Publications by authors named "Zhiqiang Zhu"

160 Publications

Down-Regulation of Associated With Abnormal Ciliogenesis During Embryonic Neurodevelopment Under Inositol Deficiency.

Front Neurol 2021 19;12:579998. Epub 2021 May 19.

Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, China.

The inositol polyphosphate-5-phosphatase E () gene is located on chromosome 9q34.3. The enzyme it encodes mainly hydrolyzes the 5-phosphate groups of phosphatidylinositol (3,4,5)-trisphosphate (PtdIns (3,4,5) P3) and phosphatidylinositol (4,5)-bisphosphate (PtdIns (4,5)P2), which are closely related to ciliogenesis and embryonic neurodevelopment, through mechanisms that are largely unknown. Here we studied the role of gene in ciliogenesis during embryonic neurodevelopment using inositol-deficiency neural tube defects (NTDs) mouse and cell models. Confocal microscopy and scanning electron microscope were used to examine the number and the length of primary cilia. The dynamic changes of expression in embryonic murine brain tissues were observed during Embryonic Day 10.5-13.5 (E 10.5-13.5). Immunohistochemistry, western blot, polymerase chain reaction (PCR) arrays were applied to detect the expression of and cilia-related genes of the embryonic brain tissues in inositol deficiency NTDs mouse. Real-time quantitative PCR (RT-qPCR) was used to validate the candidate genes in cell models. The levels of inositol and PtdIns(3,4) P2 were measured using gas chromatography-mass spectrometry (GC-MS) and enzyme linked immunosorbent assay (ELISA), respectively. Our results showed that the expression levels of gradually decreased in the forebrain tissues of the control embryos, but no stable trend was observed in the inositol deficiency NTDs embryos. expression in inositol deficiency NTDs embryos was significantly decreased compared with the control tissues. The expression levels of gene and the PtdIns (3,4) P2 levels were also significantly decreased in the inositol deficient cell model. A reduced number and length of primary cilia were observed in NIH3T3 cells when inositol deficient. Three important cilia-related genes () were down-regulated significantly in the inositol-deficient NTDs mouse and cell models, and was highly involved in NTDs. In summary, these findings suggested that down-regulation of might be associated with abnormal ciliogenesis during embryonic neurodevelopment, under conditions of inositol deficiency.
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http://dx.doi.org/10.3389/fneur.2021.579998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170399PMC
May 2021

Continuous flooding stimulates root iron plaque formation and reduces chromium accumulation in rice (Oryza sativa L.).

Sci Total Environ 2021 Sep 17;788:147786. Epub 2021 May 17.

Key Laboratory of Information Traceability for Agricultural Products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

Chromium (Cr) contamination in rice poses a serious threat to human health. Therefore, we conducted pot experiments to investigate the influence of water management regimes on the formation of iron plaque on rice roots, and its effect on the accumulation and translocation of Cr in rice grown on contaminated soil. The results showed that water management regimes, including continuous and intermittent flooding, exerted notable effects on soil solution concentrations of Cr(VI) and Cr(III) through changes in redox potential, pH, and dissolved Fe(II) concentrations. In particular, 69.2%-71.8% of Cr(VI) was reduced to Cr(III) under continuous flooding, whereas only 33.3%-38.6% was reduced under intermittent flooding conditions. Additionally, continuous flooding created a rhizosphere environment favorable to the formation of iron plaque. The amount of iron plaque formed increased by 28.2%-47.2% under continuous flooding conditions as compared with that under intermittent flooding conditions. Moreover, compared with intermittent flooding, under continuous flooding, more Cr (18.0%-23.9%) was adsorbed in the iron plaque, thereby sequestering Cr and reducing its mobility. The Cr concentrations in rice root, straw, husk, and grain under continuous flooding conditions were, respectively, 32.0%-36.5%, 32.7%-36.3%, 34.2%-46.9%, and 25.4%-37.7% lower than those under intermittent flooding conditions. Therefore, continuous flooding caused a substantial decrease in the Cr concentrations in rice tissues, as well as an increased distribution of Cr in the iron plaque that acted as a barrier to reduce Cr transfer to the rice roots. These results indicate that continuous flooding irrigation was effective in minimizing the accumulation of Cr in rice plants, as it not only enhanced Cr(VI) reduction in the soil but also improved the blocking capacity of the iron plaque.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147786DOI Listing
September 2021

Evolutionary Analysis of Pre-S/S Mutations in HBeAg-Negative Chronic Hepatitis B With HBsAg < 100 IU/ml.

Front Public Health 2021 26;9:633792. Epub 2021 Apr 26.

Clinical Medical Examination Center, Northern Jiangsu People's Hospital, Yangzhou, China.

Hepatitis B surface antigen (HBsAg) and viral load are important clinical indicators for antiviral therapy. Few studies have evaluated viral sequence biomarkers predicting the risk of hepatocellular carcinoma (HCC) in the stage, which show a low serological response (HBsAg < 100 IU/ml) and high viral levels (HBV DNA > 2,000 IU/ml). This study aims to determine the trend of the biological prevalence within the pre-S/S regions of special model of inactive CHB infection. We used Sanger sequencing, quantitative HBV serology (HBeAg and HBsAg), and liver function index to identify whether HBV genome sequences are associated with long-term risk of further HCC progression in special inactive CHB infection. HBV sequencing analysis of 28 CHB patients with special infectious pattern showed higher genetic diversity among four opening reading frames (ORFs) ( < 0.001). However, dN/dS ratios of HBsAg and pre-C/C regions in the experimental group showed no significantly different from those in the HCC group ( = 0.06), while significantly lower in polymerase and HBxAg regions of the experimental group ( < 0.001). In addition, seven positively selected sites were identified in pre-S1, five in pre-S2, and four in S, in which five sites (128H/135Q/135R/139L/141P) were among "α" determinant. These mutations in the pre-S/S region might be associated with the HCC phenotype of low HBsAg expression, with the P region possibly impacting high viral loads. Increased viral diversity across the HBV genome is also associated with low levels of HBsAg. The cumulative evolutionary changes in the HBV pre-S/S regions shows that facilitate immune evasion should be monitored individually. Due to the similarity of evolutionary characteristics in HCC, low serological responses and high viremia may be associated with the risk of further disease progression.
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http://dx.doi.org/10.3389/fpubh.2021.633792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107265PMC
May 2021

Association between medical male circumcision and HIV risk compensation among heterosexual men: a systematic review and meta-analysis.

Lancet Glob Health 2021 Jul 30;9(7):e932-e941. Epub 2021 Apr 30.

School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China; Kirby Institute, University of New South Wales, Sydney, NSW, Australia; School of Public Health, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Background: Medical male circumcision (MMC) reduces HIV infection among heterosexual men. There are concerns MMC might prompt higher-risk sexual behaviours because of lower self-perceived risk of HIV infection. We reviewed the published literature to examine associations between MMC and both condom use and number of sex partners among heterosexual men.

Methods: In this systematic review and meta-analysis, we searched PubMed, Embase, and the Cochrane Library for studies published before Nov 15, 2020. Interventional and observational studies were included if they contained original quantitative data describing the association between MMC and condom use or number of sex partners among heterosexual men. We excluded data from men whose circumcisions were ritual or religious and data from men who have sex with men. We extracted odds ratios (ORs) and 95% CIs for the associations between MMC and condomless sex and MMC and multiple sex partners directly from the publications if available, selecting adjusted ORs when provided; when necessary, we calculated ORs and 95% CIs using original study data provided in the publication. We used the Mantel-Haenszel random effects model to calculate pooled ORs and 95% CIs.

Findings: Our search yielded 3035 results, of which 471 were duplicates and 2537 did not meet the inclusion criteria. From the remaining 27 eligible studies, we identified 99 292 men from 31 independent population samples. 24 studies were done in Africa. We found no statistically significant associations between MMC and condomless sex (OR 0·91, 95% CI 0·80-1·05; k=30; I=88·7%) or multiple sex partners (1·02, 0·88-1·18; k=27; I=90·1%). No associations between MMC and condomless sex or multiple sexual partners were found in any subgroup analyses by study design, income of country, age, recruitment setting, circumcision assessment, circumcision prevalence, and risk of publication bias.

Interpretation: The promotion of circumcision as an HIV preventive measure does not appear to increase higher-risk sexual behaviours in heterosexual men. Ongoing sexual health education should be maintained as a vital component of effective MMC programmes.

Funding: National Science and Technology Major Project of China, the Fundamental Research Funds for the Central Universities, and the Shenzhen Science and Technology Innovation Commission Basic Research Program.
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http://dx.doi.org/10.1016/S2214-109X(21)00102-9DOI Listing
July 2021

Co-Cropping Indian Mustard and Silage Maize for Phytoremediation of a Cadmium-Contaminated Acid Paddy Soil Amended with Peat.

Toxics 2021 Apr 21;9(5). Epub 2021 Apr 21.

The Institute of Agriculture, UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA 6009, Australia.

Co-cropping is an eco-friendly strategy to improve the phytoremediation capacity of plants growing in soils contaminated with heavy metals such as cadmium (Cd). This study was conducted to investigate the effects of co-cropping Indian mustard () and silage maize () and applying peat on the phytoremediation of a Cd-contaminated acid paddy soil via characterizing plant growth and Cd uptake in pot experiments. There were six planting patterns (Control: no plants; MI-2 and MI-4: mono-cropping of Indian mustard at low and high densities, respectively; MS: mono-cropping of silage maize; CIS-2 and CIS-4: co-cropping of Indian mustard at low and high densities with silage maize, respectively) and two application rates of peat (NP: 0; WP: 30 g kg). When Indian mustard and silage maize were co-cropped, the shoot biomass of Indian mustard plants per pot was significantly ( < 0.05) lower than that obtained in the mono-cropping systems, with a substantial reduction (55-72%) in the same plant density group. The shoot biomass of silage maize plants in the mono-cropping systems did not differ significantly from that in the co-cropping systems regardless of the density of Indian mustard. The growth-promoting effect of the peat application was more pronounced in Indian mustard than silage maize. Under the low density of Indian mustard, the co-cropping systems significantly ( < 0.05) decreased Cd uptake by silage maize. Additionally, soil amendment with peat significantly ( < 0.05) increased shoot Cd removal rate and Cd translocation factor value in the co-cropping systems. Taken together, the results demonstrated that silage maize should be co-cropped with Indian mustard at an appropriate density in Cd-polluted soils to achieve simultaneous remediation of Cd-contaminated soils (via Indian mustard) and production of crops (here, silage maize). Peat application was shown to promote the removal of Cd from soil and translocation of Cd into shoots and could contribute to enhanced phytoremediation of Cd-contaminated acid paddy soil.
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http://dx.doi.org/10.3390/toxics9050091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143100PMC
April 2021

Human immunodeficiency virus-related renal cell carcinoma: a retrospective study of 19 cases.

Infect Agent Cancer 2021 Apr 21;16(1):26. Epub 2021 Apr 21.

Department of Urology, Beijing Youan Hospital, Capital Medical University, Beijing, China.

Purpose: We aimed to investigate basic information, clinical findings, treatments for tumor, pathology, and outcomes of HIV-positive patients diagnosed with renal cell carcinoma (RCC).

Patients And Methods: We collected 19 patients from 2012 to 2020 who are diagnosed with RCC with HIV-positive. A retrospective analysis was performed on their hospitalization course and tumor-related parameters, including basic information, clinical findings, HIV-associated data, pathology, treatments for tumor, and outcomes.

Results: In our study, patients were diagnosed with RCC at the median age of 51. Males took a great part (17 males, 89%) in all patients, while only 2 females were diagnosed. The median CD4 T lymphocyte cell count was 462 cells/μl when diagnosed with RCC (range from 111 cells/μl to 1536 cells/μl). Eleven patients diagnosed with RCC and HIV infection at the same time, who may have high viral load and low CD4 T lymphocyte cell count. Eight patients accepted a median HAART for 30 months (range from 11 months to 108 months) prior to diagnosis of RCC. All the patients performed operations successfully, and 4 of them performed partial nephrecotomy. Only 1 patient was identified with chromophobe cell carcinoma, 1 with partially clear cell and partially papillary carcinoma, and 17 with clear cell carcinoma. Two of the patients with Fuhrman grades 2-3 accepted cytokine therapy with IL-2 and IFN-α. Two patients died of lung metastasis 1 year and 6 months after surgery respectively, even though 1 patient accepted full dose targeted therapy (sorafenib) for 3 months, and one refused adjuvant therapy. The remaining 17 patients are still alive at a median follow-up of 34 months; however, 1 patient lives with lung and brain metastases at the last follow-up of 3 years after surgery.

Conclusions: RCC patients with HIV-positive were similar to the general population in terms of clinical characters, treatment measures, and pathology. RCC patients with HIV-positive seemed like to obey the same clinical practice guideline as in the general population. The outcomes of HIV-positive patients with partial nephrectomy are not inferior to patients with radical nephrectomy. Furthermore, experience in targeted therapy and immunal therapy (PD-1/PD-L1 inhibitors) needs to be learned.
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http://dx.doi.org/10.1186/s13027-021-00362-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059295PMC
April 2021

Generation of Nonspherical Liquid Metal Microparticles with Tunable Shapes Exhibiting an Electrostatic-Responsive Performance.

ACS Appl Mater Interfaces 2021 Apr 5;13(14):16677-16687. Epub 2021 Apr 5.

Department of Modern Mechanics, University of Science and Technology of China, Hefei, Anhui 230026, China.

Nonspherical liquid metal microparticles (NLMs) show extraordinary potential in various applications due to their multifunctional and structural advantages. To one-step-produce shaped NLMs with high efficiency, high controllability, and free of template, a facile microfluidic strategy named rotary flow shearing (RFS) is reported. A high-speed viscous shearing flow is provided by two counter-rotating rotors in the carrier fluid, inducing continuous pinch-off of liquid metal flowing from a capillary tube positioned in face of the slit between two rotors. The real-time oxidation realizes the rapid solidification of the pinching neck and the liquid metal surface during the RFS process, resulting in massive NLMs. Different from other microfluidic methods, the RFS enables tunable shapes of NLMs, especially for working materials at high viscosities. The collected NLMs exhibit special electrostatic-responsive performances including translation, rotation, reciprocation, and lining up under the manipulation of an external electric field. Such NLMs can be promisingly used for the construction of novel micromotors and soft electronics.
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http://dx.doi.org/10.1021/acsami.1c01026DOI Listing
April 2021

State-of-the-art and prospects of Zn-containing layered double hydroxides (Zn-LDH)-based materials for photocatalytic water remediation.

Chemosphere 2021 Sep 25;278:130367. Epub 2021 Mar 25.

School of Environmental Science and Engineering, Shaanxi University of Science and Technology, Xian, 710021, China.

With the rapid worldwide development of industry and human activities, increasing amounts of multifarious contaminants have significantly threatened environmental ecosystems and human health. Solar photocatalytic decontamination, as an environmentally friendly technology, has been regarded as a good approach to eliminate water pollutants. To date, various photocatalysts have been developed for the purpose of water remediation. Zn-containing layered double hydroxides (Zn-LDHs) and their derivatives are promising candidates due to their suitable band edge positions (oxidation-reduction potentials) for high photocatalytic performances, flexible properties derived from adjustable components and tailorable electronic structures, chemical stabilities, and low toxicities. This review focuses on the fabrication and modification of Zn-LDHs and their photocatalytic applications for the elimination of contaminants in water, including the degradation of toxic organic pollutants, transfer of hazardous heavy metals to lower toxicity heavy metals, and bacterial inactivation. The mechanisms involved in the photocatalytic processes are also thoroughly reviewed. Finally, the emerging scientific and engineering opportunities and challenges in environmental photocatalysis are presented. This review provides basic insights into the construction of Zn-LDH-based materials with high photocatalytic activities and new perspectives on their applications for the photocatalytic elimination of contaminants, which is helpful for the development of photocatalysis for environmental remediation from the lab to industry.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130367DOI Listing
September 2021

Quercetin Nanoparticle Ameliorates Lipopolysaccharide-Triggered Renal Inflammatory Impairment by Regulation of Sirt1/NF-KB Pathway.

J Biomed Nanotechnol 2021 Feb;17(2):230-241

Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, PR China.

As a conventional complication of sepsis, acute kidney injury (AKI) is characterized by high incidence and mortality. Effective management methods are still lacking. Quercetin belongs to a kind of flavonoids that exerts many functions, for example anti-inflammation and anti-fibrosis. However, its function in sepsis AKI is uncertain. Our study therefore set out to assess the function of quercetin in AKI mice model induced by lipopolysaccharide (LPS) and human proximal tubular cells (HK-2), including the potential mechanisms. Quercetin was loaded onto a biodegradable polymer carrier (nanoparticle) to enhance its bioavailability. The data showed that quercetin administration strikingly improved renal dysfunction and ameliorated tubular injury caused by LPS in mice. In mice model and in cultured cells, quercetin pretreatment obviously restrained LPS-triggered cell apoptosis and inflammation, including generation of various cytokines. Moreover, the results from mice model and cell model showed that quercetin could diminish IB and p65 phosphorylation after LPS treatment. The most significant observation of this study was that quercetin elevated the expression of Sirt1. Transfection of Sirt1 specific shRNA mitigated the suppression of quercetin on cell apoptosis, inflammation and of NF-B activation triggered by LPS. Therefore, these sequels indicate that quercetin protects against sepsis-associated AKI by upregulation Sirt1 expression through quenching NF-B activation and may be an encouraging therapeutic agent for patients with sepsis-associated AKI.
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http://dx.doi.org/10.1166/jbn.2021.3031DOI Listing
February 2021

Genetic Polymorphisms in DNA Repair Gene APE1/Ref-1 and the Risk of Neural Tube Defects in a High-Risk Area of China.

Reprod Sci 2021 Mar 24. Epub 2021 Mar 24.

Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, 100020, China.

Apurinic/apyrimidinic endonuclease 1/redox-factor 1 (APE1/Ref-1) gene encodes a multifunctional protein involved in the DNA base excision repair (BER) pathway, which initiates repair of apurinic/apyrimidinic (AP) sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone. APE1/Ref-1 polymorphisms are related to the occurrence of neural tube defects (NTDs), but the association between APE1/Ref-1 polymorphisms and NTDs is not reported in Chinese Han population. The aim of the present study was to evaluate the association of APE1/Ref-1 polymorphism and the risk of NTD occurrence for Han population in a high-risk area of China. APE1/Ref-1 genotypes were determined by iPLEX Gold SNP genotyping. AP sites and folate level of brain tissues were measured. The results showed that three polymorphisms (rs3136817, rs77794916, and rs1760944) of APE1/Ref-1 were statistically associated with NTD subtypes. Allele C of rs3136817, allele T of rs77794916, and allele G of rs1760944 were associated with an increased risk for encephalocele (OR = 2.52, 95% CI [1.25-5.07], P < 0.01; OR = 1.80, 95% CI [1.04-3.12], P = 0.04; and OR = 1.96, 95% CI [1.12-3.45], P = 0.02), compared with those harboring the alleles T, C, and T, respectively. The folate level in NTDs was lower than that in controls. DNA AP sites in the encephalocele were significantly higher than the control (P < 0.01). The three polymorphisms of APE1/Ref-1 were significantly related to NTD occurrence, which indicated that APE1/Ref-1 might be a potential genetic risk factor for encephalocele in a high-risk area of NTDs in China.
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http://dx.doi.org/10.1007/s43032-021-00537-yDOI Listing
March 2021

Deep Cycling for High-Capacity Li-Ion Batteries.

Adv Mater 2021 Mar 2;33(10):e2004998. Epub 2021 Feb 2.

Innovative Center for Flexible Devices (iFLEX), School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.

As the practical capacity of conventional Li-ion batteries (LIBs) approaches the theoretical limit, which is determined by the rocking-chair cycling architecture, a new cycling architecture with higher capacity is highly demanded for future development and electronic applications. Here, a deep-cycling architecture intrinsically with a higher theoretical capacity limit than conventional rocking-chair cycling architecture is developed, by introducing a follow-up cycling process to contribute more capacity. The deep-cycling architecture makes full use of movable ions in both of the electrolyte and electrodes for energy storage, rather than in either the electrolyte or the electrodes. Taking LiMn O -mesocarbon microbeads (MCMB)/Li cells as a proof-of-concept, 57.7% more capacity is obtained. Moreover, the capacity retention is as high as 84.4% after 2000 charging/discharging cycles. The deep-cycling architecture offers opportunities to break the theoretical capacity limit of conventional LIBs and makes high demands for new-type of cathode materials, which will promote the development of next-generation energy storage devices.
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http://dx.doi.org/10.1002/adma.202004998DOI Listing
March 2021

Comparative study on a kilowatt-MPT-MS-based method with two ion polarity modes for the inert palladium metal.

Analyst 2021 Mar;146(5):1760-1771

School of Chemistry and Environmental Science, Shangrao Normal University, Jiangxi 334001, China.

Inert metals are of much importance and play a key role in modern industrial manufacturing. The analytical techniques of inert metals remain challenging. In particular, the mass spectrometry of inert metal elements is yet to be further developed, which also limits the contemporary conceptual in situ analysis of inert metals. As the representative element, the mass spectral detection of palladium is critical and of far-reaching significance. Herein, we developed a mass spectrometry method, which can be used for the high-speed and in situ analysis of palladium, and even for other inert metals. Combining the line ion trap mass spectrometer with the versatile ambient ionization source, a novel kilowatt microwave plasma torch (MPT) can be used to obtain the fully characteristic MPT mass spectra of palladium. Detailed multistage tandem mass spectra show that the general form of target ions is [M(O2)x(NO)mNy(NO2)n]- for the negative ion mode and [M(H2O)x(NO2)y(N2)m]+ for the positive ion mode. Moreover, the formation and evolution of these palladium complex ions were reasonably derived based on the analysis of MPT background mass spectra. This mass spectrometric technique is also suitable for the determination of the palladium-containing solution in the sub-trace level. Semi-quantitative results showed that the detecting ability for palladium in the negative mode is better than that of the positive mode. Under the negative ion mode, the limit of detection (LOD) for m/z 259 were evaluated to be 0.5 μg L-1 under the optimized conditions of the negative mode, with the linear range of 1-100 μg·L-1 (R2 ≥ 0.9985) and the relative standard deviation (RSD, n = 11) being in the range of 1.20%-5.98% (refer to Table S3). Our experimental data showed that MPT-MS was a promising technique for providing another alternative in the on-site analysis of liquid samples and other intimate relevant fields, as the supplement of ICP-MS for the detection of inert metal elements. On the other hand, this work will also certainly promote the more broad applications of platinum-group elements (PGE) in modern science and industry.
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http://dx.doi.org/10.1039/d0an02071kDOI Listing
March 2021

LncRNA MAFG-AS1 Promotes the Progression of Bladder Cancer by Targeting the miR-143-3p/COX-2 Axis.

Pathobiology 2020 25;87(6):345-355. Epub 2020 Nov 25.

Department of Urology, The Third Central Hospital of Tianjin, Tianjin, China.

Background: Long noncoding RNAs (lncRNAs) are potential biomarkers that are very important for the development of cancer. Studies show that lncRNAs are significantly correlated with the carcinogenesis and progression of bladder cancer (BLCA). In this research, we aimed at probing into the role of lncRNA MAFG-AS1 in the tumorigenesis of BLCA.

Methods: RT-qPCR was employed to detect MAFG-AS1 expression in BLCA tissues and cells. MAFG-AS1 siRNA and overexpression plasmid were transfected into 5637 and T24 BLCA cell lines to inhibit or upregulate MAFG-AS1 expression, respectively, and then the regulatory functions of MAFG-AS1 on BLCA cell proliferation, migration, and invasion were assessed using cell counting kit-8 (CCK-8) assay, EdU method, and Transwell experiments, respectively. Dual-luciferase reporter assay and RNA immunoprecipitation were conducted to validate the targeting relationships between MAFG-AS1 and miR-143-3p, and miR-143-3p and COX-2. In addition, miR-143-3p was repressed in MAFG-AS1-silenced 5637 and T24 cell lines, and the function of MAFG-AS1/miR-143-3p axis in BLCA cells was further evaluated. The regulatory effects of MAFG-AS1 and miR-143-3p on the expression of COX-2 protein were detected by Western blot.

Results: MAFG-AS1 was remarkably upregulated in BLCA patient tissues and cell lines, and its high expression was closely related to histological grade, tumor size, and lymph node metastasis. Silencing of MAFG-AS1 inhibited BLCA cell proliferation, metastasis, and invasion, while overexpression of MAFG-AS1 in BLCA cells had opposite biological effects. MAFG-AS1 was proved to target miR-143-3p to repress its expression. Moreover, it was confirmed that MAFG-AS1 and miR-143-3p could modulate COX-2 expression.

Conclusion: The MAFG-AS1/miR-143-3p/COX-2 axis contributes to BLCA progression.
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http://dx.doi.org/10.1159/000509957DOI Listing
November 2020

Blind Estimation Methods for BPSK Signal Based on Duffing Oscillator.

Sensors (Basel) 2020 Nov 10;20(22). Epub 2020 Nov 10.

Science and Technology on Electromechanical Dynamic Control Laboratory, School of Mechatronical Engineering, Beijing Institute of Technology, Beijing 100081, China.

To realize the blind estimation of binary phase shift keying (BPSK) signal, this paper describe a new relational expression among the state of Duffing oscillator excited by BPSK signal, the pseudo-random code of BPSK signal, and the difference frequency between the to-be-detect signal and internal drive force signal of Duffing oscillator. Two output characteristics of Duffing oscillators excited by BPSK signals named implied periodicity and pilot frequency array synchronization are presented according to the different chaotic states of Duffing oscillator. Then two blind estimation methods for the carrier frequency and pseudo-random sequence of the BPSK signal are proposed based on these two characteristics, respectively. These methods are shown to have a significant effect on the parameter estimation of BPSK signals with no prior knowledge, even at very low signal-to-noise ratios (SNRs).
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http://dx.doi.org/10.3390/s20226412DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697423PMC
November 2020

C2 Alcohol Oxidation Boosted by Trimetallic PtPbBi Hexagonal Nanoplates.

ACS Appl Mater Interfaces 2020 Nov 10;12(47):52731-52740. Epub 2020 Nov 10.

Shanghai Key Laboratory of Materials Protection and Advanced Materials in Electric Power, College of Environmental and Chemical Engineering Shanghai University of Electric Power, Yangpu District, 2588 Changyang Road, Shanghai 200090, China.

The exploration of ternary Pt-based catalysts represents a new trend for the application of electrocatalysts in fuel cells. In the present study, intermetallic PtPbBi hexagonal nanoplates (HNPs) with a hexagonal close-packed structure have been successfully synthesized via a facile solvothermal synthesis approach. The optimized PtPbBi HNPs exhibited excellent mass activity in the ethanol oxidation reaction (8870 mA mg) in an alkaline ethanol solution, which is 12.7 times higher than that of JM Pt/C. Meanwhile, the mass activity of PtPbBi HNPs in an ethylene glycol solution (10,225 mA mg) is 1.85 times higher than that of JM Pt/C. In particular, its catalytic activity is better than that of most reported Pt-based catalysts. In addition, the optimized PtPbBi HNPs also show a better operational durability than commercial Pt/C. For the ethylene glycol oxidation reaction, a mass activity of 42.7% was retained even after a chronoamperometric test for 3600 s, which is rare among the reported Pt-based catalysts. By combining X-ray photoelectron spectroscopy and electrochemical characterization, we reveal the electron transfer between Pt, Pb, and Bi; this would lead to weakened CO adsorption and enhanced OH adsorption, thereby promoting the removal of toxic intermediates and ensuring that PtPbBi HNP samples have high activity and excellent stability. This work can inspire the design and synthesis of Pt-based nanocatalysts.
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http://dx.doi.org/10.1021/acsami.0c16215DOI Listing
November 2020

MiR-367 alleviates inflammatory injury of microglia by promoting M2 polarization via targeting CEBPA.

In Vitro Cell Dev Biol Anim 2020 Dec 4;56(10):878-887. Epub 2020 Nov 4.

Department of Emergency Intensive Care Unit, the First Affiliated Hospital of Zhengzhou University, No.1, East Jianshe Road, Erqi District, Zhengzhou, 450052, Henan Province, China.

MiR-367 was reported to regulate inflammatory response of microglia. CCAAT/enhancer-binding protein α (C/EBPA) could mediate microglia polarization. In this study, we explored the possible roles of miR-367 and CEBPA in intracerebral hemorrhage (ICH). ICH and normal specimens were obtained from the tissue adjacent to and distant from hematoma of ICH patients, respectively. Microglia were isolated and identified by immunofluorescence. The isolated microglia were treated with erythrocyte lysate and randomly divided into 8 groups using different transfection reagents. The transfection efficiency of miR-367 was determined by qRT-PCR. The expressions of M1 and M2 microglia markers were detected by Western blotting. The relationship between CEBPA and miR-367 was confirmed by dual luciferase reporter system. Flow cytometry was performed to determine the level of apoptosis in the cells transfected with miR-367 and CEBPA in erythrocyte lysate-treated microglia. We found that miR-367 expression level was downregulated in ICH specimens. Erythrocyte lysate-treated microglia was successfully established using erythrocyte lysate, as decreased miR-367 expression was observed. Overexpression of miR-367 could significantly decrease the expressions of MHC-ІІ, IL-1β, and Bax, reduced apoptosis rate, and increased the expressions of CD206, Bal-2, and Arg-1 in erythrocyte lysate-treated microglia. CEBPA was proved to be a direct target for miR-367, which could inhibit microglia M2 polarization and increase apoptosis rate. However, in the presence of both CEBPA and miR-367 mimic, the protein and mRNA expressions of CEBPA were decreased, leading to promoted microglia M2 polarization and a decreased apoptosis rate. MiR-367 regulates microglia polarization by targeting CEBPA and is expected to alleviate ICH-induced inflammatory injury.
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http://dx.doi.org/10.1007/s11626-020-00519-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723938PMC
December 2020

Synthesis of Mannich-type derivatives from amides activated by hydrogen bonding with ZnCl.

Org Biomol Chem 2020 Nov 4;18(44):9095-9099. Epub 2020 Nov 4.

Jiangxi Province Key Laboratory of Synthetic Chemistry, East China University of Technology, Nanchang 330013, Jiangxi, China. and School of Chemistry, Biology and Material Science, East China University of Technology, Nanchang 330013, Jiangxi, China.

The amide group has one of the most significant functionalities found in many natural products. Herein, low-nucleophilic amides are used in a Mannich-type reaction to synthesize N-acyl-protected amine derivatives. A highly efficient synthetic method utilizing simple aldehydes, N-substituted anilines, and amides as substrates was established through a one-pot amide pathway activated by hydrogen bonding between the ZnCl and amide under solvent-free conditions. This strategy can be broadly applied to medicinal chemistry. More importantly, compared with the previous Lewis acid catalyzed reaction, we proposed a new application of zinc chloride.
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http://dx.doi.org/10.1039/d0ob01989eDOI Listing
November 2020

Repeat hepatic resection VS radiofrequency ablation for the treatment of recurrent hepatocellular carcinoma: an updated meta-analysis.

Minim Invasive Ther Allied Technol 2020 Nov 4:1-10. Epub 2020 Nov 4.

Department of Gastrointestinal Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.

Background: This paper evaluates the efficacy and safety of repeat hepatic resection and radiofrequency ablation in the treatment of recurrent hepatocellular carcinoma.

Material And Methods: We retrieved and collected all relevant articles from the inception to 8 March 2020. After data extraction, we conducted meta-analysis and carried out the heterogeneity test, sensitivity analysis, and publication bias test to evaluate reliability.

Results: A total of 12 studies with 1746 patients (rHR 837, RFA 909) were included. rHR was similar to RFA in a one-year overall survival rate (OS), while rHR was superior to RFA in 3- and 5-year OS and 1-, 3-, and 5-year disease-free survival rates (DFS), but the procedure-related complications of RFA were significantly less than those of rHR. Among the subgroups with Milan criteria, rHR was similar to RFA in 1-, 3-, and 5-year OS and 1-year DFS, but superior to RFA in 3- and 5-year DFS.

Conclusions: RFA is the first choice for recurrent HCC meeting Milan criteria. When it does not meet the Milan criteria, minimally invasive treatment should not be carried out at the cost of survival, and rHR should be the first choice.
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http://dx.doi.org/10.1080/13645706.2020.1839775DOI Listing
November 2020

Combined effects of rice straw-derived biochar and water management on transformation of chromium and its uptake by rice in contaminated soils.

Ecotoxicol Environ Saf 2021 Jan 26;208:111506. Epub 2020 Oct 26.

State Key Laboratory for Quality and Safety of Agro-products; Key Laboratory of Information Traceability for Agricultural Products, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

Chromium (Cr) pollution in soil is a global problem owing to its wide industrial use. The mobility, toxicity, and crop uptake of Cr depends on its valence state. Cr(VI) is highly mobile and toxic whereas Cr(III) is generally considered immobile and less toxic. We performed a pot experiment to investigate the combined effects of rice straw-derived biochar and water management on transformation of Cr and its uptake by rice in contaminated soils. The main plots had water management treatments of alternating wetting and drying (AWD) and continuous flooding (CF), and the subplots had three levels of straw biochar (0, 5, and 10 g kg). The results showed that water management and the addition of biochar had a significant effect on the dynamics of soil redox potential (Eh), pH, dissolved organic carbon (DOC), and Fe(II) concentration. As these parameters are important factors affecting Cr transformation in paddy soils, the dynamics of the Cr(III) and Cr(VI) concentrations were clearly different under different treatments. The highest reduction of Cr(VI) was observed in the treatment with CF water management in combination with 10 g kg of biochar amendment, which resulted in a 62% reduction of Cr(VI) to Cr(III) in soil. The alterations in the oxidation state of Cr greatly affected its accumulation in the rice grains. The CF combined with 10 g kg of biochar treatment, caused the Cr concentration in rice grains to be 66.2% lower compared with that of the unamended control under AWD water management. Possibly owing to the reduction in phytotoxic effects of Cr(VI), the combined treatment showed an improvement in rice grain weight. In conclusion, the combination of 10 g kg of biochar amendment and CF water management may potentially be used in Cr-contaminated soil to mitigate the impacts of Cr contamination on rice production.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111506DOI Listing
January 2021

Reducing bioavailability of heavy metals in contaminated soil and uptake by maize using organic-inorganic mixed fertilizer.

Chemosphere 2020 Dec 28;261:128122. Epub 2020 Aug 28.

College of Ecology & Environment, Hainan University, Haikou, Hainan, 570228, PR China; State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, Hainan, 570228, PR China; Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province, Hainan University, Haikou, Hainan, 570228, PR China; Center for Eco-Environmental Restoration Engineering of Hainan Province, Hainan University, Haikou, Hainan, 570228, PR China; Key Laboratory for Environmental Toxicology of Haikou, Hainan University, Haikou, Hainan, 570228, PR China. Electronic address:

Heavy metals in soil are harmful to human health via the food chain, but little is known about the mechanism of reducing bioavailability of Cd or Pb to maize (Zea mays L.) by applying complex amendments to soil. A field experiment was conducted at a tropical site in Hainan Province, China, that had been subjected to soil pollution by Cd and Pb from past mining activities. There were ten treatment groups comprising a mixture of biochar, hydroxyapatite (HAP), manure, and plant ash in varying proportions and at three different rates. Compared with untreated soil, all treatments increased pH by 2-3 units in bulk soil or 1-2 units in rhizosphere soil. For all amendments, the concentration of Cd in all parts of maize plants was decreased compared with unamended soil, but this effect was much smaller for Pb. The greatest effect was found with a mixture containing the ratio of HAP:manure:biochar:plant ash as 6:4:2:1 when applied at 20.1 t ha. The dominant microbial group in contaminated soil was Proteobacteria. There is evidence that this group can immobilize Cd by mechanisms that include biosorption and bioprecipitation. It was concluded that the mixed amendments containing biochar, HAP, manure, and plant ash can be useful in decreasing Cd uptake by maize. The amendment in this study likely operates through a combination of soil chemical changes and by influencing the soil-microbe-plant interaction.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128122DOI Listing
December 2020

LINC01106 drives colorectal cancer growth and stemness through a positive feedback loop to regulate the Gli family factors.

Cell Death Dis 2020 10 16;11(10):869. Epub 2020 Oct 16.

Department of General Surgery, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, 230001, Hefei, Anhui Province, P. R. China.

Long non-coding RNAs (lncRNAs) are essential contributors to the progression of various human cancers. Long intergenic non-protein coding RNA 1106 is a member of lncRNAs family. Until now, the specific role of LINC01106 in CRC remains undefined. The aim the current study was to unveil the functions of LINC01106 and explore its potential molecular mechanism in CRC. Based on the data of online database GEPIA, we determined that LINC01106 was expressed at a high level in colon adenocarcinoma (COAD) tissues compared to normal colon tissues. More importantly, high level of LINC01106 had negative correlation with the overall survival of COAD patients. Additionally, we also determined the low level of LINC01106 in normal colon tissues based on UCSC database. Through qRT-PCR, we identified that LINC01106 was highly expressed in CRC tissues compared to adjacent normal ones. Similarly, we detected the expression of LINC01106 and confirmed that LINC01106 was expressed higher in CRC cells than that in normal cells. Subsequently, LINC01106 was mainly distributed in the cytoplasm. LINC01106 induced the proliferation, migration, and stem-like phenotype of CRC cells. Mechanistically, cytoplasmic LINC01106 positively modulated Gli4 in CRC cells by serving as a miR-449b-5p sponge. Furthermore, nuclear LINC01106 could activate the transcription of Gli1 and Gli2 through recruiting FUS to Gli1 and Gli2 promoters. Mechanism of investigation unveiled that Gli2 was a transcription activator of LINC01106. In conclusion, Gli2-induced upregulation of LINC01106 aggravates CRC progression through upregulating Gli2, Gli2, and Gli4.
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http://dx.doi.org/10.1038/s41419-020-03026-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567881PMC
October 2020

Structural Features and PF4 Functions that Occur in Heparin-Induced Thrombocytopenia (HIT) Complicated by COVID-19.

Antibodies (Basel) 2020 Oct 10;9(4). Epub 2020 Oct 10.

Departments of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19301, USA.

Platelet factor 4 (PF4, CXCL4) is a small chemokine protein released by activated platelets. Although a major physiological function of PF4 is to promote blood coagulation, this cytokine is involved in innate and adaptive immunity in events when platelets are activated in response to infections. Coronavirus disease 2019 (COVID-19) patients have abnormal coagulation activities, and severe patients develop higher D-dimer levels. D-dimers are small protein products present in the blood after blood clots are degraded by fibrinolysis. To prevent clotting, heparin is often clinically used in COVID-19 patients. Some clinical procedures for the management of COVID-19 patients may include extracorporeal membrane oxygenation (ECMO) and renal replacement therapy (CRRT), which also require the use of heparin. Anti-PF4 antibodies are frequently detected in severe patients and heparin-induced thrombocytopenia (HIT) can also be observed. PF4 and its role in HIT as well as in pathologies seen in COVID-19 patients define a potential therapeutic option of using blocking antibodies in the treatment of COVID-19.
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http://dx.doi.org/10.3390/antib9040052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709132PMC
October 2020

Improved wet shear strength in eco-friendly starch-cellulosic adhesives for woody composites.

Carbohydr Polym 2020 Dec 10;250:116884. Epub 2020 Aug 10.

National Forestry and Grassland Administration Key Laboratory of Plant Fiber Functional Materials, College of Materials Engineering, Fujian Agriculture and Forestry University, 350002, Fuzhou, China. Electronic address:

Development of eco-friendly adhesives from renewable biomass has attracted considerable attention in recent years. Here, we present a novel approach via combination of waste newspaper (WNP) powder, oxidized glutinous rice starch, and polyamidoamine-epichlorohydrin (PAE) to prepare a formaldehyde-free starch-cellulosic adhesive (SCA) for woody composites. The oxidation treatment made the carboxyl/carbonyl groups more available in starch. Plywood bonded by the optimum SCA with 50 wt% of the WNP powder showed a wet shear strength of 0.83 MPa exceeding that of the oxidized starch adhesive by 130.5 %. During the curing process of SCA, the oxidized starch and WNP fiber participated into the crosslinking reaction with PAE via ester and ether bonds, as evidenced by FTIR analysis. The resulting cured adhesive had enhanced crystalline structures, thermal properties, hydrophobicity, wet-cohesion, rheological properties, and adhesiveness to wood. The SCA showed great potential in wood composites as an alternative to formaldehyde-derived adhesives.
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http://dx.doi.org/10.1016/j.carbpol.2020.116884DOI Listing
December 2020

Does intraoperative indocyanine green fluorescence angiography decrease the incidence of anastomotic leakage in colorectal surgery? A systematic review and meta-analysis.

Int J Colorectal Dis 2021 Jan 18;36(1):57-66. Epub 2020 Sep 18.

Department of General Surgery, Anhui Provincial Hospital Affiliated to the Anhui Medical University, Hefei, China.

Background: Colorectal anastomoses in patients with colorectal cancer carry a high risk of leakage. Indocyanine green fluorescence angiography (ICG-FA) is a new technique that allows surgeons to assess the blood perfusion of the anastomosis during operation. This meta-analysis aimed to evaluate whether ICG-FA could prevent anastomotic leakage (AL) in colorectal surgery.

Methods: Four databases (PubMed, Embase, Web of Science, and Cochrane Library) were searched to identify suitable literatures until March 2020 that compared AL rates between intraoperative use and non-use of ICG-FA in colorectal surgery for cancer. The Review Manager 5.3 software was used to perform the statistical analysis. Evaluation of articles quality and analysis for publication bias were also conducted.

Results: Thirteen studies of 4037 patients were included in the meta-analysis. The study included 1806 patients in the ICG group and 2231 patients in the control group. The pooled incidence of AL in ICG group was 3.8% compared with 7.8% in control group. There was a significant difference in AL rate with or without use of ICG-FA (OR 0.44; 95% CI 0.33-0.59; P < 0.00001). Reoperation rates were 2.6% and 6.9% in ICG and control groups, respectively. Application of intraoperative ICG-FA was associated with a lower risk of reoperation (OR 0.39; 95% CI 0.16-0.94; P = 0.04). Overall complication rate was 15.6% in the ICG group compared with 21.2% in the control group. Overall complications were significantly reduced when using ICG-FA (OR 0.62; 95% CI 0.47-0.82; P = 0.0008). Mortality rate was not statistically different with or without the use of ICG-FA (OR 1.22; 95% CI 0.20-7.30; P = 0.83).

Conclusion: The results revealed that ICG-FA reduced risks of AL, reoperation, and overall complications for colorectal cancer patients undergoing colorectal surgery. Well-designed RCTs are needed to confirm the usefulness of intraoperative ICG-FA for preventing surgical complications like AL and reoperation.
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http://dx.doi.org/10.1007/s00384-020-03741-5DOI Listing
January 2021

Immune characteristics of severe and critical COVID-19 patients.

Signal Transduct Target Ther 2020 08 31;5(1):179. Epub 2020 Aug 31.

Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, Henan, China.

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http://dx.doi.org/10.1038/s41392-020-00296-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456639PMC
August 2020

Laboratory verification of an RT-PCR assay for SARS-CoV-2.

J Clin Lab Anal 2020 Oct 4;34(10):e23507. Epub 2020 Aug 4.

Department of Clinical Laboratory, Fourth Affiliated Hospital of Zhejiang University School of Medicine, Jinhua, China.

Background: Reverse transcription-polymerase chain reaction (RT-PCR) is an extremely common clinical method for detecting pathogens, particularly for emerging infectious diseases such as the new coronavirus disease (COVID-19). Currently, detection of the RNA from the novel coronavirus SARS-CoV-2 is the gold standard for establishing a COVID-19 diagnosis. This study evaluates the characteristic performance of the analytical system in a clinical laboratory.

Methods: A commercial SARS-CoV-2 RNA RT-PCR Kit used in a clinical laboratory is assessed based on ISO 15189 verification requirements. A multiple real-time RT-PCR assay for the RdRP, N, and E genes in SARS-CoV-2 is verified.

Results: The analytical system exhibits good analytical sensitivity (1000 copies/mL) and specificity (100%); however, the values of 86.7% and 100% for analytical accuracy deserved attention, compared with two other types of methods. Overall, the kit is potentially useful for SARS-CoV-2 diagnostic testing and meets the verification requirements.

Conclusion: Compliance with international standards, such as ISO 15189, is valuable for clinical laboratories and for improving laboratory medicine quality and safety. Normalization is essential for obtaining reliable results from the SARS-CoV-2 RNA RT-PCR assay. This study aims to develop an improved SARS-CoV-2 verification framework compared with traditional molecular diagnostic methods, given the urgency of implementing new assays in clinical laboratories.
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http://dx.doi.org/10.1002/jcla.23507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435359PMC
October 2020

Cooperation and Competition among information on social networks.

Sci Rep 2020 07 22;10(1):12160. Epub 2020 Jul 22.

College of Science, Huazhong Agricultural University, Wuhan, 430070, China.

When multiple information are spread on social networks, there may be Cooperation and Competition among these information. Based on a new spreading model of multiple information, we studied Cooperation and Competition in information spreading, and analyzed the influence of different factors on Cooperation and Competition. Through a large number of computer simulation experiments, we found that: (1) when multiple information are spread on social networks, there is Cooperation and Competition among these information; (2) the smaller the distance between two information sources is, the stronger the Cooperation and Competition among these information are; (3) the greater the value of social reinforcement is, the stronger the Cooperation and Competition among these information are; (4) the weaker the human heterogeneity of one information is, the stronger the Cooperation and Competition among this information and other information are.
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http://dx.doi.org/10.1038/s41598-020-69098-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376155PMC
July 2020

Mechanically Reinforced Localized Structure Design to Stabilize Solid-Electrolyte Interface of the Composited Electrode of Si Nanoparticles and TiO Nanotubes.

Small 2020 Jul 11;16(30):e2002094. Epub 2020 Jun 11.

Innovative Centre for Flexible Devices (iFLEX), School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.

Silicon anode with extremely high theoretical specific capacity (≈4200 mAh g ), experiences huge volume changes during Li-ion insertion and extraction, causing mechanical fracture of Si particles and the growth of a solid-electrolyte interface (SEI), which results in a rapid capacity fading of Si electrodes. Herein, a mechanically reinforced localized structure is designed for carbon-coated Si nanoparticles ([email protected]) via elongated TiO nanotubes networks toward stabilizing Si electrode via alleviating mechanical strain and stabilizing the SEI layer. Benefited from the rational localized structure design, the carbon-coated Si nanoparticles/TiO nanotubes composited electrode ([email protected]/TiNT) exhibits an ideal electrode thickness swelling, which is lower than 1% after the first cycle and increases to about 6.6% even after 1600 cycles. While for traditional [email protected]/carbon nanotube composited electrode, the initial swelling ratio is about 16.7% and reaches ≈190% after 1600 cycles. As a result, the [email protected]/TiNT electrode exhibits an outstanding capacity of 1510 mAh g at 0.1 A g with high rate capability and long-time cycling performance with 95% capacity retention after 1600 cycles. The rational design on mechanically reinforced localized structure for silicon electrode will provide a versatile platform to solve the current bottlenecks for other alloyed-type electrode materials with large volume expansion toward practical applications.
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http://dx.doi.org/10.1002/smll.202002094DOI Listing
July 2020

Characteristic of Five Subpopulation Leukocytes in Single-Cell Levels Based on Partial Principal Component Analysis Coupled with Raman Spectroscopy.

Appl Spectrosc 2020 Dec 12;74(12):1463-1472. Epub 2020 Oct 12.

Department of Laboratory Medicine, Southwest Hospital, Chongqing, China.

Characteristics of five subpopulation leukocytes in single-cell levels based on partial principal component analysis coupled with Raman spectroscopy were proposed to recognize the biochemical features of five subpopulation leukocytes. Using wavelet transform, the reconstructed spectra of the low-frequency wavelet coefficients were used to perform multiple principal component analysis based on segmented spectral data wreathing cover at 720-800 cm, 840-994 cm, and 1010-1070 cm wavenumbers, respectively. Our approach is promising since it enables to establish a better understanding of the underlying molecular difference between the subtypes of leukocytes in a label-free manner and to estimate the source of infection.
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http://dx.doi.org/10.1177/0003702820938069DOI Listing
December 2020

Magnetic Targeting and Ultrasound Activation of Liposome-Microbubble Conjugate for Enhanced Delivery of Anticancer Therapies.

ACS Appl Mater Interfaces 2020 May 15;12(21):23737-23751. Epub 2020 May 15.

Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, Anhui 230027, P. R. China.

Effective delivery of chemotherapeutics with minimal toxicity and maximal outcome is clinically important but technically challenging. Here, we synthesize a complex of doxorubicin (DOX)-loaded magneto-liposome (DOX-ML) microbubbles (DOX-ML-MBs) for magnetically responsive and ultrasonically sensitive delivery of anticancer therapies with enhanced efficiency. Citrate-stabilized iron oxide nanoparticles (MNs) of 6.8 ± 1.36 nm were synthesized, loaded with DOX in the core of oligolamellar vesicles of 172 ± 9.2 nm, and covalently conjugated with perfluorocarbon (PFC)-gas-loaded microbubbles to form DOX-ML-MBs of ∼4 μm. DOX-ML-MBs exhibited significant magnetism and were able to release chemotherapeutics and DOX-MLs instantly upon exposure to ultrasound (US) pulses. In vitro studies showed that DOX-ML-MBs in the presence of US pulses promoted apoptosis and were highly effective in killing both BxPc-3 and Panc02 pancreatic cancer cells even at a low dose. Significant reduction in the tumor volume was observed after intravenous administration of DOX-ML-MBs in comparison to the control group in a pancreatic cancer xenograft model of nude mice. Deeply penetrated iron oxide nanoparticles throughout the magnetically targeted tumor tissues in the presence of US stimulation were clearly observed. Our study demonstrated the potential of using DOX-ML-MBs for site-specific targeting and controlled drug release. It opens a new avenue for the treatment of pancreatic cancer and other tissue malignancies where precise delivery of therapeutics is necessary.
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http://dx.doi.org/10.1021/acsami.0c05308DOI Listing
May 2020