Publications by authors named "Zhinan Zhang"

31 Publications

Autoantibody against angiotensin II type I receptor induces pancreatic β-cell apoptosis via enhancing autophagy.

Acta Biochim Biophys Sin (Shanghai) 2021 May;53(6):784-795

Key Laboratory of Cellular Physiology (Shanxi Medical University), Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan 030001, China.

Autoantibody against the angiotensin II type I receptor (AT1-AA) has been found in the serum of patients with diabetes mellitus (DM). However, it remains unclear whether AT1-AA induces β-cell apoptosis and participates in the development of DM. In this study, an AT1-AA-positive rat model was set up by active immunization, and AT1-AA IgG was purified. INS-1 cells were treated with AT1-AA, and cell viability, apoptosis, and autophagy-related proteins were detected by Cell Counting Kit-8 assay, flow cytometry, and western blot analysis, respectively. Results showed that existence of AT1-AA impaired the islet function and increased the apoptosis of pancreatic islet cells in rats, and the autophagy level in rat pancreatic islet tissues tended to increase gradually with the prolongation of immunization time. AT1-AA markedly reduced INS-1 cell viability, promoted cell apoptosis, and decreased insulin secretion in vitro. In addition, the autophagy level was gradually increased along with the prolongation of AT1-AA treatment time. Meanwhile, it was determined that treatment with autophagy inhibitor 3-methyladenine and angiotensin II type 1 receptor (AT1R) blocker telmisartan could improve insulin secretion and apoptosis in vitro and in vivo. In conclusion, it is deduced that upregulation of autophagy contributed to the AT1-AA-induced β-cell apoptosis and islet dysfunction, and AT1R mediated the signal transduction.
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http://dx.doi.org/10.1093/abbs/gmab049DOI Listing
May 2021

Electroacupuncture alleviated the depression-like behavior by regulating FGF2 and astrocytes in the hippocampus of rats with chronic unpredictable mild stress.

Brain Res Bull 2021 Apr 9;169:43-50. Epub 2021 Jan 9.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, China. Electronic address:

Studies have shown that basic fibroblast growth factor (FGF2) is a neurotrophic factor associated with depression. Electroacupuncture (EA) has been shown to be an effective treatment for depression. In the current study, we observed the effects of EA on hippocampal FGF2 and astrocytes, and further investigated the mechanism underlying antidepressant effect of EA. The chronic unpredictable mild stress (CUMS) method were selected to induce depressive-like behaviors of rats. Paroxetine is a commonly used antidepressant and was used as a positive control drug in this experiment. The male adult Sprague Dawley (SD) rats were randomized to four experimental groups (normal control group, CUMS group, EA group and paroxetine group, n = 10/group). EA intervention was administered once daily for 14 days at acupuncture points Baihui (GV20) and Yintang (GV29). Rats in the paroxetine group received daily paroxetine administered intragastrical. Behavioral test, immunohistochemistry (IHC), western blot (WB) and quantitative real-time PCR (qPCR) were conducted to evaluate the intervene effect and the changes of FGF2 and astrocyte marker (glial fibrillary acidic protein, GFAP). The results showed that EA and paroxetine could improve depression-like behavior in CUMS rats, and up-regulated the expression level of FGF2 in the hippocampus, increased GFAP protein expression and the mean optical density of GFAP-immunoreactive astrocyte (GFAP-ir astrocyte). Our findings have identified that EA could ameliorate depressive-like behaviors possibly by regulating the expression of FGF2 in the hippocampus, and the mechanism might be related to the effect of FGF2 on astrocytes.
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http://dx.doi.org/10.1016/j.brainresbull.2021.01.005DOI Listing
April 2021

EA Ameliorated Depressive Behaviors in CUMS Rats and Was Related to Its Suppressing Autophagy in the Hippocampus.

Neural Plast 2020 22;2020:8860968. Epub 2020 Sep 22.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, China.

Autophagy is confirmed to be involved in the onset and development of depression, and some antidepressants took effect by influencing the autophagic process. Electroacupuncture (EA), as a common complementary treatment for depression, may share the mechanism of influencing autophagy in the hippocampus like antidepressants. To investigate that, sixty Sprague-Dawley rats firstly went through chronic unpredictable mild stress (CUMS) model establishment, and 15 rats were assigned to a control group. After modeling, 45 successfully CUMS-induced rats were randomly divided to 3 groups: CUMS, selective serotonin reuptake inhibitor (SSRI), and EA groups (15 rats per group), to accept different interventions for 2 weeks. A sucrose preference test (SPT), weighing, and open field test (OFT) were measurement for depressive behaviors of rats. Transmission electron microscope (TEM), immunohistochemistry (IHC), and western blot analysis were used to evaluate the autophagic changes. After that, depression-like behaviors were successfully induced in CUMS models and reversed by SSRI and EA treatments (both < 0.05), but these two therapies had nonsignificant difference between each other ( > 0.05). Autolysosomes observed through TEM in the CUMS group were more than that in the control group. Their number and size in the SSRI and EA groups also decreased significantly. From IHC, the CUMS group showed enhanced positive expression of both Beclin1 and LC3 in CA1 after modeling ( < 0.05), and the LC3 level declined after EA treatments, which was verified by decreased LC3-II/LC3-I in western blot analysis. We speculated that CUMS-induced depression-like behavior was interacted with an autophagy process in the hippocampus, and EA demonstrated antidepressant effects by partly inhibiting autophagy with a decreased number of autolysosomes and level of LC3 along with LC3-II/LC3-I.
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http://dx.doi.org/10.1155/2020/8860968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527933PMC
September 2020

Acupuncture at LR3 and KI3 shows a control effect on essential hypertension and targeted action on cerebral regions related to blood pressure regulation: a resting state functional magnetic resonance imaging study.

Acupunct Med 2021 02 12;39(1):53-63. Epub 2020 Jun 12.

School of the Tradition Chinese Medicine, Southern Medical University, Guangzhou, China.

Objective: The aim of this study was to investigate the effects of acupuncture at LR3 and KI3 on hypertension at different time points and on related cerebral regions using resting-state functional magnetic resonance imaging (rs-fMRI).

Methods: We randomly divided 29 subjects into two groups: Group A (receiving acupuncture at LR3 + KI3; 15 subjects) and group B (receiving acupuncture at LR3 and a sham location not corresponding to any traditional acupuncture point; 14 subjects). Acupuncture was performed. Blood pressure (BP) changes were recorded and analyzed using SPSS 20.0 statistical software. We used a 3.0T MRI scanner and standard GE 8 channel head coil to collect whole brain fMRI data in both groups. Data analysis and processing was based on the R2009a MATLAB platform. REST 1.8 software was used to analyze the whole brain amplitude of low-frequency fluctuation (ALFF).

Results: After acupuncture, a statistically significant reduction in BP at different time points was observed in group A. In group B, a statistically significant reduction was found only in diastolic blood pressure (DBP) and was not sustained. Acupuncture at LR3 + KI3 specifically affected brain areas involved in BP regulation, as well as those involved in auditory sense, speech, vision, movement and sensation.

Conclusion: Acupuncture at LR3 + KI3 showed positive immediate and long-term effects on BP, particularly systolic blood pressure (SBP). After ALFF analysis, we concluded that LR3 + KI3 activates brain areas related to BP regulation. In addition, after acupuncture at LR3 + KI3, a highly targeted effect was observed in brain areas associated with BP. In addition, extracerebral areas involving vision, motion control, cognition and hearing were activated, which could potentially contribute to the mitigation of hypertensive complications in patients in an advanced stage of the disease.
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http://dx.doi.org/10.1177/0964528420920282DOI Listing
February 2021

Electroacupuncture improves synaptic plasticity by regulating the 5-HT1A receptor in hippocampus of rats with chronic unpredictable mild stress.

J Int Med Res 2020 May;48(5):300060520918419

Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Objectives: To investigate the antidepressant effects of electroacupuncture (EA) on chronic unpredictable mild stress (CUMS) in rats, as well as the effects of EA on hippocampal neurons, synaptic morphology, and 5-hydroxytryptamine (HT) receptor expression.

Methods: Forty adult male Wistar rats were randomly divided into normal control, CUMS, EA, and paroxetine groups. CUMS modeling was performed for 21 days, followed by 14 days of intervention: rats in the EA group underwent stimulation of GV20 and GV29 acupuncture points for 30 minutes daily; rats in the paroxetine group were administered paroxetine daily. Behavioral tests, transmission electron microscopy, western blotting, and real-time quantitative polymerase chain reaction were used to evaluate the effects of the intervention.

Results: EA treatment reversed the behavioral changes observed in rats due to CUMS modeling; it also improved the pathological changes in organelles and synaptic structures of hippocampal neurons, and upregulated the protein and mRNA expression levels of 5-HT1A receptor. There were no significant differences in 5-HT1B receptor protein and mRNA expression levels among the groups.

Conclusions: EA treatment can alleviate depression-like symptoms in CUMS rats. The underlying mechanism may include promoting the expression of 5-HT1A receptor mRNA and protein, thereby improving synaptic plasticity in the hippocampus.
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http://dx.doi.org/10.1177/0300060520918419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221223PMC
May 2020

RhB-Embedded Zirconium-Naphthalene-Based Metal-Organic Framework Composite as a Luminescent Self-Calibrating Platform for the Selective Detection of Inorganic Ions.

Chemistry 2020 Feb 24;26(7):1661-1667. Epub 2020 Jan 24.

Provincial Key Laboratory of Advanced Energy Materials, College of Chemistry, Northeast Normal University, Changchun, 130024, P. R. China.

A series of [email protected] composites were synthesized through in situ encapsulation of luminous rhodamine B (RhB) molecules into a blue-emissive zirconium-naphthalene-based metal-organic framework (Zr-MOF). The fabricated [email protected] composites exhibit tunable dual-emissive characteristics due to the process of resonant energy transfer from Zr-MOF to RhB. Notably, one of the [email protected] composites ([email protected]) exhibited a weak emission at 420 nm and a strong emission at 607 nm, for which the two emissions possess large distinctions in location and intensity and can be referenced with each other in sensing analytes. By using relative fluorescence intensity instead of their absolute fluorescence intensity as the detection signals, [email protected] served as a built-in self-calibrated platform to selectively detect Fe and Cr O ions in water. Compared with the pristine Zr-MOF, the [email protected] composite shows enhanced sensing selectivity to Fe and higher sensibility to Cr O . This study displays the advantages of combining organic dyes with robust Zr-MOFs in tuning fluorescence and sensing performance.
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http://dx.doi.org/10.1002/chem.201904448DOI Listing
February 2020

Double-Vacancy Controlled Friction on Graphene: The Enhancement of Atomic Pinning.

Langmuir 2019 Oct 27;35(40):12898-12907. Epub 2019 Sep 27.

The vacancy-enhanced contact friction of graphene is mainly attributed to the vacancy-enhanced out-of-plane deformation flexibility of the graphene and the climbing of the tip out of the vacancy trap (which actually acts as a step edge). However, this mechanism does not apply for explaining the enhanced friction caused by small-sized vacancies that are unable to accommodate the tip, such as single vacancy and double vacancies, which also commonly exist in the graphene. In the present study, by performing a set of classic molecular dynamics simulations, we demonstrated that the double-vacancy defect in graphene substantially enhanced the contact friction when the tip slides over it and the pinning effect of the reconstructed lattice of the double-vacancy defect with atoms at the bottom of the tip dominated such an influence. The underlying mechanism of such an atomic pinning effect and the influence of the normal load, sliding direction, and the sliding velocity were unveiled by analyzing the obtained friction evolution and the atomic configuration and interaction between the tip and the graphene. We believe that the findings presented in this study complete the state-of-art understanding of the nanoscale friction behaviors of vacancy-defected graphene, which is essential for the implementation of their potential control.
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http://dx.doi.org/10.1021/acs.langmuir.9b01672DOI Listing
October 2019

Effects of reduced seawater pH on nematode community composition and diversity in sandy sediments.

Mar Environ Res 2019 Sep 16;150:104773. Epub 2019 Aug 16.

College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China. Electronic address:

The present study investigated the potential effects of seawater acidification on the taxonomic structure and diversity of nematode communities using a microcosm experiment. Nematode samples for the microcosm experiment were collected from the low tidal zone of two sandy beaches with different sediment compositions (medium sand vs. very fine sand) in Qingdao (China). In the microcosm, nematode communities were exposed to nine experimental treatments comprising two pH levels for 56 days: 8.0 (ambient control) and 7.3. Communities were exposed for 0, 7, 14, 28, or 56 days. Results showed that the most distinguishable differences in nematode community structure and diversity indices were caused by sediment type. Reduced pH changed the taxonomic structure of nematode communities in medium sand sediments. An increase in species with higher tolerance to lowered pH occurred as a response and resulted in increased diversity in medium sand sediments. Nematode communities in finer sediments appeared less sensitive to reduced pH.
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http://dx.doi.org/10.1016/j.marenvres.2019.104773DOI Listing
September 2019

Benthic ecological quality assessment of the Bohai Sea, China using marine biotic indices.

Mar Pollut Bull 2019 May 6;142:457-464. Epub 2019 Apr 6.

College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China. Electronic address:

The coastal ecological environments are heavily affected by anthropogenic activities as well as global climate change. Macrofauna can be used as good indicators for benthic ecological quality status due to its relatively slow movement ability under disturbance and a variety of assessment approaches have been established based on the characteristics of macrofauna. The benthic ecological quality of the Bohai Sea, China, is increasingly threatened by anthropogenic activities. In order to accurately assess the benthic ecological quality status in this region, Shannon-Wiener diversity index, ABC curve, AMBI, M-AMBI and macrofaunal feeding evenness index were used. Results showed that the northern Laizhou Bay and Bohai Bay were under slight disturbance while Liaodong Bay, the central Bohai Sea and Bohai Strait were under moderate disturbance. Overall, the ecological quality indicated by the assessment results of AMBI, Shannon-Wiener diversity index and feeding evenness index were better than that by M-AMBI and the ABC curve.
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http://dx.doi.org/10.1016/j.marpolbul.2019.03.055DOI Listing
May 2019

iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression.

Neural Plast 2019 2;2019:7492306. Epub 2019 May 2.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, China.

Hippocampal atrophy is one of the key changes in the brain implicated in the biology of depression. However, the precise molecular mechanism remains poorly understood due to a lack of biomarkers. In this research, we used behavioral experiments to evaluate anxiety and anhedonia levels in depressed rats using chronic unpredictable mild stress (CUMS) modeling. We also used isobaric tag for relative and absolute quantitation (iTRAQ) to identify the differentially expressed hippocampal proteins between depressed and normal rats. Bioinformatics analyses were also performed for a better understanding. The results showed that CUMS rats had higher anxiety and anhedonia levels than control rats, along with hippocampal lesions. Through iTRAQ and bioinformatics analyses, we found that ribosome proteins were significantly downregulated and Ras proteins exhibited a mixed change in the hippocampus of depressed rats. These findings suggest that the expression of hippocampal ribosome lesions and Ras proteins is significantly different in depressed rats than in control rats, providing new insights into the neurobiology of depression.
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http://dx.doi.org/10.1155/2019/7492306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525853PMC
January 2020

Electroacupuncture Improves Antidepressant Effects in CUMS Rats by Protecting Hippocampal Synapse and Mitochondrion: An Ultrastructural and iTRAQ Proteomic Study.

Evid Based Complement Alternat Med 2019 17;2019:3424698. Epub 2019 Apr 17.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province 510515, China.

Electroacupuncture (EA) is considered a complementary therapy for depression. Trials also found that EA has additive benefits when combined with medication compared with medication alone. It is revealed that EA restores altered hippocampal synaptic plasticity in depressed brain. But precise molecular mechanism is poorly understood. Here, we evaluated the therapeutic effects of EA and EA combined with selective serotonin reuptake inhibitor (SSRI) on depressed (CUMS) rats. Then a new proteomics approach, isobaric tag for relative and absolute quantitation (iTRAQ), was used to explore the differential expressed synaptic protein in hippocampus between CUMS and EA-treated rats to identify the possible target molecular mechanism of its effects. We found that EA had additive benefit against depressive behaviors when combined with SSRI. Ultrastructure study on neuron showed significant change in postsynapse density (PSD) and mitochondrion. Through iTRAQ, it is found that synaptic and mitochondrial proteins were significantly changed after EA, consisting with ultrastructure study results. These findings suggest that EA improves antidepressant performance in depressed rats through protecting synaptic and mitochondrial functions in hippocampus.
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http://dx.doi.org/10.1155/2019/3424698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6500624PMC
April 2019

Different Brain Activation after Acupuncture at Combined Acupoints and Single Acupoint in Hypertension Patients: An Rs-fMRI Study Based on ReHo Analysis.

Evid Based Complement Alternat Med 2019 3;2019:5262896. Epub 2019 Jan 3.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province 510515, China.

Background: Acupuncture is proved to be effective on hypertension by numerous studies and resting-state functional magnetic resonance imaging (Rs-fMRI) is a widely used technique to study its mechanism. Along with lower blood pressure, patients with hypertension receiving acupuncture also presented improvement in function of cognition, emotion, language, sematic sensation, and so on. This study was a primary study to explore the acting path of acupuncture at combined acupoints in stimulated brain areas related to such functions.

Methods: In this research, regional homogeneity (ReHo) was applied to analyze the Rs-fMRI image data of brain activities after acupuncture at LR3, KI3, and LR3+KI3 and to compare the differences of functional brain activities between stimulating combined acupoints and single acupoint under pathological conditions. A total of thirty hypertension patients underwent Rs-fMRI scanning before acupuncture treatment and then were randomly divided into three groups following random number table, the LR3 group (3 males and 7 females), the KI3 group (3 males and 7 females), and the LR3+ KI3 group (4 males and 6 females) for needling, respectively. When the 30-min treatment finished, they received a further Rs-fMRI scanning. The Rs-fMRI data before and after the acupuncture treatment were analyzed through ReHo.

Results: Compared with preacupuncture, respectively, ReHo values increased in Brodmann areas (BAs) 3, 18, and 40 and decreased in BAs 7 and 31 in LR3+ KI3 group. However, ReHo values only decreased in BA7 of KI3 group while the results showed no significant difference of brain regions in LR3 group between pre- and postacupuncture. Compared with LR3 group, LR3+KI3 group exhibited decreased ReHo values in BAs 7, 9, and 31. Meanwhile, compared with KI3 group, LR3+KI3 group exhibited increased ReHo values in the BAs 2, 18, 30, and 40 and decreased ReHo values in BA13.

Conclusion: Combined acupoints of LR3 and KI3 could act on wider brain areas than the sum of single acupoints, whose functions include emotional processing, cognition, somatic sensation, spatial orientation, language production, and vision.
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http://dx.doi.org/10.1155/2019/5262896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335668PMC
January 2019

Effects of Yellow Sea Cold Water Mass on marine nematodes based on biological trait analysis.

Mar Environ Res 2018 Oct 28;141:167-185. Epub 2018 Aug 28.

College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.

The Yellow Sea Cold Water Mass (YSCWM) is a seasonal hydrological phenomenon with significant effects on benthic animals. Based on a range of biological traits, including feeding type, tail shape, adult body length, body shape and life history (c-p value), the biological trait analysis (BTA) of marine nematodes in the southern Yellow Sea was studied in June 2003 (summer) and January 2004 (winter) in order to reveal the effects of YSCWM on benthic animals. In terms of biological traits composition of marine nematode assemblages, results of ANOSIM showed that there were no significant differences among sites inside the YSCMW area. However, for spatial distribution, marine nematode assemblages showed significant differences between sites inside and outside of the YSCWM area in terms of body shape, as there was higher percentage of nematodes with slender body shape while lower percentage with stout and long thin body shape at the sites inside the YSCWM area. Results of BIOENV analysis showed that water depth and sediment silt-clay percentage were the most important factors contributed to the differences of biological traits of marine nematode assemblages in summer, while sediment phaeophorbide content best explained the differences of marine nematode assemblages in winter. The existence of the YSCWM may provide a stable environment for marine nematode assemblages, keeping them in a continuous state during the seasonal changes. The higher percentage of nematodes with slender body may be the response to the YSCWM.
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http://dx.doi.org/10.1016/j.marenvres.2018.08.013DOI Listing
October 2018

Time- & Load-Dependence of Triboelectric Effect.

Sci Rep 2018 02 6;8(1):2470. Epub 2018 Feb 6.

School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.

Time- and load-dependent friction behavior is considered as important for a long time, due to its time-evolution and force-driving characteristics. However, its electronic behavior, mainly considered in triboelectric effect, has almost never been given the full attention and analyses from the above point of view. In this paper, by experimenting with fcc-latticed aluminum and copper friction pairs, the mechanical and electronic behaviors of friction contacts are correlated by time and load analyses, and the behind physical understanding is provided. Most importantly, the difference of "response lag" in force and electricity is discussed, the extreme points of coefficient of friction with the increasing normal loads are observed and explained with the surface properties and dynamical behaviors (i.e. wear), and the micro and macro theories linking tribo-electricity to normal load and wear (i.e. the physical explanation between coupled electrical and mechanical phenomena) are successfully developed and tested.
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http://dx.doi.org/10.1038/s41598-018-20937-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802745PMC
February 2018

Response of free-living marine nematodes to the southern Yellow Sea Cold Water Mass.

Mar Pollut Bull 2016 Apr 7;105(1):58-64. Epub 2016 Mar 7.

College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. Electronic address:

The Yellow Sea Cold Water Mass is a remarkable seasonal hydrographic event in the bottom water of the Yellow Sea. In order to reveal the response of free-living marine nematodes to this event, community structure and biodiversity indices of nematodes were studied in June and November 2013. The dominant species were Dorylaimopsis rabalaisi, Spilophorella sp., Daptonema sp., Sabatieria sp. and Parasphaerolaimus sp. In terms of trophic structure, epigrowth feeders were the most dominant group. Correlation analysis showed that Shannon-Wiener diversity index had significantly negative correlation with sediment silt-clay percentage, organic matter content and water content. Results of BIOENV indicated that sediment phaeophorbide content, water content, bottom water salinity and temperature were the most important factors related to nematode community. In conclusion, community structure and biodiversity indices of nematodes were consistent in the two sampling seasons.
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http://dx.doi.org/10.1016/j.marpolbul.2016.02.067DOI Listing
April 2016

Quantitative distribution and functional groups of intertidal macrofaunal assemblages in Fildes Peninsula, King George Island, South Shetland Islands, Southern Ocean.

Mar Pollut Bull 2015 Oct 29;99(1-2):284-91. Epub 2015 Jul 29.

College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.

To evaluate spatial distribution pattern of intertidal macrofauna, quantitative investigation was performed in January to February, 2013 around Fildes Peninsula, King George Island, South Shetland Islands. A total of 34 species were identified, which were dominated by Mollusca, Annelida and Arthropoda. CLUSTER analysis showed that macrofaunal assemblages at sand-bottom sites belonged to one group, which was dominated by Lumbricillus sp. and Kidderia subquadrata. Macrofaunal assemblages at gravel-bottom sites were divided into three groups while Nacella concinna was the dominant species at most sites. The highest values of biomass and Shannon-Wiener diversity index were found in gravel sediment and the highest value of abundance was in sand sediment of eastern coast. In terms of functional group, detritivorous and planktophagous groups had the highest values of abundance and biomass, respectively. Correlation analysis showed that macrofaunal abundance and biomass had significant positive correlations with contents of sediment chlorophyll a, phaeophorbide and organic matter.
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http://dx.doi.org/10.1016/j.marpolbul.2015.07.047DOI Listing
October 2015

Meiofauna and its sedimentary environment as an integrated indication of anthropogenic disturbance to sandy beach ecosystems.

Mar Pollut Bull 2014 Nov 22;88(1-2):260-7. Epub 2014 Sep 22.

College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.

The accuracy and applicability of the Nematode/Copepod index (N/C) in monitoring the effects of environmental disturbances is controversial. In this study, we used an integrated approach that includes both meiofauna and the sedimentary environment to demonstrate a tourism-induced disturbance gradient among sampled beaches. We also analysed the relationships between meiofauna and environmental factors. The results showed that disturbed beaches were characterised by high values of meiofauna abundance, chlorophyll a content, total organic carbon content and N/C but lower levels of dissolved oxygen. The chlorophyll a and dissolved oxygen contents were found to be the most important factors for explaining the disturbance gradient amongst the beaches. The N/C index had a positive relationship with chlorophyll a and a negative relationship with dissolved oxygen. There was no significant relationship between N/C index and total organic carbon content.
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http://dx.doi.org/10.1016/j.marpolbul.2014.08.033DOI Listing
November 2014

Molecular detection of Candidatus Scalindua pacifica and environmental responses of sediment anammox bacterial community in the Bohai Sea, China.

PLoS One 2013 8;8(4):e61330. Epub 2013 Apr 8.

State Key Laboratory of Heavy Oil Processing, Key Laboratory of Bioengineering and Biotechnology in Universities of Shandong, Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, China.

The Bohai Sea is a large semi-enclosed shallow water basin, which receives extensive river discharges of various terrestrial and anthropogenic materials such as sediments, nutrients and contaminants. How these terrigenous inputs may influence the diversity, community structure, biogeographical distribution, abundance and ecophysiology of the sediment anaerobic ammonium oxidation (anammox) bacteria was unknown. To answer this question, an investigation employing both 16S rRNA and hzo gene biomarkers was carried out. Ca. Scalindua bacteria were predominant in the surface sediments of the Bohai Sea, while non-Scalindua anammox bacteria were also detected in the Yellow River estuary and inner part of Liaodong Bay that received strong riverine and anthropogenic impacts. A novel 16S rRNA gene sequence clade was identified, putatively representing an anammox bacterial new candidate species tentatively named "Ca. Scalindua pacifica". Several groups of environmental factors, usually with distinct physicochemical or biogeochemical natures, including general marine and estuarine physicochemical properties, availability of anammox substrates (inorganic N compounds), alternative reductants and oxidants, environmental variations caused by river discharges and associated contaminants such as heavy metals, were identified to likely play important roles in influencing the ecology and biogeochemical functioning of the sediment anammox bacteria. In addition to inorganic N compounds that might play a key role in shaping the anammox microbiota, organic carbon, organic nitrogen, sulfate, sulfide and metals all showed the potentials to participate in the anammox process, releasing the strict dependence of the anammox bacteria upon the direct availability of inorganic N nutrients that might be limiting in certain areas of the Bohai Sea. The importance of inorganic N nutrients and certain other environmental factors to the sediment anammox microbiota suggests that these bacteria were active for the in situ N transforming process and maintained a versatile life style well adapted to the varying environmental conditions of the studied coastal ocean.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0061330PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3620062PMC
October 2013

Decadal change in sublittoral macrofaunal biodiversity in the Bohai Sea, China.

Mar Pollut Bull 2012 Nov 5;64(11):2364-73. Epub 2012 Sep 5.

College of Marine Life Science, Ocean University of China, 5 Yushan Road, 266003 Qingdao, PR China.

The region of the Bohai Sea is among the most densely populated of any marginal sea worldwide, and the sea is of great commercial importance as one of the major fishing areas in China. In this study, sublittoral macrofaunal community data collected during the 2000s was integrated with historical data from the 1980s to 1990s to detect any change in biodiversity status over the past three decades. Biodiversity was assessed through species diversity, taxonomic relatedness and other measures of community structure. Different geographic locations had different scenarios of decadal biodiversity change. We observed a sustained increase in species diversity in Bohai Bay and a cyclic trajectory in Laizhou Bay. In the central part of the Bohai Sea, there was little change in species diversity, whereas taxonomic distinctness tended to increase. A test for departure from the expected value of taxonomic distinctness (Δ(+), Λ(+)) suggested that approximately one-fifth of the sampling stations were subjected to environmental stress and perturbation.
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http://dx.doi.org/10.1016/j.marpolbul.2012.08.014DOI Listing
November 2012

Diversity, abundance, and spatial distribution of sediment ammonia-oxidizing Betaproteobacteria in response to environmental gradients and coastal eutrophication in Jiaozhou Bay, China.

Appl Environ Microbiol 2010 Jul 28;76(14):4691-702. Epub 2010 May 28.

China University of Petroleum, Qingdao, China.

Ongoing anthropogenic eutrophication of Jiaozhou Bay offers an opportunity to study the influence of human activity on bacterial communities that drive biogeochemical cycling. Nitrification in coastal waters appears to be a sensitive indicator of environmental change, suggesting that function and structure of the microbial nitrifying community may be associated closely with environmental conditions. In the current study, the amoA gene was used to unravel the relationship between sediment aerobic obligate ammonia-oxidizing Betaproteobacteria (Beta-AOB) and their environment in Jiaozhou Bay. Protein sequences deduced from amoA gene sequences grouped within four distinct clusters in the Nitrosomonas lineage, including a putative new cluster. In addition, AmoA sequences belonging to three newly defined clusters in the Nitrosospira lineage were also identified. Multivariate statistical analyses indicated that the studied Beta-AOB community structures correlated with environmental parameters, of which nitrite-N and sediment sand content had significant impact on the composition, structure, and distribution of the Beta-AOB community. Both amoA clone library and quantitative PCR (qPCR) analyses indicated that continental input from the nearby wastewater treatment plants and polluted rivers may have significant impact on the composition and abundance of the sediment Beta-AOB assemblages in Jiaozhou Bay. Our work is the first report of a direct link between a sedimentological parameter and the composition and distribution of the sediment Beta-AOB and indicates the potential for using the Beta-AOB community composition in general and individual isolates or environmental clones in the Nitrosomonas oligotropha lineage in particular as bioindicators and biotracers of pollution or freshwater or wastewater input in coastal environments.
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http://dx.doi.org/10.1128/AEM.02563-09DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901714PMC
July 2010

Diversity and distribution of sediment nirS-encoding bacterial assemblages in response to environmental gradients in the eutrophied Jiaozhou Bay, China.

Microb Ecol 2009 Jul 19;58(1):161-9. Epub 2008 Nov 19.

Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266555, China.

A gene-clone-library-based molecular approach was used to study the nirS-encoding bacteria-environment relationship in the sediments of the eutrophic Jiaozhou Bay. Diverse nirS sequences were recovered and most of them were related to the marine cluster I group, ubiquitous in estuarine, coastal, and marine environments. Some NirS sequences were unique to the Jiaozhou Bay, such as the marine subcluster VIIg sequences. Most of the Jiaozhou Bay NirS sequences had their closest matches originally detected in estuarine and marine sediments, especially from the Chesapeake Bay, indicating similarity of the denitrifying bacterial communities in similar coastal environments in spite of geographical distance. Multivariate statistical analyses indicated that the spatial distribution of the nirS-encoding bacterial assemblages is highly correlated with environmental factors, such as sediment silt content, NH4+ concentration, and OrgC/OrgN. The nirS-encoding bacterial assemblages in the most hypernutrified stations could be easily distinguished from that of the least eutrophic station. For the first time, the sedimentological condition was found to influence the structure and distribution of the sediment denitrifying bacterial community.
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http://dx.doi.org/10.1007/s00248-008-9469-5DOI Listing
July 2009

In situ cationic ring-opening polymerization and quaternization reactions to confine ferricyanide onto carbon nanotubes: a general approach to development of integrative nanostructured electrochemical biosensors.

Anal Chem 2008 Sep 1;80(17):6587-93. Epub 2008 Aug 1.

Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, the Chinese Academy of Sciences (CAS), Beijing 100080, China.

This study demonstrates a new and relatively general route to the development of multiwalled carbon nanotube (MWNT)-based integrative electrochemical biosensors by confining ferricyanide redox mediator onto MWNTs. The ferricyanide-confined MWNTs are synthesized first through grafting of epoxy chloropropane onto MWNTs with in situ cationic ring-opening polymerization and then introducing the positively charged methylimidazolium moieties into the grafted polymer with a quaternization reaction. The grafted polymers with positively charged methylimidazolium moieties tethered onto MWNTs can essentially be used to confine redox-active ferricyanide onto MWNTs to form a redox mediator-confined nanocomposite with a good stability and excellent electrochemical property. The synthetic nanocomposite with surface-confined ferricyanide is demonstrated to be well-competent as the efficient electronic transducers for the general development of electrochemical biosensors upon combination with biorecognition units, which is illustrated by using glucose oxidase and laccase as two model biorecognition units. This study essentially paves a facile and general approach to the development of integrative nanostructured electrochemical biosensors.
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http://dx.doi.org/10.1021/ac800733tDOI Listing
September 2008

Diversity and spatial distribution of sediment ammonia-oxidizing crenarchaeota in response to estuarine and environmental gradients in the Changjiang Estuary and East China Sea.

Microbiology (Reading) 2008 Jul;154(Pt 7):2084-2095

Life Sciences and Technology College, Ocean University of China, Qingdao 266003, China.

Ammonia-oxidizing archaea (AOA) have recently been found to be potentially important in nitrogen cycling in a variety of environments, such as terrestrial soils, wastewater treatment reactors, marine waters and sediments, and especially in estuaries, where high input of anthropogenic nitrogen is often experienced. The sedimentary AOA diversity, community structure and spatial distribution in the Changjiang Estuary and the adjacent East China Sea were studied. Multivariate statistical analysis indicated that the archaeal amoA genotype communities could be clustered according to sampling transects, and the station located in an estuarine mixing zone harboured a distinct AOA community. The distribution of AOA communities correlated significantly with the gradients of surface-water salinity and sediment sorting coefficient. The spatial distribution of putative soil-related AOA in certain sampling stations indicated a strong impact of the Changjiang freshwater discharge on the marine benthic microbial ecosystem. Besides freshwater, nutrients, organic matter and suspended particles, the Changjiang Diluted Water might also contribute to the transport of terrestrial archaea into the seawater and sediments along its flow path.
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http://dx.doi.org/10.1099/mic.0.2007/013581-0DOI Listing
July 2008

[Analysis of cell components of CD59+ normal cells and abnormal clons in bone marrow of patients with paroxysmal nocturnal hemoglobinuria].

Sichuan Da Xue Xue Bao Yi Xue Ban 2003 Apr;34(2):230-3

Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.

Objective: To explore the cell components of normal cells and abnormal clons in bone marrow of patients with paroxysmal nocturnal hemoglobinuria (PNH).

Methods: Bone marrow nuclear cells (BMNCs) were labeled by immunofluorescence and analyzed by flow cytometry.

Results: 1. There was significant difference in BMNCs between normal CD59+ cells and abnormal clons (CD59-) cells, the normal CD59+ BMNCs were mainly composed of the cells in lymphocyte-window, while the abnormal CD59- BMNCs were mainly in blast window and in granulocyte-window. 2. The CD34+ cells in CD59+ BMNCs were also markedly decreased when compared with their CD59- counterparts, and the decrease of CD34+ cells in CD59+ BMNCs was more obvious in lymphocyte window than that in blast window.

Conclusion: The constituents of BMNCs and the amount of CD34+ cells in CD59+ and CD59- cell populations of PNH patients are significantly different, the normal hematopoiesis is exhausted. On account of the defects in normal hematopoietic cells in patients with PNH, the feasibility of using autologous bone marrow transplantation following purging of abnormal clons as a treatment of PNH should be carefully judged, the normal hematopoietic cells alone might not be able to sustain adequate hematopoiesis without further manipulations.
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April 2003

[Apoptosis and differentiation induced by sodium selenite combined with all-trans retinoic acid (ATRA) in NB4 cells].

Zhonghua Xue Ye Xue Za Zhi 2002 Dec;23(12):628-30

Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences and PUMC Hospital, CAMS & PUMC, Beijing 100005, China.

Objective: To study the effects of low dose sodium selenite combined with all-trans retinoic acid (ATRA) on apoptosis and differentiation of human acute promyelocytic leukemia (APL) NB4 cells.

Methods: Apo-ptosis was detected by translocation of phosphatidylserine (PS) with a Annexin-V kit and DNA fragmentation by agarose gel electrophoresis analysis, cell differentiation was studied by flow cytometry of CD(11b) expression and NBT reduction assay.

Results: Five micromol/L sodium selenite or 0.1 micromol/L ATRA alone could not induce apoptosis of NB4 cells within 48 hours. However, combination of the two drugs at the same doses as above could induce significant apoptosis in 48 hours characterized by increased PS translocation and DNA ladder. Sodium selenite at concentration of 2 micromol/L was not able to induce differentiation of NB4 cells, but when combined with 0.1 micromol/L ATRA, CD(11b) expression and NBT reduction were increased as compared with that of 0.1 micromol/L ATRA alone.

Conclusion: Low dose sodium selenite could enhance the effects of low dose ATRA in inducing apoptosis and differentiation of NB4 cells.
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December 2002

[Study of lymphocyte subsets and its activated-molecules in patients with paroxysmal nocturnal hemoglobinuria].

Zhonghua Xue Ye Xue Za Zhi 2002 Nov;23(11):571-3

Department of Respiration, Beijing Tongren Hospital, Capital Medical College, Beijing 100730, China.

Objective: To investigate the relationship between subsets of lymphocytes and between its activated status and the clinical manifestations in patients with PNH, and to unfold immunological mechanism in the pathogenesis of PNH.

Methods: The peripheral blood mononuclear cells (PBMNC) from 18 PNH patients and 20 controls were separated into two subpopulations using anti-CD(59) monoclonal antibody combined with goat-anti-mouse IgG immunomagnetic beads. CD(3)(+), CD(4)(+) and CD(8)(+) lymphocyte subsets were detected by flow cytometry. In 6 newly diagnosed patients, phenotypes associated with T cell activation such as CD(28)(+)/CD(4)(+) or CD(8)(+) cells, CD(8)(+) CD(38)(+) cells, and HLA-DR(+)/CD(4)(+) or CD(8)(+), and NK (CD(3)(-) CD(16)(+)) cells were detected in the peripheral blood.

Result: Patients with PNH showed significantly increased CD(3)(+) CD(8)(+)/CD(3)(+) CD(4)(+) ratio as compared with controls (1.22 +/- 0.51 vs 0.86 +/- 0.27, P < 0.05), and the CD(3)(+) CD(8)(+)/CD(3)(+) CD(4)(+) ratio in CD(59)(-) PBMC was higher than that in CD(59)(+) PBMC (2.31 +/- 1.56 vs 0.62 +/- 0.27, P < 0.05). The ratios of CD(4)(+) CD(28)(+)/CD(4)(+) markedly decreased and CD(8)(+)HLA-DR(+)/CD(8)(+) increased.

Conclusion: Patients with PNH appear to have abnormalities in their lymphocytes. Increased ratios of CD(3)(+) CD(8)(+)/CD(3)(+) CD(4)(+) and HLA-DR(+) CD(8)(+)/CD(8)(+) lymphocytes as well as declined ratio of CD(4)(+) CD(28)(+)/CD(4)(+) lymphocytes might be involved in the pathogenesis of PNH.
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November 2002

[Ex vivo expansion of CD34(+) CD59(+) cells from bone marrow of paroxysmal nocturnal hemoglobinuria patients].

Zhonghua Xue Ye Xue Za Zhi 2002 Nov;23(11):568-70

Department of Hematology, Pekin Union Medical College Hospital, CAMS and PUMC, Beijing 100730, China.

Objective: To study the separation, purification and ex vivo expansion of CD(34)(+) CD(59)(+) cells from patients with paroxysmal nocturnal hemoglobinuria (PNH), and explore the new treatment for the PNH patients.

Methods: CD(34)(+) CD(59)(+) cells were selected from the bone marrow mononuclear cells of PNH patients by means of immunomagnetic microbead-flow cytometry two step sorting method, followed by ex vivo expansion of the cells with combination of hematopoietic factors for two weeks.

Results: The best combination for the ex vivo expansion was SCF + IL-3 + IL-6 + FL + Tpo + Epo, and the seventh day was the most suitable time for the best harvest when the CD(34)(+) CD(59)(+) cells were 22.42 +/- 3.73 fold expanded. After ex vivo expansion, the cells remained CD(59) positive and potent capacity of colony formation, but their potentialities to multilineage differentiation were decreased.

Conclusion: The present study shows that ex vivo expansion of CD(34)(+) CD(59)(+) cells from PNH patients might promise the possibility of performing ABMT or APBSCT clinincally for the patients.
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November 2002

[Proliferative capacity of the isolated single CD(34)(+) glycosylphosphatidylinesitol-anchored (GPI) protein negative and positive hematopoietic cells in paroxysmal nocturnal hemoglobinuria].

Zhonghua Xue Ye Xue Za Zhi 2002 May;23(5):233-5

Department of Hematology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.

Objectives: To investigate the stroma-independent growth ability, multilineage differentiation and expansion of the single hematopoietic stem/progenitor cell from patients with paroxysmal nocturnal hematoglobinuria (PNH).

Method: The CD(34)(+) CD(59)(+) and CD(34)(+) CD(59)(-) cells from PNH patients and CD(34)(+) CD(59)(+) cells from normal volunteers were sorted as each single cell into a well of 96 well culture plates containing culture medium supplemented with SCF, IL-3, Epo, GM-CSF, G-CSF, IL-6, Tpo and Flt-3 ligand.

Results: (1) Single PNH CD(34)(+) CD(59)(-) cell had a higher capacities for plating efficiency, colony (>/= 50 cells) formation and cell expansion than that of the PNH CD(34)(+) CD(59)(+) cells (P < 0.05); (2) Both the single CD(34)(+) CD(59)(-) cells from PNH patients and the single CD(34)(+) CD(59)(+) cells from normal controls had similar capacities for cell plating efficiency and colony and large colony formation. The PNH CD(34)(+) CD(59)(-) cells had a lower average cell production and cell expansion capacity. (3) The single CD(34)(+) CD(59)(+) cells from both PNH patients and normal controls showed the same capacities for cell plating efficiency and colony formation. The PNH CD(34)(+) CD(59)(+) cells exhibited much lower capacity for large colony formation, average cell production and total cell expansion. (4) A diminished secondary colony formation ability was also observed in the PNH CD(34)(+) CD(59)(+) and CD(34)(-) CD(59)(-) clones.

Conclusion: The single PNH CD(34)(+) CD(59)(-) cells had growth advantage over the single PNH CD(34)(+) CD(59)(+) cells to some extent, but they both had impaired growth abilities as compared with CD(34)(+) cells from normal volunteers.
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May 2002

[Expression of CD(59) and CD(45) on lymphocytes in paroxysmal nocturnal hemoglobinuria patients].

Zhonghua Xue Ye Xue Za Zhi 2002 Feb;23(2):77-9

Department of Clinical Laboratory, Peking Union Medical College Hospital, CAMS & PUMC, Beijing 100730, China.

Objective: To analyse the expression of glycosylphosphatidylinositol (GPI)-anchored protein and functional transition of lymphocyte subsets in paroxysmal nocturnal hemoglobinuria (PNH) patients.

Methods: Flow cytometer and multi-color McAbs were used to detect CD(59) expression on lymphocytes and their subsets. Stimulation test was used to examine the ability of affected T cells in converting from CD(45RA) phenotype to CD(45RO) phenotype.

Results: In 14 PNH samples, CD(59) expression on affected lymphocytes was at a lower percentage than that on granulocytes and erythrocytes. B lymphocytes were widely affected than NK or T cells. CD(59)(-) deficient B lymphocytes existed in all PNH patients. More CD(8)(+) T cells were affected than CD(4)(+) T cells. Expression of HLA-DR and CD(45RO) on affected T cells were significantly lower than that on normal T cells (P < 0.01), whereas the expression of CD(45RA) was much higher on the former (P < 0.01). After stimulation with phytohemagglutinin (PHA), the expression of CD(45RA)(+) on affected T cells decreased sharply (the median value was from 82.7% to 26.6%), while the expression of CD(45RO)(+) increased significantly (the median value was from 19.6% to 64.3%).

Conclusions: The deficient CD(59) phenotype does involve in the different types of lymphocytes in PNH patients. Therefore affected B cells could be a good marker for diagnosis of PNH. The affected T cells were mainly comprised naive cells (CD(45RA)(+)HLA-DR(-)), which could acquire the memory phenotype (CD(45RO)(+)HLA-DR(+)) after stimulated by antigen.
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February 2002

[Composition analysis of hematopoietic stem/progenitor cells in peripheral blood and bone marrow from patients with paroxysmal nocturnal hemoglobinuria].

Zhonghua Xue Ye Xue Za Zhi 2002 Feb;23(2):73-6

Department of Hematology, Peking Union Medical College, Peking Union Medical College Hospital, CAMS & PUMC, Beijing 100730, China.

Objective: To evaluate the composition and proportion of the hematopoietic stem cells in peripheral blood (PB) and bone marrow (BM) in paroxysmal nocturnal hemoglobinuria (PNH) patients.

Methods: The CD(34) and CD(59) expressions in PB and BM samples from 21 PNH patients and 8 normal volunteers were analyzed by flow cytometry, and their total numbers were compared.

Results: The number of CD(34)(+) cells in both PB and BM of the patients was significantly lower than that in normal controls (P < 0.001), while there was no significant difference between the remittent patients and normal controls. The CD(34)(+) cells in PB of the patients exhibited predominantly normal CD(59) phenotype, which had no relationship either to the patients' clinical manifestations or to the CD(59) expression status of bone marrow CD(34)(+) cells.

Conclusion: The hematopoietic stem cells in patients with PNH decreases in number but show mainly normal phenotype in PB.
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February 2002