Publications by authors named "Zhiqiang Zhang"

587 Publications

GPC3 affects the prognosis of lung adenocarcinoma and lung squamous cell carcinoma.

BMC Pulm Med 2021 Jun 10;21(1):199. Epub 2021 Jun 10.

Molecular Oncology Department of Cancer Research Institution, The First Hospital of China Medical University, Nanjingbei Street, Heping District, Shenyang, 110001, Liaoning Province, China.

Background: Glypican 3 (GPC3) is a heparin sulphate proteoglycan whose expression is associated with several malignancies. However, its expression in non-small-cell lung carcinoma (NSCLC) is limited and ambiguous. This study aimed to comprehensively evaluate the expression of GPC3 in NSCLC and develop a risk-score model for predicting the prognosis of NSCLC.

Methods: The gene expression profiles of lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) were downloaded from the UCSC Xena database. Using the limma package, the differentially expressed genes (DEGs) between different comparison groups were analysed and the differential expression of GPC3 was calculated. A functional enrichment analysis was conducted for GPC3-associated genes using the DAVID tool. For the GPC3-associated genes shared by the four comparison groups, a protein-protein interaction network was built using the Cytoscape software. After conducting a survival analysis and a Cox regression analysis, the genes found to be significantly correlated with prognosis were selected to construct a risk-score model. Besides, the gene and protein levels of GPC3 were examined by quantitative reverse transcriptase-PCR (qRT-PCR) and immunohistochemistry (IHC) in LUSC tissues and paracancer tissues.

Results: The differential expression of GPC3 was significant (adjusted P < 0.05) in the NSCLC vs. normal, LUAD vs. normal, LUSC versus normal, and LUAD versus. LUSC comparison groups. GPC3 directly interacted with SERPINA1, MFI2, and FOXM1. Moreover, GPC3 expression was significantly correlated with pathologic N, pathologic T, gender, and tumour stage in LUAD samples. Finally, the risk-score model (involving MFI2, FOXM1, and GPC3) for LUAD and that (involving SERPINA1 and FOXM1) for LUSC were established separately. The qRT-PCR result showed that GPC3 expression was much higher in the LUSC tissues than that in the normal group. The IHC results further showed that GPC3 is highly expressed in LUSC tissues, but low in paracancer tissues.

Conclusion: The three-gene risk-score model for LUAD and the two-gene risk-score model for LUSC might be valuable in improving the prognosis of these carcinomas.
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http://dx.doi.org/10.1186/s12890-021-01549-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194200PMC
June 2021

Natural Molybdenite- and Tyrosinase-Based Amperometric Catechol Biosensor Using Acridine Orange as a Glue, Anchor, and Stabilizer for the Adsorbed Tyrosinase.

ACS Omega 2021 Jun 18;6(21):13719-13727. Epub 2021 May 18.

Department of Life Science and Green Chemistry, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan.

To develop a natural mineral-based electrochemical enzyme biosensor, natural molybdenite (MLN), tyrosinase (TYR), and acridine orange (AO) were coadsorbed onto a glassy carbon electrode (GCE). The developed TYR/AO/MLN-GCE-based amperometric TYR biosensor exhibited excellent performance for highly sensitive determination of catechol (linear range, 0.1-80 μM; sensitivity, 0.0315 μA/μM; LOD, 0.029 μM; response time, <4 s) with good reproducibility and good operational and storage stabilities. The electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance with dissipation (QCM-D) revealed interesting roles of AO: (1) an efficient glue for enhancing the amount of the adsorbed TYR on the MLN-GCE, (2) an anchor for efficient orientation of the adsorbed TYR on the MLN-GCE, and (3) a stabilizer providing a suitable microenvironment for the adsorbed TYR on the MLN-GCE surface. This physical adsorption-based AO-coupled enzyme-modification strategy onto natural MLN would be a versatile strategy to develop cost-effective and environment-friendly natural mineral-based electrochemical biosensors and bioelectronic devices.
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http://dx.doi.org/10.1021/acsomega.1c00973DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173569PMC
June 2021

LECT2 Protects Nile Tilapia () Against Infection.

Front Immunol 2021 20;12:667781. Epub 2021 May 20.

College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, China.

Leukocyte cell-derived chemotaxin 2 (LECT2) is a multifunctional cytokine that especially plays an important role in innate immune. However, the roles of LECT2 in the immune response of the economically important fish Nile tilapia () against bacterial infection remains unclear. In this study, a gene from Nile tilapia () was identified, and its roles in the fish's immune response against bacterial infection were determined and characterised. contains an open reading frame of 456 bp that encodes a peptide of 151 amino acids, as well as the conservative peptidase M23 domain. On-LECT2 is 62%-84% identical to other fish species and about 50% identical to mammals. The highest transcriptional level of was detected in the liver, whereas the lowest levels were detected in the other tissues. Moreover, the On-LECT2 protein is located mainly in the brain and head kidney. The transcriptional levels of substantially increased in the head kidney, brain, liver and spleen after infection. Knockdown led to higher mortality due to liver necrosis or haemorrhage and splenomegaly. analysis indicated that the recombinant protein of On-LECT2 improved phagocytic activity of head kidney-derived macrophages. challenge experiments revealed several functions of On-LECT2 in the immune response of Nile tilapia against bacterial infection, including promotion of inflammation, reduction of tissue damages and improvement of survival rate.
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http://dx.doi.org/10.3389/fimmu.2021.667781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174566PMC
May 2021

Impact of various vegetation configurations on traffic fine particle pollutants in a street canyon for different wind regimes.

Sci Total Environ 2021 May 23;789:147960. Epub 2021 May 23.

State Key Laboratory of Environmental Criteria and Risk Assessment, State Environmental Protection Key Laboratory of Regional Eco-process and Function Assessment, Chinese Research Academy of Environmental Sciences, Dayang Fang 8, Chaoyang District, Beijing 100012, China; Institute of Ecological Environment, Chinese Research Academy of Environmental Sciences, Dayang Fang 8, Chaoyang District, Beijing 100012, China.

Vegetation establishment in urban areas is a potential solution to combat elevated particulate matter (PM) pollution, create cleaner environment for residents and enhance the sustainability of cities. However, vegetation effect at the points of interest in street-canyon on traffic pollutant from multiple interconnected factors (e.g., plant species, vegetation configurations, aerodynamic effect, deposition effect and complex wind regimes) is still not well studied. Therefore, taking roadside vegetation and street canyon as research objects, we evaluated vegetation effect (VE) for vegetation configurations (VCs) with several tree species on the dispersion, deposition, and distribution of traffic generated PM pollutant under different wind regimes. Results showed that (1) the transportation and distribution of traffic PM pollutant were different from wind regimes; (2) total VEs varied from -88.3% to 25.5%, depending on different VCs and wind regimes; perpendicular wind had the best VEs, while oblique wind had the worst VEs among the three wind directions; VEs of cypress were better than pine and poplar; VEs of one side planting were better than two sides planting. (3) the optimal VCs were found by each wind direction; two sides planting by shrub was suitable for parallel and oblique winds; for the perpendicular wind, the optimal VC was that two sides planting by cypress-shrub and increased canopy volume in the street center; and (4) VE were significantly correlated (P < 0.05) with vegetation parameters at lower wind speed, however, no correlations were found at higher wind speed under parallel wind; leeward wall VEs were significantly correlated with aerodynamic parameter (P < 0.001) while windward wall VEs and pedestrian-level VEs with deposition parameter (P < 0.05) under perpendicular wind; VEs were significantly decreased (P < 0.001) with aerodynamic parameter under oblique wind. The study highlights the impact of urban vegetation on air environment and provides insights for vegetation establishment from the viewpoint of improving air quality.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147960DOI Listing
May 2021

Long noncoding HOXA11-AS knockdown suppresses the progression of non-small cell lung cancer by regulating miR-3619-5p/SALL4 axis.

J Mol Histol 2021 May 29. Epub 2021 May 29.

Department of Thoracic Surgery, Qingpu Branch of Zhongshan Hospital Affiliated To Fudan University, No.1158, Gongyuan East Road, Qingpu District, Shanghai, 210700, China.

Accumulating evidence suggested that many long noncoding RNAs (lncRNAs) were widely involved in the development and progression of non-small cell lung cancer (NSCLC). However, the roles of lncRNA homeobox A11 antisense (HOXA11-AS) and its underlying mechanism in NSCLC remains largely unknown. The expression levels of HOXA11-AS, miR-3619-5p and sal-like protein 4 (SALL4) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot analysis was used to measure the protein levels of hexokinase II (HK2) and SALL4. Cell proliferation, apoptosis, migration and invasion were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry and transwell assay, respectively. The glucose consumption and lactate production were measured using glucose assay kit and lactate assay kit, respectively. The potential binding sites between miR-3619-5p and HOXA11-AS or SALL4 were predicted by online software and verified by luciferase report assay. A xenograft tumor model was established to confirm the function of HOXA11-AS in NSCLC in vivo. HOXA11-AS and SALL4 were upregulated while miR-3619-5p was downregulated in NSCLC tissues and cells. HOXA11-AS knockdown suppressed cell proliferation, migration, invasion, and glycolysis but promoted apoptosis in NSCLC cells. Moreover, miR-3619-5p could directly bind to HOXA11-AS and its inhibition attenuated the inhibitory effect of HOXA11-AS knockdown on progression of NSCLC cells. Furthermore, SALL4 was a direct target of miR-3619-5p and its overexpression reversed the anti-tumor role of miR-3619-5p in NSCLC cells. Besides, HOXA11-AS modulated SALL4 expression via sponging miR-3619-5p. Additionally, silencing HOXA11-AS inhibited tumor growth though upregulating miR-3619-5p and downregulating SALL4. Collectively, HOXA11-AS knockdown inhibited the progression of NSCLC by regulating miR-3619-5p/SALL4 axis, which might offer a novel avenue for interpreting the mechanism of NSCLC development.
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http://dx.doi.org/10.1007/s10735-021-09981-1DOI Listing
May 2021

Modulation of gut microbiota by chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats.

Carbohydr Polym 2021 Aug 24;266:118099. Epub 2021 Apr 24.

University of Liege-Gembloux Agro-Bio Tech, Laboratory of Biomass and Green Technologies, Passage des déportés 2, B-5030 Gembloux, Belgium.

Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo. Here, the effects of CSCa on osteoporosis rats were investigated. Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum. 16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces. The correlation analysis showed some gut microbiota taxa were significantly correlated with osteoporosis phenotypes and the enriched metabolites. Taken together, dietary CSCa intervention has the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats. This study provides some scientific evidence for the potential effects of CSCa as the food supplement on the osteoporosis.
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http://dx.doi.org/10.1016/j.carbpol.2021.118099DOI Listing
August 2021

Delayed brain development of Rolandic epilepsy profiled by deep learning-based neuroanatomic imaging.

Eur Radiol 2021 May 20. Epub 2021 May 20.

Department of Medical Imaging, Jinling Hospital, the First School of Clinical Medicine, Southern Medical University, Nanjing, China.

Objectives: Although Rolandic epilepsy (RE) has been regarded as a brain developmental disorder, neuroimaging studies have not yet ascertained whether RE has brain developmental delay. This study employed deep learning-based neuroanatomic biomarker to measure the changed feature of "brain age" in RE.

Methods: The study constructed a 3D-CNN brain age prediction model through 1155 cases of typically developing children's morphometric brain MRI from open-source datasets and further applied to a local dataset of 167 RE patients and 107 typically developing children. The brain-predicted age difference was measured to quantitatively estimate brain age changes in RE and further investigated the relevancies with cognitive and clinical variables.

Results: The brain age estimation network model presented a good performance for brain age prediction in typically developing children. The children with RE showed a 0.45-year delay of brain age by contrast with typically developing children. Delayed brain age was associated with neuroanatomic changes in the Rolandic regions and also associated with cognitive dysfunction of attention.

Conclusion: This study provided neuroimaging evidence to support the notion that RE has delayed brain development.

Key Points: • The children with Rolandic epilepsy showed imaging phenotypes of delayed brain development with increased GM volume and decreased WM volume in the Rolandic regions. • The children with Rolandic epilepsy had a 0.45-year delay of brain-predicted age by comparing with typically developing children, using 3D-CNN-based brain age prediction model. • The delayed brain age was associated with morphometric changes in the Rolandic regions and attentional deficit in Rolandic epilepsy.
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http://dx.doi.org/10.1007/s00330-021-08048-9DOI Listing
May 2021

Identification of a DNA Methylation-Based Prognostic Signature for Patients with Triple-Negative Breast Cancer.

Med Sci Monit 2021 May 18;27:e930025. Epub 2021 May 18.

Department of Breast Surgery, Capital Medical University Electric Power Teaching Hospital, Beijing, China (mainland).

BACKGROUND Aberrant DNA methylation is an important biological regulatory mechanism in malignant tumors. However, it remains underutilized for establishing prognostic models for triple-negative breast cancer (TNBC). MATERIAL AND METHODS Methylation data and expression data downloaded from The Cancer Genome Atlas (TCGA) were used to identify differentially methylated sites (DMSs). The prognosis-related DMSs were selected by univariate Cox regression analysis. Functional enrichment was analyzed using DAVID. A protein-protein interaction (PPI) network was constructed using STRING. Finally, a methylation-based prognostic signature was constructed using LASSO method and further validated in 2 validation cohorts. RESULTS Firstly, we identified 743 DMSs corresponding to 332 genes, including 357 hypermethylated sites and 386 hypomethylated sites. Furthermore, we selected 103 prognosis-related DMSs by univariate Cox regression. Using a LASSO algorithm, we established a 5-DMSs prognostic signature in TCGA-TNBC cohort, which could classify TNBC patients with significant survival difference (log-rank p=4.97E-03). Patients in the high-risk group had shorter overall survival than patients in the low-risk group. The excellent performance was validated in GSE78754 (HR=2.42, 95%CI: 1.27-4.59, log-rank P=0.0055). Moreover, for disease-free survival, the prognostic performance was verified in GSE141441 (HR=2.09, 95%CI: 1.28-3.44, log-rank P=0.0027). Multivariate Cox regression analysis indicated that the 5-DMSs signature could serve as an independent risk factor. CONCLUSIONS We constructed a 5-DMSs signature with excellent performance for the prediction of disease-free survival and overall survival, providing a guide for clinicians in directing personalized therapeutic regimen selection of TNBC patients.
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http://dx.doi.org/10.12659/MSM.930025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140526PMC
May 2021

Identification of poly(ADP-ribose) polymerase 9 (PARP9) as a noncanonical sensor for RNA virus in dendritic cells.

Nat Commun 2021 05 11;12(1):2681. Epub 2021 May 11.

Department of Surgery and Immunobiology and Transplant Science Center, Houston Methodist, Houston, TX, USA.

Innate immune cells are critical in protective immunity against viral infections, involved in sensing foreign viral nucleic acids. Here we report that the poly(ADP-ribose) polymerase 9 (PARP9), a member of PARP family, serves as a non-canonical sensor for RNA virus to initiate and amplify type I interferon (IFN) production. We find knockdown or deletion of PARP9 in human or mouse dendritic cells and macrophages inhibits type I IFN production in response to double strand RNA stimulation or RNA virus infection. Furthermore, mice deficient for PARP9 show enhanced susceptibility to infections with RNA viruses because of the impaired type I IFN production. Mechanistically, we show that PARP9 recognizes and binds viral RNA, with resultant recruitment and activation of the phosphoinositide 3-kinase (PI3K) and AKT3 pathway, independent of mitochondrial antiviral-signaling (MAVS). PI3K/AKT3 then activates the IRF3 and IRF7 by phosphorylating IRF3 at Ser385 and IRF7 at Ser437/438 mediating type I IFN production. Together, we reveal a critical role for PARP9 as a non-canonical RNA sensor that depends on the PI3K/AKT3 pathway to produce type I IFN. These findings may have important clinical implications in controlling viral infections and viral-induced diseases by targeting PARP9.
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http://dx.doi.org/10.1038/s41467-021-23003-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113569PMC
May 2021

Mild hypothermia facilitates mitochondrial transfer from astrocytes to injured neurons during oxygen-glucose deprivation/reoxygenation.

Neurosci Lett 2021 Jun 7;756:135940. Epub 2021 May 7.

Department of Anesthesiology, Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China. Electronic address:

Mitochondrial dysfunction is now considered an important sign of neuronal death during cerebral ischemia/reperfusion (I/R) injury. Studies have shown that the transfer of mitochondria from astrocytes to injured neurons contributes to endogenous neuroprotection after stroke. Basic and clinical studies have shown that mild hypothermia exerts a clear protective effect on neurons after cerebral ischemic injury, but the role of mild hypothermia in this endogenous neuroprotective mechanism remains unclear. Here, we established a neuronal cell oxygen-glucose deprivation (OGD)/reoxygenation (OGD/R)-induced injury model and explored the effect of mild hypothermia on the transfer of mitochondria from astrocytes to injured neurons. Astrocytes in the hypothermia group (33 °C) released more functional mitochondria into the extracellular medium than those in the normal temperature group (37 °C). Compared with cells in the normal temperature group, OGD-injured neuronal cells in the mild hypothermia group exhibited an increased intracellular ATP content, mitochondrial membrane potential (MMP) and cellular viability and a decreased death rate after the addition of astrocyte-derived conditioned medium. Based on the results of this study, mild hypothermia promotes endogenous neuroprotective effects through a mechanism related to functional mitochondria released from astrocytes into the extracellular space and transferred into injured neurons.
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http://dx.doi.org/10.1016/j.neulet.2021.135940DOI Listing
June 2021

BCCT: A GUI Toolkit for Brain Structural Covariance Connectivity Analysis on MATLAB.

Front Hum Neurosci 2021 20;15:641961. Epub 2021 Apr 20.

College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, China.

Brain structural covariance network (SCN) can delineate the brain synchronized alterations in a long-range time period. It has been used in the research of cognition or neuropsychiatric disorders. Recently, causal analysis of structural covariance network (CaSCN), winner-take-all and cortex-subcortex covariance network (WTA-CSSCN), and modulation analysis of structural covariance network (MOD-SCN) have expended the technology breadth of SCN. However, the lack of user-friendly software limited the further application of SCN for the research. In this work, we developed the graphical user interface (GUI) toolkit of brain structural covariance connectivity based on MATLAB platform. The software contained the analysis of SCN, CaSCN, MOD-SCN, and WTA-CSSCN. Also, the group comparison and result-showing modules were included in the software. Furthermore, a simple showing of demo dataset was presented in the work. We hope that the toolkit could help the researchers, especially clinical researchers, to do the brain covariance connectivity analysis in further work more easily.
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http://dx.doi.org/10.3389/fnhum.2021.641961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093864PMC
April 2021

Anti-seizure medication correlated changes of cortical morphology in childhood epilepsy with centrotemporal spikes.

Epilepsy Res 2021 Jul 23;173:106621. Epub 2021 Mar 23.

Department of Medical Imaging, Jinling Hospital, The First School of Clinical Medicine, Southern Medical University, Nanjing, 210002, China; Department of Medical Imaging, Jinling Hospital, Nanjing University School of Medicine, Nanjing, 210002, China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing, 210093, China; Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, 149 Thirteenth Street, Suite 2301, Charlestown, MA, 02129, USA. Electronic address:

To investigate the morphological changes of cerebral cortex correlating with anti-seizure medication in Childhood Epilepsy with Centrotemporal Spikes (CECTS), and their relationships with seizure control. This study included a total of 188 children, including 62 patients with CECTS taking anti-seizure drugs, 56 patients with drug-naive, and 70 healthy controls. A portion of cases were also followed-up for longitudinal analysis. Cortical morphological parameters were quantitatively measured by applying surface-based morphometry analysis to high-resolution three-dimension T1 weighted images. Among the three groups, the morphological indices were compared to quantify any cortical changes affected by seizures and medication. The relationships among anti-seizure medication, seizure controls and cortical morphometry were investigated using causal mediator analysis. The Rolandic cortex of the drug-naive patients showed abnormal cortical thickness by comparing with that of healthy controls, and thinning by comparing with that of patients with medication. The cortical thickness in the Rolandic regions was negatively correlated with duration of medication and duration of seizure-free. Longitudinal analysis further demonstrated that the thickness of Rolandic cortex thinned in post-medication state relative to the pre-medication state. Mediation analysis revealed that morphological alteration of the Rolandic cortex might act as a mediator in the path of anti-seizure medication on seizure control. Our findings highlighted that anti-seizure medication was associated with regression of abnormal increment of cortical thickness in the Rolandic regions in CECTS. The neuroanatomical alteration might be a mediating factor in the process of seizure control by anti-seizure medication.
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http://dx.doi.org/10.1016/j.eplepsyres.2021.106621DOI Listing
July 2021

A Dual-mode 2D Matrix Array for Ultrasound Image-guided Noninvasive Therapy.

IEEE Trans Biomed Eng 2021 Apr 19;PP. Epub 2021 Apr 19.

Focused ultrasound (FUS) lacks reliable real-time image guidance, which hinders the development of non-invasive ultrasound treatment in many important clinical applications. A dual-mode ultrasound array, capable of both imaging and therapy offers a new and reliable strategy for image-guided ultrasound therapy applications. The strategy has the advantages of real-time use, low cost, portability and inherent registration between imaging and therapeutic coordinate systems. In this work, a dual-mode two-dimensional (2D) matrix array with 1 MHz center frequency and 256 elements for ultrasound image-guided non-invasive therapy is reported. The array can provide three-dimensional (3D) volumetric ultrasound imaging and 3D focus control. Ultrasound imaging and therapeutic applications for the brain of small animals demonstrated the multi-functional capability of the dual-mode 2D matrix array. A method of rat brain positioning based on ultrasound imaging was proposed and verified. Transcranial ultrasound image-guided bloodbrain barrier (BBB) opening of multiple-targets was achieved in vivo, using the proposed dual-mode 2D array. The obtained results indicate that the dual-mode 2D matrix array is a promising method for practical use in ultrasound image-guided non-invasive therapy applications.
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http://dx.doi.org/10.1109/TBME.2021.3073951DOI Listing
April 2021

Retracted: Effects of 3-Tetrazolyl Methyl-3-Hydroxy-Oxindole Hybrid (THOH) on Cell Proliferation, Apoptosis, and G2/M Cell Cycle Arrest Occurs by Targeting Platelet-Derived Growth Factor D (PDGF-D) and the MEK/ERK Signaling Pathway in Human Lung Cell Lines SK-LU-1, A549, and A-427.

Med Sci Monit 2021 Mar 25;27:e932328. Epub 2021 Mar 25.

Department of Respiration Medicine, The People's Hospital of Henan Province, Zhengzhou, Henan, China (mainland).

An editorial decision has been made to retract this manuscript due to breach of publishing guidelines, following the identification of non-original and manipulated figures. Reference: Peng Li, Zhiqiang Zhang, Feng Zhang, Hongling Zhou, Bei Sun: Effects of 3-Tetrazolyl Methyl-3-Hydroxy-Oxindole Hybrid (THOH) on Cell Proliferation, Apoptosis, and G2/M Cell Cycle Arrest Occurs by Targeting Platelet-Derived Growth Factor D (PDGF-D) and the MEK/ERK Signaling Pathway in Human Lung Cell Lines SK-LU-1, A549, and A-427. Med Sci Monit 2018; 24: 4547-4554. 10.12659/MSM.909125.
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http://dx.doi.org/10.12659/MSM.932328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009257PMC
March 2021

Effects of microplastics and glyphosate on growth rate, morphological plasticity, photosynthesis, and oxidative stress in the aquatic species Salvinia cucullata.

Environ Pollut 2021 Jun 13;279:116900. Epub 2021 Mar 13.

Center for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing, 100084, China. Electronic address:

Glyphosate and microplastics are widely found in marine, terrestrial, and freshwater environments due to their globally widespread application. Further, they have proved to have specific ecotoxicity effects on aquatic plants. However, few studies have focused on the effects of small plastic particles and glyphosate, or especially, their combined effect on vascular plants in freshwater ecosystems. This study aimed to conduct a simulated greenhouse experiment to investigate the ecotoxicity of polystyrene microplastics and glyphosate on the floating plant Salvinia cucullata by exposure to fluorescent polystyrene microplastics (1 μm; concentration, 3, 15, and 75 mg/L), glyphosate (5, 25, and 50 mg/L), and a mixture of the two (3 + 5, 15 + 25, and 75 + 50 mg/L) for seven days. Glyphosate significantly reduced the relative growth rate, photosynthetic capacity, and root activity of S. cucullata. Polystyrene microplastics did not significantly influence photosynthesis or leaf morphological characteristics but they significantly reduced relative growth rate and root activity in S. cucullata, indicating that the effects of microplastics on aquatic plants are potentially associated with different organs exposed to pollution. Polystyrene microplastics and glyphosate activated the plant antioxidant defense systems by increasing antioxidative enzyme activities including, superoxide dismutase, ascorbate peroxidase, and catalase to cope with oxidative stress. Synergistic effects (only observed in percent leaf yellowing) were observed when S. cucullata was exposed to a high concentrations (≥15 + 25 mg/L) of glyphosate and microplastics. Our results indicate that pervasive microplastics and herbicide contamination in freshwater may potentially affect the growth of aquatic plants.
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http://dx.doi.org/10.1016/j.envpol.2021.116900DOI Listing
June 2021

Perineural Dexmedetomidine Reduces the Median Effective Concentration of Ropivacaine for Adductor Canal Block.

Med Sci Monit 2021 Mar 17;27:e929857. Epub 2021 Mar 17.

Department of Orthopedics, The First Center Hospital of Bao Ding, Baoding, Hebei, China (mainland).

BACKGROUND Multimodal analgesic regimens are well known as the best option for total knee arthroplasty. They include the adductor canal block, combined with local infiltration analgesia and a block of the interspace between the popliteal artery and the capsule of the posterior knee. However, these analgesic techniques all require a large amount of local anesthetics. In this study, we explored whether the quantity of local anesthetics could be decreased by using dexmedetomidine for the adductor canal block. MATERIAL AND METHODS Fifty-four patients scheduled for unilateral, primary total knee arthroplasty were allocated into 2 groups: the ropivacaine group (group R) and the dexmedetomidine group (group RD). Ropivacaine 0.5% was chosen as the initial concentration, and the concentration was decreased or increased according to the response of the previous participant. Based on Dixon's up-and-down method, the median effective concentration was calculated. RESULTS The quadriceps strength was similar between the 2 groups, both at 30 min after adductor canal block and during recovery from general anesthesia in the Postanesthesia Care Unit. None of the patients in this study exhibited bradycardia or hypotension. The median effective concentration of ropivacaine for adductor canal block was 0.29% (95% confidence interval [CI], 0.28-0.31%) in group RD, which was lower than that in group R (0.38% [95% CI, 0.36-0.41%]). CONCLUSIONS This study found perineural dexmedetomidine 1 μg/kg could reduce the median effective concentration of ropivacaine for the adductor canal block.
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http://dx.doi.org/10.12659/MSM.929857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983321PMC
March 2021

Linear active disturbance rejection control for the electro-hydraulic position servo system.

Sci Prog 2021 Jan-Mar;104(1):368504211000907

Tai'an Hualu Forging Machine Co. Ltd, Tai'an, P. R. China.

Valve-controlled asymmetric cylinder is widely used in servo loading system. As a kind of typical electro-hydraulic servo system (EHSS), it inherently has the characteristics such as high order nonlinear, strong coupling, and uncertain, therefore, conventional control strategy is difficult to satisfy the requirements of high-performance control. In this paper, a novel linear active disturbance rejection control (LADRC) method was proposed, in which the internal and external disturbances were actively estimated by the third-order linear extended state observer (LESO) in real-time, and rejected by the control law of proportional integral control (PID) with acceleration feed-forward. The stability of the proposed method was proved, and the influence rules of the LADRC parameters on the control performance were revealed by simulation. Finally, comparative experiments between LADRC and PID control were carried out, results showed that the disturbances can be effectively compensated and the control goals can be successfully achieved with the proposed method.
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http://dx.doi.org/10.1177/00368504211000907DOI Listing
March 2021

Motor Function Assessment of Upper Limb in Stroke Patients.

J Healthc Eng 2021 24;2021:6621950. Epub 2021 Feb 24.

School of Sports Engineering, Beijing Sport University, Beijing, China.

Background: Quantitative assessment of motor function is extremely important for poststroke patients as it can be used to develop personalized treatment strategies. This study aimed to propose an evaluation method for upper limb motor function in stroke patients.

Methods: Thirty-four stroke survivors and twenty-five age-matched healthy volunteers as the control group were recruited for this study. Inertial sensor data and surface electromyography (sEMG) signals were collected from the upper limb during voluntary upward reaching. Five features included max shoulder joint angle, peak and average speeds, torso balance calculated from inertial sensor data, and muscle synergy similarity extracted from sEMG data by the nonnegative matrix factorization algorithm. Meanwhile, the Fugl-Meyer score of each patient was graded by professional rehabilitation therapist.

Results: Statistically significant differences were observed among severe, mild-to-moderate, and control group of five features ( ≤ 0.001). The features varied as the level of upper limb motor function changes since these features significantly correlated with the Fugl-Meyer assessment scale ( ≤ 0.001). Moreover, the Bland-Altman method was conducted and showed high consistency between the evaluation method of five features and Fugl-Meyer scale. Therefore, the five features proposed in this paper can quantitatively evaluate the motor function of stroke patients which is very useful in the rehabilitation process.
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http://dx.doi.org/10.1155/2021/6621950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932780PMC
February 2021

Mechanism and regio- and stereoselectivity in an NHC-catalyzed Mannich/lactamization domino reaction.

Phys Chem Chem Phys 2021 Mar;23(10):6204-6212

School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, P. R. China.

Density functional theory (DFT) calculations (M06-2X) have been employed to disclose the mechanisms and regio- and stereo-selectivities of the N-heterocyclic carbene (NHC)-catalyzed reaction of 2-benzothiazolimines and α-chloroaldehydes. The preferred mechanism is initiated by the nucleophilic attack of NHC on α-chloroaldehyde (first step), followed by 1,2-proton transfer which was assisted by the Brønsted acid DABCO·H+ to generate the Breslow intermediate (second step). The cleavage of the C-Cl bond (third step) and deprotonation (fourth step) form the enolate intermediate. This further reacts with 2-benzothiazolimine which leads to the formation of a new C-C bond (fifth step). Subsequent cyclization takes place via the formation of a new C-N bond (sixth step). Catalyst regeneration completes the whole catalytic cycle and affords the final product (seventh step). The DFT results indicate that the fifth step determines the stereochemistry of the reaction and leads to benzothiazolopyrimidinone with the SS configuration, which agrees well with experimental observations. Intramolecular cyclization is found to be the regioselectivity-determining step, for which the [4+2] annulation pathway is more preferred than that via [2+2] annulation, which again agrees well with experimental observations. Based on the mechanism proposed, the origins of regio- and stereoselectivities have also been investigated by performing distortion/interaction, natural bond orbital (NBO) and non-covalent interaction (NCI) analyses. The mechanistic insights gained in this work should be helpful in the rational design of potential catalysts for analogous reactions.
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http://dx.doi.org/10.1039/d1cp00369kDOI Listing
March 2021

Long term follow-up of inguinal endometriosis.

BMC Womens Health 2021 03 2;21(1):90. Epub 2021 Mar 2.

Department of Obstetrics and Gynecology, Beijing Chao-Yang Hospital Affiliated To Capital Medical University, 8 Gongtinanlu, ChaoYang District, Beijing, 100020, People's Republic of China.

Background: Inguinal endometriosis (IEM) is a rare extra pelvic endometriosis. Here, we study the clinical characteristics, management strategies, and long-term gynecological outcomes of IEM patients at Beijing Chaoyang Hospital.

Case Presentation: Three patients presented with a total of four lesions (one on the left side, one on the right side, and one bilaterally). The diameters of the four lesions were 2 cm, 2 cm, 3.5 cm and 1.5 cm, respectively. Two patients were admitted with inguinal hernias. Two patients were admitted with endometrioses-one with ovarian endometriosis and one with pelvic endometriosis. The hernia sac was repaired concomitantly via excision of the round ligament in two patients. One patient underwent a concomitant laparoscopy for gynecologic evaluations, including an ablation to the peritoneal endometriosis, and resection of the left uterosacral ligament endometriosis and pelvic adhesiolysis. All lesions were located on the extraperitoneal portion of the round ligament and were diagnosed histologically. No recurrence was observed in the inguinal region. All patients diagnosed with adenomyosis were treated with medication alone without any complaints.

Conclusions: Inguinal endometriosis can occur simultaneously with pelvic endometriosis. In most cases, a concomitant hernia sac appears together with groin endometriosis. Clinical management should be individualized and performed in tandem with general practitioners and obstetrics & gynecology experts. Pelvic disease, in particular, should be followed-up by a gynecologist.
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http://dx.doi.org/10.1186/s12905-021-01235-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927249PMC
March 2021

Pyromellitic-Based Low Molecular Weight Gelators and Computational Studies of Intermolecular Interactions: A Potential Additive for Lubricant.

Langmuir 2021 Mar 26;37(9):2954-2962. Epub 2021 Feb 26.

Key Laboratory for Functional Material, Educational Department of Liaoning Province, School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, P. R. China.

Low molecular weight gelators (LMWG) have been extensively explored in many research fields due to their unique reversible gel-sol transformation. Intermolecular interactions between LMWG are known as the main driving force for self-assembly. During this self-assembly process, individually analyzing the contribution difference between various intermolecular interactions is crucial to understand the gel properties. Herein, we report 2,5-bis(hexadecylcarbamoyl)terephthalic acid () as a LMWG, which could efficiently form a stable organogel with -hexadecane, diesel, liquid paraffin, and base lubricant oil at a relatively low concentration. To investigate the contribution difference of intermolecular interactions, we first finished FT-IR spectroscopy and XRD experiments. On the basis of the -spacing, a crude simulation model was built and then subjected to molecular dynamics (MD) simulations. Then, we knocked out the energy contribution of the H-bonding interactions and π-π stacking, respectively, to evaluate the intermolecular interactions significantly influencing the stability of the gel system. MD simulations results suggest that the self-assembly of the aggregates was mainly driven by dense H-bonding interactions between carbonyl acid and amide moieties of , which is consistent with FT-IR data. Moreover, wave function analysis at a quantum level suggested these electrostatic interactions located in the middle of the molecule were surrounded by strong dispersion attraction originating from a hydrophobic environment. Furthermore, we also confirmed that 2 wt % was able to form gel lubricant with 150BS. The coefficient of friction (COF) data show that the gel lubricant has a better tribological performance than 150BS base lubricant oil. Finally, XPS was performed and offered valuable information about the lubrication mechanism during the friction.
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http://dx.doi.org/10.1021/acs.langmuir.0c03625DOI Listing
March 2021

Changes in surface characteristics and adsorption properties of 2,4,6-trichlorophenol following Fenton-like aging of biochar.

Sci Rep 2021 Feb 22;11(1):4293. Epub 2021 Feb 22.

Institute of Soil Science, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, 46300, Pakistan.

Fenton-like system formed in a natural soil environment deemed to be significant in the aging process of biochar. Aged biochars have distinct physico-chemical and surface properties compared to non-aged biochar. The aged biochar proved to be useful soil amendment due to its improved elements contents and surface properties. The biochar aging process resulted in increased surface area and pore volume, as well as carbon and oxygen-containing functional groups (such as C=O, -COOH, O-C=O etc.) on its surface, which were also associated with the adsorption behavior of 2,4,6-trichlorophenol (2,4,6-TCP). The biochar aging increased the adsorption capacity of 2,4,6-TCP, which was maximum at pH 3.0. The 2,4,6-TCP adsorption capacity of aged-bush biochar (ABB) and aged-peanut shell biochar (APB) was increased by 1.0-11.0% and 7.4-38.8%, respectively compared with bush biochar (BB) and peanut shell biochar (PB) at the same initial concentration of 2,4,6-TCP. All biochars had similar 2,4,6-TCP desorption rates ranging from 33.2 to 73.3% at different sorption temperatures and times. The desorbed components were mainly 2,4,6-TCP and other degraded components, which were low in concentration with small molecule substance. The results indicated that the aged-biochar could be effective for the long-term remediation of naturally organic polluted soils.
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http://dx.doi.org/10.1038/s41598-021-82129-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900105PMC
February 2021

Flexible Pico-Liter Acoustic Droplet Ejection Based on High-Frequency Ultrasound Transducer.

IEEE Trans Ultrason Ferroelectr Freq Control 2021 Jun 25;68(6):2212-2218. Epub 2021 May 25.

Acoustic droplet ejection (ADE) uses the acoustic energy produced by a focused ultrasound beam to provide a noncontact, highly precise, automatic, and cost-effective liquid transfer method for life science applications. The reported minimum precision of the current acoustic liquid transfer technology is 1 nL. Since precision improvement always brings valuable results in biological research, it is highly necessary to develop pico-liter precision liquid transfer technology. In this work, we developed a 40-MHz ultrahigh -frequency focused ultrasound transducer with a large aperture of 7×7 mm and a wide bandwidth of 76.4%. The designed transducer can successfully eject pico-liter droplets, and the droplet ejection accuracy ranges from 28 to 439 pL. The effects of the acoustic parameters, including excitation amplitude, pulsewidth, and frequency, on the size of the ejected droplet were studied. A wide range of ejected droplet sizes could be obtained by adjusting the acoustic parameters, thereby making liquid transfer flexible. The flexible pico-liter liquid transfer based on the wide-bandwidth, high-frequency ultrasound transducer is easier to achieve automatically, and thus it has broad prospects in biological research and industrial applications.
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http://dx.doi.org/10.1109/TUFFC.2021.3059904DOI Listing
June 2021

Systematically disrupted functional gradient of the cortical connectome in generalized epilepsy: Initial discovery and independent sample replication.

Neuroimage 2021 04 4;230:117831. Epub 2021 Feb 4.

The Clinical Hospital of Chengdu Brain Science Institute, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, P R China; MOE Key Lab for Neuroinformation, High-Field Magnetic Resonance Brain Imaging Key Laboratory of Sichuan Province, University of Electronic Science and Technology of China, Chengdu 611731, P R China. Electronic address:

Genetic generalized epilepsy is a network disorder typically involving distributed areas identified by classical neuroanatomy. However, the finer topological relationships in terms of continuous spatial arrangement between these systems are still ambiguous. Connectome gradients provide the topological representations of human macroscale hierarchy in an abstract low-dimensional space by embedding the functional connectome into a set of axes. Leveraging connectome gradients, we systematically scrutinized abnormalities of functional connectome gradient in patients with genetic generalized epilepsy with tonic-clonic seizure (GGE-GTCS, n = 78) compared to healthy controls (HC, n = 85), and further examined the reproducibility across multiple processing configurations and in an independent validation sample (patients with GGE-GTCS, n = 28; HC, n = 31). Our findings demonstrated an extended principal gradient at different spatial scales, network-level and vertex-level, in patients with GGE-GTCS. We found consistent results across processing parameters and in validation sample. The extended principal gradient revealed the excessive functional segregation between unimodal and transmodal systems associated with duration of epilepsy and age at seizure onset in patients. Furthermore, the connectivity profile of regions with abnormal principal gradients verified the disrupted functional hierarchy revealed by gradients. Together, our findings provided a novel view of functional system hierarchy alterations, which facilitated a continuous spatial arrangement of macroscale networks, to increase our understanding of the functional connectome hierarchy in generalized epilepsy.
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http://dx.doi.org/10.1016/j.neuroimage.2021.117831DOI Listing
April 2021

Successful Closed Reduction of Atlantoaxial Rotatory Fixation in Children-A Retrospective Study of 30 Patients.

Global Spine J 2021 Feb 3:2192568220984427. Epub 2021 Feb 3.

Department of Orthopedics, 145601National Children's Medical Center & Children's Hospital of Fudan University, Shanghai, China.

Study Design: Retrospective cohort study.

Objectives: To review our treatment experience and to investigate the process of this disease.

Methods: Clinical data of AARF patients, who received closed reduction, was retrospectively reviewed. Patients were divided into 2 groups according to the length of delay (Group I: 1 month ≤ delayed time < 3 months), Group II (delayed time < 1 months). The correlation between the length of delayed time and clinical recovery (CR), radiological recovery (RR), and total recovery time were measured. The atlantodental interval (ADI), lateral mass-dens interval (LDI) and lateral joint space (LJS) were compared at admission and final follow-up.

Results: 30 children (12 girls and 18 boys) with AARF had received conservative treatment. The mean age at initial treatment was 8.13-year-old, ranging from 5 to 14. The mean follow-up time was 26.93 months (range, 6-87 months). The average length of delayed time was 28.53 days (range, 2-80 days). When the LDI, LJS, and ADI differences are compared at admission and the final visit, the differences are reduced significantly on LDI and LJS. A positive correlation is observed between the length of the delay and CR time and total recovery time (r = 0.63, p = 0.00 and r = 0.47, p = 0.01) respectively.

Conclusions: Pediatric AARF patients who have a delay time < 3 months can be treated with closed reduction successfully. The longer the delayed time, the longer the traction time, but the cervical collar time is almost the same. The LDI and LJS on the anteroposterior of X-rays are convenient to estimate the progress of this condition during the treatment.
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http://dx.doi.org/10.1177/2192568220984427DOI Listing
February 2021

Nutrient sensing and cAMP signaling in yeast: G-protein coupled receptor versus transceptor activation of PKA.

Microb Cell 2020 Oct 12;8(1):17-27. Epub 2020 Oct 12.

Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, B-3001 Leuven-Heverlee, Flanders, Belgium.

A major signal transduction pathway regulating cell growth and many associated physiological properties as a function of nutrient availability in the yeast is the protein kinase A (PKA) pathway. Glucose activation of PKA is mediated by G-protein coupled receptor (GPCR) Gpr1, and secondary messenger cAMP. Other nutrients, including nitrogen, phosphate and sulfate, activate PKA in accordingly-starved cells through nutrient transceptors, but apparently without cAMP signaling. We have now used an optimized EPAC-based fluorescence resonance energy transfer (FRET) sensor to precisely monitor cAMP levels after nutrient addition. We show that GPCR-mediated glucose activation of PKA is correlated with a rapid transient increase in the cAMP level , whereas nutrient transceptor-mediated activation by nitrogen, phosphate or sulfate, is not associated with any significant increase in cAMP . We also demonstrate direct physical interaction between the Gap1 amino acid transceptor and the catalytic subunits of PKA, Tpk1, 2 and 3. In addition, we reveal a conserved consensus motif in the nutrient transceptors that is also present in Bcy1, the regulatory subunit of PKA. This suggests that nutrient transceptor activation of PKA may be mediated by direct release of bound PKA catalytic subunits, triggered by the conformational changes occurring during transport of the substrate by the transceptor. Our results support a model in which nutrient transceptors are evolutionary ancestors of GPCRs, employing a more primitive direct signaling mechanism compared to the indirect cAMP second-messenger signaling mechanism used by GPCRs for activation of PKA.
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http://dx.doi.org/10.15698/mic2021.01.740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780724PMC
October 2020

Metabolomics analysis of the effects of temperature on the growth and development of juvenile European seabass (Dicentrarchus labrax).

Sci Total Environ 2021 May 14;769:145155. Epub 2021 Jan 14.

Key Laboratory of Environment Controlled Aquaculture (KLECA), Ministry of Education, 52 Heishijiao Street, Dalian 116023, China; College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China. Electronic address:

Temperature variations have significant impacts on the growth and development of fish. In this study, the effects of temperature on the growth and development of European seabass (Dicentrarchus labrax) were investigated using ultra-performance liquid chromatography-tandem mass spectrometry-based metabolomics. Three groups of fish were exposed to various temperatures for 60 days: T1-E (10 °C), T2-E (15 °C), and T3-E (20 °C). Afterward, the temperature of all groups was increased to 20 °C and maintained for 62 days (T1-S, T2-S, T3-S). The livers were extracted for subsequent analysis. In the first stage of the experiment, the growth rate was highest in the T3-E group, followed by the T1-E and T2-E groups. The following metabolites identified by comparative analysis were found to be elevated: L-thyroxine, cysteamine, uridine diphosphate (UDP)-glucose, α-ketoglutaric acid, carbamoyl phosphate, and guanidine acetic acid of the T1-E group. Pathway analysis of the altered metabolites suggested changes in glucose metabolism, arginine and proline metabolism, the tricarboxylic acid cycle, the ornithine cycle, histidine metabolism, and taurine metabolism, which were involved with growth and development. Meanwhile, partial compensatory growth was observed in fish in the T1-S and T2-S groups. Metabolites identified as potential markers of growth included L-cysteine, taurocholic acid, UDP-glucose, and L-thyroxine. The significantly changed metabolic pathways were cysteine and methionine metabolism, bile secretion, tyrosine metabolism, and hypotaurine metabolism. We screened out the marker metabolites and metabolic pathway could provide important insights into the potential mechanisms of temperature affects the growth and development of European seabass. All in all, our research can provide theoretical basis and technical guidance for efficiently culturing European seabass.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145155DOI Listing
May 2021

Mild hypothermia protects rat cortical neurons against oxygen-glucose deprivation/reoxygenation injury via the PI3K/Akt pathway.

Neuroreport 2021 03;32(4):312-320

Department of Anesthesiology, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Ischemic stroke is the most frequent cause of long-term morbidity and mortality in the elderly worldwide. Mild hypothermia (32-35°C) has been found to have a neuroprotective effect against ischemic stroke. However, the protective mechanisms remain unclear. In the present study, we explore the neuroprotective effect of mild hypothermia in neuron-astrocyte cocultures by oxygen-glucose deprivation/reoxygenation (OGD/R) as well as the underlying mechanisms. Thionin staining was performed and cell viability, extracellular glutamate concentration and the phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) pathway-related proteins were detected after OGD/R. The results indicated that mild hypothermia significantly alleviated damage to Nissl bodies and increased the viability of neurons, which alleviated OGD/R-triggered neuronal injury. Furthermore, mild hypothermia significantly enhanced the phosphorylation of Akt (pAkt) and glutamate transporter-1 (GLT-1) and reduced extracellular glutamate concentration after OGD/R. When the PI3K inhibitor LY294002 was added, neuronal viability and the expression of pAkt and GLT-1 decreased, and extracellular glutamate concentration increased. The protective effect of mild hypothermia was counteracted by LY294002. There was no significant change in neuronal viability or the expression of pAkt and GLT-1 in the group treated with dihydrokainate, an inhibitor of GLT-1-function, compared with the mild hypothermia + OGD/R (HOGD) group, but extracellular glutamate concentration was increased. Consequently, mild hypothermia promoted glutamate clearance by regulating GLT-1 expression via the PI3K/Akt pathway, providing a neuroprotective effect against OGD/R injury.
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http://dx.doi.org/10.1097/WNR.0000000000001593DOI Listing
March 2021

A GPAT1 Mutation in Arabidopsis Enhances Plant Height but Impairs Seed Oil Biosynthesis.

Int J Mol Sci 2021 Jan 14;22(2). Epub 2021 Jan 14.

Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China.

Glycerol-3-phosphate acyltransferases (GPATs) play an important role in glycerolipid biosynthesis, and are mainly involved in oil production, flower development, and stress response. However, their roles in regulating plant height remain unreported. Here, we report that Arabidopsis GPAT1 is involved in the regulation of plant height. GUS assay and qRT-PCR analysis in Arabidopsis showed that is highly expressed in flowers, siliques, and seeds. A loss of function mutation in was shown to decrease seed yield but increase plant height through enhanced cell length. Transcriptomic and qRT-PCR data revealed that the expression levels of genes related to gibberellin (GA) biosynthesis and signaling, as well as those of cell wall organization and biogenesis, were significantly upregulated. These led to cell length elongation, and thus, an increase in plant height. Together, our data suggest that knockout of impairs glycerolipid metabolism in Arabidopsis, leading to reduced seed yield, but promotes the biosynthesis of GA, which ultimately enhances plant height. This study provides new evidence on the interplay between lipid and hormone metabolism in the regulation of plant height.
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http://dx.doi.org/10.3390/ijms22020785DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829857PMC
January 2021

Correlations of HACE1 expression with pathological stages, CT features and prognosis of hepatocellular carcinoma patients.

J BUON 2020 Nov-Dec;25(6):2570-2575

Radiology, Zibo Central Hospital, Zibo City, Shandong 255036, China.

Purpose: To explore the correlations of HECT domain and ankyrin repeat-containing E3 ubiquitin protein ligase 1 (HACE1) expression with the pathological stages, computed tomography (CT) features and prognosis of patients with hepatocellular carcinoma (HCC).

Methods: The clinical data were randomly collected from 70 patients with primary HCC. The messenger RNA (mRNA) and HACE1 in the cancer and paracancer tissues were determined via real-time quantitative-polymerase chain reaction (qRT-PCR). The curve of the relationship between HACE1 expression and patients' overall survival (OS) was plotted using the Kaplan-Meier method. Finally, the CT imaging data of patients were pooled to analyze the relationships of HACE1 expression with CT signs.

Results: Compared with those in the paracancer tissues, the mRNA and protein expression levels of HACE1 declined significantly in HCC tissues (p<0.05). It was found through analyzing the clinical indicators that the expression level of HACE1 was considerably correlated with the tumor diameter, tumor-node-metastasis (TNM) stages and pathological grades (p<0.05). The survival analysis revealed that the OS of patients in Low HACE1 group was shorter than that in High HACE1 group (median OS: 12.40 months vs. 15.16 months, p=0.031). Besides, as indicated by CT examination, the expression of HACE1 was not correlated with the number of tumors (p>0.05), but notably associated with the size, capsule and necrosis of tumors (p<0.05).

Conclusions: HCC tissues are significantly deficient in HACE1, and the combination of HACE1 and CT images may serve as an efficacious strategy for the clinical diagnosis and monitoring of HCC.
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January 2021