Publications by authors named "Zhihua Li"

470 Publications

Fabrication of a label-free electrochemical cell-based biosensor for toxicity assessment of thiram.

Chemosphere 2022 Aug 9:135960. Epub 2022 Aug 9.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, PR China; International Joint Research Laboratory of Intelligent Agriculture and Agri-products Processing, Jiangsu University, Zhenjiang, Jiangsu, 212013, PR China. Electronic address:

Thiram has been widely used in agriculture and may invades the food chain, posing a threat to human health. In this research, a label-free electrochemical cell-based biosensor was presented for in vitro toxicity assessment of thiram. HepG2 cells were cultured on [email protected] nano-flowers functionalized indium tin oxide coated glass electrode ([email protected]/ITO) to serve as biorecognition elements. AuNFs were electrodeposited on ITO to provide an enlarged specific surface area and benefited the output signal amplification. PLL was selected as an effective biocompatible coating material to facilitate cell adhesion and proliferation, thereby realizing one-step recording of electrochemical signals from thiram-treated cells. With the aid of the differential pulse voltammetry method, the fabricated biosensor was applied to assess the cytotoxicity of thiram. Results showed that the cytotoxicity measured by the fabricated biosensor exhibited a linear relationship related to thiram concentration ranging from 5 to 50 μM with a detection limit of 2.23 μM. The IC of thiram obtained by the biosensor was 29.5 μM, which was close to that of conventional MTT assay (30.8 μM). The effects of thiram on HepG2 cells were also investigated via SEM and flow cytometry. Meanwhile, the proposed biosensor was used to evaluate the toxicity of thiram in fruit samples. Results indicated that the toxicity of thiram cannot be ignored even at a low residual concentration in food (≤5 mg/kg). In conclusion, the developed sensor showed excellent sensitivity, stability, and reliability, which provided a great capacity for the convenient toxicity evaluation of thiram residue in food.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135960DOI Listing
August 2022

A Novel Gas Sensor for Detecting Pork Freshness Based on PANI/AgNWs/Silk.

Foods 2022 Aug 8;11(15). Epub 2022 Aug 8.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.

A novel, operational, reliable, flexible gas sensor based on silk fibroin fibers (SFFs) as a substrate was proposed for detecting the freshness of pork. Silk is one of the earliest animal fibers utilized by humans, and SFFs exposed many biological micromolecules on the surface. Thus, the gas sensor was fabricated through polyaniline (PANI) and silver nanowires (AgNWs) and deposited on SFFs by in-suit polymerization. With trimethylamine (TMA) as a model gas, the sensing properties of the PANI/AgNWs/silk composites were examined at room temperature, and the linear correlativity was very prominent between these sensing measures and the TMA measures in the range of 3.33 μg/L-1200 μg/L. When the pork sample is detected by the sensor, it can be classified into fresh or stale pork with the total volatile basic nitrogen (TVB-N) as an index. The result indicated that the gas sensor was effective and showed great potential for applications to detect the freshness of pork.
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http://dx.doi.org/10.3390/foods11152372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368743PMC
August 2022

Role of types and dosages of cations with low valance states on microalgal-bacterial symbiosis system treating wastewater.

Bioresour Technol 2022 Aug 6:127755. Epub 2022 Aug 6.

Shaanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China; International Science & Technology Cooperation Center for Urban Alternative Water Resources Development, Xi'an 710055, China.

This study investigated the roles of cations with low valance states, including Mg, K and Li, on microalgal-bacterial symbiosis (MABS) system treating wastewater. Results showed that Mg and K improved pollutants removal at dosages of less than 1 mM, and a further increase led to poorer performances. Conversely, Li inhibited pollutants removal. Mechanism study indicated Mg and K with dosages of 10 mM and Li inhibited the activities of MABS biomass (especially Chlorella), with bad absorption efficiencies of 20.64%, 13.65% and lower than 10%, leading to more extracellular polymeric substances production. Larger ions' charge density resulted in larger attraction of water molecules, contributing to the decreased distance between microalgae cells and increased biomass aggregation. Both these two impacts led to the order of impact degree on MABS aggregates: Mg > Li > K. The findings can present some new perspectives on assessing effects of cations on MABS system.
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http://dx.doi.org/10.1016/j.biortech.2022.127755DOI Listing
August 2022

Ratiometric Fluorescent Metal-Organic Framework Biosensor for Ultrasensitive Detection of Acrylamide.

J Agric Food Chem 2022 Aug 8;70(32):10065-10074. Epub 2022 Aug 8.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.

Acrylamide is a neurotoxin and carcinogen that forms during the thermal processing of food, inflicting irreversible harm to human health. Herein, a ratiometric fluorescence biosensor based on a 6-carboxyfluorescein-labeled aptamer (FAM-ssDNA) and porphyrin metal-organic framework (PCN-224) was developed. PCN-224 exhibits strong adsorption capacity for FAM-ssDNA and also quenches the fluorescence of FAM-ssDNA via fluorescence resonance energy transfer and photoinduced electron transfer. FAM-ssDNA hybridizes with complementary DNA to form double-stranded DNA (FAM-dsDNA), which is liberated from the PCN-224 surface, resulting in fluorescence recovery. However, the intrinsic fluorescence of the ligand remains unchanged. Acrylamide can create an adduct with FAM-ssDNA and inhibit the hybridization of FAM-dsDNA, thus realizing ratiometric sensing of acrylamide. The proposed biosensor displays excellent detection performance from 10 nM∼0.5 mM with a limit of detection of 1.9 nM. In conclusion, a fabricated biosensor was successfully applied to detect acrylamide in thermally processed food, and the results were consistent with those of high-performance liquid chromatography.
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http://dx.doi.org/10.1021/acs.jafc.2c04756DOI Listing
August 2022

Corrigendum to "LncRNA HOTTIP modulates cancer stem cell properties in human pancreatic cancer by regulating HOXA9" [Canc. Lett. 410 (2017) 68-81].

Cancer Lett 2022 Oct 5;545:215836. Epub 2022 Aug 5.

Department of Pancreaticobiliary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China; Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China. Electronic address:

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http://dx.doi.org/10.1016/j.canlet.2022.215836DOI Listing
October 2022

Cell Trajectory-Related Genes of Lung Adenocarcinoma Predict Tumor Immune Microenvironment and Prognosis of Patients.

Front Oncol 2022 18;12:911401. Epub 2022 Jul 18.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital with Nanjing Medical University (Jiangsu Province Hospital), Nanjing, China.

Background: Lung adenocarcinoma (LUAD) is the most common subtype of lung cancer which typically exhibits a diverse progression trajectory. Our study sought to explore the cell differentiation trajectory of LUAD and its clinical relevance.

Methods: Utilizing a single-cell RNA-sequencing dataset (GSE117570), we identified LUAD cells of distinct differential status along with differentiation-related genes (DRGs). DRGs were applied to the analysis of bulk-tissue RNA-sequencing dataset (GSE72094) to classify tumors into different subtypes, whose clinical relevance was further analyzed. DRGs were also applied to gene co-expression network analysis (WGCNA) using another bulk-tissue RNA-sequencing dataset (TCGA-LUAD). Genes from modules that demonstrated a significant correlation with clinical traits and were differentially expressed between normal tissue and tumors were identified. Among these, genes with significant prognostic relevance were used for the development of a prognostic nomogram, which was tested on TCGA-LUAD dataset and validated in GSE72094. Finally, CCK-8, EdU, cell apoptosis, cell colony formation, and Transwell assays were used to verify the functions of the identified genes.

Results: Four clusters of cells with distinct differentiation status were characterized, whose DRGs were predominantly correlated with pathways of immune regulation. Based on DRGs, tumors could be clustered into four subtypes associated with distinct immune microenvironment and clinical outcomes. DRGs were categorized into four modules. A total of nine DRGs (, , , , , , , , and ) with most significant survival-predicting power were integrated to develop a prognostic model, which outperformed the traditional parameters in predicting clinical outcomes. Finally, we verified that knockdown of WFDC2 inhibited proliferation, migration, and invasion but promoted the apoptosis of A549 cells .

Conclusion: The cellular composition and cellular differentiation status of tumor mass can predict the clinical outcomes of LUAD patients. It also plays an important role in shaping the tumor immune microenvironment.
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http://dx.doi.org/10.3389/fonc.2022.911401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339705PMC
July 2022

Functionalization of Electrospun Nanofiber for Bone Tissue Engineering.

Polymers (Basel) 2022 Jul 20;14(14). Epub 2022 Jul 20.

School of Stomatology, Nanchang University, Nanchang 330006, China.

Bone-tissue engineering is an alternative treatment for bone defects with great potential in which scaffold is a critical factor to determine the effect of bone regeneration. Electrospun nanofibers are widely used as scaffolds in the biomedical field for their similarity with the structure of the extracellular matrix (ECM). Their unique characteristics are: larger surface areas, porosity and processability; these make them ideal candidates for bone-tissue engineering. This review briefly introduces bone-tissue engineering and summarizes the materials and methods for electrospining. More importantly, how to functionalize electrospun nanofibers to make them more conducive for bone regeneration is highlighted. Finally, the existing deficiencies of functionalized electrospun nanofibers for promoting osteogenesis are proposed. Such a summary can lay the foundation for the clinical practice of functionalized electrospun nanofibers.
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http://dx.doi.org/10.3390/polym14142940DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318783PMC
July 2022

Pyeloplasty with the Kangduo surgical robot vs. the da Vinci Si robotic system: preliminary results.

J Endourol 2022 Jul 21. Epub 2022 Jul 21.

Beijing, China;

Objective: To share our experience in robot-assisted pyeloplasty (RAP) with the Kangduo (KD) surgical robot vs. the da Vinci Si (DV) robotic system (KD-RAP vs. DV-RAP, respectively).

Methods: From August 2019 to February 2021, 16 patients with ureteropelvic junction obstruction (UPJO) underwent KD-RAP and other 16 patients with UPJO accepted DV-RAP. All procedures were performed by the same surgeon. The perioperative results and follow-up data were prospectively collected and compared.

Results: There was no conversion to open or laparoscopic surgery. The mean operation time was significantly longer in the KD-RAP group than the DV-RAP group (141 ± 28 vs. 118 ± 31 min, respectively, p = 0.04). The time per stitch was significantly longer in the KD-RAP group than the DV-RAP group (1.7 ± 0.5 vs. 1.4 ± 0.3 min, respectively, p = 0.05). No significant difference was noted in the estimated blood loss and the postoperative length of hospitalization. At a median follow-up of 19 (range, 17-21) and 19.5 (range, 14-33) months for the KD-RAP and DV-RAP groups respectively, no difference was noted in the success rates between the KD-RAP and DV-RAP groups (93.75% and 100%, respectively; p = 0.31). Complications were comparable between the two groups (p = 0.54). One (6.3%) patient developed urinary infection which responded well to oral antibiotics in KD-RAP group and 2 (12.5%) patients suffered from irritation symptoms of bladder which improved after removal of D-J stent in DV-RAP group.

Conclusion: The RAP with the use of the KD system was feasible, safe and effective. The DV-RAP group showed advantage in the operation time and the time per stitch.
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http://dx.doi.org/10.1089/end.2022.0366DOI Listing
July 2022

Hydrogen sulfide gas sensing toward on-site monitoring of chilled meat spoilage based on ratio-type fluorescent probe.

Food Chem 2022 Jul 9;396:133654. Epub 2022 Jul 9.

School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd, 212013 Zhenjiang, Jiangsu, China; International Joint Research Laboratory of Intelligent Agriculture and Agri-products Processing (Jiangsu University), Jiangsu Education Department, China. Electronic address:

In this study, a fluorescence sensing platform for visual detection of hydrogen sulfide (HS) based on ratiometric fluorescent substances was developed to real-time monitor meat spoilage. The copper nanoclusters (CuNCs) and nitrogen-doped carbon quantum dots (CNQDs) were used as dual emission fluorescence materials. The fluorescence ratio of the two wavelengths decreased in the sulphide concentration range of 0-3 μmolL(exp), with a detection limit of 62.7 nmolL(exp). In order to capture hydrogen sulfide gas in the air, the ratio fluorescent material is loaded on the paper base. By processing the RGB value of the photo under UV light, the detection limit of the sensor is 4.35 ppt in the range of 0 ∼ 45.2 ppt HS gas concentration. This portable visual analysis greatly simplifies the steps of HS gas detection while ensuring sensor stability and sensitivity. It also provides a new method for HS detection during the meat storage process.
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http://dx.doi.org/10.1016/j.foodchem.2022.133654DOI Listing
July 2022

Preoperative chemoradiotherapy with capecitabine and triweekly oxaliplatin versus capecitabine monotherapy for locally advanced rectal cancer: a propensity-score matched study.

BMC Cancer 2022 Jul 18;22(1):789. Epub 2022 Jul 18.

Department of Radiation Oncology, Fujian Medical University Union Hospital, Xinquan Road 29, Fuzhou, 350001, China.

Background: Distant metastasis has been the main failure pattern for locoregionally advanced rectal cancer (LARC) patients, and intensified neoadjuvant chemotherapy has become a popular research topic. The present study aimed to compare the survival outcomes, acute toxicities and surgical complications in LARC patients who received preoperative chemoradiotherapy with triweekly oxaliplatin and capecitabine (triweekly XELOX) or capecitabine.

Methods: Between 2007 and 2017, patients with clinically staged II-III rectal cancer who were treated with preoperative chemoradiotherapy using either triweekly XELOX (oxaliplatin 130 mg/m plus capecitabine 825 mg/m) or capecitabine were included. Variables potentially influencing chemotherapy treatment selection were used to generate propensity scores (PS). The association between chemotherapy regimens and survival endpoints, including distant metastasis-free survival (DMFS), overall survival (OS) and disease-free survival (DFS), were evaluated and adjusted with PS. The acute toxicities and surgical complications were also compared.

Results: A total of 810 patients were included in the analysis; 277 (34.2%) patients received triweekly XELOX, and 533 (65.8%) received capecitabine. The pathological complete response (pCR) rates were 20.2 and 19.9% (P = 0.912) for the groups treated with triweekly XELOX and capecitabine, respectively. The 5-year DMFS, OS and DFS with triweekly XELOX versus capecitabine were 75.6% vs. 77.6% (P = 0.555), 79.2% vs. 83.3% (P = 0.101), and 69.9% vs. 73.7% (P = 0.283), respectively. Triweekly XELOX was not associated with an increased risk of severe toxicity during chemoradiotherapy, but it increased the risk of postoperative complications compared to capecitabine. After PS adjustment, the differences between the two groups remained insignificant in pCR rate, survival outcomes, and acute toxicities, and the difference in surgical complications disappeared.

Conclusions: Triweekly XELOX or capecitabine concurrent with neoadjuvant radiotherapy leads to similar long-term survival outcomes, acute toxicities and surgical complications in LARC patients.
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http://dx.doi.org/10.1186/s12885-022-09855-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295262PMC
July 2022

Circulating tumor DNA detection in peripheral blood in postoperative efficacy evaluation and recurrence risk prediction of lung cancer.

Cell Mol Biol (Noisy-le-grand) 2022 Feb 4;67(5):51-56. Epub 2022 Feb 4.

Department of Thoracic Surgery, Shenzhen Longgang Central Hospital, Shenzhen, 518116, China.

This study mainly explores the application in the evaluation of postoperative efficacy and recurrence risk prediction. In this study, 50 lung cancer patients treated with thoracic surgery were selected. Collect the peripheral blood of these patients before the operation, separate the blood sample from the plasma by high-speed centrifugal separation, extract the DNA in the plasma by the magnetic bead method, and then extract the ctDNA. A liquid-phase chip capture system is used to highly concentrate target DNA. We use the IlluminaHiseq platform to sort with high throughput and high depth. As an indicator of ctDNA detection and analysis, the AF value of gene mutation frequency is used for calibration. The sensitivity of CEA detection in peripheral blood of tumor patients (TP) is 30% (15/50), and the sensitivity of ctDNA detection is significantly higher than that of CEA detection. Research results show that the number.
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http://dx.doi.org/10.14715/cmb/2021.67.5.7DOI Listing
February 2022

Application of Protein in Extrusion-Based 3D Food Printing: Current Status and Prospectus.

Foods 2022 Jun 27;11(13). Epub 2022 Jun 27.

Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.

Extrusion-based 3D food printing is one of the most common ways to manufacture complex shapes and personalized food. A wide variety of food raw materials have been documented in the last two decades for the fabrication of personalized food for various groups of people. This review aims to highlight the most relevant and current information on the use of protein raw materials as functional 3D food printing ink. The functional properties of protein raw materials, influencing factors, and application of different types of protein in 3D food printing were also discussed. This article also clarified that the effective and reasonable utilization of protein is a vital part of the future 3D food printing ink development process. The challenges of achieving comprehensive nutrition and customization, enhancing printing precision and accuracy, and paying attention to product appearance, texture, and shelf life remain significant.
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http://dx.doi.org/10.3390/foods11131902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265415PMC
June 2022

Easy-to-Use Visual Sensing System for Milk Freshness, Sensitized with Acidity-Responsive N-Doped Carbon Quantum Dots.

Foods 2022 Jun 23;11(13). Epub 2022 Jun 23.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.

This study established a flexible and eye-readable sensing system for the easy-to-use, visual detection of milk freshness, using acidity-responsive N-doped carbon quantum dots (N-CQDs). N-CQDs, rich in amino groups and with characteristic acidity sensitivity, exhibited high relative quantum yields of 25.2% and an optimal emission wavelength of 567 nm. The N-CQDs fluorescence quenching upon the dissociated hydrogen ions (H) in milk and their reacting with the amino groups produced an excellent linear relation (R = 0.996) between the fluorescence intensity and the milk acidity, which indicated that the fluorescence of the N-CQDs was highly correlated with milk freshness. Furthermore, a fluorescence sensor was designed by depositing the N-CQDs on filter-papers and starch-gel films, to provide eye-readable signals under UV light. A fluorescence colorimetric card was developed, based on the decrease in fluorescence brightness as freshness deteriorated. With the advantages of high sensitivity and eye readability, the proposed sensor could detect spoiled milk in advance and without any preprocessing steps, offering a promising method of assessing food safety.
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http://dx.doi.org/10.3390/foods11131855DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265914PMC
June 2022

Ileal ureteral replacement for the management of ureteral avulsion during ureteroscopic lithotripsy: a case series.

BMC Surg 2022 Jul 7;22(1):262. Epub 2022 Jul 7.

Department of Urology, Peking University First Hospital, National Urological Cancer Center, Institute of Urology, Peking University, No. 8 Xishiku St, Xicheng District, Beijing, 100034, China.

Introduction: To describe our initial experience with ileal ureteral replacement (IUR) for the management of ureteral avulsion (UA) during ureteroscopic lithotripsy.

Methods: Between September 2010 and April 2021, ten patients received ileal ureteral replacement for ureteral avulsion during ureteroscopic lithotripsy. Anterograde urography and computed tomography urography (CTU) were applied to evaluate the lesion. Follow-up was performed with magnetic resonance urography and renal ultrasound as well as clinical assessment of symptoms. We retrospectively analysed the clinical data of ten patients treated with ileal ureteral replacement for the treatment of ureteral avulsion.

Results: Four patients underwent open ileal ureteral replacement, two underwent laparoscopic ileal ureteral replacement, and four underwent robotic-assisted ileal ureteral replacement. The mean operative time (OT) was 310 min (range 191-530). The mean estimated blood loss (EBL) was 193 mL (range 10-1000). The mean length of the ileal graft was 21 cm (range 12-25). The median postoperative hospital time was 13 days (range 7-19). All surgeries were effectively completed, and no case required open conversion in laparoscopic and robotic-assisted surgeries. There was no obvious hydronephrosis according to contrast-enhanced computed tomography 3-dimensional reconstruction images without serious complications or progressive hydronephrosis during a median follow-up duration of 51 months (range 5-131), and the success rate was 100%.

Conclusions: Our initial results and experience showed that ileal ureteral replacement for the management of ureteral avulsion during ureteroscopic lithotripsy is safe and feasible.
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http://dx.doi.org/10.1186/s12893-022-01690-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264655PMC
July 2022

Development of a High-Coverage Quantitative Metabolome Analysis Method Using Four-Channel Chemical Isotope Labeling LC-MS for Analyzing High-Salt Fermented Food.

J Agric Food Chem 2022 Jul 5;70(28):8827-8837. Epub 2022 Jul 5.

The Metabolomics Innovation Centre and Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.

Metabolome analysis of high-salt fermented food can be an analytical challenge, as the salts can interfere with the sample processing and analysis. In this work, we describe a four-channel chemical isotope labeling (CIL) LC-MS approach for a comprehensive metabolome analysis of high-salt fermented food. The workflow includes metabolite extraction, chemical labeling of metabolites using dansyl chloride, dansylhydrazine, or -dimethylaminophenacyl bromide reagents to enhance separation and ionization, LC-UV measurement of the total concentration of dansyl-labeled metabolites in each sample for sample normalization, mixing of C- and C-reagent-labeled samples, high-resolution LC-MS analysis, and data processing. Metabolome analysis of fermented foods, including fermented red pepper (FRP) sauce, soy sauce, and sufu (a fermented soybean food), showed unprecedented high metabolic coverage. Metabolome comparison of FRP, soy sauce, and sufu, as well as soy sauce and sufu, indicated great diversity of metabolite types and abundances in these foods. In addition, we analyzed two groups of samples of the same type, FRP with 10% (w/w) and 15% (w/w) salt contents, and detected large variations in multiple categories of metabolites belonging to a number of different metabolic pathways. We envisage that this CIL LC-MS approach can be generally used for metabolomic studies of high-salt fermented food. CIL LC-MS allows high-coverage identification and quantification that could not be done using other methods.
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http://dx.doi.org/10.1021/acs.jafc.2c03481DOI Listing
July 2022

Transcriptomic analysis of starch accumulation patterns in different glutinous sorghum seeds.

Sci Rep 2022 Jul 1;12(1):11133. Epub 2022 Jul 1.

Sorghum Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, 110161, Liaoning Province, People's Republic of China.

Sorghum is a high-quality raw material for brewing white wine, and the starch content in seeds has a large impact on brewing quality. Transcriptomic data obtained from a glutinous variety (Liaonian3) and a non-glutinous variety (Liaoza10) at 3, 18, and 30 days after pollination were analyzed to identify genes associated with starch accumulation. The amylopectin content was significantly higher in Liaonian3 compared to Liaoza10, but the amylose content and total starch content were lower. There were 6634 differentially expressed genes found in Liaoza10 between 3 and 18 d after pollination, and 779 differentially expressed genes between 18 and 30 d after pollination. In Liaonian3, there were 6768 differentially expressed genes between 3 and 18 d after pollination, and 7630 differentially expressed genes between 18 and 30 d after pollination. Genes were grouped by expression profiles over the three time points and the profiles were analyzed for enrichment of gene ontology terms and biochemical pathways. Profile 1 (decreasing expression from 3 to 30 d) for Liaoza10 was enriched in ribosomes, metabolic pathways, and carbon metabolic pathways. Profile 0 (decreasing expression from 3 to 18 d and consistent expression from 18 to 30 d) was enriched in pathways related to sugar or starch metabolism. Although the starch accumulation rate in Liaonian3 and Liaoza10 showed a profile of increasing and then decreasing, the expression of genes related to starch synthesis gradually decreased with time since pollination, demonstrating the complexity of starch synthesis. According to orthologous gene alignment and expression analysis, 19 genes such as entrzID_8068390 and entrzID_8066807 were found to be the key genes for starch synthesis and glutinous and non-glutinous differentiation in sorghum grains.
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http://dx.doi.org/10.1038/s41598-022-15394-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249802PMC
July 2022

CRISPR-Cas9 Toolkit for Genome Editing in an Autotrophic CO-Fixing Methanogenic Archaeon.

Microbiol Spectr 2022 Jun 29:e0116522. Epub 2022 Jun 29.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

The CRISPR-Cas9 system is a robust genome editing tool that is widely applied in eukaryotes and bacteria. However, use of this technique has only been developed for one species of , a domain of life ranking in parallel with and . In this study, we applied the CRISPR-Cas9 genome editing technique to Methanococcus maripaludis, an autotrophic and hydrogenotrophic methanogenic archaeon with a remarkably polyploid genome comprising up to ~55 chromosomal copies per cell. An editing plasmid was designed that encodes small guide RNA (sgRNA), Cas9 protein and an ~1-kb repair template (donor). Highly efficient (75% to 100%) and precise genome editing was achieved following one-step transformation. Significantly, the Cas9-based system efficiently deleted one or two genes and a large DNA fragment (~9 kb) and even synchronously deleted 13 genes located at three loci in all chromosomal copies of . Moreover, precise genome modifications, such as gene tagging and multiple- and even single-nucleotide mutagenesis, were also introduced with high efficiency. Further, as a proof of concept, precise mutagenesis at the nucleotide level allowed the engineering of both transcriptional and translational activities. Mutations were introduced into an archaeal promoter BRE (transcription factor B [TFB] recognition element), a terminator U-tract region, and a gene coding region. Stop codon introduction into a gene through single-nucleotide substitution shut down its expression, providing an alternative strategy for gene inactivation. In conclusion, the robust CRISPR-Cas9 genetic toolkit developed in this investigation greatly facilitates the application of as a model system in the study of archaeal biology and biotechnology development, particularly CO-based biotechnologies. Archaea are prokaryotes with intriguing biological characteristics. They possess bacterial cell structures but eukaryotic homologous information processing machinery and eukaryotic featured proteins. Archaea also display excellent adaptability to extreme environments and play pivotal roles in ecological processes, thus exhibiting valuable biotechnological potential. However, the in-depth understanding and practical application of archaea are much lagging, because only a minority of pure cultures are available, and even worse, very few can be genetically manipulated. This work developed CRISPR-Cas9-based genome editing technology in Methanococcus maripaludis, a CO-fixing methanogenic archaeon. The CRISPR-Cas9 approach developed in this study provides an elegant and efficient genome editing toolkit that can be applied in the knockout of single or multiple genes, gene tagging, multiple- or single-nucleotide mutagenesis, and inactivation of gene expression by introduction of stop codons. The successful development of the CRISPR-Cas9 toolkit will facilitate the application of in archaeal biology research and biotechnology development, particularly CO-derived biotechnologies.
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http://dx.doi.org/10.1128/spectrum.01165-22DOI Listing
June 2022

Development of a Translational Model to Assess the Impact of Opioid Overdose and Naloxone Dosing on Respiratory Depression and Cardiac Arrest.

Clin Pharmacol Ther 2022 Jun 29. Epub 2022 Jun 29.

Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.

In response to a surge of deaths from synthetic opioid overdoses, there have been increased efforts to distribute naloxone products in community settings. Prior research has assessed the effectiveness of naloxone in the hospital setting; however, it is challenging to assess naloxone dosing regimens in the community/first-responder setting, including reversal of respiratory depression effects of fentanyl and its derivatives (fentanyls). Here, we describe the development and validation of a mechanistic model that combines opioid mu receptor binding kinetics, opioid agonist and antagonist pharmacokinetics, and human respiratory and circulatory physiology, to evaluate naloxone dosing to reverse respiratory depression. Validation supports our model, which can quantitatively predict displacement of opioids by naloxone from opioid mu receptors in vitro, hypoxia-induced cardiac arrest in vivo, and opioid-induced respiratory depression in humans from different fentanyls. After validation, overdose simulations were performed with fentanyl and carfentanil followed by administration of different intramuscular naloxone products. Carfentanil induced more cardiac arrest events and was more difficult to reverse than fentanyl. Opioid receptor binding data indicated that carfentanil has substantially slower dissociation kinetics from the opioid receptor compared with nine other fentanyls tested, which likely contributes to the difficulty in reversing carfentanil. Administration of the same dose of naloxone intramuscularly from two different naloxone products with different formulations resulted in differences in the number of virtual patients experiencing cardiac arrest. This work provides a robust framework to evaluate dosing regimens of opioid receptor antagonists to reverse opioid-induced respiratory depression, including those caused by newly emerging synthetic opioids.
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http://dx.doi.org/10.1002/cpt.2696DOI Listing
June 2022

High- sensitivity bilayer nanofiber film based on polyvinyl alcohol/sodium alginate/polyvinylidene fluoride for pork spoilage visual monitoring and preservation.

Food Chem 2022 Nov 8;394:133439. Epub 2022 Jun 8.

Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China. Electronic address:

A colorimetric bilayer film for pork freshness detection and preservation was developed using electrospinning technique. The bilayer film consisted of a layer with polyvinyl alcohol - sodium alginate - alizarin as sensor layer and a layer with polyvinylidene fluoride - vanillin as antibacterial layer. The water contact angle of bilayer film was larger than the single colorimetric layer. The color sensitivity to the ammonia of the bilayer film was higher, with an ΔE value of 47.99. The film could display color shifts from yellow to purple with the naked eye is critical for checking pork freshness. In addition, the bilayer film exhibited sensitive antibacterial activity, with an inhibition zone against S. aureus (8.3 mm) and E. coli (14.7 mm), respectively. Finally, the bilayer film was applied to freshness monitoring of pork. The film displayed significant color changes and prolonged the pork shelf life by 24 h at 25 °C.
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http://dx.doi.org/10.1016/j.foodchem.2022.133439DOI Listing
November 2022

Ocular Adverse Events after Inactivated COVID-19 Vaccination.

Vaccines (Basel) 2022 Jun 9;10(6). Epub 2022 Jun 9.

Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, 17 Hougou Lane, Chongnei Street, Beijing 100005, China.

Purpose: To report the clinical characteristics of ocular adverse events that have occurred, in China, after vaccination with inactivated COVID-19 vaccines.

Methods: A retrospective cross-sectional observational study was conducted of ocular disorders that occurred within 15 days from any dose of an inactivated COVID-19 vaccine. Information on gender, age, the interval between the vaccination and ocular symptoms, laterality, duration of the ocular symptoms, primary visual acuity, and clinical diagnosis were retrospectively collected.

Results: Twenty-four patients were involved in the study, including 15 females and 9 males, with a mean age of 41 ± 16 years (range of 8-71 years). The patients all denied a prior history of COVID-19 infection. Ocular adverse events occurred after the first dose of vaccine in 18 patients and, after the second or third doses, in six patients. The interval between vaccination with the inactivated COVID-19 vaccine and ocular symptoms was 6 ± 5 days; six patients were bilaterally involved and 18 patients were unilaterally involved. Regarding the diagnosis, 10 patients were diagnosed with white dot syndrome (WDS), 9 patients were diagnosed with uveitis, and 5 patients were diagnosed with retinal vascular disorders. The ages of patients with WDS were younger than those with uveitis or retinal vascular disorders (32 ± 10 vs. 48 ± 18, < 0.05). For patients diagnosed with WDS, the best-corrected visual acuity (BCVA) was 0.74 ± 0.73 LogMAR. For patients diagnosed with retinal vascular disorders or uveitis, the BCVA was 1.44 ± 1.26 LogMAR. There was no significant difference ( > 0.05).

Conclusions: A relationship cannot be established between inactivated COVID-19 vaccines and ocular disorders; therefore, further investigation of the clinical spectrum of ocular adverse events after vaccination with an inactivated COVID-19 vaccine is necessary.
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http://dx.doi.org/10.3390/vaccines10060918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230105PMC
June 2022

A Review on Rainfall Measurement Based on Commercial Microwave Links in Wireless Cellular Networks.

Sensors (Basel) 2022 Jun 10;22(12). Epub 2022 Jun 10.

School of Information and Electrical Engineering, Hebei University of Engineering, Handan 056038, China.

As one of the most critical elements in the hydrological cycle, real-time and accurate rainfall measurement is of great significance to flood and drought disaster risk assessment and early warning. Using commercial microwave links (CMLs) to conduct rainfall measure is a promising solution due to the advantages of high spatial resolution, low implementation cost, near-surface measurement, and so on. However, because of the temporal and spatial dynamics of rainfall and the atmospheric influence, it is necessary to go through complicated signal processing steps from signal attenuation analysis of a CML to rainfall map. This article first introduces the basic principle and the revolution of CML-based rainfall measurement. Then, the article illustrates different steps of signal process in CML-based rainfall measurement, reviewing the state of the art solutions in each step. In addition, uncertainties and errors involved in each step of signal process as well as their impacts on the accuracy of rainfall measurement are analyzed. Moreover, the article also discusses how machine learning technologies facilitate CML-based rainfall measurement. Additionally, the applications of CML in monitoring phenomena other than rain and the hydrological simulation are summarized. Finally, the challenges and future directions are discussed.
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http://dx.doi.org/10.3390/s22124395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230635PMC
June 2022

Shade Delayed Flowering Phenology and Decreased Reproductive Growth of L.

Front Plant Sci 2022 2;13:835380. Epub 2022 Jun 2.

College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, China.

Alfalfa ( L.) is an important forage in intercropping or rotation ecosystem, and shading is the principal limiting factor for its growth under the crop or forest. Agronomic studies showed that shading would systematically reduce the biomass of alfalfa. However, little is known about the reproduction of alfalfa under shading conditions. In order to study the effect of shading on the reproductive characteristics of alfalfa, two alfalfa cultivars ("Victoria" and "Eureka") were used to study the effect of shading levels (full light, 56.4% shade, and 78.7% shade) on alfalfa flowering phenology, pollen viability, stigma receptivity, and seed quality. Results showed that shading delayed flowering phenology, shortened the flowering stage, faded the flower colors, and significantly reduced pollen viability, stigma receptivity, the number of flowers, quantity, and quality of seeds. Under shading conditions, seed yield per plant was obviously positively correlated with germination potential, germination rate, pollen viability, and 1,000-seed weight. The number of flower buds, pollen viability, 1,000-seed weight, and germination rate had the greatest positive direct impact on seed yield per plant. Our findings suggested that delayed flowering and reducing reproduction growth were important strategies for alfalfa to cope with shading and pollen viability was the key bottleneck for the success of alfalfa reproduction under shading. However, given that alfalfa is a perennial vegetative-harvest forage, delaying flowering in a weak light environment was beneficial to maintain the high aboveground biomass of alfalfa. Therefore, this should be taken into account when breeding alfalfa cultivars suitable for intercropping. Future research should further reveal the genetic and molecular mechanism of delayed flowering regulating the accumulation and distribution of assimilates between vegetative and reproductive organs of alfalfa under shading, so as to provide a theoretical basis for breeding of shade-tolerant alfalfa cultivars.
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http://dx.doi.org/10.3389/fpls.2022.835380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203126PMC
June 2022

Therapeutic potential of anti-miR29a in breast cancer patients with type 2 diabetes: an and xenograft mouse-model study.

Transl Cancer Res 2022 May;11(5):1285-1296

Key Laboratory of Breast Diseases in Jiangxi Province, Third Hospital of Nanchang, Nanchang, China.

Background: MicroRNAs (miRNAs) acting as tumour suppressors or oncogenes, known as oncomiRs, are a promising new focus in targeted therapies for cancer. Approximately 16% of breast cancer patients have pre-existing diabetes. Breast cancer with type 2 diabetes mellitus (BDM) is provided with its unique biological characteristics and clinical characteristics. This study primarily investigated the therapeutic potential and regulatory mechanism of miR-29a in patients with BDM.

Methods: The significance of miR-29a in BDM was analyzed by real-time reverse transcriptase polymerase chain reaction (qRT-PCR) in breast tissues. A cell model for BDM was established by using MDA-MB-231 cells cultured in 3T3-L1 adipocytes cultured with high levels of glucose and insulin. A type 2 diabetes mellitus (T2DM) mouse model was induced in female BALB/c mice through a high-fat diet plus low doses of streptozotocin (STZ). The xenograft mouse-model for BDM was established on these T2DM mouse by using MDA-MB-231 cells. Then the biological effects of miR-29a knockdown mediated by lentivirus-shRNAs on cell proliferation, apoptosis, cell cycle, and migration were investigated.

Results: Our results indicated that miR-29a was upregulated in patients with BDM, which correlated with a worse prognosis. In human breast cancer cells, miR-29a knockdown reduced cell proliferation and cell migration and invasion in BDM. In the T2DM xenograft, miR-29a knockdown suppressed MDA-MB-231 cells tumorigenesis and metastasis. We also demonstrated that miR-29a promoted BDM cell growth and metastasis by targeting Sirtuin 1 (SIRT1).

Conclusions: Our findings indicated that anti-miR-29a inhibited cell proliferation and invasion in BDM by targeting SIRT1. We believe anti-miR-29a may represent a novel therapeutic approach for the management of patients with BDM.
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http://dx.doi.org/10.21037/tcr-22-824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189168PMC
May 2022

Calibration and Validation of a Mechanistic COVID-19 Model for Translational Quantitative Systems Pharmacology - A Proof-of-Concept Model Development for Remdesivir.

Clin Pharmacol Ther 2022 Jun 13. Epub 2022 Jun 13.

Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.

With the ongoing global pandemic of coronavirus disease 2019 (COVID-19), there is an urgent need to accelerate the traditional drug development process. Many studies identified potential COVID-19 therapies based on promising nonclinical data. However, the poor translatability from nonclinical to clinical settings has led to failures of many of these drug candidates in the clinical phase. In this study, we propose a mechanism-based, quantitative framework to translate nonclinical findings to clinical outcome. Adopting a modularized approach, this framework includes an in silico disease model for COVID-19 (virus infection and human immune responses) and a pharmacological component for COVID-19 therapies. The disease model was able to reproduce important longitudinal clinical data for patients with mild and severe COVID-19, including viral titer, key immunological cytokines, antibody responses, and time courses of lymphopenia. Using remdesivir as a proof-of-concept example of model development for the pharmacological component, we developed a pharmacological model that describes the conversion of intravenously administered remdesivir as a prodrug to its active metabolite nucleoside triphosphate through intracellular metabolism and connected it to the COVID-19 disease model. After being calibrated with the placebo arm data, our model was independently and quantitatively able to predict the primary endpoint (time to recovery) of the remdesivir clinical study, Adaptive Covid-19 Clinical Trial (ACTT). Our work demonstrates the possibility of quantitatively predicting clinical outcome based on nonclinical data and mechanistic understanding of the disease and provides a modularized framework to aid in candidate drug selection and clinical trial design for COVID-19 therapeutics.
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http://dx.doi.org/10.1002/cpt.2686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9349538PMC
June 2022

Correction: lncRNA-PLACT1 sustains activation of NF-κB pathway through a positive feedback loop with IκBα/E2F1 axis in pancreatic cancer.

Mol Cancer 2022 Jun 10;21(1):128. Epub 2022 Jun 10.

Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, 106th of 2nd Zhongshan Road, Yuexiu District, Guangzhou, Guangdong Province, 510080, People's Republic of China.

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http://dx.doi.org/10.1186/s12943-022-01585-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188170PMC
June 2022

A novel approach to estimate and control denitrification performance in activated sludge systems with respirogram technology.

J Environ Sci (China) 2022 Nov 1;121:112-121. Epub 2022 Feb 1.

Shanghai Environmental & Sanitary Engineering Design Institute Co. Ltd., Shanghai 200232, China.

Respirogram technology has been widely applied for aerobic process, however, the response of respirogram to anoxic denitrification is still unclear. To reveal such response may help to design a new method for the evaluation of the performance of denitrification. The size distribution of flocs measured at different denitrification moments demonstrated a clear expansion of flocs triggered by denitrification, during which higher specific endogenous and quasi-endogenous respiration rates (SOUR and SOUR) were also observed. Furthermore, SOUR increases exponentially with the specific denitrification rate (SDNR), suggesting that there should be a maximum SDNR in conventional activated sludge systems. Based on these findings, an index R, defined as the ratio of quasi-endogenous (OUR) to maximum respiration rate (OUR), is proposed to estimate the denitrification capacity that higher R indicates higher denitrification potential, which can be readily obtained without complex measurement or analysis, and it offers a novel and promising respirogram-based approach for denitrification estimation and control by taking measures to extend anoxic time to maintain its value at a high level within a certain range.
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http://dx.doi.org/10.1016/j.jes.2021.09.004DOI Listing
November 2022

Development and characterization of sodium alginate/tea tree essential oil nanoemulsion active film containing TiO nanoparticles for banana packaging.

Int J Biol Macromol 2022 Jul 27;213:145-154. Epub 2022 May 27.

International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China; Department of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo - Ourense Campus, E-32004 Ourense, Spain.

To improve the postharvest quality and reduce the anthracnose of bananas, the sodium alginate (SA)/tea tree essential oil nanoemulsion (TEON) based bilayer films incorporated with different contents of TiO nanoparticles were fabricated in this work. The developed TEON presented satisfactory DPPH scavenging activity (65.5 ± 1.8%) and antifungal capability (90.5 ± 5.8%). The microscopy images of the cross-section of the films indicated that the bilayer films with well-cross-linked were developed successfully. Notably, the addition of a certain content of TiO to SA greatly improved the UV blocking and water vapor and oxygen barrier properties of the developed film. However, the barrier property of the film was decreased when the TiO content further increased to 4.0 mg/100 mg of SA. The retention of TEO under visible and UV light in the bilayer film was respectively prolonged to 24 days and 32 h when the addition of TiO content was 2.0 mg/100 mg of SA. Finally, the postharvest quality and anthracnose of banana fruits were significantly improved by SA-TiO + SA-TEON film-forming solution treatments. Results from the present work might open up new insights into the approaches of postharvest quality improvement of bananas.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.05.164DOI Listing
July 2022

High-Performance Waveguide-Integrated Ge/Si Avalanche Photodetector with Lateral Multiplication Region.

Micromachines (Basel) 2022 Apr 19;13(5). Epub 2022 Apr 19.

Institute of Microelectronics, Chinese Academy of Sciences, Beijing 100029, China.

High-performance waveguide-integrated Ge/Si APDs in separate absorption, charge, and multiplication (SACM) schemes have been exploited to facilitate energy-efficient optical communication and interconnects. However, the charge layer design is complex and time-consuming. A waveguide-integrated Ge/Si avalanche photodetector (APD) is proposed in a separate absorption and multiplication (SAM) configuration. The device can work at low voltage and high speed with a lateral multiplication region without complexity of the charge layer. The proposed device is implemented by the complementary metal-oxide-semiconductor (CMOS) process in the 8-inch Si photonics platform. The device has a low breakdown voltage of 12 V and shows high responsivity of 15.1 A/W at 1550 nm wavelength under optical power of -22.49 dBm, corresponding to a multiplication gain of 18.1. Moreover, an opto-electrical bandwidth of 20.7 GHz is measured at 10.6 V. The high-speed performance at low voltage shows a great potential to implement high-energy-efficient Si optical communications and interconnections.
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http://dx.doi.org/10.3390/mi13050649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145896PMC
April 2022

Comprehensive Metabolomic Comparison of Five Cereal Vinegars Using Non-Targeted and Chemical Isotope Labeling LC-MS Analysis.

Metabolites 2022 May 10;12(5). Epub 2022 May 10.

Bioinformation and DDBJ Center, National Institute of Genetics, Mishima 411-8540, Japan.

Vinegar is used as an acidic condiment and preservative worldwide. In Asia, various black vinegars are made from different combinations of grains, such as Sichuan bran vinegar (SBV), Shanxi aged vinegar (SAV), Zhenjiang aromatic vinegar (ZAV), and Fujian Monascus vinegar (FMV) in China and Ehime black vinegar in Japan (JBV). Understanding the chemical compositions of different vinegars can provide information about nutritional values and the quality of the taste. This study investigated the vinegar metabolome using a combination of GC-MS, conventional LC-MS, and chemical isotope labeling LC-MS. Different types of vinegar contained different metabolites and concentrations. Amino acids and organic acids were found to be the main components. Tetrahydroharman-3-carboxylic acid and harmalan were identified first in vinegar. Various diketopiperazines and linear dipeptides contributing to different taste effects were also detected first in vinegar. Dipeptides, 3-phenyllactic acid, and tyrosine were found to be potential metabolic markers for differentiating vinegars. The differently expressed pathway between Chinese and Japanese vinegar was tryptophan metabolism, while the main difference within Chinese vinegars was aminoacyl-tRNA biosynthesis metabolism. These results not only give insights into the metabolites in famous types of cereal vinegar but also provide valuable knowledge for making vinegar with desirable health characteristics.
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http://dx.doi.org/10.3390/metabo12050427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144210PMC
May 2022
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