Publications by authors named "Zhiwei Liu"

390 Publications

Immunologic markers and risk of hepatocellular carcinoma in hepatitis B virus- and hepatitis C virus-infected individuals.

Aliment Pharmacol Ther 2021 Jul 19. Epub 2021 Jul 19.

Genomics Research Center, Academia Sinica, Taipei, Taiwan.

Background: Clinical and experimental studies suggest immunologic proteins contribute to hepatocellular carcinoma (HCC) development.

Aim: To evaluate circulating immunologic markers and HCC risk.

Methods: From a Taiwanese cohort of chronically hepatitis B virus (HBV)-infected individuals followed over time (REVEAL-HBV), we sampled 175 who developed HCC, 117 cirrhosis only, and 165 non-cirrhotic controls. From a similar Taiwanese cohort of chronically hepatitis C virus (HCV)-infected individuals (REVEAL-HCV), we included 94 individuals who developed HCC, 68 cirrhosis only and 100 non-cirrhotic controls. We compared pre-diagnostic plasma levels of 102 markers in HCC cases to non-cirrhotic and cirrhotic controls using polytomous logistic regression. A priori markers included insulin-like growth factor binding protein-3 (IGFBP-3), intercellular adhesion molecule 1 (ICAM-1) and interleukin 6 (IL-6). P-values for other markers were corrected for multiple testing (false discovery rate = 10%).

Results: In both REVEAL-HBV and REVEAL-HCV, increasing levels of ICAM-1 were associated with increased risk of HCC compared to non-cirrhotic controls (P-trend 0.02 and 0.001, respectively). In both REVEAL-HBV and REVEAL-HCV, two novel markers [C-X-C motif chemokine 11 (CXCL11) and hepatocyte growth factor (HGF)] were positively associated [strongest odds ratio (OR) 4.55 for HGF in HCV], while two [complement factor H related 5 (CFHR5) and stem cell factor (SCF)] were negatively associated (strongest OR 0.14 for SCF in HCV) with development of HCC compared to non-cirrhotic controls.

Conclusions: We confirmed the association for ICAM-1 and identified 4 additional proteins associated with HBV- and HCV-related HCC. These findings highlight the importance of immunologic processes in HBV- and HCV-related HCC.
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http://dx.doi.org/10.1111/apt.16524DOI Listing
July 2021

Strategies to Attenuate Myocardial Infarction and No-Reflow through Preservation of Vascular Integrity by Pigment Epithelium-derived Factor.

Hum Gene Ther 2021 Jul 18. Epub 2021 Jul 18.

Xuzhou Medical University, 38044, Tongshan 209 road, xuzhou medical university, xuzhou, jiangsu, XUZHOU, Jiangsu, China, 221001;

The phenomenon of no-reflow seriously limits the therapeutic value of coronary recanalization and leads to poor prognosis. Recent studies have demonstrated the potential role of PEDF in stabilizing endothelial cell junction, reducing vascular permeability and maintaining a quiescent vasculature. Here, intramyocardial gene delivery was performed 5 days prior to the AMI/R experiment in male rats. Positron emission tomography (PET) perfusion imaging with 13N-NH3 indicated PEDF to promote microvascular reperfusion significantly 4 hours post coronary occlusion. PEDF was observed to maintain the stability of endothelial adhesion junctions (AJs), thus preventing the occurrence of no-reflow. PEDF reduced the hypoxia-induced VE-cadherin endocytosis through PEDF/LR/Src/VE-cadherin S665 axis in vitro, which was remarkably observed to maintain endothelial AJs. Generally, PEDF might function as a relevant target for therapeutic vasculoprotection by way of regulating the phosphorylation level of VE-cadherin according to our data, thus being crucial for preventing no-reflow.
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http://dx.doi.org/10.1089/hum.2021.068DOI Listing
July 2021

Patterns of antipsychotic prescriptions in patients with schizophrenia in China: A national survey.

Asian J Psychiatr 2021 Aug 26;62:102742. Epub 2021 Jun 26.

Department of Psychiatry, Chaohu Hospital of Anhui Medical University, 64 Chaohu North Road, Chaohu District, Hefei, China; Department of Psychiatry, Anhui Psychiatric Center, 64 Chaohu North Road, Chaohu District, Hefei, China. Electronic address:

Introduction: To investigate the patterns and correlates of antipsychotic prescriptions among recently discharged inpatients with schizophrenia in China.

Methods: The study included discharged patients from 41 tertiary psychiatric hospitals in 29 provinces between March 19-30, 2019. A total of 1032 inpatients with schizophrenia were included. Socio-demographic and clinical data were retrieved from medical records upon discharge.

Results: Patients received a total of 13 unique antipsychotic medications, which included 9 s-generation antipsychotics (SGAs) and 4 first-generation antipsychotics (FGAs). The utilization rates of SGAs and FGAs were 98.8 % and 6.1 % respectively. The three most commonly antipsychotic medications were risperidone (35.1 %), olanzapine (31.3 %), and clozapine (24.6 %). The mean chlorpromazine equivalent dose was 452.12 ± 230.74 mg/day. The utilization rate of mood stabilizers was 18.9 %, 8.8 % for antidepressants, 20.3 % for sleep improvers, and 9.9 % for anticholinergics. More than two fifths patients (43.1 %) received two or more antipsychotic medications. Predictors of antipsychotic polypharmacy included younger age, residing in Central or West China, a longer duration of illness, a history of prior hospitalizations, and having agitated behavior during the hospitalization.

Conclusion: Antipsychotic polypharmacy in China is common on inpatients settings. The proportion of antipsychotic polypharmacy in China is higher than in many other countries, despite limited data to support the efficacy of many combinations. Clozapine remains one of most commonly prescribed antipsychotics in China, either as a monotherapy or combination therapy.
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http://dx.doi.org/10.1016/j.ajp.2021.102742DOI Listing
August 2021

Sex Differences in the Association of HOMA-IR Index and BDNF in Han Chinese Patients With Chronic Schizophrenia.

Front Psychiatry 2021 21;12:656230. Epub 2021 Jun 21.

Department of Psychiatry, Chaohu Hospital of Anhui Medical University, Chaohu, China.

Previous research has indicated that there are significant sex differences in serum BDNF levels and metabolic indicators in patients with schizophrenia. Studies have found that BDNF is involved in blood sugar regulation. Homeostasis model assessment of insulin resistance (HOMA-IR) is currently a sensitive indicator for measuring insulin resistance. Our study aims to explore the sex differences in the relationship between serum BDNF levels and HOMA-IR in patients with chronic schizophrenia (CS). A total of 332 patients with CS were enrolled in this study. General information of all participants was collected. Haematological indicators were collected, and the Positive and Negative Syndrome Scale (PANSS) was used to evaluate psychiatric symptoms. Sex differences in serum BDNF levels, HOMA-IR index and other metabolic indexes were investigated. Then, linear regression analysis was used to analyse the relationship between the HOMA-IR index and BDNF levels in male and female patients. The HOMA-IR index of female patients was significantly higher than that of males, but there was no significant difference in serum BDNF levels between male patients and female patients. There was a positive correlation between BDNF level and HOMA-IR index, and this relationship only existed in female patients. The results show that there are significant sex differences in HOMA-IR in patients with CS. In addition, only in female patients was there a positive correlation between the HOMA-IR index and BDNF level, which suggests that sex factors should be taken into account in evaluating the relationship between BDNF and blood glucose in patients with CS.
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http://dx.doi.org/10.3389/fpsyt.2021.656230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255609PMC
June 2021

Comparison of new magnetic resonance imaging grading system with conventional endoscopy for the early detection of nasopharyngeal carcinoma.

Cancer 2021 Jul 7. Epub 2021 Jul 7.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, Maryland, USA.

Background: Although stratifying individuals with respect to nasopharyngeal carcinoma (NPC) risk with Epstein-Barr virus-based markers is possible, the performance of diagnostic methods for detecting lesions among screen-positive individuals is poorly understood.

Methods: The authors prospectively evaluated 882 participants aged 30 to 70 years who were enrolled between October 2014 and November 2018 in an ongoing, population-based NPC screening program and had an elevated NPC risk. Participants were offered endoscopy and magnetic resonance imaging (MRI), and lesions were identified either by biopsy at a follow-up endoscopy or further contact and linkage to the local cancer registry through December 31, 2019. The diagnostic performance characteristics of endoscopy and MRI for NPC detection were investigated.

Results: Eighteen of 28 identified NPC cases were detected by both methods, 1 was detected by endoscopy alone, and 9 were detected by MRI alone. MRI had significantly higher sensitivity than endoscopy for NPC detection overall (96.4% vs 67.9%; P = .021) and for early-stage NPC (95.2% vs 57.1%; P = .021). The sensitivity of endoscopy was suggestively lower among participants who had previously been screened in comparison with those undergoing an initial screening (50.0% vs 81.2%; P = .11). The authors observed a higher overall referral rate by MRI versus endoscopy (17.3% vs 9.1%; P < .001). Cases missed by endoscopy had early-stage disease and were more commonly observed for tumors originating from the pharyngeal recess.

Conclusions: MRI was more sensitive than endoscopy for NPC detection in the context of population screening but required the referral of a higher proportion of screen-positive individuals. The sensitivity of endoscopy was particularly low for individuals who had previously been screened.
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http://dx.doi.org/10.1002/cncr.33552DOI Listing
July 2021

Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.

Cell Death Differ 2021 Jul 2. Epub 2021 Jul 2.

Chemical Proteomics Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

SKP1-CUL1-F-box (SCF) ubiquitin ligases play fundamental roles in cellular functions. Typically, substrate phosphorylation is required for SCF recognition and subsequent degradation. However, phospho-dependent substrates remain largely unidentified. Here, using quantitative phoshoproteome approach, we performed a system-wide investigation of phospho-dependent SCF substrates. This strategy identified diverse phospho-dependent candidates. Biochemical verification revealed a mechanism by which SCF recognizes the motif XXPpSPXPXX as a conserved phosphodegron to target substrates for destruction. We further demonstrated BAG3, a HSP70 co-chaperone, is a bona fide substrate of SCF. FBXO22 mediates BAG3 ubiquitination and degradation that requires ERK-dependent BAG3 phosphorylation at S377. FBXO22 depletion or expression of a stable BAG3 S377A mutant promotes tumor growth via defects in apoptosis and cell cycle progression in vitro and in vivo. In conclusion, our study identified broad phosphorylation-dependent SCF substrates and demonstrated a phosphodegron recognized by FBXO22 and a novel ERK-FBXO22-BAG3 axis involved in tumorigenesis.
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http://dx.doi.org/10.1038/s41418-021-00827-7DOI Listing
July 2021

Construction of Multifunctional and Adjustable Langmuir-Blodgett Composite Films Containing Black Phosphorus with High Stability for Optically Electrical Applications.

Langmuir 2021 Jul 2;37(28):8616-8626. Epub 2021 Jul 2.

State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, P. R. China.

Fabrication of composite thin-film materials based on black phosphorus (BP) will greatly broaden the applications of BP in various areas. However, it is still a challenge to prepare a BP-based composite film with good stability and controllable structure. In this work, a series of BP-based composite Langmuir-Blodgett (LB) films are prepared by the self-assembly of polyethyleneimine (PEI)-modified BP nanosheets (BPNSs) (BPNS-PEI) and dye molecules. The presence of PEI greatly improves the stability of BPNSs. As for BPNS-PEI and dye molecules, the electrostatic interactions or π-π stacking interactions ensure the formation of stable composite LB films. Due to the protonation and deprotonation of amino groups, the synthesized BPNS-PEI/dye composite films show a sensitive response to acid and alkali gases, which shows wide application prospects as a highly sensitive gas sensor. Furthermore, surface-enhanced Raman scattering (SERS) proves that the prepared LB films exhibit good reproducibility and obvious Raman enhancement effect on rhodamine 6G molecules. In addition, due to the high carrier transfer rate of the obtained composite films, they possess enhanced photocurrent generation performance than pure BPNS-PEI and pure dye films. The current work demonstrates an effective method for preparing the ordered self-assembled BP-based composite LB films with good SERS and photoelectric conversion performance.
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http://dx.doi.org/10.1021/acs.langmuir.1c01260DOI Listing
July 2021

Comparison of Different Methods for Extracting the Astaxanthin from : Chemical Composition and Biological Activity.

Molecules 2021 Jun 11;26(12). Epub 2021 Jun 11.

College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.

In this study, the impact of different cell disruption techniques (high-pressure micro fluidization (HPMF), ionic liquids (ILs), multi-enzyme (ME), and hydrochloric acid (HCl)) on the chemical composition and biological activity of astaxanthin (AST) obtained from was investigated. Results indicated that all cell disruption techniques had a significant effect on AST composition, which were confirmed by TLC and UPC analysis. AST recovery from HCl (HCl-AST) and ILs (ILs-AST) cell disruption techniques was dominant by free and monoesters AST, while AST recovery from HPMF (HPMF-AST) and ME (ME-AST) cell disruption techniques was composed of monoesters, diesters, and free AST. Further biological activity analysis displayed that HCl-AST showed the highest ABTS and DPPH activity, while ILs-AST showed better results against the ORAC assay. Additionally, ILs-AST exhibits a stronger anti-proliferation of HepG2 cells in a dose-dependent manner, which was ascribed to AST-induced ROS in to inhibit the proliferative of cancer cells.
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http://dx.doi.org/10.3390/molecules26123569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230668PMC
June 2021

An Electrostatic Self-Excited Resonator with Pre-Tension/Pre-Compression Constraint for Active Rotation Control.

Micromachines (Basel) 2021 Jun 1;12(6). Epub 2021 Jun 1.

School of Energy and Power Engineering, Beihang University, Beijing 100191, China.

We report a novel electrostatic self-excited resonator driven by DC voltage that achieves variable velocity-position characteristics via applying the pre-tension/pre-compression constraint. The resonator consists of a simply supported micro-beam, two plate electrodes, and two adjustable constraint bases, and it can be under pre-compression or pre-tension constraint by adjusting the distance between two constraint bases (when beam length > , the resonator is under pre-compression and when < , it is under pre-tension). The oscillating velocity of the beam reaches the maximum value in the position around electrodes under the pre-compression constraint and reaches the maximum value in the middle position between two electrodes under the pre-tension condition. By changing the constraint of the microbeam, the position of the maximum velocity output of the oscillating beam can be controlled. The electrostatic self-excited resonator with a simple constraint structure under DC voltage has great potential in the field of propulsion of micro-robots, such as active rotation control of flapping wings.
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http://dx.doi.org/10.3390/mi12060650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227978PMC
June 2021

Portable, Rapid, and Sensitive Time-Resolved Fluorescence Immunochromatography for On-Site Detection of Dexamethasone in Milk and Pork.

Foods 2021 Jun 10;10(6). Epub 2021 Jun 10.

Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.

Dexamethasone (DEX) is widely used because of its anti-inflammatory, anti-endotoxin, anti-shock, and stress-enhancing response activities. It can increase the risk of diabetes and hypertension if it is abused or used improperly. However, there is a lack of sensitive and rapid screening methods for DEX in food. In this study, a time-resolved fluorescent microspheres immunochromatographic assay (TRFM-ICA) integrated with a portable fluorescence reader was developed for the quantitative detection of DEX in milk and pork. The cut-off values of the TRFM-ICA were 0.25 ng/mL and 0.7 µg/kg, respectively. The limits of quantitation (LOQs) were 0.003 ng/mL and 0.062 µg/kg, respectively. The recovery rates were 80.0-106.7%, and 78.6-83.6%, respectively, with the coefficients of variation ranging 6.3-12.5%, and 7.5-10.3%, respectively. A parallel experiment for 20 milk and 10 pork samples with LC-MS/MS was carried out to confirm the performance of the on-site application of the developed TRFM-ICA. The results of the two methods are basically the same; the correlation (R) was >0.98. The establishment of TRFM-ICA will provide a new sensitive and efficient technical support for the rapid screening of DEX in food.
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http://dx.doi.org/10.3390/foods10061339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229924PMC
June 2021

Roles of vascular endothelial and smooth muscle cells in the vasculoprotective effect of insulin in a mouse model of restenosis.

Diab Vasc Dis Res 2021 May-Jun;18(3):14791641211027324

Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.

Background: Insulin exerts vasculoprotective effects on endothelial cells (ECs) and growth-promoting effects on vascular smooth muscle cells (SMCs) in vitro, and suppresses neointimal growth in vivo. Here we determined the role of ECs and SMCs in the effect of insulin on neointimal growth.

Methods: Mice with transgene CreER under the control of EC-specific Tie2 (Tie2-Cre) or SMC-specific smooth muscle myosin heavy chain promoter/enhancer (SMMHC-Cre) or littermate controls were crossbred with mice carrying a loxP-flanked insulin receptor (IR) gene. After CreER-loxP-mediated recombination was induced by tamoxifen injection, mice received insulin pellet or sham (control) implantation, and underwent femoral artery wire injury. Femoral arteries were collected for morphological analysis 28 days after wire injury.

Results: Tamoxifen-treated mice showed lower IR expression in ECs, but not in SMCs, than mice. Insulin treatment reduced neointimal area after arterial injury in mice, but had no effect in mice. Tamoxifen-treated mice showed lower IR expression in SMCs, but not in ECs, than mice. Insulin treatment reduced neointimal area in mice, whereas unexpectedly, it failed to inhibit neointima formation in mice.

Conclusion: Insulin action in both ECs and SMCs is required for the "anti-restenotic" effect of insulin in vivo.
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http://dx.doi.org/10.1177/14791641211027324DOI Listing
June 2021

Dynamic anti-plane behavior of rare earth giant magnetostrictive medium with a circular cavity defect in semi-space.

Authors:
Zhiwei Liu Hui Qi

Sci Rep 2021 Jun 29;11(1):13442. Epub 2021 Jun 29.

College of Aerospace and Civil Engineering, Harbin Engineering University, Harbin, China.

An analytical solution to the anti-plane dynamics problem of semi-space rare earth giant magnetostrictive media with circular cavity defects near the horizontal boundary under the action of SH wave is studied. Based on the Helmholtz theorem and the theory of complex function, the elastic-magnetic dynamic equation of magnetostrictive medium is established, and the semi-space incident wave field is written. In addition, based on the theory of complex function and the method of wave function expansion, the expression of the wave function of the scattered displacement field and the corresponding magnetic potential of the scattered wave under the condition of no stress and magnetic insulation of the horizontal boundary are obtained. Then, based on the conditions of free boundary stress, continuous magnetic induction intensity and continuous magnetic potential around the circular cavity, the infinite linear algebraic equations are established. Finally, the analytical expressions of dynamic stress concentration factor and magnetic field intensity concentration factor around circular cavity in semi-space rare earth giant magnetostrictive medium are obtained. Numerical examples show that the analysis results depend on the following parameters: permeability, dimensional-piezomagnetic coefficient, frequency of the incident wave, incident angle, distance between the circular cavity and horizontal boundary. These results have certain reference value for the study of non-destructive testing and failure analysis of rare earth giant magnetostrictive materials.
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http://dx.doi.org/10.1038/s41598-021-92841-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241854PMC
June 2021

How to not build a cage: endohedral functionalization of polyoxometalate-based metal-organic polyhedra.

Chem Sci 2021 Apr 3;12(21):7361-7368. Epub 2021 Apr 3.

MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology Harbin 150001 China

Introducing functionalities into the interior of metal-organic cage complexes can confer properties and utilities ( catalysis, separation, drug delivery, and guest recognition) that are distinct from those of unfunctionalized cages. Endohedral functionalization of such cage molecules, for decades, has largely relied on modifying their organic linkers to covalently append targeted functional groups to the interior surface. We herein introduce an effective coordination method to bring in functionalities at the metal sites instead, for a set of polyhedral cages where the nodes are formed polyoxovanadate clusters, [V O(OCH)(μ-SO)(COO)]. Replacing the central sulfates of these hexavanadate clusters with more strongly coordinating phosphonate groups allows the installation of functionalities within the cage cavities. Organophosphonates with phenyl, biphenyl, and terphenyl tails were examined for internalization. Depending on the size/shape of the cavities, small phosphonates can fit into the molecular containers whereas larger ones inhibit or transform the framework architecture, whereby the first non-cage complex was isolated from a reaction that otherwise would lead to entropically favored regular polyhedra cages. The results highlight the complex and dynamic nature of the self-assembly process involving polyoxometalates and the scope of molecular variety accessible by the introduction of endo functional groups.
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http://dx.doi.org/10.1039/d1sc01243fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171318PMC
April 2021

Deep learning wavefront sensing method for Shack-Hartmann sensors with sparse sub-apertures.

Opt Express 2021 May;29(11):17669-17682

In this letter, we proposed a deep learning wavefront sensing approach for the Shack-Hartmann sensors (SHWFS) to predict the wavefront from sub-aperture images without centroid calculation directly. This method can accurately reconstruct high spatial frequency wavefronts with fewer sub-apertures, breaking the limitation of d/r ≈ 1 (d is the diameter of sub-apertures and r is the atmospheric coherent length) when using SHWFS to detect atmospheric turbulence. Also, we used transfer learning to accelerate the training process, reducing training time by 98.4% compared to deep learning-based methods. Numerical simulations were employed to validate our approach, and the mean residual wavefront root-mean-square (RMS) is 0.08λ. The proposed method provides a new direction to detect atmospheric turbulence using SHWFS.
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http://dx.doi.org/10.1364/OE.427261DOI Listing
May 2021

Ether-linked porphyrin covalent organic framework with broadband optical switch.

iScience 2021 Jun 11;24(6):102526. Epub 2021 May 11.

Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.

It is still a challenge to design and synthesize novel switchable optical materials with ultrafast nonlinear optical (NLO) response in a broad spectral range. These materials have exhibited great application potential in many high-technology fields such as biological imaging, chemical sensors, optical data storage, laser protection, and controllable intelligent and optoelectronic devices. By using porphyrins with highly delocalized 18 π-electron conjugated system as functional building blocks, the first ether-linked porphyrin covalent organic framework materials (COF-Pors) with highly ordered lattice structure have been successfully synthesized. In contrast to the starting porphyrins that only exhibit reverse saturable absorption (RSA) response at 532 nm, the as-prepared COF-Pors shows large NLO effect in a broad range from visible to near infrared. Upon laser illumination, COF-Pors exhibits typical saturable absorption (SA) effect at lower incident laser energy, and RSA response at higher pulse energy.
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http://dx.doi.org/10.1016/j.isci.2021.102526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188477PMC
June 2021

Effects of seated lumbar rotation manipulation in treating degenerative lumbar instability: a protocol for a randomized controlled trial.

Trials 2021 Jun 14;22(1):398. Epub 2021 Jun 14.

Department of Spine, Wangjing Hospital of China Academy of Chinese Medical Sciences, Beijing, 100102, China.

Background: Degenerative lumbar instability (DLI) is a common disease that causes low back pain (LBP) in clinic. It is difficult to completely recover from DLI, and it occurs repeatedly, which seriously affects the quality of life of patients. The epidemiological survey showed that 20-30% of low back pain was related to lumbar instability. Increasing evidence shows that seated lumbar rotation manipulation can effectively improve the clinical symptoms of patients with low back pain. The primary aim of this clinical trial is to observe the intervention effect of seated lumbar rotation manipulation on DLI patients.

Method/design: A total of 60 participants with DLI will be recruited and randomly allocated into the seated lumbar rotation manipulation group (the intervention group) or lumbar traction in supine position group (the control group) in this prospective, outcome assessor-blind, two-arm randomized controlled clinical trial. The treatment of the two groups lasted for 3 weeks, and the manipulation of the intervention group would be carried out once every other day, three times a week, a total of 9 times; the control group would be given lumbar traction once a day, five times a week, a total of 15 times. JOA (Japanese Orthopaedic Association) and VAS (Visual Analogue Scales) scores will be recorded as the primary outcomes before the treatment and at the 1st, 3rd, 5th, 8th, 10th, 12th, 15th, 17th, and 19th days after treatment and follow-up visit at the first, third, and sixth months. JOA efficacy evaluation standard will be used to evaluate the overall efficacy as the secondary outcomes.

Discussion: The results of this prospective, randomized controlled trial will provide a clinical evidence for the treatment of DLI with seated lumbar rotation manipulation.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2000032017 . Registered on 18 April 2020, Prospective registration.
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http://dx.doi.org/10.1186/s13063-021-05350-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204577PMC
June 2021

Doxorubicin-loaded natural daptomycin micelles with enhanced targeting and anti-tumor effect in vivo.

Eur J Med Chem 2021 Jun 2;222:113582. Epub 2021 Jun 2.

Ocean NanoTech, LLC, San Diego, CA, 92126, USA. Electronic address:

Development of a simple method to enhance targeting and anti-tumor effect of the chemotherapeutic agents in vivo is a major problem. Amphipathic and natural daptomycin is biocompatible antibacterial polypeptide used in clinical practice. Herein, doxorubicin (DOX) was stabilized by zwitterionic daptomycin (Dap) micelles in aqueous solution to form a zwitterionic nanodrug (Dap-DOX micelles). The hydrodynamic size and zeta potential of Dap-DOX micelles were 85 nm and -10 mV, respectively. The study on the controlled release showed that more DOX molecules were released from Dap-DOX micelles at acidic condition of tumor tissue than that at neutral condition of normal tissue which was due to pH responsiveness of Dap-DOX micelles. Dap-DOX micelles exhibited good stability in fibrinogen solution. Moreover, MTT studies showed that Dap-DOX micelles had higher cytotoxicity than free DOX. Notably, the results of flow cytometry indicated that the average fluorescence intensity of Dap-DOX micelle-treated cells was higher than that of free DOX-treated cells, and acidic conditions were more favorable for Dap-DOX micelles than normal pH in cell uptake assay. More importantly, Dap-DOX micelles were biocompatible in vivo based on the changes of weight and blood indexes of mice. Dap-DOX micelles were selectively accumulated at tumor sites in vivo through EPR effect, which reduced the toxicity of free DOX and achieved excellent tumor inhibition effect. The tumor inhibition rate of Dap-DOX micelles reached 96%. Dap-DOX micelles also effectively inhibited the growth of bacterial. Taken together, Dap-based drug delivery systems are promising and effective in cancer therapy.
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http://dx.doi.org/10.1016/j.ejmech.2021.113582DOI Listing
June 2021

Transfusion-induced platelet antibodies and regulatory T cells in multiply transfused patients.

J Clin Lab Anal 2021 Jul 14;35(7):e23864. Epub 2021 Jun 14.

Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: Platelet transfusion refractoriness (PTR) remains a difficult problem in patients requiring long-term platelet supportive care. However, there are little data on the frequency of platelet antibodies in multiply transfused Chinese patients. Moreover, the relationship between peripheral regulatory T cells (Tregs) and PTR remains unclear.

Methods: We retrospectively studied the frequency of alloimmunization against platelet antigens in patients receiving multiple transfusions between 2013 and 2017. Monoclonal antibody solid-phase platelet antibody test (MASPAT) kits were used to screen for platelet antibodies before each platelet transfusion. Peripheral Tregs and CD4 CD25 CD127 T cells were detected by flow cytometry, while transforming growth factor-beta (TGF-β) and interleukin (IL)-17 cytokines were detected by enzyme-linked immunosorbent assay.

Results: A total of 399 patients who met the inclusion criteria were enrolled for the analysis of platelet antibodies and refractoriness. Among these patients, 10 (2.5%) were positive for platelet antibodies before transfusion and 47 (11.8%) became antibody-positive during the study period. The number of alloimmunized patients was significantly higher in patients with hematological disease as compared with other disease groups (p < 0.05). Refractoriness and alloimmunization occurred in 77 (19.3%) and 22 (28.6%) patients, respectively. There were no significant differences in CD4 , CD8 , and CD4 CD25 CD127 T cell numbers and plasma levels of TGF-β1 and IL-17 between patients with PTR and the control group.

Conclusions: Refractoriness was common in patients undergoing multiple platelet transfusions (19.3%), with alloimmunization observed in 28.6% of patients. However, Tregs in peripheral blood may not play a key role in PTR.
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http://dx.doi.org/10.1002/jcla.23864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275002PMC
July 2021

Peroxidase-Like Platinum Clusters Synthesized by Polysaccharide for Sensitively Colorimetric Detection of Dopamine.

Molecules 2021 May 6;26(9). Epub 2021 May 6.

Key Laboratory of Applied Chemistry, Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.

The sensitive and selective detection of dopamine (DA) is very important for the early diagnosis of DA-related diseases. In this study, we reported the colorimetric detection of DA using polysaccharide (GLP) stabilized platinum nanoclusters (Pt-GLP NCs). When Pt-GLP NCs was added, 3,3',5,5'-tetramethylbenzidine (TMB) was rapidly catalyzed and oxidized to blue oxTMB, indicating the peroxidase-like activity of Pt-GLP NCs. The catalytic reaction on the substrate TMB followed the Michaelis-Menton kinetics with the ping-pong mechanism. The mechanism of the colorimetric reaction was mainly due to the formation of hydroxyl radical (•OH). Furthermore, the catalytic reaction of Pt-GLP NCs was used in the colorimetric detection of DA. The linear range for DA was 1-100 μM and the detection limit was 0.66 μM. The sensitive detection of DA using Pt-GLP NCs with peroxidase-like activity offers a simple and practical method that may have great potential applications in the biotechnology field.
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http://dx.doi.org/10.3390/molecules26092738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125108PMC
May 2021

Functional consequences of a rare missense BARD1 c.403G>A germline mutation identified in a triple-negative breast cancer patient.

Breast Cancer Res 2021 May 1;23(1):53. Epub 2021 May 1.

State Key Laboratory of Genetic Engineering, School of Life Sciences and Shanghai Cancer Center, Fudan University, Shanghai, China.

We identified a rare missense germline mutation in BARD1 (c.403G>A or p.Asp135Asn) as pathogenic using integrated genomics and transcriptomics profiling of germline and tumor samples from an early-onset triple-negative breast cancer patient who later was administrated with a PARP inhibitor for 2 months. We demonstrated in cell and mouse models that, compared to the wild-type, (1) c.403G>A mutant cell lines were more sensitive to irradiation, a DNA damage agent, and a PARP inhibitor; (2) c.403G>A mutation inhibited interaction between BARD1 and RAD51 (but not BRCA1); and (3) c.403G>A mutant mice were hypersensitive to ionizing radiation. Our study shed lights on the clinical interpretation of rare germline mutations of BARD1.
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http://dx.doi.org/10.1186/s13058-021-01428-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088670PMC
May 2021

Biocompatible Platinum Nanoclusters Prepared Using Bitter Gourd Polysaccharide for Colorimetric Detection of Ascorbic Acid.

Biomolecules 2021 Apr 28;11(5). Epub 2021 Apr 28.

State Key Laboratory of Metastable Materials Science and Technology, Key Laboratory of Applied Chemistry, Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse, Yanshan University, Qinhuangdao 066004, China.

Ascorbic acid is an organic compound with antioxidant properties that can protect the human body from the threat of free radicals. Therefore, it is important to detect the existence and measure the concentration of ascorbic acid to regulate its content in the human body. In this work, we prepared bitter gourd polysaccharide (BGP)-stabilized platinum nanoclusters (Pt-BGP NCs) by reacting BGP with KPtCl. Pt-BGP NCs and catalyzed the decomposition of HO to generate •OH radicals, which could oxidize TMB to form oxidized TMB (oxTMB), indicating their peroxidase-like properties. The kinetics followed the Michaelis-Menten equation. Furthermore, the colorimetric detection of ascorbic acid using Pt-BGP NCs showed high selectivity and a low detection limit of 0.191 μM. The accuracy of real sample detection using Pt-BGP NCs was as high as 98.9%. More importantly, Pt-BGP NCs had high cell biocompatibility and extremely low hemolysis rate due to the component of BGP. In summary, the prepared Pt-BGP NCs with reductive activity and good biocompatibility have good application prospects in colorimetric detection of ascorbic acid.
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http://dx.doi.org/10.3390/biom11050647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146894PMC
April 2021

A Remote Sensor System Based on TDLAS Technique for Ammonia Leakage Monitoring.

Sensors (Basel) 2021 Apr 2;21(7). Epub 2021 Apr 2.

State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun 130012, China.

The development of an efficient, portable, real-time, and high-precision ammonia (NH) remote sensor system is of great significance for environmental protection and citizens' health. We developed a NH remote sensor system based on tunable diode laser absorption spectroscopy (TDLAS) technique to measure the NH leakage. In order to eliminate the interference of water vapor on NH detection, the wavelength-locked wavelength modulation spectroscopy technique was adopted to stabilize the output wavelength of the laser at 6612.7 cm, which significantly increased the sampling frequency of the sensor system. To solve the problem in that the light intensity received by the detector keeps changing, the signal processing technique was adopted. The practical application results proved that the signal processing technique had a satisfactory suppression effect on the signal fluctuation caused by distance changing. Using Allan deviation analysis, we determined the stability and limit of detection (LoD). The system could reach a LoD of 16.6 ppm·m at an average time of 2.8 s, and a LoD of 0.5 ppm·m at an optimum averaging time of 778.4 s. Finally, the measurement result of simulated ammonia leakage verified that the ammonia remote sensor system could meet the need for ammonia leakage detection in the industrial production process.
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http://dx.doi.org/10.3390/s21072448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036584PMC
April 2021

Manipulating Berry curvature of SrRuO thin films via epitaxial strain.

Proc Natl Acad Sci U S A 2021 May;118(18)

State Key Laboratory of Low Dimensional Quantum Physics and Department of Physics, Tsinghua University, 100084 Beijing, China;

Berry curvature plays a crucial role in exotic electronic states of quantum materials, such as the intrinsic anomalous Hall effect. As Berry curvature is highly sensitive to subtle changes of electronic band structures, it can be finely tuned via external stimulus. Here, we demonstrate in SrRuO thin films that both the magnitude and sign of anomalous Hall resistivity can be effectively controlled with epitaxial strain. Our first-principles calculations reveal that epitaxial strain induces an additional crystal field splitting and changes the order of Ru orbital energies, which alters the Berry curvature and leads to the sign and magnitude change of anomalous Hall conductivity. Furthermore, we show that the rotation of the Ru magnetic moment in real space of a tensile-strained sample can result in an exotic nonmonotonic change of anomalous Hall resistivity with the sweeping of magnetic field, resembling the topological Hall effect observed in noncoplanar spin systems. These findings not only deepen our understanding of anomalous Hall effect in SrRuO systems but also provide an effective tuning knob to manipulate Berry curvature and related physical properties in a wide range of quantum materials.
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http://dx.doi.org/10.1073/pnas.2101946118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106296PMC
May 2021

Accessing Improbable Foldamer Shapes with Strained Macrocycles.

Chemistry 2021 Apr 27. Epub 2021 Apr 27.

CBMN (UMR 5248), Université de Bordeaux, CNRS, Bordeaux Institut National Polytechnique, 2 rue Robert Escarpit, 33600, Pessac, France.

The alkylation of some secondary amide functions with a dimethoxybenzyl (DMB) group in oligomers of 8-amino-2-quinolinecarboxylic acid destabilizes the otherwise favored helical conformations, and allows for cyclization to take place. A cyclic hexamer and a cyclic heptamer were produced in this manner. After DMB removal, X-ray crystallography and NMR show that the macrocycles adopt strained conformations that would be improbable in noncyclic species. The high helix folding propensity of the main chain is partly expressed in these conformations, but it remains frustrated by macrocyclization. Despite being homomeric, the macrocycles possess inequivalent monomer units. Experimental and computational studies highlight specific fluxional pathways within these structures. Extensive simulated annealing molecular dynamics allow for the prediction of the conformations for larger macrocycles with up to sixteen monomers.
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http://dx.doi.org/10.1002/chem.202101201DOI Listing
April 2021

Mechanochromic properties in a mononuclear Cu(I) complex without cuprophilic interactions.

Chem Commun (Camb) 2021 May;57(41):5082-5085

Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory of Rare Earth Materials Chemistry and Applications, Beijing Engineering Technology Research Centre of Active Display, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.

Two polymorphs of Cu[(3,4-bis(diphenylphosphino)thiophene)(bis(pyrazol-1-yl)borohydrate)] (1) were isolated. The blue luminescent crystals have evident mechanochromic luminescent (MCL) properties. Based on photophysical and structural analysis, the pore structure in the blue crystals is considered to be the main reason for the MCL properties.
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http://dx.doi.org/10.1039/d1cc01229kDOI Listing
May 2021

Transcriptomic and metabolomic insights into the variety of sperm storage in oviduct of egg layers.

Poult Sci 2021 Jun 4;100(6):101087. Epub 2021 Mar 4.

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

In birds, the sperm storage tubules (SST) are dispersed in uterovaginal junction (UVJ) and highly correlated with differential capacity of sperm storage (SS) in and among species with unspecified mechanisms. Here, the SS duration of 252 egg layer breeders was evaluated in 5 rounds with 3 phenotypic traits to screen high- and low-SS individuals, respectively, followed with transcriptome of UVJ tissues and metabolome of serum (high-SS vs. low-SS) to decipher the candidate genes and biochemical markers correlated with differential SS capacity. Histological characterization suggested slightly higher density of SST in UVJ (high-SS vs. low-SS). Transcriptome analyses identified 596 differentially expressed genes (336 upregulated vs. 260 downregulated), which were mainly enriched in gene ontology terms of homeostasis, steroid and lipid metabolism and hormone activity, and 12 significant pathways (P < 0.05) represented by calcium, steroid, and lipid metabolism. Immunohistochemical staining of GNAQ, ST6GAL1, ADFP, and PCNA showed similar distribution in UVJ tissues between 2 groups. Several candidates (HSD11B2, DIO2, AQP3, GNAQ, NANS, ST6GAL1) combined with 4 (11β-prostaglandin F2α, prostaglandin B1, 7α-hydroxytestosterone, and N-acetylneuraminic acid) of 40 differential metabolites enriched in serum metabolome were considered as regulators and biomarkers of SS duration in egg layer breeders. The integrated transcriptome and metabolome analyses of chicken breeder hens will provide novel insights for exploration and improvement of differential SS capacity in birds.
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http://dx.doi.org/10.1016/j.psj.2021.101087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082553PMC
June 2021

HLA zygosity increases risk of hepatitis B virus-associated hepatocellular carcinoma.

J Infect Dis 2021 Apr 14. Epub 2021 Apr 14.

Genomics Research Center, Academia Sinica, Taipei, Taiwan.

Background: Diversity in the human leukocyte antigen (HLA) genes might be associated with disease outcomes - the heterozygote advantage hypothesis. We tested this hypothesis in relation to hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC).

Methods: We utilized DNA from >10,000 Taiwanese individuals with current or past HBV infection to examine the association between HLA diversity and critical natural history steps in the progression from HBV infection to HCC. Individuals were classified as homozygotes at a given locus when imputed to carry the same 4-digit allele for the two HLA alleles at that locus.

Results: Increase in number of homozygous HLA class II loci was associated with an increased risk of chronic HBV infection (Ptrend=1.18×10 -7). Among chronic HBV carriers, increase in number of homozygous HLA class II loci was also associated with an increased risk of HBV-associated HCC (Ptrend=0.031). For individual HLA loci, HLA-DQB1 homozygosity was significantly associated with HCC risk (adjusted hazard ratio=1.40, 95% confidence interval=1.06-1.84). We also found that zygosity affects risk of HCC through its ability to affect viral control.

Conclusions: Homozygosity at HLA class II loci, particularly HLA-DQB1, is associated with a higher risk of HBV-associated HCC.
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http://dx.doi.org/10.1093/infdis/jiab207DOI Listing
April 2021

Treatment of polyacrylamide-polluted wastewater using a revolving algae biofilm reactor: Pollutant removal performance and microbial community characterization.

Bioresour Technol 2021 Jul 7;332:125132. Epub 2021 Apr 7.

School of Marine Science, Yantai University, Yantai 264000, China.

Industries such as oil mining face challenges in the treatment of polyacrylamide (PAM)-containing wastewater produced during petroleum extraction. The feasibility of using revolving algae biofilm (RAB) reactors to treat PAM-contaminated wastewater for simultaneous removal of carbon and nitrogen was evaluated. The presence or absence of external nitrogen sources had a significant impact on the treatment effect of the RAB system. With the additional N source, the PAM, COD, TOC, and TN removal rates were 64.1 ± 2.0, 58 ± 1.5, 34.5 ± 1.5, and 85 ± 6.0%, respectively. High-throughput sequencing showed that the biofilms on RAB reactors contained a variety of bacteria, cyanobacteria, and green algae, degrading PAM through various mechanisms. The results of infrared spectroscopy analysis indicate that the product of these processes was carboxylic acid. Based on these results, it was concluded that RAB systems can be effectively applied to the treatment of polymer-containing wastewater.
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http://dx.doi.org/10.1016/j.biortech.2021.125132DOI Listing
July 2021

Efficiency comparison of B6(Cg)-Tyr /J and C57BL/6NTac embryos as hosts for the generation of knockout mice.

Transgenic Res 2021 06 12;30(3):275-281. Epub 2021 Apr 12.

CAM-SU Genomic Resource Center, Soochow University, Suzhou, 215123, Jiang Su, China.

Careful selection of the host embryo is critical to the efficient production of knockout (KO) mice when injecting mouse embryonic stem (mES) cells into blastocysts. B6(Cg)-Tyr/J (B6 albino) and C57BL/6NTac (B6NTac) strains of mice are widely used to produce host blastocysts for such procedures. Here, we tested these two strains to identify an appropriate match for modified agouti C57BL/6N (JM8A3.N1) mES cells. When comparing blastocyst yield, super-ovulated B6NTac mice produced more injectable blastocysts per female than B6 albino mice (8.2 vs. 5.4). There was no significant difference in birth rate when injected embryos were transferred to the same pseudopregnant recipient strain. However, the live birth rate was significantly higher for B6NTac blastocysts than B6 albino blastocysts (62.7% vs. 50.2%). In addition, the proportion of pups exhibiting high-level and complete chimerism, as identified by coat color, was also significantly higher in the B6NTac strain. There was no obvious difference in the efficiency of germline transmission (GLT) when compared between B6NTac and B6 albino host embryos (61.5% vs. 63.3% for mES clones; 64.5% vs. 67.9% for genes, respectively), thus suggesting that an equivalent GLT rate could be obtained with only a few blastocyst injections for B6NTac embryos. In conclusion, our data indicate that B6NTac blastocysts are a better choice for the microinjection of JM8A3.N1 mES cells than B6 albino blastocysts.
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http://dx.doi.org/10.1007/s11248-021-00248-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169498PMC
June 2021
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