Publications by authors named "Zhiping Yang"

147 Publications

Palladium-catalyzed asymmetric hydrophosphorylation of alkynes: facile access to -stereogenic phosphinates.

Chem Sci 2020 Jun 29;11(28):7451-7455. Epub 2020 Jun 29.

Shenzhen Grubbs Institute and Department of Chemistry, Southern University of Science and Technology Shenzhen 518055 China

Despite the importance of -chiral organophosphorus compounds in asymmetric catalysis, transition metal-catalyzed methods for accessing -chiral phosphine derivatives are still limited. Herein, a catalytic enantioselective method for the synthesis of -stereogenic alkenylphosphinates is developed through asymmetric hydrophosphorylation of alkynes. This process is demonstrated for a wide range of racemic phosphinates and leads to diverse -stereogenic alkenylphosphinates directly.
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http://dx.doi.org/10.1039/d0sc01049aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159285PMC
June 2020

Thoracic radiotherapy and concurrent almonertinib for unresectable stage III EGFR-mutated non-small-cell lung cancer: a phase 2 study.

BMC Cancer 2021 May 7;21(1):511. Epub 2021 May 7.

Department of Thoracic Oncology, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Affiliated Hangzhou Cancer Hospital, Zhejiang University School of Medicine, Hangzhou, 310002, People's Republic of China.

Background: Concurrent chemo-radiotherapy remains the standard treatment in unresectable stage III non-small-cell lung cancer (NSCLC) patients. Several studies have shown a potential value of concurrent epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) with thoracic radiotherapy in EGFR-mutated population, but a high risk of radiation pneumonitis raised a major concern. This study intends to explore the safety and efficacy of concurrent almonertinib, a new third-generation EGFR-TKI, with radiotherapy in locally advanced EGFR-mutated NSCLC patients.

Methods: Locally advanced NSCLC patients harboring sensitive EGFR mutation will be included in this study. A radiotherapy plan will be made for each patient before treatment, and the lung V20 will be calculated. Patients with lung V20 ≥ 28% were enrolled in induction group (arm A), which almonertinib was given for 2 months followed by concurrent radiotherapy; patients with lung V20 < 28% were enrolled in concurrent group (arm B), which almonertinib was given concurrent with thoracic radiotherapy. The primary endpoint is the incidence of grade ≥ 3 radiation pneumonitis within 6 months post-radiotherapy, and the secondary endpoints are local control rate, progression-free survival, and overall survival.

Discussion: The safety and efficacy of third-generation EGFR-TKI concurrent with thoracic radiotherapy in locally advanced EGFR-mutated NSCLC is still unknown. We propose to conduct this phase 2 study evaluating the safety especially the radiation pneumonitis within 6 months post-radiotherapy. This trial protocol has been approved by the Ethics committee of Hangzhou cancer hospital. The ethics number is HZCH-2020-030.

Trial Registration: clinicaltrials.gov, NCT04636593 . Registered 19 November 2020 - Retrospectively registered.
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http://dx.doi.org/10.1186/s12885-021-08266-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103745PMC
May 2021

Sesamin attenuates carrageenan-induced lung inflammation through upregulation of A20 and TAX1BP1 in rats.

Int Immunopharmacol 2020 Nov 22;88:107009. Epub 2020 Sep 22.

Department of Pharmacy, Zhejiang Hospital, Hangzhou, China. Electronic address:

Sesamin is a major component in lignans of sesame seeds, has been described to possess a lot of biological activity. The main objective of our study was to investigate the inhibitory effect and novel molecular mechanisms of sesamin on carrageenan-induced lung inflammation in rats. Here we showed that sesamin can obviously reduce polymorphonuclear neutrophils infiltration and exudate volume. Further studies exhibited sesamin can inhibit cytokines release, polymorphonuclear neutrophils markers production and the degree of lung tissues injury. Western blot analysis revealed that sesamin can inhibit the TRAF6 expression and NF-κB pathway activation in lung tissue. We found that sesamin can increase the expression of A20 and TAX1BP1 in lung tissues, and the interaction between the two molecules. In conclusion, all these results demonstrated that sesamin can attenuate carrageenan-induced lung inflammation, the mechanisms that may be related to upregulation of the novel target A20 and TAX1BP1 which can negative regulation for NF-κB pathway. Importantly, this is the first evidence showing that TAX1BP1 can be as a novel regulatory target to attenuate the lung inflammation.
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http://dx.doi.org/10.1016/j.intimp.2020.107009DOI Listing
November 2020

Osteoprotegerin interacts with syndecan-1 to promote human endometrial stromal decidualization by decreasing Akt phosphorylation.

Hum Reprod 2020 11;35(11):2439-2453

Department of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China.

Study Question: Does osteoprotegerin (OPG) promote human endometrial stromal decidualization?

Summary Answer: OPG is essential for human endometrial stromal decidualization through its interaction with syndecan-1 to decrease Akt phosphorylation.

What Is Known Already: OPG (a cytokine receptor) levels are significantly increased in the circulation of pregnant women. However, the role and mechanism of OPG in human endometrial stromal cell (ESC) decidualization remain elusive.

Study Design, Size, Duration: We analyzed the endometrial expression of OPG in endometrial tissue samples collected from women with regular menstrual cycles (ranging from 25 to 35 days), and decidual tissue samples collected from woman with normal early pregnancy or recurrent pregnancy loss (RPL) who visited the Department of Gynecology and Obstetrics at a tertiary care center from January to October 2018. None of the subjects had hormonal treatment for at least 3 months prior to the procedure. In total, 16 women with normal early pregnancy and 15 with RPL were selected as subjects for this study. The function of OPG in decidualization was explored in a human endometrial stromal cell (HESC) line and primary cultures of HESCs.

Participants/materials, Setting, Methods: We collected endometrial tissues (by biopsy) from the subjects during their menstrual cycle and decidual tissues from subjects with a normal early pregnancy and those with RPL at the time of dilation and curettage. The control group comprised randomly selected women who underwent termination of an apparently normal early pregnancy. The endometrial OPG expression was analyzed using immunohistochemical staining and quantitative RT-PCR (qRT-PCR). Immunofluorescence staining and western blot, and qRT-PCR were used to explore the mRNA and protein expression, respectively, of OPG in an immortalized HESC line and in primary cultures of HESC during proliferation and decidualization. siRNA-mediated knockdown experiments were performed to examine the function of OPG in HESC proliferation and decidualization. Flow cytometry and the cell proliferation MTS assay were performed to further examine the role of OPG in HESC proliferation. We also analyzed decidual marker gene expression by qRT-PCR to assess the consequences of OPG loss for HESC decidualization. A co-immunoprecipitation (IP) assay was used to determine the potential interaction between the OPG and Syndecan-1. Western blot analysis of the rescue experiments performed using the phosphatidylinositol 3-kinase (PI3K) signaling-specific inhibitor LY294002 was used to investigate the downstream signaling pathways through which OPG could mediate HESC decidualization.

Main Results And The Role Of Chance: OPG was expressed in both the human endometrium and in vitro decidualized ESCs. Knockdown experiments revealed that OPG loss impaired the expression of IGF-binding protein-1 (IGFBP-1) (P < 0.05) and prolactin (PRL) (P < 0.05), two specific markers of decidualization, in HESC undergoing decidualization. We also uncovered that OPG knockdown induced the aberrant activation of Akt (protein kinase B) during HESC decidualization (P < 0.05). The inhibition of Akt activation could rescue the impaired expression of the decidual markers PRL (P < 0.05) and IGFBP-1 (P < 0.05) in response to OPG knockdown. Syndecan-1 was considered a potential receptor candidate, as it was expressed in both the endometrium and in vitro cultured stromal cells. Subsequent co-IP experiments demonstrated the interaction between OPG and Syndecan-1 during decidualization. In addition, Syndecan-1 knockdown not only clearly attenuated the decidualization markers PRL (P < 0.05) and IGFBP-1 (P < 0.05) but also induced the aberrant enhancement of Akt phosphorylation in decidualized cells, consistent with the phenotype of OPG knockdown cells. Finally, we revealed that the transcript and protein expression of both OPG and Syndecan-1 was significantly lower in the decidual samples of women with RPL than in those of women with normal pregnancy (P < 0.05).

Large Scale Data: N/A.

Limitations, Reasons For Caution: In this study, based on a number of approaches, it was demonstrated that OPG mediated the repression of Akt that occurs during human stromal cell decidualization, however, the molecular link between OPG and Akt signaling was not determined, and still requires further exploration.

Wider Implications Of The Findings: OPG is required for decidualization, and a decrease in OPG levels is associated with RPL. These findings provide a new candidate molecule for the diagnosis and potential treatment of RPL.

Study Funding/competing Interest(s): This work was supported in part by the National Natural Science Foundation of China U1605223 (to G.S.), 81701457 (to Y.J.) and 81601349 (to Y.J.). The authors have no conflicts of interest to disclose.
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http://dx.doi.org/10.1093/humrep/deaa233DOI Listing
November 2020

Advances in the discovery of novel biomarkers for cancer: spotlight on protein -glycosylation.

Biomark Med 2020 07;14(11):1031-1045

State Key Laboratory of Cancer Biology & National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 127 West Changle Road, Xi'an 710032, China.

Progress on glycosylation and tumor markers has not been extensively reported. Glycosylation plays an important part in post-translational modification. Previous research on glycosylation-modified biomarkers has lagged behind due to insufficient understanding of glycosylation-related regulations. However, some new methods and ideas illustrated in recent research may provide new inspirations in the field. This article aims to review current advances in revealing relationship between tumors and abnormal -glycosylation and discuss leading-edge applications of -glycosylation in developing novel tumor biomarkers.
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http://dx.doi.org/10.2217/bmm-2020-0185DOI Listing
July 2020

Thermo-Optical Tuning Cascaded Double Ring Sensor with Large Measurement Range.

Sensors (Basel) 2020 Sep 9;20(18). Epub 2020 Sep 9.

Department of Optical Engineering, School of Opto-Electronic Engineering, Changchun University of Science and Technology, Changchun 130022, China.

In this paper, a thermo-optic tuning optical waveguide sensor system based on a cascaded double micro-ring resonator is investigated. The system consists of a micro-ring resonator with the microheater as a reference ring and a micro-ring resonator with removing the upper cladding layers as a sensing ring, combined with a microfluidic control. The refractive index change of the sample is measured by the electric power change of the microheater. The experimental results show that the sensitivity of the thermo-optic tuning is 34.231 W/RIU (refractive index units), and the measurement range is 4.325 × 10 RIU, almost eight times larger than that of the cascaded double micro-ring resonator without thermo-optic tuning for the intensity interrogation.
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http://dx.doi.org/10.3390/s20185149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570838PMC
September 2020

Risk Stratification Based on Chronic Liver Failure Consortium Acute Decompensation Score in Patients With Child-Pugh B Cirrhosis and Acute Variceal Bleeding.

Hepatology 2021 Apr 23;73(4):1478-1493. Epub 2021 Jan 23.

State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.

Background And Aims: Optimal candidates for early transjugular intrahepatic portosystemic shunt (TIPS) in patients with Child-Pugh B cirrhosis and acute variceal bleeding (AVB) remain unclear. This study aimed to test the hypothesis that risk stratification using the Chronic Liver Failure Consortium Acute Decompensation score (CLIF-C ADs) may be useful to identify a subgroup at high risk of mortality or further bleeding that may benefit from early TIPS in patients with Child-Pugh B cirrhosis and AVB.

Approach And Results: We analyzed the pooled individual data from two previous studies of 608 patients with Child-Pugh B cirrhosis and AVB who received standard treatment between 2010 and 2017 in China. The concordance index values of CLIF-C ADs for 6-week and 1-year mortality (0.715 and 0.708) were significantly better than those of active bleeding at endoscopy (0.633 [P < 0.001] and 0.556 [P < 0.001]) and other prognostic models. With X-tile software identifying an optimal cutoff value, patients were categorized as low risk (CLIF-C ADs <48), intermediate risk (CLIF-C ADs 48-56), and high risk (CLIF-C ADs >56), with a 5.6%, 16.8%, and 25.4% risk of 6-week death, respectively. Nevertheless, the performance of CLIF-C ADs for predicting a composite endpoint of 6-week death or further bleeding was not satisfactory (area under the receiver operating characteristics curve [AUC], 0.588). A nomogram incorporating components of CLIF-C ADs and albumin, platelet, active bleeding, and ascites significantly improved the prediction accuracy (AUC, 0.725).

Conclusions: In patients with Child-Pugh B cirrhosis and AVB, risk stratification using CLIF-C ADs identifies a subgroup with high risk of death that may derive survival benefit from early TIPS. With improved prediction accuracy for 6-week death or further bleeding, the data-driven nomogram may help to stratify patients in randomized trials. Future external validation of these findings in patients with different etiologies is required.
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http://dx.doi.org/10.1002/hep.31478DOI Listing
April 2021

Delayed administration of recombinant plasma gelsolin improves survival in a murine model of severe influenza.

F1000Res 2019 6;8:1860. Epub 2019 Nov 6.

Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, 02115, USA.

Host-derived inflammatory responses contribute to the morbidity and mortality of severe influenza, suggesting that immunomodulatory therapy may improve outcomes. The normally circulating protein, human plasma gelsolin, is available in recombinant form (rhu-pGSN) and has beneficial effects in a variety of pre-clinical models of inflammation and injury.   We evaluated delayed therapy with subcutaneous rhu-pGSN initiated 3 to 6 days after intra-nasal viral challenge in a mouse model of influenza A/PR/8/34. Rhu-pGSN administered starting on day 3 or day 6 increased survival (12-day survival: 62 % vs 39 %, pGSN vs vehicle; p < 0.00001, summary of 18 trials), reduced morbidity, and decreased pro-inflammatory gene expression. Rhu-pGSN improves outcomes in a highly lethal influenza model when given after a clinically relevant delay.
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http://dx.doi.org/10.12688/f1000research.21082.2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894358PMC
June 2020

Delayed Administration of Recombinant Plasma Gelsolin Improves Survival in a Murine Model of Penicillin-Susceptible and Penicillin-Resistant Pneumococcal Pneumonia.

J Infect Dis 2019 09;220(9):1498-1502

Department of Environmental Health, Harvard T. H. Chan School of Public Health, Boston, Massachusetts.

Therapy to enhance host immune defenses may improve outcomes in serious infections, especially for antibiotic-resistant pathogens. Recombinant human plasma gelsolin (rhu-pGSN), a normally circulating protein, has beneficial effects in diverse preclinical models of inflammation and injury. We evaluated delayed therapy (24-48 hours after challenge) with rhu-pGSN in a mouse model of pneumococcal pneumonia. rhu-pGSN without antibiotics increased survival and reduced morbidity and weight loss after infection with either penicillin-susceptible or penicillin-resistant pneumococci (serotypes 3 and 14, respectively). rhu-pGSN improves outcomes in a highly lethal pneumococcal pneumonia model when given after a clinically relevant delay, even in the setting of antimicrobial resistance.
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http://dx.doi.org/10.1093/infdis/jiz353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761947PMC
September 2019

Spatiotemporal Distribution of Linear Microcracks and Diffuse Microdamage Following Daily Bouts of Fatigue Loading of Rat Ulnae.

J Orthop Res 2019 10 29;37(10):2112-2121. Epub 2019 Jun 29.

Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China.

Microdamage accumulation contributes to impaired skeletal mechanical integrity. The bone can remove microdamage by initiating targeted bone remodeling. However, the spatiotemporal characteristics of microdamage initiation and propagation and their relationship with bone remodeling in response to fatigue loading, especially for more physiologically relevant daily bouts of compressive loading, remain poorly understood. The right forelimbs of 24 rats were cyclically loaded with a ramp waveform for 1,500 cycles/day, and contralateral ulnae were not loaded as the controls. The rats were divided into four equal groups and loaded for 1, 4, 7, and 10 days, respectively. We demonstrated that linear microcracking accumulation exhibited a non-linear time-varying process within 10 days of loading with peaked microcrack density at Day 7. Disrupted canaliculi surrounding linear microcracks showed high similarity with the temporal changes of linear microcracking accumulation. Observable intracortical resorption regions were found on Day 10. We found more linear microcracks accumulated in the tensile cortex, but longer cracks were observed in the compressive sides. Increased accumulation of diffuse microdamage was observed from Day 4, but no obvious peak was observed within the 10-day loading period. Diffuse damage first initiated in the compressive cortices but extended to tension from Day 7. The diffuse damage exhibited no impacts on the surrounding osteocyte integrity. Together, our findings revealed a time-dependent, bone remodeling-mediated varying process of linear microcracking accumulation following daily bouts of fatigue loading (with observable peak at Day 7 under our loading regime). Our study also identified distinct spatial accumulation of linear and diffuse microdamage in rat ulnae with tensile and compressive strains. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2112-2121, 2019.
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http://dx.doi.org/10.1002/jor.24391DOI Listing
October 2019

Efficacy and safety of sustained-release oxycodone compared with immediate-release morphine for pain titration in cancer patients: A multicenter, open-label, randomized controlled trial (SOCIAL).

Medicine (Baltimore) 2019 Jun;98(24):e15505

Department of Medical Oncology, Hangzhou Cancer Hospital, Hangzhou, China.

Background: The study aims to investigate the effect and safety of sustained-release oxycodone hydrochloride as background dose on pain titration in patients with moderate-to-severe cancer pain.

Material And Methods: Adult patients scheduled with a regular strong opioid for cancer-related pain were recruited and randomly assigned to sustained-release oxycodone group (tablets, 12 hourly) and immediate-release morphine group (5 mg initially, hourly). All patients were hourly reassessed for efficacy and dose titration.

Results: The primary end point was the number of titration cycles required to achieve adequate pain relief (numerical rating scale, NRS ≤ 3). Secondary end points included the proportion of patients achieving adequate pain relief during each cycle, potential predictive factors for titration performance, and side effects. Ninety (94.7%) patients in oxycodone group and 78 (86.7%) patients in morphine group achieved adequate pain control during 1 to 4 cycles of titration. Patients in oxycodone group reached adequate pain control within the first 2 cycles of titration, which was significantly shorter than morphine group wherein the number of titration cycles ranged from 1 to 4 (P = .034). Oxycodone prescription significantly increased the response rate of patients to morphine titration during the first cycle of titration (P = .010). The initial NRS score and oxycodone administration were significantly associated with titration performance. The mild or moderate adverse effects were similar in 2 groups, while severe adverse effects were only identified in morphine group (P = .001).

Conclusion: Use of background sustained-release oxycodone is more efficient and better tolerated on dose titration than immediate-release morphine.
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http://dx.doi.org/10.1097/MD.0000000000015505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587615PMC
June 2019

Early TIPS with covered stents versus standard treatment for acute variceal bleeding in patients with advanced cirrhosis: a randomised controlled trial.

Lancet Gastroenterol Hepatol 2019 08 29;4(8):587-598. Epub 2019 May 29.

State Key Laboratory of Cancer Biology, National Clinical Research Centre for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.

Background: The survival benefit of early placement of transjugular intrahepatic portosystemic shunts (TIPS) in patients with cirrhosis and acute variceal bleeding is controversial. We aimed to assess whether early TIPS improves survival in patients with advanced cirrhosis and acute variceal bleeding.

Methods: We did an investigator-initiated, open-label, randomised controlled trial at an academic hospital in China. Consecutive patients with advanced cirrhosis (Child-Pugh class B or C) and acute variceal bleeding who had been treated with vasoactive drugs plus endoscopic therapy were randomly assigned (2:1) to receive either early TIPS (done within 72 h after initial endoscopy [early TIPS group]) or standard treatment (vasoactive drugs continued to day 5, followed by propranolol plus endoscopic band ligation for the prevention of rebleeding, with TIPS as rescue therapy when needed [control group]). Randomisation was done by web-based randomisation system using a Pocock and Simon's minimisation method with Child-Pugh class (B vs C) and presence or absence of active bleeding as adjustment factors. The primary outcome was transplantation-free survival, analysed in the intention-to-treat population, excluding individuals subsequently found to be ineligible for enrolment. This study is registered with ClinicalTrials.gov, number NCT01370161, and is completed.

Findings: From June 26, 2011, to Sept 30, 2017, 373 patients were screened and 132 patients were randomly assigned to the early TIPS group (n=86) or to the control group (n=46). After exclusion of three individuals subsequently found to be ineligible for enrolment (two patients in the early TIPS group with non-cirrhotic portal hypertension or hepatocellular carcinoma, and one patient in the control group due to non-cirrhotic portal hypertension), 84 patients in the early TIPS group and 45 patients in the control group were included in the intention-to-treat population. 15 (18%) patients in the early TIPS group and 15 (33%) in the control group died; two (2%) patients in the early TIPS group and one (2%) in the control group underwent liver transplantation. Transplantation-free survival was higher in the early TIPS group than in the control group (hazard ratio 0·50, 95% CI 0·25-0·98; p=0·04). Transplantation-free survival at 6 weeks was 99% (95% CI 97-100) in the early TIPS group compared with 84% (75-96; absolute risk difference 15% [95% CI 5-48]; p=0·02) and at 1 year was 86% (79-94) in the early TIPS group versus 73% (62-88) in the control group (absolute risk difference 13% [95% CI 2-28]; p=0·046). There were no significant differences between the two groups in the incidence of hepatic hydrothorax (two [2%] of 84 patients in the early TIPS group vs one [2%] of 45 in the control group; p=0·96), spontaneous bacterial peritonitis (one [1%] vs three [7%]; p=0·12), hepatic encephalopathy (29 [35%] vs 16 [36%]; p=1·00), hepatorenal syndrome (four [5%] vs six [13%]; p=0·10), and hepatocellular carcinoma (four [5%] vs one [2%]; p=0·68). There was no significant difference in the number of patients who experienced other serious adverse events (ten [12%] vs 11 [24%]; p=0·07) or non-serious adverse events (21 [25%] vs 19 [42%]; p=0·05) between groups.

Interpretation: Early TIPS with covered stents improved transplantation-free survival in selected patients with advanced cirrhosis and acute variceal bleeding and should therefore be preferred to the current standard of care.

Funding: National Natural Science Foundation of China, National Key Technology R&D Program, Optimized Overall Project of Shaanxi Province, Boost Program of Xijing Hospital.
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http://dx.doi.org/10.1016/S2468-1253(19)30090-1DOI Listing
August 2019

Interfacial Charging-Decharging Strategy for Efficient and Selective Aerobic NO Oxidation on Oxygen Vacancy.

Environ Sci Technol 2019 06 24;53(12):6964-6971. Epub 2019 May 24.

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Institute of Applied & Environmental Chemistry, College of Chemistry , Central China Normal University , Wuhan 430079 , P. R. China.

Intelligent defect engineering to harness surface molecular processes is at the core of selective oxidation catalysis. Here, we demonstrate that the two-electron-trapped oxygen vacancy (V) of BiOCl, a prototypical F center (V̋''), is a superb site to confine O toward efficient and selective NO oxidation to nitrate. Stimulated by solar light, V̋'' accomplishes NO oxidation through a two-electron charging (V̋'' + O → V̋''-O) and subsequent one-electron decharging process (V̋''-O + NO → V-NO + e). The back-donated electron is retrapped by V to produce a new single-electron-trapped V (V'), simultaneously triggering a second round of NO oxidation (V'-O + NO → V-NO). This unprecedented interfacial charging-decharging scheme alters the peroxide-associated NO oxidation selectivity from NO to NO with a high efficiency and thus hold great promise for the treatment of risky NO species in indoor air.
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http://dx.doi.org/10.1021/acs.est.9b01287DOI Listing
June 2019

Design and improve of the near-infrared phosphor by adjusting the energy levels or constructing new defects.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Aug 26;219:401-410. Epub 2019 Apr 26.

College of Physics Science & Technology, Hebei Key Lab of Optic-Electronic Information and Materials, Hebei University, Baoding 071002, China. Electronic address:

CaGaGeO:Cr phosphors with a broad emission band are prepared by the high temperature solid state method. The emission peak position of CaGaGeO:Cr is located at 745 nm. Considering that the biological detection needs a widely spectra matching with the first biological window (650 nm-900 nm), Al and In are introduced into the Ga sites to tune the peak position. When the Ga (0.62 Å) is substituted by smaller Al (0.535 Å), the crystal field around Cr is enhanced, the energy level T(F) will move up and overlap with E(G) level. As a result, the emission peak of Crshift from 745 nm to 730 nm with an enhancement in the intensity about 19 times due to the electro transfer from E(G) level to T(F) level. However, the energy level T(F) will move down when the Ga is replaced by the larger In (0.8 Å), which leads to the red shift of the emission peak from 745 nm to 780 nm. Meanwhile, the intensity is enhanced about 17 times with constructing the defects. In summary, the wide emission spectra of these samples can be tuned from730nm to 780 nm continuously by controlling the concentration of Al and In.
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http://dx.doi.org/10.1016/j.saa.2019.04.069DOI Listing
August 2019

Defect-Induced Enhancement Emission Intensity of Ca(BO)F(CBF):0.15Bi by Introducing Cation (Na, Sr, Ba) or Anion (Cl).

Inorg Chem 2019 Apr 4;58(8):5356-5365. Epub 2019 Apr 4.

National-Local Joint Engineering Laboratory of New Energy Photoelectric Devices, Hebei Key Laboratory of Optic-electronic Information and Materials, College of Physics Science & Technology , Hebei University , Baoding 071002 , China.

Generally, the emission intensity of phosphors can be enhanced by introducing a proper number of defects. To enhance the emission intensity of Ca(BO)F(CBF):0.15Bi, more Frenkel defects were introduced by Na, Sr, and Ba. It is found that the number of Frenkel defects is related to volume and covalence of the crystal, in which the covalence has a greater effect than the volume. Furthermore, the larger the volume of the crystal is, the stronger the covalence of the crystal is, the more Frenkel defects will be produced. The volume of CaSr (BO)F(CSr BF):0.15Bi is larger than that of CaNa (BO)F(CNa BF):0.15Bi; however, the covalence of Na is similar to that of Sr, which leads to the same trap depth ( E) and defect density (μ) in the quenching concentration. The results also confirmed that the number of Frenkel defects is mainly influenced by the covalence of crystal. Furthermore, crystal distortion also affects the number of Frenkel defects. CSr BF:0.15Bi and CNa BF:0.15Bi have the same distortion at quenching concentration, which results in the same emission intensity in the quenching concentration. CaBa (BO)F (CBa BF):0.15Bi has a larger volume and stronger covalence; meanwhile, it has deeper trap depth ( E) and larger defect density (μ) at the quenching concentration, comparing with CSr BF:0.15Bi and CNa BF:0.15Bi. However, the distortion of CBa BF:0.15Bi is in agreement with CNa BF:0.15Bi and CSr BF:0.15Bi, which leads to the emission intensity of CBa BF:0.15Bi basically the same as that of CNa BF:0.15Bi and CSr BF:0.15Bi in quenching concentration. And the different rates of distortion result in the different quenching concentrations of CNa BF:0.15Bi, CSr BF:0.15Bi, and CBa BF:0.15Bi. Moreover, for CaMg (BO)F(CMg BF):0.15Bi and Ca(BO)FCl (CBFCl ):0.15Bi, there are no Frenkel defects due to weaker covalence and smaller volume of the crystal in CMg BF:0.15Bi. However, Frenkel defects can be observed in CBFCl :0.15Bi due to stronger covalence and larger volume of the crystal, furthermore, and the emission spectra and thermoluminescence spectra of CBFCl :0.15Bi are similar to those of 0.15Bi doped CNa BF:0.15Bi, CSr BF:0.15Bi, and CBa BF:0.15Bi.
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http://dx.doi.org/10.1021/acs.inorgchem.9b00532DOI Listing
April 2019

Environmental Compensation Effect and Synergistic Mechanism of Optimized Nitrogen Management Increasing Nitrogen Use Efficiency in Indica Hybrid Rice.

Front Plant Sci 2019 4;10:245. Epub 2019 Mar 4.

Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Sichuan Agricultural University, Chengdu, China.

Modern rice cultivation relies heavily upon inorganic nitrogen fertilization. Effective fertilizer management is key to sustainable agricultural development. Field and pot trials were conducted in 2014-2016, including a N-labeled urea pot experiment (2014) to investigate mechanism by which optimized nitrogen fertilizer application (OFA) increases nitrogen utilization efficiency (NUE). Results showed that the applied nitrogen recovery efficiencies with OFA were 71.71%, 110.17%, and 51.38% higher than those obtained with traditional nitrogen fertilizer application (TFA) in 2014, 2015, and 2016, respectively. These improvements are attributed mainly to the high recovery efficiency rates derived from spikelet-developing and spikelet-promoting fertilizer applications at the jointing stage and 15-20 d after jointing. Under OFA, the amount of nitrogen fertilizer applied at the early stages was half that used in TFA, which not only promoted the absorption of soil nitrogen, but also reduced nitrogen loss to the environment, as the NUE of basal and tillering fertilizer was only about 22%. Nitrogen applied during the panicle differentiation stage increased the expression of , a NH transporter in roots. This effect significantly improved the uptake of nitrogen derived from fertilizer from jointing to heading stage. Up-regulation of the expression and activity of and at the panicle differentiation and grain-filling stages promoted nitrogen translocation from vegetative organs to reproductive organs. The uptake of nitrogen derived from fertilizer increased from 22.51% in TFA to 35.58% in OFA. Nevertheless, rice absorbs most of the nitrogen it requires from the soil. The OFA treatment could effectively utilize the environmental compensation effect, promote the absorption and transport of nitrogen, and ultimately lead to improvement in NUE. Future research should aim to understand the soil nitrogen supply capacity in order to apply nitrogenous fertilizer in such a way that it sustains the nitrogen balance.
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http://dx.doi.org/10.3389/fpls.2019.00245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410729PMC
March 2019

Pyridoxamine Treatment of HK-2 Human Proximal Tubular Epithelial Cells Reduces Oxidative Stress and the Inhibition of Autophagy Induced by High Glucose Levels.

Med Sci Monit 2019 Feb 25;25:1480-1488. Epub 2019 Feb 25.

Department of Nephrology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, China (mainland).

BACKGROUND Diabetic nephropathy is a predominant cause of renal failure, which is an important chronic complication of diabetes. Pyridoxamine (PM) has been reported to protect renal tubular epithelial cells against oxidative damage and delay or inhibit the development and generation of glucose-induced renal insufficiency at the early stage of disease. In this study, we attempted to explore the protection mechanism of PM on human proximal tubular epithelial cells (HK-2 cells) induced by high glucose. MATERIAL AND METHODS HK-2 cells were cultivated by high glucose medium in the absence or presence of PM. Cell Counting Kit-8 was used to investigate the most appropriate drug concentration of PM by detecting the cell viability of HK-2 cells. The expression of autophagy-related protein Beclin-1, LC-3II, and p62 was measured by western blot analysis, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), and immunofluorescence. The expression and localization of Beclin-1 and p62 were also detected via immunofluorescence. The intracellular reactive oxygen species generation was detected using the reactive oxygen species assay kit. The effects of PM on antioxidant defenses were evaluated with glutathione peroxidase (GPx), manganese superoxide dismutase (MnSOD) activity, and glutathione/glutathione disulfide (GSH/GSSG) ratio. RESULTS High glucose levels were able to upregulate the expression of oxidative stress associated protein and inhibit autophagy‑associated changes verified by western blotting, RT‑qPCR and immunofluorescence. Administration of PM reversed the high glucose‑induced low-expressed Beclin-1 and LC-3II, and overexpressed p62 and intracellular reactive oxygen species levels. Furthermore, non-enzymatic antioxidant defenses and enzymatic antioxidant defenses were turned on by the application of PM. CONCLUSIONS Treatment with PM could reverse high glucose-induced inhibition of autophagy and oxidative stress.
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http://dx.doi.org/10.12659/MSM.914799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400021PMC
February 2019

Reverse effect of Sm on Ce in CaBOCl:Ce/Tb/Sm phosphor: Luminescence, energy transfer and occupation site.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Apr 16;213:141-149. Epub 2019 Jan 16.

College of Physics Science & Technology, Hebei Key Lab of Optic-Electronic Information and Materials, Hebei University, Baoding 071002, China. Electronic address:

A series of CaBOCl:Ce, Tb, Sm are successfully synthesized by a high temperature solid state reaction. And the crystal structure, luminescence property and energy transfer mechanism of Ce/Sm, Tb/Sm and Ce/Tb/Sm in CaBOCl are investigated. There is an interesting phenomenon that the right side of the Ce emission spectra in CaBOCl:Ce, Sm compresses gradually with increasing the Sm concentration. By means of refinement, it can be found that Sm ions are mainly occupied in the Ca sites, because the cell of the Ca lattice is easily occupied. Then, the emission peak2 of Ce in Ca sites occurs to blue shift. While the emission peak1 of Ce in Ca sites is basically stability. However, the emission of Ce in CaBOCl:Ce, Tb, Sm have different changes with increasing the Sm concentration that the emission peaks of Ce shift to long wave at first then to short wave and the FWHM remains stability. This can be attributed to the existence of Tb. For the energy transfer mechanism, according to Dexter's energy transfer theory and Reisfeld's approximation, the energy transfer process of Ce/Sm and Tb/Sm should be the dipole-dipole interaction. And white emitting phosphor is achieved by the efficient energy transfer.
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http://dx.doi.org/10.1016/j.saa.2019.01.038DOI Listing
April 2019

Determination of luminescence and occupy sites of Ce in Zn(BO)(PO) by introducing Ca and Mg ions.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Apr 16;213:134-140. Epub 2019 Jan 16.

College of Physics Science & Technology, Hebei Key Lab of Optic-electronic Information and Materials, Hebei University, Baoding 071002, China. Electronic address:

Series of (Zn, M)(BO)(PO) (M = Ca, Mg):Ce were synthesized by a high temperature solid state method, and the luminescence properties were investigated. Zn(BO)(PO):Ce presents two emission bands, which shows the different changing trends with increasing Ce concentration. When introduced Mg and Ca into Zn(BO)(PO), (Zn, M)(BO)(PO) (M = Ca, Mg):Ce also shows two emission bands because Ce occupies three kinds of Zn sites and transits from 5d energy level to double ground state. Therefore, the two emission bands of Zn(BO)(PO):Ce should be assigned to the different occupancy sites of Ce. Moreover, the selective emission was realized and the emission intensity of Ce was enhanced by the cationic substitution.
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http://dx.doi.org/10.1016/j.saa.2019.01.054DOI Listing
April 2019

Identification of hub genes and outcome in colon cancer based on bioinformatics analysis.

Cancer Manag Res 2019 27;11:323-338. Epub 2018 Dec 27.

State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Air Force Military Medical University, Xi'an, China,

Background: Colon cancer is one of the leading malignant neoplasms worldwide. Until now, the concrete mechanisms of colonic cancerogenesis are largely unknown; identification of driven genes and pathways is, therefore, of great importance for monitoring and conquering this disease. This study aims to explore the potential biomarkers and therapeutic targets for colon cancer treatment.

Methods: The gene expression profile of GSE44076 from Gene Expression Omnibus database, including 98 primary colon cancers and 98 normal distant colon mucosa, was deeply analyzed. GEO2R tool was used to screen the differentially expressed genes (DEGs) between colon cancer tissues and normal samples. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis were performed for screening DEGs using Database for Annotation, Visualization and Integrated Discovery database and Panther database. Moreover, Search Tool for the Retrieval of Interacting Genes, Cytoscape software, and Molecular Complex Detection plug-in were used to visualize the protein-protein interaction of these DEGs.

Results: A total of 497 DEGs were obtained, including 129 upregulated genes mainly enriched in Hippo signaling pathway, Wnt signaling pathway, and cytokine-cytokine receptor interaction and 368 downregulated genes enriched in retinol metabolism, steroid hormone biosynthesis, drug metabolism, and chemical carcinogenesis. Using Molecular Complex Detection software, three important modules were selected from the protein-protein interaction network. Moreover, 20 hub genes with high degree of connectivity were selected, including COL1A1, CXCL5, GNG4, TIMP1, and so on. The Kaplan-Meier analysis for overall survival and correlation analysis were applied among the hub genes.

Conclusion: Taken together, DEGs, especially the hub genes such as COL1A1, might be the driven genes in colon cancer progression. More importantly, they might be the novel biomarkers for diagnosis and guiding therapeutic strategies of colon cance
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http://dx.doi.org/10.2147/CMAR.S173240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312054PMC
December 2018

Agglomeration Effect of Medical Education: Based on the Web of Science Database.

J Transl Int Med 2018 Dec 31;6(4):165-172. Epub 2018 Dec 31.

Air Force Military Medical University, Xi'an, 710032 Shaanxi Province, China.

By retrieving the 1900-2016 medical education-related essays from the web of science database, UCINET software was used to build the national cooperation network and its nested visualization software, and NetDraw was used to visualize the country cooperation networks in different time windows. We found that international medical education papers began to show exponential growth until 1945 and international cooperation did not begin to become dense until 1961. With the increasing number of participating countries in international medical education, the cooperation factions formed more complicated. The intensity of international cooperation between the United States, Britain and other major international medical education powers has been declining from 1991 to 2016. Between Brazil and China, during 1996-2016, the center of cooperation network has been on the rise for a long time, and the intensity of Canada's cooperation in medical education research has been on the rise for nearly 25 years. The center of international medical education is gradually being transferred from the United States to Canada.
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http://dx.doi.org/10.2478/jtim-2018-0027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326029PMC
December 2018

Trap distribution and mechanism for near infrared long-afterglow material AlMgGaO:Cr.

Dalton Trans 2019 Jan;48(2):618-627

College of Physics Science & Technology, Hebei Key Lab of Optic-electronic Information and Materials, Hebei University, Baoding 071002, China.

Near infrared (NIR) long-afterglow materials have attracted much attention due to their high penetration and low destruction in biological tissues. Here, a series of deep red and near infrared materials, AlMgGaO4:xCr3+, were successfully synthesized by a high temperature solid state method. AlMgGaO4 was selected as the host considering its rich antisite defects, which can effectively capture electrons. The emission spectra of AlMgGaO4:xCr3+ range from 680 nm to 1100 nm, which can be nicely decomposed into four Gaussian bands with peaks centered at 706 nm, 723 nm, 916 nm, and 938 nm, respectively. At low temperature (10 K), the emission spectra show there are four emission peaks: a sharp line (peak 1) and broad emission band (peak 2) come from Cr3+ substituting for the regular octahedron [AlO6], and two broad emission bands (peaks 3 and 4) which originate from the spin-allowed transition 4T2(4F) → 4A2(4F) of Cr3+ in the disordered [GaO6] and [MgO6] octahedra, respectively. Remarkably, after removing the excitation source, it exhibited more than 10 hours of afterglow emission which decreased sharply in the first 30 min and then decreased slowly. With an increase in the Cr3+ concentration, the trap depth became shallower due to the generation of the electronic trap centers . The distribution of trap centers and the mechanism of the persistent luminescence have been carefully analyzed and are also discussed.
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http://dx.doi.org/10.1039/c8dt04399jDOI Listing
January 2019

DNA methylation analysis of selected genes for the detection of early-stage lung cancer using circulating cell-free DNA.

Adv Clin Exp Med 2019 03;28(3):355-360

Department of Chest Surgery, The First Affiliated Hospital of Jiaxing University, Zhejiang, China.

Background: Lung cancer is still the deadliest cancer in the world, but early diagnosis cannot be achieved because of the limitations of diagnostic methods. DNA methylation has been proven to be a potentially powerful tool for cancer detection and diagnosis over the past decade.

Objectives: We explored whether free DNA methylation in plasma can be a reliable biomarker for noninvasive lung cancer detection.

Material And Methods: We detected the methylation of 8 genes in plasma-free DNA of patients with pulmonary space-occupying lesions using real-time quantitative methylation-specific polymerase chain reaction (QMSP). Among the 50 selected patients, 39 were confirmed using pathological analysis as having early lung cancer and 11 had an inflammatory pseudotumor.

Results: The QMSP detection showed that the methylation levels of 8 genes in the patients were significantly higher than in the non-lung cancer group. The methylation level of CALCA was the highest and the methylation level of HOXA9 was the lowest. Methylation of RASSF1A, CDKN2A and DLEC1 occured only in lung cancer patients, while methylation of CALCA, CDH13, PITX2, HOXA9, and WT1 occured not only in lung cancer patients, but also in non-lung cancers. The specificity reached 95~100%, whether for a single gene or overall, but the sensitivity was relatively low for each gene. The sensitivity can reach 72% if the methylation of any of the 8 genes is positive and the overall specificity was 91%. The positive and negative predictive values were 96% and 60%, respectively.

Conclusions: Quantitative detection of DNA methylation in plasma is a potential method for early diagnosis of lung cancer.
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http://dx.doi.org/10.17219/acem/84935DOI Listing
March 2019

Tunicamycin specifically aggravates ER stress and overcomes chemoresistance in multidrug-resistant gastric cancer cells by inhibiting N-glycosylation.

J Exp Clin Cancer Res 2018 Nov 9;37(1):272. Epub 2018 Nov 9.

State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 127 West Changle Road, Xi'an, 710032, Shaanxi, China.

Background: Multidrug resistance remains a major obstacle to successful treatment for patients with gastric cancer (GC). Recently, glycosylation has been demonstrated to play a vital role in the acquisition of multidrug resistance. As a potent inhibitor of glycosylation, tunicamycin (Tu) has shown marked antitumor activities in various cancers. In the present study, we attempted to determine the exact effect of Tu on the chemoresistance of GC.

Methods: The cytotoxic effects of drugs on GC cells were evaluated by cell viability assays, and apoptosis was detected by flow cytometry. PCR, western blot analysis, immunofluorescence staining and canonical inhibitors were employed to identify the underlying mechanisms of the specific effects of Tu on multidrug-resistant (MDR) GC cells.

Results: For the first time, we found that MDR GC cells were more sensitive to Tu-induced cell death than the parental cells and that the increased sensitivity might correlate with basal endoplasmic reticulum (ER) stress. In addition, Tu dramatically increased chemotherapy-induced apoptosis by evoking ER stress in GC cells, particularly MDR cells. Further study indicated that these effects were highly dependent on glycosylation inhibition by Tu, rather than its role as a canonical ER stress inducer. Besides, autophagy was markedly triggered by Tu, and blocking autophagy enhanced the combined effects of Tu and chemotherapy on MDR GC cells.

Conclusions: Our results suggest that tumor-targeted glycosylation inhibition may be a feasible strategy to reverse chemoresistance in GC patients.
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http://dx.doi.org/10.1186/s13046-018-0935-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230241PMC
November 2018

Mechanism of Crystal Structure Transformation and Abnormal Reduction in Ca(BO)(PO) F (CBP F): yBi.

Inorg Chem 2018 Nov 12;57(21):13783-13799. Epub 2018 Oct 12.

College of Physics Science & Technology, Hebei Key Laboratory of Optic-Electronic Information and Materials , Hebei University , Baoding 071002 , China.

Tricoordinated planar triangle (PO) may be formed due to the structural differences between planar triangular (BO) and tetrahedral (PO) when (BO) is gradually substituted by (PO). This transformation of structure may affect the luminescence properties of phosphor. Therefore, a series of Ca(BO)(PO) F (CBP F): yBi ( y = 0.05, 0.15; x = 0-3), Ca(PO)(BO) F (CPB F): yBi ( y = 0.05, 0.15; X = 0-1), Ca(PO)F (CPF):0.1Eu, Ca(PO)F (CPF):0.05Bi, and nCaF/CaCl ( n = 0-0.1) are synthesized to explore transformation of the crystal structure on luminescence properties. In CBP F:0.15Bi ( x = 0-3), (PO) is doped to substitute for (BO), the position of emission spectra remains unchanged and the emission intensity decreases rapidly with increasing x. The underlying main reason for that is formation of the triangular plane (PO), which has been verified by performing a series of verification experiments of CPB F: yBi ( y = 0.5, 0.15; X = 0-1). In CPB F: yBi ( y = 0.5, 0.15; X = 0-1), (BO) is doped to substitute for (PO), P-O2 bond breaks and the coordination of (PO) varies from four to three when 0.5 < X < 1; meanwhile, the crystal structure transforms from Ca(PO)F (ICSD-9444) to Ca(PO)F (ISCD-30261), which impedes abnormal reduction from Bi to Bi. Furthermore, Bi should non-luminance in the plane triangular (PO), but luminescence in (BO). Therefore, the emission intensity starts to increase and the emission position suddenly changes from 553 to 474 nm in CPB F: yBi ( y = 0.05, 0.15; 0.5 < X < 1). From this, the crystal structures of CBP F: yBi ( y = 0.05, 0.15; x = 0-3) has been inferred to transform from Ca(BO)F (ISCD-65763) to Ca(PO)F (ISCD-30261), and then to Ca(PO)F (ISCD-9444) with x increasing. Emission position remains unchanged and the emission intensity decreases rapidly in CBP F: yBi ( y = 0.05, 0.15; x = 0-3) do to formation of the triangular plane (PO). In addition, the rate of abnormal reduction from Bi to Bi can be improved by reducing the electronegativity of the environment around the activator or increasing the ionization energy of the activator, which has been confirmed by verification experiments of CPF:0.05Bi, nCaF/CaCl ( n = 0-0.1), and CPF:0.1Eu.
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http://dx.doi.org/10.1021/acs.inorgchem.8b02317DOI Listing
November 2018

Crystal structure, luminescence properties, energy transfer, tunable occupation and thermal properties of a novel color-tunable phosphor NaBaSrBO:xCe,yMn.

Dalton Trans 2018 Oct;47(39):13913-13925

College of Physics Science & Technology, Hebei Key Lab of Optic-Electronic Information and Materials, Hebei University, Baoding 071002, China.

A series of color-tunable NaBa1-zSrzB9O15:Ce3+,Mn2+ phosphors were synthesized by a high temperature solid state method. Luminescence property, energy transfer, thermal stability and cation substitution were investigated in detail. Due to energy transfer, NaBaB9O15:Ce3+,Mn2+ presents violet to green luminescence and manifest a broad excitation range from 200 to 350 nm. The energy transfer mechanism of Ce3+-Mn2+ is identified as a dipole-dipole interaction. NaBa1-zSrzB9O15:Ce3+,Mn2+ displays both Ce3+ violet and Mn2+ green and orange emissions under ultraviolet excitation. It is observed that Sr2+ partial substitution for Ba2+ could adjust the ratio of Mn2+ emission intensity in different cation sites, which results from preferred sites' occupation with modification of the crystal structure. Furthermore, increase in temperature can enhance the energy transfer from Ce3+ to Mn2+, which enhances the Mn2+ emission intensity sharply. The highly thermal-sensitive property of NaBa1-zSrzB9O15:Ce3+,Mn2+ makes it feasible for its potential application in luminescent ratiometric thermometers with wide temperature range.
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http://dx.doi.org/10.1039/c8dt02780cDOI Listing
October 2018

Chrysin attenuates carrageenan-induced pleurisy and lung injury via activation of SIRT1/NRF2 pathway in rats.

Eur J Pharmacol 2018 Oct 14;836:83-88. Epub 2018 Aug 14.

HKBU Institute for Research and Continuing Education, Shenzhen, China; Key Laboratory of Molecular Pharmacology and Drug Evaluation (Ministry of Education of China), School of Pharmacy, Yantai University, Yantai, China. Electronic address:

Chrysin, a natural polyphenol plentifully contained in honey, propolis, vegetables and fruits, it has been reported to exert a variety of pharmacological activities. In the present study, we mainly investigated the protective effects and underlying molecular mechanisms of chrysin on carrageenan-induced lung injury in rats. The results showed that chrysin inhibited the neutrophils infiltration, attenuated histological injury of lung tissues, decreased PMNs markers level and oxidative stress markers levels of lungs in rats. Further studies showed chrysin can inhibit the NF-кB activation in neutrophils cells, activate SIRT1/NRF2 pathway and reduce the expression of adhesion molecule in lung tissues. Taken together, these results suggested that chrysin can attenuate carrageenan-induced lung injury via inhibition of neutrophils activation and oxidative stress response, and that this attenuation is, at least partially, related to up-regulation of SIRT1/NRF2 signaling pathway. This study also validates SIRT1/NRF2 is an effective pharmacological target to protect against carrageenan-induced lung injury.
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http://dx.doi.org/10.1016/j.ejphar.2018.08.015DOI Listing
October 2018

Effect of TGF-β1 on blood CD4CD25 regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis.

Exp Ther Med 2018 Aug 13;16(2):1403-1410. Epub 2018 Jun 13.

Department of Medical Oncology, Jiaxing No. 1 Hospital, Jiaxing, Zhejiang 314001, P.R. China.

Metastatic circulating tumor cells in non-small cell lung cancer (NSCLC) metastasis have been reported to be associated with an immune response. The present study aimed to provide a theoretical basis for the immunomodulatory processes during NSCLC blood metastasis. NSCLC blood and normal peripheral blood mononuclear cells (PBMCs) were collected. The quantity of cluster of differentiation (CD)4CD25 regulatory T (Treg) cells and the intracellular forkhead box protein 3 (Foxp3) expression in CD4CD25 Treg cells were determined by flow cytometry. Furthermore, the effect of transforming growth factor β1 (TGF-β1) on NSCLC blood CD4CD25 Treg cell proliferation was explored by activating blood mononuclear cells with an anti-CD3 monoclonal antibody, interleukin-2 and different doses of TGF-β1. Reverse transcription-quantitative polymerase chain reaction assays were used to detect the mRNA expression of Foxp3. Carboxyfluorescein succinimidyl ester staining was used to analyze the proliferation dynamics of lymphocyte subsets. Results indicate that the proportion of CD4 T cells in the blood of patients with NSCLC was significantly higher compared with normal peripheral blood (P<0.01). Foxp3 expression in NSCLC blood Treg cells was significantly decreased compared with normal peripheral blood (P<0.01). NSCLC blood mononuclear cells treated with TGF-β1 at 1, 5 and 25 ng/ml significantly induced Foxp3 expression in CD4CD25 Treg cells compared with the control group (P<0.05). The proportion of CD4CD25 Treg and CD8 T cells were elevated in generation 6, 7, 8 after 6 days of TGF-β1 treatment compared with untreated cells. The proportion of CD4CD25 Treg and CD8 T cells were elevated in generation 8, 9 and with TGF-β1 treatment after 8 days compared with untreated cells. These results indicate that CD4CD25 Treg cells proliferate at a greater rate compared with CD8 T cells after 4, 6 or 8 days of treatment. The proportion of CD4CD25 Treg cells in NSCLC blood was significantly higher (P<0.05) compared with normal peripheral blood. The number of Foxp3 T cells was significantly lower (P<0.05) compared with normal peripheral blood. The data presented in this study suggest that NSCLC blood CD4CD25 Treg cells are functionally immature and that TGF-β1 may promote maturation.
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http://dx.doi.org/10.3892/etm.2018.6306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090449PMC
August 2018

New opportunities for efficient N fixation by nanosheet photocatalysts.

Nanoscale 2018 Aug;10(33):15429-15435

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Institute of Environmental & Applied Chemistry, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.

Catalytic ammonia synthesis from dinitrogen (N2) under mild conditions has been considered to be the "holy grail" of N2 fixation, which is one of the most important chemical processes in the agriculture, biological and industrial fields. Given that current artificial N2 fixation is still dominated by the energy-intensive Haber-Bosch process, solar N2 fixation represents an encouraging and fascinating route for carbon-free and energy-saving N2 fixation. However, its practical application is seriously hampered by surface sluggish reaction kinetics. In this minireview, we share our perspectives on the use of two-dimensional (2D) nanosheets for the manipulation of photocatalytic N2 fixation. Nanosheet photocatalysts serve as the perfect platform for the engineering of surface active sites, including defects and iron, all of which can not only bolster photon-exciton interaction toward robust charge carriers generation upon light absorption, but also mimic the function schemes of MoFe-cofactor in nitrogenase toward sufficient N2 binding and activation. These merits endowed by nanosheets photocatalysts provide instructive information on exploring the rich nitrogen photochemistry on solid surfaces and offer new opportunities for the design of novel photocatalysts towards efficient N2 fixation.
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http://dx.doi.org/10.1039/c8nr04277bDOI Listing
August 2018

Is water exchange superior to water immersion in detecting adenomas during colonoscopies? Results from a Bayesian network meta-analysis.

Oncotarget 2018 Jul 17;9(55):30679-30693. Epub 2018 Jul 17.

State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.

Aim: Water-assisted colonoscopy (water exchange [WE] and water immersion [WI]) has been shown to improve the adenoma detection rate. However, few studies have compared these two methods head-to-head. Thus, we conducted a network meta-analysis to integrate both direct and indirect evidence comparing the effectiveness of these two procedures.

Method: We searched PubMed, Web of Science, and the Cochrane Central Register of Controlled Trials for original papers and abstracts published up to March 2018. Randomized controlled trials (RCTs) reporting data in accordance with the eligibility criteria were included in this study. We performed a Bayesian random effects network meta-analysis with mixed comparisons.

Results: Twenty-nine studies ( = 11464 patients) including 6 direct and 23 indirect comparisons were included in this network meta-analysis. There was a statistically significant difference in the efficacy of adenoma detection when WE was compared with WI (risk ratio [RR]: 1.2, 95% credible interval [CrI]: 1.1-1.3), air insufflation (AI; RR: 1.3, 95% CrI: 1.1-1.4), and carbon dioxide (CO) insufflation (RR: 1.2, 95% CrI: 1.1-1.5). The different methods were ranked in order from the most to least effective in adenoma detection as follows: WE, WI, AI, and CO. Moreover, although there were no significant differences in pain scores, willingness to repeat, caecal intubation rate, or total procedure time between WI and WE colonoscopy, WE required a longer caecal intubation time than WI.

Conclusion: This network meta-analysis supposes that WE may be superior to WI in detecting adenomas during colonoscopies without affecting other technical features or patient acceptance.
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http://dx.doi.org/10.18632/oncotarget.25504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078142PMC
July 2018