Publications by authors named "Zhipeng Yu"

94 Publications

Real-time imaging mitochondrial viscosity dynamic during mitophagy mediated by photodynamic therapy.

Anal Chim Acta 2021 Sep 12;1178:338847. Epub 2021 Jul 12.

College of Chemistry and Chemical Engineering, Institutes of Physical Science and Information Technology, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Anhui University) Ministry of Education, Hefei, 230601, PR China. Electronic address:

Photodynamic therapy has been generally developed and approved as a promising theranostic technique in recent years, which requires photosensitizers to bear high efficiency of reactive oxygen species production, precisely targeting ability and excellent biocompatibility. The real-time monitoring the microenvironments such as viscosity dynamic involved in mitophagy mediated by photodynamic therapy is significantly important to understand therapeutic process but barely reported. In this work, a pyridinium-functionalized triphenylamine derivative, (E)-4-(2-(4'-(diphenylamino)-[1,1'-biphenyl]-4-yl)vinyl)-1-methylpyridin-1-ium iodide (Mito-I), was exploited as photosensitizer for mitochondria-targeted photodynamic therapy and as fluorescent probe for imaging the mitochondrial viscosity dynamic during mitophagy simultaneously. The results indicated that the additional phenyl ring in Mito-I was beneficial to promote its efficiency of singlet oxygen production. The excellent capability of targeting mitochondria and singlet oxygen generation allowed Mito-I for the specifically mitochondria-targeted photodynamic therapy. Moreover, Mito-I displayed off-on fluorescence response to viscosity with high selectivity and sensitivity. The observed enhancement in fluorescence intensity of Mito-I revealed the increasingly mitochondrial viscosity during mitophagy mediated by the photodynamic therapy of Mito-I. As a result, this work presents a rare example to realize the mitochondria-targeting photodynamic therapy as well as the real-time monitoring viscosity dynamic during mitophagy, which is of great importance for the basic medical research involved in photodynamic therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aca.2021.338847DOI Listing
September 2021

Self-Monitoring the Endo-Lysosomal Escape and Near-Infrared-Activated Mitophagy To Guide Synergistic Type-I Photodynamic and Photothermal Therapy.

Anal Chem 2021 09 25;93(35):12059-12066. Epub 2021 Aug 25.

Hefei National Laboratory for Physical Science at Microscale, iChem, University of Science and Technology of China, Hefei 230051, P.R. China.

Considering the multiple biological barriers before the entry of photosensitizers (PSs) into cytoplasm, it is of paramount importance to track PSs to elucidate their behaviors and distributions to guide the photodynamic therapy (PDT). Also, the developed PSs suffer from strong oxygen dependency. However, reports on such ideal theranostic platforms are rare. Herein, we developed a theranostic platform () based on the coumarin-based D-π-A system, which, for the first time, can reveal the holistic intracellular delivery pathway and near-infrared (NIR)-activated mitophagy to guide synergistic type-I PDT and photothermal therapy. The dynamic endo-lysosomal escape of was monitored, as well as its changeable distributions in endosomes, lysosomes, and mitochondria, demonstrating the preferential accumulation in mitochondria at the end. Upon NIR-I irradiation, generated toxic radicals and heat, triggering the execution of mitophagy and apoptosis. In vivo experiments on mice indicated that under 808 nm irradiation realized complete cancer ablation, showing great potential for advancements in synergistic phototherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.1c02310DOI Listing
September 2021

Identification of Oncorhynchus mykiss nebulin-derived peptides as bitter taste receptor TAS2R14 blockers by in silico screening and molecular docking.

Food Chem 2021 Aug 12;368:130839. Epub 2021 Aug 12.

Lab of Nutrition and Functional Food, Jilin University, Changchun 130062, PR China.

Human bitter taste receptor TAS2R14 (T2R14) can widely perceive bitterness, which has always been an issue for people to overcome. This study was aimed at identifying bioactive peptides obtained from Oncorhynchus mykiss nebulin hydrolysates as bitter taste receptor blockers by physicochemical property prediction, molecular docking, and in vitro determination of bitterness intensity using electronic tongue. Exploration of the interaction mechanism of these peptides with T2R14 by molecular docking models indicated that peptides ADM and ADW had high affinities for T2R14 to block the binding of bitter substances into the receptor. Addition of ADM and ADW to quinine caused reduction in bitterness intensity, with IC values of 420.32 ± 6.26 μM and 403.29 ± 4.10 μM, respectively. Hydrogen bond interaction and hydrophobic interaction were responsible for manifesting the high affinities of these peptides for the receptor. Residues Thr86, Asp168, and Phe247 may be the key amino acids within the binding site.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.130839DOI Listing
August 2021

Visible-light induced photo-click and release strategy between monoarylsydnone and phenoxylfumarate.

Chem Commun (Camb) 2021 Aug 5;57(66):8135-8138. Epub 2021 Aug 5.

Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China.

We report a visible-light induced photo-click and release platform between monoarylsydnone (MASyd) and phenoxylfumarates. The pyrazoline produced by the cycloaddition undergoes a photo-aromatization to form a fluorescent pyrazole. Meanwhile, the photo-aromatization also serves as the driving force to release fluorophores that are quenched in the form of phenoxylfumarates.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cc02841cDOI Listing
August 2021

Potential mechanisms of the anti-hypertensive effects of RVPSL on spontaneously hypertensive rats using non-targeted serum metabolomics.

Food Funct 2021 Sep 20;12(18):8561-8569. Epub 2021 Sep 20.

College of Food Science and Engineering, Bohai University, Jinzhou 121013, P. R. China.

The study aimed to investigate potential mechanisms for the anti-hypertensive effects of RVPSL on spontaneously hypertensive rats (SHRs) using a non-targeted metabonomic approach. In this study, UPLC/MS-based non-targeted metabolomics was performed to discover metabolite variation of serum in SHRs with RVPSL treatment. As a result, the serum metabolites of SHRs that were administered RVPSL for four weeks exhibited distinct alterations. Nine potential biomarkers, , choline, adenosine, adrenic acid, L-tryptophan, niacinamide, glycocholic acid, propiolic acid, D-glyceraldehyde 3-phosphate, and phosphoglycolic acid, were significantly altered, which were mainly involved in lipid metabolism, vitamin and amino acid metabolism, purine metabolism, the MAPK signaling pathway, and the renin-angiotensin system. This study suggested that RVPSL potentially exerted potent effects of alleviating hypertension in the SHRs mainly integrated regulations of metabolism and production of choline, L-tryptophan, nicotinamide, and adenosine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1fo01546jDOI Listing
September 2021

Solvent-Driven Chirality Switching of a Pillar[4]arene[1]quinone Having a Chiral Amine-Substituted Quinone Subunit.

Front Chem 2021 7;9:713305. Epub 2021 Jul 7.

Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, and Healthy Food Evaluation Research Center, Sichuan University, Chengdu, China.

Several new chiral pillar[4]arene[1]quinone derivatives were synthesized by reacting pillar[4]arene[1]quinone (), containing four 1,4-diethoxybenzene units and one benzoquinone unit, with various chiral amines Michael addition. Due to the direct introduction of chiral substituents on the rim of pillar[n]arene and the close location of the chiral center to the rim of , the newly prepared compounds showed unique chiroptical properties without complicated chiral resolution processes, and unprecedented high anisotropy factor of up to -0.018 at the charge transfer absorption band was observed. Intriguingly, the benzene sidearm attached pillar[4]arene[1]quinone derivative showed solvent- and complexation-driven chirality inversion. This work provides a promising potential for absolute asymmetric synthesis of pillararene-based derivatives.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2021.713305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293272PMC
July 2021

Parameter-free optimization algorithm for iterative wavefront shaping.

Opt Lett 2021 Jun;46(12):2880-2883

Optical focusing through scattering media has a significant impact on optical applications in biological tissues. Recently, iterative wavefront shaping (WFS) has been successfully used to focus light through or inside scattering media, and various heuristic algorithms have been introduced to improve the performance. While these results are encouraging, more efforts are needed to tune parameters towards robust and optimum optimization. Moreover, optimal parameters might differ for different scattering samples and experimental conditions. In this Letter, we propose a "smart" parameter-free algorithm by combining a traditional genetic algorithm with a bat algorithm, and the mutation rate can be automatically calculated through real-time feedback. Using this method in iterative WFS, one can achieve robust and optimum performance without a parameter tuning process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OL.427215DOI Listing
June 2021

A NIR-I light-responsive superoxide radical generator with cancer cell membrane targeting ability for enhanced imaging-guided photodynamic therapy.

Chem Sci 2020 Sep 5;11(37):10279-10286. Epub 2020 Sep 5.

Hefei National Laboratory for Physical Sciences at Microscale, iChem, University of Science and Technology of China Hefei P. R. China.

Photodynamic therapy (PDT), as an emerging treatment modality, which takes advantage of reactive oxygen species (ROS) generated upon light illumination to ablate tumours, has suffered from a limited treatment depth, strong oxygen dependence and short ROS lifespan. Herein, we developed a highly efficient NIR-I light (808 nm laser) initiated theranostic system based on a fluorescent photosensitizer (EBD-1) with cancer cell membrane targeting ability, which can realize large penetration depth in tissue, generate superoxide radicals (O ˙) to relieve the oxygen-dependence, confine the ROS oxidation at the cell membrane, and self-report the cell viability during the PDT process. experiments demonstrated that EBD-1 under 808 nm light successfully accomplished remarkable cancer ablation. This work will be beneficial for the design of novel photosensitizers for PDT-based theranostic systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0sc03093gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162420PMC
September 2020

Spatiotemporal Resolved Live Cell Membrane Tracking through Photo-click Reactions Enriched in Lipid Phase.

Chemistry 2021 Aug 26;27(46):11957-11965. Epub 2021 Jun 26.

Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu, 610064, P. R. China.

A set of photo-switchable monopeptides derived from cis-β-dibenzodiazocine-l-alanine (cis-DBDAA) have been designed and synthesized, which are capable of photo-click reacting with diaryltetrazoles or diarylsydnones in a hydrophobic phospholipid bilayer environment. The DBDAA monopeptides include both a hydrophobic tail on C-terminal, providing high affinity toward lipid membrane, and a modularized functional moiety on N-terminal, enabling rapid optimization of the self-assembly strength to form multifunctional supramolecules. With the cis-DBDAA monopeptides photo-switched into trans-configuration, we were able to disrupt the supramolecular assembly through an efficient photo-click reaction across the lipid bilayer of liposomes. We reveal that the performance of the photo-click reactions between the monopeptides and photo-generated nitrile imine intermediates is significantly enhanced by enrichment of both reactants in the hydrophobic membrane lamel of liposomes. Enrichment of the DBDAA monopeptide in lipid phase serves as a convenient method to introduce bioorthogonal chemical handles on live cell membranes, which enables fluorescence labelling of single cell's membrane with high spatiotemporal resolution to facilitate the studies on cell membrane dynamics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/chem.202101653DOI Listing
August 2021

Atomic Force Microscopy Study of Non-DLVO Interactions between Drops and Bubbles.

Langmuir 2021 Jun 27;37(22):6830-6837. Epub 2021 May 27.

Beijing Key Laboratory of Urban Oil and Gas Distribution Technology, State Key Laboratory of Natural Gas Hydrates, MOE Key Laboratory of Petroleum Engineering, China University of Petroleum, Beijing, No. 18 Fuxue Road, Changping District, 102249 Beijing, P. R. China.

The heterointeraction between liquid drops and air bubbles dispersed in another immiscible liquid is studied with the application of the atomic force microscopy (AFM) probe techniques. The tetradecane drops and air bubbles readily coalescence to form a lens-like structure in 100 mM sodium chloride aqueous solution, demonstrating strong hydrophobic (HB) attraction. The interaction range and strength of this hydrophobic attraction between oil drops and air bubbles is investigated by fine control of electrical double layer thicknesses related to specific electrolyte concentrations, and a midrange term in combination with a short-range term is found to present a proper characterization of this hydrophobic attraction. A further step is taken by introducing a triblock copolymer (Pluronic F68) into the aqueous solution, with results indicating that a relatively long-range steric hindrance (SH) furnished by a polymer "brush" surmounts the hydrophobic attraction. Finally, the interaction between a water drop and an air bubble in tetradecane is also measured as a comparison. The repelling action between a hydrophobic body (air bubble) and water drop indicates a strong repulsion. The present results show an interesting understanding of hydrophobic interactions between drops and bubbles, which is of potential application in controlling dispersion stability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.1c00937DOI Listing
June 2021

Surface EMG-Based Instantaneous Hand Gesture Recognition Using Convolutional Neural Network with the Transfer Learning Method.

Sensors (Basel) 2021 Apr 5;21(7). Epub 2021 Apr 5.

Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.

In recent years, surface electromyography (sEMG)-based human-computer interaction has been developed to improve the quality of life for people. Gesture recognition based on the instantaneous values of sEMG has the advantages of accurate prediction and low latency. However, the low generalization ability of the hand gesture recognition method limits its application to new subjects and new hand gestures, and brings a heavy training burden. For this reason, based on a convolutional neural network, a transfer learning (TL) strategy for instantaneous gesture recognition is proposed to improve the generalization performance of the target network. CapgMyo and NinaPro DB1 are used to evaluate the validity of our proposed strategy. Compared with the non-transfer learning (non-TL) strategy, our proposed strategy improves the average accuracy of new subject and new gesture recognition by 18.7% and 8.74%, respectively, when up to three repeated gestures are employed. The TL strategy reduces the training time by a factor of three. Experiments verify the transferability of spatial features and the validity of the proposed strategy in improving the recognition accuracy of new subjects and new gestures, and reducing the training burden. The proposed TL strategy provides an effective way of improving the generalization ability of the gesture recognition system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s21072540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038633PMC
April 2021

Preparation and identification of antioxidant peptides from cottonseed proteins.

Food Chem 2021 Aug 23;352:129399. Epub 2021 Feb 23.

College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

The objective of this study was to investigate the antioxidant properties of cottonseed peptides. Results indicated that cottonseed peptides prepared by enzymatic hydrolysis and microbial fermentation both showed antioxidant properties. The cottonseed protein enzymatic hydrolysate with molecular weight less than 3 kDa exhibited excellent DPPH, ABTS and hydroxyl radical scavenging activity and ferrous ion chelating activity with EC values of 0.49 ± 0.02, 2.05 ± 0.02, 2.21 ± 0.12, and 0.99 ± 0.03 mg/mL, respectively. Amino acid composition analysis revealed that cottonseed protein hydrolysates are rich in acidic/basic and aromatic amino acids. In addition, among the 19 identified cottonseed protein-derived peptides, YSNQNGRF had the lowest CDOCKER energy and formed hydrogen bonds with Tyr334, Arg380, Arg415, Ser508, and Ser602, and van der Waals interactions with Asn382, Tyr525, Gln530, and Ser555, which all located in the binding site of Keap1-Nrf2 interaction. These findings suggested that the antioxidant peptides from cottonseed protein had the potential as functional ingredients in foods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.129399DOI Listing
August 2021

Straightforward preparation of Na(TiO)SiO hollow nanotubes as anodes for ultralong cycle life lithium ion battery.

Dalton Trans 2021 Feb 1;50(7):2521-2529. Epub 2021 Feb 1.

School of Environmental and Material Engineering, Yantai University, Yantai 264005, China.

One-dimensional Na(TiO)SiO (SNTO) nanotubes have been successfully synthesized by a straightforward hydrothermal method with the assistance of cetyltetramethyl ammonium bromide (CTAB). Herein, the influence of the Si/Ti ratio on the morphology or composition of SNTO hollow nanotubes has been investigated, and the result shows that the optimum molar ratio of the optimal morphology is 1 : 1. The prepared samples were first applied as anodes in lithium ion batteries (LIBs) for the time being and superior rate capability, ultralong and stable cycling lifespan performance were obtained. The facile and uniquely designed one-dimensional SNTO nanotube electrodes delivered a high reversible capacity of 121.9 mA h g after 5000 cycles at a high current of 1.0 A g without significant attenuation. The superior electrochemical properties are attributed to their special nanotube structure with a high specific surface area, which could shorten the ion/electron transport pathway, and increase the number of active sites and the contact area between the electrolyte and active electrodes. Meanwhile, the kinetic analysis of the electrochemical behaviors of SNTO hollow nanotube electrodes was carried out by performing calculations using cyclic voltammograms recorded at different scan rates, and the results showed that the obtained reversible capacity is mainly due to the capacitive contribution. This work expands the types of anode materials for LIBs, which will further promote the development of LIBs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0dt03406aDOI Listing
February 2021

Defective transition metal hydroxide-based nanoagents with hypoxia relief for photothermal-enhanced photodynamic therapy.

J Mater Chem B 2021 01 12;9(4):1018-1029. Epub 2021 Jan 12.

College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Anhui University) Ministry of Education, Hefei, 230601, P. R. China.

Recently, phototherapy has attracted much attention due to its negligible invasiveness, insignificant toxicity and excellent applicability. The construction of a newly proposed nanosystem with synergistic photothermal and photodynamic tumor-eliminating properties requires a delicate structure design. In this work, a novel therapeutic nanoplatform (denoted as BCS-Ce6) based on defective cobalt hydroxide nanosheets was developed, which realized hypoxia-relieved photothermal-enhanced photodynamic therapy against cancer. Defective cobalt hydroxide exhibited high photothermal conversion efficacy at the near-infrared region (49.49% at 808 nm) as well as enhanced catalase-like activity to produce oxygen and greatly boost the singlet oxygen generation by a photosensitizer, Ce6, realizing efficacious dual-modal phototherapy. In vivo and in vitro experiments revealed that BCS-Ce6 can almost completely extinguish implanted tumors in a mouse model and present satisfactory biocompatibility during the treatment. This work sets a new angle of preparing photothermal agents and constructing comprehensive therapeutic nanosystems with the ability to modulate the hypoxic tumor microenvironment for efficient cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0tb02486dDOI Listing
January 2021

Identification of novel umami peptides from myosin via homology modeling and molecular docking.

Food Chem 2021 May 28;344:128728. Epub 2020 Nov 28.

College of Food Science and Engineering, Bohai University, Jinzhou 121013, PR China. Electronic address:

The structure of the umami receptor T1R1/T1R3 was constructed using homology modeling and molecular dynamics, and the interactions between peptides and this umami receptor were studied by molecular docking. The umami intensity of the peptides was also investigated by using an electronic tongue. The results showed that 99.3% of the amino acid residues in the homologous model of the T1R1/T1R3 heterodimer were within the allowable range, which is greater than the threshold requirement of 90% of the residues in the high-quality model structure. Five novel peptides (DK, EEK, EDQK, SEGGR, and QDSIGS) were selected and synthesized. The umami intensity of these five peptides was stronger than that of monosodium glutamate. The docking results revealed that the interactions between peptides and the major amino acids residues Arg151, Asp147, and Gln52 of T1R1 play critical roles in the production of umami taste.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2020.128728DOI Listing
May 2021

Pressure Effect on Order-Disorder Ferroelectric Transition in a Hydrogen-Bonded Metal-Organic Framework.

J Phys Chem Lett 2020 Nov 29;11(22):9566-9571. Epub 2020 Oct 29.

Center for High Pressure Science (CHiPS), State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, China.

Perovskite-like ABX metal-organic frameworks (MOFs) have gathered great interest due to their intriguing chemical and physical properties, including their magnetism, ferroelectricity, and multiferroicity. Pressure is an effective thermal parameter in tuning related properties in MOFs due to the adjustable organic framework. Though spectrum experiments have been made on the structural evolution during decompression, there is a lack of electrical studies on the order-disorder ferroelectric transition in the metal-organic frameworks under pressure. In this work, we use a static pyroelectric current measurement, a dynamic dielectric method combined with a Raman scattering technique with applying pressure, to explore the order-disorder ferroelectric transition in [(CH)NH]Co(HCOO). The ferroelectric transition vanishes around the external pressure of 1.6 GPa, emerging with a new paraelectric phase. Another phase transition was observed at 6.32 GPa, mainly associated with the distortive transition of DMA cations. A phenomenological theory of ferroelectricity vanishing at 1.6 GPa for [(CH)NH]Co(HCOO) is also discussed. Our study gives a comprehensive understanding in the pressure tuning of ferroelectric properties in hybrid inorganic-organic materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jpclett.0c02943DOI Listing
November 2020

Identification of tuna protein-derived peptides as potent SARS-CoV-2 inhibitors via molecular docking and molecular dynamic simulation.

Food Chem 2021 Apr 14;342:128366. Epub 2020 Oct 14.

College of Food Science and Engineering, Bohai University, Jinzhou 121013, PR China.

The present study aimed to identify potential SARS-CoV-2 inhibitory peptides from tuna protein by virtual screening. The molecular docking was performed to elicit the interaction mechanism between targets (M and ACE2) and peptides. As a result, a potential antiviral peptide EEAGGATAAQIEM (E-M) was identified. Molecular docking analysis revealed that E-M could interact with residues Thr190, Thr25, Thr26, Ala191, Leu50, Met165, Gln189, Glu166, His164, His41, Cys145, Gly143, and Asn119 of M via 11 conventional hydrogen bonds, 9 carbon hydrogen bonds, and one alkyl interaction. The formation of hydrogen bonds between peptide E-M and the residues Gly143 and Gln189 of M may play important roles in inhibiting the activity of M. Besides, E-M could bind with the residues His34, Phe28, Thr27, Ala36, Asp355, Glu37, Gln24, Ser19, Tyr83, and Tyr41 of ACE2. Hydrogen bonds and electrostatic interactions may play vital roles in blocking the receptor ACE2 binding with SARS-CoV-2.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2020.128366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553880PMC
April 2021

Xanthine oxidase inhibitory peptides derived from tuna protein: virtual screening, inhibitory activity, and molecular mechanisms.

J Sci Food Agric 2021 Mar 22;101(4):1349-1354. Epub 2020 Sep 22.

Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China.

Background: There has been growing interest in the use of xanthine oxidase (XO) as a therapeutic agent to prevent gout and hyperuricemia. In the present study, XO inhibitory peptides were identified from tuna protein by virtual screening, and molecular docking was used to elicit the interaction mechanism between XO and peptides.

Results: A novel tetrapeptide, EEAK, exhibited high XO inhibitory activity with an IC of 173.00 ± 0.06 μM. Molecular docking analysis revealed that EEAK bound with the pivotal residues of XO's active sites (i.e., Glu802, Arg880, Glu1261) through two conventional hydrogen bond interactions, two attractive charge interactions, and one salt bridge. EEAK could also bind with the residues Phe649, Leu648, Lys771, Ser876, Phe914, and Thr1010 of XO.

Conclusion: This study suggested that conventional hydrogen bond interactions and electrostatic interactions play an important role in XO inhibition. The novel XO inhibitory peptide EEAK from tuna protein could be used as potential candidate for controlling gout and hyperuricemia. © 2020 Society of Chemical Industry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jsfa.10745DOI Listing
March 2021

Identification and molecular docking study of fish roe-derived peptides as potent BACE 1, AChE, and BChE inhibitors.

Food Funct 2020 Jul 13;11(7):6643-6651. Epub 2020 Jul 13.

College of Food Science and Engineering, Bohai University, Jinzhou 121013, P.R. China.

Acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and beta-secretase 1 (BACE 1) play vital roles in the development and progression of Alzheimer's disease (AD). The objective of the present study was to identify fish roe-derived anti-AD peptides with activities against AChE, BChE, and BACE 1. Fish roe proteins were cleaved in silico by gastrointestinal proteases, and the released peptides were collected. Subsequently, the toxicity, solubility, and biological properties of these novel di- and tri-peptides were predicted and validated. Finally, potential anti-AD peptides were docked to targets, i.e., AChE, BChE, and BACE 1. A novel anti-AD tripeptide WIR with potent inhibition of AChE and BACE 1 was identified, with IC values of 43.32 ± 1.22 μM and 2.27 ± 0.35 mM, respectively. In addition, the inhibition rate of WIR (at a concentration of 1.06 ± 0.87 μM) against BChE was 33.5%, and the peptide WIR was able to simultaneously interact with AChE, BChE, and BACE 1. Residues Ser286 of AChE, Asp70 of BChE, and Thr231, Arg235 of BACE 1 played key roles in the interaction with peptide WIR. In summary, peptide WIR exhibits the potential to be an effective treatment for AD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0fo00971gDOI Listing
July 2020

Photoisomerization-enhanced 1,3-dipolar cycloaddition of carbon-bridged octocyclic azobenzene with photo-released nitrile imine for peptide stapling and imaging in live cells.

Org Biomol Chem 2020 08 10;18(29):5602-5607. Epub 2020 Jul 10.

Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China.

A photo-induced 1,3-dipolar cycloaddition between nitrile imine and highly ring-strained N[double bond, length as m-dash]N double bond as a dipolarophile was discovered. The photo-isomerization of carbon-bridged octocyclic azobenzene (CBOA) into its trans-configuration accelerates the ligation reaction at a very rapid rate (28 400 M s). The CBOA-based photo-click reaction was proved to be bioorthogonal. In addition, the NoxaB peptide was successfully cross-linked by a CBOA stapler which plays a dual role: photo-control of the conformation of the peptide and photo-conjugation of probes in live cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0ob01027hDOI Listing
August 2020

Liuwei Dihuang pill suppresses metastasis by regulating the wnt pathway and disrupting -catenin/T cell factor interactions in a murine model of triple-negative breast cancer.

J Tradit Chin Med 2019 12;39(6):826-832

School of Humanities, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.

Objective: To investigate if the Liuwei Dihuang pill (LWDHP) can inhibit metastasis to the liver and lungs in mice bearing triple-negative breast cancer (TNBC), and the molecular mechanism underpinning this action.

Methods: Ninety-nine TNBC bearing-mice were distributed randomly to five groups: control (Con), paclitaxel (PTX), low-dose LWDHP (LLP, 2.3 g·kg-1·d-1), middle-dose LWDHP (MLP, 4.6 g·kg-1·d-1) and high-dose LWDHP (HLP, 9.2 g·kg-1·d-1). The LWDHP were administered (p.o.) to the agonal stage. The morphology of BC cells was observed by hematoxylin & eosin staining. Expression of axin-2, β-catenin, T cell factor (TCF), cyclin- D1 and vascular endothelial growth factor (VEGF) was detected by western blotting or immunofluorescence. β-catenin/TCF-1 interaction was measured using a co-immunoprecipitation assay.

Results: After LWDHP treatment, metastasis of BC cells to the lungs and liver was inhibited, expression of axin-2 was increased, expression of TCF-1, β-catenin, cyclin-D1 and VEGF was decreased, and β-catenin/TCF-1 interaction was disrupted.

Conclusion: The LWDHP could inhibit metastasis of BC cells to the liver and lungs. The molecular mechanism underlying this action may be regulation of protein expression and β-catenin/TCF-1 interactions in the Wnt pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
December 2019

Identification of ovalbumin-derived peptides as multi-target inhibitors of AChE, BChE, and BACE1.

J Sci Food Agric 2020 Apr 17;100(6):2648-2655. Epub 2020 Feb 17.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, P.R. China.

Background: Alzheimer's disease (AD) is a kind of progressive neurodegenerative disease that affects the elderly. There is no ideal treatment for AD. Thus, the purpose of this study is to identify anti-AD peptides from ovalbumin.

Results: The potential tripeptides IEK, LYR, and CIK were selected for molecular docking. The '-CDOCKER_Energy' values of the best docking positions of the tripeptide IEK, LYR, and CIK interacting with acetylcholinesterase (AChE) were 93.8119, 86.9556 and 73.6370 kcal mol , respectively. The '-CDOCKER_Energy' values for interaction with butyrylcholinesterase (BChE) were 96.6386, 80.8392, and 87.4341 kcal mol , respectively. Most importantly, the '-CDOCKER_Energy' values for interaction with β-site amyloid precursor protein cleavage enzyme1 (BACE1) were 85.5903, 71.3342, and 68.4290 kcal mol , respectively. Overall, in vitro assay results demonstrated that the peptide CIK exhibited impressive inhibitory activities against AChE, BChE, and BACE1, with half maximal inhibitory concentration (IC ) values of 6.76, 7.72, and 34.48 μmol L , respectively. In particular, CIK can be joined with some peripheral anion sites (PAS) and catalytic sites on AChE, BChE, and BACE1.

Conclusion: Tripeptide CIK can effectively inhibit the activities of AChE, BChE, and BACE1. Tripeptide CIK therefore has the potential to treat AD effectively. © 2020 Society of Chemical Industry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jsfa.10295DOI Listing
April 2020

Dual Colorimetric/Fluorometric Double-Throw pH-Switches: The Dimroth Rearrangement of N,9-Diaryl 8-Azaadenines.

Chempluschem 2019 04;84(4):427-431

Key Laboratory of Green Chemistry and Technology of Ministry of Education College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu, 610064, P. R. China.

A library of 12N,9-Diaryl 2-methyl-8-azaadenine (DAMA) compounds was designed and constructed through an aryl-pairing combination strategy for identifying a nucleobase-containing molecular switch that functions by the pH-regulated Dimroth rearrangement. By utilizing 2D thin-layer chromatography/mass spectrometry (2D-TLC-MS), the DAMA compounds were easily screened to identify which compounds could be used as molecular switches. The pH-switching ability of the DAMA was achieved by incorporating the acridine group as the key structural unit, as well as dual-modal colorimetric/fluorometric on/off properties as the probe functions. The real-time tracing of the switching process clearly indicated that the paired aromatics on both terminals of the DAMA molecule play a key role in tuning the switching kinetics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cplu.201900117DOI Listing
April 2019

Novel ACE inhibitory tripeptides from ovotransferrin using bioinformatics and peptidomics approaches.

Sci Rep 2019 11 22;9(1):17434. Epub 2019 Nov 22.

Lab of Nutrition and Functional Food, Jilin University, Changchun, 130062, P.R. China.

Food-derived ACE inhibitory peptides have recently attracted increased attention. This work focused on a more efficient in silico method to find ACE inhibitory peptides from ovotransferrin. In this work, ovotransferrin was digested into peptides by virtual enzymolysis. Subsequently, in vitro ACE inhibitory activity of potential tripeptides was conducted following the peptide score, toxicity, and water solubility prediction. Both pharmacophore study and flexible docking were applied to analyze ACE inhibition mechanism of tripeptides. Our results demonstrated that EWL was a potent ACE inhibitory tripeptide with IC value of 380 ± 10 μM. Besides, pharmacophore and flexible docking showed that the pi interaction and hydrogen bond were the key interactions in ACE-EWL complex. It appears that the in vitro ACE inhibitory activity of tripeptide EWL was consistent with its molecular modeling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-019-53964-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874687PMC
November 2019

Brain metastases from lung cancer with neuropsychiatric symptoms as the first symptoms.

Transl Lung Cancer Res 2019 Oct;8(5):682-691

Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.

Background: To investigate the neuropsychiatric symptoms and their treatment and outcomes in lung cancer patients with brain metastases (BM), with an attempt to achieve early detection and prompt management of these symptoms.

Methods: Ten lung cancer patients (8 males and 2 females) with BMs who were treated in our center from 2013 to 2019 were enrolled in this analysis. Without exception, all 10 patients presented with chief complaints of neuropsychiatric symptoms, and BMs were eventually diagnosed. Appropriate treatments were offered, and all patients were followed up.

Results: Two patients died (case 5 died of sudden massive hemoptysis, and case 6 died after his families refused to receive the invasive treatment). Data on 3- and 5-year survival have been obtained from one patient each. The average follow-up duration was 19.4 months (except that two patients were hospitalized only once, and one patient received the second follow-up visit only 9 days after the first visit).

Conclusions: The possibility of BM from lung cancer should be considered when a lung cancer patient develops neuropsychiatric symptoms, and timely diagnosis treatment should be arranged accordingly.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/tlcr.2019.10.02DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835098PMC
October 2019

Interferometry-free noncontact photoacoustic detection method based on speckle correlation change.

Opt Lett 2019 Nov;44(22):5481-5484

Optical speckle patterns occur when a coherent optical wavefront is randomized but such stochastic yet deterministic information about the medium can be decoded. A simple setup is inspired to monitor the decorrelation of speckle patterns within the memory effect range when the medium is photoacoustically perturbated. Experimentally, a linear relationship is confirmed between the speckle correlation change and the peak-to-peak amplitude of the ultrasonic transducer-detected photoacoustic waves, and the detection sensitivity is comparable. Such a plain specklegram-based method may find special interests when no direct contact is allowed between the sample and the photoacoustic detector.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OL.44.005481DOI Listing
November 2019

Novel ACE inhibitors derived from soybean proteins using in silico and in vitro studies.

J Food Biochem 2019 09 5;43(9):e12975. Epub 2019 Jul 5.

Lab of Nutrition and Functional Food, Jilin University, Changchun, P.R. China.

The widespread application of soybean-derived peptides is currently limited due to the challenges in the identification of peptides. In the present work, in silico and in vitro analysis were applied to identify ACE inhibitory tri-peptides from soybean protein. The soybean protein was cleaved by PeptideCutter. Then, unknown tri-peptides were selected to solubility estimation and ADME prediction. Subsequently, Discovery Studio was applied to evaluate the interaction mechanism between ACE and tri-peptides. Finally, in vitro activity of theoretical ACE inhibitory tri-peptides was verified by RP-HPLC method. As a result, DMG was selected as a potent ACE inhibitory peptide. Cell experiment showed that DMG had no cytotoxic effects on HEK-293 cells. And molecular docking results indicated that DMG contacted well with ACE's active sites (Gln281, His353, Ala354, Glu384, Lys511, His513, and Tyr520). Furthermore, DMG could exert potent activity against ACE, with IC value of 3.95 ± 0.11 mM. PRACTICAL APPLICATIONS: Present research showed soybean is a potential protein resource to obtain ACE inhibitory peptides. Simultaneously, virtual screening method is a feasible way to substitute for classical method in emerging nutritional fields. What's more, present study provides a theoretical basis for industrial research on foodstuff for ACE inhibitory peptides without side effects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jfbc.12975DOI Listing
September 2019

Synthesis and evaluation of photo-activatable β-diarylsydnone-l-alanines for fluorogenic photo-click cyclization of peptides.

Org Biomol Chem 2019 07;17(28):6777-6781

Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China.

Herein, we design and synthesize a series of photoactivatable β-diarylsydnone-l-alanines (DASAs), which have excellent photo-reactivity with high fluorescence turn-on toward alkenes in a biocompatible environment. The environmental sensing properties of the resulting fluorescent pyrazoline-alanine facilitate its probing capability. By introducing the DASA residue on the side chain of linear peptides, the macrocyclic peptides resulting from the in situ photo-cyclization toward the alkene residue exhibited fluorogenic translocation through live cell membranes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9ob00898eDOI Listing
July 2019

Photo-accelerated "click" reaction between diarylsydnones and ring-strained alkynes for bioorthogonal ligation.

Chem Commun (Camb) 2019 Jun 5;55(50):7187-7190. Epub 2019 Jun 5.

Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu (610064), China.

We constructed a library of diarylsydnone (DASyd) candidates in search of a photoclickable reaction toward alkynes, enabling an ultra-accelerated reactivity, while suppressing the background cycloaddition in the dark. The in vitro and in vivo protein labelling experiments revealed that the photo-accelerated DASyd-alkyne cycloaddition exhibits robust selectivity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9cc02882jDOI Listing
June 2019

Hydrolysis and Transport of Egg White-Derived Peptides in Caco-2 Cell Monolayers and Everted Rat Sacs.

J Agric Food Chem 2019 May 18;67(17):4839-4848. Epub 2019 Apr 18.

Jilin Provincial Key Laboratory of Nutrition and Functional Food, College of Food Science and Engineering , Jilin University , Changchun , Jilin 130062 , People's Republic of China.

The objective of this paper was to investigate the hydrolysis and transepithelial transport of egg white peptides in Caco-2 cell monolayers and everted rat sacs. Results showed that egg white peptides had higher permeability but lower degradation in Caco-2 cell monolayers than found for everted rat sacs. Peptides LGAKDSTRT, DGSRQPVDN, VNDLQGKTS, and GKKDPVLKD were identified from not only the basolateral (BL) side of Caco-2 cell monolayers but also the serous side of everted rat sacs, suggesting that these four peptides could be transported intact in both model systems. In addition, there were 24 peptides identified from the apical (AP) side of Caco-2 cell monolayers and the mucosal side of everted rat sacs, indicating potential resistance to hydrolysis by brush border membrane peptidases. Among these, peptides IRDLLER, YAEERYP, and IRNVLQPS were demonstrated as having dipeptidyl peptidase IV (DPP-IV) inhibitory activities with IC values of 186.23 ± 15.25, 340.62 ± 4.73, and 598.28 ± 15.12 μM ( P < 0.05), respectively. Furthermore, molecular docking revealed that the DPP-IV inhibitory peptides were predicted to form hydrogen-bonds, π-π bonds, and charge interactions with the activity sites, especially the amino acid residues located in the S2 pocket of DPP-IV, potentially contributing to their DPP-IV inhibitory activities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.9b01904DOI Listing
May 2019
-->