Publications by authors named "Zhiwei Wang"

1,028 Publications

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Research progress in external field intensification of forward osmosis process for water treatment: A critical review.

Water Res 2022 Aug 2;222:118943. Epub 2022 Aug 2.

College of Environmental Science and Engineering, Key Laboratory of Science & Technology of Eco-Textile, Ministry of Education, Donghua University, Shanghai 201620, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, China. Electronic address:

Forward osmosis (FO) is an emerging permeation-driven membrane technology that manifests advantages of low energy consumption, low operating pressure, and uncomplicated engineering compared to conventional membrane processes. The key issues that need to be addressed in FO are membrane fouling, concentration polarization (CP) and reverse solute diffusion (RSD). They can lead to problems about loss of draw solutes and reduced membrane lifetime, which not only affect the water treatment effectiveness of FO membranes, but also increase the economic cost. Current research has focused on FO membrane preparation and modification strategies, as well as on the selection of draw solutions. Unfortunately, these intrinsic solutions had limited success in unraveling these phenomena. In this paper, we provide a brief review of the current state of research on existing external field-assisted FO systems (including electric-, pressure-, magnetic-, ultrasonic-, light- and flow-assisted FO system), analyze their mitigation mechanisms for the above key problems, and explore potential research directions to aid in the further development of FO systems. This review aims to reveal the feasibility of the development of external field-assisted FO technology to achieve a more economical and efficient FO treatment process.
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http://dx.doi.org/10.1016/j.watres.2022.118943DOI Listing
August 2022

High zeolite loading mixed matrix membrane for effective removal of ammonia from surface water.

Water Res 2022 Aug 11;221:118849. Epub 2022 Jul 11.

State Key Laboratory of Pollution Control and Resource Reuse, Shanghai Institute of Pollution Control and Ecological Security, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Tongji Advanced Membrane Technology Center, Shanghai 200092, China. Electronic address:

While zeolite-based mixed matrix membrane (MMM) has been proven effective to remove the ammonia in the wastewater by adsorption, its adsorption capacity is limited by the low zeolite loading due to the need of a high concentration of polymer matrix to maintain the mechanical strength. To break the bottleneck, in this study we proposed a facile solvent evaporation method instead of conventional phase inversion method to prepare the zeolite-based MMMs. With this new preparation method, the loading of zeolite could reach up to ∼90wt.% while the MMM still maintained a good mechanical property. The zeolite-based MMM could treat 910 L·m of feedwater before reaching the ammonia breakthrough point (0.5 mg-N·L) when treating the synthetic wastewater water. In addition, it showed a high rejection of turbidity and natural organic material (NOM) (∼90%), mainly due to its high negative surface charge density. When applied to treat real surface water, the membrane demonstrated a high normalized treatable capacity (∼900 L·m) with a high rejection to NOM (87.4%). Moreover, the MMM even showed a higher fouling resistance than the PVDF microfiltration membrane. Regeneration and cleaning with NaClO could efficiently recover the adsorption capacity and water flux of the MMM. After four cycles of operation, the MMM still maintained a high treatable capacity (850 L·m) with a high NOM rejection. This study provides a new strategy for the preparation of high-loading zeolite-based MMM for the effective removal of ammonia from surface water.
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http://dx.doi.org/10.1016/j.watres.2022.118849DOI Listing
August 2022

Realization of Oriented and Nanoporous Bismuth Chalcogenide Layers via Topochemical Heteroepitaxy for Flexible Gas Sensors.

Research (Wash D C) 2022 23;2022:9767651. Epub 2022 Jun 23.

Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, China.

Most van der Waals two-dimensional (2D) materials without surface dangling bonds show limited surface activities except for their edge sites. Ultrathin BiSe, a topological insulator that behaves metal-like under ambient conditions, has been overlooked on its surface activities. Herein, through a topochemical conversion process, ultrathin nanoporous BiSe layers were epitaxially deposited on BiOCl nanosheets with strong electronic coupling, leading to hybrid electronic states with further bandgap narrowing. Such oriented nanoporous BiSe layers possessed largely exposed active edge sites, along with improved surface roughness and film forming ability even on inkjet-printed flexible electrodes. Superior room-temperature NO sensing performance was achieved compared to other 2D materials under bent conditions. Our work demonstrates that creating nanoscale features in 2D materials through topochemical heteroepitaxy is promising to achieve both favorable electronic properties and surface activity toward practical applications.
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http://dx.doi.org/10.34133/2022/9767651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275095PMC
June 2022

Single-incision compared with conventional laparoscopy for appendectomy in acute appendicitis: a systematic review and meta-analysis.

Int J Colorectal Dis 2022 Aug 8. Epub 2022 Aug 8.

Department of Colorectal and Anal Surgery, The Affiliated Qingdao Central Hospital of Qingdao University, The Second Affiliated Hospital of Medical College of Qingdao University, 266042, Qingdao, Shandong, China.

Objective: A meta-analysis of the relevant literature evaluated the feasibility, safety, and potential benefits of single-incision laparoscopic appendectomy (SILA) relative to those of conventional laparoscopic appendectomy (CLA).

Methods: The major biomedical databases, including ClinicalTrials.gov, were searched up to January 2022 for relevant randomized controlled trials (RCTs). SILA and CLA were compared regarding patient body mass index, operative time, and perioperative complications. The Cochrane Handbook and RevMan 5.3 were used to judge trial quality and perform the meta-analysis, respectively.

Results: The 17 included RCTs comprised 2068 patients, of whom 1039 and 1029 patients underwent SILA and CLA, respectively. The operative time for SILA was longer than that for CLA (MD = 8.35 min, 95% CI = 6.58 to 10.11, P < 0.00001), but the cosmetic results from SILA were superior (SMD = 0.81, 95% CI = 0.58 to 1.03, P < 0.00001). However, the incidence rates were similar in terms of patient body mass index; postoperative pain scores; and rates of abdominal abscess, conversion to open surgery, ileus, surgical site infection, and overall perioperative complications between the two groups.

Conclusion: SILA is a safe technique for acute appendicitis, and its cosmetic outcomes are superior to those of CLA.
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http://dx.doi.org/10.1007/s00384-022-04231-6DOI Listing
August 2022

Physiological and Biochemical Responses of Pepper ( L.) Seedlings to Nickel Toxicity.

Front Plant Sci 2022 18;13:950392. Epub 2022 Jul 18.

Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, China.

Globally, heavy metal pollution of soil has remained a problem for food security and human health, having a significant impact on crop productivity. In agricultural environments, nickel (Ni) is becoming a hazardous element. The present study was performed to characterize the toxicity symptoms of Ni in pepper seedlings exposed to different concentrations of Ni. Four-week-old pepper seedlings were grown under hydroponic conditions using seven Ni concentrations (0, 10, 20, 30, 50, 75, and 100 mg L NiCl. 6HO). The Ni toxicity showed symptoms, such as chlorosis of young leaves. Excess Ni reduced growth and biomass production, root morphology, gas exchange elements, pigment molecules, and photosystem function. The growth tolerance index (GTI) was reduced by 88-, 75-, 60-, 45-, 30-, and 19% in plants against 10, 20, 30, 50, 75, and 100 mg L Ni, respectively. Higher Ni concentrations enhanced antioxidant enzyme activity, ROS accumulation, membrane integrity [malondialdehyde (MDA) and electrolyte leakage (EL)], and metabolites (proline, soluble sugars, total phenols, and flavonoids) in pepper leaves. Furthermore, increased Ni supply enhanced the Ni content in pepper's leaves and roots, but declined nitrogen (N), potassium (K), and phosphorus (P) levels dramatically. The translocation of Ni from root to shoot increased from 0.339 to 0.715 after being treated with 10-100 mg L Ni. The uptake of Ni in roots was reported to be higher than that in shoots. Generally, all Ni levels had a detrimental impact on enzyme activity and led to cell death in pepper seedlings. However, the present investigation revealed that Ni ≥ 30 mg L lead to a deleterious impact on pepper seedlings. In the future, research is needed to further explore the mechanism and gene expression involved in cell death caused by Ni toxicity in pepper plants.
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http://dx.doi.org/10.3389/fpls.2022.950392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340659PMC
July 2022

Moderate External Rotation of Tibial Component Generates More Natural Kinematics Than Internal Rotation After Total Knee Arthroplasty.

Front Bioeng Biotechnol 2022 13;10:910311. Epub 2022 Jul 13.

School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.

This study aimed to investigate the influence of tibial malrotation on knee kinematics after total knee arthroplasty (TKA). A symmetric fixed-bearing posterior-stabilized prosthesis was implanted in the validated knee model with different rotational alignments of the tibial component (neutral, 3° external rotation, 5° external rotation, 3° internal rotation, and 5° internal rotation). Computational kinematic simulations were used to evaluate the postoperative kinematics of the knee joint including anteroposterior translation femoral condyles and axial rotation of tibial component during 0°-135° knee flexion. The results revealed that the neutral position of the tibial component was not the closest kinematics to the intact knee, the model with 5° external rotation of the tibial component showed the closest lateral condyle translation and tibial axial rotation, and moderate external rotation could improve the kinematics after TKA.
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http://dx.doi.org/10.3389/fbioe.2022.910311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327137PMC
July 2022

Metagenomic Next-Generation Sequencing Reveals the Profile of Viral Infections in Kidney Transplant Recipients During the COVID-19 Pandemic.

Front Public Health 2022 11;10:888064. Epub 2022 Jul 11.

Department of Urology, Henan Provincial Clinical Research Center for Kidney Disease, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Henan University People's Hospital, Zhengzhou, China.

Background: To study the clinical application of metagenomic next-generation sequencing (mNGS) in the detection of viral infections in kidney transplant recipients (KTRs) during the COVID-19 pandemic.

Methods: Using mNGS technology, 50 human fluid samples of KTRs were detected, including 20 bronchoalveolar lavage fluid (BALF) samples, 21 urine samples and 9 blood samples. The detected nucleic acid sequences were compared and analyzed with the existing viral nucleic acid sequences in the database, and the virus infection spectrum of KTRs was drawn.

Results: The viral nucleic acids of 15 types of viruses were detected in 96.00% (48/50) of the samples, of which 11 types of viruses were in BALF (95.00%, 19/20), and the dominant viruses were (TTV) (65.00%; 13/20), (CMV) (45.00%; 9/20) and (25.00%; 5/20). 12 viruses (95.24%, 20/21) were detected in the urine, and the dominant viruses were TTV (52.38%; 11/21), (52.38%; 11/21), (42.86%; 9/21), CMV (33.33%; 7/21) and (28.57%; 6/21). 7 viruses were detected in the blood (100.00%, 9/9), and the dominant virus was TTV (100.00%; 9/9). Four rare viruses were detected in BALF and urine, including , and . Further analysis showed that TTV infection with high reads indicated a higher risk of acute rejection ( < 0.05).

Conclusions: mNGS detection reveals the rich virus spectrum of infected KTRs, and improves the detection rate of rare viruses. TTV may be a new biomarker for predicting rejection.
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http://dx.doi.org/10.3389/fpubh.2022.888064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309489PMC
July 2022

Surgeon Dominated Design Can Improve the Accuracy of Patient-Specific Instruments in Kinematically Aligned TKA.

J Pers Med 2022 Jul 22;12(8). Epub 2022 Jul 22.

The Center of Diagnosis and Treatment for Joint Disease, China Rehabilitation Research Center, Beijing 100068, China.

Precise bone resection is mandatory for kinematically aligned total knee arthroplasty (KA-TKA). This study is to investigate whether surgeon-dominated design can alter the accuracy of patient-specific instrumentation (PSI) in KA-TKA compared with the engineer design. A total of 24 patients (24 knees) who underwent KA-TKA in our institution were assigned to an engineer design group (10 knees) and surgeon design group (14 knees) chronologically. A novel portable medical-engineer interactive application can greatly enhance the surgeon's participation in PSI design. The bone resection discrepancies were used to evaluate the accuracy of PSI in bone resection. The overall discrepancy of bone resection was reduced by surgeon-designed PSI compared to engineer-designed PSI by 0.33 mm. Surgeon-designed PSI seems to reduce the outliers in terms of relative discrepancies in bone resection as well, but it does not reach statistical significance. Moreover, surgeon-designed PSI could significantly improve the accuracy of PSI in the restoration of the joint line in terms of medial proximal tibial angle and mechanical lateral distal femoral angle. This study indicates that the dominance of surgeons in both PSI design and subsequent surgical operation should be emphasized in efforts to improve the accuracy of PSI.
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http://dx.doi.org/10.3390/jpm12081192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332028PMC
July 2022

Iron deficiency promotes aortic media degeneration by activating endoplasmic reticulum stress-mediated IRE1 signaling pathway.

Pharmacol Res 2022 Jul 23;183:106366. Epub 2022 Jul 23.

Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, 238# Jiefang Road, Wuhan 430000, Hubei Province, China; Cardiovascular Surgery Laboratory, Renmin Hospital of Wuhan University, 9# Zhangzhidong Road, Wuhan 430000, Hubei Province, China; Central Laboratory, Renmin Hospital of Wuhan University, 9# Zhangzhidong Road, Wuhan 430000, Hubei Province, China.

Background: Aortic dissection (AD) is a macrovascular disease which is pathologically characterized by aortic media degeneration (AMD). Our team's previous research found that iron deficiency (ID) promoted the formation of AMD through presentative research. In this study, we aimed to investigate the underlying mechanism of ID promoting AMD formation.

Methods: The human aortic tissues were harvested from AD patients and organ donors. ApoE-/- mice were simultaneously given AngII infusion and low-iron feed to investigate the relationship between ID and AD. The IRE1-XBP1-CHOP signal axis of endoplasmic reticulum (ER) stress was selectively inhibited with 4μ8C. Iron contents were detected by Perls staining. The expression of iron metabolism and ER stress-relative proteins were analyzed by IF and western blotting. Apoptosis rates of aortic tissue and ASMCs were detected by TUNEL staining and flow cytometry, and ROS content was also measured by the flow cytometry.

Results: ID was accompanied by ER stress in patients with AD. Among the three signaling pathways of ER stress in ID-induced AMD, proteins of IRE1, PERK and ATF6 signaling pathways were up-regulated by 2.65 times, 1.14 times and 1.24 times, respectively. ID was positively related to ER stress, mitochondrial oxidative stress and aortic media apoptosis in vivo and in vitro assays, while 4μ8C reversed the severity of ER stress and AMD.

Conclusions: ID could activate ER stress by eliciting mitochondrial oxidative stress to activate the IRE1-XBP1-CHOP signaling pathway in the ER, which accelerated the apoptosis of ASMCs in aortic media, thus promoting the formation of AMD.
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http://dx.doi.org/10.1016/j.phrs.2022.106366DOI Listing
July 2022

Room-temperature epitaxial welding of 3D and 2D perovskites.

Nat Mater 2022 Jul 25. Epub 2022 Jul 25.

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), Nanjing, China.

Formation of epitaxial heterostructures via post-growth self-assembly is important in the design and preparation of functional hybrid systems combining unique properties of the constituents. This is particularly attractive for the construction of metal halide perovskite heterostructures, since their conventional solution synthesis usually leads to non-uniformity in composition, crystal phase and dimensionality. Herein, we demonstrate that a series of two-dimensional and three-dimensional perovskites of different composition and crystal phase can form epitaxial heterostructures through a ligand-assisted welding process at room temperature. Using the CsPbBr/PEAPbBr heterostructure as a demonstration, in addition to the effective charge and energy transfer across the epitaxial interface, localized lattice strain was observed at the interface, which was extended to the top layer of the two-dimensional perovskite, leading to multiple new sub-bandgap emissions at low temperature. Given the versatility of our strategy, unlimited hybrid systems are anticipated, yielding composition-, interface- and/or orientation-dependent properties.
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http://dx.doi.org/10.1038/s41563-022-01311-4DOI Listing
July 2022

Evaluation of Chiral Fungicide Penflufen in Legume Vegetables: Enantioseparation and Its Mechanism, Enantioselective Behaviors, and Risk Assessment.

J Agric Food Chem 2022 Aug 25;70(30):9319-9326. Epub 2022 Jul 25.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/ Key Laboratory of Detection for Pesticide Residues and Control of Zhejiang, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P. R. China.

Illustrating the enantioselective behaviors of the novel chiral fungicide penflufen was extremely important for ecological safety and human health. For penflufen enantiomers, an excellent separation method including a short analysis time (4 min), a high sensitivity (2 ng/g), and lesser consumption of an organic solvent was first established through supercritical fluid chromatography-tandem mass spectrometry. The enantioseparation mechanism was explained by computational chemistry, and the stronger binding ability of -(+)-penflufen with cellulose tris-(3-chloro-4-methylphenylcarbamate) (the chiral stationary phase OZ-3 column) contributed to the posterior elution. In legume vegetables, penflufen dissipation was the fastest in Linn plants (half-life, 1 day) and the slowest in plants (half-lives, 11.3-12.9 days). After 30, 50, and 40 days, the -penflufen residues were lower than the maximum residue level value in the Electronic Code of Federal Regulations (10 ng/g) in , Linn, and , respectively. Abundant -(+)-penflufen was found in these plants with stereoisomeric excess (se) changes being >10% in the initial stage, so the risk assessment might be driven by -(+)-penflufen. However, the se changes were <10% in plants, and the risk assessment might be calculated based on -penflufen. Moreover, penflufen enantiomers could be transferred from legume vegetables to soils, and the concentrations increased with time. The high persistence and medium mobility of penflufen in soils might lead to potential groundwater contamination, which was noteworthy. These results could contribute to a more accurate risk assessment of penflufen in legume vegetables.
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http://dx.doi.org/10.1021/acs.jafc.2c02238DOI Listing
August 2022

Boosting catalytic stability for VOCs removal by constructing PtCu alloy structure with superior oxygen activation behavior.

J Hazard Mater 2022 Jul 18;439:129612. Epub 2022 Jul 18.

Beijing Key Laboratory for Green Catalysis and Separation, Key Laboratory of Beijing on Regional Air Pollution Control, Key Laboratory of Advanced Functional Materials, Education Ministry of China, Department of Environmental Chemical Engineering, Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, China. Electronic address:

The elimination of volatile organic compounds (VOCs) emitted from the process of industry production is of great significance to improve the atmospheric environment. Herein the catalytic oxidation of the toluene and iso-hexane mixture, as the typical components from furniture paint industry, and the enhancement in the catalytic stability for toluene oxidation were investigated in detail. The formation rate of active oxygen species was very important for the development of the catalyst with high catalytic stability. Compared with the Pt/M catalyst, the Pt-Cu/M catalyst owned stronger ability of VOCs adsorption and gaseous oxygen activation by introducing additional sites for activating O. The Langmuir-Hinshelwood (adsorbed oxygen) and Mars-van Krevelen (lattice oxygen) mechanism existed in toluene oxidation over the present Pt/M and Pt-Cu/M catalysts, respectively. The change in the involved active oxygen species during toluene oxidation was resulted from the Pt-Cu alloy structure. In addition to the adsorption of O, a part of active lattice oxygen species can also be replenished by the migration of bulk lattice oxygen over Pt-Cu/M. With a rise in the reaction temperature, weakly adsorbed iso-hexane could be timely reacted with the more active lattice oxygen species to keep the catalytic stability over the Pt/M and Pt-Cu/M catalysts. Generally, we not only prepared a promising material for the catalytic removal of VOCs from the furniture paint industry, but also provided a new strategy for the generation of active oxygen species, making the catalyst exhibit high catalytic oxidation stability.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129612DOI Listing
July 2022

The Development of Microscopic Imaging Technology and its Application in Micro- and Nanotechnology.

Front Chem 2022 5;10:931169. Epub 2022 Jul 5.

Laboratory of Optical Detection and Imaging, School of Science, Qingdao University of Technology, Qingdao, China.

As a typical microscopic imaging technology, the emergence of the microscope has accelerated the pace of human exploration of the micro world. With the development of science and technology, microscopes have developed from the optical microscopes at the time of their invention to electron microscopes and even atomic force microscopes. The resolution has steadily improved, allowing humans to expand the field of research from the initial animal and plant tissues to microorganisms such as bacteria, and even down to the nanolevel. The microscope is now widely used in life science, material science, geological research, and other fields. It can be said that the development of microscopes also promotes the development of micro- and nanotechnology. It is foreseeable that microscopes will play a significant part in the exploration of the microworld for a long time to come. The development of microscope technology is the focus of this study, which summarized the properties of numerous microscopes and discussed their applications in micro and nanotechnology. At the same time, the application of microscopic imaging technology in micro- and nanofields was investigated based on the properties of various microscopes.
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http://dx.doi.org/10.3389/fchem.2022.931169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294601PMC
July 2022

The Combination of Rhodosin and MMF Prolongs Cardiac Allograft Survival by Inhibiting DC Maturation by Promoting Mitochondrial Fusion.

Oxid Med Cell Longev 2022 9;2022:7260305. Epub 2022 Jul 9.

Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China.

Despite being the gold-standard treatment for end-stage heart disease, heart transplantation is associated with acute cardiac rejection within 1 year of transplantation. The continuous application of immunosuppressants may cause side effects such as hepatic and renal toxicity, infection, and malignancy. Developing new pharmaceutical strategies to alleviate acute rejection after heart transplantation effectively and safely is of critical importance. In this study, we performed a murine model of MHC-full mismatch cardiac transplantation and showed that the combination of Rhodosin (Rho) and mycophenolate mofetil (MMF) could prevent acute rejection and oxidative stress injury and prolong the survival time of murine heart transplants. The use of Rho plus MMF in allografts improved the balance of Tregs/Teff cells, which had a protective effect on allotransplantation. We also isolated bone marrow-derived dendritic cells (BMDCs) and determined that Rho inhibited DC maturation by promoting mitochondrial fusion mainly through the mitochondrial fusion-related protein MFN1. Herein, we demonstrated that Rho, an active ingredient isolated from the plant with antioxidant and anti-inflammatory activities, could efficiently alleviate acute rejection and significantly prolong murine heart allograft survival when used with a low dose of MMF. More importantly, we found that Rho restrained DC maturation by promoting mitochondrial fusion and decreasing reactive oxygen species (ROS) levels, which then alleviated acute rejection in murine cardiac transplantation. Interestingly, as a novel immunosuppressant, Rho has almost no side effects compared with other traditional immunosuppressants. Taken together, these results suggest that Rho has good clinical auxiliary applications as an effective immunosuppressant and antioxidant, and this study provides an efficient strategy to overcome the side effects of immunosuppressive agents that are currently used in organ transplantation.
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http://dx.doi.org/10.1155/2022/7260305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288296PMC
July 2022

Electrochemical biosensor based on topological insulator BiSe tape electrode for HIV-1 DNA detection.

Mikrochim Acta 2022 07 19;189(8):285. Epub 2022 Jul 19.

Centre for Quantum Physics, Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement (MOE), School of Physics, Beijing Institute of Technology, Beijing, 100081, China.

A large-size BiSe tape electrode (BTE) was prepared by peeling off a 2 × 1 × 0.5 cm high-quality single crystal. The feasibility of using the flexible BTE as an efficient bioplatform to load Au nanoparticles and probe DNA for HIV-1 DNA electrochemical sensing was explored. Differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) show that the resultant biosensor has a wide linear range from 0.1 fM to 1 pM, a low detection limit of 50 aM, excellent selectivity, reproducibility and stability, and is superior to the pM DNA detection level of Pt-Au, graphene-AuNPs hybrid biosensors. This outstanding performance is attributed to the intrinsic surface state of BiSe topological insulator in facilitating electron transfer. Therefore, BTE electrochemical biosensor platform has great potential in the application for sensitive detection of DNA biomarkers.
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http://dx.doi.org/10.1007/s00604-022-05365-8DOI Listing
July 2022

Identification of Microproteins in under Different Stress Conditions.

J Proteome Res 2022 Aug 15;21(8):1939-1947. Epub 2022 Jul 15.

School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, Hubei 430079, People's Republic of China.

Small open reading frame-encoded peptides (SEPs) are microproteins with a length of 100 amino acids or less, which may play a critical role in maintaining cell homeostasis under stress. Therefore, we used mass spectrometry-based proteomics to explore microproteins potentially involved in cellular stress responses in . A total of 225 microproteins with 1920 unique peptides were identified under six culture conditions: normal, oxidation, starvation, ultraviolet radiation, heat shock, and heat shock with starvation. Among these microproteins, we found 70 SEPs with 75 unique peptides. The annotated microproteins are involved in stress-related processes, such as cell redox reactions, cell wall modification, protein folding and degradation, and DNA damage repair. It suggests that SEPs may also play similar functions under stress conditions. For example, SEP IP_008057, translated from a short coding sequence of , may play a role in heat shock. This study identified stress-responsive SEPs in and provided valuable information to determine the functions of these proteins, which enrich the genome and proteome of and show clues to improving the stress tolerance of .
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http://dx.doi.org/10.1021/acs.jproteome.2c00212DOI Listing
August 2022

Osteoid Osteoma of the Proximal Femur: Pitfalls in Diagnosis and Performance of Open Surgical Resection.

Front Surg 2022 28;9:922317. Epub 2022 Jun 28.

Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Aims: Proximal femoral osteoid osteoma (OO) is extremely easy to be misdiagnosed or missed. The purpose of this study was to retrospectively analyze the clinical data of patients with proximal femoral OO in order to determine the clinical manifestation and imaging characteristics of the disease, so as to provide help for the preoperative diagnosis and clinical treatment of proximal femoral OO.

Methods: This was a retrospective study involving 35 patients with proximal femoral OO admitted into our hospital from January 2015 to January 2021. The baseline characteristics of the participants included; 24 males and 11 females, aged between 13 and 25 (mean 16.2) years old, and the course of the disease was 1 to 14 (mean 6.3) months. We used previous medical experience records of the patients to analyze for the causes of misdiagnosis. Moreover, we compared the difference between preoperative and postoperative treatment practices in alleviating pain in OO patients and restoring hip function. Follow-ups were carried out regularly, and patients advised to avoid strenuous exercises for 3 months.

Results: We followed up 35 patients (25 intercortical, 4 sub-periosteal, and 6 medullary) for an average of 41.4 months. We found that 15 patients (42.9%) had been misdiagnosed of synovitis, perthes disease, osteomyelitis, intra-articular infection, joint tuberculosis and hip impingement syndrome, whose average time from symptoms to diagnosis were 6.3 months. Postoperative pain score and joint function score improved significantly compared with preoperative, and complications were rare.

Conclusion: Open surgical resection constitutes an effective treatment for proximal femoral OO by accurately and completely removing the nidus. Wrong choice of examination, and the complexity and diversity of clinical manifestations constitutes the main reasons for the misdiagnosis of proximal femoral OO.
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http://dx.doi.org/10.3389/fsurg.2022.922317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273932PMC
June 2022

Evidence of a room-temperature quantum spin Hall edge state in a higher-order topological insulator.

Nat Mater 2022 Jul 14. Epub 2022 Jul 14.

Laboratory for Topological Quantum Matter and Advanced Spectroscopy (B7), Department of Physics, Princeton University, Princeton, NJ, USA.

Room-temperature realization of macroscopic quantum phases is one of the major pursuits in fundamental physics. The quantum spin Hall phase is a topological quantum phase that features a two-dimensional insulating bulk and a helical edge state. Here we use vector magnetic field and variable temperature based scanning tunnelling microscopy to provide micro-spectroscopic evidence for a room-temperature quantum spin Hall edge state on the surface of the higher-order topological insulator BiBr. We find that the atomically resolved lattice exhibits a large insulating gap of over 200 meV, and an atomically sharp monolayer step edge hosts an in-gap gapless state, suggesting topological bulk-boundary correspondence. An external magnetic field can gap the edge state, consistent with the time-reversal symmetry protection inherent in the underlying band topology. We further identify the geometrical hybridization of such edge states, which not only supports the Z topology of the quantum spin Hall state but also visualizes the building blocks of the higher-order topological insulator phase. Our results further encourage the exploration of high-temperature transport quantization of the putative topological phase reported here.
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http://dx.doi.org/10.1038/s41563-022-01304-3DOI Listing
July 2022

LACC1 contributes to inflammation and cognitive disorder after stroke via the AMPK/NLRP3 pathway.

Acta Neurobiol Exp (Wars) 2022 ;82(2):207-212

Geriatrics Department, The Second Hospital of Hebei Medical University, Shi jiazhuang City, Hebei Province, China;

The current study aimed to investigate the effects of LACC1 on cognitive disorder due to stroke, as well as its underlying mechanism. LACC1 promoted inflammation and aggravated cognitive impairment in a mouse model of stroke. In an in vitro model of stroke, inhibition of LACC1 reduced inflammation and ROS‑induced oxidative stress by activating AMP‑activated protein kinase (AMPK) expression and suppressing NLPR3 expression. Furthermore, our studies revealed that inhibition of AMPK activity reduced the effects of si‑LACC1 on cognitive disorder in mice after stroke via the AMPK/NLPR3 pathway. AMPK activation also reduced the effects of LACC1 on inflammation and ROS‑induced oxidative stress via the NLPR3 pathway in the in vitro model that we evaluated. Our study suggests that LACC1‑aggravated inflammation causes cognitive impairment after stroke via the AMPK/NLRP3 pathway, which may provide a new therapeutic target for stroke and other neurological diseases and their associated complications. In sum, we identified an important role and regulatory mechanism for LACC1 in maintaining stroke‑induced cognitive disorder via the AMPK/NLRP3 pathway.
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http://dx.doi.org/10.55782/ane-2022-019DOI Listing
July 2022

Hyaluronic acid methacrylate/pancreatic extracellular matrix as a potential 3D printing bioink for constructing islet organoids.

Acta Biomater 2022 Jul 6. Epub 2022 Jul 6.

Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Nantong University, Nantong, 226006, China. Electronic address:

Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research. In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks. The HAMA/pECM hydrogel was validated in vitro to maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, which helps improve islet function and activity. Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation. In addition, the HAMA/pECM hydrogel can facilitate the attachment and growth of new blood vessels and increase the density of new vessels. Meanwhile, the designed 3D-printed structure was conducive to the formation of vascular networks and it promoted the construction of 3D-printed islet organoids. In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo. STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency. We developed a new tissue-specific bioink by combining pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA). The islet organoids constructed by 3D printing can mimic the microenvironment of the pancreas and maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, thereby improving islet function and activity. In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
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http://dx.doi.org/10.1016/j.actbio.2022.06.036DOI Listing
July 2022

Development of rapid low temperature assistant modified QuEChERS method for simultaneous determination of 107 pesticides and relevant metabolites in animal lipid.

Food Chem 2022 Nov 30;395:133606. Epub 2022 Jun 30.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/Key Laboratory of Detection for Pesticide Residues and Control of Zhejiang, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; Agricultural Ministry Key Laboratory for Pesticide Residue Detection, Hangzhou 310021, PR China. Electronic address:

A modified QuEChERS LC-MS/MS method was developed for 107 pesticides analysis in animal lipids such as pork lard, mutton tallow, chicken oil and butter. The challenges for high fat matrices clean up were studied in details by optimizing dispersive purification adsorbents coupled with rapid low temperature assistant methods. The method validation was carried out using pork lard and further appplied to other matrices by testing their recoveries. Good linearities were obtained with correlation coefficients greater than 0.99. Sensitive LOQs ranged from 5.0-50.0 μg kg. Both inter-day and intra-day precisions were lower than 20% indicating the good precision and accuracy of this method. The method applied to four animal lipids with 93%∼100% of analytes revealed satisfactory recoveries (ranged from 70% to 120%) and RSD (≤20%) at 10 µg kgand 50 µg kg spiking levels respectively related to the matrix.
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http://dx.doi.org/10.1016/j.foodchem.2022.133606DOI Listing
November 2022

Highly Selective Recovery of Phosphorus from Wastewater via Capacitive Deionization Enabled by Ferrocene-polyaniline-Functionalized Carbon Nanotube Electrodes.

ACS Appl Mater Interfaces 2022 Jul 8;14(28):31962-31972. Epub 2022 Jul 8.

State Key Laboratory of Pollution Control and Resource Reuse, Shanghai Institute of Pollution Control and Ecological Security, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.

While capacitive deionization (CDI) is a promising technology for the recovery of nutrients from wastewater, a selective recovery of phosphate from the wastewater containing high concentrations of competing ions is still a huge challenge. Herein, we reported a ferrocene-polyaniline-functionalized carbon nanotube (Fc-PANI/CNT) electrode prepared through amidation reaction and chemical oxidation polymerization, aiming for a highly selective recovery of phosphorus from wastewater. The Fc-PANI/CNT electrode with a unique structure and high conductivity could efficiently adsorb phosphate ions from complex synthetic wastewater with a nearly 100% selectivity, mainly because the integration of ferrocene and an amide bond in Fc-PANI resulted in an enhanced charge transfer (Faradaic reactions) and a strong hydrogen bonding interaction with phosphate ions in its oxidized state. Density functional theory calculations showed that the binding energies of the oxidized Fc-PANI with HPO and HPO were much greater than those of the oxidized Fc-PANI with other competing anions. The affinity of Fc-PANI/CNTs with phosphate can be controlled electrochemically based on the synergetic effects of Faradaic reactions and hydrogen bonding, enabling a selective recovery of phosphate through charging/discharging cycles. The phosphate adsorption capacity reached up to 35 mg PO g in a NaCl/NaSO/NaNO/NaHPO complex mixture at 1.2 V, outperforming most of the other reported CDI systems. The Fc-PANI/CNT electrode also exhibited a decent regeneration ability and durability during repeated CDI tests, demonstrating a great potential for the application of selective recovery and enrichment of phosphate from wastewater.
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http://dx.doi.org/10.1021/acsami.2c06248DOI Listing
July 2022

MiR-487b suppressed inflammation and neuronal apoptosis in spinal cord injury by targeted Ifitm3.

Metab Brain Dis 2022 Jul 8. Epub 2022 Jul 8.

Department of Orthopedics, Changhai Hospital, Naval Medical University, 168 Changhai Road, Shanghai, 200433, People's Republic of China.

Spinal cord injury (SCI) was a serious nerve injury, which involves complex genetic changes. This paper was intended to investigate the function and mechanism of differentially expressed genes in SCI. The three datasets GSE92657, GSE93561 and GSE189070 of SCI from GEO database were used to identify differentially expressed genes (DEGs). We identified the common DEGs in the three datasets GSE92657, GSE93561 and GSE189070 of SCI from GEO database. Next, a protein-protein interaction (PPI) network of DEGs was constructed. Subsequently, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEGs were significantly enriched in immune response, inflammatory response. The expression level of immune-related genes (Arg1, Ccl12, Ccl2, Ifitm2, Ifitm3, and et al.) at different time points of SCI were analyzed in GSE189070 dataset. Next, differentially expressed miRNAs (DE-miRNAs) were identified in SCI compared with normal based on GSE158194 database. DE-miRNA and targeted immune-related genes were predicted by miRwalk, including miR-487b-5p targeted Ifitm3, miR-3072-5p targeted Ccl3, and et al. What's more, the miR-487b was identified and verified to be down-regulated in Lipopolysaccharide (LPS)-induced BV-2 cell model. Further, the miR-487b inhibited cell inflammation and apoptosis in LPS-induced BV2 cell by targeted Ifitm3. For the first time, our results revealed that miR-487b may play an important regulatory role in SCI by targeted Ifitm3 and provide further evidence for SCI research.
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http://dx.doi.org/10.1007/s11011-022-01015-3DOI Listing
July 2022

A Room-Temperature Ferroelectric Resonant Tunneling Diode.

Adv Mater 2022 Jul 8:e2205359. Epub 2022 Jul 8.

School of Materials Science and Engineering, University of New South Wales, Sydney, 2052, Australia.

Resonant tunneling is a quantum-mechanical effect in which electron transport is controlled by the discrete energy levels within a quantum-well (QW) structure. A ferroelectric resonant tunneling diode (RTD) exploits the switchable electric polarization state of the QW barrier to tune the device resistance. Here, the discovery of robust room-temperature ferroelectric-modulated resonant tunneling and negative differential resistance (NDR) behaviors in all-perovskite-oxide BaTiO /SrRuO /BaTiO QW structures is reported. The resonant current amplitude and voltage are tunable by the switchable polarization of the BaTiO ferroelectric with the NDR ratio modulated by ≈3 orders of magnitude and an OFF/ON resistance ratio exceeding a factor of 2 × 10 . The observed NDR effect is explained an energy bandgap between Ru-t and Ru-e orbitals driven by electron-electron correlations, as follows from density functional theory calculations. This study paves the way for ferroelectric-based quantum-tunneling devices in future oxide electronics.
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http://dx.doi.org/10.1002/adma.202205359DOI Listing
July 2022

The Development and Progression of Micro-Nano Optics.

Front Chem 2022 20;10:916553. Epub 2022 Jun 20.

Laboratory of Optical Detection and Imaging, School of Science, Qingdao University of Technology, Qingdao, China.

Micro-Nano optics is one of the most active frontiers in the current development of optics. It combines the cutting-edge achievements of photonics and nanotechnology, which can realize many brand-new functions on the basis of local electromagnetic interactions and become an indispensable key science and technology of the 21st century. Micro-Nano optics is also an important development direction of the new optoelectronics industry at present. It plays an irreplaceable role in optical communication, optical interconnection, optical storage, sensing imaging, sensing measurement, display, solid-state lighting, biomedicine, security, green energy, and other fields. In this paper, we will summarize the research status of micro-nano optics, and analyze it from four aspects: micro-nano luminescent materials and devices, micro-nano optical waveguide materials and devices, micro-nano photoelectric detection materials and devices, and micro-nano optical structures and devices. Finally, the future development of micro-nano optics will be prospected.
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http://dx.doi.org/10.3389/fchem.2022.916553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251314PMC
June 2022

Plant growth and heavy meal accumulation characteristics of cultured in three soil extractions with and without silicate supplementation.

Int J Phytoremediation 2022 Jul 5:1-14. Epub 2022 Jul 5.

Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, China.

A hydroponic method was conducted to test whether is tolerant to multiple HMs as well as to evaluate whether sodium silicate promotes plant growth and alleviates HM stress mainly by assessing biomass, HM accumulation characteristics and antioxidant enzyme activities (AEAs). Three soil extractions from an uncontaminated soil, a comparable lightly HM-contaminated soil (EnSE), and a comparable heavily HM-contaminated soil (ExSE) with or without 1 mM sodium silicate supplementation were used. showed no obvious symptoms when cultured in EnSE and ExSE, indicating that it was a multi-HM-tolerant species. The biomass and photosynthesis followed the order: UnSE > EnSE > ExSE, but the opposite order was found for HM concentration, AEAs, and malondialdehyde content. Silicate had no effects on the growth and HM bioaccumulation characteristics of cultured in UnSE but exhibited a novel role in decreasing HM uptake by 13.61-41.51% in EnSE and ExSE, respectively, corresponding upregulated AEAs, and reduced malondialdehyde contents, resulting in increased biomass and alleviating HM stress. The activities of peroxidase and superoxide dismutase were upregulated by an increase in soil extraction HM concentration and further upregulated by silicate supplementation, indicating that they were important mechanisms alleviating HM stress in .
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http://dx.doi.org/10.1080/15226514.2022.2092059DOI Listing
July 2022

Chiral perspective evaluations: Enantioselective hydrolysis of 6PPD and 6PPD-quinone in water and enantioselective toxicity to Gobiocypris rarus and Oncorhynchus mykiss.

Environ Int 2022 Jun 27;166:107374. Epub 2022 Jun 27.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/ Key Laboratory of Detection for Pesticide Residues and Control of Zhejiang, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China; Agricultural Ministry Key Laboratory for Pesticide Residue Detection, Hangzhou 310021, PR China. Electronic address:

As a ubiquitous tire antidegradant, N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD) is persistently released into the environment. It is highly toxic to aquatic organisms, and its transformation product 6PPD-quinone (6PPD-Q), is "very highly toxic" to Oncorhynchus kisutch at a median lethal concentration (LC) of < 0.1 ng/mL. Notably, 6PPD and 6PPD-Q are chiral compounds. Here, enantioselective evaluations, including hydrolysis and acute toxicity were conducted after preparing the enantiomer, confirming the enantiomer absolute configuration and establishing enantioseparation methods. In the 6PPD hydrolysis experiments, the products 6PPD-Q, phenol, 4-[(1,3-dimethylbutyl)amino]- (4-DBAP) and 4-hydroxydiphenylamine (4-HDPA) were detected. In different water solutions, the hydrolysis of 4-DBAP and 4-HDPA was very fast (0.87-107 h), while the 6PPD-Q hydrolysis half-lives (12.8-16.3 d) were significantly longer than 6PPD (4.83-64.1 h). At the enantiomeric level, no enantioselective hydrolysis and conversion occurred. R-6PPD generated R-6PPD-Q, and S-6PPD generated S-6PPD-Q, and the formation rate of S-6PPD-Q was 1.77 times faster than R-6PPD-Q. In terms of the enantioselective toxicity, the 6PPD enantiomer was highly toxic to China-specific Gobiocypris rarus (LC, 162-201 ng/mL), and it had no enantioselective difference. 6PPD-Q was "very highly toxic" (LC, 1.66-4.31 ng/mL) to Oncorhynchus mykiss, which is of commercial importance, and the toxicities of rac-6PPD-Q and S-6PPD-Q were 1.9 and 2.6 times higher than R-6PPD-Q. Furthermore, the formation concentrations of S-6PPD-Q and R-6PPD-Q in 6PPD water solutions were higher than the LC values of O. kisutch and O. mykiss, and the toxicity of 6PPD-Q was highly species-specific, which should raise concern. These results provide important information for environmental risk assessments of 6PPD and 6PPD-Q, especially from the perspective of enantiomers.
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http://dx.doi.org/10.1016/j.envint.2022.107374DOI Listing
June 2022

Tunable fluorescent amino-functionalized TiCT MXene quantum dots for ultrasensitive Fe ion sensing.

Nanoscale 2022 Jul 7;14(26):9498-9506. Epub 2022 Jul 7.

Institute for Advanced Study, Shenzhen University, Shenzhen, 518060, China.

The development of sensors with high sensitivity, good selectivity and reproducibility are of great importance for the detection of Fe in contaminated water for environmental monitoring. In this work, a reflux approach has been adopted to synthesize TiCT quantum dots (QDs) based on the cutting effect of tetramethylammonium hydroxide (TMAOH) on TiCT at high temperature. The surface-functionalized TiCT QDs contained abundant amino groups and exhibited tunable pH-dependent emission, which was attributed to the protonation and deprotonation of the surface terminations. The linearity of the radiometric fluorescence intensity pH indicates its great potential as a dual-emission ratiometric pH sensor. Additionally, the TiCT QDs exhibited tunable excitation-dependent emission behavior, which was related to the degree of passivation by the amino groups on the surface. Furthermore, the fluorescence intensity of the TiCT QDs shows a linear response toward Fe in the nanomolar to micromolar range with a low detection limit of 2 nM, originating from the oxidation and reduction between Fe and TiCT. This ultra-sensitive and selective detection capability demonstrated the environmental application potential for TiCT QDs as a nanoprobe to monitor Fe.
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http://dx.doi.org/10.1039/d2nr02088bDOI Listing
July 2022

Integrated Transcriptomic and Metabolomic Analyses of Cold-Tolerant and Cold-Sensitive Pepper Species Reveal Key Genes and Essential Metabolic Pathways Involved in Response to Cold Stress.

Int J Mol Sci 2022 Jun 15;23(12). Epub 2022 Jun 15.

Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, College of Horticulture, Hainan University, Haikou 570228, China.

Cold stress, triggered by particularly low temperatures, is one of the most severe forms of abiotic stress in pepper plants and a major constraint to the global pepper industry, threatening crop production and food security. To acclimatize to extreme conditions, the plant undergoes numerous modifications, including genetic and metabolic modulations. A thorough study of both the genetic and metabolic alterations of plants in response to cold stress is vital to understanding and developing the cold stress resistance mechanism. This study implemented transcriptome and metabolome analyses to evaluate the cold stress response in cold-tolerant and cold-sensitive pepper species. The weighted gene co-expression network revealed three significant modules related to cold stress tolerance in . We identified 17 commonly enriched genes among both species at different time points in 10 different comparisons, including the transcription factor, LRR receptor-like serine, hypersensitivity-related 4-like protein, and uncharacterized novel.295 and novel.6172 genes. A pathway enrichment analysis indicated that these DEGs were mainly associated with the MAPK signaling pathway, hormone signaling pathway, and primary and secondary metabolism. Additionally, 21 significantly differentially accumulated metabolites (DAMs) were identified in both species after 6 h of cold stress. A transcriptome and metabolome integrated analysis revealed that 54 genes correlated with metabolites enriched in five different pathways. Most genes and metabolites involved in carbohydrate metabolism, the TCA cycle, and flavonoid biosynthesis pathways were upregulated in cold-tolerant plants under cold stress. Together, the results of this study provide a comprehensive gene regulatory and metabolic network in response to cold stress and identified some key genes and metabolic pathways involved in pepper cold tolerance. This study lays a foundation for the functional characterization and development of pepper cultivars with improved cold tolerance.
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http://dx.doi.org/10.3390/ijms23126683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224482PMC
June 2022

Perioperative Fluoroquinolone Treatment Deteriorates Prognosis Following Coronary Artery Bypass Grafting.

J Cardiovasc Dev Dis 2022 May 28;9(6). Epub 2022 May 28.

Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, China.

Background: Former studies have revealed that fluoroquinolone (FQ) can induce aortic expansion and rupture. While FQ is widely used in perioperative anti-infection therapy, its impact on graft patency and patient survival is unknown.

Methods: Coronary artery bypass grafting (CABG) data were extracted from the MIMIC-III database. Chi-square tests, Fisher's exact tests, -tests, or ANOVAs were used to compare baseline data between groups determined by FQ therapy status, depending on the data type. Propensity score matching was used to establish a balanced cohort. Cox regression was used to investigate the impact of FQ on CABG patient survival, whereas paired -tests were used to analyze secondary results.

Results: Of the 5030 patients who underwent CABG, 937 (18.6%) received oral or intravenous FQ therapy. Using propensity score matching, these 819 patients were successfully matched with 819 controls in a 1:1 ratio. Cox regression showed that FQ significantly decreased survival among CABG patients (HR: 1.62, 95% CI: 1.21-2.15, = 0.001). Furthermore, FQ usage was associated with longer hospitalization (<0.0001), ICU duration (<0.0001), ventilation period (<0.0001), and duration of vasopressor administration (<0.0001).

Conclusions: Perioperative FQ therapy was associated with worse prognosis and a more difficult recovery among patients with CABG.
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http://dx.doi.org/10.3390/jcdd9060173DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224777PMC
May 2022
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