Publications by authors named "Zhiwei Zhang"

501 Publications

Evaluating the Impact of Incentives on Clinical Trial Participation: Protocol for a Mixed Methods, Community-Engaged Study.

JMIR Res Protoc 2021 Nov 23;10(11):e33608. Epub 2021 Nov 23.

Department of Social Medicine, Population and Public Health, University of California, Riverside, Riverside, CA, United States.

Background: Monetary incentives in research are frequently used to support participant recruitment and retention. However, there are scant empirical data regarding how researchers decide upon the type and amount of incentives offered. Likewise, there is little guidance to assist study investigators and institutional review boards (IRBs) in their decision-making on incentives. Monetary incentives, in addition to other factors such as the risk of harm or other intangible benefits, guide individuals' decisions to enroll in research studies. These factors emphasize the need for evidence-informed guidance for study investigators and IRBs when determining the type and amount of incentives to provide to research participants.

Objective: The specific aims of our research project are to (1) characterize key stakeholders' views on and assessments of incentives in biomedical HIV research; (2) reach consensus among stakeholders on the factors that are considered when choosing research incentives, including consensus on the relative importance of such factors; and (3) pilot-test the use of the guidance developed via aims 1 and 2 by presenting stakeholders with vignettes of hypothetical research studies for which they will choose corresponding incentive types.

Methods: Our 2-year study will involve monthly, active engagement with a stakeholder advisory board of people living with HIV, researchers, and IRB members. For aim 1, we will conduct a nationwide survey (N=300) among people living with HIV to understand their views regarding the incentives used in HIV research. For aim 2, we will collect qualitative data by conducting focus groups with people living with HIV (n=60) and key informant interviews with stakeholders involved in HIV research (people living with HIV, IRB members, and biomedical HIV researchers: n=36) to extend and deepen our understanding of how incentives in HIV research are perceived. These participants will also complete a conjoint analysis experiment to gain an understanding of the relative importance of key HIV research study attributes and the impact that these attributes have on study participation. The data from the nationwide survey (aim 1) will be triangulated with the qualitative and conjoint analysis data (aim 2) to create 25 vignettes that describe hypothetical HIV research studies. Finally, individuals from each stakeholder group will select the most appropriate incentive that they feel should be used in each of the 25 vignettes (aim 3).

Results: The stakeholder advisory board began monthly meetings in March 2021. All study aims are expected to be completed by December 2022.

Conclusions: By studying the role of incentives in HIV clinical trial participation, we will establish a decision-making paradigm to guide the choice of incentives for HIV research and, eventually, other types of similar research and facilitate the ethical recruitment of clinical research participants.

Trial Registration: ClinicalTrials.gov NCT04809636; https://clinicaltrials.gov/ct2/show/NCT04809636.

International Registered Report Identifier (irrid): DERR1-10.2196/33608.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2196/33608DOI Listing
November 2021

C/EBPZ modulates the differentiation and proliferation of preadipocytes.

Int J Obes (Lond) 2021 Nov 17. Epub 2021 Nov 17.

NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases of First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China.

Background/objectives: This study investigated the functions of CCAAT/enhancer-binding protein zeta (C/EBPZ; Gene ID: 10153) in adipose tissue.

Subjects/methods: Bioinformatics analysis were used to study the expression pattern of C/EBPZ in human adipose tissue. The expression and function of C/EBPZ in adipose tissue were further studied using chicken as animal model in vivo and in vitro.

Results: The human C/EBPZ transcripts were greater and more stable in subcutaneous adipose tissue than in visceral adipose tissue (P < 0.01), and they were increased with age in adipose tissue (P < 0.05). In addition, the chicken C/EBPZ transcripts (C/EBPZ /ACTB) of visceral (abdominal) adipose tissue were significantly different between fat and lean broilers and decreased with age during development (P < 0.01). RNA-seq analysis showed that the C/EBPZ overexpression associated with adipose tissue development and DNA replication in chicken preadipocytes (P < 0.05). Additionally, overexpression of chicken C/EBPZ inhibited preadipocytes differentiation and promoted preadipoytes proliferation in vitro (P < 0.05). In addition, C/EBPZ overexpression suppressed the promoter activities of PPARγ, C/EBPα, FASN and LPL, and promoted the promoter activities of GATA2 and FABP4 in chicken preadipocytes (P < 0.05).

Conclusions: C/EBPZ modulated the differentiation and proliferation of preadipocytes, and it might be a new negative regulator of adipogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41366-021-01020-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597877PMC
November 2021

The ultrafast, high-pitch turbo FLASH mode of third-generation dual-source CT: Effect of different pitch and corresponding SFOV on image quality in a phantom study.

J Appl Clin Med Phys 2021 Nov 9. Epub 2021 Nov 9.

Department of Radiology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Purpose: To investigate the effect of different pitches and corresponding scan fields of view (SFOVs) on the image quality in the ultrafast, high-pitch turbo FLASH mode of the third-generation dual-source CT using an anthropomorphic phantom.

Methods: The phantom was scanned using the ultrafast, high-pitch turbo FLASH protocols of the third-generation dual-source CT with the different pitches and corresponding SFOVs (pitches: 1.55 to 3.2 with increments of 0.1, SFOVs: 50 cm to 35.4 cm). The objective parameters such as the CT number, image noises, signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and artifacts index (AI), and image features from the head, chest, and abdomen were compared between the CT images with a pitch of 1.55 and SFOV of Ø 50 cm and a pitch of 3.2 and SFOV of Ø 35.4 cm. Then, the 18 series of CT images of the head, chest, and abdomen were evaluated by three radiologists independently.

Results: The differences in the CT numbers were not statically significant between the CT images with a pitch of 1.55 and SFOV of Ø 50 cm and a pitch of 3.2 and SFOV of Ø 35.4 cm from most body parts and potential combinations (p > 0.05), Most of the image noises and the AI from the images with the pitch of 1.55 were significantly lower than those with the pitch of 3.2 (p < 0.05), and the SNR and CNR from the images with the pitch of 1.55 were higher than those with the pitch of 3.2. There were significant differences in the first-order features and texture features of the head (59.3%, 28.3%), chest (66%, 35.7%), and abdomen (71.6%, 64.7%) (p < 0.05). The subjective image quality was excellent when the pitch was less than 2.0 and gradually decreased with the increasing pitch. In addition, the image quality decreased significantly when the pitch was higher than 3.0 (all k≥0.69), especially in the head and chest.

Conclusions: In the ultrafast, high-pitch turbo FLASH mode of the third-generation DSCT, increasing the pitch and lowering the corresponding SFOV will change the image features and cause more artifacts degrading the image quality. Specific to the clinical needs, decreasing the pitch not only can expand the SFOV but also can improve the image quality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/acm2.13466DOI Listing
November 2021

Surface modification to enhance cell migration on biomaterials and its combination with 3D structural design of occluders to improve interventional treatment of heart diseases.

Biomaterials 2021 Oct 27;279:121208. Epub 2021 Oct 27.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200438, China. Electronic address:

The dominant source of thromboembolism in heart comes from the left atrial appendage (LAA). An occluder can close LAA and significantly reduce the risk of strokes, particularly for those patients with atrial fibrillation. However, it is technically challenging to fabricate an LAA occluder that is appropriate for percutaneous implantation and can be rapidly endothelialized to accomplish complete closure and avoid severe complication. Hypothesizing that a fast migration rate of endothelial cells on the implant surface would lead to rapid endothelialization, we fabricated an LAA occlusion device for interventional treatment with a well-designed 3D architecture and a nanoscale 2D coating. Through screening of biomaterials surfaces with cellular studies in vitro including cell observations, qPCR, RNA sequencing, and implantation studies in vivo, we revealed that a titanium-nitrogen nanocoating on a NiTi alloy promoted high migration rate of endothelial cells on the surface. The effectiveness of this first nanocoating LAA occluder was validated in animal experiments and a patient case, both of which exhibited successful implantation, fast sealing and long-term safety of the device. The mechanistic insights gained in this study will be useful for the design of medical devices with appropriate surface modification, not necessarily for improved cell adhesion but sometimes for enhanced cell migration.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biomaterials.2021.121208DOI Listing
October 2021

The complete chloroplast genome of maxim. (Asteraceae), an alpine Tibetan herb.

Mitochondrial DNA B Resour 2021 7;6(11):3144-3145. Epub 2021 Oct 7.

TAAHC-SWU Medicinal Plant Joint R&D Centre, Tibetan Collaborative Innovation Centre of Agricultural and Animal Husbandry Resources, Food Science College, Tibet Agriculture & Animal Husbandry University, Nyingchi, China.

is an important traditional Tibetan medicinal plant in China. In this study, we assembled the complete chloroplast (cp) genome of . The complete chloroplast genome is a circular molecular structure of 152,257 bp in length with coding GC 37.93%, consisting of two inverted repeats (25,204 bp) separated by a large single-copy region (83,334 bp) and a small single-copy region (18,515 bp). The complete chloroplast genome of contained 130 genes, including 87 protein-coding genes, 35 tRNA genes, and eight rRNA genes. Phylogenetic analysis shows that is most closely related to and . The complete chloroplast genome sequence of facilitates the phylogenetic studies of Asteraceae.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1984328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567947PMC
October 2021

Surgical methods of treatment for cholecystolithiasis combined with choledocholithiasis: six years' experience of a single institution.

Surg Endosc 2021 Nov 3. Epub 2021 Nov 3.

Department of Biliary-Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, Hubei, China.

Introduction: The optimal treatment of choledocholithiasis combined with cholecystolithiasis remains controversial. Common surgical methods vary among endoscopic retrograde cholangiopancreatography (ERCP) followed by laparoscopic cholecystectomy (LC), laparoscopic transcystic common bile duct exploration (LTCBDE), laparoscopic transductal common bile duct exploration (LCBDE) with or without T-tube drainage. The purpose of this study is to evaluate the safety and effectiveness of surgical methods and to determine the appropriate procedure for patients with cholecystolithiasis combined with choledocholithiasis.

Methods: From January 2013 to January 2019, a total of 1555 consecutive patients diagnosed with cholecystolithiasis combined with choledocholithiasis who underwent surgical treatment in Tongji Hospital were retrospectively analyzed. Total 521 patients with intrahepatic bile duct stones underwent LC + LCBDE + T-Tube were excluded from the analysis. At last, 1034 patients who met the inclusion criteria were divided into three groups according to their surgical methods: preoperative ERCP + subsequent LC (ERCP + LC group, n = 275), LC + LCBDE + intraoperative endoscopic nasobiliary drainage (ENBD) + primary duct closure (Tri-scope group, n = 479) and LC + laparoscopic transcystic CBD exploration (LTCBDE group, n = 280). Clinical records, operative findings and postoperative follow-up were collected and analyzed.

Results: There was no mortality in three groups. Common bile duct (CBD) stone clearance rate was 97.5% in ERCP + LC group, 98.7% in Tri-scope group, and 99.3% in LTCBDE group. There were no difference in terms of demographic characteristics, biochemistry findings and presentations, but the Tri-scope group had the biggest diameter and amount of stones and diameter of CBD, the LTCBDE group had the least CBD stones and the biggest diameter of cystic gall duct (CGD). ERCP + LC group have the longest hospital stay (14.16 ± 3.88 days vs 6.92 ± 1.71 days vs 10.74 ± 5.30 days, P < 0.05), also has the longest operative time than others (126.08 ± 42.79 min vs 92.31 ± 10.26 min, 99.09 ± 8.46 min, P < 0.05). Compared to ERCP + LC group, LTCBDE group and Tri-scope group had lower postoperation-leukocyte, shorter surgery duration and hospital stay (P < 0.05). Compared to the Tri-scope group, the LTCBDE group had the shorter hospital stay, extubation time and operation time and less intraoperative bleeding. There were less postoperative complications in LTCBDE group (1.1%) compared to the ERCP + LC group (3.6%) and Tri-scope group (2.2%). Follow-up time was 6 to 72 months. Four patients in ERCP + LC group and 5 in Tri-scope group reported recurrent stones.

Conclusion: All the three surgical methods are safe and effective. Tri-scope approach and LTCBDE approach have superiority to preoperative ERCP + LC. LC + LTCBDE shows priority over Tri-scope approach, but should be performed in selected patients. LC + LCBDE + T-Tube can be an alternative management if the other three procedures were failed. The surgeons should choose the most appropriate surgical procedure according to the preoperative examination results and intraoperative situation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00464-021-08843-xDOI Listing
November 2021

Upregulation of GTPBP4 Promotes the Proliferation of Liver Cancer Cells.

J Oncol 2021 19;2021:1049104. Epub 2021 Oct 19.

Cancer Research Institute of Hengyang Medical College, University of South China, Hengyang, China.

Results: The GTPBP4 has upregulated expression in liver cancer patients ( < 0.01), but there was no difference in its expression in patients with different clinicopathological stages. The expression of GTPBP4 increased with the increase of cancer metastasis in lymph nodes ( < 0.01). Liver cancer patients with upregulated expression of GTPBP4 showed a shorter overall survival rate (=0.02). GTPBP4 is closely related to genes such as NIFK, WDR12, and RPF2, and these genes are involved in life processes such as GTP binding and rRNA processing. The upregulated expression of GTPBP4 promotes the proliferation of liver cancer cells and promotes the growth of tumors in mice, while the downregulated expression of GTPBP4 inhibits the proliferation of liver cancer cells and inhibits the growth of tumors in mice.

Conclusion: The expression of GTPBP4 is upregulated in liver cancer patients and affects the overall survival rate of patients. The upregulated expression of GTPBP4 promotes the proliferation of liver cancer cells and the growth of tumors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/1049104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548153PMC
October 2021

Stabilizing Fe-N-C Catalysts as Model for Oxygen Reduction Reaction.

Adv Sci (Weinh) 2021 Oct 23:e2102209. Epub 2021 Oct 23.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, P. R. China.

The highly efficient energy conversion of the polymer-electrolyte-membrane fuel cell (PEMFC) is extremely limited by the sluggish oxygen reduction reaction (ORR) kinetics and poor electrochemical stability of catalysts. Hitherto, to replace costly Pt-based catalysts, non-noble-metal ORR catalysts are developed, among which transition metal-heteroatoms-carbon (TM-H-C) materials present great potential for industrial applications due to their outstanding catalytic activity and low expense. However, their poor stability during testing in a two-electrode system and their high complexity have become a big barrier for commercial applications. Thus, herein, to simplify the research, the typical Fe-N-C material with the relatively simple constitution and structure, is selected as a model catalyst for TM-H-C to explore and improve the stability of such a kind of catalysts. Then, different types of active sites (centers) and coordination in Fe-N-C are systematically summarized and discussed, and the possible attenuation mechanism and strategies are analyzed. Finally, some challenges faced by such catalysts and their prospects are proposed to shed some light on the future development trend of TM-H-C materials for advanced ORR catalysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.202102209DOI Listing
October 2021

Long non-coding RNA SNHG17 promotes lung adenocarcinoma progression by targeting the microRNA-193a-5p/NETO2 axis.

Oncol Lett 2021 Dec 5;22(6):818. Epub 2021 Oct 5.

Department of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, P.R. China.

Long non-coding RNAs (lncRNAs) play vital roles in human cancers. It has been reported that lncRNA SNHG17 expression is dysregulated in different types of cancer and involved in cancer progression. However, the role of SNHG17 in lung adenocarcinoma (LUAD) remains unclear. The present study aimed to investigate the role of SNHG17 in LUAD. Reverse transcription-quantitative (RT-q) PCR analysis was performed to detect SNHG17 expression in LUAD tissues and cells. The effects of SNHG17 on cancer cell migration, invasion, proliferation and epithelial-to-mesenchymal transition (EMT) were assessed via Transwell, MTT and western blot assays, respectively. The interactions between SNHG17 and microRNA (miRNA/miR)-193a-5p, miR-193a-5p and neuropilin and tolloid-like 2 (NETO2) were assessed via the dual-luciferase reporter assay. NETO2 expression and its potential role in LUAD were analyzed via RT-qPCR analysis and the UALCAN database. The results demonstrated that SNHG17 expression was significantly upregulated in LUAD tissues and cells, and high SNHG17 expression was associated with tumor-node-metastasis stage and poor prognosis of patients with LUAD. SNHG17 knockdown inhibited cell migration, invasion, proliferation and the EMT process. In addition, the results revealed that SNHG17 functions as a competing endogenous RNA of miR-193a-5p. The results of the dual-luciferase reporter assay confirmed that miR-193a-5p can directly target SNHG17. NETO2 was also predicted as a target protein of miR-193a-5p, which was confirmed via the dual-luciferase reporter assay. The roles of NETO2 knockdown in cancer cells were rescued following transfection with miR-193a-5p inhibitor or overexpression of SNHG17. Notably, high NETO2 expression was associated with poor prognosis of patients with LUAD. Bioinformatics analysis demonstrated that the promoter methylation level of NETO2 decreased in LUAD. Taken together, the results of the present study suggest that SNHG17 expression is upregulated in LUAD tissues and cells, and SNHG17 exerts tumor promoting effect by targeting the miR-193a-5p/NETO2 axis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/ol.2021.13079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503812PMC
December 2021

High levels of mild to moderate depression among men who have sex with men and transgender women in Lima, Peru: implications for integrated depression and HIV care.

AIDS Care 2021 Oct 20:1-6. Epub 2021 Oct 20.

Department of Social Medicine, Population and Public Health, University of California Riverside School of Medicine, Riverside, United States.

Depression disproportionally affects people at risk of acquiring or living with HIV and is associated with worse health outcomes; however, depression care is not routinely integrated with HIV prevention and treatment services. Selection of the best depression intervention(s) for integration depends both on the prevalence and severity of depression among potential users. To inform depression care integration in a community-based setting in Lima, Peru, we retrospectively analyzed routinely collected depression screening data from men who have sex with men and transgender women seeking HIV prevention and care services (N = 185). Depression was screened for using the Patient Health Questionnaire-9. Prevalence of any depression (PHQ-9 ≥ 5) was 42% and was significantly associated with the last sexual partner being "casual" (= 0.01). Most (81%) depressive symptoms were mild to moderate (≥5 PHQ-9 ≤ 14). Integrating depression care with HIV prevention and treatment services in Peru should begin by implementing interventions targeting mild to moderate depression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/09540121.2021.1991877DOI Listing
October 2021

Changing Incidence and Mechanism of Pregnancy-Associated Myocardial Infarction in the State of California.

J Am Heart Assoc 2021 Nov 20;10(21):e021056. Epub 2021 Oct 20.

Department of Internal Medicine and Cardiology University of California Riverside School of Medicine Riverside CA.

Background The objective of this study was to evaluate the temporal trends in pregnancy-associated myocardial infarction (PAMI) in the State of California and explore potential risk factors and mechanisms. Methods and Results The California State Inpatient Database was analyzed from 2003 to 2011 for patients with () codes for acute myocardial infarction and pregnancy or postpartum admissions; risk factors were analyzed and compared with pregnant patients without myocardial infarction. A total of 341 patients were identified with PAMI from a total of 5 266 380 pregnancies (incidence of 6.5 per 100 000 pregnancies). Inpatient maternal mortality rate was 7%, and infant mortality rate was 3.5% among patients with PAMI. There was a nonsignificant trend toward an increase in PAMI incidence from 2003 to 2011, possibly attributable to higher incidence of spontaneous coronary artery dissection, vasospasm, and Takotsubo syndrome. PAMI, when compared with pregnant patients without myocardial infarction, was significant for older age (aged >30 years in 72% versus 37%, <0.0005), higher preponderance of Black race (12% versus 6%, <0.00005), lower socioeconomic status (median household income in lowest quartile 26% versus 20%, =0.04), higher prevalence of hypertension (26% versus 7%, <0.0005), diabetes (7% versus 1%, <0.0005), anemia (31% versus 7%, <0.0001), amphetamine use (1% versus 0%, <0.00005), cocaine use (2% versus 0.2%, <0.0001), and smoking (6% versus 1%, =0.0001). Conclusions There has been a trend toward an increase in PAMI incidence in California over the past decade, with an increasing trend in spontaneous coronary artery dissection, vasospasm, and Takotsubo syndrome as mechanisms. These findings warrant further investigation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1161/JAHA.121.021056DOI Listing
November 2021

Polyphyllin I, a lethal partner of Palbociclib, suppresses non-small cell lung cancer through activation of p21/CDK2/Rb pathway and .

Cell Cycle 2021 Oct 17:1-13. Epub 2021 Oct 17.

Department of Pathology, Yunnan University of Chinese Medicine, Kunming, Yunnan, China.

Cyclin-dependent kinases (CDKs) are hyperactive in many cancers and have served as cancer therapeutic targets for decades. Palbociclib (Palb) is the first approved CDK4/6 inhibitor to treat hormone receptor (HR)-positive, HER2-negative advanced breast cancer. Acquired drug resistance is one obstacle of Palb be utilized in other cancer. CDK2 compensation of CDK4/6 loss is one of the causes that cancer cells are resistant to Palb. Hence, targeting multiple CDKs could be a novel strategy to prevent the drug resistance of cancer cells and expand the application of Palb in other cancer. In this study, we initially indicated Polyphyllin I (PPI) significantly inhibits non-small lung cancer cell (NSCLC) proliferation, promotes cell apoptosis and . Mechanistically, PPI can inhibit Rb through the p21/CDK2/Rb signaling pathway in NSCLC. A combination of PPI and Palb exerts a significant synergistic anti-cancer ability on NSCLC. Of note, PPI can reverse Palb drug resistance. Herein, we first time demonstrated PPI can disturb CDK2 function through upregulation of p21. The PPI effect on CDK2 provides a choice for a chemotherapeutic strategy for the elimination of NSCLC. Our study highlighted the clinical significance of simultaneously blocking of CDK2 and CDK4/6 for NSCLC treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/15384101.2021.1991121DOI Listing
October 2021

Radiographic optimization of the lateral position of the knee joint aided by CT images and the maximum intensity projection technique.

J Orthop Surg Res 2021 Oct 9;16(1):581. Epub 2021 Oct 9.

Department of Radiology, the First Affiliated Hospital of Chongqing Medical University, Number 1, Youyi Road, Yuzhong District, Chongqing City, China.

Background: Standard lateral knee-joint X-ray images are crucial for the accurate diagnosis and treatment of many knee-joint-related conditions. However, it is difficult to obtain standard lateral knee-joint X-ray images in the current knee-joint lateral radiography position.

Purpose: To optimize the lateral position of knee joint for radiography aided by computed tomography (CT) images and the maximum intensity projection technique.

Materials And Methods: One hundred cases of anteroposterior and lateral radiographs of knee joints were included. Of these, 50 cases were for lateral radiography in conventional position, and the other 50 cases were for lateral radiography in optimized position. The optimized position was acquired by a retrospective analysis of one hundred cases of knee-joint CT images. The quality of the X-ray images in optimized group was compared with those in conventional group. The data were statistically analyzed using the Mann-Whitney U test.

Results: There were differences in the optimized position between males and females. The posterior condyles of the femoral epiphysis in optimized group were in perfect superimposition for most patients. However, the ones in conventional group were not. The average quality score of the lateral knee-joint X-ray images in optimized position was 3.76 ± 0.98, which is much higher than the 1.84 ± 1.15 score in conventional position. Moreover, the difference in the average quality score was statistically significant (P < 0.05).

Conclusion: Optimization of the lateral position of knee joint for radiography is possible with the aid of CT images and the maximum intensity projection technique.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-021-02740-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501547PMC
October 2021

Transcatheter Closure of Perimembranous and Intracristal Ventricular Septal Defects Using Amplatzer Duct Occluder II in Children.

J Interv Cardiol 2021 11;2021:4091888. Epub 2021 Sep 11.

Department of Cardiac Pediatrics, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangdong Provincial People's Hospital, Guangzhou 510080, China.

Background: Transcatheter closure of aneurysmal perimembranous ventricular septal defect (pmVSD), pmVSD near the aortic valve, and intracristal VSD (icVSD) with symmetrical or asymmetrical ventricular septal defect occluders still presents significant challenges. We report our experience with transcatheter closure of pmVSD and icVSD using Amplatzer duct occluder II (ADO II) in children.

Method: We retrospectively analyzed all children, who presented to our hospital consecutively between March 2014 and June 2020 for attempted transcatheter closure of pmVSD or icVSD with the ADO II device. Standard safety and last-follow-up outcomes were assessed and compared.

Results: In total, 41 patients underwent transcatheter closure of VSD with the ADO II (28 in pmVSD and 13 in icVSD groups) with a median age of 3.5 years (total range: 0.9 to 12 years) and median weight of 15.0 kg (total range: 10.0 to 43.0 kg). Implantation was successful in 40/41 patients (97.5%, 27/28 in pmVSD group, 13/13 in icVSD group). One patient with mild aortic valve prolapse in pmVSD group developed new-onset moderate aortic regurgitation after a 4/4 mm ADO II was deployed; however, this resolved after the device was retrieved and successfully replaced with a 5 mm zero eccentric VSD occluder. There was no procedure-related mortality. After a median follow-up of six months (total range: 6 to 72 months), complete closure rates were 85.1% and 76.9% among pmVSD and icVSD groups, respectively. In the pmVSD group, one case of new-onset moderate tricuspid regurgitation was observed at six months, and there was one case of severe tricuspid regurgitation that had progressed from mild tricuspid regurgitation at 12 months. No serious complications were noted in the icVSD group.

Conclusion: ADO II provides a safe and reproducible alternative for the closure of perimembranous and intracristal ventricular septal defects with a diameter less than 5 mm in young children.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/4091888DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452420PMC
November 2021

Borylative Cyclization of 1,6-Allenynes Driven by BCl.

Org Lett 2021 Oct 6;23(20):8050-8055. Epub 2021 Oct 6.

Henan Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, 436 Xian'ge Road, Anyang 455000, People's Republic of China.

A metal-free intramolecular borylative cyclization of 1,6-allenynes driven by BCl was developed. This method provides a general and practical strategy to construct valuable pyrrolidines containing all-carbon quaternary centers or 3,5-dihydroazepine derivatives depending on the substituents of the allene, with conjugative and sterically hindered phenyl groups favoring the latter.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.orglett.1c03062DOI Listing
October 2021

Association of the low-carbohydrate dietary pattern with postpartum weight retention in women.

Food Funct 2021 Nov 1;12(21):10764-10772. Epub 2021 Nov 1.

Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, Guangdong, P. R. China.

Low-carbohydrate diets (LCD) have been considered a popular dietary strategy for weight loss. However, the association of the low-carbohydrate dietary pattern with postpartum weight retention (PPWR) in women remains unknown. The present study involved 426 women from a prospective mother-infant cohort study. Overall, animal or plant LCD scores, which represent adherence to different low-carbohydrate dietary patterns, were calculated using diet intake information assessed by three consecutive 24 h dietary surveys. PPWR was assessed by the difference of weight at 1 year postpartum minus the pre-pregnancy weight. After adjusting for potential confounding variables, women in higher quartiles of total and animal-based LCD scores had a significantly lower body weight and weight retention at 1 year postpartum ( < 0.05). The multivariable-adjusted ORs of substantial PPWR (≥5 kg), comparing the highest with the lowest quartile, were 0.47 (95% confidence interval 0.23-0.96) for the total LCD score ( = 0.021 for trend) and 0.38 (95% confidence interval 0.19-0.77) for the animal-based LCD score ( = 0.019 for trend), while this association was significantly attenuated by rice, glycemic load, fish, poultry, animal fat and animal protein ( for trend <0.05). A high score for plant-based LCD was not significantly associated with the risk of PPWR ( > 0.05). The findings suggested that a low-carbohydrate dietary pattern, particularly with high protein and fat intake from animal-source foods, is associated with a decreased risk of weight retention at 1 year postpartum. This association was mainly due to low intake of glycemic load and high intake of fish and poultry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1fo00935dDOI Listing
November 2021

Comparing the Interface pressure distribution of the full body chest-lumbar cushion made of memory cotton with the traditional chest cushion.

BMC Musculoskelet Disord 2021 Sep 30;22(1):839. Epub 2021 Sep 30.

Department of Operating Room, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Background: Pressure injuries are common complications occurred duration hospitalization, whether the interface pressure distribution in full body memory cotton chest-lumbar cushion was superior than traditional chest cushion remains unclear.

Purpose: This study aimed to compare the effects that the full body memory cotton chest-lumbar cushion versus traditional chest cushion on interface pressure.

Methods: A total of 66 healthy individuals were recruited. The volunteers were placed in the left lateral position and left armpit and iliac spine pressure and level of comfort were measured. Group differences were assessed using the paired t-test or Wilcoxon test according to data distribution. Additionally, multivariate regression analysis was applied to determine the potential role of sex, age, and body mass index on left armpit and iliac spine pressure and overall comfort.

Results: Compared with the traditional chest cushion, we noted that the full body chest-lumbar cushion made of memory cotton was associated with less pressure on the left armpit (38.17 ± 10.39 mmHg vs. 67.93 ± 14.67 mmHg, respectively; P < 0.0001) and iliac spine (43.32 ± 13.70 mmHg vs. 50.77 ± 20.94 mmHg, respectively; P = 0.0004). Moreover, we noted that the overall comfort with the memory cotton chest-lumbar cushion was higher than that with the traditional chest cushion (8.48 ± 1.08 vs. 6.36 ± 1.45, respectively; P < 0.0001). Finally, the multivariate regression analyses found iliac spine pressure could affect by sex (P = 0.0377) and body mass index (P = 0.0380).

Conclusions: The full body chest-lumbar cushion made of memory cotton had beneficial effects on left armpit and iliac spine pressure and on comfort. These findings should be applied to future clinical practice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12891-021-04668-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8485509PMC
September 2021

Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid.

BMC Biotechnol 2021 09 25;21(1):55. Epub 2021 Sep 25.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, 368 Youyi Road, Wuchang, Wuhan, 430062, China.

Background: The unnatural amino acid, L-2-aminobutyric acid (L-ABA) is an essential chiral building block for various pharmaceutical drugs, such as the antiepileptic drug levetiracetam and the antituberculosis drug ethambutol. The present study aims at obtaining variants of ω-transaminase from Ochrobactrum anthropi (OATA) with high catalytic activity to α-ketobutyric acid through protein engineering.

Results: Based on the docking model using α-ketobutyric acid as the ligand, 6 amino acid residues, consisting of Y20, L57, W58, G229, A230 and M419, were chosen for saturation mutagenesis. The results indicated that L57C, M419I, and A230S substitutions demonstrated the highest elevation of enzymatic activity among 114 variants. Subsequently, double substitutions combining L57C and M419I caused a further increase of the catalytic efficiency to 3.2-fold. This variant was applied for threonine deaminase/OATA coupled reaction in a 50-mL reaction system with 300 mM L-threonine as the substrate. The reaction was finished in 12 h and the conversion efficiency of L-threonine into L-ABA was 94%. The purity of L-ABA is 75%, > 99% ee. The yield of L-ABA was 1.15 g.

Conclusion: This study provides a basis for further engineering of ω-transaminase for producing chiral amines from keto acids substrates.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12896-021-00713-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466713PMC
September 2021

Novel zebrafish polycystic kidney disease models reveal functions of the Hippo pathway in renal cystogenesis.

Dis Model Mech 2021 Nov 9;14(11). Epub 2021 Nov 9.

Department of Biomedical Sciences and Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau 999078, China.

The Hippo signaling pathway is a kinase cascade that plays an important role in organ size control. As the main effectors of the Hippo pathway, transcription coactivators Yap1/Wwtr1 are regulated by the upstream kinase Stk3. Recent studies in mammals have implicated the Hippo pathway in kidney development and kidney diseases. To further illustrate its roles in vertebrate kidney, we generated a series of zebrafish mutants targeting stk3, yap1 and wwtr1 genes. The stk3-/- mutant exhibited edema, formation of glomerular cysts and pronephric tubule dilation during the larval stage. Interestingly, disruption of wwtr1, but not yap1, significantly alleviated the renal phenotypes of the stk3-/- mutant, and overexpression of Wwtr1 with the CMV promoter also induced pronephric phenotypes, similar to those of the stk3-/- mutant, during larval stage. Notably, adult fish with Wwtr1 overexpression developed phenotypes similar to those of human polycystic kidney disease (PKD). Overall, our analyses revealed roles of Stk3 and Wwtr1 in renal cyst formation. Using a pharmacological approach, we further demonstrated that Stk3-deficient zebrafish could serve as a PKD model for drug development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1242/dmm.049027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592019PMC
November 2021

circRNA-miRNA-mRNA in breast cancer.

Clin Chim Acta 2021 Sep 16;523:120-130. Epub 2021 Sep 16.

Cancer Research Institute of Hengyang Medical College, University of South China, Key Laboratory of Cancer Cellular and Molecular Pathology in Hunan Province, Hunan, Hengyang 421001, China.

Among cancers, breast cancer has the highest incidence rate among women and poses a tremendous threat to women's health. Messenger RNA (mRNA), microRNA (miRNA) and circular RNA (circRNA) play vital roles in the progression of breast cancer through a variety of biological effects and mechanisms. Recently, the regulatory network formed by circRNAs, miRNAs and mRNAs has piqued attention and garnered interest. CircRNAs bind to miRNAs through a regulatory mechanism in which endogenous RNAs compete to indirectly regulate the expression of mRNA corresponding to downstream target genes of miRNAs, contributing to the progression of breast cancer. The circRNA-miRNA-mRNA axis may be a marker for the early diagnosis and prognosis of breast cancer and a potential breast cancer treatment target, providing unlimited possibilities for the development of breast cancer biomarkers and therapeutic strategies. This article reviews recent research progress on the circRNA-miRNA-mRNA axis as a regulatory network of competing endogenous RNAs in breast cancer. Herein, we focus on the mechanism and function of the circRNA-miRNA-mRNA axis in the occurrence and metastasis of breast cancer, and resistance to chemotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cca.2021.09.013DOI Listing
September 2021

Mediator complex proximal Tail subunit MED30 is critical for Mediator core stability and cardiomyocyte transcriptional network.

PLoS Genet 2021 09 10;17(9):e1009785. Epub 2021 Sep 10.

Department of Medicine, University of California, San Diego, California, United States of America.

Dysregulation of cardiac transcription programs has been identified in patients and families with heart failure, as well as those with morphological and functional forms of congenital heart defects. Mediator is a multi-subunit complex that plays a central role in transcription initiation by integrating regulatory signals from gene-specific transcriptional activators to RNA polymerase II (Pol II). Recently, Mediator subunit 30 (MED30), a metazoan specific Mediator subunit, has been associated with Langer-Giedion syndrome (LGS) Type II and Cornelia de Lange syndrome-4 (CDLS4), characterized by several abnormalities including congenital heart defects. A point mutation in MED30 has been identified in mouse and is associated with mitochondrial cardiomyopathy. Very recent structural analyses of Mediator revealed that MED30 localizes to the proximal Tail, anchoring Head and Tail modules, thus potentially influencing stability of the Mediator core. However, in vivo cellular and physiological roles of MED30 in maintaining Mediator core integrity remain to be tested. Here, we report that deletion of MED30 in embryonic or adult cardiomyocytes caused rapid development of cardiac defects and lethality. Importantly, cardiomyocyte specific ablation of MED30 destabilized Mediator core subunits, while the kinase module was preserved, demonstrating an essential role of MED30 in stability of the overall Mediator complex. RNAseq analyses of constitutive cardiomyocyte specific Med30 knockout (cKO) embryonic hearts and inducible cardiomyocyte specific Med30 knockout (icKO) adult cardiomyocytes further revealed critical transcription networks in cardiomyocytes controlled by Mediator. Taken together, our results demonstrated that MED30 is essential for Mediator stability and transcriptional networks in both developing and adult cardiomyocytes. Our results affirm the key role of proximal Tail modular subunits in maintaining core Mediator stability in vivo.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1371/journal.pgen.1009785DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8432849PMC
September 2021

Nomimicins B-D, new tetronate-class polyketides from a marine-derived actinomycete of the genus .

Beilstein J Org Chem 2021 27;17:2194-2202. Epub 2021 Aug 27.

Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.

Three new tetronate-class polyketides, nomimicins B, C, and D, along with nomimicin, hereafter named nomimicin A, were isolated from the culture extract of sp AKA43 collected from floating particles in the deep-sea water of Sagami Bay, Japan. The structures of nomimicins B, C, and D were elucidated through the interpretation of NMR and MS analytical data, and the absolute configuration was determined by combination of NOESY/ROESY and ECD analyses. Nomimicins B, C, and D showed antimicrobial activity against Gram-positive bacteria, and , with MIC values in the range of 6.5 to 12.5 μg/mL Nomimicins B and C also displayed cytotoxicity against P388 murine leukemia cells with IC values of 33 and 89 μM, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3762/bjoc.17.141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404215PMC
August 2021

Associations of dietary inflammatory potential with postpartum weight change and retention: Results from a cohort study.

Obesity (Silver Spring) 2021 Oct 7;29(10):1689-1699. Epub 2021 Sep 7.

Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Nutrition and Food Hygiene, School of Public Health, Southern Medical University, Guangzhou, People's Republic of China.

Objective: This study examined the associations of Dietary Inflammatory Index (DII) with weight outcomes within 1 year post partum.

Methods: This analysis included women who participated in a cohort study in South China (n = 468). The assessments included maternal height, weight, and dietary intake. The latter variable was based on three consecutive 24-hour food records collected at 2 weeks and 1 year after childbirth and was used to calculate the energy-adjusted DII (EDII) scores during and after puerperium, respectively. A general linear regression was performed to examine the relationships between the EDII scores and postpartum weight outcomes after adjusting for confounders.

Results: In an analysis adjusted for confounders, the EDII during puerperium was positively associated with the weight change from 3 to 42 days (β: 0.42, 95% CI: 0.11-0.70). The EDII after puerperium was positively correlated with the weight changes from 42 days to 1 year (β: 0.52, 95% CI: 0.02-1.02) and from 3 days to 1 year (β: 0.63, 95% CI: 0.13-1.14), as well as with the postpartum weight retention at 1 year after childbirth (β: 0.75, 95% CI: 0.29-1.22).

Conclusions: The results indicate that a diet with a high EDII score might minimize postpartum weight loss and promote higher postpartum weight retention.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/oby.23238DOI Listing
October 2021

Down-regulated placental miR-21 contributes to preeclampsia through targeting RASA1.

Hypertens Pregnancy 2021 Aug 31;40(3):236-245. Epub 2021 Aug 31.

Department of Obstetrics, First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong, China.

Human placenta was obtained from early onset preeclampsia, late onset preeclampsia, and their gestational age-matched normal pregnancy. Using RT-qPCR, western blot, and immunohistochemistry, it was demonstrated that miR-21 expressions were significantly decreased in preeclampsia while RASA1 were increased. Suppression of miR-21 in placental HTR-8/SVneo cells, remarkably upregulated RASA1, decreased proliferation, inhibited invasion, and promoted apoptosis of trophoblast cells, while overexpression of miR-21 alleviated these effects. Dual-luciferase reporter assays revealed RASA1 to be a direct target of miR-21 in trophoblast cells. miR-21 may serve key roles in the development of preeclampsia by targeting RASA1.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10641955.2021.1974031DOI Listing
August 2021

Long-term clinical observation of patients with acute and chronic complete spinal cord injury after transplantation of NeuroRegen scaffold.

Sci China Life Sci 2021 Aug 16. Epub 2021 Aug 16.

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, China.

Spinal cord injury (SCI) often results in an inhibitory environment at the injury site. In our previous studies, transplantation of a scaffold combined with stem cells was proven to induce neural regeneration in animal models of complete SCI. Based on these preclinical studies, collagen scaffolds loaded with the patients' own bone marrow mononuclear cells or human umbilical cord mesenchymal stem cells were transplanted into SCI patients. Fifteen patients with acute complete SCI and 51 patients with chronic complete SCI were enrolled and followed up for 2 to 5 years. No serious adverse events related to functional scaffold transplantation were observed. Among the patients with acute SCI, five patients achieved expansion of their sensory positions and six patients recovered sensation in the bowel or bladder. Additionally, four patients regained voluntary walking ability accompanied by reconnection of neural signal transduction. Among patients with chronic SCI, 16 patients achieved expansion of their sensation level and 30 patients experienced enhanced reflexive defecation sensation or increased skin sweating below the injury site. Nearly half of the patients with chronic cervical SCI developed enhanced finger activity. These long-term follow-up results suggest that functional scaffold transplantation may represent a feasible treatment for patients with complete SCI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11427-021-1985-5DOI Listing
August 2021

Initial Clinical Experience with the Biodegradable Absnow Device for Percutaneous Closure of Atrial Septal Defect: A 3-Year Follow-Up.

J Interv Cardiol 2021 30;2021:6369493. Epub 2021 Jul 30.

Department of Pediatric Cardiology, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangzhou 510100, China.

Objective: We reported the 3-year follow-up results of initial clinical experience with the Absnow device, a novel biodegradable occluder for percutaneous closure of atrial septal defect (ASD).

Background: The Absnow device is a total biodegradable septal occluder with double-disc poly-L-lactic acid (PLLA) framework and PLLA membranes intergraded into the device to ensure its biodegradability, clinical safety, and efficacy.

Methods: Five pediatric patients were enrolled from May to June 2018 in our institution and were followed up for 3 years. A clinical evaluation and transthoracic echocardiography were performed at 24 hr, 1 month, 3 months, 6 months, 12 months, and yearly after implantation. Primary endpoints were a composite clinical success, comprising of clinical closure success and safety at the 36-month follow-up evaluation. Secondary endpoints included technical success, procedure success, closure success, and safety at each of the follow-up visits.

Results: The median subject age was 3.6 years (range 3.1-6.5 years). The mean ASD diameter was (13.7 ± 2.9) mm. The median device size was 20 mm (range 14 to 24 mm). Technical and procedure success was achieved in 100% (5/5) of the patients. At 2-year follow-up, 3 of the 5 patients developed new-onset residual shunts and 2 of them reached a moderate degree. At 3-year follow-up, the residual shunt size increased over time in all the 3 patients, and 1 of them had right ventricular enlargement. All of the 5 patients were free from serious adverse events during the 3-year follow-up, with no device embolization, thromboembolization, or reintervention to the target defect.

Conclusion: This 3-year follow-up result of initial experience with the biodegradable Absnow device has demonstrated acceptable safety with no procedural complications. Notably, the high rate of residual shunt significantly affected its efficacy. The long-term safety and efficacy of the device should be further evaluated in a large cohort of patients in future studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6369493DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349294PMC
October 2021

MicroRNA and cyclooxygenase-2 in breast cancer.

Clin Chim Acta 2021 Nov 10;522:36-44. Epub 2021 Aug 10.

Department of Anesthesiology, Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421002, PR China.

Cancer remains a major public health problem worldwide and the latest statistics show that breast cancer (BC) is among the most frequent in women. MicroRNAs (miRNAs; miRs) and cyclooxygenase-2 (COX-2) are new diagnostic and therapeutic biomarkers for monitoring BC. COX-2 is a prominent tumor-associated inflammatory factor highly expressed in human tumor cells, including BC. Expression of COX-2 contributes to tumor growth, metastasis and recurrence. MiRs are a group of short (~22 nucleotides), noncoding regulatory RNAs that downregulate gene expression post-transcriptionally and play vital roles in regulating cancer development and progression. Interestingly, there are a group of miRNAs differentially expressed in breast tumor tissue. Understanding the pathway linking miRNAs to COX-2 can provide novel insight for suppressing COX-2 expression via gene silencing thereby leading to the development of selective miRNA inhibitors. Further research can also reveal key intermediate players and their potential as therapeutic targets. Given the association between different miRNAs and COX-2 expression in BC, this review presents a comprehensive overview of the current literature concerning how miRNAs and COX-2 signaling interact in BC progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cca.2021.08.007DOI Listing
November 2021

Relationship between ultrasound values and pathology and metastasis in patients with breast cancer.

Am J Transl Res 2021 15;13(7):8207-8213. Epub 2021 Jul 15.

Galactophore Department, Hangzhou Fuyang Women and Children Hospital Hangzhou 311400, Zhejiang, China.

Purpose: This study was designed to investigate the relationship between ultrasound values of breast cancer and its pathology and metastasis.

Methods: A retrospective study was conducted on 80 patients diagnosed with breast cancer by pathologic examination in our hospital. The tumor size, tumor type, tumor grade, and the presence of distant metastasis were recorded. Vascular invasion, molecular subtype, pathobiologic indicators, and other measures were analyzed to explore the correlation between ultrasound measurements and pathology and metastasis in breast cancer patients.

Result: The proportion of ultrasound scores did not differ significantly among the groups ( > 0.05). The enrolled subjects were grouped according to tumor types (intraductal carcinoma, invasive ductal carcinoma, invasive lobular carcinoma, and special types), tumor grade (grade 1-3), metastasis, vascular invasion, and pathobiologic indicators (positive or negative ER/PR and HER-2 expression). These factors affected the ultrasound scores of breast cancer patients, resulting in significant differences in the proportions of scores between the groups ( < 0.05).

Conclusion: The ultrasound scores of breast cancer are closely related to its pathologic changes, and this has implications for the types of pathological tissues, biologic indicators, and presence of metastasis. Therefore, ultrasound values may be useful as a primary pathologic screening method for breast cancer patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340147PMC
July 2021

Analysis of the influence of a solenoid magnetic field in the azimuth transmission system.

Sci Rep 2021 Aug 10;11(1):16242. Epub 2021 Aug 10.

Xi'an Research Institute of High-Tech, Xi'an, 710000, Shaanxi, China.

A solenoid magnetic field plays an important role in a non-line-of-sight azimuth transmission system based on polarization-maintaining fiber, which is directly related to the transmission accuracy of azimuth information. This research mainly studies the factors that affect the solenoid magnetic field according to the modulation signal from the direct current to the alternating current, as well as the hollow solenoid. First, the magnetic field components of the static solenoid are derived from the Biot-Savart law by using the uniform cylindrical current equivalent model. Then, the magnetic field of the near axial region is studied from the axial and radial directions, and the feasibility of calculating the magnetic field of the multi-layer solenoid with the superposition principle is verified by measuring the magnetic field of each position on the axis of the solenoid with a Gauss meter. Finally, the alternating electromagnetic field model is established using Maxwell's equations, and the magnetic and electric fields of the hollow solenoid are further solved. The results show that the magnetic field in the middle part of the magneto-optic glass is more stable, and the magnetic collecting ability of the solenoid is stronger. The magnetic field intensity at the center of the magneto-optic modulation solenoid of the system is the largest, and it decreases with the distance from the center. The alternating electromagnetic field is closely related to frequency. The results provide a reference for the study of the azimuth accuracy of a non-line-of-sight azimuth transmission system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-95783-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355376PMC
August 2021

Closing-wedge distal femoral osteotomy combined with medial patellofemoral ligament reconstruction for recurrent patellar dislocation with genu valgum.

BMC Musculoskelet Disord 2021 Aug 9;22(1):668. Epub 2021 Aug 9.

Department of Orthopedics, Affiliated Hospital to Shandong University of Traditional Chinese Medicine, Jinan, 250011, China.

Background: Medial patellofemoral ligament reconstruction (MPFLR) is a well-established procedure for addressing recurrent patellar dislocation (RPD) in young patients. However, despite being a promising procedure for RPD with genu valgum, there is a scarcity of reports on simultaneous MPFLR and closing-wedge distal femoral osteotomy (CWDFO). The purpose of the present study was to observe and analyse the clinical and imaging findings of CWDFO combined with MPFLR for RPD with genu valgum.

Methods: From May 2015 to April 2018, 25 patients with RPD and genu valgum were surgically treated in our department. Anteroposterior long-leg, weight-bearing, lower-extremity radiographs, lateral radiographs and computed tomography (CT) scans of the patellofemoral joint were obtained, and the anatomical femorotibial angle (aFTA), mechanical lateral distal femoral angle (mLDFA), weight-bearing line rate (WBLR), patellar height, patellar lateral shift (PLS) and tibial tubercle-trochlear groove (TT-TG) distance were analysed. Validated knee scores, such as the Kujala, Lysholm, visual analogue scale (VAS) scores and Tegner socres, were evaluated preoperatively and 2 years postoperatively.

Results: 25 patients, with an average age of 19.8 years (14-27), were evaluated. During the 2-year follow-up period, all patients were able to achieve a better sports level without any problems, with no recurrence of patellar instability. Compared with preoperation, the aFTA, mLDFA, WBLR and PLS showed statistically significant improvement following the procedure (p < 0.001). Meanwhile, no significant differences in the Insall index and TT-TG distance were found. The mean Kujala score, average Lysholm score, VAS score and Tegner socres showed significant postoperative improvement.

Conclusions: CWDFO combined with MPFLR is a suitable treatment for RPD with genu valgum, and can lead to significant improvement in the clinical and imaging findings of the knee in the short term.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12891-021-04554-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351451PMC
August 2021
-->