Publications by authors named "Zhipeng Wang"

284 Publications

Desmin Correlated with Cx43 May Facilitate Intercellular Electrical Coupling during Chronic Heart Failure.

Evid Based Complement Alternat Med 2021 3;2021:6621132. Epub 2021 Jul 3.

Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China.

Desmin is one of five major intermediate filament proteins in cardiomyocytes. Desmin contributes to the maintenance of healthy muscle. The desmin content in cardiomyocytes directly affects the long-term prognosis of patients with heart failure, and lack of desmin leads to myocyte contractile dysfunction. However, the mechanism is elusive. In this study, we measured desmin expression using western blotting and qPCR in the failed hearts of human patients and rats. Our results showed that desmin content was reduced at the protein level in failed hearts and isolated cardiomyocytes. The association of desmin and the gap junction proteins connexin 43 (Cx43) and zonula occludens-1 (ZO-1) was also investigated. Immunoprecipitation assay showed that desmin was associated with Cx43 in cardiomyocytes. To compare the electrical integration of skeletal myoblasts in cocultures with cardiac myocytes, familial amyloid polyneuropathy (FAP) activation rate was found in 33% desmin overexpressing skeletal myoblasts. Desmin not only affected Cx43 and ZO-1 expression but also facilitated the complex of Cx43 and ZO-1 in skeletal myoblasts, which enhanced cell-to-cell electrical coupling of skeletal myoblasts with cardiac myocytes. Desmin has potential as a novel therapeutic target for heart failure. Preservation of desmin may attenuate heart failure.
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http://dx.doi.org/10.1155/2021/6621132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275391PMC
July 2021

Chemical screen identifies diverse and novel HDAC inhibitors as repressors of NUT function: implications for NUT carcinoma pathogenesis and treatment.

Mol Cancer Res 2021 Jul 20. Epub 2021 Jul 20.

Department of Pathology, Brigham and Women's Hospital, Harvard Medical School

NUT carcinoma (NC), characterized most commonly by the BRD4-NUTM1 fusion, is a rare, aggressive variant of squamous carcinoma with no effective treatment. BRD4-NUT drives growth and maintains the poorly differentiated state of NC by activating pro-growth genes such as MYC, through the formation of massive, hyperacetylated, super-enhancer-like domains termed megadomains. BRD4-NUT-mediated hyperacetylation of chromatin is facilitated by the chromatin-targeting tandem bromodomains of BRD4, combined with NUT, which recruits the histone acetyltransferase, p300. Here, we developed a high throughput small molecule screen to identify inhibitors of transcriptional activation by NUT. In this dCAS9-based GFP-reporter assay, the strongest hits were diverse histone deacetylase (HDAC) inhibitors. Two structurally unrelated HDAC inhibitors, panobinostat and the novel compound, IRBM6, both repressed growth and induced differentiation of NC cells in proportion to their inhibition of NUT transcriptional activity. These two compounds repressed transcription of megadomain-associated oncogenic genes, such as MYC and SOX2, while upregulating pro-differentiation, non-megadomain-associated genes, including JUN, FOS, and key cell cycle regulators, such as CDKN1A. The transcriptional changes correlate with depletion of BRD4-NUT from megadomains, and redistribution of the p300/CBP-associated chromatin acetylation mark, H3K27ac, away from megadomains towards regular enhancer regions previously populated by H3K27ac. In NC xenograft models, we demonstrated that suppression of tumor growth by panobinostat was comparable to that of bromodomain inhibition, and when combined they improved both survival and growth suppression. Implications: The findings provide mechanistic and pre-clinical rationale for the use of HDAC inhibitors, alone or combined with other agents, in the treatment of NUT carcinoma.
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http://dx.doi.org/10.1158/1541-7786.MCR-21-0259DOI Listing
July 2021

LncRNA-encoded microproteins: A new form of cargo in cell culture-derived and circulating extracellular vesicles.

J Extracell Vesicles 2021 Jul 12;10(9):e12123. Epub 2021 Jul 12.

Laboratory of Protein and Peptide Pharmaceuticals and Laboratory of Proteomics Institute of Biophysics Chinese Academy of Sciences Beijing China.

Advancements in omics-based technologies over the past few years have led to the discovery of numerous biologically relevant peptides encoded by small open reading frames (smORFs) embedded in long noncoding RNA (lncRNA) transcripts (referred to as microproteins here) in a variety of species. However, the mechanisms and modes of action that underlie the roles of microproteins have yet to be fully characterized. Herein, we provide the first experimental evidence of abundant microproteins in extracellular vesicles (EVs) derived from glioma cancer cells, indicating that the EV-mediated transfer of microproteins may represent a novel mechanism for intercellular communication. Intriguingly, when examining human plasma, 48, 11 and 3 microproteins were identified from purified EVs, whole plasma and EV-free plasma, respectively, suggesting that circulating microproteins are primarily enriched in EVs. Most importantly, the preliminary data showed that the expression profile of EV microproteins in glioma patient diverged from the health donors, suggesting that the circulating microproteins in EVs might have potential diagnostic application in identifying patients with glioma.
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http://dx.doi.org/10.1002/jev2.12123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275822PMC
July 2021

A H-Bonding and Electrostatic Interaction Combined Strategy for TcO Separation by a Nitrotriacetate-Derived Amine-Amide Extractant.

Inorg Chem 2021 Jul 14. Epub 2021 Jul 14.

Department of Radiochemistry, China Institute of Atomic Energy, Beijing 102413, P.R. China.

Effective and selective separation of technetium from acidic nuclear liquid waste is highly desirable for partitioning and transmutation but is of significant challenge. Highly efficient extraction of pertechnetate can be achieved by taking H-bonding and electrostatic interaction combined strategy. Base on this strategy, an amine-amide ligand NTAamide(-Oct) was employed to extract TcO in HNO solution. Using -dodecane as a diluent, NTAamide(-Oct) demonstrated excellent extractability and good selectivity toward TcO with a rapid extraction equilibrium that could be reached in less than 1 min. Its maximal loading capacity for TcO was almost 100 times as much as that of traditional amine extractant Aliquat-336 nitrate. Meanwhile, TcO could be efficiently stripped from the loaded organic phase by (NH)CO solution. Slope analysis indicated the formation of a 1:1 complex of NTAamide(-Oct) with TcO. The extraction conformed to the anion exchange extraction model, as confirmed by analyses of single-crystal X-ray diffraction, H NMR titration, FTIR, and ESI-MS.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00111DOI Listing
July 2021

Complexity of the relationship between 2D/3D urban morphology and the land surface temperature: a multiscale perspective.

Environ Sci Pollut Res Int 2021 Jul 8. Epub 2021 Jul 8.

College of Geography and Environment, Shandong Normal University, Jinan, 250358, China.

Urban morphology is a crucial contributor to urban heat island (UHI) effects. However, few studies have explored the complex effect of 2D/3D urban morphology on UHIs from a multiscale perspective. In this study, we chose the central area of Jinan city, which is commonly known as the "furnace," as the case study area. The 2D/3D urban morphology indexes-building coverage ratio (BCR) (for assessing the 2D building density), building volume density (BVD) (for assessing the 3D building density), and frontal area index (FAI) (for assessing 3D ventilation conditions) were calculated and derived to investigate the complexity of the relationship between 2D/3D urban morphology and the land surface temperature (LST) at different scales using the maximum information coefficient (MIC) and geographically weighted regression (GWR). The results indicated that (1) these 2D/3D urban morphology indexes are essential factors that are responsible for LST variation, and BCR is the most important urban morphology index affecting LST, followed by BVD and FAI. Importantly, the relationship between the BCR, BVD, FAI, and LST was an inverse U-shaped curve. (2) The relationship between 2D/3D urban morphology and LST variation showed a significant scale effect. With increased grid size, the correlation between the BCR, BVD, and FAI and the LST strengthened, "inflection point" of inverse U-shaped curve significantly declined, and their explanation rate of the LST first increased and then decreased, with a maximum value at the 700 m scale. Additionally, the FAI exerted a stronger negative effect, while the BCR and BVD generally had stronger positive effects on the LST as the grid size increased. This study extends our scientific understanding of the complex effect of urban morphology on the LST and is of great practical significance for multiscale urban thermal environment regulation.
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http://dx.doi.org/10.1007/s11356-021-15177-7DOI Listing
July 2021

Atomically Conformal Metal Laminations on Plasmonic Nanocrystals for Efficient Catalysis.

J Am Chem Soc 2021 Jul 2;143(28):10582-10589. Epub 2021 Jul 2.

Creative Research Initiative Center for Nanospace-Confined Chemical Reactions (NCCR) and Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea.

Despite the enormous application potential, methods for conformal few-atomic-layer deposition on colloidal nanocrystals (NCs) are scarce. Similar to the process of lamination, we introduce a "confine and shine" strategy to homogeneously modify the different surface curvatures of plasmonic NCs with ultrathin conformal layers of diverse catalytic noble metals. This self-limited epitaxial skinlike metal growth harvests the localized surface plasmon resonance to induce reduction chemistry directly on the NC surface, confined inside hollow silica. This strategy avoids any kinetic anisotropic metal deposition. Unlike the conventional thick, anisotropic, and dendritic shells, which show severe nonradiative damping, the skinlike metal lamination preserves the key plasmonic properties of the core NCs. Consequently, the plasmonic-catalytic hybrid nanoreactors can carry out a variety of organic reactions with impressive rates.
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http://dx.doi.org/10.1021/jacs.1c05753DOI Listing
July 2021

Circulating tumor DNA reveals genetic traits of patients with intraductal carcinoma of the prostate.

BJU Int 2021 Jun 29. Epub 2021 Jun 29.

Department of Urology, Institute of Urology, West China Hospital, Sichuan University, 610041, Chengdu, China.

Objectives: To investigate the genetic alterations of prostate cancer (PCa) patients with and without intraductal carcinoma of the prostate (IDC-P).

Materials And Methods: We performed targeted sequencing of plasma cell-free DNA on 161 patients of prostate adenocarcinoma (PAC) with IDC-P and 84 cases without IDC-P. Genomic alterations were compared between these two groups. The association between genetic alterations and patients' survival outcomes was also explored.

Results: Totally, we identified 29.8% (48/161) and 21.4% (18/84) patients with and without IDC-P harbored genomic alterations in DNA repair pathway, respectively (P=0.210). Pathogenic germline DNA repair alterations were frequently detected in IDC-P carriers compared to IDC-P non-carries (11.8% [19/161] vs. 2.4% [2/84], P=0.024). Germline BRCA2 and somatic CDK12 defects were specifically identified in IDC-P carriers relative to PAC (BRCA2: 8.7% [14/161] vs. 0% and CDK12: 6.8% [11/161] vs. 1.2% [1/84]). Patients with IDC-P had a distinct AR pathway alteration, characterized by an enrichment of NCOR2 mutations against patients with pure PAC (21.1% [34/161] vs. 6.0% [5/84], P=0.004). Increased AR alterations were detected in patients harbouring tumors with IDC-P proportion≥10% versus cohort with IDC-P proportion<10% (6.4% [5/78] vs. 18.1% [15/83], P=0.045). For IDC-P carries, TP53 mutation was associated with shorter castration-resistant free survival (median 10.9-Mo vs. 28.9-Mo, P=0.026), and BRCA2 alteration was related to rapid PSA progression for those receiving abiraterone treatment (median 9.1-Mo vs 11.9-Mo, P=0.036).

Conclusion: Our findings provide genomic evidence explaining the aggressive phenotype of tumors with IDC-P, highlighting the potential therapeutic strategies for this patient population.
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http://dx.doi.org/10.1111/bju.15530DOI Listing
June 2021

Method to obtain the initial value for the inverse design in nanophotonics based on a time-reversal technique.

Opt Lett 2021 Jun;46(12):2815-2818

In the inverse design of nanophotonic devices, mathematical optimization methods are generally used to perform local optimization in the design region to obtain the physical structure that meets design expectations. These methods usually produce good structures. However, due to the lack of physical considerations, most of the inverse design methods for nanophotonic devices use random initial topology as the initialization for optimization, which will inevitably cause a waste of computing resources. In this Letter, we propose a method based on a time-reversal technique to quickly determine the induced source of the physical structure in the design region and, thus, obtain the initial topological structure of the nanophotonic devices. For a nanophotonic 90°-bend waveguide and 90°-bend power splitter waveguide, numerical examples show that the initial topology obtained by our method not only has good initial performance, but also can be used as a reasonable initialization for inverse design.
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http://dx.doi.org/10.1364/OL.428068DOI Listing
June 2021

Atrial reconstruction, distal gastrectomy with Ante-situm liver resection and autotransplantation for hepatocellular carcinoma with atrial tumor thrombus: A case report.

Medicine (Baltimore) 2021 May;100(19):e25780

Department of Liver Transplantation & Laparoscopic Surgery, Center of Organ Transplantation, The 1st Affiliated Hospital of Xinjiang Medical University.

Rationale: Hepatocellular with tumor thrombi extending into 3 hepatic veins (HVs) and right atrium presents as a real clinical challenge. We report the first documented case of surgical resection of an advanced hepatocellular carcinoma (HCC) with extensive invasion to distal stomach, atrium and hepatic vasculatures.

Patient Concerns: We present a case of 48-years old man with abdominal mass accompanying shortness of breath after activities.

Diagnoses: Preoperative examination revealed giant HCC with tumor thrombi extending into portal vein, HVs, inferior vena cava, and atrium.

Interventions: Distal stomach involvement was confirmed at surgery and, distal gastrectomy, atrial reconstruction and ante-situm liver resection and autotransplantation under cardio-pulmonary bypass were performed.

Outcomes: The operation time was 490 minutes, extracorporeal circulation time 124 minutes, and anhepatic time 40 minutes. Postoperative follow-up revealed normal hepatic and cardiac function with no sign of recurrence.

Lessons: This case illustrates that the extensive invasion of HCC to major vasculature and adjacent organs may not necessarily preclude the liver autotransplantation with multi-visceral resection as the treatment option of extremely advanced HCC patients.
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http://dx.doi.org/10.1097/MD.0000000000025780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133267PMC
May 2021

Dual Network Sponge for Compressible Lithium-Ion Batteries.

Small 2021 07 26;17(26):e2100911. Epub 2021 May 26.

School of Materials Science and Engineering, Peking University, Beijing, 100871, China.

Compressible energy devices have received increasing attention with the rapid development of flexible electronics and wearable devices due to their size adaptability and functional stability. However, it is hard to simultaneously achieve satisfactory energy density and mechanical stability for electrodes. Here an open-porous dual network sponge (DNS) with two networks of highly conductive carbon nanotubes and Li -intercalating TiO -B nanowires is synthesized and employed as compressible lithium ion battery electrodes. All 1D components inside the DNS mutually penetrate with each other to form two physically distinct but functionally coupling networks, endowing DNS excellent compressibility and stability. A prototype compressible lithium-ion battery (C-LIB) is also demonstrated, in which the DNS exhibits a specific capacity of >238 mAh g under static 50% strain, and further in situ measurements show that under 1000 times of cyclic strains, DNS can charge and discharge normally maintaining a high capacity of 240 mAh g and exhibits robustness to fast strain rates up to 500% min . The dual network structure can be extended to design high-performance compliant electrodes that are promising to serve in future compressible and deformable electronics and energy systems.
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http://dx.doi.org/10.1002/smll.202100911DOI Listing
July 2021

Electronic and Structural Transitions of LaAlO /SrTiO Heterostructure Driven by Polar Field-Assisted Oxygen Vacancy Formation at the Surface.

Adv Sci (Weinh) 2021 Jul 24;8(14):e2002073. Epub 2021 May 24.

Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.

The origin of 2D electron gas (2DEG) at LaAlO /SrTiO (LAO/STO) interfaces has remained highly controversial since its discovery. Various models are proposed, which include electronic reconstruction via surface-to-interface charge transfer and defect-mediated doping involving cation intermixing or oxygen vacancy (V ) formation. It is shown that the polar field-assisted V formation at the LAO/STO surface plays critical roles in the 2DEG formation and concurrent structural transition. Comprehensive scanning transmission electron microscopy analyses, in conjunction with density functional theory calculations, demonstrate that V forming at the LAO/STO surface above the critical thickness (t ) cancels the polar field by doping the interface with 2DEG. The antiferrodistortive (AFD) octahedral rotations in LAO, which are suppressed below the t , evolve with the formation of V above the t . The present study reveals that local symmetry breaking and shallow donor behavior of V induce the AFD rotations and relieve the electrical field by electron doping the oxide heterointerface.
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http://dx.doi.org/10.1002/advs.202002073DOI Listing
July 2021

Dynamic multidisciplinary team discussions can improve the prognosis of metastatic castration-resistant prostate cancer patients.

Prostate 2021 Aug 24;81(11):721-727. Epub 2021 May 24.

Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Background: Multidisciplinary team (MDT) management is a popular treatment paradigm in managing cancer patients, which provides fully-discussed, interdisciplinary treatment recommendations for patients. However, there has been a lack of data on its actual impact on the overall survival (OS) of metastatic castration-resistant prostate cancer (mCRPC) patients. mCRPC is the end stage of prostate cancer, facing a treatment dilemma of overwhelming options; therefore, we hypothesize dynamic MDT discussions can be helpful in comprehensively managing these patients.

Methods: We retrospectively collected 422 mCRPC patients' clinical information from 2013 to 2020 from our institute. Patients can voluntarily choose whether to enroll in the dynamic MDT group, which includes discussions at CRPC diagnosis and subsequent disease progression. All patients were followed up regularly, and OS from CRPC diagnosis to death was set as the endpoint of this study.

Results: Participating in MDT discussions is a favorable independent indicator of longer overall survival (median OS: MDT (+): 39.7 months; MDT (-): 27.0 months, hazard ratio: 0.549, p = .001). Moreover, this survival benefit of MDT remained in subgroups with first-line therapy [median OS: MDT (+): not reached; MDT (-): 27.0 months, p = .001) and with multi-line therapy until the end of follow-up (median OS: MDT (+): 36.7 months; MDT (-): 25.6 months, p = .044).

Conclusion: Therefore, regular MDT discussions are valuable in the management of mCRPC patients. Clinicians are encouraged to tailor MDT discussions dynamically to provide mCRPC patients with a better and more individualized treatment plan and more prolonged survival. Take-home messages ● The MDT model is defined as dynamic MDT discussions at the time of mCRPC diagnosis and each time they progressed later on throughout the disease management. ● Prostate cancer MDT usually includes specialists in urologic oncology, pathology, chemotherapy, radiotherapy, ultrasound, imaging and nuclear medicine. ● MDT model can benefit mCRPC patients in terms of overall survival.
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http://dx.doi.org/10.1002/pros.24167DOI Listing
August 2021

Comparative Investigation into the Complexation and Extraction Properties of Tridentate and Tetradentate Phosphine Oxide-Functionalized 1,10-Phenanthroline Ligands toward Lanthanides and Actinides.

Chemistry 2021 Jul 8;27(41):10717-10730. Epub 2021 Jun 8.

College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, P. R. China.

Two new phosphine oxide-functionalized 1,10-phenanthroline ligands, tetradentate 2,9-bis(butylphenylphosphine oxide)-1,10-phenanthroline (BuPh-BPPhen, L ) and tridentate 2-(butylphenylphosphine oxide)-1,10-phenanthroline (BuPh-MPPhen, L ), were synthesized and studied comparatively for their coordination with trivalent actinides and lanthanides. The complexation mechanisms of these two ligands toward trivalent f-block elements were thoroughly elucidated by NMR spectroscopy, UV/vis spectrophotometry, fluorescence spectrometry, single-crystal X-ray diffraction, solvent extraction, and theoretical calculation methods. NMR titration results demonstrated that 1 : 1 and 1 : 2 (metal to ligand) lanthanides complexes formed for L , whereas 1 : 1, 1 : 2 and 1 : 3 lanthanide complexes formed for L in methanol. The formation of these species was validated by fluorescence spectrometry, and the corresponding stability constants for the complexes of Nd with L and L were determined by using UV/vis spectrophotometry. Structures of the 10-coordinated 1 : 1-type complexes of EuL (NO ) and [EuL (NO ) (H O)] Et O in the solid state were characterized by X-ray crystallography. In solvent-extraction experiments, L exhibited extremely strong extraction ability for both Am and Eu , whereas L showed nearly no extraction toward Am or Eu due to its high hydrophilicity. Finally, the structures and bonding natures of the complex species formed between Am /Eu and L /L were analyzed in DFT calculations.
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http://dx.doi.org/10.1002/chem.202101224DOI Listing
July 2021

, a Key Regulator of a Transitive Triplet, Acts on the TGF-β Signaling Pathway and Contributes to High-Altitude Adaptation of Tibetan Pigs.

Front Genet 2021 15;12:628192. Epub 2021 Apr 15.

College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.

Tibetan pigs are native mammalian species on the Tibetan Plateau that have evolved distinct physiological traits that allow them to tolerate high-altitude hypoxic environments. However, the genetic mechanism underlying this adaptation remains elusive. Here, based on multitissue transcriptional data from high-altitude Tibetan pigs and low-altitude Rongchang pigs, we performed a weighted correlation network analysis (WGCNA) and identified key modules related to these tissues. Complex network analysis and bioinformatics analysis were integrated to identify key genes and three-node network motifs. We found that among the six tissues (muscle, liver, heart, spleen, kidneys, and lungs), lung tissue may be the key organs for Tibetan pigs to adapt to hypoxic environment. In the lung tissue of Tibetan pigs, we identified , , , , , , , , and genes as potential regulators of hypoxia adaption. We found that and genes might simultaneously regulate the gene, forming a complex. This complex, dominated by , may enhance the hypoxia tolerance of Tibetan pigs by mediating the TGF-β signaling pathway. The complex may also affect the PI3K-Akt signaling pathway, which plays an important role in angiogenesis caused by hypoxia. Therefore, we postulate that the complex may be beneficial for Tibetan pigs to survive better in the hypoxia environments. Although further molecular experiments and independent large-scale studies are needed to verify our findings, these findings may provide new details of the regulatory architecture of hypoxia-adaptive genes and are valuable for understanding the genetic mechanism of hypoxic adaptation in mammals.
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http://dx.doi.org/10.3389/fgene.2021.628192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082500PMC
April 2021

Online Adaptive Prediction of Human Motion Intention Based on sEMG.

Sensors (Basel) 2021 Apr 20;21(8). Epub 2021 Apr 20.

School of Computer Science and Technology, Harbin Institute of Technology, Harbin 150000, China.

Accurate and reliable motion intention perception and prediction are keys to the exoskeleton control system. In this paper, a motion intention prediction algorithm based on sEMG signal is proposed to predict joint angle and heel strike time in advance. To ensure the accuracy and reliability of the prediction algorithm, the proposed method designs the sEMG feature extraction network and the online adaptation network. The feature extraction utilizes the convolution autoencoder network combined with muscle synergy characteristics to get the high-compression sEMG feature to aid motion prediction. The adaptation network ensures the proposed prediction method can still maintain a certain prediction accuracy even the sEMG signals distribution changes by adjusting some parameters of the feature extraction network and the prediction network online. Ten subjects were recruited to collect surface EMG data from nine muscles on the treadmill. The proposed prediction algorithm can predict the knee angle 101.25 ms in advance with 2.36 degrees accuracy. The proposed prediction algorithm also can predict the occurrence time of initial contact 236±9 ms in advance. Meanwhile, the proposed feature extraction method can achieve 90.71±3.42% accuracy of sEMG reconstruction and can guarantee 73.70±5.01% accuracy even when the distribution of sEMG is changed without any adjustment. The online adaptation network enhances the accuracy of sEMG reconstruction of CAE to 87.65±3.83% and decreases the angle prediction error from 4.03∘ to 2.36∘. The proposed method achieves effective motion prediction in advance and alleviates the influence caused by the non-stationary of sEMG.
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http://dx.doi.org/10.3390/s21082882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074390PMC
April 2021

Strain-Engineered Nano-Ferroelectrics for High-Efficiency Piezocatalytic Overall Water Splitting.

Angew Chem Int Ed Engl 2021 Jul 9;60(29):16019-16026. Epub 2021 Jun 9.

School of Materials Science and Engineering, University of New South Wales, Sydney, New South, Wales, 2052, Australia.

Developing nano-ferroelectric materials with excellent piezoelectric performance for piezocatalysts used in water splitting is highly desired but also challenging, especially with respect to reaching large piezo-potentials that fully align with required redox levels. Herein, heteroepitaxial strain in BaTiO nanoparticles with a designed porous structure is successfully induced by engineering their surface reconstruction to dramatically enhance their piezoelectricity. The strain coherence can be maintained throughout the nanoparticle bulk, resulting in a significant increase of the BaTiO tetragonality and thus its piezoelectricity. Benefiting from high piezoelectricity, the as-synthesized blue-colored BaTiO nanoparticles possess a superb overall water-splitting activity, with H production rates of 159 μmol g  h , which is almost 130 times higher than that of the pristine BaTiO nanoparticles. Thus, this work provides a generic approach for designing highly efficient piezoelectric nanomaterials by strain engineering that can be further extended to various other perovskite oxides, including SrTiO , thereby enhancing their potential for piezoelectric catalysis.
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http://dx.doi.org/10.1002/anie.202103112DOI Listing
July 2021

Correction to: Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer.

Cancer Cell Int 2021 Mar 25;21(1):183. Epub 2021 Mar 25.

Department of Pharmacy, Xin Hua Hospital, Shanghai Jiao Tong University, School of Medicine, 1665 Kongjiang Road, Shanghai, 200092, China.

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http://dx.doi.org/10.1186/s12935-021-01876-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995568PMC
March 2021

Functionalization of freeform curved surfaces by shaped femtosecond laser pulses in the propagation axis.

Opt Express 2021 Feb;29(4):5487-5496

With ultrashort pulse durations and ultrahigh peak intensities, ultrafast lasers can create different types of micro/nano-structures to functionalize the processed surface with new properties. However, the applications of this method on freeform surfaces are still limited by the short length of a laser focusing spot and complex control of the 3D moving trajectory in the fabrication process. In this paper, we overcome this problem by shaping the on-axis intensity along the propagation axis using the spatial light modulator. By designing the phase mask, we increased the length of the stable-intensity zone (intensity fluctuation < 10%) by more than 3 times compared to that of an unshaped Bessel beam. The energy deposition was also optimized to be less than 2% fluctuation based on simulations. Using this method, we fabricated micro/nano structures on 3D surfaces at different fluences and demonstrated various properties including colorization, anti-reflection, and hydrophobicity in large height range. We demonstrated the applications of the proposed method in creating hydrophobicity on complex freeform syringe tip surfaces. This improved the minimum manipulatable volume of a liquid droplet to 2 times smaller compared with untreated syringe, thus greatly extending its performance for micro-droplet manipulation. This method offers an alternative approach for reliable and affordable freeform curved-surface processing.
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http://dx.doi.org/10.1364/OE.418663DOI Listing
February 2021

A Direct and Sensitive Method for Determination of 5-Fluorouracil in Colorectal Cancer Cells: Evaluating the Effect of Stromal Cell on Drug Resistance of Cancer Cells.

J Anal Methods Chem 2021 25;2021:6689488. Epub 2021 Feb 25.

Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.

Fibroblasts in the stroma play a critical role in tumor evolution. In this study, we assessed the influence of colonic fibroblasts on colon cancer cells treated with 5-fluorouracil (5-FU), and mouse colon cancer cell lines MC38 and colonic fibroblasts NIH3T3 were used in this study. A sensitive and rapid UHPLC-MS/MS method for the quantitation of 5-FU from the cell and their medium has been successfully developed and validated. The cells were lysed with methanol, and the mixture was evaporated and then redissolved to extract intracellular 5-FU. The analysis was performed on UHPLC-MS/MS using an Atlantis T3-C18 column (3 m, 2. 1 ∗ 100 mm) and gradient elution with acetonitrile and 0.1% formic acid in water. Method validation included the following parameters: the matrix effect range 88.82%-93.64% and the recovery range 93.52%-94.56%. The intraday and interday precision and accuracy were <11% and within ±6%, and the stability, specificity, carry-over, dilution effect, and linearity all conformed to the criteria. The method was applied to detect the concentration of 5-FU inside cells and cell culture medium. The preliminary results present that NIH3T3 could enhance the drug resistance of MC38 to 5-FU with a decreased intracellular concentration of 5-FU in MC38, which showed a positive relationship with NIH3T3 number.
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http://dx.doi.org/10.1155/2021/6689488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932793PMC
February 2021

Curcumin suppresses the malignancy of non-small cell lung cancer by modulating the circ-PRKCA/miR-384/ITGB1 pathway.

Biomed Pharmacother 2021 Jun 6;138:111439. Epub 2021 Mar 6.

Department of Clinical Oncology, The Affiliated Hospital of Shandong University of TCM, Jinan, Shandong, China. Electronic address:

Background: Curcumin exerts a suppressive effect in tumor growth by acting as a modulator of multiple molecular targets. Circular RNA hsa_circ_0007580 (circ-PRKCA) accelerates the tumorigenesis of non-small cell lung cancer (NSCLC). However, whether curcumin can regulate circ-PRKCA to inhibit NSCLC progression is unclear.

Methods: Cell viability, colony formation, apoptosis, migration, and invasion were analyzed using Cell Counting Kit-8 (CCK-8), plate clone, flow cytometry, or transwell assay. Expression of circ-PRKCA, microRNA (miR)-384, and ITGB1 mRNA (integrin subunit beta 1) mRNA were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Curcumin repressed NSCLC growth through regulating circ-PRKCA expression was validated by xenograft assay. The targeting relationship between circ-PRKCA or ITGB1 and miR-384 was verified by dual-luciferase reporter assay. The level of ITGB1 protein was measured by western blotting.

Results: Circ-PRKCA and ITGB1 expression were elevated in NSCLC tissues and cells, but miR-384 had an opposing tendency. After curcumin treatment, the expression tendency of circ-PRKCA, miR-384, and ITGB1 in NSCLC cells was overturned. Furthermore, curcumin impeded viability, colony formation, migration, invasion, and accelerated apoptosis of NSCLC cells, but these impacts were partially reversed by circ-PRKCA elevation, miR-384 downregulation, or ITGB1 overexpression. Also, the inhibitory effect of curcumin on xenograft tumor was further enhanced after circ-PRKCA knockdown. Notably, circ-PRKCA regulated ITGB1 expression through sponging miR-384 in curcumin-treated NSCLC cells.

Conclusions: Curcumin inhibited NSCLC growth through downregulating circ-PRKCA, which regulated ITGB1 expression by adsorbing miR-384. This study provided a new mechanism to understand how curcumin inhibited the progression of NSCLC.
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http://dx.doi.org/10.1016/j.biopha.2021.111439DOI Listing
June 2021

Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer.

Cancer Cell Int 2021 Feb 25;21(1):136. Epub 2021 Feb 25.

Department of Pharmacy, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai, 200092, China.

Aim: Pemetrexed, a new generation antifolate drug, has been approved for the treatment of locally advanced or metastatic breast cancer. However, factors affecting its efficacy and resistance have not been fully elucidated yet. ATP-binding cassette (ABC) transporters are predictors of prognosis as well as of adverse effects of several xenobiotics. This study was designed to explore whether ABC transporters affect pemetrexed resistance and can contribute to the optimization of breast cancer treatment regimen.

Methods: First, we measured the expression levels of ABC transporter family members in cell lines. Subsequently, we assessed the potential role of ABC transporters in conferring resistance to pemetrexed in primary breast cancer cells isolated from 34 breast cancer patients and the role of ABCC5 in mediating pemetrexed transport and apoptotic pathways in MCF-7 cells. Finally, the influence of ABCC5 expression on the therapeutic effect of pemetrexed was evaluated in an in vivo xenograft mouse model of breast cancer.

Results: The expression levels of ABCC2, ABCC4, ABCC5, and ABCG2 significantly increased in the pan-resistant cell line, and the ABCC5 level in the MCF-7-ADR cell line was 5.21 times higher than that in the control group. ABCC5 expression was inversely correlated with pemetrexed sensitivity (IC, r = 0.741; p < 0.001) in breast cancer cells derived from 34 patients. Furthermore, we found that the expression level of ABCC5 influenced the efflux and cytotoxicity of pemetrexed in MCF-7 cells, with IC values of 0.06 and 0.20 μg/mL in ABCC5 knockout and over-expression cells, respectively. In the in vivo study, we observed that ABCC5 affected the sensitivity of pemetrexed in breast tumor-bearing mice, and the tumor volume was much larger in the ABCC5-overexpressing group than in the control group when compared with their own initial volumes (2.7-fold vs. 1.3-fold).

Conclusions: Our results indicated that ABCC5 expression was associated with pemetrexed resistance in vitro and in vivo, and it may serve as a target or biomarker for the optimization of pemetrexed regimen in breast cancer treatment.
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http://dx.doi.org/10.1186/s12935-021-01842-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908708PMC
February 2021

Nontrivial Replication of Loci Detected by Multi-Trait Methods.

Front Genet 2021 3;12:627989. Epub 2021 Feb 3.

Biostatistics Group, School of Life Sciences and School of Ecology, Sun Yat-sen University, Guangzhou, China.

The ever-growing genome-wide association studies (GWAS) have revealed widespread pleiotropy. To exploit this, various methods that jointly consider associations of a genetic variant with multiple traits have been developed. Most efforts have been made concerning improving GWAS discovery power. However, how to replicate these discovered pleiotropic loci has yet to be discussed thoroughly. Unlike a single-trait scenario, multi-trait replication is not trivial considering the underlying genotype-multi-phenotype map of the associations. Here, we evaluate four methods for replicating multi-trait associations, corresponding to four levels of replication strength. Weak replication cannot justify pleiotropic genetic effects, whereas strong replication using our developed correlation methods can inform consistent pleiotropic genetic effects across the discovery and replication samples. We provide a protocol for replicating multi-trait genetic associations in practice. The described methods are implemented in the free and open-source R package MultiABEL.
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http://dx.doi.org/10.3389/fgene.2021.627989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886991PMC
February 2021

Unfolding the Extraction and Complexation Behaviors of Trivalent f-Block Elements by a Tetradentate N,O-Hybrid Phenanthroline Derived Phosphine Oxide Ligand.

Inorg Chem 2021 Feb 27;60(4):2805-2815. Epub 2021 Jan 27.

College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.

In this work, a tetradentate N,O-hybrid 2,9-bis(diphenylphosphine oxide)-1,10-phenanthroline (Ph-BPPhen) ligand was studied for the coextraction of trivalent f-block elements from nitric acid media. The extraction as well as the complexation behaviors of Ph-BPPhen with f-block elements were thoroughly investigated using P and H NMR spectrometry, UV-vis spectrophotometry, single crystal X-ray diffraction, and density functional theoretical (DFT) calculation. Ph-BPPhen exhibits remarkably extraction ability for both Am(III) and Eu(III) and more than 99.5% of Am(III) and Eu(III) were extracted from 1.0 M HNO solution. Slope analysis suggests that both 2:1 and 1:1 ligand/metal complexes were probably formed during the extraction. The 1:1 and 2:1 Ln(III) complexes with Ph-BPPhen were also identified in CHOH solution by NMR spectrometry, and the stability constants were determined via UV-vis spectrophotometry. Structures of the 1:1 Eu(Ph-BPPhen)(NO) and Am(Ph-BPPhen)(NO) complexes were further elucidated by single X-ray crystallography and DFT calculations. The higher extractability of Ph-BPPhen toward trivalent Am(III) and Eu(III) compared with the previously reported phenanthroline-derived amide and phosphonate ligands was attributed to the stronger affinity of the -P═O(R) group to metal ions. The results from this work indicate that the N,O-hybrid 1,10-phenanthroline derived phosphine oxide ligand can serve as a new and promising candidate for coextraction of trivalent f-block elements in the treatment of nuclear waste.
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http://dx.doi.org/10.1021/acs.inorgchem.0c03727DOI Listing
February 2021

Probing the structure-antioxidant activity relationships of four cinnamic acids porous starch esters.

Carbohydr Polym 2021 Mar 24;256:117428. Epub 2020 Nov 24.

State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, Gansu, China.

For investigation of antioxidant capacity relationship, four cinnamic acids (CNAs), including cinnamic (CA), ferulic (FA), p-coumaric (p-CA) and sinapic (SA) acids, were selected to modify porous starch (PS) with different degrees of substitution by esterification, respectively. The ester linkage of CNAs modified PS was confirmed by H NMR, C solid-state NMR and FT-IR. The porous structure was maintained after esterification. Three in vitro antioxidant assays were applied to measure antioxidant capacities. The order of antioxidant capacity was [email protected] > [email protected] > [email protected] > [email protected], due to the presence of phenolic hydroxyl groups with hydrogen donating abilities. Besides electron-donating group on ortho or para positions the benzene ring further enhances the hydrogen donating ability and the stability of hydroxyl radical. This study not only investigated the antioxidant mechanism of CNA modified starch derivatives but probed the way for synthesis of biodegradable antioxidant materials for the food industries.
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http://dx.doi.org/10.1016/j.carbpol.2020.117428DOI Listing
March 2021

Nitrogen-rich covalent triazine frameworks for high-efficient removal of anion dyes and the synergistic adsorption of cationic dyes.

Chemosphere 2021 Jun 11;272:129622. Epub 2021 Jan 11.

Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China. Electronic address:

Efficient adsorption of organic dyes from effluent has great importance for ecological and environmental protection. Herein, covalent triazine frameworks (CTFs) were constructed via the polycondensation of melamine and cyanuric chloride directly. Due to the numerous basic nitrogen atoms as high as 58.98 wt%, high BET surface area (670.2 m g), and hierarchical pore structure, CTFs demonstrated selective adsorption of anionic dyes in high capacity (e.g., a maximum adsorption capacity of 1581 mg g for Congo red at 30 °C). The mechanism of the outstanding adsorption performance was carefully verified and ascribed to the electrostatic attraction and hydrogen bonding between CTFs and anionic dyes. The amine groups linking two adjacent triazine rings have primary responsibility for the superior performance. Unexpectedly, CTFs expressed a tuning synergetic effect for removing cationic dyes in aqueous solution coexisting with anionic dyes, exhibiting a great superiority in the specific and comprehensive treatment of organic dyes contaminated water. Furthermore, CTFs were stable and had long-periodic availability for more than 6 times, ensuring the adsorption rate higher than 90%. For better operation, hybrid monolithic aerogels were constructed by incorporating CTFs into polyvinylidene fluoride then casting in melamine resin foams. The obtained aerogels expressed high-efficient removal of anionic dyes coupled with convenient operation. This well-established metal-free porous material is a promising adsorbent candidate for anionic dyes selectively and even synergetic adsorption of cationic dyes in water remediation.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129622DOI Listing
June 2021

Net anthropogenic nitrogen and phosphorus inputs in Pearl River Delta region (2008-2016).

J Environ Manage 2021 Mar 15;282:111952. Epub 2021 Jan 15.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, PR China; University of Chinese Academy of Science, Beijing, 100049, PR China; College of the Environment, China University of Geosciences, Wuhan, 430074, PR China.

Excess inputs of nitrogen (N) and phosphorus (P) are the main contributors of aquatic environmental deterioration. Due to the agricultural and industrial activities in the rapidly urbanized basin, the anthropogenic N and P cycle are significantly different from other regions. In this study, we took the Pearl River Delta as an example and introduced the budget list of N and P in the five survey years, including the net anthropogenic N inputs (NANI) and net anthropogenic P inputs (NAPI). The results revealed that the intensities of NANI and NAPI in this area increased from 2008 to 2010 and then decreased after 2010. The peak values were 21001 kg N kmyr and 4515 kg P kmyr for the intensities of NNAI and NAPI, respectively, while the lowest values decreased to 19186 kg N kmyr and 4103 kg P kmyr in 2016. The most important contribution of NANI and NAPI sources in this area were net N and P inputs for human food and animal feed with an average contribution of 61.41% and 76.83%, which indicated that large amounts of N and P were introduced into the environment through the food system. This study expanded the knowledge on regional environmental management from human dietary consumption, human life consumption, animal consumption and fertilizer consumption. Its reuse will be put into practice by understanding the driving factors of N and P inputs in each region of the basin, combining the urbanization characteristics.
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http://dx.doi.org/10.1016/j.jenvman.2021.111952DOI Listing
March 2021

Robust Multiple Regression.

Entropy (Basel) 2021 Jan 9;23(1). Epub 2021 Jan 9.

Department of Statistics, Rice University, MS-138, 6100 Main Street, Houston, TX 77005, USA.

As modern data analysis pushes the boundaries of classical statistics, it is timely to reexamine alternate approaches to dealing with outliers in multiple regression. As sample sizes and the number of predictors increase, interactive methodology becomes less effective. Likewise, with limited understanding of the underlying contamination process, diagnostics are likely to fail as well. In this article, we advocate for a non-likelihood procedure that attempts to quantify the fraction of bad data as a part of the estimation step. These ideas also allow for the selection of important predictors under some assumptions. As there are many robust algorithms available, running several and looking for interesting differences is a sensible strategy for understanding the nature of the outliers.
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http://dx.doi.org/10.3390/e23010088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826993PMC
January 2021

Integrated Molecular Characterization of Fumarate Hydratase-deficient Renal Cell Carcinoma.

Clin Cancer Res 2021 Mar 7;27(6):1734-1743. Epub 2021 Jan 7.

Department of Radiology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China.

Purpose: Fumarate hydratase-deficient renal cell carcinoma (FH-deficient RCC) is a rare but lethal subtype of RCC. Little is known about the genomic profile of FH-deficient RCC, and the therapeutic options for advanced disease are limited. To this end, we performed a comprehensive genomics study to characterize the genomic and epigenomic features of FH-deficient RCC.

Experimental Design: Integrated genomic, epigenomic, and molecular analyses were performed on 25 untreated primary FH-deficient RCCs. Complete clinicopathologic and follow-up data of these patients were recorded.

Results: We identified that FH-deficient RCC manifested low somatic mutation burden (median 0.58 mutations per megabase), but with frequent somatic copy-number alterations. The majority of FH-deficient RCCs were characterized by a CpG sites island methylator phenotype, displaying concerted hypermethylation at numerous CpG sites in genes of transcription factors, tumor suppressors, and tumor hallmark pathways. However, a few cases (20%) with low metastatic potential showed relatively low DNA methylation levels, indicating the heterogeneity of methylation pattern in FH-deficient RCC. Moreover, FH-deficient RCC is potentially highly immunogenic, characterized by increased tumor T-cell infiltration but high expression of immune checkpoint molecules in tumors. Clinical data further demonstrated that patients receiving immune checkpoint blockade-based treatment achieved improved progression-free survival over those treated with antiangiogenic monotherapy (median, 13.3 vs. 5.1 months; = 0.03).

Conclusions: These results reveal the genomic features and provide new insight into potential therapeutic strategies for FH-deficient RCC.
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http://dx.doi.org/10.1158/1078-0432.CCR-20-3788DOI Listing
March 2021

Hyperspectral face recognition based on sparse spectral attention deep neural networks.

Opt Express 2020 Nov;28(24):36286-36303

Inspired by the robust capability and outstanding performance of convolutional neural networks (CNN) in image classification tasks, CNN-based hyperspectral face recognition methods are worthy of further exploration. However, hyperspectral imaging poses new challenges including high data dimensionality and interference between bands on spectral dimension. High data dimensionality can result in high computational costs. Moreover, not all bands are equally informative and discriminative. The usage of a useless spectral band may even introduce noises and weaken the performance. For the sake of solving those problems, we proposed a novel CNN framework, which adopted a channel-wise attention mechanism and Lasso algorithm to select the optimal spectral bands. The framework is termed as the sparse spectral channel-wise attention-based network (SSCANet) where the SSCA-block focuses on the inter-band channel relationship. Different from other methods which usually select the useful bands manually or in a greedy fashion, SSCA-block can adaptively recalibrate spectral bands by selectively emphasizing informative bands and suppressing less useful ones. Especially, a Lasso constraint strategy can zero out the bands during the training of the network, which can boost the training process by making the weights of bands sparser. Finally, we evaluate the performance of the proposed method in comparison of other state-of-the-art hyperspectral face recognition algorithms on three public datasets HK-PolyU, CMU, and UWA. The experimental results demonstrate that SSCANet based method outperforms the state-of-the-art methods for face recognition on the benchmark.
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http://dx.doi.org/10.1364/OE.404793DOI Listing
November 2020

Panoramic transcriptome analysis and functional screening of long noncoding RNAs in mouse spermatogenesis.

Genome Res 2021 01 16;31(1):13-26. Epub 2020 Dec 16.

Department of Biochemistry and Molecular Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.

Long noncoding RNAs (lncRNAs) have emerged as diverse functional regulators involved in mammalian development; however, large-scale functional investigation of lncRNAs in mammalian spermatogenesis in vivo is lacking. Here, we delineated the global lncRNA expression landscape in mouse spermatogenesis and identified 968 germ cell signature lncRNAs. By combining bioinformatics and functional screening, we identified three functional lncRNAs (, , and ) that directly influence spermatogenesis in vivo. Knocking down hampered the development of round spermatids into elongating spermatids and disrupted key spermatogenic gene expression. Mechanistically, lncRNA localized in the nucleus of round spermatids and occupied the genomic regulatory region of important spermatogenic genes including and These findings provide a valuable resource and framework for future functional analysis of lncRNAs in spermatogenesis and their potential roles in other biological processes.
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http://dx.doi.org/10.1101/gr.264333.120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849387PMC
January 2021