Publications by authors named "Zhiqiang Wang"

941 Publications

Clinical impact of new diagnostic criteria for postcapillary pulmonary hypertension.

Int J Cardiol 2021 Jul 20. Epub 2021 Jul 20.

The Second Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China. Electronic address:

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http://dx.doi.org/10.1016/j.ijcard.2021.07.038DOI Listing
July 2021

Up-regulated long noncoding RNA AC007128.1 and its genetic polymorphisms associated with Tuberculosis susceptibility.

Ann Transl Med 2021 Jun;9(12):1018

Department of Clinical Laboratory Medicine, the First Medical Center, Chinese PLA General Hospital, Beijing, China.

Background: Tuberculosis (TB) remains a major public health problem. Long non-coding RNAs (lncRNAs) are important regulators of gene expression. In this study, we explored the association between the expression of lncRNA AC007128.1 and TB susceptibility.

Methods: Three single-nucleotide polymorphisms (SNPs) (rs12333784, rs6463794, and rs720964) of lncRNA AC007128.1 were selected using the 1000 Genomes Project database and offline software Haploview V4.2, and were genotyped by a customized 2×48-Plex SNPscan™ Kit.

Results: We identified two differentially expressed lncRNA including AC007128.1 and AP001065.3 in comparisons of expression profiles between ATB LTBI, LTBI HCs, and AC700128.1 expression was specifically and significantly up-regulated in TB patients by verification of external data. Gene Ontology functional enrichment analysis and co-expression network showed up-regulated mRNA was mainly involved in negative regulation of the G protein-coupled receptor (GPCR) signaling pathway, and FPR1 and CYP27B1 were involved in the co-expression of AC007128.1. Using the 1000 Genomes Project, software Haploview V4.2, and SNP genotype, we screened out SNP rs12333784 which locus at 7p21.3 in AC007128.1 associated with TB susceptibility. The G carrier of rs12333784 was then finally verified to be significantly associated with pulmonary TB (PTB) and extrapulmonary tuberculosis (EPTB) susceptibility (pBonferroni =0.03878), and a similar but more significant effect was observed under the dominant model analysis (pBonferroni =0.013, OR =1.349, 95% CI, 1.065-1.709). In addition, the GG + GA genotype of SNP rs12333784 was significantly correlated with higher glucose (GLU) (P=0.03), higher gamma-glutamyl transferase (GGT) (P=0.05), and higher erythrocyte sedimentation rate (ESR) (P=0.05).

Conclusions: Our findings show lncRNA AC007128.1 can be regarded as biomarkers discriminating between ATB and LTBI and may also be a diagnostic biomarker for LBTI. These findings may aid clinical decision making in the management of TB.
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http://dx.doi.org/10.21037/atm-21-2724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267308PMC
June 2021

Significant Improvement of Catalytic Performance for Chlorinated Volatile Organic Compound Oxidation over RuO Supported on Acid-Etched CoO.

Environ Sci Technol 2021 Jul 16. Epub 2021 Jul 16.

Key Laboratory for Advanced Materials and Research Institute of Industrial Catalysis, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.

Ru catalysts have attracted increasing attention in catalytic oxidation of chlorinated volatile organic compounds (CVOCs). However, the development of Ru catalysts with high activity and thermal stability for CVOC oxidation still poses significant challenges due to their restrictive relationship. Herein, a strategy for constructing surface defects on CoO support by acid etching was utilized to strengthen the interaction between active RuO species and the CoO support. Consequently, both the dispersity and thermal stability of RuO species were significantly improved, achieving both high activity and stability of Ru catalysts for CVOC oxidation. The optimized Ru catalyst on the HF-etched CoO support (Ru/CoO-F) achieved complete oxidation of vinyl chloride at 260 °C under 30 000 mL·g·h, which was lower than 300 °C for the Ru catalyst on the original CoO (Ru/CoO). More importantly, the Ru species on the Ru/CoO-F catalyst were hardly lost after calcination at 500-700 °C and even reacting at 650 °C for 120 h. On this basis, the polychlorinated byproducts over the Ru/CoO-F catalyst were almost completely effaced by phosphate modification on the catalyst surface. These findings show that the method combining acid etching of the support and phosphate modification provides a strategy for the advancement of catalyst design for CVOC oxidation.
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http://dx.doi.org/10.1021/acs.est.1c02970DOI Listing
July 2021

Redox of naphthalenediimide radicals in a 3D polyimide for stable Li-ion batteries.

Chem Commun (Camb) 2021 Jul 16. Epub 2021 Jul 16.

Department of Materials Science and Engineering, Guangdong-Hong Kong-Macao Joint Laboratory for Photonic-Thermal-Electrical Energy Materials and Devices, Southern University of Science and Technology, Shenzhen 518055, Guangdong, P. R. China.

A 3D polyimide is designed as an organic cathode for Li-ion batteries. Detailed characterization and DFT simulations demonstrate that the 3D polyimide undergoes the redox of naphthalenediimide radicals and the rigidity effect of the 3D structure contributes to the stability of the radical intermediates for high-performance organic batteries.
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http://dx.doi.org/10.1039/d1cc02426dDOI Listing
July 2021

POU2F1 Promotes Cell Viability and Tumor Growth in Gastric Cancer through Transcriptional Activation of lncRNA TTC3-AS1.

J Oncol 2021 28;2021:5570088. Epub 2021 Jun 28.

Key Laboratory of Medical Imaging and Artificial Intelligence of Hunan Province, Xiangnan University, Chenzhou 423000, China.

POU domain, class 2, transcription factor 1 (POU2F1) is involved in the development of gastric cancer (GC). However, the molecular mechanism has not been fully elucidated. Here, we identified a novel lncRNA named TTC3-AS1 that was potentially regulated by POU2F1 and investigated their roles in GC progression. Bioinformatics analysis suggested that high expression of POU2F1 predicted poor prognosis in patients with GC. We further screened out an lncRNA TTC3-AS1 that may be transcriptionally activated by POU2F1 according to the JASPAR database, and POU2F1 and TTC3-AS1 were highly expressed in GC cells and tissues compared with normal controls (NCs). Function analysis revealed that both POU2F1 and TTC3-AS1 played oncogenic roles by promoting cell viability, migration, and invasion in GC. qRT-PCR analysis showed that POU2F1 improved the expression of TTC3-AS1 in GC cells, while TTC3-AS1 knockdown or overexpression had no effect on POU2F1 expression. The results of chromatin immunoprecipitation and DNA-affinity precipitation assays indicated that POU2F1 directly bound to the promoter region of TTC3-AS1 and activated its transcription. TTC3-AS1 knockdown neutralized the protumor effects of POU2F1 overexpression in GC cell lines as well as mouse models of GC, which suggested that TTC3-AS1 mediates the oncogenic function of POU2F1. In summary, POU2F1 promoted GC progression by transcriptionally activating TTC3-AS1; thus, this study provided a new perspective for the mechanism of GC progression.
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http://dx.doi.org/10.1155/2021/5570088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260299PMC
June 2021

Preclinical efficacy against acute myeloid leukaemia of SH1573, a novel mutant IDH2 inhibitor approved for clinical trials in China.

Acta Pharm Sin B 2021 Jun 9;11(6):1526-1540. Epub 2021 Mar 9.

Zhuhai Interventional Medical Center, Zhuhai Precision Medical Center, Zhuhai People's Hospital, Zhuhai Hospital Affiliated with Jinan University, Jinan University, Zhuhai 519000, China.

Acute myeloid leukaemia (AML) is the most common form of acute leukaemia in adults, with increasing incidence with age and a generally poor prognosis. Almost 20% of AML patients express mutant isocitrate dehydrogenase 2 (mIDH2), which leads to the accumulation of the carcinogenic metabolite 2-hydroxyglutarate (2-HG), resulting in poor prognosis. Thus, global institutions have been working to develop mIDH2 inhibitors. SH1573 is a novel mIDH2 inhibitor that we independently designed and synthesised. We have conducted a comprehensive study on its pharmacodynamics, pharmacokinetics and safety. First, SH1573 exhibited a strong selective inhibition of mIDH2 R140Q protein, which could effectively reduce the production of 2-HG in cell lines, serum and tumors of an animal model. It could also promote the differentiation of mutant AML cell lines and granulocytes in PDX models. Then, it was confirmed that SH1573 possessed characteristics of high bioavailability, good metabolic stability and wide tissue distribution. Finally, toxicological data showed that SH1573 had no effects on the respiratory system, cardiovascular system and nervous system, and was genetically safe. This research successfully promoted the approval of SH1573 for clinical trials (CTR20200247). All experiments demonstrated that, as a potential drug against mIDH2 R140Q acute myeloid leukaemia, SH1573 was effective and safe.
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http://dx.doi.org/10.1016/j.apsb.2021.03.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245910PMC
June 2021

Altered Gene Expression in the Testis of Infertile Patients with Nonobstructive Azoospermia.

Comput Math Methods Med 2021 9;2021:5533483. Epub 2021 Jun 9.

Gansu Provincial Maternal and Children Health Care Hospital, China.

Background: The molecular mechanism of nonobstructive azoospermia (NOA) remains unclear. The aim of this study was to identify gene expression changes in NOA patients and to explore potential biomarkers and therapeutic targets.

Methods: The gene expression profiles of GSE45885 and GSE145467 were collected from the Gene Expression Omnibus (GEO) database, and the differences between NOA and normal spermatogenesis were analyzed. Enrichment analysis was performed to explore biological functions for common differentially expressed genes (DEGs) in GSE45885 and GSE145467. Coexpression analysis of DEGs in GSE45885 was performed, and two modules with the highest correlation with NOA were screened. Key genes were then screened from the intersection genes of the two modules and common DEGs and PPI network. The expression of key genes was validated by quantitative real-time polymerase chain reaction (qRT-PCR) experiments. Finally, through miRTarBase, miRDB, and RAID, the miRNAs were predicted to regulate key genes, respectively.

Results: A total of 345 common DEGs were identified and they were mainly related to spermatogenesis, insulin signaling pathway. Coexpression analysis of DEGs in GSE45885 yielded eight modules; MEblack and MEturquoise had the highest correlation with NOA. Six genes in MEturquoise and RNF141 in MEblack were identified as key genes. qRT-PCR experiments validated the differential expression of key genes between NOA and control. Furthermore, RNF141 was regulated by the largest number of miRNAs.

Conclusion: Our findings suggest that the significant change expression of key genes may be potential markers and therapeutic targets of NOA and may have some impact on the development of NOA.
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http://dx.doi.org/10.1155/2021/5533483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211532PMC
June 2021

Strain and ligand effects in Pt-Ni alloys studied by valence-to-core X-ray emission spectroscopy.

Sci Rep 2021 Jul 1;11(1):13698. Epub 2021 Jul 1.

Department of Chemistry, University of Western Ontario, London, ON, N6A 5B7, Canada.

Experimental detection of the Pt 5d densities of states in the valence band is conducted on a series of Pt-Ni alloys by high energy resolution valence-to-core X-ray emission spectroscopy (VTC-XES) at the Pt L-edge. VTC-XES measurements reveal that the Pt d-band centroid shifts away from the Fermi level upon dilution, accompanied by concentration-dependent Pt d-band width. The competition between the strain effect and ligand effect is observed experimentally for the first time. It is found that the d-band widths in PtNi and PtNi are broader than that of Pt metal due to compressive strain which overcompensates the effect of dilution, while it is narrower in PtNi where the ligand effect dominates. VTC-XES is demonstrated to be a powerful tool to study the Pt d-band contribution to the valence band of Pt-based bimetallic. The implication for the enhanced activity of Pt-Ni catalysts in oxygen reduction reaction is discussed.
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http://dx.doi.org/10.1038/s41598-021-93068-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249455PMC
July 2021

Negative Thermal Expansion Material: Promising for Improving Electrochemical Performance and Safety of Lithium-Ion Batteries.

J Phys Chem Lett 2021 Jul 28;12(26):6134-6142. Epub 2021 Jun 28.

School of Chemical Engineering, Sichuan University, Chengdu 610065, China.

Heat and deformation are responsible for poor performance and safety of batteries, but they cannot always be avoided. To address these two issues, ZrWO, a negative thermal expansion (NTE) material, was adopted to modify LiNiCoMnO (NCM811) to decline deformation via absorption of the generated heat. The reversible capacity of NCM811 modified with 5 wt % of ZrWO can remain at 180.6 mAh/g after 100 cycles at 60 °C and 1.0 C current rate, which increases the retention ratio of NCM811 by 14.8%, while the voltage difference between main redox peaks, , strain after cycles, and heat from DSC of NCM811 are reduced about 47.8%, 81.0%, 28.2%, and 76.0%, respectively. According to various analysis results, the side reactions are also suppressed, and the enhancing mechanisms of ZrWO for NCM811 were discussed. A general strategy is developed for the management of deformation using heat to improve performance and safety of batteries.
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http://dx.doi.org/10.1021/acs.jpclett.1c01332DOI Listing
July 2021

Enhanced Electrochemical Performance and Safety of Silicon by a Negative Thermal Expansion Material of ZrWO.

ACS Appl Mater Interfaces 2021 Jul 23;13(26):30468-30478. Epub 2021 Jun 23.

School of Chemical Engineering, Sichuan University, Chengdu 610065, China.

Silicon (Si) faces big challenges in serious volume changes for applications in spite of its high theoretical capacity. Herein, a novel and facile method was proposed to decrease the volume change by simultaneously absorbing the generated heat of only Si using a negative thermal expansion (NTE) material of ZrWO. The Si modified with 2 wt % of ZrWO exhibits excellent structural integrity, electrochemical performance, and safety under various conditions, especially at elevated temperatures. Its reversible capacities can remain 1187.2 mA h g after 50 cycles and 643.8 mA h g after 100 cycles at 2 A g (∼199 and ∼190% higher than that of Si, respectively) at 25 °C. In addition, 930.6 mA h g is maintained after 50 cycles at 60 °C (∼219% higher than that of Si). As current densities increase to 2 and 4 A g, the values still remain 1389.4 and 757.5 mA h g, respectively, much higher than that of Si. Furthermore, the strain of Si is reduced by 37.2% using ZrWO at 60 °C. Various products were analyzed, and the possible enhanced mechanism was discussed using multiple techniques. These findings exhibit significant potential for the improvement of energy materials using NTE materials by combining thermal effects and volume changes as well as the improved interface behavior.
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http://dx.doi.org/10.1021/acsami.1c01088DOI Listing
July 2021

Propagation properties of phase-locked radially-polarized vector fields array in turbulent atmosphere.

Opt Express 2021 May;29(11):16833-16844

Owing to the increasing demand for information transmission, the information capacity of free-space optical communications must be increased without being significantly affected by turbulence. Herein, based on a radially-polarized vector field array, analytical formulae for three parameters are derived: average intensity, degree of polarization, and local states of polarization (SoPs). Propagation properties varying with propagation distance, strength of turbulence, beam waist, and beamlet number are investigated. In particular, the results show that the sign of local SoPs on different receiver planes is consistent with that of the source field, and that the SoPs remain constant at specific locations as the propagation distance increases; hence, the effect of turbulence on local SoPs is slight. Meanwhile, three different SoPs, i.e., linear, right-handed, and left-handed rotation polarizations, appear at corresponding locations, thereby enabling the channel capacity to be increased. This study may not only provide a theoretical basis for vector beam array propagation in a turbulent environment, but also propose a feasible solution for increasing the channel capacity and reliability to overcome challenges in a free-space link. Additionally, this study may benefit potential applications in laser lidar and remote sensing.
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http://dx.doi.org/10.1364/OE.427003DOI Listing
May 2021

Pd-promoting reduction of zinc salt to PdZn alloy catalyst for the hydrogenation of nitrothioanisole.

J Colloid Interface Sci 2021 Jun 6;602:459-468. Epub 2021 Jun 6.

Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), College of Chemistry, Nankai University, Tianjin 300071, PR China. Electronic address:

Catalytic hydrogenation of sulfur-containing substrates is an important and challenging reaction in the chemical industry. In this work, active carbon supported PdZn alloy catalyst was prepared by self-reduction method using zinc acetate as precursor without H atmosphere. During the process of self-reduction, Zn was firstly reduced to Zn at 300 °C by active carbon and reducing gas from the decompose of acetate under the promotion of metal Pd, and Zn further reacted with metal Pd to form PdZn alloy phase at 500 °C. These PdZn/AC-X catalysts showed the higher conversion and stability for the hydrogenation of 4-nitrothioanisole than the Pd/AC-600 catalyst. The excellent catalytic performance of PdZn/AC-600 catalyst can be attributed to formation of PdZn alloy, in which electron-rich Pd atoms weaken the binding ability between Pd and S and enhance the sulfur-resistance of catalyst. On the other hand, H-TPR and DFT theory calculation further indicated that the PdZn alloy phase weakens the adsorption capacity of S. Compared with the Pd/AC-600 catalyst, the PdZn alloy phase in PdZn/AC-600 catalyst has not changed and only a small amount of sulfur-containing substrates deposited on the catalyst surface after three cycles. PdZn/AC-600 catalyst exhibited improved stability in the hydrogenation of 4-nitrothioanisole and can be used three cycles with little decrease in activity.
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http://dx.doi.org/10.1016/j.jcis.2021.06.024DOI Listing
June 2021

The application value of computed tomography in combination with intraoperative noninvasive percutaneous ultrasonic localisation of subpleural pulmonary nodules/ground-glass opacity in uniportal video-assisted thoracoscopy.

Wideochir Inne Tech Maloinwazyjne 2021 Jun 27;16(2):382-389. Epub 2020 Nov 27.

Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, China.

Introduction: This study investigates the application value of preoperative noninvasive computed tomography (CT) localisation, combined with intraoperative percutaneous ultrasonic localisation, in the precise positioning and excision of subpleural pulmonary nodules/ground-glass opacity in uniportal video-assisted thoracoscopic surgery (U-VATS).

Aim: To derive the precise positioning and excision of subpleural pulmonary nodules by CT combined with intraoperative percutaneous ultrasonic localisation and to avoid the complications caused by preoperative CT-guided puncture localisation, reduce physiological and psychological stress such as anxiety, CT radiation dose, and treatment cost, and to improve the treatment satisfaction of patients.

Material And Methods: A total of 54 patients with subpleural pulmonary nodules/ground-glass opacity (SPN/GGO), who were treated in our hospital from June 2017 to January 2020, were enrolled in this study. The patients were randomly divided into a treatment group (n = 23), and the nodules were scanned by high-resolution CT and marked at the shortest distance on the surface of the body prior to surgery. These pulmonary nodules were relocated by ultrasound at the original CT positioning points in the same body position following the administration of general anaesthesia. Then, the hookwire puncture location was performed under real-time guidance. For the control group (n = 31), the subpleural pulmonary nodules were located by CT-guided puncture and embedding a hookwire prior to surgery. Pulmonary wedge resection was performed by U-VATS in each group. The subpleural nodules were confirmed by the naked eye and rapid pathological diagnosis after surgery. The difference in positioning success rate, positioning time, the incidence of complications, and patient anxiety scores for subpleural pulmonary nodules were compared and analysed between the two groups.

Results: A total of 22 cases of subpleural nodules were successfully located in the treatment group at a success rate of 95.6% (22/23). The average positioning time for CT in combination with ultrasound was 22.0 ±5.9 min. In the control group, 31 cases of subpleural pulmonary nodules were satisfactorily located at a success rate of 100% (31/31). The average positioning time of CT was 24.2 ±5.4 min. The difference in positioning success rate and positioning time was not statistically significant (p = 0.24; p = 0.15) between the two groups. The incidence of complications and SAS anxiety scores in the treatment group were lower compared with the control group. The difference was statistically significant (p = 0.002; p < 0.001).

Conclusions: Preoperative CT combined with intraoperative percutaneous real-time noninvasive ultrasonic localisation can accurately locate subpleural pulmonary nodules, with a high degree of safety and good tolerance in patients who are suitable for U-VATS.
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http://dx.doi.org/10.5114/wiitm.2020.101238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193756PMC
June 2021

Clinical Effect of the Anterior Fascia of Tarsus Tightening Combined With Lower Eyelid Blepharoplasty for Senile Entropion.

Ann Plast Surg 2021 07;87(1):12-15

From the Department of Ophthalmology, Daping Hospital, Army Medical University, Chongqing, China.

Objective: A majority of patients with senile entropion have a lower eyelid pouch. This study aimed to explore a modified method to correct the entropion and enhance ophthalmic cosmetology.

Patients And Methods: Patients with senile entropion and lower eyelid pouches, who underwent anterior fascia of tarsus tightening combined with lower eyelid pouch plastic surgery from 2018 to 2019, were enrolled in the study. The data on operation time, postoperative effect, degree of eyelid swelling after surgery, patient satisfaction, and recurrence rate were recorded.

Results: The lower eyelid entropion was well corrected in all of the 46 patients after the surgery, and the lower eyelid pouch and saggy skin were satisfactorily repaired. After 1-year follow-up, no recurrence of lower eyelid eversion and ectropion was observed. The shape of the orbital areas was natural in all the patients, the incision scar was hidden, and the patient achieved a high degree of satisfaction.

Conclusions: In patients with senile entropion and lower eyelid pouch, anterior tarsal fascia tightening combined with lower eyelid blepharoplasty could not only increase the cure rate and reduce the recurrence rate but also achieve good appearance and improve patient satisfaction.
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http://dx.doi.org/10.1097/SAP.0000000000002908DOI Listing
July 2021

Detecting Förster resonance energy transfer in living cells by conventional and spectral flow cytometry.

Cytometry A 2021 Jun 14. Epub 2021 Jun 14.

Department of Lymphoma and Myeloma, The University of Texas-MD Anderson Cancer Center, Houston, Texas, USA.

Assays based on Förster resonance energy transfer (FRET) can be used to study many processes in cell biology. Although this is most often done with microscopy for fluorescence detection, we report two ways to measure FRET in living cells by flow cytometry. Using a conventional flow cytometer and the "3-cube method" for intensity-based calculation of FRET efficiency, we measured the enzymatic activity of specific kinases in cells expressing a genetically-encoded reporter. For both AKT and protein kinase A, the method measured kinase activity in time-course, dose-response, and kinetic assays. Using the Cytek Aurora spectral flow cytometer, which applies linear unmixing to emission measured in multiple wavelength ranges, FRET from the same reporters was measured with greater single-cell precision, in real time and in the presence of other fluorophores. Results from gene-knockout studies suggested that spectral flow cytometry might enable the sorting of cells on the basis of FRET. The methods we present provide convenient and flexible options for using FRET with flow cytometry in studies of cell biology.
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http://dx.doi.org/10.1002/cyto.a.24472DOI Listing
June 2021

Early Hospital Arrival After Acute Ischemic Stroke Is Associated With Family Members' Knowledge About Stroke.

Front Neurol 2021 26;12:652321. Epub 2021 May 26.

Department of Neurology, The General Hospital of Western Theater Command, Chengdu, China.

Prehospital delay is the major factor limiting intravenous thrombolysis and mechanical thrombectomy in acute ischemic stroke (AIS). This study aimed to: (1) identify factors related to prehospital delay and (2) determine the impact of recognition and behavior of family members on patient delay. A cross-sectional, multicenter study was conducted at six teaching hospitals in China between December 1, 2018 and November 30, 2019. Patients who experienced AIS within 7 days of onset were interviewed. Of 1,782 consecutive patients (male, 57.97%; mean age, 66.3 ± 9.65 years) who had an AIS, 267 (14.98%) patients arrived within 4.5 h and 722 (40.52%) patients arrived within 6 h of stroke onset. Among patients who arrived within 4.5 h, 103 (38.6%) received thrombolysis. Age over 65 years (OR, 2.009; 95% CI, 1.014-3.982), prior stroke (OR, 3.478; 95% CI, 1.311-9.229), blurred vision (OR, 3.95; 95% CI, 1.71-9.123), and patients deciding to seek medical help (OR, 3.097; 95% CI, 1.417-6.769) were independently associated with late arrival. In contrast, sudden onset of symptoms (OR, 0.075; 95% CI, 0.028-0.196), the National Institutes of Health Stroke Scale 7-15 (OR, 0.093; 95% CI, 0.035-0.251), consciousness disturbance (OR, 0.258; 95% CI, 0.091-0.734), weakness (OR, 0.265; 95% CI, 0.09-0.784), arrival by ambulance (OR, 0.102; 95% CI, 0.049-0.211), decision time <30 min (OR, 0.008; 95% CI, 0.003-0.018), and family member understanding stroke requires early treatment (OR, 0.224; 95% CI, 0.109-0.462) were independently associated with early arrival. The prehospital delay in China lags behind Western countries. Recognition and behavior of stroke patients' family members may play a key role in early arrival.
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http://dx.doi.org/10.3389/fneur.2021.652321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187751PMC
May 2021

Enantioselective metabolism of phenylpyrazole insecticides by rat liver microsomal CYP3A1, CYP2E1 and CYP2D2.

Pestic Biochem Physiol 2021 Jul 11;176:104861. Epub 2021 May 11.

College of Resources and Environment, Anhui Agricultural University, Key Laboratory of Agri-food Safety of Anhui Province, Hefei 230036, China. Electronic address:

The stereoselective difference of chiral pesticide enantiomers is an important factor of risk evaluation and the subject has received wide attention. In the present work, enantioselective metabolism of chiral phenylpyrazole insecticides including fipronil, ethiprole and flufiprole in rat liver microsomes was investigated in vitro. The result showed remarkable enantioselectivity for fipronil and ethiprole with the EF values of 0.11-0.58. The metabolite fipronil-sulfone was formed with the degradation of fipronil. R-Ethiprole to S-ethiprole transformation was observed, but not S-ethiprole to R-ethiprole. No enantioselective metabolism was observed for flufiprole with the EF values of 0.49-0.51. The enzymatic assays showed that the inhibition ratio of R-fipronil and S-ethiprole was 1.5-2.1times that of the corresponding enantiomers on CYP2E1 and CYP2D2 activity, leading to the enantioselective metabolism. The result of the homology modeling and molecular docking further revealed that S-fipronil (-7.56 kcal mol) and R-ethiprole (-6.45 kcal mol) performed better binding with CYP2E1 and CYP2D2, respectively. The results provided useful data for the risk evaluation of chiral phenylpyrazole insecticides on ecological safety and human health.
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http://dx.doi.org/10.1016/j.pestbp.2021.104861DOI Listing
July 2021

Characterization of novel ISAba1-bounded tet(X15)-bearing composite transposon Tn6866 in Acinetobacter variabilis.

J Antimicrob Chemother 2021 Jun 10. Epub 2021 Jun 10.

Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China.

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http://dx.doi.org/10.1093/jac/dkab182DOI Listing
June 2021

Complete chloroplast genome sequence of .

Mitochondrial DNA B Resour 2021 May 31;6(7):1814-1815. Epub 2021 May 31.

CAS Key laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.

The complete chloroplast genome of was reconstructed by reference-based assembly using Illumina paired-end data. The assembled plastome is 153,234 base pairs (bp) in length, including a pair of inverted repeat regions (IRs) of 22,662 bp each, a large single-copy region (LSC) of 89,059 bp and a small single-copy region (SSC) of 19,651 bp. A total of 115 genes were predicted from the chloroplast genome, including 74 protein coding genes, 37 tRNA genes and 4 rRNA genes. The overall GC content of chloroplast genome was 37.7%. Phylogenetic analysis with several reported chloroplast genomes showed that is closely clustered with . The complete chloroplast genome of provides new insight into the evolutionary and genomic studies of Convolvulaceae.
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http://dx.doi.org/10.1080/23802359.2021.1915202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168749PMC
May 2021

Identification of a Novel Plasmid-Mediated Carbapenemase-Encoding Gene, , in Vibrio diabolicus.

Antimicrob Agents Chemother 2021 Jul 16;65(8):e0020621. Epub 2021 Jul 16.

Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, People's Republic of China.

Here, we characterized a carbapenem-resistant Vibrio diabolicus strain of shrimp origin with various experiments and bioinformatics analysis. A novel metallo-β-lactamase (MBL) gene, , that confers resistance to β-lactams, including meropenem and cephalosporins, was identified on a plasmid-borne composite transposon, IS-IS---IS, which is capable of generating a -bearing circular intermediate. IS was found to be disseminated in multidrug-resistant (MDR) pathogens, arousing the concern of further transmission of a -bearing circular intermediate to clinical Enterobacterales strains via such insertion sequences, which warrants further investigations.
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http://dx.doi.org/10.1128/AAC.00206-21DOI Listing
July 2021

C118P, a novel microtubule inhibitor with anti-angiogenic and vascular disrupting activities, exerts anti-tumor effects against hepatocellular carcinoma.

Biochem Pharmacol 2021 Aug 30;190:114641. Epub 2021 May 30.

Department of Pharmacy, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai 519000 China. Electronic address:

Hepatocellular carcinoma (HCC), a hypervascular solid tumor, is the most leading cause of cancer mortality worldwide. Microtubule binding agents targeting tumor vasculature have been investigated and employed clinically. C118P is a newly synthesized analog of CA4 with improved water solubility and extended half-life. The current studies investigated the pharmacological effects of C118P and its active metabolite C118. Here, we first confirmed by in vitro assays that C118 exerts microtubule depolymerization activity and by molecular docking revealed that it fits to the colchicine binding site of tubulin. In addition, we found that C118P and C118 altered microtubule dynamics and cytoskeleton in human umbilical vein endothelial cells. Accordingly, we observed that C118P and C118 inhibited angiogenesis and disrupted established vascular networks using tube formation assays and chick chorioallantoic membrane angiogenesis assays. In addition, our data showed that C118P and C118 exhibited board anti-proliferative effect on various cancer cells, including HCC cell lines, in MTT assays or Sulforhodamine B assays. Moreover, we found that C118P induced G2/M phase cell cycle arrest and apoptosis in HCC cell lines BEL7402 and SMMC7721 using flow cytometry analysis and immunoblotting assays. Finally, we confirmed that C118P suppressed HCC growth via targeting tumor vasculature and inducing apoptosis in the SMMC7721 xenograft mouse model. In conclusion, our studies revealed that C118P, as a potent microtubule destabilizing agent, exerts its multiple pharmacological effects against HCC by inducing cell cycle arrest and apoptosis, as well as targeting tumor vasculature. Thus, C118P might be a promising drug candidate for liver cancer treatment.
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http://dx.doi.org/10.1016/j.bcp.2021.114641DOI Listing
August 2021

Ruiz & Pav. Root Extract: A New Source of Caffeoylquinic Acids with Antioxidant and Aldose Reductase Inhibitory Activities.

Foods 2021 May 13;10(5). Epub 2021 May 13.

Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea.

Ruiz & Pav. () has long been used as a herbal medicine in Peru; however, its phytochemicals and pharmacology need to be scientifically explored. In this study, we combined the offline 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH)-/ultrafiltration-high-performance liquid chromatography (HPLC) and high-speed counter-current chromatography (HSCCC)/pH-zone-refining counter-current chromatography (pH-zone-refining CCC) to screen and separate the antioxidants and aldose reductase (AR) inhibitors from the 70% MeOH extract of which exhibited remarkable antioxidant and AR inhibitory activities. Seven compounds were initially screened as target compounds exhibiting dual antioxidant and AR inhibitory activities using DPPH-/ultrafiltration-HPLC, which guided the subsequent pH-zone-refining CCC and HSCCC separations of these target compounds, namely 3--caffeoylquinic acid, 4--caffeoylquinic acid, 5--caffeoylquinic acid, 3,4--di-caffeoylquinic acid, 3,5--di-caffeoylquinic acid, 4,5--di-caffeoylquinic acid, and 3,4,5--tri-caffeoylquinic acid. These compounds are identified for the first time in and exhibited remarkable antioxidant and AR inhibitory activities. The results demonstrate that the method established in this study can be used to efficiently screen and separate the antioxidants and AR inhibitors from natural products and, particularly, the root extract of is a new source of caffeoylquinic acids with antioxidant and AR inhibitory activities, and it can be used as a potential functional food ingredient for diabetes.
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http://dx.doi.org/10.3390/foods10051079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152971PMC
May 2021

Diverse Functions of IAA-Leucine Resistant PpILR1 Provide a Genic Basis for Auxin-Ethylene Crosstalk During Peach Fruit Ripening.

Front Plant Sci 2021 12;12:655758. Epub 2021 May 12.

Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China.

Auxin and ethylene play critical roles in the ripening of peach () fruit; however, the interaction between these two phytohormones is complex and not fully understood. Here, we isolated a peach gene, , which encodes an indole-3-acetic acid (IAA)-amino hydrolase. Functional analyses revealed that PpILR1 acts as a transcriptional activator of 1-amino cyclopropane-1-carboxylic acid synthase (), and hydrolyzes auxin substrates to release free auxin. When Cys137 was changed to Ser137, PpILR1 failed to show hydrolase activity but continued to function as a transcriptional activator of in tobacco and peach transient expression assays. Furthermore, transgenic tomato plants overexpressing exhibited ethylene- and strigolactone-related phenotypes, including premature pedicel abscission, leaf and petiole epinasty, and advanced fruit ripening, which are consistent with increased expression of genes involved in ethylene biosynthesis and fruit ripening, as well as suppression of branching and growth of internodes (related to strigolactone biosynthesis). Collectively, these results provide novel insights into the role of IAA-amino acid hydrolases in plants, and position the PpILR1 protein at the junction of auxin and ethylene pathways during peach fruit ripening. These results could have substantial implications on peach fruit cultivation and storage in the future.
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http://dx.doi.org/10.3389/fpls.2021.655758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149794PMC
May 2021

Targeted Therapeutic Strategies in the Battle Against Pathogenic Bacteria.

Front Pharmacol 2021 12;12:673239. Epub 2021 May 12.

College of Veterinary Medicine, Yangzhou University, Yangzhou, China.

The emergence and rapid spread of antibiotic resistance in pathogenic bacteria constitute a global threat for public health. Despite ongoing efforts to confront this crisis, the pace of finding new potent antimicrobials is far slower than the evolution of drug resistance. The abuse of broad-spectrum antibiotics not only accelerates the formation of resistance but also imposes a burden on the intestinal microbiota, which acts a critical role in human homeostasis. As such, innovative therapeutic strategies with precision are pressingly warranted and highly anticipated. Recently, target therapies have achieved some breakthroughs by the aid of modern technology. In this review, we provide an insightful illustration of current and future medical targeted strategies, including narrow-spectrum agents, engineered probiotics, nanotechnology, phage therapy, and CRISPR-Cas9 technology. We discuss the recent advances and potential hurdles of these strategies. Meanwhile, the possibilities to mitigate the spread of resistance in these approaches are also mentioned. Altogether, a better understanding of the advantages, disadvantages, and mechanisms of action of these targeted therapies will be conducive to broadening our horizons and optimizing the existing antibacterial approaches.
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http://dx.doi.org/10.3389/fphar.2021.673239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149751PMC
May 2021

Emergence of bla-bearing tigecycline-resistant Klebsiella aerogenes of chicken origin.

J Glob Antimicrob Resist 2021 May 26;26:66-68. Epub 2021 May 26.

College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, P.R. China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu Province, P.R. China. Electronic address:

Objectives: The aim of this study was to characterise a tigecycline-resistant bla-bearing Klebsiella aerogenes strain (HNHF1) of chicken origin.

Methods: Strain HNHF1 was characterised by phenotypic antimicrobial susceptibility testing, PCR, conjugation assays, S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), whole-genome sequencing and bioinformatics analysis.

Results: The bla gene was located on an IncHI2 plasmid (pHNHF1_NDM-9) carrying various antimicrobial resistance genes. Moreover, the genetic context ΔISAba125-bla-ble-trpF is similar to other bla-bearing genetic contexts. TA cloning experiments showed that tet(A) variants may play a partial role in high-level tigecycline resistance in HNHF1.

Conclusion: This is the first report of a tigecycline-resistant bla-bearing IncHI2 plasmid in a K. aerogenes ST4 isolate of animal origin, which poses a great threat to public health. Further comprehensive surveillance is needed.
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http://dx.doi.org/10.1016/j.jgar.2021.04.028DOI Listing
May 2021

Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties.

Clin Transl Allergy 2021 May 14;11(3):e12034. Epub 2021 May 14.

Departments of Experimental Immunology and Otorhinolaryngology Amsterdam UMC University of Amsterdam Amsterdam The Netherlands.

Background: Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties.

Methods: To obtain monoclonal and polyclonal antibodies, mice and rabbits, respectively, were immunized with recombinant Pru p 1.01 and Pru p 1.02. The Pru p 1 levels in fruits from 83 representative peach varieties was quantified by sandwich enzyme-linked immunosorbent assay (sELISA). nPru p 1 was obtained through specific monoclonal antibody affinity purification and confirmed by Western blot and mass spectrometry. The variable Pru p 1 content of selected varieties was evaluated by Western blot and the expression level of encoding genes by quantitative polymerase chain reaction.

Results: A sELISA method with monoclonal and polyclonal antibodies was built for quantifying Pru p 1 levels in peach. Pru p 1 was mainly concentrated in the peel (0.20-73.44 μg/g, fresh weight), being very low in the pulp (0.05-9.62 μg/g) and not detected in wild peach. For the 78 peach and nectarine varieties, Pru p 1 content varied widely from 0.12 to 6.45 μg/g in whole fruit. We verified that natural Pru p 1 is composed of 1.01 and 1.02 isoallergens, and the expression level and Pru p 1 band intensity in the immunoblots were in agreement with protein quantity determined by ELISA for some tested varieties. In some cases, the reduced levels of Pru p 1 did not coincide with low Pru p 3 in the same variety in whole fruit, while some ancient wild peach and nectarines contained low levels of both allergens, and late-ripening yellow flesh varieties were usually highly allergenic.

Conclusion: Pru p 1 content is generally low in peach compared to Pru p 3. Several hypoallergenic Pru p 1 and Pru p 3 varieties, "Zi Xue Tao," "Wu Yue Xian," and "May Fire," were identified, which could be useful in trials for peach allergy patients.
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http://dx.doi.org/10.1002/clt2.12034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120414PMC
May 2021

Gut microbiome alterations in high-fat-diet-fed mice are associated with antibiotic tolerance.

Nat Microbiol 2021 Jul 20;6(7):874-884. Epub 2021 May 20.

College of Veterinary Medicine, Yangzhou University, Yangzhou, China.

Antibiotic tolerance, the ability of a typically susceptible microorganism to survive extended periods of exposure to antibiotics, has a critical role in chronic and recurrent bacterial infections, and facilitates the evolution of antibiotic resistance. However, the physiological factors that contribute to the development of antibiotic tolerance, particularly in vivo, are not fully known. Despite the fact that a high-fat diet (HFD) is implicated in several human diseases, the relationship between HFD and antibiotic efficacy is still poorly understood. Here, we evaluated the efficacy of multiple clinically relevant bactericidal antibiotics in HFD-fed mice infected with methicillin-resistant Staphylococcus aureus (MRSA) or Escherichia coli. We found that HFD-fed mice had higher bacterial burdens and these bacteria displayed lower susceptibility to bactericidal antibiotic treatment compared with mice that were fed a standard diet, while microbiota-depleted standard-diet- or HFD-fed mice showed similar susceptibility. Faecal microbiota transplantation from HFD-fed mice impaired antibiotic activity in mice fed a standard diet, indicating that alteration of the gut microbiota and related metabolites in HFD-fed mice may account for the decreased antibiotic activity. 16S rRNA sequencing and metabolomics analysis of faecal samples revealed decreased microbial diversity and differential metabolite profiles in HFD-fed mice. Notably, the tryptophan metabolite indole-3-acetic acid (IAA) was significantly decreased in HFD-fed mice. Further in vitro studies showed that IAA supplementation inhibited the formation of bacterial persisters and promoted the elimination of persisters in combination with antibiotic treatment, potentially through the activation of bacterial metabolic pathways. In vivo, the combination of IAA and ciprofloxacin increased the survival rate of HFD-fed mice infected with MRSA persisters. Overall, our data reveal that a HFD has an antagonistic effect on antibiotic treatment in a mouse model, and this is associated with the alteration of the gut microbiota and IAA production.
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http://dx.doi.org/10.1038/s41564-021-00912-0DOI Listing
July 2021

Functional and structural connective disturbance of the primary and default network in patients with generalized tonic-clonic seizures.

Epilepsy Res 2021 Aug 25;174:106595. Epub 2021 Feb 25.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, PR China. Electronic address:

Objective: The present study aims to investigate the disturbance of functional and structural profiles of patients with generalized tonic-clonic seizures (GTCS).

Methods: Resting-state fMRI and diffusion tensor imaging (DTI) data was collected from fifty-six patients and sixty-two healthy controls. Degree centrality (DC) of functional connectivity was first calculated and compared between groups using a two-sample t-test. Furthermore, the regions with significant alteration of DC in patients with GTCS were used as nodes to construct the brain network. Functional connectivity (FC) network was constructed using the Person's correlation analysis and structural connectivity (SC) network was obtained using deterministic tractography technology. Gray matter volume (GMV) and cortical thickness (CT) were computed and correlated with connective profiles.

Results: The patients with GTCS showed increased DC in the primary network (PN), including bilateral precentral gyrus, supplementary motor areas (SMA), and visual cortex, and decreased DC in core regions of default mode network (DMN), bilateral anterior insular, and supramarginal gyrus. In the present study, 14 regions were identified to construct networks. In patients, the FC and SC were increased within the sensorimotor network (mainly linking with SMA) and decreased within DMN (mainly linking with the posterior cingulate cortex (PCC)). Except for the decreased FC and SC between cerebellum and SMA, patients demonstrated increased connectivity between DMN and PN. Besides, the insula demonstrated decreased FC with DMN and increased FC with PN, without significant SC alterations in patients with GTCS. Decreased GMV in bilateral thalamus and increased GMV in frontoparietal regions were found in patients. The decreased GMV of thalamus and increased GMV of SMA positively and negatively correlated with the FC between PCC and left superior frontal cortex, the FC between SMA and left precuneus respectively.

Conclusion: Hyper-connectivity within PN helps to understand the disturbance of primary functions, especially the motor abnormality in GTCS. The hypo-connectivity within DMN suggested abnormal network organization possibly related to epileptogenesis. Moreover, over-interaction between DMN and PN and unbalanced connectivity between them and insula provided potential evidence reflecting abnormal interactions between primary and high-order function systems.
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http://dx.doi.org/10.1016/j.eplepsyres.2021.106595DOI Listing
August 2021

Extracellular electron transfer influences the transport and retention of ferrihydrite nanoparticles in quartz sand coated with Shewanella oneidensis biofilm.

J Hazard Mater 2021 09 5;417:126023. Epub 2021 May 5.

Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.

Microbial biofilm has been found to impact the mobility of nanoparticles in saturated porous media by altering physicochemical properties of collector surface. However, little is known about the influence of biofilm's biological activity on nanoparticle transport and retention. Here, the transport of ferrihydrite nanoparticles (FhNPs) was studied in quartz sands coated with biofilm of Shewanella oneidensis MR-1 that is capable of reducing Fe(III) through extracellular electron transfer (EET). It was found that MR-1 biofilm coating enhanced FhNPs' deposition under different pH/ionic strength conditions and humic acid concentrations. More importantly, when the influent electron donor (glucose) concentration was increased to promote biofilm's EET activity, the breakthrough of FhNPs in biofilm-coated sands was inhibited. A lack of continuous and stable supply of electron donor, on the contrary, led to remobilization and release of the originally retained FhNPs. Column experiments with biofilm of EET-deficient MR-1 mutants (ΔomcA/ΔmtrC and ΔcymA) further indicated that the impairment of EET activity decreased the retention of FhNPs. It is proposed that the effective surface binding and adhesion of FhNPs that is required by direct EET cannot be neglected when evaluating the transport of FhNPs in sands coated with electroactive biofilm.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126023DOI Listing
September 2021

Itaconate ameliorates methicillin-resistant -induced acute lung injury through the Nrf2/ARE pathway.

Ann Transl Med 2021 Apr;9(8):712

Department of Cardiothoracic Surgery, Affiliated Hospital of Jiangnan University, Wuxi, China.

Background: Methicillin-resistant (MRSA) are a critical predisposing factor of sepsis in the clinic. As a product of human energy metabolism and immune response, itaconate can effectively reduce inflammation in the body. This research employed 4-octyl itaconate (4-OI) to illustrate that itaconate exerted anti-inflammatory effects to protect the body from acute lung injury (ALI) induced by MRSA.

Methods: HE staining and immunohistochemistry are used to evaluate the MRSA-induced ALI in mice. WB and qPCR were used to verify the effect of 4-OI on inflammation and oxidative stress caused by MRSA. Molecular docking was used to verify the binding sites of 4-OI and Keap1.

Results: We demonstrated that 4-OI treatment increased the survival ratio, attenuated the pathological damage, inhibited neutrophil infiltration, and reduced lung bacterial burden in the mouse MRSA pneumonia model. 4-OI decreased the expression of inflammatory factors by stimulating the Nrf2 and . Furthermore, 4-OI exerted its effect by promoting nuclear transport of Nrf2 . The results of molecular docking indicated that 4-OI bound to the pocket of Keap1 and exerted a stable interaction. Both Nrf2 inhibitors (ML385) and Nrf2 mice abolished the protective effect of 4-OI on MRSA-induced inflammation both and .

Conclusions: 4-OI prevents lung damage caused by MRSA bacteremia via activating Nrf2/ARE pathway.
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http://dx.doi.org/10.21037/atm-21-1448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106008PMC
April 2021
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