Publications by authors named "Zhiqiang Liu"

665 Publications

Analysis of risk factors for different subtypes of acute coronary syndrome.

J Int Med Res 2021 May;49(5):3000605211008326

Department of Comprehensive Cardiology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Province, China.

Aims: To investigate the different risk factors among different subtypes of patients with acute coronary syndrome (ACS).

Methods: A total of 296 patients who had ACS were retrospectively enrolled. Blood and echocardiographic indices were assessed within 24 hours after admission. Differences in risk factors and Gensini scores of coronary lesions among three groups were analyzed.

Results: Univariate analysis of risk factors for ACS subtypes showed that age, and levels of fasting plasma glucose, amino-terminal pro-brain natriuretic peptide, and creatine kinase isoenzyme were significantly higher in patients with non-ST-segment elevation myocardial infarction (NSTEMI) than in those with unstable angina pectoris (UAP). Logistic multivariate regression analysis showed that amino-terminal pro-brain natriuretic peptide and the left ventricular ejection fraction (LVEF) were related to ACS subtypes. The left ventricular end-diastolic diameter was an independent risk factor for UAP and ST-segment elevation myocardial infarction (STEMI) subtypes. The severity of coronary stenosis was significantly higher in NSTEMI and STEMI than in UAP. Gensini scores in the STEMI group were positively correlated with D-dimer levels (r = 0.429) and negatively correlated with the LVEF (r = -0.602).

Conclusion: Different subtypes of ACS have different risk factors. Our findings may have important guiding significance for ACS subtype risk assessment and clinical treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/03000605211008326DOI Listing
May 2021

Identification and immunoprofiling of key prognostic genes in the tumor microenvironment of hepatocellular carcinoma.

Bioengineered 2021 Dec;12(1):1555-1575

Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Tumor microenvironment (TME) is involved in the occurrence and development of hepatocellular carcinoma (HCC), and immune cells in the TME have been implicated in its progression and treatment. However, the association of genes involved in the TME with HCC prognosis remains unclear. Thus, in this study, we obtained transcriptomic and clinicopathological data of patients with HCC from The Cancer Genome Atlas to identify key genes in TME associated with HCC prognosis. Stromal and immune cell scores were calculated using the ESTIMATE method, and differentially expressed genes (DEGs) were determined. We identified 830 DEGs, which were further subjected to survival analyses and functional enrichment analysis. Next, we identified prognostic TME-associated DEGs, established a protein-protein interaction (PPI) network, and performed Cox analysis.Consequently, four key prognostic genes (, and ) associated with TME, were identified, in which and may be potential independent prognostic factors. Age, clinical stage, N stage, and risk score were also determined as significant prognostic variables. CIBERSORT was used to predict the constitution and relative content of the immune cells, wherein M0 macrophages were the most closely related to the key genes. In conclusion, , and were associated with HCC prognosis and were important for immune cell invasion into the TME. Additionally, expression may contribute toward favorable prognosis in patients with HCC. Consequently, these genes may serve as potential biomarkers and immunotherapeutic targets for HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2021.1918538DOI Listing
December 2021

A comprehensive strategy to clarify the pharmacodynamic constituents and mechanism of Wu-tou decoction based on the constituents migrating to blood and their in vivo process under pathological state.

J Ethnopharmacol 2021 Apr 28;275:114172. Epub 2021 Apr 28.

State Key Laboratory of Electroanalytical Chemistry, National Center of Mass Spectrometry in Changchun and Jilin Provincial Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 130022, Changchun, China; School of Applied Chemistry and Engineering, University of Science and Technology of China, 230026, Hefei, China. Electronic address:

Ethnopharmacological Relevance: As a traditional Chinese medicine (TCM) formula, Wu-tou decoction has been used for treating rheumatoid arthritis (RA) for more than a thousand years. Identifying pharmacodynamic constituents (PCs) of WTD and exploring their in vivo process are very meaningful for promoting the modernization of TCM. However, the pathological state might change this process.

Aim Of The Study: Hence, it is necessary and significant to compare the process in vivo of drugs both in normal and disease state and clarify their action mechanism.

Materials And Methods: Taking Wu-tou decoction (WTD) as the research object, a comprehensive strategy based on liquid chromatography coupled with mass spectrometry (LC-MS) was developed to identify PCs, clarify and compare their absorption and distribution in normal and model rats, and then explore the potential mechanism of TCM. Firstly, the PCs in WTD were identified. Then, the pharmacokinetics (PK) and tissue distribution of these ingredients were studied. Finally, the constituents with the difference between normal and model rats were selected for target network pharmacological analysis to clarify the mechanism.

Results: A total of 27 PCs of WTD were identified. The absorption and distribution of 20 PCs were successfully analyzed. In the disease state, the absorption and distribution of all these components were improved to have better treatment effects. The results of target network pharmacological analysis indicated that PTGS1, PTGS2, ABCB1, SLC6A4, CHRM2, ESR1, ESR2, CDK2, TNF and IL-6 are 10 key targets for WTD against RA. The regulatory effects of WTD on the expression of PTGS2 and TNF were further verified. Pathway enrichment analysis showed that the key mechanism of WTD against RA is to reduce inflammation and regulate the immune response.

Conclusion: These results indicated that this strategy could better understand the in vivo process and mechanism of WTD under the pathological state. Furthermore, this strategy is also appropriate for other TCM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jep.2021.114172DOI Listing
April 2021

Silencing of circ_0078607 prevents development of gastric cancer and inactivates the ERK1/2/AKT pathway through the miR-188-3p/RAP1B axis.

Anticancer Drugs 2021 Apr 23. Epub 2021 Apr 23.

Department of Oncology, Heilongjiang Province Hospital Department of Hematology, Harbin Medical University Cancer Hospital Department of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.

Aim: The aim of this study is to explore the expression and mechanism of circ_0078607 on proliferation and apoptosis of gastric cancer.

Methods: Real time PCR (RT-PCR) was performed to detect the expression of circ_0078607 in gastric cancer tumor tissues, plasma and cell lines. Cell viability was detected by cell counting Kit-8. Cell proliferation ability was assessed by cell cycle assay. The samples were analyzed by flow cytometry for the detection of apoptosis. Luciferase assay and RNA immunoprecipitation (RIP) were carried out to verify the relationship between circ_0078607 and miR-188-3p, miR-188-3p, and RAP1B. Western blot was employed to detect the protein level of RAP1B, ERK1/2 and AKT. In vivo, the effect of circ_0078607 on gastric cancer tumor growth was detected by lentivirus vector injection.

Results: Here, we found the increased level of circ_0078607 in gastric cancer tissues, gastric cancer patients plasma and cell lines. Knockdown of circ_0078607 could prevent proliferation and induce cell apoptosis in MKN-28 cells. Then we verified that circ_0078607 could interact with miR-188-3p by performed luciferase assay and RIP. Furthermore, we observed that RAP1B was a potential target of miR-188-3p. Next, we found that miR-188-3p inhibitor or overexpression of RAP1B could prevent the anti-tumor function of sh-circ_0078607. Silencing of circ_0078607 inhibited ERK1/2/AKT signal pathways via regulating miR-188-3p/RAP1B. In vivo, knockdown of circ_0078607 inhibited tumor growth.

Conclusion: Knockdown of circ_0078607 inhibits the proliferation and induces apoptosis of gastric cancer via miR-188-3p/RAP1B signal pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/CAD.0000000000001083DOI Listing
April 2021

BFAR coordinates TGFβ signaling to modulate Th9-mediated cancer immunotherapy.

J Exp Med 2021 Jul;218(7)

Chinese Academy of Sciences Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.

TGFβ is essential for the generation of anti-tumor Th9 cells; on the other hand, it causes resistance against anti-tumor immunity. Despite recent progress, the underlying mechanism reconciling the double-edged effect of TGFβ signaling in Th9-mediated cancer immunotherapy remains elusive. Here, we find that TGFβ-induced down-regulation of bifunctional apoptosis regulator (BFAR) represents the key mechanism preventing the sustained activation of TGFβ signaling and thus impairing Th9 inducibility. Mechanistically, BFAR mediates K63-linked ubiquitination of TGFβR1 at K268, which is critical to activate TGFβ signaling. Thus, BFAR deficiency or K268R knock-in mutation suppresses TGFβR1 ubiquitination and Th9 differentiation, thereby inhibiting Th9-mediated cancer immunotherapy. More interestingly, BFAR-overexpressed Th9 cells exhibit promising therapeutic efficacy to curtail tumor growth and metastasis and promote the sensitivity of anti-PD-1-mediated checkpoint immunotherapy. Thus, our findings establish BFAR as a key TGFβ-regulated gene to fine-tune TGFβ signaling that causes Th9 induction insensitivity, and they highlight the translational potential of BFAR in promoting Th9-mediated cancer immunotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1084/jem.20202144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091105PMC
July 2021

Inhibition of Syk promotes chemical reprogramming of fibroblasts via metabolic rewiring and H S production.

EMBO J 2021 Apr 28:e106771. Epub 2021 Apr 28.

The MOE Key Laboratory of Biosystems Homeostasis & Protection and Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, China.

Chemical compounds have recently been introduced as alternative and non-integrating inducers of pluripotent stem cell fate. However, chemical reprogramming is hampered by low efficiency and the molecular mechanisms remain poorly characterized. Here, we show that inhibition of spleen tyrosine kinase (Syk) by R406 significantly promotes mouse chemical reprogramming. Mechanistically, R406 alleviates Syk / calcineurin (Cn) / nuclear factor of activated T cells (NFAT) signaling-mediated suppression of glycine, serine, and threonine metabolic genes and dependent metabolites. Syk inhibition upregulates glycine level and downstream transsulfuration cysteine biosynthesis, promoting cysteine metabolism and cellular hydrogen sulfide (H S) production. This metabolic rewiring decreased oxidative phosphorylation and ROS levels, enhancing chemical reprogramming. In sum, our study identifies Syk-Cn-NFAT signaling axis as a new barrier of chemical reprogramming and suggests metabolic rewiring and redox homeostasis as important opportunities for controlling cell fates.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.15252/embj.2020106771DOI Listing
April 2021

Gating control effect facilitates excellent gas selectivity in a novel Na-SSZ-27 zeolite.

Chem Commun (Camb) 2021 Apr;57(34):4170-4173

State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, P. R. China.

A novel Na-SSZ-27 zeolite was demonstrated to possess excellent H2/CO2 diffusion selectivity of more than 100. This investigation highlights the crucial effect of the "gating control" of the 8-ring windows on the separation, where sodium cations act as gates to selectively control the diffusion of CO2 and promote the selectivity for H2.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cc00164gDOI Listing
April 2021

Inhibition of the transient receptor potential vanilloid 3 channel attenuates carbon tetrachloride-induced hepatic fibrosis.

Biochem Biophys Res Commun 2021 Apr 24;558:86-93. Epub 2021 Apr 24.

Hunan Provincial Key Laboratory of Hepatobiliary Disease Research, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China; Department of General Surgery. Pinghu Hospital, Health Science Center, Shenzhen University, Shenzhen, 518116, China. Electronic address:

Transient receptor potential vanilloid 3 (TRPV3) is a member of the TRP superfamily. Previous studies have demonstrated that TRPV3 is associated with myocardial fibrosis. However, the role of TRPV3 in hepatic fibrosis and its underlying mechanisms are still unclear. This study aimed to elucidate the underlying effects of TRPV3 on hepatic fibrosis at multiple biological levels. First, immunohistochemical staining was performed to examine TRPV3 expression in human hepatic cirrhosis tissues. Then, we established a CCl-induced hepatic fibrosis mouse model. The TRPV3 selective agonist drofenine and its inhibitor, forsythoside B, were intraperitoneally injected to investigate the relationship between TRPV3 and liver fibrosis progression. Finally, in vitro studies were performed using hepatic stellate cells (HSCs) to discover the potential molecular biological mechanisms. Immunohistochemistry revealed TRPV3 overexpression in liver cirrhosis. In the liver fibrosis groups, TRPV3 inhibitor treatment significantly reduced liver fibrosis, while TRPV3 agonist exacerbated its progression. In HSCs, knocking down TRPV3 with siRNA impaired DNA synthesis and cell proliferation and increased cell apoptosis. Furthermore, we found that knockdown of TRPV3 could reduce the lectin like oxidized lowdensity lipoprotein receptor-1 (LOX-1) protein levels. Our research suggests that lower expression or functional levels of TRPV3 can ameliorate the inflammatory response and fibrotic tissue proliferation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrc.2021.04.065DOI Listing
April 2021

Single Exposure to Cocaine Impairs Reinforcement Learning by Potentiating the Activity of Neurons in the Direct Striatal Pathway in Mice.

Neurosci Bull 2021 Apr 27. Epub 2021 Apr 27.

Key Laboratory of Modern Teaching Technology, Ministry of Education, Shaanxi Normal University, Xi'an, 710062, China.

Plasticity in the glutamatergic synapses on striatal medium spiny neurons (MSNs) is not only essential for behavioral adaptation but also extremely vulnerable to drugs of abuse. Modulation on these synapses by even a single exposure to an addictive drug may interfere with the plasticity required by behavioral learning and thus produce impairment. In the present work, we found that the negative reinforcement learning, escaping mild foot-shocks by correct nose-poking, was impaired by a single in vivo exposure to 20 mg/kg cocaine 24 h before the learning in mice. Either a single exposure to cocaine or reinforcement learning potentiates the glutamatergic synapses on MSNs expressing the striatal dopamine 1 (D1) receptor (D1-MSNs). However, 24 h after the cocaine exposure, the potentiation required for reinforcement learning was disrupted. Specific manipulation of the activity of striatal D1-MSNs in D1-cre mice demonstrated that activation of these MSNs impaired reinforcement learning in normal D1-cre mice, but inhibition of these neurons reversed the reinforcement learning impairment induced by cocaine. The results suggest that cocaine potentiates the activity of direct pathway neurons in the dorsomedial striatum and this potentiation might disrupt the potentiation produced during and required for reinforcement learning.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12264-021-00687-8DOI Listing
April 2021

Transition Metal Catalyst-Free, Base-Promoted 1,2-Additions of Polyfluorophenylboronates to Aldehydes and Ketones.

Angew Chem Int Ed Engl 2021 Apr 26. Epub 2021 Apr 26.

Julius-Maximilians-Universität Würzburg, Institut für Anorganische Chemie, Am Hubland, 97074, Würzburg, GERMANY.

A novel protocol for the transition metal-free 1,2-addition of polyfluoroaryl boronate esters to aldehydes and ketones is reported, which provides secondary alcohols, tertiary alcohols, and ketones. Control experiments and DFT calculations indicate that both the ortho-F substituents on the polyfluorophenyl boronates and the counterion K+ in the carbonate base are critical. The distinguishing features of this procedure include the employment of commercially available starting materials and the broad scope of the reaction with a wide variety of carbonyl compounds giving moderate to excellent yields. Intriguing structural features involving O-H···O and O-H···N hydrogen bonding, as well as arene-perfluoroarene interactions, in this series of racemic polyfluoroaryl carbinols have also been addressed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202103686DOI Listing
April 2021

Highly efficient generation of bacterial leaf blight-resistant and transgene-free rice using a genome editing and multiplexed selection system.

BMC Plant Biol 2021 Apr 24;21(1):197. Epub 2021 Apr 24.

Syngenta Biotechnology (China) Co., Ltd, No.25, Life Science Park Road, Beijing, 102206, China.

Background: Rice leaf blight, which is a devastating disease worldwide, is caused by the bacterium Xanthomonas oryzae pv. oryzae (Xoo). The upregulated by transcription activator-like 1 (UPT) effector box in the promoter region of the rice Xa13 gene plays a key role in Xoo pathogenicity. Mutation of a key bacterial protein-binding site in the UPT box of Xa13 to abolish PXO99-induced Xa13 expression is a way to improve rice resistance to bacteria. Highly efficient generation and selection of transgene-free edited plants are helpful to shorten and simplify the gene editing-based breeding process. Selective elimination of transgenic pollen of T0 plants can enrich the proportion of T1 transgene-free offspring, and expression of a color marker gene in seeds makes the selection of T2 plants very convenient and efficient. In this study, a genome editing and multiplexed selection system was used to generate bacterial leaf blight-resistant and transgene-free rice plants.

Results: We introduced site-specific mutations into the UPT box using CRISPR/Cas12a technology to hamper with transcription-activator-like effector (TAL) protein binding and gene activation and generated genome-edited rice with improved bacterial blight resistance. Transgenic pollen of T0 plants was eliminated by pollen-specific expression of the α-amylase gene Zmaa1, and the proportion of transgene-free plants increased from 25 to 50% among single T-DNA insertion events in the T1 generation. Transgenic seeds were visually identified and discarded by specific aleuronic expression of DsRed, which reduced the cost by 50% and led to up to 98.64% accuracy for the selection of transgene-free edited plants.

Conclusion: We demonstrated that core nucleotide deletion in the UPT box of the Xa13 promoter conferred resistance to rice blight, and selection of transgene-free plants was boosted by introducing multiplexed selection. The combination of genome editing and transgene-free selection is an efficient strategy to accelerate functional genomic research and plant breeding.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12870-021-02979-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066475PMC
April 2021

Rickettsiae in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks in northwestern China.

Parasit Vectors 2021 Apr 19;14(1):204. Epub 2021 Apr 19.

Department of Basic Medicine, School of Medicine, Shihezi University, Shihezi, 832003, Xinjiang, Uygur Autonomous Region, China.

Background: Previously, twelve Rickettsia species were identified in ticks, fleas, sheep keds (Melophagus ovinus), bats (Pipistrellus pipistrellus) and a tick-bitten patient in the Xinjiang Uygur Autonomous Region (XUAR) in northwestern China. Here we aimed to molecularly detect rickettsial agents in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks.

Methods: During 2018-2019, 12 red foxes, one marbled polecat and their ticks were sampled in two counties and a city of the XUAR. The heart, liver, spleen, lung and kidney of these 13 carnivores were dissected, followed by DNA extraction. Hard ticks were identified both morphologically and molecularly. All samples were examined for the presence of rickettsiae by amplifying four genetic markers (17-kDa, gltA, ompA, sca1).

Results: A total of 26 adult ticks and 28 nymphs (38 Ixodes canisuga, nine Ixodes kaiseri, six Haemaphysalis erinacei and one Dermacentor marginatus) were collected from red foxes, and four Ha. erinacei ticks were removed from the marbled polecat. Analysis of cytochrome c oxidase subunit I (COI) gene sequences indicated that 2-32 nucleotides differed between I. canisuga, I. kaiseri and Ha. erinacei from northwestern China and Europe. Rickettsia raoultii was detected in three red foxes, Candidatus Rickettsia barbariae in a red fox, Rickettsia sibirica in a red fox and a marbled polecat, and R. raoultii in two tick species (I. canisuga and D. marginatus).

Conclusions: To the best of our knowledge, I. canisuga and I. kaiseri have not been previously reported from red foxes in China. The DNA of R. sibirica and R. raoultii was detected for the first time in the organs of red foxes, and R. sibirica in the organs of a marbled polecat. This is also the first molecular evidence for the presence of R. raoultii in I. canisuga. Our findings expand the range of tick-borne pathogens in wildlife species and associated ticks in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13071-021-04718-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054388PMC
April 2021

Using Deep Learning in a Monocentric Study to Characterize Maternal Immune Environment for Predicting Pregnancy Outcomes in the Recurrent Reproductive Failure Patients.

Front Immunol 2021 1;12:642167. Epub 2021 Apr 1.

Department of Pediatric and Adolescent Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.

Recurrent reproductive failure (RRF), such as recurrent pregnancy loss and repeated implantation failure, is characterized by complex etiologies and particularly associated with diverse maternal factors. It is currently believed that RRF is closely associated with the maternal environment, which is, in turn, affected by complex immune factors. Without the use of automated tools, it is often difficult to assess the interaction and synergistic effects of the various immune factors on the pregnancy outcome. As a result, the application of Artificial Intelligence (A.I.) has been explored in the field of assisted reproductive technology (ART). In this study, we reviewed studies on the use of A.I. to develop prediction models for pregnancy outcomes of patients who underwent ART treatment. A limited amount of models based on genetic markers or common indices have been established for prediction of pregnancy outcome of patients with RRF. In this study, we applied A.I. to analyze the medical information of patients with RRF, including immune indicators. The entire clinical samples set (561 samples) was divided into two sets: 90% of the set was used for training and 10% for testing. Different data panels were established to predict pregnancy outcomes at four different gestational nodes, including biochemical pregnancy, clinical pregnancy, ongoing pregnancy, and live birth, respectively. The prediction models of pregnancy outcomes were established using sparse coding, based on six data panels: basic patient characteristics, hormone levels, autoantibodies, peripheral immunology, endometrial immunology, and embryo parameters. The six data panels covered 64 variables. In terms of biochemical pregnancy prediction, the area under curve (AUC) using the endometrial immunology panel was the largest (AUC = 0.766, accuracy: 73.0%). The AUC using the autoantibodies panel was the largest in predicting clinical pregnancy (AUC = 0.688, accuracy: 78.4%), ongoing pregnancy (AUC = 0.802, accuracy: 75.0%), and live birth (AUC = 0.909, accuracy: 89.7%). Combining the data panels did not significantly enhance the effect on prediction of all the four pregnancy outcomes. These results give us a new insight on reproductive immunology and establish the basis for assisting clinicians to plan more precise and personalized diagnosis and treatment for patients with RRF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.642167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047052PMC
April 2021

Single-handed Double Helix and Spiral Platelet Formed by Racemate of Dissymmetric Cages.

Angew Chem Int Ed Engl 2021 Apr 16. Epub 2021 Apr 16.

Shanghai Jiao Tong University, School of Chemistry and Chemical Engineering, CHINA.

Since Pasteur's seminal work, spontaneous deracemization or symmetry breaking has been applied to separate homochiral domains from the erstwhile racemic system. However, the resulting homochirality can only be referred to when the scales of these domains and systems are specified. To clarify this notion, we report on the self-assembly of racemates of dissymmetric cages DC-1 with a cone-shape propeller geometry, forming a centrosymmetric columnar crystalline phase, namely racemic at crystallographic level . Due to their anisotropic geometry, the two enantiomers are packed in a frustrated fashion within this crystalline phase, in which single-handed double helices are observed, i.e. , single-handedness at supramolecular level . The frustrated packing, or layer continuity break-up, in turn facilitates screw dislocation during the crystal growth, forming left- or right-handed spiral platelets, namely symmetry-breaking at morphological level , even though each platelet is composed of DC-1 racemates. We also demonstrated the symmetry correlation between DC-1 molecules, the corresponding crystalline phase and spiral platelets, all exhibiting a C 3 -symmetry. This work might provide new insight into the relationship between racemization, symmetry-breaking and homochirality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202103821DOI Listing
April 2021

Surface Fingerprinting of Faceted Metal Oxides and Porous Zeolite Catalysts by Probe-Assisted Solid-State NMR Approaches.

Acc Chem Res 2021 Apr 15. Epub 2021 Apr 15.

State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, P. R. China.

ConspectusAcid catalysis in heterogeneous systems such as metal oxides and porous zeolites has been widely involved in various catalytic processes for chemical and petrochemical industries. In acid-catalyzed reactions, the performance (e.g., activity and selectivity) is closely associated with the acidic features of the catalysts, viz., type (Lewis vs Brønsted acidity), distribution (external vs internal surface), strength (strong vs weak), concentration (amount), and spatial interactions of acidic sites. The characterization of local structure and acidic properties of these active sites has important implications for understanding the reaction mechanism and the practical catalytic applications of acidic catalysts. Among diverse acidity characterization approaches, the solid-state nuclear magnetic resonance (SSNMR) technique with suitable probe molecules has been recognized as a reliable and versatile tool. Such a probe-assisted SSNMR approach could provide qualitative (type, distribution, and spatial interactions) and quantitative (strength and concentration) information on each acidic site. This Account aims to integrate our recent important findings in determining the structures and acidic characteristics of some typical metal oxide and zeolite catalysts by using the probe-assisted SSNMR technique, as well as clarifying the continuously evolving process of each discrete acidic site under hydrothermal or chemical treatments even at the molecular level with multiscale theoretical simulations.More specifically, we will describe herein the development and applications of the probe-assisted SSNMR methods, such as trimethylphosphine (TMP) and acetonitrile- (CDCN) in conjunction with advanced two-dimensional (2D) homo- and heteronuclear correlation spectroscopy, for characterizing the structures and properties of acidic sites in varied solid catalysts. Moreover, relevant information regarding the surface fingerprinting of various facets on crystalline metal oxide nanoparticles and active centers inside porous zeolites, the mapping of relevant spatial interactions, and the verification of structure-activity correlation were investigated as well. Relevant discussions are mainly based on the recent NMR experiments of our collaborating research groups, including (i) determining the acidic characterization with probe-assisted SSNMR approaches, (ii) mapping various active centers (or crystalline facets), and (iii) revealing their influence on catalytic performance of solid acid catalyst systems. It is anticipated that this information may provide more in-depth insights toward our fundamental understanding of solid acid catalysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.accounts.1c00069DOI Listing
April 2021

The co-occurrence of leukemoid reaction and hypercalcemia in a patient with endometrial cancer: A case report and literature review.

Sci Prog 2021 Apr-Jun;104(2):368504211004028

Shanghai First Maternity and Infant Hospital, Tongji University, School of Medicine, Pudong, Shanghai, China.

Paraneoplastic syndromes are rarely seen in gynecological tumors especially in endometrial cancer. Early identification of paraneoplastic syndromes plays a significant role in the treatment and prognosis of cancer. Here, we reported a rare case with endometrial cancer with a 2.7 cm × 2.2 cm × 3.4 cm lesion in the posterior cervix presenting leukemoid reaction and hypercalcemia as paraneoplastic syndromes simultaneously. During the progress of the endometrial cancer, her leukocyte level rose up to 60.7 × 10/L after anti-infection treatment. Meanwhile, the patient represented a series of severe clinical situation including hypercalcemia, hypokalemia, metabolic alkalosis. and respiratory failure. Finally, the patient died of respiratory circulatory failure 2 weeks later. In addition to symptomatic treatment, possible treatment targeted on the primary tumor as early as possible might help to improve the clinical prognosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/00368504211004028DOI Listing
April 2021

Molecular survey of Babesia spp. in red foxes (Vulpes Vulpes), Asian badgers (Meles leucurus) and their ticks in China.

Ticks Tick Borne Dis 2021 Mar 17;12(4):101710. Epub 2021 Mar 17.

School of Animal Science and Technology, Shihezi University, Shihezi City, Xinjiang Uygur Autonomous Region, 832000, People's Republic of China. Electronic address:

Babesia species (Apicomplexa: Piroplasmorida) are tick-borne protozoan hemoparasites, which pose a significant threat to domestic animals, wildlife and humans. This study aimed to determine and characterize Babesia species in red foxes (Vulpes vulpes), Asian badgers (Meles leucurus) and their ticks. Blood, heart, liver, spleen, lung, kidney, large intestine and small intestine were collected from 19 wild carnivores (12 red foxes and 7 Asian badgers). All ticks were removed from these animals and identified according to morphological and molecular characteristics. The samples were tested for the presence of Babesia species using the 18S rRNA gene. Molecular analyses showed that the DNA of Babesia vogeli and Babesia vulpes was present in red fox organs/tissues and blood samples. A total of 54 hard ticks (38 Ixodes canisuga, 6 Haemaphysalis erinacei, 9 Ixodes kaiseri and 1 Dermacentor marginatus) were collected from red foxes and 12 (I. kaiseri) from Asian badgers. All ticks were adults. Among them, one I. kaiseri parasiting a red fox contained the DNA of B. vulpes while one I. canisuga was positive for Babesia sp. belonging to the clade "Babesia sensu stricto". Molecular and phylogenetic analyses indicated the presence of a novel genotype, Babesia sp. "badger China". Babesia sp. badger type A and type B from Asian badgers were different from those in European badgers. Co-infection with three Babesia genotypes was found in one Asian badger. This study provides the first data on Babesia infection in red foxes, Asian badgers and their ticks in China. Babesia vogeli was detected for the first time in red foxes in Asia. Co-infection and genetic diversity of Babesia genotypes in Asian badgers were also demonstrated.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ttbdis.2021.101710DOI Listing
March 2021

An efficient deep convolutional neural network model for visual localization and automatic diagnosis of thyroid nodules on ultrasound images.

Quant Imaging Med Surg 2021 Apr;11(4):1368-1380

Department of Diagnostic and Therapeutic Ultrasonography, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Background: The aim of this study was to construct a deep convolutional neural network (CNN) model for localization and diagnosis of thyroid nodules on ultrasound and evaluate its diagnostic performance.

Methods: We developed and trained a deep CNN model called the Brief Efficient Thyroid Network (BETNET) using 16,401 ultrasound images. According to the parameters of the model, we developed a computer-aided diagnosis (CAD) system to localize and differentiate thyroid nodules. The validation dataset (1,000 images) was used to compare the diagnostic performance of the model using three state-of-the-art algorithms. We used an internal test set (300 images) to evaluate the BETNET model by comparing it with diagnoses from five radiologists with varying degrees of experience in thyroid nodule diagnosis. Lastly, we demonstrated the general applicability of our artificial intelligence (AI) system for diagnosing thyroid cancer in an external test set (1,032 images).

Results: The BETNET model accurately detected thyroid nodules in visualization experiments. The model demonstrated higher values for area under the receiver operating characteristic (AUC-ROC) curve [0.983, 95% confidence interval (CI): 0.973-0.990], sensitivity (99.19%), accuracy (98.30%), and Youden index (0.9663) than the three state-of-the-art algorithms (P<0.05). In the internal test dataset, the diagnostic accuracy of the BETNET model was 91.33%, which was markedly higher than the accuracy of one experienced (85.67%) and two less experienced radiologists (77.67% and 69.33%). The area under the ROC curve of the BETNET model (0.951) was similar to that of the two highly skilled radiologists (0.940 and 0.953) and significantly higher than that of one experienced and two less experienced radiologists (P<0.01). The kappa coefficient of the BETNET model and the pathology results showed good agreement (0.769). In addition, the BETNET model achieved an excellent diagnostic performance (AUC =0.970, 95% CI: 0.958-0.980) when applied to ultrasound images from another independent hospital.

Conclusions: We developed a deep learning model which could accurately locate and automatically diagnose thyroid nodules on ultrasound images. The BETNET model exhibited better diagnostic performance than three state-of-the-art algorithms, which in turn performed similarly in diagnosis as the experienced radiologists. The BETNET model has the potential to be applied to ultrasound images from other hospitals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/qims-20-538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930675PMC
April 2021

The effects and mechanisms of aloe-emodin on reversing adriamycin-induced resistance of MCF-7/ADR cells.

Phytother Res 2021 Mar 31. Epub 2021 Mar 31.

National Center of Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.

Multidrug resistance (MDR) is one of the major obstacles for clinical effective chemotherapy. In this study, the effects and possible mechanisms of aloe-emodin (AE) were investigated on reversing the adriamycin (ADR)-induced resistance of MCF-7/ADR cells. AE could significantly reverse the ADR resistance in MCF-7/ADR cells. The combination of AE (20 μM) and ADR had no effect on the P-glycoprotein (P-gp) level, but notably promoted the accumulation of ADR in drug-resistant cells. The efflux function of P-gp required ATP, but AE reduced the intracellular ATP level. AE played a reversal role might through inhibiting the efflux function of P-gp. The research result of energy metabolism pathways indicated that combination of AE and ADR could inhibit glycolysis, tricarboxylic acid (TCA) cycle, glutamine metabolism, and related amino acid synthesis pathways. Moreover, we found AE not only reversed ADR-induced resistant but also induced autophagy as a defense mechanism. In addition, the combination of AE and ADR arrested G2/M cell cycle and induced apoptosis through DNA damage, ROS generation, caspase-3 activation. Our study indicated that AE could be a potential reversal agent to resensitize ADR resistant in tumor chemotherapy and inhibiting autophagy might be an effective strategy to further enhance the reversal activity of AE.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ptr.7096DOI Listing
March 2021

Graphene-Nanorod Enhanced Quasi-Van Der Waals Epitaxy for High Indium Composition Nitride Films.

Small 2021 Mar 31:e2100098. Epub 2021 Mar 31.

Research and Development Center for Semiconductor Lighting Technology, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, 100083, China.

The nitride films with high indium (In) composition play a crucial role in the fabrication of In-rich InGaN-based optoelectronic devices. However, a major limitation is In incorporation requiring a low temperature during growth at the expense of nitride dissociation. Here, to overcome this limitation, a strain-modulated growth method, namely the graphene (Gr)-nanorod (NR) enhanced quasi-van der Waals epitaxy, is proposed to increase the In composition in InGaN alloy. The lattice transparency of Gr enables constraint of in-plane orientation of nitride film and epitaxial relationships at the heterointerface. The Gr interlayer together with NRs buffer layer substantially reduces the stress of the GaN film by 74.4%, from 0.9 to 0.23 GPa, and thus increases the In incorporation by 30.7%. The first principles calculations confirm that the release of strain accounts for the dramatic improvement. The photoluminescence peak of multiple quantum wells shifts from 461 to 497 nm and the functionally small-sized cyan light-emitting diodes of 7 × 9 mil are demonstrated. These findings provide an efficient approach for the growth of In-rich InGaN film and extend the applications of nitrides in advanced optoelectronic, photovoltaic, and thermoelectric devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202100098DOI Listing
March 2021

HS Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis.

Front Mol Biosci 2021 11;8:635470. Epub 2021 Mar 11.

School of Life Science, Shanxi University, Taiyuan, China.

Hydrogen sulfide (HS) is a gasotransmitter along with nitric oxide and carbon oxide, which is involved in plant growth and development as well as biotic and abiotic stress resistance. In a previous study, we reported that mitogen-activated protein kinases, especially MPK4, are important downstream components of HS involved in alleviating cold stress; however the underlying mechanism is unclear. In this study, we determined that the ability of HS to alleviate cold stress is impaired in mutants, but not in the upstream and mutants. MPK4 was basically persulfidated, and NaHS (HS donor) further increased the persulfidation level of MPK4. MEK2 was not persulfidated by HS. NaHS treatments increased the MPK4 activity level nearly tenfold. The persulfidation signal of MPK4 did not disappear after eight cystein residues in MPK4 were site-mutated, respectively. Above all, our results suggested that HS alleviates cold stress directly by persulfidating MPK4 and increasing the MPK4 kinase activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmolb.2021.635470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991836PMC
March 2021

Bim's Effect on the Expression of miR-423-3p in Promoting Primary Hepatic Cancer (PHC) and Role of miR-423-3p in PHC Proliferation and Invasion.

Biochem Genet 2021 Mar 26. Epub 2021 Mar 26.

Department of Hepatopancreatobiliary Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.

In this study, we analyzed the miR-423-3p expression in primary hepatic cancer (PHC), its effect on cell proliferation, and migration and explored Bcl-2-interacting mediator effect on the role of miR-423-3p in promoting liver cancer. The miR-423-3p expression levels in LC tissues and adjacent non-tumor tissues were compared, and the relationship between miR-423-3p and clinical pathological characteristics of patients was analyzed. These levels in peripheral blood of LC patients and healthy volunteers were compared, and the diagnostic value of miR-423-3p in LC was analyzed. The miR-423-3p and BCL-2-interacting mediators of cell death (Bim) expression in LC cells SMMC-7721 and Huh-7 were analyzed. The changes of cell proliferation, invasion, and apoptosis level were evaluated. Furthermore, the association and regulatory relationship between miR-423-3p and Bim were evaluated by dual luciferase report. The miR-423-3p expression level in LC increased, indicating miR-423-3p could be a diagnostic marker for LC. miR-423-3p expression was relatively low in patients with low TNM stage (I-II) and LC with serum AFP level ≤ 20 μg/L, related to the 5-year survival rate of LC patients. The 5-year survival rate of patients with low miR-423-3p expression was dramatically higher than that of those with high miR-423-3p expression. The miR-423-3p can promote proliferation and migration of LC cells and inhibit apoptosis, Bim can inhibit their growth and metastasis, and miR-423-3p can also regulate Bim expression. The miR-423-3p expression level in LC increased and could inhibit Bim to promote the proliferation and invasion of LC cells and inhibit apoptosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10528-021-10052-zDOI Listing
March 2021

Boronate Affinity-Based Oriented and Double-Shelled Surface Molecularly Imprinted Polymers on 96-Well Microplates for a High-Throughput Pharmacokinetic Study of Rutin and Its Metabolites.

J Agric Food Chem 2021 Apr 23;69(13):3972-3981. Epub 2021 Mar 23.

Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.

The boronate affinity-based oriented and double-shelled surface molecularly imprinted polymers on 96-well microplates (BDMIPs) were designed and applied to high-specific and high-throughput pharmacokinetic (PK) study of rutin and its metabolites from rat plasma without concentration and redissolution. It integrated the advantages of covalent effects-based boronate affinity, noncovalent effects of ethylene imine polymer (PEI) dendrimer, multiple cavities-based double-shelled layers, and multiparallel wells-based 96-well microplates. Furthermore, ultrahigh-performance liquid chromatography triple quadrupole tandem mass spectrometry (UHPLC-MS/MS) was used to accurately quantify targets. It showed lower limits of detection (LODs) up to 100-fold than the conventional method. And PKs of rutin and trace isoquercetin (IQC) were first reported at the same time. The platform can provide a fast, simple, low-cost, high-selective, high-effective, and high-throughput methodological reference for analysis of large-scale samples in the fields of agriculture and food.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.0c07431DOI Listing
April 2021

Overexpression of Annexin A2 promotes proliferation by forming a Glypican 1/c-Myc positive feedback loop: prognostic significance in human glioma.

Cell Death Dis 2021 Mar 12;12(3):261. Epub 2021 Mar 12.

Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha, China.

In order to set up a reliable prediction system for the tumor grade and prognosis in glioma patients, we clarify the complicated crosstalk of Annexin A2 (ANXA2) with Glypican 1 (GPC1) and demonstrate whether combined indexes of ANXA2 and GPC1 could improve the prognostic evaluation for glioma patients. We found that ANXA2-induced glioma cell proliferation in a c-Myc-dependent manner. ANXA2 increased the expression of GPC1 via c-Myc and the upregulated GPC1 further promoted the c-Myc level, forming a positive feedback loop, which eventually led to enhanced proliferation of glioma cells. Both mRNA and protein levels of ANXA2 were upregulated in glioma tissues and coincided with the overexpression of GPC1. Besides, we utilized tissue microarrays (TMAs) and immunohistochemistry to demonstrate that glioma patients with both high expression of ANXA2 and GPC1 tended to have higher rate of tumor recurrence and shorter overall survival (OS). In conclusion, the overexpression of ANXA2 promotes proliferation of glioma cells by forming a GPC1/c-Myc positive feedback loop, and ANXA2 together with its downstream target GPC1 could be a potential "combination biomarker" for predicting prognosis of glioma patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-021-03547-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954792PMC
March 2021

Synergistically enhance confined diffusion by continuum intersecting channels in zeolites.

Sci Adv 2021 Mar 12;7(11). Epub 2021 Mar 12.

State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, and Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, P. R. China.

In separation and catalysis applications, adsorption and diffusion are normally considered mutually exclusive. That is, rapid diffusion is generally accompanied by weak adsorption and vice versa. In this work, we analyze the anomalous loading-dependent mechanism of -xylene diffusion in a newly developed zeolite called SCM-15. The obtained results demonstrate that the unique system of "continuum intersecting channels" (i.e., channels made of fused cavities) plays a key role in the diffusion process for the molecule-selective pathways. At low pressure, the presence of strong adsorption sites and intersections that provide space for molecule rotation facilitates the diffusion of -xylene along the direction. Upon increasing the molecular uptake, the adsorbates move faster along the direction because of the effect of continuum intersections in reducing the diffusion barriers and thus maintaining the large diffusion coefficient of the diffusing compound. This mechanism synergistically improves the diffusion in zeolites with continuum intersecting channels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/sciadv.abf0775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954456PMC
March 2021

MgCoO@NiMn layered double hydroxide core-shell nanocomposites on nickel foam as superior electrode for all-solid-state asymmetric supercapacitors.

J Colloid Interface Sci 2021 Jun 7;592:455-467. Epub 2021 Feb 7.

College of Materials Science and Engineering, Institute for Graphene Applied Technology Innovation, Qingdao University, Qingdao 266071, China; College of Materials Science and Engineering, Linyi University, Linyi, 276000 Shandong, China. Electronic address:

In this work, MgCoO@NiMn layered double hydroxide (LDH) core-shell structured nanocomposites on Ni foam (NF) are synthesized by facile hydrothermal and calcination methods. MgCoO/NF is synthesized first via a hydrothermal reaction and annealing treatment, and then utilized to prepare MgCoO@NiMn-LDH/NF core-shell structured nanocomposites via the second hydrothermal process. It is found that the MgCoO@NiMn-LDH/NF nanocomposite prepared from 6 h hydrothermal reaction (MC@NM-LDH-2) exhibits an excellent specific capacitance of 3757.2 F g (at 1 A g). Moreover, a high capacitance retention (86.9% after 6000 cycles) and a low internal resistance (Rs) (0.565 Ω) can be achieved. Furthermore, an all-solid-state asymmetric supercapacitor (ASC) is assembled using MgCoO@NiMn-LDH/NF-2 as positive electrode and activated carbon (AC) as negative electrode. The as-fabricated MgCoO@NiMn-LDH/NF-2//AC ASC shows a high energy density of 62.33 Wh kg at 750 W kg. Meanwhile, the MgCoO@NiMn-LDH/NF-2//AC ASC device possesses an outstanding cycling stability of 93.7% retention of the initial capacitance after 6000 cycles and three ASC devices connected in series can light up a LED bulb for 15 min. Our results manifest that these core-shell structure MgCoO@NiMn-LDH nanocomposites could envision huge potential application in energy storage devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.02.011DOI Listing
June 2021

Identification and characterization of invertase family genes reveal their roles in vacuolar sucrose metabolism during Pyrus bretschneideri Rehd. fruit development.

Genomics 2021 Mar 8;113(3):1087-1097. Epub 2021 Mar 8.

Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address:

23 invertase (PbrInvs) genes, including eight vacuolar invertases (PbrvacInvs), five cell wall invertases (PbrcwInvs) and 10 alkaline/neutral invertases (PbrA/N-Invs), were identified from P. bretschneideri Rehd. genome, with diverse chromosome locations, cis-acting elements, gene structures and motifs. Their expression profiles were tissue-specific, and postharvest light or temperature treatment would alter their expression profiles. During 'Dangshansuli' pear development, in association with visual/inner quality change was the alternations of invertase activity and the expression profiles of PbrInvs. In combination with results of subcellular sugar distribution as well as correlation analysis among sugar content, invertase activity and PbrInv mRNA abundance, PbrvacInv1 might be involved in sucrose decomposition during pear development. PbrvacInv1-GFP fusion protein mainly accumulated on the tonoplast (vacuolar membrane); meanwhile, transient overexpression of PbrvacInv1 in pear fruit would upregulate vacInv activity, causing higher fructose and lower sucrose when compared with that of the control. Furthermore, invertase inhibitor 5 (PbrInvInh5) could interact with PbrvacInv1.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ygeno.2021.01.028DOI Listing
March 2021

Genetic Diversity of Hard Ticks (Acari: Ixodidae) in the South and East Regions of Kazakhstan and Northwestern China.

Korean J Parasitol 2021 Feb 19;59(1):103-108. Epub 2021 Feb 19.

Department of Basic Medicine, School of Medicine, Shihezi University, Shihezi City, Xinjiang Uygur Autonomous Region 832002, People's Republic of China.

To date, there is no report on the genetic diversity of ticks in these regions. A total of 370 representative ticks from the south and east regions of Kazakhstan (SERK) and Xinjiang Uygur Autonomous Region (XUAR) were selected for molecular comparison. A fragment of the mitochondrial cytochrome c oxidase subunit I (cox1) gene, ranging from 631 bp to 889 bp, was used to analyze genetic diversity among these ticks. Phylogenetic analyses indicated 7 tick species including Hyalomma asiaticum, Hyalomma detritum, Hyalomma anatolicum, Dermacentor marginatus, Rhipicephalus sanguineus, Rhipicephalus turanicus and Haemaphysalis erinacei from the SERK clustered together with conspecific ticks from the XUAR. The network diagram of haplotypes showed that i) Hy. asiaticum from Almaty and Kyzylorda Oblasts together with that from Yuli County of XUAR constituted haplogroup H-2, and the lineage from Chimkent City of South Kazakhstan was newly evolved; and ii) the R. turanicus ticks sampled in Israel, Almaty, South Kazakhstan, Usu City, Ulugqat and Baicheng Counties of XUAR were derivated from an old lineage in Alataw City of XUAR. These findings indicate that: i) Hy. asiaticum, R. turanicus and Ha. erinacei shared genetic similarities between the SERK and XUAR; and ii) Hy. marginatum and D. reticulatus show differences in their evolution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3347/kjp.2021.59.1.103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939963PMC
February 2021

Prevalence of Sleep Disturbances and Sleep Quality in Chinese Healthcare Workers During the COVID-19 Pandemic: A Systematic Review and Meta-Analysis.

Front Psychiatry 2021 18;12:646342. Epub 2021 Feb 18.

Department of Psychiatry, Chaohu Hospital of Anhui Medical University, Hefei, China.

Healthcare workers (HWs) experienced high levels of work stress during the COVID-19 pandemic, leading to a high risk of sleep disturbances. This meta-analysis aimed to explore the prevalence of sleep disturbances and sleep quality in Chinese HWs during the COVID-19 pandemic. English (PubMed, EMBASE, PsycINFO, Web of Science, and the Cochrane Library) and Chinese databases (WanFang, Chinese National Knowledge Infrastructure, and SinoMed) were systematically and independently searched for relevant studies published from December 1, 2019, to May 20, 2020. The pooled prevalence of sleep disturbances and sleep quality were calculated using a random-effects model. A total of 17 studies involving 12,682 Chinese HWs were included in the meta-analysis. The pooled prevalence of sleep disturbances in Chinese HWs was 45.1% (95% CI: 37.2-53.1%). We found that the prevalence of sleep disturbances varied among frontline, infected, and non-frontline HWs ( = 96.96, < 0.001); females and males ( = 9.10, = 0.003); studies using different assessment instruments ( = 96.05, < 0.001); and studies with different sample sizes ( = 5.77, = 0.016) and cut-off values ( = 62.28, < 0.001). The pooled mean total score of the Pittsburgh Sleep Quality Index (PSQI) was 9.83 (95% CI: 8.61-11.04). HWs in Wuhan had a higher total PSQI score than those in other regions ( = 9.21, = 0.002). Sleep disturbances were common in Chinese HWs during the COVID-19 pandemic, particularly in frontline and infected HWs. Our results indicate the heavy mental health burden on HWs during the COVID-19 pandemic in China and can provide other countries with valuable information to assist HWs during the crisis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyt.2021.646342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930820PMC
February 2021

Lhermitte-Duclos disease: A case report and literature review.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Feb;46(2):195-199

Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008.

Lhermitte-Duclos disease (LDD) is a type of rare brain tumor located in posterior fossa. A patient with LDD located in the left cerebellum and vermis was admitted by the Department of Neurosurgery, Xiangya Hospital, Central South University. MRI scan showed slightly heterogeneous enhancement at the region close to vermis. The patient underwent partial resection on August 11, 2016 without postoperative chemoradiotherapy. The progress free survival was 11 months and the overall survival was 17 months. What the case reveals is that the partial resection is not beneficial to these patients with LDD as the residual lesion probably recurs in a short term after operation. The pathogenesis, diagnosis and treatment of LDD are explored and summarized in combination with relevant literature.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.11817/j.issn.1672-7347.2021.190574DOI Listing
February 2021