Publications by authors named "Zhiwei Huang"

266 Publications

Long-Term Increase in Cholesterol Is Associated With Better Cognitive Function: Evidence From a Longitudinal Study.

Front Aging Neurosci 2021 17;13:691423. Epub 2021 Jun 17.

Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, China.

Higher visit-to-visit cholesterol has been associated with cognitive decline. However, the association between long-term increase or decrease in cholesterol and cognitive decline remains unclear. A total of 4,915 participants aged ≥45 years with normal cognition in baseline were included. The participants were divided into four groups, namely low-low, low-high, high-low, and high-high, according to the diagnostic thresholds of total cholesterol (TC), non-high-density lipoprotein cholesterol (NHDL-C), low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol (HDL-C) after 4 years of follow-up. Cognitive function was assessed by episodic memory and mental intactness. Binary logistic regression was used to analyse the association of cholesterol variation with cognitive decline. Among the participants, 979 (19.9%) experienced global cognitive decline. The odds ratio (OR) of global cognitive and memory function decline were remarkably lower in participants in the low-high NHDL-C group than those in the low-low group [OR and 95% confidence interval (CI): 0.50 [0.26-0.95] for global cognitive decline, 0.45 [0.25-0.82] for memory function decline]. The lower OR was also significant in females (OR [95% CI]: 0.38 [0.17-0.87] for global cognitive decline; 0.44 [0.19-0.97] for memory function decline) and participants without cardiovascular disease (OR [95% CI]: 0.31 [0.11-0.87] for global cognitive decline; 0.34 [0.14-0.83] for memory function decline). The increases in other cholesterol were also negatively associated with the risk of cognitive decline although not significantly. A longitudinal increase in NHDL-C may be protective for cognition in females or individuals without cardiovascular disease.
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http://dx.doi.org/10.3389/fnagi.2021.691423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248815PMC
June 2021

Clinical applications of mesenchymal stromal cell-based therapies for pulmonary diseases: An Update and Concise Review.

Int J Med Sci 2021 1;18(13):2849-2870. Epub 2021 Jun 1.

Department of Clinical Laboratory Medicine, the Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, China.

Lung disorders are a leading cause of morbidity and death worldwide. For many disease conditions, no effective and curative treatment options are available. Mesenchymal stromal cell (MSC)-based therapy is one of the cutting-edge topics in medical research today. It offers a novel and promising therapeutic option for various acute and chronic lung diseases due to its potent and broad-ranging immunomodulatory activities, bacterial clearance, tissue regeneration, and proangiogenic and antifibrotic properties, which rely on both cell-to-cell contact and paracrine mechanisms. This review covers the sources and therapeutic potential of MSCs. In particular, a total of 110 MSC-based clinical applications, either completed clinical trials with safety and early efficacy results reported or ongoing worldwide clinical trials of pulmonary diseases, are systematically summarized following preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines, including acute/viral pulmonary disease, community-acquired pneumonia (CAP), chronic obstructive pulmonary disease (COPD), bronchopulmonary dysplasia (BPD), interstitial lung diseases (ILD), chronic pulmonary fibrosis, bronchiolitis obliterans syndrome (BOS) and lung cancer. The results of recent clinical studies suggest that MSCs are a promising therapeutic approach for the treatment of lung diseases. Nevertheless, large-scale clinical trials and evaluation of long-term effects are necessary in further studies.
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http://dx.doi.org/10.7150/ijms.59218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241779PMC
June 2021

Multi-particle interaction in AC electric field driven by dielectrophoresis force.

Electrophoresis 2021 Jun 12. Epub 2021 Jun 12.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, P. R. China.

When the dielectrophoresis technology is used to manipulate micron-sized particles, the interaction between particles should not be ignored because of the particle-particle interaction. Especially, when multiple particles (number of particles is above 2) are simultaneously manipulated, the interaction between neighboring particles will affect the results of the manipulation. This research investigates the interaction of particles caused dielectrophoresis effect by the Arbitrary Lagrangian-Eulerian (ALE) method based on the hypothesis of the thin layer of the electric double layer at the microscale. The mathematics model can be solved simultaneously by the finite element method for the AC electric field, the flow field around the suspended particles and the particle mechanics at the micrometer scale. In this study, the particle conductivity and the direction of the electric field are investigated, we find that particle conductivity and electric field direction pose an impact on particle movement, and the research reveal the law of microparticle dielectrophoresis movement, which could offer theoretical and technology support to profoundly understand the precise manipulation of particles in microfluidic chips by the dielectrophoresis effect.
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http://dx.doi.org/10.1002/elps.202100094DOI Listing
June 2021

Structural basis of Staphylococcus aureus Cas9 inhibition by AcrIIA14.

Nucleic Acids Res 2021 06;49(11):6587-6595

Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology, 150080 Harbin, China.

Bacteriophages have evolved a range of anti-CRISPR proteins (Acrs) to escape the adaptive immune system of prokaryotes, therefore Acrs can be used as switches to regulate gene editing. Herein, we report the crystal structure of a quaternary complex of AcrIIA14 bound SauCas9-sgRNA-dsDNA at 2.22 Å resolution, revealing the molecular basis for AcrIIA14 recognition and inhibition. Our structural and biochemical data analysis suggest that AcrIIA14 binds to a non-conserved region of SauCas9 HNH domain that is distinctly different from AcrIIC1 and AcrIIC3, with no significant effect on sgRNA or dsDNA binding. Further, our structural data shows that the allostery of the HNH domain close to the substrate DNA is sterically prevented by AcrIIA14 binding. In addition, the binding of AcrIIA14 triggers the conformational allostery of the HNH domain and the L1 linker within the SauCas9, driving them to make new interactions with the target-guide heteroduplex, enhancing the inhibitory ability of AcrIIA14. Our research both expands the current understanding of anti-CRISPRs and provides additional culues for the rational use of the CRISPR-Cas system in genome editing and gene regulation.
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http://dx.doi.org/10.1093/nar/gkab487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216286PMC
June 2021

MoO/TiO catalyst with atomically dispersed O-Mo-O structures toward improving NHHSO poisoning resistance for selective catalytic reduction of nitrogen oxides.

J Hazard Mater 2021 Jun 2;418:126289. Epub 2021 Jun 2.

Department of Environmental Science & Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, Fujian 361021, PR China. Electronic address:

Slow progress in discovering new catalysts to circumvent the problem of ammonium bisulfate (NHHSO, ABS) poisoning has hindered further development of selective catalytic reduction (SCR) technology of NO with ammonia (from numerous industrial processes) in afterburning systems at temperatures below dew point of ABS (typically between 280 °C and 320 °C). Recently, we have explored the use of atomically dispersed Mo species on TiO particles (hereafter denoted as MoO/TiO) as highly efficient catalyst for NH-SCR reaction. In the present study, it will be shown that this type of catalyst is highly resistant to ABS poisoning for NH-SCR reaction, overcoming a major issue afflicting the application of commercial VO-WO/TiO catalyst at temperatures below the dew point of ABS. Aberration-corrected scanning transmission electron microscopy (STEM) suggests that most of the Mo species are present in atomically dispersed form in the MoO/TiO catalyst. SO oxidation measurements show that the MoO/TiO catalyst exhibits a substantially lower SO oxidation rate compared to the commercial VO-WO/TiO, mitigating ABS formation. Furthermore, decomposition of ABS on MoO/TiO surface is found to be extremely facile. Temperature-programmed surface reaction (TPSR) with NO shows that the decomposition temperature of ABS over MoO/TiO is 70 °C lower than that found on the commercial VO-WO/TiO catalyst. Our investigations provide valuable information for the development of NH-SCR catalysts with exceptional resistance to ABS poisoning for NO emission control.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126289DOI Listing
June 2021

Prognostic Value of Baseline d-Dimer Level in Patients With Coronary Artery Disease: A Meta-Analysis.

Angiology 2021 Jun 3:33197211019805. Epub 2021 Jun 3.

Coronary Heart Disease Center, Fuwai Hospital, National Center for Cardiovascular Diseases, 34736Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

The prognostic significance of d-dimer level in patients with coronary artery disease (CAD) is not fully established. This meta-analysis aimed to examine the association between elevated d-dimer level at baseline and adverse outcomes in patients with CAD. Two independent authors comprehensively searched PubMed and Embase databases from their inception to December 31, 2020. All observational studies reporting the values of baseline d-dimer level in predicting the major adverse cardiovascular events (MACEs) or survival outcomes in patients with CAD were included. The prognostic values were calculated by pooling adjusted RR with 95% CI for the highest versus the lowest d-dimer level. Thirteen studies consisting of 25 600 patients with CAD were identified. Comparison between the highest and lowest d-dimer level showed that the pooled multivariable adjusted RR was 1.69 (95% CI, 1.53-1.86) for all-cause mortality, 2.37 (95% CI, 1.52-3.69) for cardiovascular mortality, and 1.44 (95% CI, 1.19-1.74) for MACEs, respectively. Elevated blood level of d-dimer at baseline was independently associated with higher risk of MACEs, cardiovascular death, and all-cause mortality in patients with CAD. The baseline d-dimer level may have important prognostic value in patients with CAD.
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http://dx.doi.org/10.1177/00033197211019805DOI Listing
June 2021

The Divergent Key Residues of Two () CheY Paralogs Play a Key Role in Distinguishing Their Functions.

Microorganisms 2021 May 24;9(6). Epub 2021 May 24.

College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.

The chemotactic response regulator CheY, when phosphorylated by the phosphoryl group from phosphorylated CheA, can bind to the motor switch complex to control the flagellar motor rotation. (previous name: ), a phytopathogen, carries two paralogous genes, and . The functional difference of two paralogous CheYs remains unclear. Three -deletion mutants were constructed to test the effects of two CheYs on the chemotaxis of . Phenotypes of three -deletion mutants show that deletion of each significantly affects the chemotactic response, but -deletion possesses more prominent effects on the chemotactic migration and swimming pattern of than does -deletion. CheA-dependent cellular localization of two CheY paralogs and in vitro pull-down of two CheY paralogs by FliM demonstrate that the distinct roles of two CheY paralogs arise mainly from the differentiation of their binding affinities for the motor switch component FliM, agreeing with the divergence of the key residues on the motor-binding surface involved in the interaction with FliM. The single respective replacements of key residues R93 and A109 on the motor-binding surface of CheY2 by alanine (A) and valine (V), the corresponding residues of CheY1, significantly enhanced the function of CheY2 in regulating the chemotactic response of CheY-deficient mutant Δy to nutrient substances and host attractants. These results conclude that the divergence of the key residues in the functional subdomain is the decisive factor of functional differentiation of these two CheY homologs and protein function may be improved by the substitution of the divergent key residues in the functional domain for the corresponding residues of its paralogs. This finding will help us to better understand how paralogous proteins sub-functionalize. In addition, the acquirement of two CheY2 variants, whose chemotactic response functions are significantly improved, will be very useful for us to further explore the mechanism of CheY to bind and regulate the flagellar motor and the role of chemotaxis in the pathogenicity of .
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http://dx.doi.org/10.3390/microorganisms9061134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225110PMC
May 2021

Metagenomic analysis reveals oropharyngeal microbiota alterations in patients with COVID-19.

Signal Transduct Target Ther 2021 05 13;6(1):191. Epub 2021 May 13.

School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.

COVID-19 remains a serious emerging global health problem, and little is known about the role of oropharynx commensal microbes in infection susceptibility and severity. Here, we present the oropharyngeal microbiota characteristics identified by shotgun metagenomic sequencing analyses of oropharynx swab specimens from 31 COVID-19 patients, 29 influenza B patients, and 28 healthy controls. Our results revealed a distinct oropharyngeal microbiota composition in the COVID-19 patients, characterized by enrichment of opportunistic pathogens such as Veillonella and Megasphaera and depletion of Pseudopropionibacterium, Rothia, and Streptococcus. Based on the relative abundance of the oropharyngeal microbiome, we built a microbial classifier to distinguish COVID-19 patients from flu patients and healthy controls with an AUC of 0.889, in which Veillonella was identified as the most prominent biomarker for COVID-19 group. Several members of the genus Veillonella, especially Veillonella parvula which was highly enriched in the oropharynx of our COVID-19 patients, were also overrepresented in the BALF of COVID-19 patients, indicating that the oral cavity acts as a natural reservoir for pathogens to induce co-infections in the lungs of COVID-19 patients. We also found the increased ratios of Klebsiella sp., Acinetobacter sp., and Serratia sp. were correlated with both disease severity and elevated systemic inflammation markers (neutrophil-lymphocyte ratio, NLR), suggesting that these oropharynx microbiota alterations may impact COVID-19 severity by influencing the inflammatory response. Moreover, the oropharyngeal microbiome of COVID-19 patients exhibited a significant enrichment in amino acid metabolism and xenobiotic biodegradation and metabolism. In addition, all 26 drug classes of antimicrobial resistance genes were detected in the COVID-19 group, and were significantly enriched in critical cases. In conclusion, we found that oropharyngeal microbiota alterations and functional differences were associated with COVID-19 severity.
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http://dx.doi.org/10.1038/s41392-021-00614-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116522PMC
May 2021

Understanding the Benefits of Standing and Sitting Baduanjin Based on Cardiopulmonary Exercise Testing: An Observational Study.

J Altern Complement Med 2021 May 6. Epub 2021 May 6.

The Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, China.

Although Baduanjin (a traditional Chinese physical activity) has been reported to promote general health, the optimal exercise intensity and kinematic characteristics of this intervention remain poorly understood. This investigation aimed to quantify and compare the exercise intensities of traditional standing Baduanjin (TB) and sitting Baduanjin (SB) using cardiopulmonary exercise testing, to further clarify the sources of the previously observed benefits of this modality. Observational study. Healthy individuals were recruited to perform TB, SB, and cycling (in order) until they reached their ventilatory threshold. Intensity-relevant parameters based on type of exercise and specific time points (exercise start and the end of the 2nd, 4th, 6th, and 8th set of motion) were compared between TB and SB with ventilatory threshold as control. Forty individuals (18 male and 22 female) completed the trial. Significant differences in peak oxygen uptake, metabolic equivalent of task, and Borg scale existed among the three exercise types, indicating a decreasing overall exercise intensity in the order of ventilatory threshold, TB, and SB. All parameters except the respiratory exchange ratio fluctuated significantly across the time points. Both TB and SB resulted in a significantly lower exercise intensity when compared with the ventilatory threshold established through cycling exercise. The benefits of Baduanjin might be explained partly by its appropriate exercise intensity and intermittent intensity pattern. Baduanjin might be a potential alternative to existing schemes for exercise rehabilitation.
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http://dx.doi.org/10.1089/acm.2020.0458DOI Listing
May 2021

Perspectives on anti-IL-1 inhibitors as potential therapeutic interventions for severe COVID-19.

Cytokine 2021 07 17;143:155544. Epub 2021 Apr 17.

The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin 300211, China. Electronic address:

The overproduction of proinflammatory cytokines, resulting in what has been described as a cytokine storm or cytokine release syndrome (CRS), may be the key factor in the pathology of severe coronavirus disease 2019 (COVID-19) and is also a crucial cause of death from COVID-19. With the purpose of finding effective and low-toxicity drugs to mitigate CRS, IL-1 blockade agents, which are one of the safest ways to stop this overwhelming innate immune response, are already available in several preliminary reports or are under observational trials and may offer an important treatment option in hyperinflammatory COVID-19. In this review, we described the key information in both case reports and clinical studies on the potential beneficial features of IL-1 inhibitors in COVID-19 patients.
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http://dx.doi.org/10.1016/j.cyto.2021.155544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052468PMC
July 2021

Rationalizing the promotional effect of Mn oxides in benzene combustion using an O 2p-band center descriptor.

Chem Commun (Camb) 2021 May;57(40):4942-4945

Department of Environmental Science & Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, Fujian 361021, China.

Our work sheds light on using the O 2p-band center as a useful electronic descriptor for understanding the variations in catalytic reducibility of transition metal oxides (TMOs) and the promotional effect of MnO2 during catalytic benzene combustion. The "volcano"-type activity plot, in conjunction with the reduction characteristic of the TMOs, ultimately reflects the Sabatier principle, which states that a good catalyst (i.e., MnO2) balances the capability of oxygen abstraction and uptake in the case of benzene combustion.
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http://dx.doi.org/10.1039/d1cc00912eDOI Listing
May 2021

Correction to: Adaptive immune responses to SARS-CoV-2 infection in severe versus mild individuals.

Signal Transduct Target Ther 2021 Apr 19;6(1):161. Epub 2021 Apr 19.

HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150080, China.

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http://dx.doi.org/10.1038/s41392-021-00540-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054129PMC
April 2021

The predictive value of systemic immune inflammation index for postoperative survival of gallbladder carcinoma patients.

J Surg Oncol 2021 Jul 25;124(1):59-66. Epub 2021 Mar 25.

Department of General Surgery (Hepatobiliary Surgery), The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.

Background: Growing evidence indicates that systemic immune inflammation index (SII) can predict the prognosis of various solid tumors. The objective of this study aimed to investigate the efficacy of SII in predicting the prognosis of gallbladder carcinoma (GBC) patients after radical surgery.

Methods: A consecutive series of 93 patients with GBC who underwent radical resection were enrolled in the retrospective study. The cutoff value for the SII was calculated using the time-dependent receiver operating characteristic (ROC) curve analysis by overall survival (OS) prediction. The associations between the SII and the clinicopathologic characteristics were analyzed using Pearson's χ test and Fisher's exact test. Survival curves were calculated using the Kaplan-Meier method. Univariate analysis was performed to evaluate the prognostic relevance of preoperative parameters. The multivariate Cox regression proportional hazard model was used to assess variables significant on univariate analysis.

Results: The Kaplan-Meier survival analysis and the multivariate analysis of patients with GBC who received radical resection showed SII independently predicted OS. The univariate analysis showed that the TNM stage, SII, CA19-9, ALP, prealbumin, NLR, MLR, lymph node metastasis, and histopathological type were all associated with overall survival. In time-dependent ROC analysis, the area of the SII-CA19-9 under the ROC curve (AUC) was higher than that of the preoperative SII or CA19-9 levels for the prediction of OS.

Conclusion: Our results demonstrate that high SII was a predictor of poor long-term outcomes among patients with GBC undergoing curative surgery. SII-CA19-9 classification may be more effective in predicting the postoperative prognosis of GBC patients.
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http://dx.doi.org/10.1002/jso.26470DOI Listing
July 2021

Association between dietary diversity and cognitive impairment among the oldest-old: Findings from a nationwide cohort study.

Clin Nutr 2021 Apr 2;40(4):1452-1462. Epub 2021 Mar 2.

Department of Epidemiology, School of Public Health (Guangdong Provincial Key Laboratory of Tropical Disease Research), Southern Medical University, Guangzhou, Guangdong, China. Electronic address:

Background & Aims: Dietary diversity is widely recommended in national and international guidelines; however, whether the beneficial effects on cognitive function still apply in the oldest-old (80+) has rarely been studied. This study aimed to evaluate the associations of dietary diversity with cognitive function among the oldest-old in a large prospective cohort in China.

Methods: We conducted a long-term prospective analysis on 11,970 participants aged 80+ (6581 octogenarians, 3730 nonagenarians, and 1659 centenarians). We constructed the baseline dietary diversity score (DDS) based on eight food items of a food frequency questionnaire. Mini-mental state examination (MMSE) was used to classify the participants as having cognitive impairment or not and was also used as a continuous metric. Non-linear associations of DDS with cognitive impairment was evaluated by cox models with penalized splines. We used mixed-effect models for longitudinal data with repeated measurements of MMSE (for up to seven time during the follow-up between 1998 and 2014).

Results: We documented 4778 cognitive impairment during 46,738 person-years of follow-up. Each one unit increase in DDS was associated with a 4% lower risk of cognitive impairment (adjusted hazard ratio (HR): 0.96; 95% confidential interval (CI): 0.94-0.98). Compared to participants with DDS of 0 score, those with a DDS of 1-2, 3-4, and higher than 5 scores had a lower cognitive impairment risk, the HRs were 0.86 (0.79-0.95), 0.82 (0.74-0.91), and 0.72 (0.64-0.82) respectively, and a significant trend emerged (p < 0.001). Compared with DDS of zero score, a DDS of 1-2,3-4, ≥5 was related to slower MMSE decline (β = 0.128, 0.162, 0.301, respectively, p < 0.01).

Conclusions: Even after the age of 80, dietary diversity may offer a simple and straightforward mean of identifying and screening individuals at high risk for cognitive impairment. Recommendation of dietary diversity may be advocated to attenuate cognitive decline and decrease the risk of cognitive impairment in the oldest-old, especially in a low income or middle-income countries.
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http://dx.doi.org/10.1016/j.clnu.2021.02.041DOI Listing
April 2021

COVID-19 immune features revealed by a large-scale single-cell transcriptome atlas.

Cell 2021 04 3;184(7):1895-1913.e19. Epub 2021 Feb 3.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, China.

A dysfunctional immune response in coronavirus disease 2019 (COVID-19) patients is a recurrent theme impacting symptoms and mortality, yet a detailed understanding of pertinent immune cells is not complete. We applied single-cell RNA sequencing to 284 samples from 196 COVID-19 patients and controls and created a comprehensive immune landscape with 1.46 million cells. The large dataset enabled us to identify that different peripheral immune subtype changes are associated with distinct clinical features, including age, sex, severity, and disease stages of COVID-19. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA was found in diverse epithelial and immune cell types, accompanied by dramatic transcriptomic changes within virus-positive cells. Systemic upregulation of S100A8/A9, mainly by megakaryocytes and monocytes in the peripheral blood, may contribute to the cytokine storms frequently observed in severe patients. Our data provide a rich resource for understanding the pathogenesis of and developing effective therapeutic strategies for COVID-19.
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http://dx.doi.org/10.1016/j.cell.2021.01.053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857060PMC
April 2021

Role of IL-6 inhibitor in treatment of COVID-19-related cytokine release syndrome.

Int J Med Sci 2021 21;18(6):1356-1362. Epub 2021 Jan 21.

Department of Clinical Laboratory Medicine, the Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, China.

Cytokine release syndrome (CRS) may be the key factor in the pathology of severe coronavirus disease 2019 (COVID-19). As a major driver in triggering CRS in patients with COVID-19, interleukin-6 (IL-6) appears to be a promising target for therapeutics. The results of inhibiting both trans- and classical- signaling with marketed IL-6 inhibitors (tocilizumab, siltuximab and sarilumab) in severe COVID-19 patients are effective based on several small studies and case reports thus far. In this review, we described the evidence of the IL-6 response in patients with COVID-19, clarified the pathogenesis of the role of IL-6-mediated CRS in severe COVID-19, and highlighted the rationale for the use of anti-IL-6 agents and key information regarding the potential features of these IL-6 inhibitors in COVID-19 patients.
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http://dx.doi.org/10.7150/ijms.53564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893562PMC
April 2021

Contrasting Thermoelectric Transport Behaviors of -Type PbS Caused by Doping Alkali Metals (Li and Na).

Research (Wash D C) 2020 3;2020:4084532. Epub 2020 Dec 3.

School of Materials Science and Engineering, Beihang University, Beijing 100191, China.

PbS is a latent substitute of PbTe thermoelectric materials, which is on account of its superiority in low cost and earth abundance. Here, the thermoelectric transport properties of -type PbS by doping alkali metals (Na and Li) are investigated and it is verified that Li is a more effective dopant than Na. By introducing Li, the electrical and thermal transport properties were optimized collectively. The electrical transport properties were boosted remarkably via adjusting carrier concentration, and the maximum power factor (PF) of ~11.5 W/cmK and average power factor (PF) ~9.9 W/cmK between 423 and 730 K in PbLiS were achieved, which are much higher than those (~9.5 and ~7.7 W/cmK) of PbNaS. Doping Li and Na can weaken the lattice thermal conductivity effectively. Combining the enlarged PF with suppressed total thermal conductivity, a maximum ZT ~0.5 at 730 K and a large average ZT ~0.4 at 423-730 K were obtained in -type PbLiS, which are higher than ~0.4 and ~0.3 in -type PbNaS, respectively.
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http://dx.doi.org/10.34133/2020/4084532DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877382PMC
December 2020

Stable single atomic silver wires assembling into a circuitry-connectable nanoarray.

Nat Commun 2021 Feb 19;12(1):1191. Epub 2021 Feb 19.

Department of Environmental Science and Engineering, Fudan University, Shanghai, China.

Atomic metal wires have great promise for practical applications in devices due to their unique electronic properties. Unfortunately, such atomic wires are extremely unstable. Here we fabricate stable atomic silver wires (ASWs) with appreciably unoccupied states inside the parallel tunnels of α-MnO nanorods. These unoccupied Ag 4d orbitals strengthen the Ag-Ag bonds, greatly enhancing the stability of ASWs while the presence of delocalized 5s electrons makes the ASWs conducting. These stable ASWs form a coherently oriented three-dimensional wire array of over 10 nm in width and up to 1 μm in length allowing us to connect it to nano-electrodes. Current-voltage characteristics of ASWs show a temperature-dependent insulator-to-metal transition, suggesting that the atomic wires could be used as thermal electrical devices.
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http://dx.doi.org/10.1038/s41467-021-21462-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895918PMC
February 2021

Author Correction: Structural basis of assembly of the human T cell receptor-CD3 complex.

Nature 2021 Feb;590(7846):E52

HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.

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http://dx.doi.org/10.1038/s41586-021-03245-4DOI Listing
February 2021

Clioquinol inhibits cell growth in a SERCA2-dependent manner.

J Biochem Mol Toxicol 2021 May 28;35(5):e22727. Epub 2021 Jan 28.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.

Clioquinol has been reported to act as a potential therapy for neurodegenerative diseases and cancer. However, the underlying mechanism is unclear. We have previously reported that clioquinol induces S-phase cell cycle arrest through the elevation of calcium levels in human neurotypic SH-SY5Y cells. In this study, different types of cells were observed to detect if the effect of clioquinol on intracellular calcium levels is cell type-specific. The Cell Counting Kit-8 assay showed that clioquinol exhibited varying degrees of concentration-dependent cytotoxicity in different cell lines, and that the growth inhibition caused by it was not related to cell source or carcinogenesis. In addition, the inhibition of cell growth by clioquinol was positively associated with its effect on intracellular calcium content ([Ca ] ). Furthermore, the elevation of [Ca ] induced by clioquinol led to S-phase cell cycle arrest. Similar to our previous studies, the increase in [Ca ] was attributed to changes in the expression levels of the calcium pump SERCA2. Comparison of expression levels of SERCA2 between cell lines showed that cells with high levels of SERCA2 were more sensitive to clioquinol. In addition, analysis using UALCAN and the Human Protein Atlas also showed that the expression of SERCA2 in the corresponding human tissues was similar to that of the cells tested in this study, suggesting potential in the application of clioquinol in the future. In summary, our results expand the understanding of the molecular mechanism of clioquinol and provide an important strategy for the rational use of clioquinol.
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http://dx.doi.org/10.1002/jbt.22727DOI Listing
May 2021

Mapping the Intratumoral Heterogeneity in Glioblastomas with Hyperspectral Stimulated Raman Scattering Microscopy.

Anal Chem 2021 02 14;93(4):2377-2384. Epub 2021 Jan 14.

Optical Bioimaging Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, 117576, Singapore.

Recent genomic studies on the glioblastoma (GBM) subtypes (, mesenchymal, proneural, and classical) pave a way for effective clinical treatments of the recurrent brain tumor. However, identification of the GBM subtype is complicated by the intratumoral heterogeneity that results in coexistence of multiple subtypes within the tissue specimen. Here, we present the use of hyperspectral stimulated Raman scattering (SRS) microscopy for rapid, label-free molecular assessment of GBM intratumoral heterogeneity with submicron resolution. We develop a unique label-free Raman imaging diagnostic platform consisting of the spectral focusing hyperspectral SRS imaging of the large-area GBM tissue specimens, SRS images, and spectrum retrieval using the multivariate curve resolution algorithm and subtype classification based on the quadratic support vector machine model for rapid molecular subtyping of GBMs. Both the stain-free SRS histological images and 2D subtype maps can be obtained within 20-30 min which is superior to the days of the conventional single-cell RNA sequencing. While the SRS histology assesses the demyelination status as a new diagnostic feature, the SRS mapping provides a new insight into intratumoral heterogeneity across GBM tissue specimens. We find that the major proportions of the GBM tissues agree with the diagnostic results of the genomic analysis, but nontrivial portions of the remaining SRS image tiles in the specimens are found to belong to other molecular subtypes, implying the substantial degree of GBM heterogeneity. The rapid SRS imaging diagnostic platform developed has shown the ability of unveiling tumor heterogeneity in GBM tissues accurately, which would promote the improvement of the GBM-targeted therapy in near future.
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http://dx.doi.org/10.1021/acs.analchem.0c04262DOI Listing
February 2021

Label-Free Follow-Up Surveying of Post-Treatment Efficacy and Recurrence in Nasopharyngeal Carcinoma Patients with Fiberoptic Raman Endoscopy.

Anal Chem 2021 02 6;93(4):2053-2061. Epub 2021 Jan 6.

Optical Bioimaging Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576.

Recurrent nasopharyngeal carcinoma (NPC) is the main cause of poor prognosis for NPC patients after chemo- and radiotherapies. Subsequent long-term follow-ups of post-treatment patients are crucial for the early discovery of tumor recurrence with timely intervention. Current clinical imaging methods based on tissue morphology encounter difficulties in differentiating recurrent tumors from post-treatment inflammation and fibrosis. In this work, we apply a unique fiberoptic Raman endoscopy technique to address the challenges for label-free follow-up surveying of post-treatment NPC patients and accurate detection of tumor recurrence. Significant Raman spectral differences can be observed among normal, NPC, and nonrecurring post-treatment patients. Raman endoscopy provides diagnostic accuracy of 100% for detecting recurrent NPC from early post-treatment inflammation and diagnostic accuracy of 98.21% for separating recurrent NPC from long-term post-treatment fibrosis. Further quantitative Raman modeling on nasopharyngeal tissue Raman data acquired unveils the changes of major tissue biochemicals (e.g., triolein, elastin, keratin, fibrillar collagen, and type IV collagen) associated with primary NPC and post-treatment recurrent NPC tissue compared to normal nasopharyngeal tissue. This work demonstrates that fiberoptic Raman endoscopy can be a clinically powerful diagnostic tool for rapid, label-free post-treatment surveying and recurrent tumor detection in NPC patients at the molecular level.
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http://dx.doi.org/10.1021/acs.analchem.0c03778DOI Listing
February 2021

Experimental and theoretical investigation of the enhancement of the photo-oxidation of Hg by CeO-modified morphology-controlled anatase TiO.

J Hazard Mater 2021 Mar 2;406:124535. Epub 2020 Dec 2.

Institute of Environmental Engineering, National Sun Yat-Sen University, No. 70, Lian-Hai Road, Kaohsiung 804, Taiwan. Electronic address:

This study aims to investigate the coeffects of predominantly exposed anatase TiO{001} and {101} and CeO loading on the photo-oxidation of Hg to relieve the adverse effects caused by higher temperatures of 50-250 °C. The effect of loading CeO on the photocatalytic activity of morphology-controlled TiO was not only investigated using DFT with U correction but also experimentally analyzed by characterizing the electrochemical properties and the formation of free radicals. The theoretical calculation showed that CeO loading on TiO{101} was more stable than that on TiO{001}. Accordingly, a larger portion of CeO was observed to anchor to the (101) plane than to the (001) plane. CeO loading is more beneficial for increasing the distribution of photo-induced electrons and holes on the surface of 7%CeTi than on the surface of TiO and increases the energy difference between the conduction band edge of 7%CeTi and the standard redox potential of O/·O. Correspondingly, the photocatalytic removal efficiencies (PREs) of Hg by 7%CeTi were significantly enhanced compared with those of pristine TiO. The effect of CeO was highly morphologically dependent on the photocatalytic activity. This study provides valuable insight into surface engineering strategies for morphology-controlled photocatalysts for air pollution control technology.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124535DOI Listing
March 2021

Spontaneous internal hemorrhage of a giant hepatic hemangioma with infection: a case report and literature review.

J Int Med Res 2020 Dec;48(12):300060520976474

Department of Hepatobiliary Surgery, The Affiliated Hospital of Southwest Medical University, Sichuan, China.

Spontaneous internal hemorrhage from a hepatic hemangioma is rare. This case describes a 59-year-old woman who was hospitalized with recurrent cough and fever for 6 months. The doctor thought that she had pneumonia, but other infectious diseases could not be ruled out. Therefore, related tests were performed and strong antibiotics were used, but the symptom of fever was persistent and recurred. Enhanced computed tomography (CT) findings showed a right hepatic giant hemangioma with hemorrhage, while tuberculosis, liver abscess, and immune disease were excluded by the physician. Because the patient's fever was associated with a large hepatic hemangioma, the patient was transferred to surgery. During treatment, the amount of bleeding increased, so she underwent a right hepatic hemangioma resection in the emergency department. Her postoperative fever symptoms subsequently resolved. Pathological examination confirmed hemorrhagic necrosis with infection in hepatic hemangioma. Follow-up showed that the patient was afebrile.
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http://dx.doi.org/10.1177/0300060520976474DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724420PMC
December 2020

Silver Single-Atom Catalyst for Efficient Electrochemical CO Reduction Synthesized from Thermal Transformation and Surface Reconstruction.

Angew Chem Int Ed Engl 2021 Mar 29;60(11):6170-6176. Epub 2021 Jan 29.

Department of Chemistry, Tsinghua University, Beijing, 100084, P. R. China.

We report an Ag single-atom catalyst (Ag /MnO ), which was synthesized from thermal transformation of Ag nanoparticles (NPs) and surface reconstruction of MnO . The evolution process of Ag NPs to single atoms is firstly revealed by various techniques, including in situ ETEM, in situ XRD and DFT calculations. The temperature-induced surface reconstruction process from the MnO (211) to (310) lattice plane is critical to firmly confine the existing surface of Ag single atoms; that is, the thermal treatment and surface reconstruction of MnO is the driving force for the formation of single Ag atoms. The as-obtained Ag /MnO achieved 95.7 % Faradic efficiency at -0.85 V vs. RHE, and coupled with long-term stability for electrochemical CO reduction reaction (CO RR). DFT calculations indicated single Ag sites possessed high electronic density close to Fermi Level and could act exclusively as the active sites in the CO RR. As a result, the Ag /MnO catalyst demonstrated remarkable performance for the CO RR, far surpassing the conventional Ag nanosized catalyst (Ag /MnO ) and other reported Ag-based catalysts.
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http://dx.doi.org/10.1002/anie.202014718DOI Listing
March 2021

The value of multiparametric histogram features based on intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) for the differential diagnosis of liver lesions.

Ann Transl Med 2020 Sep;8(18):1128

Department of Radiology, Guangzhou Panyu Center Hospital, Guangzhou, China.

Background: The present study analyzed whole-lesion histogram parameters from intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) to explore the clinical value of IVIM histogram features in the differentiation of liver lesions.

Methods: In this retrospective study, 33 cases of hepatic hemangioma (HH), 22 cases of hepatic cysts (HC), and 34 cases of hepatocellular carcinoma (HCC) were underwent IVIM-DWI (b =0-600 s/mm), which were confirmed pathologically and clinically. The data were processed by IVIM model to obtain the following quantitative indicators: perfusion fraction (f), slow diffusion coefficient (D), and pseudo-diffusion coefficient (or fast diffusion coefficient, D*). The region of interest in the largest solid part of the lesion was delineated for histogram analysis of the correlation between tissue image and lesion type. The relevant histogram parameters were obtained and statistically analyzed. The characteristic histogram parameters for HH, HC, and HCC were compared to find significantly different parameters. The diagnostic efficacies of these parameters for HH, liver cysts, and HCC were assessed using the receiver operating characteristic (ROC) curves.

Results: There were significant differences in the maximum diameter, maximum value, minimum value, mean, median, standard deviation, uniformity, skewness, kurtosis, volume, 10th percentile (P10) of D, and 90th percentile (P90) of D between the three groups (P<0.05). The maximum diameter, minimum value, entropy, and volume of D* differed significantly between the three groups (P<0.05). The maximum diameter, minimum value, mean, median, skewness, kurtosis, volume, P10, and P90 of f differed significantly between the three groups (P<0.05). The largest area under the ROC curve (AUC) for both D* and f was that of volume (AUC =0.883 for both). When 1438.802 was used as the volume cut-off, the sensitivity and specificity of volume in differentiating between HH and HC were 87.88 and 77.27, respectively, and the sensitivity and specificity of volume in differentiating between HC and HCC were 77.27 and 85.29.

Conclusions: A multiparametric histogram from IVIM-DWI magnetic resonance imaging (MRI) is an effective means of identifying HH, HC, and HCC that provides valuable reference information for clinical diagnosis.
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http://dx.doi.org/10.21037/atm-20-5109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576072PMC
September 2020

Revascularization Versus Medical Therapy in Takayasu's Arteritis Patients with Coronary Artery Involvement.

Rheumatol Ther 2021 Mar 23;8(1):119-133. Epub 2020 Nov 23.

Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China.

Introduction: Treatment strategies to improve clinical outcomes in Takayasu's arteritis (TA) with coronary lesions have ranged from pharmacological therapy to invasive procedures, such as coronary angioplasty, stenting, and surgery. However, the therapeutic strategy for this kind of patient is still unclear. This study aimed to investigate the clinical characteristics and influence of revascularization versus medical therapy in TA patients with coronary artery involvement.

Methods: We analyzed the medical records of 806 TA patients between January 2008 and December 2019. Clinical features and treatment were analyzed, and patients were categorized into medical treatment and revascularization. Additionally, patients were sorted as percutaneous coronary intervention (PCI) and coronary artery bypass surgery (CABG) based on the strategy of revascularization.

Results: Ninety cases with coronary lesions induced by TA were enrolled. Among 90 cases, 39 patients adopted conservative treatment, and 51 patients received revascularization (28 subjects with PCI, 23 participants with CABG). The median follow-up time was 63 (45-91) months. There is no significant difference in cardiovascular death between medical treatment and revascularization (2/39, 5.1% vs. 5/51, 9.8%, P = 0.971). The analysis of subgroup indicated that the mortality caused by cardiovascular disease was also similar in the CABG and PCI (2/28, 7.1% vs. 3/23, 13.0%, P = 0.772). However, the proportion of restenosis is much higher in the PCI compared with that of CABG (39.3%, 8.7%, P = 0.022, respectively). Heart failure is an independent predictor of death in these patients.

Conclusions: There is no significant difference in cardiovascular death between medical treatment and revascularization. The analysis of the subgroup indicated that the mortality caused by cardiovascular disease was also similar in the CABG and PCI, but the restenosis is much higher in the PCI compared with that of CABG. Heart failure is an independent predictor of death in these patients.
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http://dx.doi.org/10.1007/s40744-020-00251-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991006PMC
March 2021

Construction of Hybrid Bimetallic Uranyl Compounds Based on a Preassembled Terpyridine Metalloligand.

Chemistry 2021 Jan 30;27(6):2124-2130. Epub 2020 Dec 30.

Laboratory of Nuclear Energy Chemistry, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, P. R. China.

Six hybrid uranyl-transition metal compounds [UO Ni(cptpy) (HCOO) (DMF)(H O)] (1), [UO Ni(cptpy) (BTPA) ] (2), [UO Fe(cptpy) (HCOO) (DMF)(H O)] (3), [UO Fe(cptpy) (BTPA) ] (4), [UO Co(cptpy) (HCOO) (DMF)(H O)] (5), and [UO Co(cptpy) (BTPA) ] (6), based on bifunctional ligand 4'-(4-carboxyphenyl)-2,2':6',2''-terpyridine (Hcptpy) are reported (H BTPA = 4,4'-biphenyldicarboxylic acid). Single-crystal XRD revealed that all six compounds feature similar metalloligands, which consist of two cptpy anions and one transition metal cation. The metalloligand M(cptpy) can be considered to be an extended linear dicarboxylic ligand with length of 22.12 Å. Compounds 1, 3, and 5 are isomers, and all of them feature 1D chain structures. The adjacent 1D chains are connected together by hydrogen bonds and π-π interactions to form a 3D porous structure, which is filled with solvent molecules and can be exchanged with I . Compounds 2, 4, and 6 are also isomers, and all of them feature 2D honeycomb (6,3) networks with hexagonal units of dimensions 41.91×26.89 Å, which are the largest among uranyl compounds with honeycomb networks. The large aperture allows two sets of equivalent networks to be entangled together to result in a 2D+2D→3D polycatenated framework. Remarkably, these uranyl compounds exhibit high catalytic activity for cycloaddition of carbon dioxide. Moreover, the geometric and electronic structures of compounds 1 and 2 are systematically discussed on the basis of DFT calculations.
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http://dx.doi.org/10.1002/chem.202004344DOI Listing
January 2021

Polysaccharides from the Fleshy Fruits of Attenuate the Kidney Injury in High-Fat Diet/Streptozotocin-Induced Diabetic Mice.

J Med Food 2020 Dec 22;23(12):1275-1286. Epub 2020 Oct 22.

School of Basic Medicine, and Gannan Medical University, Ganzhou, China.

The fleshy fruits of Abel are the immature fruits of , which are infected by Massee and then turn to be intumescent and hollowed. They contain rich trace elements and vitamin C and are eaten directly as wild fruits in the Chinese countryside. Recent studies report that has anti-inflammatory and antioxidative effects. The current study, for the first time, evaluates the renal protective capacity of polysaccharides from the fleshy fruits of (CFFP) in streptozotocin-induced diabetic mice fed high-fat diets. The diabetic mice were orally administered CFFP for 3 months to evaluate the renoprotective function of CFFP. Our results indicated that 250 mg/kg CFFP significantly alleviated diabetes-induced renal injury by decreasing serum creatine, blood urea nitrogen levels, the kidney/body weight ratio, expression of fibronectin and collagen, as well as the secretion of tumor necrosis factor- and interleukin-6. Additionally, 250 mg/kg CFFP could significantly ameliorate renal oxidative stress through increasing glutathione levels and lowering malondialdehyde contents. We confirmed that CFFP could exert antioxidative, anti-inflammatory, and antifibrosis activities. CFFP might be a potential therapeutic agent, and the fleshy fruits of might be a diet therapy for diabetic patients in the future.
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http://dx.doi.org/10.1089/jmf.2020.4717DOI Listing
December 2020

Comment on "Preoperative anxiety induces chronic postoperative pain by activating astrocytes in the anterior cingulate cortex region".

Rev Assoc Med Bras (1992) 2020 09;66(9):1308

Department of Endocrinology, the First College of 'YYClinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang, Hubei 443003, China.

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http://dx.doi.org/10.1590/1806-9282.66.9.1308DOI Listing
September 2020
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