Publications by authors named "Zhiqiang Jin"

52 Publications

Overexpression of a Banana Aquaporin Gene Enhances Tolerance to Multiple Abiotic Stresses in Transgenic Banana and Analysis of Its Interacting Transcription Factors.

Front Plant Sci 2021 25;12:699230. Epub 2021 Aug 25.

Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.

Aquaporins can improve the ability of plants to resist abiotic stresses, but the mechanism is still not completely clear. In this research, overexpression of in banana improved tolerance to multiple stresses. The transgenic plants resulted in lower ion leakage and malondialdehyde content, while the proline, chlorophyll, soluble sugar, and abscisic acid (ABA) contents were higher. In addition, under high salt and recovery conditions, the content of Na and K is higher, also under recovery conditions, the ratio of K/Na is higher. Finally, under stress conditions, the expression levels of ABA biosynthesis and response genes in the transgenic lines are higher than those of the wild type. In previous studies, we proved that the MaMADS3 could bind to the promoter region of , thereby regulating the expression of and affecting the drought tolerance of banana plants. However, the mechanism of gene response to stress under different adversity conditions might be regulated differently. In this study, we proved that some transcription factor genes, including MaERF14, MaDREB1G, MaMYB1R1, MaERF1/39, MabZIP53, and MaMYB22, showed similar expression patterns with under salt or cold stresses, and their encoded proteins could bind to the promoter region of . Here we proposed a novel -mediated mechanism that enhanced salt and cold tolerance in bananas. The results of this study have enriched the stress-resistant regulatory network of aquaporins genes and are of great significance for the development of molecular breeding strategies for stress-resistant fruit crops.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.699230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424054PMC
August 2021

MaMADS36 plays a central role in regulating banana fruit ripening.

J Exp Bot 2021 Jul 19. Epub 2021 Jul 19.

Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, 4 Xueyuan Road, 571101 Haikou, China.

Bananas are model fruits for studying starch conversion and climactericity. Starch degradation and ripening are two important biological processes that occur concomitantly in banana fruit. Ethylene biosynthesis, and postharvest fruit ripening processes, i.e., starch degradation, fruit softening, and sugar accumulation are highly correlated and thus could be controlled by a common regulatory switch. However, this switch has not been identified. In this study, we transformed red banana with sense and anti-sense constructs of the MaMADS36 transcription factor (also MuMADS1, Ma05_g18560.1). Analysis of these lines showed that MaMADS36 interacts with 74 other proteins to form a co-expression network and could act as an important switch to regulate ethylene biosynthesis, starch degradation, softening, and sugar accumulation. Among these target genes, musa acuminata beta-amylase 9b (MaBAM9b, Ma05_t07800.1) that encodes a starch degradation enzyme, was selected to further investigate the regulatory mechanism of MaMADS36. Our findings indicated that MaMADS36 directly binds the CA/T(r)G box of the MaBAM9b promoter to increase MaBAM9b transcription and in turn, enzyme activity, and starch degradation during ripening. These results will help to further our understanding of the fine regulatory mechanisms of MADS-box transcription factors in regulating fruit ripening, which can be applied to breeding programs to improve fruit shelf-life.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jxb/erab341DOI Listing
July 2021

Molecular identification of the key starch branching enzyme-encoding gene SBE2.3 and its interacting transcription factors in banana fruits.

Hortic Res 2020 Jul 1;7(1):101. Epub 2020 Jul 1.

Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, 571101, Haikou, People's Republic of China.

Starch branching enzyme (SBE) has rarely been studied in common starchy banana fruits. For the first time, we report here the molecular characterization of seven SBE (MaSBE) and six SBE (MbSBE) genes in the banana A- and B-genomes, respectively, which could be classified into three distinct subfamilies according to genome-wide identification. Systematic transcriptomic analysis revealed that six MaSBEs and six MbSBEs were expressed in the developing banana fruits of two different genotypes, BaXi Jiao (BX, AAA) and Fen Jiao (FJ, AAB), among which MaSBE2.3 and MbSBE2.3 were highly expressed. Transient silencing of MaSBE2.3 expression in banana fruit discs led to a significant decrease in its transcription, which coincides with significant reductions in total starch and amylopectin contents compared to those of empty vector controls. The suggested functional role of MaSBE2.3 in banana fruit development was corroborated by its transient overexpression in banana fruit discs, which led to significant enhancements in total starch and amylopectin contents. A number of transcription factors, including three auxin response factors (ARF2/12/24) and two MYBs (MYB3/308), that interact with the MaSBE2.3 promoter were identified by yeast one-hybrid library assays. Among these ARFs and MYBs, MaARF2/MaMYB308 and MaARF12/MaARF24/MaMYB3 were demonstrated via a luciferase reporter system to upregulate and downregulate the expression of MaSBE2.3, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41438-020-0325-1DOI Listing
July 2020

Genome-Wide Identification and Transcript Analysis of TCP Gene Family in Banana (Musa acuminata L.).

Biochem Genet 2021 Jun 22. Epub 2021 Jun 22.

Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, 4 Xueyuan Road, Haikou, 571101, China.

Plant-specific TEOSINTE-BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR1 (TCP) gene family has versatile functions in diverse aspects of plants. However, less research on banana TCPs was done comprehensively. Accordingly, 48 banana TCP genes were characterized on aspects of gene structure, conserved motifs, phylogenetic relationship, and expression patterns. Members of the MaTCP gene family were unevenly distributed among 11 chromosomes and purification selection was the driving force of the MaTCP gene family. Gene duplication analysis indicated that segmental duplication is the major contributor to family expansion. Promoter analysis showed that MaTCPs might be involved in banana growth, development, and abiotic stress responses. Further, the expression of 12 MaTCPs was analyzed by real-time quantitative RT-PCR, and the protein interaction analysis showed that MaPCF10 and MaPCF13 may have an important function in banana fruit development and ripening. These results lay the foundation for further study of the functions of TCP genes in banana.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10528-021-10100-8DOI Listing
June 2021

Hierarchical CoFe LDH/MOF nanorods array with strong coupling effect grown on carbon cloth enables efficient oxidation of water and urea.

Nanotechnology 2021 Jul 2;32(38). Epub 2021 Jul 2.

School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, People's Republic of China.

Oxygen evolution reaction (OER) and urea oxidation reaction (UOR) play important roles in the fields of hydrogen energy production and pollution treatment. Herein, a facile one-step chemical etching strategy is provided for fabricating one-dimensional hierarchical nanorods array composed of CoFe layered double hydroxide (LDH)/metal-organic frameworks (MOFs) supported on carbon cloth as efficient and stable OER and UOR catalysts. By precisely controlling the etching rate, the ligands from Co-MOFs are partially removed, the corresponding metal centers then coordinate with hydroxyl ions to generate ultrathin amorphous CoFe LDH nanosheets. The resultant CoFe LDH/MOFs catalyst possesses large active surface area, enhanced conductivity and extended electron/mass transfer channels, which are beneficial for catalytic reactions. Additionally, the intimate contact between CoFe LDH and MOFs modulates the local electronic structure of the catalytic active site, leading to enhanced adsorption of oxygen-containing intermediates to facilitate fast electrocatalytic reaction. As a result, the optimized CoFe LDH/MOF-0.06 exhibits superior OER activity with a low overpotential of 276 at a current density of 10 mA cmwith long-term durability. Additionally, it merely requires a voltage of 1.45 V to obtain 10 mA cmin 1 M KOH solution with 0.33 urea and is 56 mV lower than the one in pure KOH. The work presented here may hew out a brand-new route to construct multi-functional electrocatalysts for water splitting, COreduction, nitrogen reduction reactions and so on.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ac0b65DOI Listing
July 2021

Antibiotic Zwitterionic Nanogel Membrane: from Molecular Dynamics Simulation to Structure Manipulation.

ACS Appl Mater Interfaces 2021 Apr 12;13(15):18237-18246. Epub 2021 Apr 12.

Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.

Membrane separation has been considered as one of the most revolutionary technologies for the removal of oils, dyes, or other pollutants from wastewater. However, most membranes still face great challenges in water permeability, antifouling property, and even antibiotic ability. Possessing a pathogen-repellent surface is of great significance as it can enable membranes to minimize the presence of active viral pathogens. Herein, we demonstrate a distinct design with a molecular dynamics simulation-guided experiment for the surface domination of antibiotic zwitterionic nanogel membranes. The zwitterionic nanoparticle gel (ZNG)/Cu/glutaraldehyde (GA) synergy system is first simulated by introducing a ZNG into a preset CuCl brine solution and into a GA ethanol solution, in which the nanogel is observed to initially swell and subsequently shrink with the increase of GA concentration, leading to the membrane surface structure transition. Then, the corresponding experiments are performed under strict conditions, and the results suggest the surface structure transition from nanoparticles to network nanoflowers, which are consistent with the simulated results. The obtained network structure membrane with superhydrophilic and underwater superoleophobic abilities can significantly enhance the water permeability as high as almost 40% with its original rejection rate in comparison with unoptimizable ZNG-PVDF (polyvinylidene difluoride) membranes. Moreover, the obtained membrane achieves additional excellent antibiofouling capacity with the antibiotic efficiency exceeding 99.3%, manifesting remarkable potential for disinfection applications. By comparison, the conventional antibiotic methods generally improve the membrane's antibiotic property solely but can hardly improve the other properties of the membrane. That is to say, our simulation combined with the experimental strategy significantly improved the zwitterionic membrane property in this work, which provides a new perspective on the design of high-performance functional materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c00378DOI Listing
April 2021

Identification and expression of the BAHD family during development, ripening, and stress response in banana.

Mol Biol Rep 2021 Feb 25;48(2):1127-1138. Epub 2021 Jan 25.

Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.

The BAHD family is involved in different biological roles in plants, including secondary metabolite synthesis, improving abiotic/biotic stress resistance, and influencing fruit quality. However, the knowledge about BAHD in banana, an important fruit crop, is limited. In this study, 46 banana BAHD genes (MaBAHDs) were identified and divided into four groups according to phylogenetic analysis. Most of the MaBAHD genes in the same group presented similar conserved motifs and genetic structures. MaBAHD genes have similar expression patterns in two banana varieties, and more genes showed high expressions in the roots. The comprehensive MaBAHD gene expression patterns obtained from two varieties of banana showed valuable information regarding their participation in fruit development, ripening, and response to abiotic/biotic stresses, suggesting that they play key roles in these processes. The systematic analysis of MaBAHD genes offered basic insight for further gene functional assays and potential applications in genetically improving banana cultivars.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11033-020-06132-9DOI Listing
February 2021

Characteristics of banana B genome MADS-box family demonstrate their roles in fruit development, ripening, and stress.

Sci Rep 2020 11 30;10(1):20840. Epub 2020 Nov 30.

Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, 4 Xueyuan Road, Haikou, 571101, China.

MADS-box genes are critical regulators of growth and development in flowering plants. Sequencing of the Musa balbisiana (B) genome has provided a platform for the systematic analysis of the MADS-box gene family in the important banana ancestor Musa balbisiana. Seventy-seven MADS-box genes, including 18 type I and 59 type II, were strictly identified from the banana (Pisang Klutuk Wulung, PKW, 2n = 2x = 22) B genome. These genes have been preferentially placed on the banana B genome. Evolutionary analysis suggested that M. balbisiana MCM1-AGAMOUS-DEFICIENS-SRF (MbMADS) might be organized into the MIKC, MIKC*, Mα, Mβ, and Mγ groups according to the phylogeny. MIKC was then further categorized into 10 subfamilies according to conserved motif and gene structure analyses. The well-defined MADS-box genes highlight gene birth and death in banana. MbMADSes originated from the same ancestor as MaMADSes. Transcriptome analysis in cultivated banana (ABB) revealed that MbMADSes were conserved and differentially expressed in several organs, in various fruit developing and ripening stages, and in stress treatments, indicating the participation of these genes in fruit development, ripening, and stress responses. Of note, SEP/AGL2 and AG, as well as other several type II MADS-box genes, including the STMADS11 and TM3/SOC1 subfamilies, indicated elevated expression throughout banana fruit development, ripening, and stress treatments, indicating their new parts in controlling fruit development and ripening. According to the co-expression network analysis, MbMADS75 interacted with bZIP and seven other transcription factors to perform its function. This systematic analysis reveals fruit development, ripening, and stress candidate MbMADSes genes for additional functional studies in plants, improving our understanding of the transcriptional regulation of MbMADSes genes and providing a base for genetic modification of MADS-mediated fruit development, ripening, and stress.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-77870-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705751PMC
November 2020

Comprehensive ESI-Q TRAP-MS/MS based characterization of metabolome of two mango (Mangifera indica L) cultivars from China.

Sci Rep 2020 11 18;10(1):20017. Epub 2020 Nov 18.

Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.

Polyphenols based bioactive compounds from vegetables and fruits are known for impressive antioxidant activity. Ingestion of these antioxidants may promote human health against cardiovascular diseases and cancer. Mango is a popular tropical fruit with special taste, high nutritional value and health-enhancing metabolites. The aim was to investigate the diversity of phytochemicals between two mango cultivars of china at three stages of fruit maturity. We used ESI-QTRAP-MS/MS approach to characterize comprehensively the metabolome of two mango cultivars named Hongguifei (HGF) and Tainong (TN). HPLC was used to quantify selected catechin based phenolic compounds. Moreover, real-time qPCR was used to study the expression profiles of two key genes (ANR and LAR) involved in proanthocyanidin biosynthesis from catechins and derivatives. A total of 651 metabolites were identified, which include at least 257 phenolic compounds. Higher number of metabolites were differentially modulated in peel as compared to pulp. Overall, the relative quantities of amino acids, carbohydrates, organic acids, and other metabolites were increased in the pulp of TN cultivar. While the contents of phenolic compounds were relatively higher in HGF cultivar. Moreover, HPLC based quantification of catechin and derivatives exhibited cultivar specific variations. The ANR and LAR genes exhibited an opposite expression profile in both cultivars. Current study is the first report of numerous metabolites including catechin-based derivatives in mango fruit. These findings open novel possibilities for the use of mango as a source of bioactive compounds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-75636-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676270PMC
November 2020

Molecular identification of the key starch branching enzyme-encoding gene and its interacting transcription factors in banana fruits.

Hortic Res 2020 1;7:101. Epub 2020 Jul 1.

Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, 571101 Haikou, People's Republic of China.

Starch branching enzyme (SBE) has rarely been studied in common starchy banana fruits. For the first time, we report here the molecular characterization of seven () and six () genes in the banana A- and B-genomes, respectively, which could be classified into three distinct subfamilies according to genome-wide identification. Systematic transcriptomic analysis revealed that six s and six s were expressed in the developing banana fruits of two different genotypes, BaXi Jiao (BX, AAA) and Fen Jiao (FJ, AAB), among which and were highly expressed. Transient silencing of expression in banana fruit discs led to a significant decrease in its transcription, which coincides with significant reductions in total starch and amylopectin contents compared to those of empty vector controls. The suggested functional role of in banana fruit development was corroborated by its transient overexpression in banana fruit discs, which led to significant enhancements in total starch and amylopectin contents. A number of transcription factors, including three auxin response factors (ARF2/12/24) and two MYBs (MYB3/308), that interact with the promoter were identified by yeast one-hybrid library assays. Among these ARFs and MYBs, MaARF2/MaMYB308 and MaARF12/MaARF24/MaMYB3 were demonstrated via a luciferase reporter system to upregulate and downregulate the expression of , respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41438-020-0325-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326998PMC
July 2020

Identification of transcription factors interacting with a 1274 bp promoter of MaPIP1;1 which confers high-level gene expression and drought stress Inducibility in transgenic Arabidopsis thaliana.

BMC Plant Biol 2020 Jun 16;20(1):278. Epub 2020 Jun 16.

Key Laboratory of Genetic Improvement of Bananas, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.

Background: Drought stress can severely affect plant growth and crop yield. The cloning and identification of drought-inducible promoters would be of value for genetically-based strategies to improve resistance of crops to drought.

Results: Previous studies showed that the MaPIP1;1 gene encoding an aquaporin is involved in the plant drought stress response. In this study, the promoter pMaPIP1;1, which lies 1362 bp upstream of the MaPIP1;1 transcriptional initiation site, was isolated from the banana genome..And the transcription start site(A) is 47 bp before the ATG. To functionally validate the promoter, various lengths of pMaPIP1;1 were deleted and fused to GUS to generate pMaPIP1;1::GUS fusion constructs that were then transformed into Arabidopsis to generate four transformants termed M-P1, M-P2, M-P3 and M-P4.Mannitol treatment was used to simulate drought conditions. All four transformants reacted well to mannitol treatment. M-P2 (- 1274 bp to - 1) showed the highest transcriptional activity among all transgenic Arabidopsis tissues, indicating that M-P2 was the core region of pMaPIP1;1. This region of the promoter also confers high levels of gene expression in response to mannitol treatment. Using M-P2 as a yeast one-hybrid bait, 23 different transcription factors or genes that interacted with MaPIP1;1 were screened. In an dual luciferase assay for complementarity verification, the transcription factor MADS3 positively regulated MaPIP1;1 transcription when combined with the banana promoter. qRT-PCR showed that MADS3 expression was similar in banana leaves and roots under drought stress. In banana plants grown in 45% soil moisture to mimic drought stress, MaPIP1;1 expression was maximized, which further demonstrated that the MADS3 transcription factor can synergize with MaPIP1;1.

Conclusions: Together our results revealed that MaPIP1;1 mediates molecular mechanisms associated with drought responses in banana, and will expand our understanding of how AQP gene expression is regulated. The findings lay a foundation for genetic improvement of banana drought resistance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12870-020-02472-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7298759PMC
June 2020

Genome-Wide Analysis of Basic Helix-Loop-Helix Transcription Factors to Elucidate Candidate Genes Related to Fruit Ripening and Stress in Banana ( L. AAA Group, cv. Cavendish).

Front Plant Sci 2020 27;11:650. Epub 2020 May 27.

Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Hainan, China.

The basic helix-loop-helix (bHLH) proteins are a superfamily of transcription factors (TFs) that can bind to specific DNA target sites, playing a central role in a wide range of metabolic, physiological, and developmental processes in higher organisms. However, no systemic analysis of bHLH TFs has been reported in banana, a typical climacteric fruit in tropical and subtropical regions. In our study, 259 MabHLH TF genes were identified in the genome of (A genome), and phylogenetic analysis indicated that these MabHLHs could be classified into 23 subfamilies with the bHLHs from rice and . The amino acid sequences of the bHLH domain in all MabHLH protein sequences were quite conserved, especially Arg-12, Arg-13, Leu-23, and Leu-79. Distribution mapping results showed that 258 MabHLHs were localized on the 11 chromosomes in the . genome. The results indicated that 40.7% of gene duplication events were located in collinear fragments, and segmental duplications might have played a key role in the expansion of . Moreover, the expression profiles of in different fruit development and ripening stages and under various abiotic and biotic stresses were investigated using available RNA-sequencing data to obtain fruit development, ripening-specific, and stress-responsive candidate genes. Finally, a co-expression network of was constructed by weighted gene co-expression network analysis to elucidate the that might participate in important metabolic biosynthesis pathways in banana during development and the response to stress. A total of 259 were identified, and their sequence features, conserved domains, phylogenetic relationships, chromosomal distributions, gene duplications, expression profiles, and co-expression networks were investigated. This study systematically identified the in the . genome at the genome-wide level, providing important candidate genes for further functional analysis. These findings improve our understanding of the molecular basis of developmental and stress tolerance in an important banana cultivar.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2020.00650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7267074PMC
May 2020

Effect of cultivating Pleurotus ostreatus on substrates supplemented with herb residues on yield characteristics, substrates degradation, and fruiting bodies' properties.

J Sci Food Agric 2020 Oct 23;100(13):4901-4910. Epub 2020 Jun 23.

Department of Biological Science and Technology, Changzhi University, Changzhi, Shanxi, 046011, China.

Background: Inappropriate disposal of herb residues in China has caused major problems for the immediate environment and to human safety. Here, three herb residues, compound Kushen injection (CKI), Qizhi Tongluo capsule (QTC), and Shenbai Shuxin capsule (SSC), were applied as substrates to corncob at various ratios (30:60, 45:45, and 60:30) for the propagation of the mushroom Pleurotus ostreatus. The effects of supplementation using herb residues on yield, biodegradation ability, bioactive compounds, antioxidant properties, and safety of P. ostreatus were assessed.

Results: Different spawn running times were observed using growth medium, whereas 45CKI, 60QTC, and 30SSC media were determined as optimal-performing substrate combinations, resulting in yields of 843 g kg , 828 g kg , and 715 g kg respectively. Biodegradation analysis of consumed substrates revealed a significant decrease in cellulose and hemicellulose levels compared with lignin. Furthermore, chemical analysis of fruiting bodies revealed that the 45CKI and 60QTC substrates resulted in higher total phenol, flavonoid, terpenoid, and vitamin C levels, but significantly reduced water-soluble polysaccharides compared with the corncob medium. The methanol extract of fruiting bodies grown on substrates containing herb residues exhibited higher antioxidant properties than the control, as it was more effective in scavenging 2,2-diphenyl-1-picrylhydrazyl radicals, had greater reducing power, and more strongly inhibiting lipid peroxidation. Furthermore, high-performance liquid chromatography studies indicated that fruiting bodies did not generate matrine (a specific toxin produced in Kushen) when cultivated using the CKI substrate.

Conclusions: P. ostreatus cultivation on substrates mixed with herb residues facilitates herb residue management as well as bioactivity-rich and non-toxic fruit body formation. © 2020 Society of Chemical Industry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jsfa.10551DOI Listing
October 2020

Robust Silica-Cellulose Composite Aerogels with a Nanoscale Interpenetrating Network Structure Prepared Using a Streamlined Process.

Polymers (Basel) 2020 Apr 3;12(4). Epub 2020 Apr 3.

School of Chemistry and Chemical Engineering, Inner Mongolia University of Science & Technology, Baotou 014010, China.

Silica aerogels can be strengthened by forming a nanoscale interpenetrating network (IPN) comprising a silica gel skeleton and a cellulose nanofiber network. Previous studies have demonstrated the effectiveness of this method for improving the mechanical properties and drying of aerogels. However, the preparation process is generally tedious and time-consuming. This study aims to streamline the preparation process of these composite aerogels. Silica alcosols were directly diffused into cellulose wet gels with loose, web-like microstructures, and an IPN structure was gradually formed by regulating the gelation rate. Supercritical CO drying followed to obtain composite aerogels. The mechanical properties were further enhanced by a simple secondary regulation process that increased the quantity of bacterial cellulose (BC) nanofibers per unit volume of the matrix. This led to the production of aerogels with excellent bendability and a high tensile strength. A maximum breaking stress and tensile modulus of 3.06 MPa and 46.07 MPa, respectively, were achieved. This method can be implemented to produce robust and bendable silica-based composite aerogels (CAs).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym12040807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240684PMC
April 2020

Genomic and Transcriptional Analysis of Banana Ovate Family Proteins Reveals Their Relationship with Fruit Development and Ripening.

Biochem Genet 2020 Jun 6;58(3):412-429. Epub 2020 Mar 6.

Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, 4 Xueyuan Road, Haikou, 571101, China.

Ovate Family Proteins (OFPs) belong to a plant-specific transcription factor family. They have been found to have significant roles in growth and development in Arabidopsis and tomato; however, little is known regarding their role in banana. Thus, a genome-wide study of OFP genes in banana was conducted for the first time in the present study. The results demonstrated that 49 OFP family members are unequally distributed across 11 chromosomes. Phylogenetic analysis grouped these genes into two subfamilies and eight subgroups, which was confirmed by the conserved motif and gene structure analysis. Furthermore, MaOFPs genes duplicates were found to have originated from whole-genome duplication (WGD). The expression patterns of the genes in the various tissues and at different fruit development and ripening stages in the BaXi Jiao (BX) and Feng Jiao (FJ), banana cultivars were elucidated using transcriptome analysis. Using co-expression network analysis, MaOFP1 was found to interact not only with MaMADS36 but also with hormone response proteins. These findings improve our understanding of the functions of MaOFPs genes in the control of plant hormone signal transduction pathways during banana growth and ripening, which should inform the genetic improvement of important agricultural characters.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10528-020-09951-4DOI Listing
June 2020

An aquaporin gene MaPIP2-7 is involved in tolerance to drought, cold and salt stresses in transgenic banana (Musa acuminata L.).

Plant Physiol Biochem 2020 Feb 12;147:66-76. Epub 2019 Dec 12.

Key Laboratory of Genetic Improvement of Bananas, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, China; Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, China; Hainan Academy of Tropical Agricultural Resource, Haikou, Hainan, 571101, China. Electronic address:

Aquaporins (AQPs) transport water and other small molecules; however, their precise role in abiotic stress responses is not fully understood. In this study, we cloned and characterized the PIP2 group AQP gene, MaPIP2-7, in banana. MaPIP2-7 expression was upregulated after osmotic (mannitol), cold, and salt treatments. Overexpression of MaPIP2-7 in banana improved tolerance to multiple stresses such as drought, cold, and salt. MaPIP2-7 transgenic plants showed lower levels of malondialdehyde (MDA) and ion leakage (IL), but higher contents of chlorophyll, proline, soluble sugar, and abscisic acid (ABA) compared with wild type (WT) plants under stress and recovery conditions. Additionally, MaPIP2-7 overexpression decreased cellular contents of Na and K under salt and recovery conditions, and produced an elevated K/Na ratio under recovery conditions. Finally, ABA biosynthetic and responsive genes exhibited higher expression levels in transgenic lines relative to WT under stress conditions. Taken together, our results demonstrate that MaPIP2-7 confers tolerance to drought, cold, and salt stresses by maintaining osmotic balance, reducing membrane injury, and improving ABA levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plaphy.2019.12.011DOI Listing
February 2020

Musa balbisiana genome reveals subgenome evolution and functional divergence.

Nat Plants 2019 08 15;5(8):810-821. Epub 2019 Jul 15.

CIRAD, UMR BGPI, Montpellier, France.

Banana cultivars (Musa ssp.) are diploid, triploid and tetraploid hybrids derived from Musa acuminata and Musa balbisiana. We presented a high-quality draft genome assembly of M. balbisiana with 430 Mb (87%) assembled into 11 chromosomes. We identified that the recent divergence of M. acuminata (A-genome) and M. balbisiana (B-genome) occurred after lineage-specific whole-genome duplication, and that the B-genome may be more sensitive to the fractionation process compared to the A-genome. Homoeologous exchanges occurred frequently between A- and B-subgenomes in allopolyploids. Genomic variation within progenitors resulted in functional divergence of subgenomes. Global homoeologue expression dominance occurred between subgenomes of the allotriploid. Gene families related to ethylene biosynthesis and starch metabolism exhibited significant expansion at the pathway level and wide homoeologue expression dominance in the B-subgenome of the allotriploid. The independent origin of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) homoeologue gene pairs and tandem duplication-driven expansion of ACO genes in the B-subgenome contributed to rapid and major ethylene production post-harvest in allotriploid banana fruits. The findings of this study provide greater context for understanding fruit biology, and aid the development of tools for breeding optimal banana cultivars.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41477-019-0452-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784884PMC
August 2019

Identification, Expression, and Interaction Network Analyses of the CDPK Gene Family Reveal Their Involvement in the Development, Ripening, and Abiotic Stress Response in Banana.

Biochem Genet 2020 Feb 29;58(1):40-62. Epub 2019 May 29.

Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China.

Calcium-dependent protein kinases (CDPKs) play vital roles in the regulation of plant growth, development, and tolerance to various abiotic stresses. However, little information is available for this gene family in banana. In this study, 44 CDPKs were identified in banana and were classified into four groups based on phylogenetic, gene structure, and conserved motif analyses. The majority of MaCDPKs generally exhibited similar expression patterns in the different tissues. Transcriptome analyses revealed that many CDPKs showed strong transcript accumulation at the early stages of fruit development and postharvest ripening in both varieties. Interaction network and co-expression analysis further identified some CDPKs-mediated network that was potentially active at the early stages of fruit development. Comparative expression analysis suggested that the high levels of CDPK expression in FJ might be related to its fast ripening characteristic. CDPK expression following the abiotic stress treatments indicated a significant transcriptional response to osmotic, cold, and salt treatment, as well as differential expression profiles, between BX and FJ. The findings of this study elucidate the transcriptional control of CDPKs in development, ripening, and the abiotic stress response in banana. Some tissue-specific, development/ripening-dependent, and abiotic stress-responsive candidate MaCDPK genes were identified for further genetic improvement of banana.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10528-019-09916-2DOI Listing
February 2020

Volumetric enhancement of Raman scattering for fast detection based on a silver-lined hollow-core fiber.

Opt Express 2019 Apr;27(7):10370-10382

Fast detection and identification of chemicals are of utmost importance for field testing and real-time monitoring in many fields. Raman spectroscopy is the predominant technique in principle, but its wide application is limited on account of weak scattering efficiency. Surface Enhanced Raman Spectroscopy (SERS) technique provides a solution for signal enhancement, but may not good at fast detection due to cross contamination and bulky instruments. Hollow-core fiber-based Raman cell with long interaction length can achieve high detection sensitivity, but it also suffers from low flow rate, bulky high-pressure equipment and light coupling structure, which also restricts its application for fast detection. In order to solve those problems, we proposed a portable Raman cell, by using metal-lined hollow-core fibers (MLHCF) with large bandwidth, good field confinement, extremely large numerical aperture and arbitrary length. With our proposed fiber inserted light coupling and light reflecting method, a Raman cell of 3.1 cm in length provides nearly 50 times of signal enhancement compared with direct detection using bare fiber tip. Furthermore, the sample exchange rate could be as fast as 1 second even under normal pressure without any cross contamination. At last, we also demonstrated the underlying general mechanism of signal enhancement and summarized it as volumetric enhancement of Raman scattering (VERS). Both the experiment results and the theoretical analysis demonstrated that our device has the potential for fast online Raman detection, which also possesses high-sensitivity and high-accuracy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.27.010370DOI Listing
April 2019

Understanding the Antifouling Mechanism of Zwitterionic Monomer-Grafted Polyvinylidene Difluoride Membranes: A Comparative Experimental and Molecular Dynamics Simulation Study.

ACS Appl Mater Interfaces 2019 Apr 2;11(15):14408-14417. Epub 2019 Apr 2.

Beijing Key Laboratory of Ionic Liquids Clean Process, CAS Key Laboratory of Green Process and Engineering, State Key Laboratory of Multiphase Complex Systems , Institute of Process Engineering , Beijing 100190 , People's Republic of China.

The antifouling process of the membrane is very vital for the highly efficient treatment of industrial wastewater, especially high salinity wastewater containing oil and other pollutants. In the present work, the dynamical antifouling mechanism is explored via molecular dynamics simulations, while the corresponding experiments about surface properties of the zwitterionic monomer-grafted polyvinylidene difluoride membrane are designed to verify the simulated mechanism. Water can form a stable hydration layer at the grafted membrane surface, where all the simulated radial distribution function of water/membrane, hydrogen bond number, water diffusivity, and experimental oil contact angles are stable. However, the water flux across the membrane will increase first and then decrease as the grafting ratio increases, which not only depends on the reduced pore size of the zwitterionic monomer-grafted membrane but also results from water diffusion. Furthermore, the dynamical fouling processes of pollutants (taking sodium alginate as an example) on the grafted membrane in water and brine solution are investigated, where both the high grafting ratio and electrolyte CaCl can enhance the fouling energy barrier of the pollutant. The results show that both the enhanced hydrophilic property and the electrostatic repulsion can affect the antifouling capability of the grafted membrane. Finally, the ternary synergistic antifouling mechanisms among the zwitterionic membrane, electrolyte, and pollutant sodium alginates are discussed, which could be helpful for the rational design and preparation of new and highly efficient zwitterionic antifouling membranes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.8b22059DOI Listing
April 2019

Identification and characterization of miRNA169 family members in banana ( L.) that respond to sp. infection in banana cultivars.

PeerJ 2018 21;6:e6209. Epub 2018 Dec 21.

Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.

MicroRNAs (miRNAs) play an important role in plant resistance to pathogen infections. However, little is known about the role of miRNAs in banana Fusarium wilt, which is the most economically devastating disease in banana production. In the present study, we identified and characterized a total of 18 miR169 family members in banana ( L.) based on small RNA sequencing. The banana miR169 family clustered into two groups based on miRNA evolutionary analysis. Multiple sequence alignment indicated a high degree of sequence conservation in miRNA169 family members across 28 plant species. Computational target prediction algorithms were used to identify 25 targets of miR169 family members in banana. These targets were enriched in various metabolic pathways that include the following molecules: glycine, serine, threonine, pentose, glycerolipids, nucleotide sugars, starch, and sucrose. Through miRNA transcriptomic analysis, we found that ma-miR169a and ma-miR169b displayed high expression levels, whereas the other 16 ma-miR169 members exhibited low expression in the HG and Baxi banana cultivars. Further experiments indicate that there were negative relationships between ma-miR169a, ma-miR169b and their targets basing on their expression levels to Foc4 ( sp. tropical race 4) infection in resistant cultivars. But they were low expressed in susceptive cultivars. These results suggested that the expression levels of ma-miR169a and ma-miR169b were consistent with the resistance degree of the banana cultivars to Foc4. The analysis presented here constitutes a starting point to understand ma-miR169-mediated Fusarium wilt resistance at the transcriptional level in banana and predicts possible candidate targets for the genetic improvement of banana resistance to Foc4.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.6209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305118PMC
December 2018

WNT6 is an effective marker for osteosarcoma diagnosis and prognosis.

Medicine (Baltimore) 2018 Nov;97(46):e13011

Department of Orthopaedics, Pangang Group General Hospital, Panzhihua, Sichuan, China.

Wingless-Type MMTV Integration Site Family, Member 6 (WNT6) is a member of the Wnt family and its expression is abnormal in different human cancer cell lines. The purpose of this study was to investigate the clinical significance of WNT6 in osteosarcoma.The levels of WNT6 mRNA and protein in tissue and serum were detected through quantitative real-time polymorperase chain reaction (qRT-PCR) and Enzyme Lined Immunosorbent Assay (ELISA), respectively. Chi-square test was performed to estimate the association of WNT6 expression with clinical parameters among osteosarcoma patients. Receiver operation characteristic (ROC) curve was plotted to determine diagnostic performance of serum WNT6 in osteosarcoma. Survival analysis was performed using Kaplan-Meier method. Cox regression analysis was adopted to evaluate prognostic significance of WNT6 expression among osteosarcoma patients.Compared with the controls, WNT6 mRNA and protein levels were significantly elevated in patients with osteosarcoma (P > .05 for all). Furthermore, WNT6 upregulation showed positive correlation with patients' age (P < .001), tumor grade (P < .001) and distant metastasis (P = .001). WNT6 might be a diagnostic marker for osteosarcoma with an AUC of 0.854 combining a specificity of 88.4% and a sensitivity of 77.8%. Survival analysis result indicated that high WNT6 expression predicted poor survival (log rank test, P = .001). WNT6 might be a potential prognostic biomarker for osteosarcoma (HR = 2.227, 95%CI = 1.061-10.842, P = .027).WNT6 may be a diagnostic and prognostic marker in osteosarcoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000013011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257418PMC
November 2018

Overexpression of a Novel Gene from the Banana () Confers Increased Salt Stress Tolerance.

Int J Mol Sci 2018 Oct 11;19(10). Epub 2018 Oct 11.

Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Xueyuan Road 4, Haikou 571101, China.

Rho-like GTPases from plants (ROPs) are plant-specific molecular switches that are crucial for plant survival when subjected to abiotic stress. We identified and characterized 17 novel ROP proteins from (MaROPs) using genomic techniques. The identified MaROPs fell into three of the four previously described ROP groups (Groups II⁻IV), with MaROPs in each group having similar genetic structures and conserved motifs. Our transcriptomic analysis showed that the two banana genotypes tested, Fen Jiao and BaXi Jiao, had similar responses to abiotic stress: Six genes (, , , , , and ) were highly expressed in response to cold, salt, and drought stress conditions in both genotypes. Of these, was most highly expressed in response to salt stress. Co-localization experiments showed that the MaROP5g protein was localized at the plasma membrane. When subjected to salt stress, transgenic overexpressing had longer primary roots and increased survival rates compared to wild-type . The increased salt tolerance conferred by might be related to reduced membrane injury and the increased cytosolic K⁺/Na⁺ ratio and Ca concentration in the transgenic plants as compared to wild-type. The increased expression of salt overly sensitive (SOS)-pathway genes and calcium-signaling pathway genes in -overexpressing reflected the enhanced tolerance to salt stress by the transgenic lines in comparison to wild-type. Collectively, our results suggested that abiotic stress tolerance in banana plants might be regulated by multiple , and that might enhance salt tolerance by increasing root length, improving membrane injury and ion distribution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms19103108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213407PMC
October 2018

Yield, Nutritional Content, and Antioxidant Activity of on Corncobs Supplemented with Herb Residues.

Mycobiology 2018 29;46(1):24-32. Epub 2018 Mar 29.

Department of Pharmaceutical and Food Engineering, Shanxi University of Traditional Chinese Medicine, Yuci, Shanxi, China.

Improper disposal of herb residues in China has caused severe problems to the surrounding environment and human safety. Three herb residues, i.e., compound Kushen injection residues (CKI) and part one and part two of Qizhitongluo Capsule residues (QC1 and QC2, respectively), were used for the cultivation of . The effect of the supplementation of corncobs (CC) with different herb residues on yield, nutritional composition, and antioxidant activity of was investigated. Compared to the control, the higher mycelial growth rate was observed on substrates CC +30% CKI and CC +30% QC1, while the higher yield was obtained from substrates CC +30% QC2 and CC +30% CKI. Moreover, chemical analysis of fruit bodies revealed that the addition of herb residues to CC significantly increased proteins, amino acids, ashes, minerals (Na and Ca), and total phenolic contents but significantly reduced carbohydrates and IC values of DPPH radicals. In addition, no heavy metals (Pb, Cd, and As) were detected in the fruiting bodies harvested from different substrate combinations. These results demonstrated that mixtures of CC with herb residues might be utilized as a novel, practical, and easily available substrate for the cultivation of , which is beneficial for the effective management of herb residues.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/12298093.2018.1454014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037074PMC
March 2018

Identification of a novel promoter from banana aquaporin family gene (MaTIP1;2) which responses to drought and salt-stress in transgenic Arabidopsis thaliana.

Plant Physiol Biochem 2018 Jul 26;128:163-169. Epub 2018 May 26.

Key Laboratory of Genetic Improvement of Bananas, Hainan Province, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China. Electronic address:

Drought and salt stresses often affect plant growth and crop yields. Identification of promoters involved in drought and salt stress responses is of great significance for genetic improvement of crop resistance. Our previous studies showed that aquaporin can respond to drought and salt stresses, but its promoter has not yet been reported in plants. In the present study, cis-acting elements of MaAQP family member promoters were systematically analyzed in banana. Expression of MaTIP1; 2 was induced by drought and salt stresses but not sensitive to cold stress, waterlogging stress, or mechanical damage, and its promoter contained five stress-related cis-acting elements. The MaTIP1; 2 promoter (841 bp upstream of translation initiation site) from banana (Musa acuminata L. AAA group cv. Brazilian) was isolated through genome walking polymerase chain reaction, and found to contain a TATA Box, CAAT box, ABRE element, CCGTCC box, CGTCA motif, and TCA element. Transformation of the MaTIP1; 2 promoter into Arabidopsis to assess its function indicated that it responds to both drought and salt stress treatments. These results suggest that MaTIP1; 2 utilization may improve drought and salt stresses resistance of the transgenic plants by promoting banana aquaporin expression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plaphy.2018.05.003DOI Listing
July 2018

Elucidating the Mechanisms of the Tomato ovate Mutation in Regulating Fruit Quality Using Proteomics Analysis.

J Agric Food Chem 2017 Nov 9;65(46):10048-10057. Epub 2017 Nov 9.

Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences , 4 Xueyuan Road, Haikou 571101, China.

The ovate mutation has frequently been used to study changes in fruit shape but not fruit quality. A deterioration in fruit quality associated with the ovate mutation was discovered in this study. To elucidate how ovate influences the quality of fruit, we performed a proteomics analysis of the fruits of the ovate mutant (LA3543) and wild-type ("Ailsa Craig", LA2838A) using tandem mass tag analysis. The results indicated that the ovate mutation significantly influences fruit quality in a number of ways, including by reducing the expression of 1-aminocyclopropane-1-carboxylic acid oxidase 3 (ACO3) in ethylene biosynthesis, improving firmness by reducing the amount of pectinesterase and polygalacturonase, reducing sugar accumulation by downregulating the abundance of mannan endo-1,4-β-mannosidase 4, β-galactosidase, and β-amylase, and reducing the malic acid content by downregulating the accumulation of malic enzymes and malate synthase. These findings could inform future improvements in fruit quality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.7b03656DOI Listing
November 2017

Natural Variation in Banana Varieties Highlights the Role of Melatonin in Postharvest Ripening and Quality.

J Agric Food Chem 2017 Nov 8;65(46):9987-9994. Epub 2017 Nov 8.

Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences , Xueyuan Road 4, Haikou, Hainan 571101, People's Republic of China.

This study aimed to investigate the role of melatonin in postharvest ripening and quality in various banana varieties with contrasting ripening periods. During the postharvest life, endogenous melatonin showed similar performance with ethylene in connection to ripening. In comparison to ethylene, melatonin was more correlated with postharvest banana ripening. Exogenous application of melatonin resulted in a delay of postharvest banana ripening. Moreover, this effect is concentration-dependent, with 200 and 500 μM treatments more effective than the 50 μM treatment. Exogenous melatonin also led to elevated endogenous melatonin content, reduced ethylene production through regulation of the expression of MaACO1 and MaACS1, and delayed sharp changes of quality indices. Taken together, this study highlights that melatonin is an indicator for banana fruit ripening in various varieties, and the repression of ethylene biosynthesis and postharvest ripening by melatonin can be used for biological control of postharvest fruit ripening and quality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.7b03354DOI Listing
November 2017

MuMADS1 and MaOFP1 regulate fruit quality in a tomato ovate mutant.

Plant Biotechnol J 2018 05 2;16(5):989-1001. Epub 2017 Nov 2.

Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.

Fruit ripening and quality are common botanical phenomena that are closely linked and strictly regulated by transcription factors. It was previously discovered that a banana MADS-box protein named MuMADS1 interacted with an ovate family protein named MaOFP1 to regulate banana fruit ripening. To further investigate the role of MuMADS1 and MaOFP1 in the regulation of fruit quality, a combination of genetic transformation and transcriptional characterization was used. The results indicated that the co-expression of MuMADS1 and MaOFP1 in the ovate mutant could compensate for fruit shape and inferior qualities relating to fruit firmness, soluble solids and sugar content. The number of differentially expressed genes (DEGs) was 1395 in WT vs. ovate, with 883 up-regulated and 512 down-regulated genes, while the numbers of DEGs gradually decreased with the transformation of MuMADS1 and MaOFP1 into ovate. 'Starch and sucrose metabolism' constituted the primary metabolic pathway, and the gene numbers in this pathway were obviously different when MuMADS1 and MaOFP1 were integrated into ovate. A series of metabolic genes involved in cell wall biosynthesis were up-regulated in the WT vs. ovate, which probably resulted in the firmer texture and lower sugar contents in the ovate fruit. These results demonstrate that MuMADS1 and MaOFP1 are coregulators of fruit quality, facilitating the dissection of the molecular mechanisms underlying fruit quality formation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.12843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902769PMC
May 2018

The core regulatory network of the abscisic acid pathway in banana: genome-wide identification and expression analyses during development, ripening, and abiotic stress.

BMC Plant Biol 2017 Aug 29;17(1):145. Epub 2017 Aug 29.

Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.

Background: Abscisic acid (ABA) signaling plays a crucial role in developmental and environmental adaptation processes of plants. However, the PYL-PP2C-SnRK2 families that function as the core components of ABA signaling are not well understood in banana.

Results: In the present study, 24 PYL, 87 PP2C, and 11 SnRK2 genes were identified from banana, which was further supported by evolutionary relationships, conserved motif and gene structure analyses. The comprehensive transcriptomic analyses showed that banana PYL-PP2C-SnRK2 genes are involved in tissue development, fruit development and ripening, and response to abiotic stress in two cultivated varieties. Moreover, comparative expression analyses of PYL-PP2C-SnRK2 genes between BaXi Jiao (BX) and Fen Jiao (FJ) revealed that PYL-PP2C-SnRK2-mediated ABA signaling might positively regulate banana fruit ripening and tolerance to cold, salt, and osmotic stresses. Finally, interaction networks and co-expression assays demonstrated that the core components of ABA signaling were more active in FJ than in BX in response to abiotic stress, further supporting the crucial role of the genes in tolerance to abiotic stress in banana.

Conclusions: This study provides new insights into the complicated transcriptional control of PYL-PP2C-SnRK2 genes, improves the understanding of PYL-PP2C-SnRK2-mediated ABA signaling in the regulation of fruit development, ripening, and response to abiotic stress, and identifies some candidate genes for genetic improvement of banana.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12870-017-1093-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576091PMC
August 2017
-->