Publications by authors named "Zhiwei Wang"

844 Publications

Circular RNA expression profile in transgenic diabetic mouse kidneys.

Cell Mol Biol Lett 2021 Jun 7;26(1):25. Epub 2021 Jun 7.

Laboratory Medicine Centre, The Affiliated Shunde Hospital of Guangzhou Medical University, Foshan, 528315, China.

Background: Diabetic nephropathy is one of the most important complications in patients with diabetes. The etiology and pathogenesis of diabetic nephropathy remain unclear. Several studies have indicated that circular RNAs (circRNAs) play crucial regulatory roles in numerous human diseases and normal physiology; however, to date, no study has focused on the comprehensive expression profile of circRNAs in the kidneys of diabetic mice. Therefore, we aimed to identify differentially expressed circRNAs in diabetic mouse kidneys to explore the possible roles of dysregulated circRNAs in diabetic nephropathy development.

Results: Diabetic BKS-Lepr/Nju (BKS-DB/Nju) mice and their nondiabetic wild-type littermates of C57BL/KsJ wild-type (WT) mice were used as experimental animals. Among all circRNAs identified by high-throughput RNA sequencing, four circRNAs were upregulated and ten were downregulated in diabetic mouse kidneys compared to those in nondiabetic mouse kidneys. After verification using quantitative reverse transcriptase polymerase chain reaction assays, we found that circR_1084, circR_182, circR_4, circR_50, circR_596, circR_897, and circR_203 were downregulated, whereas circR_627, circR_628, circR_735, and circR_801 were upregulated in the renal tissues of 8- and 16-week-old BKS-DB/Nju mice compared to those in WT mice.

Conclusion: We studied the circRNA expression profile in the kidneys of diabetic mice. Differentially expressed circRNAs may be useful as candidate biomarkers for diabetic nephropathy. Collectively, our results provide a novel theoretical basis for further investigation of the regulatory roles of circRNA in the etiology and pathogenesis of diabetic nephropathy.
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http://dx.doi.org/10.1186/s11658-021-00270-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182942PMC
June 2021

State-of-the-art management technologies of dissolved methane in anaerobically-treated low-strength wastewaters: A review.

Water Res 2021 May 19;200:117269. Epub 2021 May 19.

Department of Civil Engineering, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z4. Electronic address:

The recent advancement in low temperature anaerobic processes shows a great promise for realizing low-energy-cost, sustainable mainstream wastewater treatment. However, the considerable loss of the dissolved methane from anaerobically-treated low-strength wastewater significantly compromises the energy potential of the anaerobic processes and poses an environmental risk. In this review, the promises and challenges of existing and emerging technologies for dissolved methane management are examined: its removal, recovery, and on-site reuse. It begins by describing the working principles of gas-stripping and biological oxidation for methane removal, membrane contactors and vacuum degassers for methane recovery, and on-site biological conversion of dissolved methane into electricity or value-added biochemicals as direct energy sources or energy-compensating substances. A comparative assessment of these technologies in the three categories is presented based on methane treating efficiency, energy-production potential, applicability, and scalability. Finally, current research needs and future perspectives are highlighted to advance the future development of an economically and technically sustainable methane-management technology.
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http://dx.doi.org/10.1016/j.watres.2021.117269DOI Listing
May 2021

Improved Identification of Small Open Reading Frames Encoded Peptides by Top-Down Proteomic Approaches and De Novo Sequencing.

Int J Mol Sci 2021 May 22;22(11). Epub 2021 May 22.

Hubei Key Lab of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, No. 152 Luoyu Road, Wuhan 430079, China.

Small open reading frames (sORFs) have translational potential to produce peptides that play essential roles in various biological processes. Nevertheless, many sORF-encoded peptides (SEPs) are still on the prediction level. Here, we construct a strategy to analyze SEPs by combining top-down and de novo sequencing to improve SEP identification and sequence coverage. With de novo sequencing, we identified 1682 peptides mapping to 2544 human sORFs, which were all first characterized in this work. Two-thirds of these new sORFs have reading frame shifts and use a non-ATG start codon. The top-down approach identified 241 human SEPs, with high sequence coverage. The average length of the peptides from the bottom-up database search was 19 amino acids (AA); from de novo sequencing, it was 9 AA; and from the top-down approach, it was 25 AA. The longer peptide positively boosts the sequence coverage, more efficiently distinguishing SEPs from the known gene coding sequence. Top-down has the advantage of identifying peptides with sequential K/R or high K/R content, which is unfavorable in the bottom-up approach. Our method can explore new coding sORFs and obtain highly accurate sequences of their SEPs, which can also benefit future function research.
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http://dx.doi.org/10.3390/ijms22115476DOI Listing
May 2021

Succinylated ferritin as a novel nanocage-like vehicle of polyphenol: Structure, stability, and absorption analysis.

Food Chem 2021 May 12;361:130069. Epub 2021 May 12.

State Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science & Technology, Tianjin 300457, China. Electronic address:

Ferritin, a protein with an 8-nm cage structure, can encapsulate and deliver bioactive molecules. In this study, succinylation was adopted to modify plant ferritin to fabricate succinylated red been ferritin (SRBF) at pH 8.0. The SRBF was retained as a cage-like shape (12 nm diameter), while its secondary structure was altered, rendering higher negative charge accompanies by decreased surface hydrophobicity. The SRBF also demonstrated favorable property of reversible assembly regulated by pH-transitions (pH 2.0/7.0), thus enabled successful encapsulation of epigallocatechin gallate (EGCG) for fabrication of EGCG-loaded SRBF complexes with a diameter of ~12 nm. Succinylation enhanced the thermal stabilities of ferritin and the embedded EGCG. Moreover, SRBF markedly improved the transport efficiency of EGCG in Caco-2 monolayers relative to EGCG and that encapsulated in unmodified ferritin. These findings have extended the succinylation reaction for the cage-like protein modification, and facilitated the usage of ferritin variant in delivery of bioactive molecules.
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http://dx.doi.org/10.1016/j.foodchem.2021.130069DOI Listing
May 2021

Application of the Tissue-Engineered Plant Scaffold as a Vascular Patch.

ACS Omega 2021 May 23;6(17):11595-11601. Epub 2021 Apr 23.

School of Material Science and Engineering & Henan Key Laboratory of Advanced Magnesium Alloy & Key Laboratory of Materials Processing and Mold Technology (Ministry of Education), Zhengzhou University, Zhengzhou, Henan 450001, China.

Tissue-engineered plant scaffolds have shown promising applications in in vitro studies. To assess the applicability of natural plant scaffolds as vascular patches, we tested decellularized leaf and onion cellulose in a rat inferior vena cava patch venoplasty model. The leaf was decellularized, and the scaffold was loaded with polylactic--glycolic acid (PLGA)-based rapamycin nanoparticles (nanoparticles). Nanoparticle-perfused leaves showed decreased neointimal thickness after implantation on day 14; there were also fewer CD68-positive cells and PCNA-positive cells in the neointima in the nanoparticle-perfused patches than in the control patches. Onion cellulose was decellularized, coated with rapamycin nanoparticles, and implanted in the rat; the nanoparticle-coated onion cellulose patches also showed decreased neointimal thickness. These data show that natural plant-based scaffolds may be used as novel scaffolds for tissue-engineered vascular patches. However, further modifications are needed to enhance patch strength for artery implantations.
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http://dx.doi.org/10.1021/acsomega.1c00804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154004PMC
May 2021

UPLC-MS-Based Serum Metabolomics Reveals Potential Biomarkers of Ang II-Induced Hypertension in Mice.

Front Cardiovasc Med 2021 5;8:683859. Epub 2021 May 5.

Laboratory of Cardiovascular Research, School of Medicine, Jiangnan University, Wuxi, China.

Hypertension is caused by polygenic inheritance and the interaction of various environmental factors. Abnormal function of the renin-angiotensin-aldosterone system (RAAS) is closely associated with changes in blood pressure. As an essential factor in the RAAS, angiotensin II (Ang II) contributes to vasoconstriction and inflammatory responses. However, the effects of overproduction of Ang II on the whole body-metabolism have been unclear. In this study, we established a hypertensive mouse model by micro-osmotic pump perfusion of Ang II, and the maximum systolic blood pressure reached 140 mmHg after 2 weeks. By ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, the metabolites in the serum of hypertensive model and control mice were analyzed. Partial least squares discriminant analysis (PLS-DA) in both positive and negative ionization modes showed clear separation of the two groups. Perfusion of Ang II induced perturbations of multiple metabolic pathways in mice, such as steroid hormone biosynthesis and galactose metabolism. Tandem mass spectrometry revealed 40 metabolite markers with potential diagnostic value for hypertension. Our data indicate that non-targeted metabolomics can reveal biochemical pathways associated with Ang II-induced hypertension. Although researches about the clinical use of these metabolites as potential biomarkers in hypertension is still needed, the current study improves the understanding of systemic metabolic response to sustained release of Ang II in hypertensive mice, providing a new panel of biomarkers that may be used to predict blood pressure fluctuations in the early stages of hypertension.
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http://dx.doi.org/10.3389/fcvm.2021.683859DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131677PMC
May 2021

Nitrogen-doped lignin-derived biochar with enriched loading of CeO nanoparticles for highly efficient and rapid phosphate capture.

Int J Biol Macromol 2021 May 18;182:1484-1494. Epub 2021 May 18.

Liaoning Key Laboratory of Lignocellulosic Chemistry and Biomaterials, College of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, China. Electronic address:

Development of lignin-derived carbon adsorbents with ultrahigh phosphate adsorption activity and rapid adsorption kinetics is of great importance, yet limited success has been achieved. Herein, we develop a CeO functionalized N-doped lignin-derived biochar ([email protected]) via a cooperative modification strategy for effective and fast phosphate capture. The novel modification strategy not only contributes greatly to the loading of well-dispersed CeO nanoparticles with a smaller size, but also significantly increases the relative concentration of Ce(III) species on [email protected] Consequently, an enhanced capture capacity for phosphate (196.85 mg g) as well as extremely rapid adsorption kinetics were achieved in a wide operating pH range (2-10). Interestingly, [email protected] exhibited a strong phosphate adsorption activity at even low-concentration phosphorus-containing water. The removal efficiency and final P concentration reached 99.87% and 2.59 μg P L within 1 min at the phosphate concentration of 2 mg P L. Experiments and characterization indicated that Ce(III) species plays a predominant role for the phosphate capture, and ligand exchange, together with electrostatic attraction, are the main adsorption mechanism. This work develops not only an efficient carbon-based adsorbent for phosphate capture, but also promotes the high-value application of industrial lignin.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.109DOI Listing
May 2021

Fecal Microbiota Transplantation as an Effective Treatment for Carbapenem-Resistant Infection in a Renal Transplant Patient.

Infect Drug Resist 2021 14;14:1805-1811. Epub 2021 May 14.

Department of Urology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Henan University People's Hospital, Zhengzhou, People's Republic of China.

Background: In renal transplant recipients, carbapenem-resistant (CRKP) infection is a common complication, and usually associated with severe clinical outcomes due to a lack of effective treatment.

Case Presentation: A 37-year-old woman with CRKP infection one month after kidney transplantation was involved in this study. Microbial characteristics of fecal samples from the patient were analyzed. Fecal microbiota transplantation (FMT) was performed for treating the CRKP infection. One week after FMT, the patient's urine and anal swab cultures returned negative for CRKP, and 17 days after FMT, the incision showed complete healing. Moreover, the patient had no symptoms of infection two months after FMT. Alpha diversity analyses showed that before FMT, the patient was associated with obviously lower species richness and diversity than the donor, which significantly increased at one week, three weeks and two months after FMT. Beta diversity analyses showed that though the patient's microbial community post-FMT still differed from the donor composition, their distances decreased visibly, especially at one week and three weeks after FMT. Obvious shift in microbial composition could be observed before and after FMT. The microbial composition of the patient post FMT resembled the donor composition. Relative abundance of genera such as and increased after FMT, while the relative abundance of significantly decreased.

Conclusion: This study demonstrated the therapeutic effect of FMT on infections caused by CRKP for a renal transplant patient. Further studies are required to confirm the findings of this study.
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http://dx.doi.org/10.2147/IDR.S308308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131010PMC
May 2021

Identification and elimination of cancer cells by folate-conjugated CdTe/CdS Quantum Dots Chiral Nano-Sensors.

Biochem Biophys Res Commun 2021 Jun 14;560:199-204. Epub 2021 May 14.

Department of Hepatobiliary Surgery, Tianjin Medical University Cancer Institute & Hospital, Tianjin, 300060, China; National Clinical Research Center for Cancer, Tianjin, 300060, China; Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China; Department of Hepatic Surgery, Fudan University Shanghai Cancer Center, Shanghai Medical College, Fudan University, Shanghai, 200032, PR China. Electronic address:

The specific identification and elimination of cancer cells has been a great challenge in the past few decades. In this study, the circular dichroism (CD) of cells was measured by a self-designed special system through the folate-conjugated chiral nano-sensor. A novel method was established to recognize cancer cells from normal cells according to the chirality of cells based on their CD signals. After a period of interaction between the nano-sensor and cells, the sharp weakening of CD signals was induced in cancer cells but normal cells remained unchanged. The biocompatibility of the nano-sensor was evaluated and the result showed that it exhibited significant cytotoxic activity against cancer cells while no obvious damage on normal cells. Notably, the research indicated that the nano-sensor may selectively cause apoptosis in cancer cells, and thus, have the potential to act as an antitumor agent.
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http://dx.doi.org/10.1016/j.bbrc.2021.04.068DOI Listing
June 2021

Role of Transient Receptor Potential Vanilloid 4 in Vascular Function.

Front Mol Biosci 2021 26;8:677661. Epub 2021 Apr 26.

Wuxi School of Medicine, Jiangnan University, Wuxi, China.

Transient receptor potential vanilloid 4 (TRPV4) channels are widely expressed in systemic tissues and can be activated by many stimuli. TRPV4, a Ca-permeable cation channel, plays an important role in the vasculature and is implicated in the regulation of cardiovascular homeostasis processes such as blood pressure, vascular remodeling, and pulmonary hypertension and edema. Within the vasculature, TRPV4 channels are expressed in smooth muscle cells, endothelial cells, and perivascular nerves. The activation of endothelial TRPV4 contributes to vasodilation involving nitric oxide, prostacyclin, and endothelial-derived hyperpolarizing factor pathways. TRPV4 activation also can directly cause vascular smooth muscle cell hyperpolarization and vasodilation. In addition, TRPV4 activation can evoke constriction in some specific vascular beds or under some pathological conditions. TRPV4 participates in the control of vascular permeability and vascular damage, particularly in the lung capillary endothelial barrier and lung injury. It also participates in vascular remodeling regulation mainly by controlling vasculogenesis and arteriogenesis. This review examines the role of TRPV4 in vascular function, particularly in vascular dilation and constriction, vascular permeability, vascular remodeling, and vascular damage, along with possible mechanisms, and discusses the possibility of targeting TRPV4 for therapy.
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http://dx.doi.org/10.3389/fmolb.2021.677661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107436PMC
April 2021

Exosomal circ_0030167 derived from BM-MSCs inhibits the invasion, migration, proliferation and stemness of pancreatic cancer cells by sponging miR-338-5p and targeting the Wif1/Wnt8/β-catenin axis.

Cancer Lett 2021 Aug 7;512:38-50. Epub 2021 May 7.

Research Center of Clinical Medical and Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu province, 226001, PR China. Electronic address:

Pancreatic cancer (PC) is one of the most lethal malignant tumors and has the lowest survival rate due to early metastasis and drug resistance. Exosomes derived from bone marrow mesenchymal stem cells (BM-MSCs) have emerged as crucial regulators of the progression of various tumors. These vesicles contain abundant circRNAs that have important biological functions. This study aimed to elucidate the role of exosomal circRNAs in PC progression. In this study, we successfully isolated BM-MSCs from human bone marrow based on their surface marker expression and osteogenic and adipogenic differentiation potential. We found that BM-MSC-derived exosomes significantly reduced the invasion, migration, and proliferation of PC cells, as well as tumor stemness. According to whole-transcriptome resequencing and clustering heat map analysis, we identified the key molecule circ_0030167 and miR-338-5p, its downstream target. We revealed that circ_0030167 mainly regulates miR-338-5p, enhances Wif1 expression, and inhibits the Wnt8/β-catenin pathway, thereby inhibiting the stemness of PC cells and tumor progression. Overall, BM-MSC exosomal circ_0030167 contributes to the progression and stemness of PC cells via the miR-338-5p/wif1/Wnt 8/β-catenin axis. Our study provides a new perspective for the treatment of PC.
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http://dx.doi.org/10.1016/j.canlet.2021.04.030DOI Listing
August 2021

Dipeptidyl peptidase IV is required for endometrial carcinoma cell proliferation and tumorigenesis via the IL-6/STAT3 pathway.

J Obstet Gynaecol Res 2021 May 9. Epub 2021 May 9.

Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.

Aim: To study the functions and signaling pathways controlled by dipeptidyl peptidase IV (DPPIV) in endometrial carcinoma (EC).

Methods: DPPIV expression in EC cells was detected by flow cytometry, reverse transcription-polymerase chain reaction analysis and Western blot. Interleukin-6 (IL-6) expression in the supernatant was measured by enzyme-linked immunosorbent assay. The protein levels of signal transducers and activators of transcription-3 (STAT3), phosphorylate STAT3, cellular Myc, and vascular endothelial growth factor in EC cells were measured by Western blot. Colony formation assays were used to assess the clonogenicity of EC cells. Ki67 immunostaining and cell counting were used to test the proliferative ability of EC cells. Nude mouse tumorigenicity assay was used to confirm DPPIV promotes the tumorigenicity of EC cells. A cell counting kit-8 assay was used to determine the half-maximal inhibitory concentration of sitagliptin.

Results: Overexpression of DPPIV in EC cells with low DPPIV expression promoted cell proliferation in vitro (p < 0.01) and enhanced tumorigenicity in vivo (p < 0.05). Conversely, knocking down DPPIV expression in EC cells with high DPPIV expression inhibited cell proliferation (p < 0.01) and in vivo tumorigenicity (p < 0.01). DPPIV promoted EC cell proliferation via activation of IL-6/STAT3 signaling pathway, and that IL-6 could trigger a positive feedback loop that increased DPPIV expression (p < 0.01). Furthermore, the DPPIV inhibitor reduced STAT3 expression (p < 0.01) and inhibited growth of EC cells (p < 0.001).

Conclusion: DPPIV enhances the properties that allow tumorigenesis in EC via IL-6 and STAT3 signaling.
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http://dx.doi.org/10.1111/jog.14788DOI Listing
May 2021

Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis.

J Surg Res 2021 May 3;265:223-232. Epub 2021 May 3.

Department of orthopedics, Chengdu Second People's Hospital; Sichuau, China. Electronic address:

Background: Long non-coding RNAs (lncRNAs) have been demonstrated to be involved in the progression of sepsis-induced acute kidney injury (AKI). In this study, we aimed to explore the functions of lncRNA cancer susceptibility candidate 2 (CASC2) in sepsis-induced AKI.

Methods: The sepsis cell models were established by exposing HK2 and HEK293 cells into lipopolysaccharide (LPS). Quantitative real-time polymerase chain reaction (qRT-PCR) assay was conducted to determine the expression of CASC2, miR-545-3p and peroxisome proliferator-activated receptor-α (PPARA) mRNA. Cell Counting Kit-8 (CCK-8) assay, flow cytometry analysis and wound healing assay were employed for cell viability, apoptosis and migration, respectively. Western blot assay was conducted for the protein levels of E-cadherin, α-SMA and PPARA. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured by specific kits. The relationship between miR-545-3p and CASC2 or PPARA was verified by dual-luciferase reporter assay.

Results: CASC2 level was decreased in sepsis patients' serums and LPS-treated HK2 and HEK293 cells. CASC2 overexpression facilitated cell viability and restrained cell apoptosis, migration, epithelial-mesenchymal transition (EMT) and oxidative stress in LPS-triggered HK2 and HEK293 cells. CASC2 was identified as a sponge for miR-545-3p to regulate PPARA expression. MiR-545-3p overexpression restored the impact of CASC2 on LPS-induced injury in HK2 and HEK293 cells. Moreover, miR-545-3p overexpression aggravated LPS-induced cell injury in HK2 and HEK293 cells by targeting PPARA.

Conclusion: CASC2 overexpression relieved the damage of HK2 and HEK293 cells mediated by LPS treatment through regulating miR-545-3p/PPARA axis.
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http://dx.doi.org/10.1016/j.jss.2021.03.047DOI Listing
May 2021

MEX3A suppresses proliferation and EMT via inhibiting Akt signaling pathway in cervical cancer.

Am J Cancer Res 2021 15;11(4):1446-1462. Epub 2021 Apr 15.

Center for Uterine Cancer Diagnosis & Therapy Research of Zhejiang Province, Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, People's Republic of China.

MEX3A, one member of the human MEX3 gene family, exerts different effects on a variety of human cancer cells. However, the biological functions and regulatory mechanism have not been explored in cervical cancer. In our study, we used multiple approaches to determine the functions and underlying molecular mechanism of MEX3A in cervical tumorigenesis, including CCK-8 assay, BrdU assay, FACS for cell cycle and apoptosis, wound healing assay, Transwell migration and invasion assays, immunohistochemistry (IHC) assay, Transfection, real-time RT-PCR and Western blotting analysis. IHC results showed that the expression levels of MEX3A were decreased in cervical cancer patients with advanced clinical stages and lymph node involvement. Moreover, upregulation of MEX3A attenuated cell proliferation, migration and invasion and induced cell cycle arrest at G0/G1 phase in human cervical cancer cells, whereas knockdown of MEX3A exhibited the opposite effects. Mechanistically, MEX3A exerted its tumor suppressive functions via inactivation of Akt signaling pathway and inhibiting epithelial to mesenchymal transition (EMT). Importantly, Akt activation by its activator SC79 reversed the biological functions of MEX3A overexpression. Furthermore, MEX3A inhibited tumor growth in xenograft models. Overall, our investigation suggested that MEX3A participated in antitumor activity in cervical cancer by inhibition of the Akt signaling pathway and EMT. Hence, targeting MEX3A might have a therapeutic potential to treat cervical cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085868PMC
April 2021

Reduction of microwave ablation needle related metallic artifacts using virtual monoenergetic images from dual-layer detector spectral CT in a rabbit model with VX2 tumor.

Sci Rep 2021 Apr 29;11(1):9295. Epub 2021 Apr 29.

Department of Radiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No.1 Shuaifuyuan Wangfujing Dongcheng District, Beijing, 100730, China.

The purpose of the study was to investigate the application of virtual monoenergetic images (VMIs) in reducing metal artifacts in rabbit VX2 liver cancer models treated with microwave ablation (MWA) therapy. A total of 31 VX2 liver cancer models that accepted CT-guided percutaneous microwave ablation were analyzed. Conventional images (CIs) with the most severe metallic artifacts and their corresponding energy levels from 40 to 200 keV with 10 keV increment of VMIs were reconstructed for further analysis. Objective image analysis was assessed by recording the attenuation (HU) and standard deviation of the most severe hyper/hypodense artifacts as well as artifact-impaired liver parenchyma tissue. Two radiologists visually evaluated the extent of artifact reduction, assessed data obtained by a diagnostic evaluation of liver tissues, and appraised the appearance of new artifacts according to the grade score. Statistical analysis was performed to compare the difference between CIs and each energy level of VMIs. For subjective assessment, reductions in hyperdense and hypodense artifacts were observed at 170-200 keV and 160-200 keV, respectively. The outcomes of the diagnostic evaluation of adjacent liver tissue were statistically higher at 140-200 keV for VMIs than for CIs. In terms of objective evaluation results, VMIs at 90-200 keV reduced the corrected attenuation of hyperdense and of artifact-impaired liver parenchyma compared with CIs (P < 0.001). When VMIs at 80-200 keV decreased the hypodense artifacts (P < 0.001). Therefore, we concluded that VMIs at 170-200 keV can obviously decrease the microwave ablation needle-related metal artifacts objectively and subjectively in rabbit VX2 liver cancer models.
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http://dx.doi.org/10.1038/s41598-021-88853-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085209PMC
April 2021

Flexible Supercapacitors Based on Graphene/Boron Nitride Nanosheets Electrodes and PVA/PEI Gel Electrolytes.

Materials (Basel) 2021 Apr 14;14(8). Epub 2021 Apr 14.

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-Nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 101400, China.

All-solid-state supercapacitors have gained increasing attention as wearable energy storage devices, partially due to their flexible, safe, and lightweight natures. However, their electrochemical performances are largely hampered by the low flexibility and durability of current polyvinyl alcohol (PVA) based electrolytes. Herein, a novel polyvinyl alcohol-polyethyleneimine (PVA-PEI) based, conductive and elastic hydrogel was devised as an all-in-one electrolyte platform for wearable supercapacitor (WSC). For proof-of-concept, we assembled all-solid-state supercapacitors based on boron nitride nanosheets (BNNS) intercalated graphene electrodes and PVA-PEI based gel electrolyte. Furthermore, by varying the electrolyte ions, we observed synergistic effects between the hydrogel and the electrode materials when KOH was used as electrolyte ions, as the Graphene/[email protected] WSCs exhibited a significantly improved areal capacitance of 0.35 F/cm and a smaller ESR of 6.02 ohm/cm. Moreover, due to the high flexibility and durability of the PVA-PEI hydrogel electrolyte, the developed WSCs behave excellent flexibility and cycling stability under different bending states and after 5000 cycles. Therefore, the conductive, yet elastic, PVA-PEI hydrogel represents an attractive electrolyte platform for WSC, and the Graphene/[email protected] WSCs shows broad potentials in powering wearable electronic devices.
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http://dx.doi.org/10.3390/ma14081955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069789PMC
April 2021

Hydrogel-coated needles prevent puncture site bleeding.

Acta Biomater 2021 Apr 22. Epub 2021 Apr 22.

The Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT, USA; Departments of Surgery and of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, CT, USA. Electronic address:

Introduction: Incomplete hemostasis after vascular cannulation can cause a hematoma or pseudoaneurysm. We hypothesized that a hydrogel-coated needle would effectively and rapidly stop bleeding after vascular cannulation.

Methods: A hydrogel composed of sodium alginate, hyaluronic acid, and calcium carbonate was coated onto the surface of suture needles. Needles were observed using scanning electronic microscopy (SEM) and immunofluorescence. Cannulation was performed in both mouse and rat models; the liver, kidney, jugular vein, inferior vena cava and aorta were punctured using uncoated and hydrogel-coated needles. Needles coated with a hydrogel with and without CD34 antibody were used to puncture the rat jugular vein and aorta. Tissues were examined by histology and immunofluorescence.

Results: The hydrogel was successfully coated onto the surface of 22G and 30G needles and confirmed by SEM. Hydrogel-coated needles rapidly stopped bleeding after cannulation of the liver, kidney, jugular vein, inferior vena cava and aorta. Hydrogel-coated needles that contained CD34 antibody attracted vascular progenitor cells near the puncture site; there were fewer M1-type macrophages and more M2-type macrophages.

Conclusion: Hydrogel-coated needles can effectively and rapidly stop puncture-site bleeding. The hydrogel that contains CD34 antibody attracted vascular progenitor cells, potentially promoting healing of the site after cannulation.

Statement Of Significance: Incomplete hemostasis after vascular cannulation can cause a hematoma or pseudoaneurysm and remains a significant clinical problem. We developed a hydrogel composed of sodium alginate, hyaluronic acid, and calcium carbonate; hydrogel-coated needles effectively and rapidly stopped bleeding after vascular cannulation. Interestingly, the hydrogel can also serve as a carrier for drugs that are delivered to the puncture site during the short time of cannulation that could additionally promote puncture site healing. Hydrogel-coated needles may be a new method for rapid hemostasis with application to patients especially at risk for bleeding.
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http://dx.doi.org/10.1016/j.actbio.2021.04.004DOI Listing
April 2021

Highly Active and Stable Palladium Catalysts on Novel Ceria-Alumina Supports for Efficient Oxidation of Carbon Monoxide and Hydrocarbons.

Environ Sci Technol 2021 Jun 19;55(11):7624-7633. Epub 2021 Apr 19.

Department of Civil, Environmental, and Construction Engineering, Catalysis Cluster for Renewable Energy and Chemical Transformations (REACT), NanoScience Technology Center (NSTC), University of Central Florida, Orlando, Florida 32816, United States.

Precious metal catalysts with superior low-temperature activity and excellent thermal stability are highly needed in environmental catalysis field. In this work, a novel two-step incipient wetness impregnation (T-IWI) method was developed for the fabrication of a unique and highly stable CeO/AlO support (CA-T). Pd anchored on CA-T exhibited a much higher low-temperature catalytic activity and superior thermal stability in carbon monoxide (CO) and hydrocarbon (HC) oxidations, compared to Pd anchored on conventional CeO/AlO (CA), which was prepared by a one-step IWI method. After aging treatment at 800 °C, the CO oxidation rate on Pd/CA-T (1.69 mmol/(g s)) at 120 °C was 4.1 and 84.5 times of those on Pd/CA (0.41 mmol/(g s)) and Pd/AlO (0.02 mmol/(g s)), respectively. It was revealed that the CA-T support with well-controlled small CeO particles ( 12 nm) possessed abundant defects for Pd anchoring, which created rich Pd-CeO interfaces with strengthened interaction between Pd and CeO where oxygen could be efficiently activated. This resulted in the significantly improved oxidation activity and thermal stability of Pd/CA-T catalysts. The T-IWI method developed herein can be applied as a universal approach to prepare highly stable metal oxide-alumina-based supports, which have broad application in environmental catalyst design, especially for automobile exhaust aftertreatment.
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http://dx.doi.org/10.1021/acs.est.1c00077DOI Listing
June 2021

MiR-573 suppresses cell proliferation, migration and invasion via regulation of E2F3 in pancreatic cancer.

J Cancer 2021 19;12(10):3033-3044. Epub 2021 Mar 19.

Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.

Pancreatic cancer is among the most lethal malignancies worldwide. In this study, we aimed to determine whether miR-573 could suppress pancreatic cancer cell proliferation, migration, and invasion by targeting E2F3. MiR-573 expression in pancreatic cancer tissues and cell lines was measured using real-time PCR. Target genes of miR-573 were screened using bioinformatics tools and confirmed using dual-luciferase reporter assay and real-time PCR. Pancreatic cancer cells were transfected using an miR-573 mimic or siRNA E2F3. Furthermore, cell proliferation, migration, and invasion were assessed using CCK-8, Edu staining, colony-forming assay, wound healing assay, and transwell assay . The effects of miR-573 were verified using tumor xenografts. Differential expression and prognostic analyses of miR-573 and E2F3 were visualized using the Kaplan‑Meier plotter and GEPIA. We found that the expression of miR-573 was significantly reduced in pancreatic cancer tissues and cell lines. Overexpression of miR-573 obviously suppressed the proliferation, migration, and invasion of pancreatic cancer cells. The Dual-luciferase assay showed that miR-573 could specifically target E2F3. Furthermore, E2F3 was up-regulated in pancreatic cancer tissues and cell lines and E2F3 down-regulation inhibited the proliferation, migration, and invasion of pancreatic cancer cells. The ectopic expression of miR-573 inhibited xenograft tumor growth . Results from the Kaplan-Meier analysis and GEPIA showed that patients with a high level of miR-573 had a significantly reduced risk of death while those with a high level of E2F3 displayed significant correlation with the tumor stage and suffered worse prognosis. MiR-573 could suppress the proliferation, migration, and invasion of pancreatic cancer cells by targeting E2F3, thereby establishing miR-573 as a novel regulator of E2F3 and indicating its critical role in tumorigenesis, especially in pancreatic cancer.
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http://dx.doi.org/10.7150/jca.51147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040892PMC
March 2021

In Situ Laser Fenestration for Delayed Left Subclavian Artery Revascularization Following Thoracic Endovascular Aortic Repair of Type B Aortic Dissection.

Vasc Endovascular Surg 2021 Apr 13:15385744211010386. Epub 2021 Apr 13.

Department of Vascular and Endovascular Surgery, 191599First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

In situ laser fenestration (ISLF) is currently used to reconstruct the aortic major branches during thoracic endovascular aortic repair (TEVAR). To our knowledge, there have been no reports on the application of ISLF for delayed revascularization of the LSA previously sealed in TEVAR. This report describes 5 patients who underwent ISLF for delayed LSA revascularization, with a technical success rate of 80%. No endoleakage occurred, and stents remained patent during more than 6-month follow-up. ISLF is an effective, safe and minimally invasive method for delayed revascularization of the LSA following TEVAR for type B aortic dissection (TBAD) when patients are selected appropriately.
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http://dx.doi.org/10.1177/15385744211010386DOI Listing
April 2021

[Genetic study of a child carrying a maternally derived unbalanced 46,Y,der(X)t(X;Y)(p22;q11) chromosomal translocation].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Apr;38(4):376-379

Lianyungang Maternal and Child Health Care Hospital, Lianyungang, Jiangsu 222000, China.

Objective: To explore the genetic basis for a child featuring short stature, saddle nose, cryptorchidism and mental retardation.

Methods: The child and his parents were subjected to G-banded karyotyping and chromosomal microarray analysis (CMA).

Results: The child was found to have a 46,Y,der(X)t(X;Y)(p22;q11)mat karyotype. CMA has revealed a 8.3 Mb deletion at Xp22.33p22.31 and a 43.3 Mb duplication at Yq11.221qter. His mother had a karyotype of 46,X,der(X)t(X;Y)(p22;q11). His father had a normal karyotype.

Conclusion: The child has carried an unbalanced translocation der(X)t(X;Y) (p22;q11) derived from his mother. His clinical phenotype has correlated with the size and position of X chromosome deletion. Compared with the females, abnormal phenotypes such as mental retardation and growth retardation of male carriers are more severe.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200321-00188DOI Listing
April 2021

Free-triplet generation with improved efficiency in tetracene oligomers through spatially separated triplet pair states.

Nat Chem 2021 Jun 8;13(6):559-567. Epub 2021 Apr 8.

National Laboratory of Solid State Microstructures, School of Physics and Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, China.

Singlet fission (SF) can potentially boost the efficiency of solar energy conversion by converting a singlet exciton (S) into two free triplets (T + T) through an intermediate state of a correlated triplet pair (TT). Although efficient TT generation has been recently realized in many intramolecular SF materials, their potential applications have been hindered by the poor efficiency of TT dissociation. Here we demonstrate that this can be overcome by employing a spatially separated (T…T) state with weak intertriplet coupling in tetracene oligomers with three or more chromophores. By using transient magneto-optical spectroscopic methods, we show that free-triplet generation can be markedly enhanced through the SF pathway that involves the spatially separated (T…T) state rather than the pathway mediated by the spatially adjacent TT state, leading to a marked improvement in free-triplet generation with an efficiency increase from 21% for the dimer to 85% (124%) for the trimer (tetramer).
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http://dx.doi.org/10.1038/s41557-021-00665-7DOI Listing
June 2021

Conversion Therapy of Intrahepatic Cholangiocarcinoma Is Associated with Improved Prognosis and Verified by a Case of Patient-Derived Organoid.

Cancers (Basel) 2021 Mar 9;13(5). Epub 2021 Mar 9.

Department of Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.

This study was performed to determine the efficacy of conversion therapy in intrahepatic cholangiocarcinoma (IHCC) and explore the feasibility of cancer organoid to direct the conversion therapy of IHCC. Patient data were retrospectively reviewed in this study and cancer organoids were established using tissues obtained from two patients. A total of 42 patients with IHCC received conversion therapy, 9 of whom were downstaged successfully, and another 157 patients were initially resectable. Kaplan-Meier curves showed that the successfully downstaged patients had a significantly improved overall survival compared to those in whom downstaging was unsuccessful ( = 0.017), and had a similar overall survival to that of initially resectable patients ( = 0.965). The IHCC organoid was successfully established from one of two obtained tissues. Routine hematoxylin and eosin staining and immunohistological staining found the organoid retained the histopathological characteristics of the original tissues. Whole exome sequencing results indicated the IHCC organoid retained appropriately 87% of the variants in the original tissue. Gemcitabine and paclitaxel exhibited the strongest inhibitory effects on the cancer organoid as determined using drug screening tests, consistent with the levels of efficacy observed in the patient from whom it was derived. This study indicates that conversion therapy could improve the survival of patients with IHCC despite its low success rate, and it may be directed by cancer organoids though this is merely a proof of feasibility.
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http://dx.doi.org/10.3390/cancers13051179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967139PMC
March 2021

Efficacy of electrochemical membrane bioreactor for virus removal from wastewater: Performance and mechanisms.

Bioresour Technol 2021 Jun 10;330:124946. Epub 2021 Mar 10.

State Key Laboratory of Pollution Control and Resource Reuse, Shanghai Institute of Pollution Control and Ecological Security, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China; International Joint Research Center for Sustainable Urban Water System, Shanghai 200092, China. Electronic address:

Wastewater treatment facilities play pivotal roles in preventing the transmission of water-borne viruses and protecting human health. In this study, a new electrochemical membrane bioreactor (EMBR) was proposed to achieve a long-lasting and efficient removal of virus from wastewater. Results showed that applying a low electric field (2.0 V) in EMBR system could achieve ~100% removal efficiency at both batch tests and continuous flow experiments. In contrast, the control MBR, without the exertion of electric field, exhibited a very low removal efficiency (19.8% on average). Moreover, the fouling in EMBR was significantly mitigated, which enabled its operation duration almost 3 times longer than that of the control. Further explorations suggested that the reactive oxidants generated on electrodes in the EMBR system were mainly responsible for MS2 removal. This study demonstrated the potential of utilizing the EMBR process to achieve an enhanced virus disinfection efficiency during the wastewater treatment process.
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http://dx.doi.org/10.1016/j.biortech.2021.124946DOI Listing
June 2021

Application of prosthesis eversion method for ascending aorta replacement guarantees better clinical outcomes of type A acute aortic dissection surgery.

J Thorac Dis 2021 Feb;13(2):533-540

Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Background: The advantages of prosthesis eversion method in patients diagnosed with Stanford type A acute aortic dissection (AAD) undergoing ascending aorta replacement (AAR) is unknown. This research is designed to explore it.

Methods: We retrospectively analyzed the data of a total of 283 patients diagnosed with type A aortic dissection that underwent surgery in Renmin Hospital of Wuhan University from March, 2006 to April, 2020. Eighty-eight patients underwent surgical repair with traditional continuous suture technique, and 195 patients received prosthesis eversion. Baseline data, intra-operative data and early-stage clinical results were collected and statistically analyzed.

Results: Baseline data were similar except for age, incidence of hyperlipidemia and taking ACEI/ARB drugs (P<0.05). Cardiopulmonary bypass time, cross-clamp time, circulation arrest time, hemostasis time and total operation time in the traditional method group were far longer than in the prothesis eversion group (P<0.01). The operative mortality was similar (P>0.01). Post-operatively, there was no statistically significant difference in the mean ventilation time, mortality, incidence of re-exploration, tracheostomy, paraplegia, long-term coma and stroke between the two groups (P>0.05). Patients in the traditional method group had a longer duration stay in ICU and hospital than patients in the prosthesis eversion group (P<0.05). Patients in the traditional method group received more red blood cells (RBC) (P<0.01), plasma (P<0.05), fibrinogen (P<0.01) and albumin (P<0.05) transfusions, and CoSeal™ surgical sealant (P<0.05) than patients in the prosthesis eversion group.

Conclusions: Our experience and statistical analysis showed prosthesis eversion method to have some advantage in reducing blood loss and improving clinical results compared with repair with continuous suture. This technique is both simple to learn and perform.
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http://dx.doi.org/10.21037/jtd-20-2578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947529PMC
February 2021

Increased Serum E-Selectin Levels Were Associated with Cognitive Decline in Patients with Stroke.

Int J Gen Med 2021 2;14:733-739. Epub 2021 Mar 2.

Department of Neurology, The First Hospital of Hebei Medical University, Shijiazhuang, 050030, Hebei, People's Republic of China.

Background: Previous studies have reported that patients with stroke have a high incidence of cognitive decline. The aim was to elucidate the association between serum E-selectin levels and cognitive function in stroke patients.

Materials And Methods: Serum levels of E-selectin were measured in 322 patients with stroke at baseline. Cox proportional hazard analysis was used to evaluate the predictive value of serum E-selectin for predicting cognitive decline (end point) in patients with stroke.

Results: Multivariate linear regression analysis revealed that serum E-selectin levels were independently associated with MOCA score after adjusting for age, gender, BMI, current smoker, current drinker, admission systolic and diastolic BP, CVD history and laboratory measurements in patients with stroke at baseline (Sβ= -0.156; 95% CI, - 0.170- - 0.074; 0.001). The multivariate Cox proportional hazard analysis revealed that serum E-selectin (HR=2.481, 95% CI 1.533-4.327, -trend <0.001) was an independent prognostic factor for cognitive decline in these patients with stroke during the follow-up period.

Conclusion: Our results showed that increased serum E-selectin levels were significantly and independently associated with cognitive decline and had independent predictive value for cognitive decline in patients with stroke. Serum E-selectin might enable early recognition of cognitive decline among stroke patients.
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http://dx.doi.org/10.2147/IJGM.S292171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936714PMC
March 2021

[email protected] and lateral heterostructures composed of SnS and NbS.

Nanoscale 2021 Mar;13(10):5489-5496

Frontiers Science Center for Flexible Electronics, Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials & Engineering, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an 710072, China. and Institute of Advanced Materials (IAM), Nanjing Tech University (Nanjing Tech), 30 South Puzhu Road, Nanjing 211816, China.

The spatial arrangement of heterostructures based on two-dimensional layered materials is important in controlling their electronic and optoelectronic properties. In this contribution, by controlling the reaction kinetics and thus the nucleation and growth sequence of p-type SnS and metallic NbS2, controllable preparation of both [email protected] [email protected] and SnS/NbS2 lateral heterostructures was realized. The [email protected] [email protected] heterostructures were further applied in photodetectors, and interestingly, a negative photoresponse was observed due to the Seebeck effect exerted on the NbS2 shell. Compared with the pure metallic NbS2, the [email protected] [email protected] heterostructures showed a 15 times increased signal-to-noise ratio and much improved photocurrent stability, largely due to the charge and heat transfer between the SnS core and NbS2 shell.
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http://dx.doi.org/10.1039/d0nr08415hDOI Listing
March 2021

An anaerobic dynamic membrane bioreactor for enhancing sludge digestion: Impact of solids retention time on digestion efficacy.

Bioresour Technol 2021 Jun 20;329:124864. Epub 2021 Feb 20.

State Key Laboratory of Pollution Control and Resource Reuse, Shanghai Institute of Pollution Control and Ecological Security, School and Environment of Science and Engineering, Tongji University, Shanghai 200092, China. Electronic address:

An anaerobic dynamic membrane bioreactor (AnDMBR), which enabled the decoupling of hydraulic retention time (HRT) and solids retention time (SRT), was used for enhancing sludge digestion, with the associated mechanisms elucidated. With the increase of SRT, the biogas production and sludge reduction rate were both enhanced. The specific biogas production and volatile solids (VS) reduction rate were improved to 0.79 L/g VS and 55.9% under SRT 50 d, respectively. Microbial community analysis revealed that Chloroflexi, which is capable of degrading metabolites and dead cells, was enriched at longer SRT. Further analysis showed that both acetoclastic and hydrogenotrophic methanogenesis contributed to the enhanced biogas production under higher SRT compared to the dominance of acetoclastic methanogenesis under lower SRT. The enhanced utilization of organic matter and acetate at longer SRT further confirmed the mechanisms. The results highlighted the potential of AnDMBR for high-efficient sludge digestion.
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http://dx.doi.org/10.1016/j.biortech.2021.124864DOI Listing
June 2021

Removal of Matrix Interferences by Nano-MgO and Co-Adsorbents for Accurate Multi-Pesticide Residue Analysis in the Chinese Medicinal Herb, .

J Anal Methods Chem 2021 4;2021:6626257. Epub 2021 Feb 4.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Quality and Standard of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

A simple, accurate, and high-throughput analytical method was developed to detect 123 pesticide residues in Chinese medicinal herb (PRA) by introducing nano-MgO as a highly efficient purification material based on quick, easy, cheap, effective, rugged, and safe (QuEChERS) design concept. Various PRA samples were extracted using 8 mL 0.5% acetic acid-acetonitrile solution and purified by a dispersive solid-phase extraction method with 30 mg nano-MgO, 40 mg primary secondary amine (PSA), and 40 mg octadecylsilane (C18) as the cleanup adsorbents, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). 70.7% of pesticides showed a weak matrix effect after the purification process, indicating that this method can give the precise quantitative analysis of trace pesticides residue. The method was systematically validated under optimal conditions in five different kinds of PRA samples; good linearity was observed in the concentration range of 0.5-250 g/L or 1-250 g/L. Pesticide recovery in each sample spiked at concentrations of 20, 50, and 200 g/kg ranged from 98.0% to 111% and the mean relative standard deviation ranged from 2.72% to 5.70%. Furthermore, the method comparison with the traditional QuEChERS method suggested the feasibility, advantages, and potential application prospect of the present method for the multi-pesticide residue analysis in various PRA samples.
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http://dx.doi.org/10.1155/2021/6626257DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880715PMC
February 2021

Gut microbiota alterations associated with antibody-mediated rejection after kidney transplantation.

Appl Microbiol Biotechnol 2021 Mar 24;105(6):2473-2484. Epub 2021 Feb 24.

Department of Urology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, 450003, China.

Antibody-mediated rejection (AMR) has become the major challenge for kidney transplantation, and the efficacy of existing therapies was limited to prevent AMR. Increasing evidences have demonstrated the link between gut microbiota alterations and allograft outcome. However, there has been no comprehensive analysis to profile the gut microbiota associated with AMR after kidney transplantation. We performed this study to characterize the gut microbiota possibly associated with AMR. Fecal specimens were collected from 24 kidney transplantation recipients with AMR and 29 controls. DNA extracted from the specimens was processed for 16S rRNA gene sequencing using Illumina MiSeq. Gut microbial community of recipients with AMR was significantly different from that of controls based on unweighted (P = 0.001) and weighted (P = 0.02) UniFrac distances, and the bacterial richness (observed species: P = 0.0448; Chao1 index: P = 0.0450; ACE index: P = 0.0331) significantly decreased in the AMR group. LEfSe showed that 1 phylum, 5 classes, 7 families, and 10 genera were increased, whereas 1 class, 2 order, 3 families, and 4 genera were decreased in the AMR group. Specific taxa such as Clostridiales could be potentially used as biomarkers to distinguish the recipients with AMR from the controls (AUC = 0.77). PICRUSt analysis illustrated that 16 functional pathways were with significantly different abundances in the AMR and control groups. Our findings provide a foundation for further investigation on the role of gut microbiota in AMR after kidney transplantation, and potentially support novel diagnostic biomarkers and therapeutic options for AMR. KEY POINTS: • Gut microbial community of kidney recipients with AMR was different from that of controls. • Clostridiales is a potential marker to distinguish recipients with AMR from controls.
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http://dx.doi.org/10.1007/s00253-020-11069-xDOI Listing
March 2021