PFKP) has been reported in various cancer types. However, the role of PFKP in clear cell renal cell carcinoma (ccRCC) remains unclear.

Methods: In this study, the PFKP expression levels in various cancers were systemically described by integrating multiple kinds of publicly available databases. The relationship between PFKP expression and clinical prognosis of ccRCC patients was analyzed based on the TCGA database. Furthermore, PFKP-related genes and the top 10 hub genes were identified. The enrichment analysis, PPI network, and the relationship between PFKP and tumor-infiltrating immune cells were conducted to explore why PFKP was associated with clinical outcomes in ccRCC patients.

Results: PFKP was significantly highly expressed in kidney cancer, especially in ccRCC. Moreover, patients with low expression of PFKP were correlated with poor 5-year and 10-year overall survival (OS) ( < 0.05). Low PFKP expression was a risk factor associated with decreased OS in subgroups including males, females, grade 3-4, and stage III-IV (all < 0.05). GO and KEGG enrichment analyses showed that 10 hub genes were mainly enriched in the tumor immune response. Finally, PFKP expression level was highly correlated with the infiltration of B cell, CD8 T cell, CD4 T cell, macrophage, neutrophil, and dendritic cell.

Conclusion: In short, our findings suggested that PFKP is highly expressed in ccRCC significantly and facilitated tumor immune response which in turn associated with a good prognosis.
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http://dx.doi.org/10.2147/IJGM.S321337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312753PMC
July 2021

Cytokine/Chemokine Expression Is Closely Associated Disease Severity of Human Adenovirus Infections in Immunocompetent Adults and Predicts Disease Progression.

Front Immunol 2021 7;12:691879. Epub 2021 Jun 7.

Shenzhen Key Laboratory of Pathogen and Immunity, National Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People's Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen, China.

Increasing human Adenovirus (HAdV) infections complicated with acute respiratory distress syndrome (ARDS) even fatal outcome were reported in immunocompetent adolescent and adult patients. Here, we characterized the cytokine/chemokine expression profiles of immunocompetent patients complicated with ARDS during HAdV infection and identified biomarkers for disease severity/progression. Forty-eight cytokines/chemokines in the plasma samples from 19 HAdV-infected immunocompetent adolescent and adult patients (ten complicated with ARDS) were measured and analyzed in combination with clinical indices. Immunocompetent patients with ARDS caused by severe acute respiratory disease coronavirus (SARS-CoV)-2, 2009 pandemic H1N1 (panH1N1) or bacteria were included for comparative analyses. Similar indices of disease course/progression were found in immunocompetent patients with ARDS caused by HAdV, SARS-CoV-2 or panH1N infections, whereas the HAdV-infected group showed a higher prevalence of viremia, as well as increased levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatine kinase (CK). Expression levels of 33 cytokines/chemokines were increased significantly in HAdV-infected patients with ARDS compared with that in healthy controls, and many of them were also significantly higher than those in SARS-CoV-2-infected and panH1N1-infected patients. Expression of interferon (IFN)-γ, interleukin (IL)-1β, hepatocyte growth factor (HGF), monokine induced by IFN-γ (MIG), IL-6, macrophage-colony stimulating factor (M-CSF), IL-10, IL-1α and IL-2Ra was significantly higher in HAdV-infected patients with ARDS than that in those without ARDS, and negatively associated with the ratio of the partial pressure of oxygen in arterial blood/fraction of inspired oxygen (PaO/FiO). Analyses of the receiver operating characteristic curve (ROC) showed that expression of IL-10, M-CSF, MIG, HGF, IL-1β, IFN-γ and IL-2Ra could predict the progression of HAdV infection, with the highest area under the curve (AUC) of 0.944 obtained for IL-10. Of note, the AUC value for the combination of IL-10, IFN-γ, and M-CSF reached 1. In conclusion, the "cytokine storm" occurred during HAdV infection in immunocompetent patients, and expression of IL-10, M-CSF, MIG, HGF, IL-1β, IFN-γ and IL-2Ra was closely associated with disease severity and could predict disease progression.
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http://dx.doi.org/10.3389/fimmu.2021.691879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215364PMC
July 2021

A novel SERS sensor for the ultrasensitive detection of kanamycin based on a Zn-doped carbon quantum dot catalytic switch controlled by nucleic acid aptamer and size-controlled gold nanorods.

Food Chem 2021 Nov 1;362:130261. Epub 2021 Jun 1.

Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China. Electronic address:

In this study, a novel surface enhanced Raman spectroscopy (SERS) sensor was developed for the ultrasensitive determination of kanamycin in foods. The sensor used two distinct signal amplification strategies, namely the surface plasmon resonance of gold nanorods and a Zn-doped carbon quantum dots catalytic cascade oxidation-reduction reaction switch controlled by a nucleic acid aptamer. Under optimized experimental conditions, the SERS sensor demonstrated a linear range of 10 to 10 g mL for the detection of kanamycin, with a limit of detection of 3.03 × 10 g mL. Experiments with antibiotics structurally similar to kanamycin and interferrants revealed that the sensor had excellent selectivity. Milkpowder and honey samples spiked with kanamycin were assayed, with recoveries ranging from 84.1% to 107.2% and a relative standard deviation of 0.74% to 2.81% being obtained. Quantification of kanamycin in milk samples revealed no significant difference between the results obtained with the sensor and by HPLC.
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http://dx.doi.org/10.1016/j.foodchem.2021.130261DOI Listing
November 2021

Profiles of immune cell infiltration and immune-related genes in the tumor microenvironment of esophageal squamous cell carcinoma.

BMC Med Genomics 2021 03 10;14(1):75. Epub 2021 Mar 10.

Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun, 130021, Jilin, People's Republic of China.

Background: As a complex system participating in tumor development and progression, the tumor microenvironment was poorly understood in esophageal cancer especially squamous cell carcinoma (ESCC).

Methods: ESTIMATE algorithm is used to investigate tumor-infiltrating immune cells and prognostic genes which were associated with the tumor microenvironment in ESCC.

Results: Based on the immune and stromal scores, ESCC samples were divided into high and low score groups and 299 overlapping differentially expressed genes were identified. Functional enrichment analysis showed that these genes were mainly involved in muscle-related function. Prognostic genes including COL9A3, GFRA2, and VSIG4 were used to establish a risk prediction model using Cox regression analyses. Then multivariate analysis showed that COL9A3 was an independent discriminator of a better prognosis. Kaplan-Meier survival analysis showed that the expression of COL9A3 was significantly correlated with the overall survival of ESCC patients. The area under the curve for the risk model in predicting 1- and 3- year survival rates were 0.660 and 0.942, respectively. The risk score was negatively correlated with plasma cells, while positively correlated with the proportions of activated CD4 memory T cells, M1 Macrophages and M2 Macrophages (p < 0.001 for each comparison). Gene set enrichment analysis suggested that both immune response and immune system process gene sets were significantly enriched in high-risk group.

Conclusions: Our study provided a comprehensive understanding of the TME in ESCC patients. The establishment of the risk model is valuable for the early identification of high-risk patients to facilitate individualized treatment and improve the possibility of immunotherapy response.
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http://dx.doi.org/10.1186/s12920-021-00928-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944628PMC
March 2021

Modified humic acids mediate efficient mineralization in a photo-bio-electro-Fenton process.

Water Res 2021 Feb 9;190:116740. Epub 2020 Dec 9.

Environmental Engineering and Science Program, Department of Chemical and Environmental Engineering (ChEE), 705 Engineering Research Center, University of Cincinnati, Cincinnati, OH, 45221-0012 USA.

Humic acids (HA) are common mediators in redox reactions in the aquatic environment. The structures and properties of HA are greatly influenced by environmental factors such as external electrons. In this study, qualitative changes in electron-modified HA and the underlying mechanisms were reported, which not only contribute to understanding the fate of HA and their impact on organic pollutants, but could facilitate their potential use for water purification. The photochemical activity and electron-donating capacity of HA were improved due to the increase of phenolic and carboxyl components via the reduction modification by electrons, creating a novel and efficient photo-bio-electro-Fenton system mediated by HA under neutral conditions without the use of hydrogen peroxide (HO). The in-situ continuous production of HO ensured an adequate supply of hydroxyl radicals in this coupled system, achieving mineralization (92%) of HA and 17α-ethinylestradiol (EE2), a common synthetic estrogen with high estrogenic potency. Two degradation pathways with five degradation intermediates of EE2 were identified in our study. Effluents from the coupled system showed decreased endocrine-disrupting activity. Our findings demonstrated a new approach for the in-situ modification and potential use of HA for water treatment and particularly the concurrent degradation of HA and organic pollutants through a photo-bioelectrochemical system mediated by HA.
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http://dx.doi.org/10.1016/j.watres.2020.116740DOI Listing
February 2021

A semi-covalent molecularly imprinted fluorescent sensor for highly specific recognition and optosensing of bisphenol A.

Anal Methods 2021 01 15;13(1):133-140. Epub 2020 Dec 15.

Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, P. R. China.

A novel mesoporous fluorescent molecularly imprinted sensor for selective detection of bisphenol A (BPA) in food materials was fabricated via a semi-covalent imprinting method. The imprinting precursor that served as an alternative template molecule for BPA was prepared via thermally reversible isocyanate bonding, which effectively improved the imprinting efficiency for the molecularly imprinted sensor. Carbon dots (CDs) were embedded in mesoporous silica as signal recognition elements that exhibited quenching upon BPA binding. Subsequently, through the sol-gel process, the molecularly imprinted layer was coated on the CDs silica layer and provided specific recognition sites for BPA. The composite of CDs embedded in the mesoporous molecularly imprinted polymer ([email protected]) was characterized with scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller measurements and thermogravimetric analysis. The mechanism of carbon dots quenching and the high selectivity of [email protected] towards BPA were explored. The linear response range of the sensor was from 0.025 mg L to 2 mg L with a limit of detection of 0.016 mg L. The method was successfully applied for the determination of food samples and recoveries ranged from 92.5% to 101.1%. The BPA contents in actual samples were determined using high performance liquid chromatography and the proposed sensor, showing no significant difference between the two methods.
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http://dx.doi.org/10.1039/d0ay01822hDOI Listing
January 2021

Recycle of Fenton sludge through one-step synthesis of aminated magnetic hydrochar for Pb removal from wastewater.

J Hazard Mater 2021 03 20;406:124581. Epub 2020 Nov 20.

Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address:

In order to achieve proper disposal of Fenton sludge, a new recycle method for preparing adsorbents based on one-step hydrothermal carbonization synthesis of aminated hydrochar from Fenton sludge (AHFS) was developed. It was found that AHFS prepared at 340 °C for 60 min showed Pb adsorption capacity as high as 359.83 mg g. Adsorption kinetics and thermodynamics results indicated that chemical interaction, intra-particle diffusion and monolayer homogeneous surface of AHFS dominated in adsorption process. The contribution proportion of different mechanisms, including cation-exchange (43.15%), acidic groups complexation (28.17%) and amino groups complexation (24.06%) to overall Pb adsorption, demonstrated that complexation of surface functional groups played the dominated role in the adsorption process. Especially, the addition of amino was conducive to the increased adsorption capacity of hydrochar. In addition, according to the regeneration test, the magnetic AHFS exhibited a satisfactory reproducibility and recyclability. These findings illustrated that the synthesis of aminated magnetic hydrochar not only provided an innovative and efficient heavy metal adsorbent to remove Pb from wastewater, but also explored a new method for the resource utilization of Fenton sludge.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124581DOI Listing
March 2021

Explore prognostic biomarker of bladder cancer based on competing endogenous network.

Biosci Rep 2020 12;40(12)

Department of Urology, the First Hospital of Jilin University, Changchun 130021, Jilin, China.

Bladder cancer (BC) is the most common tumor of the urinary tract. Increasing evidence showed that long non-coding RNA (lncRNA) is a critical regulator in cancer development and progression. However, the functions of lncRNAs in the development of BC remain mostly undefined. In the present study, based on RNA sequence profiles from The Cancer Genome Atlas database, we identified 723 lncRNAs, 157 miRNAs, and 1816 mRNAs aberrantly expressed in BC tissues. A competing endogenous RNA network, including 49 lncRNAs, 17 miRNAs, and 36 mRNAs, was then established. The functional enrichment analyses showed that the mRNAs in the ceRNA network mainly participated in 'regulation of transcription' and 'pathways in cancer'. Moreover, the Cox regression analyses demonstrated that three lncRNAs (AC112721.1, TMPRSS11GP, and ADAMTS9-AS1) could serve as independent risk factors. We established a risk prediction model with these lncRNAs. Kaplan-Meier curve analysis showed that high-risk patients' prognosis was lower than that of low-risk patients (P=0.001). The present study provides novel insights into the lncRNA-mediated ceRNA network and the potential of lncRNAs to be candidate prognostic biomarkers in BC, which could help better understand the pathological changes and pathogenesis of BC and be useful for clinical studies in the future.
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http://dx.doi.org/10.1042/BSR20202463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711062PMC
December 2020

Abundance and distribution characteristics of microplastic in plateau cultivated land of Yunnan Province, China.

Environ Sci Pollut Res Int 2021 Jan 26;28(2):1675-1688. Epub 2020 Aug 26.

Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China.

Microplastic pollution in cultivated soil has received increasing attention recently. There may be more serious microplastic abundance but little research has been done in cultivated soil in plateau areas. To survey the pollution characteristics of microplastics in inland cultivated soil, 100 soil samples collected from 10 counties of Yunnan Province were investigated through density separation and microscopic examination. The research results showed that microplastic abundance was in the range of 0.9 × 10 to 40.8 × 10 particles (kg Ds) with average abundance of 9.8 × 10 particles (kg Ds). Moreover, compared with other studies on sediments, it was found that microplastic abundance in inland soil was one order of magnitude higher than that in offshore sediments. The use of plastic mulch and its long-term residue in cultivated soil was an important reason for microplastic pollution. In this survey, various morphologies of microplastics existed, including fragment (78.3%), transparent/translucency (49.7%), and micro-size microplastics (< 500 μm) (89.3%). And the microplastic morphologies occurred in different degrees of aging phenomenon under the influence of the environment factors such as ultraviolet radiation. The findings provided the pollution status of microplastics in cultivated soil, and more attention should be paid to inland soil microplastic pollution. Grapical abstract.
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http://dx.doi.org/10.1007/s11356-020-10527-3DOI Listing
January 2021

Prognostic Value of Immune-Related Genes in the Tumor Microenvironment of Bladder Cancer.

Front Oncol 2020 28;10:1302. Epub 2020 Jul 28.

Department of Urology, The First Hospital of Jilin University, Changchun, China.

The tumor microenvironment (TME) is a complex system that plays an important role in tumor development and progression, but the current knowledge about its effect on bladder cancer (BC) is scarce. In this study, we performed a comprehensive analysis of the relationship between the TME and gene expression profiles to identify prognostic biomarkers for BC. The ESTIMATE algorithm was used to calculate immune and stromal scores of BC patients who were obtained from the Gene Expression Omnibus database. We found that the immune and stromal scores were associated with clinical characteristics and the prognosis of BC patients. Based on these scores, 104 immune-related differentially expressed genes were identified. Further, functional enrichment analysis revealed that these genes were mainly involved in the immune-related biological processes and signaling pathways. Three prognostic genes were then identified and used to establish a risk prediction model using Cox regression analyses. Kaplan-Meier survival analysis showed that the expression levels of COL1A1, COMP, and SERPINE2 significantly correlated with cancer-specific survival and overall survival of BC patients. Additionally, we validated the prognostic values of these genes using two independent cohorts from The Cancer Genome Atlas and Gene Expression Omnibus databases. Finally, the relationships between the three prognostic genes and several immune cells were evaluated using Tumor Immune Estimation Resource, indicating that the expression levels of COL1A1, COMP, and SERPINE2 correlated positively with the tumor infiltration levels of CD4 T cells and macrophages. In conclusion, the current study comprehensively analyzed the TME and presented immune-related prognostic genes for BC, providing new insights into immunotherapeutic strategies for BC patients.
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http://dx.doi.org/10.3389/fonc.2020.01302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399341PMC
July 2020

Ultrasensitive determination of sulfathiazole using a molecularly imprinted electrochemical sensor with CuS microflowers as an electron transfer probe and [email protected] for signal amplification.

Food Chem 2020 Dec 25;332:127376. Epub 2020 Jun 25.

Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, People's Republic of China. Electronic address:

In this work, a molecularly imprinted sensor employing copper sulfide (CuS) as a novel signal probe was successfully developed for ultrasensitive and selective determination of sulfathiazole (STZ). The reduction signals of Cu produced in the process of electron transfer of CuS containing large amounts of Cu are easy to be captured, which provide high electrochemical signals. Moreover, gold [email protected] organic framework with excellent conductivity was introduced on the electrode surface for signal amplification and facilitating electron transfer processes of CuS. Under optimized testing conditions, the proposed sensor offered a linear DPV response to STZ over a very wide concentration range (1.0 × 10 to 1.0 × 10 mol L), with a limit of detection of 4.3 × 10 mol L. Fodder and mutton samples spiked with STZ were analyzed using this sensor, and the satisfactory recoveries ranging from 83.0% to 107.2% were obtained. In addition, the proposed sensor was used to determine the concentration of STZ in chicken liver and pork liver, with quantification results being near identical to those determined by high-performance liquid chromatography.
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http://dx.doi.org/10.1016/j.foodchem.2020.127376DOI Listing
December 2020
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