Publications by authors named "Zhiping Liu"

201 Publications

Megarthrus of China. Part 5. The M. calcaratus complex, with description of three new species (Coleoptera: Staphylinidae: Proteininae).

Zootaxa 2021 Aug 12;5020(2):288-306. Epub 2021 Aug 12.

Musum dhistoire naturelle, Case postale 6434 CH-1211 Genve 6, Switzerland. .

Three new species of Megarthrus Curtis from mainland China belonging to the M. calcaratus species complex are described: M. bajie Liu Cuccodoro sp. nov., M. wujing Liu Cuccodoro sp. nov., and M. wukong Liu Cuccodoro sp. nov.. Also pertaining to this lineage of Megarthus, the East Palaearctic M. zerchei Cuccodoro Lbl is reported here to occur in China (new country record). These four species are diagnosed, illustrated, and keyed. Their distribution in mainland China is mapped, and their affinities are briefly discussed.
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http://dx.doi.org/10.11646/zootaxa.5020.2.3DOI Listing
August 2021

Medication Adherence and Associated Factors in Patients With Type 2 Diabetes: A Structural Equation Model.

Front Public Health 2021 4;9:730845. Epub 2021 Nov 4.

Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

The number of patients with type 2 diabetes (T2D) is increasing. Medication treatment is of great importance to stabilize blood glucose. Previous studies have reported that neuroticism, self-efficacy, and social support are factors associated with medication adherence, but few studies have fully investigated the mechanisms between these factors and medication adherence in patients with T2D. To explore the prevalence of medication adherence and the factors associated with medication adherence in patients with T2D. A cross-sectional study consisting of 483 patients with T2D was conducted from July to December 2020. Questionnaires containing sociodemographic and clinical characteristics, the Morisky Medication Adherence Scale-8 (MMAS-8), the neuroticism subscale of the Eysenck Personality Questionnaire-Revised Short Scale (EPQ-RS), the Multidimensional Scale of Perceived Social Support (MSPSS), and the Diabetes Management Self-efficacy Scale (DMSES) were used to collect data. The structural equation model (SEM) was used to test the hypotheses. This study included 305 (63.1%) medication adherence and 178 (36.9%) medication non-adherence patients with T2D. Social support directly influenced medication adherence (β = 0.115, = 0.029) and indirectly influenced medication adherence through self-efficacy (β = 0.044, = 0.016). Self-efficacy directly influenced medication adherence (β = 0.139, = 0.023). Neuroticism indirectly affected medication adherence through social support (β = -0.027, = 0.023) and self-efficacy (β = -0.019, = 0.014). Moreover, there was a sequential mediating effect of social support and self-efficacy on the relationship between neuroticism and medication adherence (β = -0.010, = 0.012). After controlling for age and gender, similar results were obtained. The model fit indices showed a good fit. The medication adherence of patients with T2D needs to be improved. Neuroticism, social support, and self-efficacy had direct or indirect effects on medication adherence in patients with T2D. Healthcare providers should comprehensively develop intervention programs based on neuroticism, social support, and self-efficacy to improve medication adherence in patients with T2D.
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http://dx.doi.org/10.3389/fpubh.2021.730845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599446PMC
November 2021

Endothelial AMPKα1/PRKAA1 exacerbates inflammation in HFD-fed mice.

Br J Pharmacol 2021 Nov 18. Epub 2021 Nov 18.

Vascular Biology Center, Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, GA, USA.

Background And Purpose: Excess nutrient-induced endothelial cell inflammation is a hallmark in high fat diet (HFD)-induced metabolic syndrome. Pharmacological activation of protein kinase AMP-activated alpha 1(PRKAA1)/5'-Adenosine monophosphate-activated protein kinase alpha1 (AMPKα1) shows its beneficial effects in many studies of cardiometabolic disorders. However, AMPKα1, as a major cellular sensor of energy and nutrients in endothelial cells, has not been studied for its physiological role in excess nutrient-induced endothelial cell (EC) inflammation.

Experimental Approach: Wild-type and EC-specific Prkaa1 knockout mice were fed with an HFD. Body weight, fat mass composition, glucose and lipid levels were monitored regularly. Insulin sensitivity was analyzed systemically and in major metabolic organs/tissues. Inflammation status in metabolic organs/tissues were examined with quantitative RT-PCR and flow cytometry. Additionally, metabolic status, inflammation severity and signaling in cultured ECs were assayed with multiple approaches at the molecular level.

Key Results: EC Prkaa1 deficiency unexpectedly alleviated HFD-induced metabolic syndromes including decreased body weight and fat mass, enhanced glucose clearance and insulin sensitivity, and relieved adipose inflammation and hepatic steatosis. Mechanistically, PRKAA1 knockdown in cultured ECs reduced endothelial glycolysis and fatty acid oxidation, decreased the levels of acetyl-coA, and suppressed transcription of inflammatory molecules mediated by ATP citrate lyase (ACLY) and histone acetyltransferase p300.

Conclusions And Implications: This unexpected pro-inflammatory effect of endothelial AMPKα1/PRKAA1 in metabolic context provides additional insight in AMPKα1/PRKAA1 activities, warranting that in-depth study and thoughtful consideration should be applied when AMPKα1/PRKAA1 is used as a therapeutic target in the treatment of metabolic syndrome.
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http://dx.doi.org/10.1111/bph.15742DOI Listing
November 2021

Prediction and prognostic significance of ALOX12B and PACSIN1 expression in gastric cancer by genome-wide RNA expression and methylation analysis.

J Gastrointest Oncol 2021 Oct;12(5):2082-2092

Department of Oncology, Shandong Provincial Third Hospital, Shandong University, Jinan, China.

Background: Stomach adenocarcinoma (STAD) is one of the common gastrointestinal cancers, characterized by late discovery and metastasis. However, research of gene methylation and expression in gastric cancer (GC) metastasis has been quite limited. This study aimed to investigate the altered gene expression patterns between metastasis and non-metastasis samples using high-throughput RNA and methylation profiles from a large number of patients. Another aim was to identify a specific potential metastasis biomarker, with the ability to predict the metastasis possibility and prognosis of patients with STAD.

Methods: In this study, we integrated The Cancer Genome Atlas (TCGA) program STAD datasets, analyzed the RNA expression and DNA methylation data between non-metastasis (M0) and distant metastasis (M1) samples, and evaluated the candidate biomarker in survival and prognosis of GC.

Results: Among all patients enrolled, 329 with M0 and M1 information were positive for RNA analysis, and 353 with M0 and M1 information were positive for methylation analysis. We found 29 upregulated and 200 downregulated genes in RNA level, and 5,046 hypermethylated and 8,563 hypomethylated probes in methylation level. Among these genes, we found high RNA expression level and low DNA methylation level of and in GC metastasis samples. Patients with high expression of these 2 genes had poor overall survival (OS), progression-free survival (PFS), and post-progression survival (PPS).

Conclusions: The expression levels of and were higher in the metastasis than non-metastasis group, and participants with high expression of these 2 genes were found to have poor survival. The genes and are potential biomarkers of metastasis and poor prognosis, especially in early stage GC, and provide additional information for subsequent comprehensive treatment of GC.
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http://dx.doi.org/10.21037/jgo-21-508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576218PMC
October 2021

DHX9 contributes to the malignant phenotypes of colorectal cancer via activating NF-κB signaling pathway.

Cell Mol Life Sci 2021 Dec 13;78(24):8261-8281. Epub 2021 Nov 13.

School of Basic Medicine, Gannan Medical University, Ganzhou, 341000, Jiangxi, China.

Colorectal cancer (CRC) is the leading cause of cancer-related mortality worldwide, which makes it urgent to identify novel therapeutic targets for CRC treatment. In this study, DHX9 was filtered out as the prominent proliferation promoters of CRC by siRNA screening. Moreover, DHX9 was overexpressed in CRC cell lines, clinical CRC tissues and colitis-associated colorectal cancer (CAC) mouse model. The upregulation of DHX9 was positively correlated with poor prognosis in patients with CRC. Through gain- and loss-of function experiments, we found that DHX9 promoted CRC cell proliferation, colony formation, apoptosis resistance, migration and invasion in vitro. Furthermore, a xenograft mouse model and a hepatic metastasis mouse model were utilized to confirm that forced overexpression of DHX9 enhanced CRC outgrowth and metastasis in vivo, while DHX9 ablation produced the opposite effect. Mechanistically, from one aspect, DHX9 enhances p65 phosphorylation, promotes p65 nuclear translocation to facilitate NF-κB-mediated transcriptional activity. From another aspect, DHX9 interacts with p65 and RNA polymerase II (RNA Pol II) to enhance the downstream targets of NF-κB (e.g., Survivin, Snail) expression to potentiate the malignant phenotypes of CRC. Together, our results suggest that DHX9 may be a potential therapeutic target for prevention and treatment of CRC patients.
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http://dx.doi.org/10.1007/s00018-021-04013-3DOI Listing
December 2021

Liver abscess after drug-eluting bead chemoembolization in patients with metastatic hepatic tumors.

Br J Radiol 2021 Nov 29:20211056. Epub 2021 Nov 29.

Department of Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: To investigate the incidence and risk factors for liver abscess formation after treatment with drug-eluting bead chemoembolization (DEB-TACE) in patients with metastatic hepatic tumors (MHT).

Methods: The current study is a retrospective analysis of the clinical data of 137 patients with metastatic hepatic tumors who received DEB-TACE treatment in our institute (Union Hospital, Tongji Medical College, Huazhong University of Science and Technology) between June 2015 and September 2020. Patients were evaluated for the presence or absence of post-DEB-TACE liver abscess. Univariate and multivariate analyses were used to identify risk factors for liver abscess formation.

Results: The incidence of liver abscess formation after the DEB-TACE procedure was 8.76% per patient and 5.53% per procedure. Univariate analysis showed that larger maximum tumor diameter ( = 0.004), Grade 1 artery occlusion ( < 0.001) and systemic chemotherapy within 3 months before the DEB-TACE procedure ( < 0.001) were all associated with liver abscess formation. However, only systemic chemotherapy within 3 months before the DEB-TACE procedure (OR 5.49; 95% CI 0.34-13.54; < 0.001) was identified by multivariate analysis to be an independent risk factor.

Conclusions: Tumor size, Grade 1 artery occlusion and recent systemic chemotherapy may all be associated with increased risk of liver abscess formation following DEB-TACE treatment in patients with metastatic hepatic tumors.

Advances In Knowledge: Identification of risk factors for liver abscess formation following DEB-TACE in patients with MHT. These findings suggest the need for caution and consideration of the aforementioned risk factors on the part of interventional radiologists when designing DEB-TACE strategies and performing post-procedure patient management.
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http://dx.doi.org/10.1259/bjr.20211056DOI Listing
November 2021

The Mediating Effects of Diabetes Distress, Anxiety, and Cognitive Fusion on the Association Between Neuroticism and Fear of Hypoglycemia in Patients With Type 2 Diabetes.

Front Psychol 2021 20;12:697051. Epub 2021 Oct 20.

Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

To explore the relationship between neuroticism and fear of hypoglycemia (FoH) among patients with type 2 diabetes (T2D), as well as the mediating effects of diabetes distress, anxiety, and cognitive fusion on the relationship between neuroticism and FoH. A total of 494 patients with T2D (39.9% females, = 197) were analyzed using the neuroticism scale of the Eysenck Personality Questionnaire-Revised Short Scale (EPQ-RS), the Fear of Hypoglycemia-15 Scale (FH-15), the Diabetes Distress Scale (DDS), the Self-Rating Anxiety Scale (SAS), and the Cognitive Fusion Questionnaire (CFQ). The bootstrapping method was used to test the separate and parallel mediation models. FoH was noted in 17.4% ( = 86) of patients. The correlations between neuroticism, diabetes distress, anxiety, cognitive fusion, and FoH were positive. Diabetes distress, anxiety, and cognitive fusion were significant mediators in the association between neuroticism and FoH in both separate and parallel mediation models. In the parallel mediation model, the mediating effect of anxiety was the highest, and the mediating effect of diabetes distress was the lowest, but no significant differences were found in the comparison of these three indirect effects. This study indicated that neuroticism not only directly affected FoH, but also indirectly influenced FoH the increase of diabetes distress, anxiety, and cognitive fusion in patients with T2D. The results provide a theoretical basis for the development of intervention programs to ameliorate patients' FoH directly and indirectly. Healthcare providers should be encouraged to develop appropriate programs based on improving diabetes distress, anxiety, and cognitive fusion to help patients with T2D improve FoH.
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http://dx.doi.org/10.3389/fpsyg.2021.697051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565853PMC
October 2021

Deficiency of Myeloid Pfkfb3 Protects Mice From Lung Edema and Cardiac Dysfunction in LPS-Induced Endotoxemia.

Front Cardiovasc Med 2021 29;8:745810. Epub 2021 Sep 29.

Department of Cellular Biology and Anatomy, Vascular Biology Center, Medical College of Georgia, Augusta University, Augusta, GA, United States.

Sepsis, a pathology resulting from excessive inflammatory response that leads to multiple organ failure, is a major cause of mortality in intensive care units. Macrophages play an important role in the pathophysiology of sepsis. Accumulating evidence has suggested an upregulated rate of aerobic glycolysis as a key common feature of activated proinflammatory macrophages. Here, we identified a crucial role of myeloid 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (Pfkfb3), a glycolytic activator in lipopolysaccharide (LPS)-induced endotoxemia in mice. Pfkfb3 expression is substantially increased in bone marrow derived macrophages (BMDMs) treated with LPS and in lung macrophages of mice challenged with LPS . Myeloid-specific knockout of in mice protects against LPS-induced lung edema, cardiac dysfunction and hypotension, which were associated with decreased expression of interleukin 1 beta (Il1b), interleukin 6 (Il6) and nitric oxide synthase 2 (Nos2), as well as reduced infiltration of neutrophils and macrophages in lung tissue. Pfkfb3 ablation in cultured macrophages attenuated LPS-induced glycolytic flux, resulting in a decrease in proinflammatory gene expression. Mechanistically, Pfkfb3 ablation or inhibition with a Pfkfb3 inhibitor AZ26 suppresses LPS-induced proinflammatory gene expression via the NF-κB signaling pathway. In summary, our study reveals the critical role of Pfkfb3 in LPS-induced sepsis reprogramming macrophage metabolism and regulating proinflammatory gene expression. Therefore, PFKFB3 is a potential target for the prevention and treatment of inflammatory diseases such as sepsis.
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http://dx.doi.org/10.3389/fcvm.2021.745810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511447PMC
September 2021

Dynamic localization of DNA topoisomerase I and its functional relevance during Drosophila development.

G3 (Bethesda) 2021 09;11(9)

Hengyang College of Medicine, University of South China, Hengyang 421001, China.

DNA topoisomerase I (Top1) maintains chromatin conformation during transcription. While Top1 is not essential in simple eukaryotic organisms such as yeast, it is required for the development of multicellular organisms. In fact, tissue and cell-type-specific functions of Top1 have been suggested in the fruit fly Drosophila. A better understanding of Top1's function in the context of development is important as Top1 inhibitors are among the most widely used anticancer drugs. As a step toward such a better understanding, we studied its localization in live cells of Drosophila. Consistent with prior results, Top1 is highly enriched at the nucleolus in transcriptionally active polyploid cells, and this enrichment responds to perturbation of transcription. In diploid cells, we uncovered evidence for Top1 foci formation at genomic regions not limited to the active rDNA locus, suggestive of novel regulation of Top1 recruitment. In the male germline, Top1 is highly enriched at the paired rDNA loci on sex chromosomes suggesting that it might participate in regulating their segregation during meiosis. Results from RNAi-mediated Top1 knockdown lend support to this hypothesis. Our study has provided one of the most comprehensive descriptions of Top1 localization during animal development.
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http://dx.doi.org/10.1093/g3journal/jkab202DOI Listing
September 2021

Effects of Ocufolin on retinal microvasculature in patients with mild non-proliferative diabetic retinopathy carrying polymorphisms of the MTHFR gene.

BMJ Open Diabetes Res Care 2021 09;9(1)

Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, Florida, USA

Introduction: To evaluate effects of Ocufolin on retinal microvasculature in mild non-proliferative diabetic retinopathy patients who carried methylenetetrahydrofolate reductase (MTHFR) polymorphisms (DR+MTHFRP).

Research Design And Methods: This is a prospective cohort study. Eight DR+MTHFRP (administrated Ocufolin for 6 months) and 15 normal controls (NCs) were recruited. MTHFR polymorphisms were subtyped as normal, C677T, or A1298C. Best-corrected visual acuity (BCVA) was evaluated. Retinal vessel density (VD) and microstructure were evaluated by optical coherence tomography angiography.

Results: BCVA and vascular indices of DR+MTHFRP at baseline were worse than those of NC and improved. Compared with baseline, DR+MTHFRP had significantly improved BCVA during follow-up period (p<0.05). VD of superficial vascular plexus was increased at 4 months (p=0.012), while VD of retinal vascular network did not change (p>0.05). Carriers of A1298C and C677T showed statistically significant increase in VD at all layers by 6 months, while carriers of C677T alone showed no significant change and carriers of A1298C alone showed decreased density from 4 months to 6 months. Microstructure did not change during the follow-up period.

Conclusion: A 6-month intake of Ocufolin is capable of reversing structural changes of microangiopathy in mild non-proliferative DR+MTHFRP. This suggests a novel way to address these impairments prior to catastrophic vision loss.
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http://dx.doi.org/10.1136/bmjdrc-2021-002327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442067PMC
September 2021

of China. Part 4. The complex (Coleoptera, Staphylinidae, Proteininae), with description of a new species from Yunnan Province.

Zookeys 2021 17;1056:17-34. Epub 2021 Aug 17.

Muséum d'histoire naturelle, Case postale 6434 CH-1211, Genève 6, Switzerland Muséum d'histoire naturelle Geneva Switzerland.

The members of the species complex occurring in China, i.e., Bernhauer, Cameron and (Illiger), are diagnosed, and a new species attributed to this informal group, , is described from Yunnan Province. All species are diagnosed and illustrated, and their distribution in mainland China is mapped. The limit of the species complex is refined morphologically.
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http://dx.doi.org/10.3897/zookeys.1056.66553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384827PMC
August 2021

Dock2 affects the host susceptibility to Citrobacter rodentium infection through regulating gut microbiota.

Gut Pathog 2021 Aug 14;13(1):52. Epub 2021 Aug 14.

School of Basic Medicine, Gannan Medical University, Jiangxi, Ganzhou, China.

Background: Dysregulated gut microbiota is one of major pathogenic factors in the development of colitis. Dock2 acts as a guanine nucleotide exchange factor (GEF) and activates small G protein RAC1. Our previous study showed that, compared to wild type (WT) mice, Dock2 mice were more susceptible to colitis induced by Citrobacter rodentium infection. However, it is not clear whether gut microbiota affects the host susceptibility to enteric bacterial infection in Dock2 mice.

Results: In this study, we demonstrated that Dock2 regulated the gut microbiota and affected the host susceptibility to C. rodentium infection by co-housing, fecal microbiota transfer and antibiotic treatment methods. Microbiota analysis by 16 S rRNA gene sequencing showed that Dock2 increased the abundance of prevotellaceae-NK3B31-group and Lactobacillus but decreased that of Helicobacter.

Conclusions: These results suggest that Dock2 regulates the composition of gut microbiota and affects the host susceptibility to C. rodentium infection.
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http://dx.doi.org/10.1186/s13099-021-00449-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364044PMC
August 2021

Effect of EPS and its forms of aerobic granular sludge on sludge aggregation performance during granulation process based on XDLVO theory.

Sci Total Environ 2021 Nov 25;795:148682. Epub 2021 Jun 25.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China; School of Environment and Ecology, Chongqing University, Chongqing 400045, China.

Different forms of extracellular polymeric substances (EPS) play different roles in the formation process of aerobic granular sludge (AGS). This work focused on the contribution of loosely bound EPS (LB-EPS), tightly bound EPS (TB-EPS) and EPS to the aggregation between sludge cells during the start-up of aerobic granular sludge in a sequencing batch reactor. By analyzing the changes of sludge surface characteristics before and after the extraction of each layer of EPS, the contribution of LB-EPS, TB-EPS and EPS to the adhesion and aggregation of sludge cells in the granulation was calculated by surface thermodynamics and the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory. The experimental results showed that AGS reactor basically completed the granulation process and kept stable operation within 40 d. In the process of sludge granulation, the effect of LB-EPS on the aggregation of sludge cells shifted from attraction to repulsion. TB-EPS could improve the surface zeta potential and hydrophobicity and show an attractive effect in the granulation process, which was conducive to the adhesion between sludge cells and was the main contributor to the formation of granules. Additionally, EPS played an apparently positive role in sludge flocculation and could promoted cell aggregation in the whole granulation process.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148682DOI Listing
November 2021

Disruption of endothelial Pfkfb3 ameliorates diet-induced murine insulin resistance.

J Endocrinol 2021 07 14;250(3):93-104. Epub 2021 Jul 14.

Vascular Biology Center, Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.

Overnutrition-induced endothelial inflammation plays a crucial role in high-fat diet (HFD)-induced insulin resistance in animals. Endothelial glycolysis plays a critical role in endothelial inflammation and proliferation, but its role in diet-induced endothelial inflammation and subsequent insulin resistance has not been elucidated. PFKFB3 is a critical glycolytic regulator, and its increased expression has been observed in adipose vascular endothelium of C57BL/6J mice fed with HFD in vivo, and in palmitate (PA)-treated primary human adipose microvascular endothelial cells (HAMECs) in vitro. We generated mice with Pfkfb3 deficiency selective for endothelial cells to examine the effect of endothelial Pfkfb3 in endothelial inflammation in metabolic organs and in the development of HFD-induced insulin resistance. EC Pfkfb3-deficientmice exhibited mitigated HFD-induced insulin resistance, including decreased body weight and fat mass, improved glucose clearance and insulin sensitivity, and alleviated adiposity and hepatic steatosis. Mechanistically, cultured PFKFB3 knockdown HAMECs showed decreased NF-κB activation induced by PA, and consequent suppressed adhesion molecule expression and monocyte adhesion. Taken together, these results demonstrate that increased endothelial PFKFB3 expression promotes diet-induced inflammatory responses and subsequent insulin resistance, suggesting that endothelial metabolic alteration plays an important role in the development of insulin resistance.
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http://dx.doi.org/10.1530/JOE-20-0524DOI Listing
July 2021

KNTC1 knockdown suppresses cell proliferation of colon cancer.

3 Biotech 2021 Jun 11;11(6):262. Epub 2021 May 11.

Department of Oncology, Yijishan Hospital, First Affiliated Hospital of Wannan Medical College, Wuhu, 241000 Anhui China.

Kinetochore-associated protein 1 (KNTC1) is a kind of kinetochore components that ensure the proper functioning of the spindle-assembly checkpoint. To date, the functional information of KNTC1 in colon cancer remains unknown. This study was aimed to investigate the role of KNTC1 in colon cancer. We found KNTC1 was significantly upregulated in the colon cancer compared to the normal tissues. ROC curve showed the area under the curve value of KNTC1 for the prediction of colon cancer was 0.93. Kaplan-Meier revealed highly expressed KNTC1 was associated with poor prognosis. KNTC1 was widely expressed in different colon cancer cell lines. Compared with the control lentiviral infected cells, KNTC1-shRNA cells exhibited significant reduction in cell growth rates and increase in the proportion of cells in the S phase, while decrease in the G1 and G2/M phase. Furthermore, knockdown of KNTC1 dramatically increased apoptosis in the colon cancer cells. Gene set enrichment analysis (GSEA) in gene ontology (GO) showed that KNTC1 is closely associated with cell mitosis-related components, such as nuclear chromatin, centrosome, and spindle. Moreover, upregulated KNTC1 is significantly enriched in the biological process of DNA repair, mRNA processing, microtubule cytoskeleton organization and the molecular function of helicase activity, protein heterodimerization activity and catalytic activity acting on DNA in molecular function. Our data reveal the important roles of KNTC1 in driving tumor progression in colon cancers.
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http://dx.doi.org/10.1007/s13205-021-02800-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113418PMC
June 2021

Decoupled Monitoring Method for Strain and Cracks Based on Multilayer Patch Antenna Sensor.

Sensors (Basel) 2021 Apr 14;21(8). Epub 2021 Apr 14.

School of Logistic Engineering, Wuhan University of Technology, Wuhan 430063, China.

As the rapid development of high-end intelligent equipment continues, the design requirements for crack and strain monitoring equipment are increasing daily. In this paper, a decoupled monitoring method for strain and cracks based on a multilayer patch antenna sensor is studied. First, the monitoring principle for strain and crack decoupling is analyzed. Second, the design method for the multilayer patch antenna sensor is studied, and the hierarchical arrangement, patch size, substrate layer thickness, and feeding line structure are designed on the basis of this method. A quarter-wavelength impedance converter is designed to perform impedance matching and optimize the resonant frequency information. The effects of strain and crack propagation on the resonant frequency of the patch antenna are analyzed through simulations, and the decoupled monitoring method for the structural stress state and crack propagation is discussed. Lastly, the feasibility of decoupled monitoring of strain and cracks is verified experimentally. The results of the theoretical analysis, simulations, and experiments show that the proposed patch antenna sensor based on the multilayer structure can realize decoupled monitoring of strain and cracks in the structure, and the sensor has broad application prospects.
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http://dx.doi.org/10.3390/s21082766DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070952PMC
April 2021

Long-term effects of maize straw return and manure on the microbial community in cinnamon soil in Northern China using 16S rRNA sequencing.

PLoS One 2021 22;16(4):e0249884. Epub 2021 Apr 22.

College of Biological Engineering, Shanxi University, Taiyuan, P. R. China.

Excessive use of chemical fertilizers in agricultural practices have demonstrated a significant impact on microbial diversity and community in soil by altering soil physical and chemical properties, thereby leading to a certain degree of soil salinization and nutritional imbalances. As an organic amendment, maize straw has been widely used to improve soil quality; however, its effect on the soil bacterial community remains limited in Calcarie-Fluvie Cambisols soil in semi-humid arid plateau of North China. In the present experiment, we investigated the effects of continuous straw utilization and fertilization on bacterial communities in Shouyang, Shanxi province, China. Soil samples were collected from 5 different straw utilization and fertilization modes in the following ways: straw mulching (SM), straw crushing (SC), cattle manure (CM), in which way straw is firstly used as silage and then organic fertilizer, control with no straw return (NSR), and control without fertilizers (CK), same amount of N+P fertilizer was applied to the regimes except CK. High-throughput sequencing approaches were applied to the V3-V4 regions of the 16S ribosomal RNA for analysis of the bacterial abundance and community structures. Different long-term straw returning regimes significantly altered the physicochemical properties and bacterial communities of soil, among which CM had the most significant effects on soil fertility and bacterial diversity. Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria, and Gemmatimonadetes were consistently dominant in all soil samples, and Redundancy analysis (RDA) showed significant association of total nitrogen (TN), total phosphorus (TP) and available potassium (AK) with alternation of the bacterial community. Cattle manure had the most beneficial effects on soil fertility and bacterial diversity among different straw utilization and fertilization modes.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249884PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062091PMC
September 2021

Megarthrus of China. Part 3. Comments on the occurrence of Megarthrus nitidulus Kraatz, 1857 in China, and description of a new species, with notes on teratology and phoretic acari in the genus (Coleoptera: Staphylinidae: Proteininae).

Zootaxa 2021 Feb 2;4920(4):zootaxa.4920.4.6. Epub 2021 Feb 2.

Institute of Entomology, College of Plant Protection, Southwest University, Chongqing 400715, China..

A new species, Megarthrus heise Zhang, Cuccodoro, Chen Liu sp. nov., resembling Megarthrus nitidulus Kraatz, 1857 is described from Qinghai, Sichuan, Gansu, Shaanxi, Shanxi provinces and Beijing in China. The record of M. nitidulus in China was apparently based on a misidentification, and the species is thus removed from the list of Chinese Megarthrus. Phoresy of acari and teratological modification of the abdomen are also reported for the first time in the genus.
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http://dx.doi.org/10.11646/zootaxa.4920.4.6DOI Listing
February 2021

The effect of low-level laser therapy on diabetic foot ulcers: A meta-analysis of randomised controlled trials.

Int Wound J 2021 Dec 9;18(6):763-776. Epub 2021 Mar 9.

Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Our purpose was to perform a meta-analysis to evaluate the effect of Low-level laser therapy (LLLT) on diabetic foot ulcers (DFUs). The PubMed, Cochrane, Embase, Web of Science, Chinese BioMedical Literature Database (CBM), China National Knowledge Infrastructure (CNKI), VIP and Wanfang databases were searched systematically up to August 27, 2020. Studies that met the inclusion criteria were included in the analysis. A total of 13 randomised controlled trials (RCTs) and 413 patients were analysed. Compared with the control group, LLLT significantly increased the complete healing rate (risk ratio [RR] = 2.10, 95% confidence interval [CI] 1.56-2.83, P < .00001), reduced the ulcer area (standardised mean difference [SMD] = 3.52, 95% CI 1.65-5.38, P = .0002), and shortened the mean healing time (SMD = -1.40, 95% CI -1.90 to -0.91, P < .00001) of patients with DFUs. The quality of the evidence was very low according to the GRADE system. LLLT is a promising and effective adjuvant treatment to accelerate the healing of DFUs. Further evidence from larger samples and higher quality RCTs is needed to prove the effect of LLLT and to determine the most appropriate parameters for the healing of DFUs.
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http://dx.doi.org/10.1111/iwj.13577DOI Listing
December 2021

Apoptosis inducing properties of 3-biotinylate-6-benzimidazole B-nor-cholesterol analogues.

Steroids 2021 05 12;169:108822. Epub 2021 Mar 12.

Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Key Laboratory of Beibu Gulf Environment Change and Resources Utilization, School of Chemistry and Material, Nanning Normal University, Nanning 530001, PR China. Electronic address:

In this work, a series of Biotin-substituted B-nor-cholesteryl benzimidazole compounds were synthesized. The antiproliferativeactivities of these compounds were evaluated in vitro using a series of human cancer cell lines, including HeLa (cervical cancer), SKOV3 (ovarian cancer), T-47D (thymus gland cancer), MCF-7 (human breast cancer) and HEK293T (normal renal epithelial) cells. These compounds displayed distinct antiproliferative activities against the currently tested cancer cells. The apoptotic properties induced by compound 6d were further investigated. Our results showed that compound 6d could induce the apoptosis of SKOV3 cells, blocking the cell growth in S-phase. Western blotting analyses revealed that compound 6d can induce cell apoptosis via the mitochondria-dependent pathway.
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http://dx.doi.org/10.1016/j.steroids.2021.108822DOI Listing
May 2021

High level expression and biochemical characterization of an alkaline serine protease from Geobacillus stearothermophilus to prepare antihypertensive whey protein hydrolysate.

BMC Biotechnol 2021 03 11;21(1):21. Epub 2021 Mar 11.

Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Beijing, 100083, China.

Background: Proteases are important for hydrolysis of proteins to generate peptides with many bioactivities. Thus, the development of novel proteases with high activities is meaningful to discover bioactive peptides. Because natural isolation from animal, plant and microbial sources is impractical to produce large quantities of proteases, gene cloning and expression of target protease are preferred.

Results: In this study, an alkaline serine protease gene (GsProS8) from Geobacillus stearothermophilus was successfully cloned and expressed in Bacillus subtilis. The recombinant GsProS8 was produced with high protease activity of 3807 U/mL after high cell density fermentation. GsProS8 was then purified through ammonium sulfate precipitation and a two-step chromatographic method to obtain the homogeneous protease. The molecular mass of GsProS8 was estimated to be 27.2 kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and 28.3 kDa by gel filtration. The optimal activity of GsProS8 was found to be pH 8.5 and 50 °C, respectively. The protease exhibited a broad substrate specificity and different kinetic parameters to casein and whey protein. Furthermore, the hydrolysis of whey protein using GsProS8 resulted in a large amount of peptides with high angiotensin-I-converting enzyme (ACE) inhibitory activity (IC of 0.129 mg/mL).

Conclusions: GsProS8 could be a potential candidate for industrial applications, especially the preparation of antihypertensive peptides.
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http://dx.doi.org/10.1186/s12896-021-00678-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953746PMC
March 2021

EZH2-mediated epigenetic suppression of SH3BGRL potently inhibits lung cancer progression.

Biochem Biophys Res Commun 2021 04 22;548:53-59. Epub 2021 Feb 22.

Department of Oncology, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China. Electronic address:

Lung cancer is the leading cause of cancer-related deaths worldwide. Lung cancer stem cells (CSCs) are a small population of cancer cells with self-renewal, therapeutic resistance, and tumor relapse capability. Yet the molecular mechanisms underlying lung CSCs self-renewal remain largely unknown. Here, we report that SH3BGRL were down-regulated in lung cancer tissues comparing with normal lung tissues and SH3BGRL low expression was correlated with the poor clinical outcomes of patients with lung cancer. Moreover, SH3BGRL was also weakly expressed in lung CSCs compared with its corresponding lung cancer cells. We first characterize that EZH2 directly binds to SH3BGRL promoter and transcriptional represses SH3BGRL expression in epigenetic level. Functionally, overexpression of SH3BGRL potently suppresses Lung CSCs self-renewal in vitro. The gain of function study reveals that SH3BGRL acts as a novel tumor suppressor via inhibiting lung cancer cell proliferation and migration as well as Lung CSCs self-renewal in vitro. Collectively, our work demonstrates that SH3BGRL hold potential as a favorable prognostic marker and therapeutic target for patients with lung cancer in future.
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http://dx.doi.org/10.1016/j.bbrc.2021.02.056DOI Listing
April 2021

Template synthesis of ordered mesoporous MgO with superior adsorption for Pb(II) and Cd(II).

Environ Sci Pollut Res Int 2021 Jun 20;28(24):31630-31639. Epub 2021 Feb 20.

Faculy of Materials Metallurgy and Chemistry, Jiangxi University of Science and Technology, Ganzhou, Jiangxi, China.

Ordered mesoporous MgO was synthesized via template method by using magnesium nitrate as a precursor and amphiphilic triblock copolymer Pluronic F127 as a template. The products were characterized by X-ray powder diffraction (XRD) and transmission electron microscopy (TEM), and the Brunauer-Emmett-Teller (BET) method was used to calculate the specific surface areas. The effects of aging time, relative humidity, and magnesium nitrate content on the morphology and textural properties of the products were studied. When the aging time was 36 h and the relative humidity was 40%, the ordered mesoporous MgO with uniform pore sizes (3.2 nm), high specific surface areas (517.2 m/g), and high pore volumes (0.42 cm/g) were obtained. Furthermore, the adsorption properties of ordered mesoporous MgO as adsorbent for removal of Pb(II) and Cd(II) ions were studied. The adsorption kinetics and isotherm data agreed well with pseudo-second-order model and Langmuir model, indicating that the adsorption of heavy metal ions on the ordered mesoporous MgO was mainly chemical and homogeneous adsorption. The maximum adsorption capacities for Pb(II) and Cd(II) ions were up to 3073.5 mg/g and 1485.1 mg/g, respectively.
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http://dx.doi.org/10.1007/s11356-021-12797-xDOI Listing
June 2021

RETINAL TISSUE PERFUSION REDUCTION BEST DISCRIMINATES EARLY STAGE DIABETIC RETINOPATHY IN PATIENTS WITH TYPE 2 DIABETES MELLITUS.

Retina 2021 Mar;41(3):546-554

Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida; and.

Purpose: To determine retinal microcirculation measured as retinal tissue perfusion (RTP) in patients with type 2 diabetes mellitus and mild nonproliferative diabetic retinopathy, and to compare its discrimination ability to that of retinal microvasculature and microstructure.

Methods: Thirty eyes of 18 patients with mild nonproliferative diabetic retinopathy and 20 eyes of 20 age-matched and gender-matched normal controls were imaged. Retinal blood flow velocity and flow rate were measured using a retinal function imager. Retinal vessel density (Dbox) and intraretinal layer thicknesses were measured using optical coherence tomography angiography. Retinal tissue perfusion was measured as retinal blood flow divided by the volume of the inner retina.

Results: Compared with normal control, RTP, vessel density, and the thickness of the retinal nerve fiber layer in patients with mild nonproliferative diabetic retinopathy showed significant reduction (P < 0.05). Retinal tissue perfusion had the best discrimination power (area under the curve = 0.97), with a sensitivity of 93.3% and specificity of 85.0%. In the eyes with mild nonproliferative diabetic retinopathy, RTP correlated with arteriolar blood flow velocity (r = 0.403, P = 0.027) but was unrelated to vessel density in any layer (r = -0.010 to 0.261, P > 0.05).

Conclusion: Because of its highest discrimination power, RTP may become a promising biomarker for detecting early-stage diabetic retinopathy.
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http://dx.doi.org/10.1097/IAE.0000000000002880DOI Listing
March 2021

Safety of Ertugliflozin in Patients with Type 2 Diabetes Mellitus Inadequately Controlled with Conventional Therapy at Different Periods: A Meta-Analysis of Randomized Controlled Trials.

J Diabetes Res 2020 14;2020:9704659. Epub 2020 Dec 14.

Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

Aims: To assess the safety of ertugliflozin in patients with type 2 diabetes mellitus (T2DM) inadequately controlled with conventional therapy at different periods.

Methods: We searched PubMed, Embase, and The Cochrane Library from inception to September 23, 2020. A total of six studies involving 4120 patients were included.

Results: Compared with the control group, 15 mg and 5 mg of ertugliflozin were associated with higher risks of genital mycotic infections (GMIs) at 26 weeks ( < 0.0001 and < 0.0001, respectively), 52 weeks ( < 0.00001 and < 0.0001, respectively), and 104 weeks ( < 0.00001 and < 0.0001, respectively). Moreover, females had a higher risk of GMIs than males in the 15 mg group at 26 weeks ( = 0.0008), 52 weeks ( < 0.0001), and 104 weeks ( = 0.02). At 104 weeks, 15 mg and 5 mg of ertugliflozin showed beneficial effects on symptomatic hypoglycemia ( < 0.00001 and = 0.004, respectively) compared with the effects observed in the control group. Compared with the control group, 15 mg and 5 mg of ertugliflozin were associated with higher risks of drug-related adverse events at 26 weeks ( = 0.002 and = 0.002, respectively); 15 mg of ertugliflozin was associated with a higher risk of discontinuation related to adverse events at 104 weeks ( = 0.03). No significant differences were found in the remaining safety outcomes.

Conclusion: This meta-analysis of randomized controlled trials indicates that ertugliflozin is tolerated by T2DM, but the risk of GMIs is noteworthy, especially among females in the high-dose group.
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http://dx.doi.org/10.1155/2020/9704659DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831274PMC
December 2020

A Fully Conjugated 3D Covalent Organic Framework Exhibiting Band-like Transport with Ultrahigh Electron Mobility.

Angew Chem Int Ed Engl 2021 Apr 12;60(17):9321-9325. Epub 2021 Mar 12.

Beijing Advanced Innovation Center for Soft Matter Science and Engineering, State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing, 100029, China.

Although π-conjugated two dimensional (2D) covalent organic frameworks (COFs) have been extensively reported, developing fully π-conjugated 3D COFs is still an extremely difficult problem due to the lack of fully π-conjugated 3D linkers. We synthesize a fully conjugated 3D COF (BUCT-COF-1) by designing a saddle-shaped building block of aldehyde-substituted cyclooctatetrathiophene (COThP)-CHO. As a consequence of the fully conjugated 3D network, BUCT-COF-1 demonstrates ultrahigh Hall electron mobility up to ≈3.0 cm  V  s at room temperature, which is one order of magnitude higher than the current π-conjugated 2D COFs. Temperature-dependent conductivity measurements reveal that the charge carriers in BUCT- COF-1 exhibit the band-like transport mechanism, which is entirely different from the hopping transport phenomena observed in common organic materials. The findings indicate that fully conjugated 3D COFs can achieve electron delocalization and charge-transport pathways within the whole 3D skeleton, which may open up a new frontier in the design of organic semiconducting materials.
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http://dx.doi.org/10.1002/anie.202100464DOI Listing
April 2021

Prkaa1 Metabolically Regulates Monocyte/Macrophage Recruitment and Viability in Diet-Induced Murine Metabolic Disorders.

Front Cell Dev Biol 2020 12;8:611354. Epub 2021 Jan 12.

Vascular Biology Center, Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, GA, United States.

Myeloid cells, including monocytes/macrophages, primarily rely on glucose and lipid metabolism to provide the energy and metabolites needed for their functions and survival. AMP-activated protein kinase (AMPK, its gene is for human, for rodent) is a key metabolic sensor that regulates many metabolic pathways. We studied recruitment and viability of -deficient myeloid cells in mice and the phenotype of these mice in the context of cardio-metabolic diseases. We found that the deficiency of Prkaa1 in myeloid cells downregulated genes for glucose and lipid metabolism, compromised glucose and lipid metabolism of macrophages, and suppressed their recruitment to adipose, liver and arterial vessel walls. The viability of macrophages in the above tissues/organs was also decreased. These cellular alterations resulted in decreases in body weight, insulin resistance, and lipid accumulation in liver of mice fed with a high fat diet, and reduced the size of atherosclerotic lesions of mice fed with a Western diet. Our results indicate that AMPKα1/PRKAA1-regulated metabolism supports monocyte recruitment and macrophage viability, contributing to the development of diet-induced metabolic disorders including diabetes and atherosclerosis.
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http://dx.doi.org/10.3389/fcell.2020.611354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835533PMC
January 2021

Megarthrus of China. Part 2. Megarthrus basicornis Fauvel, 1904 with description of a new species from Mount Emei (Coleoptera: Staphylinidae: Proteininae).

Zootaxa 2020 Dec 14;4895(1):zootaxa.4895.1.7. Epub 2020 Dec 14.

Institute of Entomology, College of Plant Protection, Southwest University, Chongqing 400715, China..

Megarthrus budai Liu and Cuccodoro sp. nov. from Sichuan province in China is described. It is the first record of the genus from Mount Emei, a temperate biodiversity-rich mountain to which this new species seems to be endemic. Megarthrus basicornis Fauvel, 1904, known so far only from India and Nepal, is reported from China for the first time.These two species have many features found mainly in members of the predominantly Holarctic and African M. depressus supergroup of species.
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http://dx.doi.org/10.11646/zootaxa.4895.1.7DOI Listing
December 2020

Mice with a specific deficiency of Pfkfb3 in myeloid cells are protected from hypoxia-induced pulmonary hypertension.

Br J Pharmacol 2021 03 1;178(5):1055-1072. Epub 2021 Feb 1.

Vascular Biology Center, Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.

Background And Purpose: Macrophage infiltration into the lungs is a characteristic of pulmonary hypertension (PH). Glycolysis is the main metabolic pathway for macrophage activation. However, the effect of macrophage glycolysis on the development of PH remains unknown. We investigated the effect of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKBF3), a critical enzyme of macrophage glycolysis, on PH development.

Experimental Approach: Lung tissues from PH patients were examined by immunostaining with macrophage markers. PH was induced in Wistar rats with SU5416/hypoxia and in mice with hypoxia. Lungs and macrophages were isolated for analysis by RT-PCR, western blot, flow cytometry, and immunostaining.

Key Results: Expression of glycolytic molecules was increased in circulating peripheral blood mononuclear cells (PBMCs) and lung macrophages of PH patients. These results were also found in lung macrophages of SU5416/hypoxia (Su/Hx)-induced PH rats and hypoxia-induced PH mice. PH was ameliorated in myeloid-specific Pfkfb3-deficient mice (Pfkfb3 ) or mice treated with the PFKFB3 inhibitor 3PO, compared with their controls. Alveolar macrophages of PH Pfkfb3 mice produced lower levels of growth factors and pro-inflammatory cytokines than those of control mice. Circulating myeloid cells and lung myeloid cells were much fewer in PH Pfkfb3 mice than controls. Mechanistically, overexpression of Hif1a or Hif2a in bone marrow-derived macrophages (BMDMs) cultured with bone marrow of Pfkfb3 mice restored the decreased expression of pro-inflammatory cytokines and growth factors.

Conclusions And Implications: Myeloid Pfkfb3 deficiency protects mice from PH, thereby suggesting that myeloid PFKFB3 is one of the important targets in the therapeutic effect of PFKFB3 inhibition in PH treatment.
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http://dx.doi.org/10.1111/bph.15339DOI Listing
March 2021

SH3GL3 functions as a potent tumor suppressor in lung cancer in a SH3 domain dependent manner.

Biochem Biophys Res Commun 2021 01 7;534:787-794. Epub 2020 Nov 7.

Department of Oncology, The Second Affiliated Hospital, Traditional Chinese Medicine, Shandong University, #1 Jingba Road, Jinan, Shandong, 250031, China. Electronic address:

Lung cancer is one of the most common and aggressive cancer and a leading cause of cancer related deaths worldwide. Few studies have prospectively examined the functions of SH3GL3 in cancer progression, however, the biological functions of SH3GL3 in lung cancer initiation and progression remains largely unknown. Here, we show that both mRNA and protein levels of SH3GL3 are weakly expressed in lung cancer tissues and cell lines and its expression negatively correlates with the survival of lung cancer patients. Overexpression of SH3GL3 dramatically inhibits lung cancer cells malignancy behaviors, including proliferation and migration. Additionally, SH3GL3 curbs cell cycle at G0/G1 phase and induces cellular apoptosis of lung cancer cells and inhibits lung cancer stem cell self-renewal dependents on its SH3 domain. Moreover, we report for the first time that SH3GL3 inhibits lung cancer progression partially through p21 and up-regulates p21 in transcriptional level. Collectively, our work suggests that SH3GL3 acts as a potent tumor suppressor in lung cancer progression and have a potential to serve as diagnostic and prognostic biomarker of lung cancer.
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http://dx.doi.org/10.1016/j.bbrc.2020.10.107DOI Listing
January 2021
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