Publications by authors named "Zhigang Li"

714 Publications

Simultaneous screening and analysis of 155 veterinary drugs in livestock foods using ultra-high performance liquid chromatography tandem quadrupole linear-ion-trap mass spectrometry.

Food Chem 2022 Nov 20;393:133260. Epub 2022 May 20.

AB SCIEX, Analytical Instrument Trading Co, Beijing 100015, China.

Veterinary drugs are widely used to improve the health and growth of livestock. The supervision of these residues is necessary to ensure food safety. A high-throughput method based on Oasis PRiME HLB with solid phase extraction for simultaneous qualitative and quantitative analysis of 155 veterinary drugs in livestock foods was developed by the ultra-high performance liquid chromatography tandem quadrupole linear-ion-trap mass spectrometry (UHPLC-QTRAP-MS). The limits of detection and quantification ranged from 0.5 μg/kg to 5 μg/kg and 2 μg/kg to 20 μg/kg, respectively. For over 85% of the analytes, the recoveries were between 60% and 120%. The positive simulated samples perfectly matched with a purity fit value over 70% from the self-built library. The screening results of UHPLC-QTRAP-MS were almost consistent with UHPLC tandem quadrupole-exactive orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap/MS). The evaluated UHPLC-QTRAP-MS method was powerful and reliable for the screening and quantification of veterinary drugs in real samples.
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http://dx.doi.org/10.1016/j.foodchem.2022.133260DOI Listing
November 2022

MarZIC: A Marginal Mediation Model for Zero-Inflated Compositional Mediators with Applications to Microbiome Data.

Genes (Basel) 2022 Jun 11;13(6). Epub 2022 Jun 11.

Department of Biostatistics, University of Florida, Gainesville, FL 32611, USA.

Background: The human microbiome can contribute to pathogeneses of many complex diseases by mediating disease-leading causal pathways. However, standard mediation analysis methods are not adequate to analyze the microbiome as a mediator due to the excessive number of zero-valued sequencing reads in the data and that the relative abundances have to sum to one. The two main challenges raised by the zero-inflated data structure are: (a) disentangling the mediation effect induced by the point mass at zero; and (b) identifying the observed zero-valued data points that are not zero (i.e., false zeros).

Methods: We develop a novel marginal mediation analysis method under the potential-outcomes framework to address the issues. We also show that the marginal model can account for the compositional structure of microbiome data.

Results: The mediation effect can be decomposed into two components that are inherent to the two-part nature of zero-inflated distributions. With probabilistic models to account for observing zeros, we also address the challenge with false zeros. A comprehensive simulation study and the application in a real microbiome study showcase our approach in comparison with existing approaches.

Conclusions: When analyzing the zero-inflated microbiome composition as the mediators, MarZIC approach has better performance than standard causal mediation analysis approaches and existing competing approach.
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http://dx.doi.org/10.3390/genes13061049DOI Listing
June 2022

Cadherin-dependent adhesion modulated 3D cell-assembly.

J Mater Chem B 2022 Jun 22. Epub 2022 Jun 22.

Hefei National Research Center for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, China.

The emergence of synthetic biology has opened new avenues in constructing cell-assembly biosystems with specific gene expression and function. The phenomena of cell spreading and detachment during tissue development and cancer metastasis are caused by surface tension, which in turn results from differences in cell-cell adhesion mediated by the dimerization of cadherin expressed on the cell surface. In this study, E- and P-cadherin plasmids were first constructed based on the differential adhesion hypothesis, then they were electroporated into K562 cells and HEK293T cells, respectively, to explore the process of cell migration and assembly regulated by cadherins. Using this approach, some special 3D cell functional components with a phase separation structure were fabricated successfully. Our work will be of potential application in the construction of self-assembling synthetic tissues and organoids.
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http://dx.doi.org/10.1039/d2tb01006bDOI Listing
June 2022

Effect of Solvent Residue in the Thin-Film Fabrication on Perovskite Solar Cell Performance.

ACS Appl Mater Interfaces 2022 Jun 14. Epub 2022 Jun 14.

Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education; School of Materials Science and Engineering; Shaanxi Key Laboratory for Advanced Energy Devices; Shaanxi Engineering Lab for Advanced Energy Technology; Institute for Advanced Energy Materials, Shaanxi Normal University, Xi'an 710119, Shaanxi, China.

Organic-inorganic Pb-based halide perovskite photoelectrical materials, especially perovskite solar cells (PSCs), have attracted attention due to the significant efforts in improving the power conversion efficiency (PCE) to above 25%. However, the stability issue of the PSCs restricts their further development for commercialization. Strategies are designed to keep moisture and oxygen out of the perovskite films, such as additive, surface passivation, and solvent engineering; however, usually, the corrosion of active films by the residual solvent is mostly ignored. Solvent residue is the paramount factor influencing the stability of the perovskite film prepared by the solution method, and most solvents can be easily absorbed and accelerate the perovskite film decomposition. Here, we studied the residual solvent effect on two kinds of perovskite films obtained by different annealing processes: hot air annealing and hot bench annealing. Several detection techniques were used to study the performance of two different annealing methods, including time-of-flight secondary ion mass spectrometry (ToF-SIMS), thermogravimetric analysis (TGA), and field-emission scanning electron microscopy (FESEM). The perovskite film obtained by hot air annealing shows less residual solvent and better device performance than the hot bench annealing method. This method is expected to provide insight into reducing solvent residue to improve the stability of the PSCs, especially for future commercialization.
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http://dx.doi.org/10.1021/acsami.2c02525DOI Listing
June 2022

Effects of pre-operative enteral immunonutrition for esophageal cancer patients treated with neoadjuvant chemoradiotherapy: protocol for a multicenter randomized controlled trial (point trial, pre-operative immunonutrition therapy).

BMC Cancer 2022 Jun 13;22(1):650. Epub 2022 Jun 13.

Department of Thoracic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin Er Road, Shanghai, 200025, China.

Background: Neoadjuvant chemoradiation followed by esophagectomy has been established as the first-line treatment for locally advanced esophageal cancer. Postoperative enteral nutrition has been widely used to improve perioperative outcomes. However, whether to implement preoperative nutritional intervention during neoadjuvant therapy is yet to be verified by prospective studies.

Methods: POINT trial is a multicenter, open-labeled, randomized controlled trial. A total of 244 patients with surgically resectable esophageal cancer are randomly assigned to nutritional therapy group (arm A) or control group (arm B) with a 2:1 ratio. Both groups receive neoadjuvant chemotherapy with concurrent radiotherapy based on the CROSS regimen followed by minimally invasive esophagectomy. The primary endpoint is the rate of nutrition and immune-related complications after surgery. Secondary endpoints include completion rate of neoadjuvant chemoradiation and related adverse events, rate of pathological complete response, perioperative outcomes, nutritional status, overall survival, progression-free survival and quality of life.

Discussion: This trial aims to verify whether immunonutrition during neoadjuvant chemoradiation can reduce the rate of complications and improve perioperative outcomes. Frequent communication and monitoring are essential for a multicenter investigator-initiated trial.

Trial Registration: ClinicalTrials.gov: NCT04513418. The trial was prospectively registered on 14 August 2020, https://www.

Clinicaltrials: gov/ct2/show/NCT04513418 .
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http://dx.doi.org/10.1186/s12885-022-09721-yDOI Listing
June 2022

LncRNA JPX Promotes Esophageal Squamous Cell Carcinoma Progression by Targeting miR-516b-5p/VEGFA Axis.

Cancers (Basel) 2022 May 31;14(11). Epub 2022 May 31.

Department of Thoracic Surgery, Section of Esophageal Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China.

Long non-coding RNAs (lncRNAs) are reported act as important regulators in various types of cancer. LncRNA JPX was identified as an oncogenic regulator in lung cancer. However, the function of JPX in the progression of esophageal squamous cell carcinoma (ESCC) remains unclear. In the present study, we found JPX was highly expressed in esophageal tissue from ESCC patients. Functional assays demonstrated that JPX promoted ESCC cell proliferation, migration, and invasion in vitro, and accelerated tumor growth in vivo. Mechanistically, the results showed that JPX functioned as a sponge of miR-516b-5p, which targeted vascular endothelial growth factor A (VEGFA) in ESCC cells. Interactions between miR-516b-5p and JPX or VEGFA were confirmed by luciferase reporter assays. Inhibition of JPX significantly attenuated the cell growth and mobility ability of ESCC cells in vitro. In addition, overexpression of miR-516b-5p abrogated JPX-enhanced proliferation, migration, invasion, and angiogenesis of ESCC cells. Our study demonstrated that JPX played an important role in promoting ESCC progression via the miR-516b-5p/VEGFA pathway, which might serve as a promising novel diagnostic biomarker and therapeutic target for ESCC in clinic.
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http://dx.doi.org/10.3390/cancers14112713DOI Listing
May 2022

Six Types of Tea Reduce Acute Alcoholism in Mice by Enhancing Ethanol Metabolism, Suppressing Oxidative Stress and Inflammation.

Front Nutr 2022 23;9:848918. Epub 2022 May 23.

College of Tea Science, Yunnan Agricultural University, Kunming, China.

Acute alcoholic intoxication (AAI) is a pathological process of multiple system damage caused by a large amount of alcohol, especially in the liver. Although tea extracts alleviate AAI and alcohol-induced liver damage, the mechanisms underlying the protective actions of different types of Chinese tea are unclear. In this study, the AAI mice model was used to explore the functions and mechanisms of six types of tea extract (WEATs) in alleviating AAI. The losing righting reflexes of mice were evaluated to assess the effects of the WEATs on AAI. The levels of the ethanol metabolism enzymes (ADH, ALDH2, CYP2E1), the oxidative stress-related indicators (NRF-2, HO-1, SOD, GSH, CAT, and TG) and the inflammatory factors (TNF-α, iNOS, IL-6, and IL-10) were determined. Black tea and dark tea significantly shortened the sleep time (duration of the loss of righting reflex) and had a good sobering effect. Green tea and oolong tea had the dual effect of prolonging tolerance time (time of losing righting reflex) and shortening sleep time. While white tea had the most significant effect on prolonging tolerance time but with no obvious sobering effect. Black tea, dark tea, and oolong tea significantly up-regulated ADH and ALDH2, and down-regulated CYP2E1. Green tea and white tea significantly increased the levels of Nrf2, GSH, and CAT. Black tea, dark tea and oolong tea markedly increased the levels of HO-1, IL-10, and inhibited TG. Therefore, it is possible that black tea, dark tea and oolong tea reduced AAI by increasing ethanol metabolism, suppressing oxidative stress and inflammation. While green tea was mainly by regulating oxidative stress. White tea may prolong the tolerance time by increasing ethanol metabolism and reducing oxidative stress. Different types of tea have specific chemical compositions and can alleviate AAI. In conclusion, despite variations in the composition and mechanism of action, tea is a potent natural product to alleviate a hangover and protect the liver.
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http://dx.doi.org/10.3389/fnut.2022.848918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169692PMC
May 2022

A Cooperative Lightweight Translation Algorithm Combined with Sparse-ReLU.

Comput Intell Neurosci 2022 28;2022:4398839. Epub 2022 May 28.

Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China.

In the field of natural language processing (NLP), machine translation algorithm based on Transformer is challenging to deploy on hardware due to a large number of parameters and low parametric sparsity of the network weights. Meanwhile, the accuracy of lightweight machine translation networks also needs to be improved. To solve this problem, we first design a new activation function, Sparse-ReLU, to improve the parametric sparsity of weights and feature maps, which facilitates hardware deployment. Secondly, we design a novel cooperative processing scheme with CNN and Transformer and use Sparse-ReLU to improve the accuracy of the translation algorithm. Experimental results show that our method, which combines Transformer and CNN with the Sparse-ReLU, achieves a 2.32% BLEU improvement in prediction accuracy and reduces the number of parameters of the model by 23%, and the sparsity of the inference model increases by more than 50%.
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http://dx.doi.org/10.1155/2022/4398839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167065PMC
June 2022

Safety and efficacy of neoadjuvant treatment with immune checkpoint inhibitors in esophageal cancer: real-world multicenter retrospective study in China.

Dis Esophagus 2022 Jun 2. Epub 2022 Jun 2.

Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, P. R. China.

Immune checkpoint inhibitors (ICIs) have shown a powerful benefit in the neoadjuvant therapy for esophageal cancer, but evidence for its safety and efficacy is limited and may not reflect real-world practice. We retrospectively reviewed the database of treatment-naive patients from 15 esophageal cancer centers in China who received ICIs as neoadjuvant treatment for locally advanced esophageal cancer from May 2019 to December 2020. The primary endpoints were rate and severity of treatment-related adverse events (TRAEs) and immune-related adverse events (irAEs). Secondary endpoints included pathologically complete response (pCR) rate, R0 resection rate, mortality and morbidity. Among the 370 patients, 311 (84.1%) were male with a median age of 63 (range: 30-81) years and stage III or IVa disease accounted for 84.1% of these patients. A total of 299 (80.8%) patients were treated with ICIs and chemotherapy. TRAEs were observed in 199 (53.8%) patients with low severity (grade 1-2, 39.2%; grade 3-4, 13.2%; grade 5, 1.4%), and irAEs occurred in 24.3% of patients and were mostly of grade 1-2 severity (21.1%). A total of 341 (92.2%) patients had received surgery and R0 resection was achieved in 333 (97.7%) patients. The local pCR rate in primary tumor was 34.6%, including 25.8% of ypT0N0 and 8.8% of ypT0N+. The rate of postoperative complications was 41.4% and grade 3 or higher complications occurred in 35 (10.3%) patients. No death was observed within 30 days after surgery, and three patients (0.9%) died within 90 days postoperatively. This study shows acceptable toxicity of neoadjuvant immunotherapy for locally advanced esophageal cancer in real-world data. Long-term survival results are pending for further investigations.
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http://dx.doi.org/10.1093/dote/doac031DOI Listing
June 2022

Value of contrast-enhanced mammography combined with the Kaiser score for clinical decision-making regarding tomosynthesis BI-RADS 4A lesions.

Eur Radiol 2022 May 31. Epub 2022 May 31.

Department of Computed Tomography and Magnetic Resonance, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China.

Objectives: To investigate the diagnostic performance of contrast-enhanced mammography (CEM) combined with the Kaiser score (KS) in digital breast tomosynthesis (DBT) BI-RADS 4A lesions to potentially reduce unnecessary breast biopsies.

Methods: This retrospective study evaluated 106 patients with 109 DBT BI-RADS 4A lesions from June 2019 to June 2021. For the absence of enhancement on CEM, the lesions were downgraded to BI-RADS 3. For lesions with enhancement, the readers were asked to classify all enhancing lesions referring to the KS for breast MRI. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic performance. Two readers rated all cases and interreader agreement was assessed by Cohen's kappa coefficients.

Results: There were ninety-five benign lesions and 14 malignant lesions. CEM combined with KS's accuracy, represented by the area under the curve (AUC), ranged between 0.880 and 0.906. The use of the KS improved the performance, with a significant difference relative to a single BI-RADS reading or US (p < 0.001). CEM with KS had higher specificity than CEM with BI-RADS or US (p < 0.001), without difference in sensitivity (p > 0.05). CEM combined with KS could have potentially obviated 72 (75.8%) to 78 (82.1%) unnecessary benign biopsies in 95 benign lesions previously DBT classified as BI-RADS 4A. The interreader agreement was substantial (kappa: 0.727) for KS.

Conclusions: CEM combined with KS may be used in DBT BI-RADS 4A lesions to substantially reduce unnecessary benign biopsies.

Key Points: • CEM combined with the Kaiser scoring system shows high diagnostic performance for DBT BI-RADS 4A lesions. • The application of CEM combined with the Kaiser scoring system may avoid 75.8% to 82.1% of unnecessary benign breast biopsies. • CEM combined with the KS aids clinical decision-making in DBT BI-RADS 4A lesions.
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http://dx.doi.org/10.1007/s00330-022-08810-7DOI Listing
May 2022

Chemical characterization, source apportionment, and health risk assessment of PM in a typical industrial region in North China.

Environ Sci Pollut Res Int 2022 May 23. Epub 2022 May 23.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.

To clarify the chemical characteristics, source contributions, and health risks of pollution events associated with high PM in typical industrial areas of North China, manual sampling and analysis of PM were conducted in the spring, summer, autumn, and winter of 2019 in Pingyin County, Jinan City, Shandong Province. The results showed that the total concentration of 29 components in PM was 53.4 ± 43.9 μg·m, including OC/EC, water-soluble ions, inorganic elements, and metal elements. The largest contribution was from the NO ion, at 14.6 ± 14.2 μg·m, followed by organic carbon (OC), SO, and NH, with concentrations of 9.3 ± 5.5, 9.1 ± 6.4, and 8.1 ± 6.8 μg·m, respectively. The concentrations of OC, NO, and SO were highest in winter and lowest in summer, whereas the NH concentration was highest in winter and lowest in spring. Typical heavy metals had higher concentrations in autumn and winter, and lower concentrations in spring and summer. The annual average sulfur oxidation rate (SOR) and nitrogen oxidation rate (NOR) were 0.30 ± 0.14 and 0.21 ± 0.12, respectively, with the highest SO emission and conversion rates in winter, resulting in the SO concentration being highest in winter. The average concentration of secondary organic carbon in 2019 was 2.8 ± 1.9 μg·m, and it comprised approximately 30% of total OC. The concentrations of 18 elements including Na, Mg, and Al were between 2.3 ± 1.6 and 888.1 ± 415.2 ng·m, with Ni having the lowest concentration and K the highest. The health risk assessment for typical heavy metals showed that Pb poses a potential carcinogenic risk for adults, whereas As may pose a carcinogenic risk for adults, children, and adolescents. The non-carcinogenic risk coefficients for all heavy metals were lower than 1.0, indicating that the non-carcinogenic risk was negligible. Positive matrix factorization analysis indicated that coal-burning emissions contributed the largest fraction of PM, accounting for 35.9% of the total. The contribution of automotive emissions is similar to that of coal, at 32.1%. The third-largest contributor was industrial sources, which accounted for 17.2%. The contributions of dust and other emissions sources to PM were 8.4% and 6.4%, respectively. This study provides reference data for policymakers to improve the air quality in the NCP.
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http://dx.doi.org/10.1007/s11356-022-19843-2DOI Listing
May 2022

Metal nanoparticles increased the lag period and shaped the microbial community in slurry-electrode microbial electrosynthesis.

Sci Total Environ 2022 Sep 16;838(Pt 1):156008. Epub 2022 May 16.

Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Concerns about energy crisis and CO emission have motivated the development of microbial electrosynthesis (MES); recent studies have showed the potential of novel slurry-electrode MES. In this study, the effect of nonprecious metal nanoparticles (NPs) on the performance of slurry-electrode MES was systematically evaluated in terms of chemical production, physicochemical properties, electrochemical characterization, and microbial community. Ni and Cu NPs increased the lag period from 6 to 15 days for acetate production, while Mo NPs showed no apparent effect. However, these metal NPs slightly affected the final total acetate production (ca. 10 g L), Faradic efficiency (ca. 50%), net water flux across the anion exchange membrane (ca. 6 mL d), or electrochemical characterization of catholyte. BRH-c20a was enriched as the dominated microbe (>48%), and its relative abundance was largely affected by the addition of metal NPs. This study demonstrates that metal NPs affect the performance of biocathodes, mainly by shaping the microbial community.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156008DOI Listing
September 2022

Scutellarin Improves Type 2 Diabetic Cardiomyopathy by Regulating Cardiomyocyte Autophagy and Apoptosis.

Dis Markers 2022 6;2022:3058354. Epub 2022 May 6.

Department of Biochemistry and Molecular Biology, School of Basic Medicine, Kunming Medical University, Kunming, Yunnan 650500, China.

Diabetes cardiomyopathy has metabolic disorder and abnormality of cardiomyocytes, which is closely related to autophagy or apoptosis of cardiomyocytes. Scutellarin (SCU) is an important monomer extracted from (vant.) Hand.-Mazz. This study was conducted to investigate the function of SCU on apoptosis and autophagy of myocardial cells. We established a model of type 2 diabetic cardiomyopathy by high-fat and high-sugar diet. The results indicated that SCU downregulated blood glucose, total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL) levels and upregulated high-density lipoprotein (HDL) level. In addition, SCU downregulated lactic dehydrogenase 1 (LDH1) and creatine kinase (CK) levels. Meanwhile, SCU improved the myocardium morphology and reduced myocardial apoptosis. Furthermore, SCU promoted the mRNA and protein expression of autophagy-related factors (Beclin-1 and LC3-II) and inhibited the mRNA and protein expression of apoptosis-related factors (caspase-3, caspase-8, caspase-9, caspase-12, Bax, and Cyt-C). In conclusion, SCU can promote autophagy signal pathway by upregulating the autophagy-related factors and inhibit the apoptotic signal pathway by downregulating apoptosis-related factors, thereby relieving type 2 diabetic cardiomyopathy (T2DC).
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http://dx.doi.org/10.1155/2022/3058354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106511PMC
May 2022

Patterns of recurrence after robot-assisted minimally invasive esophagectomy in esophageal squamous cell carcinoma.

Semin Thorac Cardiovasc Surg 2022 May 8. Epub 2022 May 8.

Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Objective: Robot-assisted minimally invasive esophagectomy (RAMIE) has been proven to be a feasible surgical approach for esophageal squamous cell carcinoma (ESCC). This study aimed to investigate the recurrence pattern and potential risk factors after RAMIE.

Methods: Consecutive patients with ESCC who received RAMIE with McKeown technique at a single Esophageal Cancer Institute from November 2015 to September 2018 were retrospectively reviewed. Patients with available data, radical resection (R0) and a minimum 2-year follow-up period were eligible for the recurrence analysis. Risk factors of recurrence were examined by logistic regression analysis.

Results: R0 resection was achieved in 95.1% of patients (310/326). Of the 298 eligible patients with a median follow-up period of 30.6 months, recurrence was recognized in 95 patients (31.9%), with 4 (1.3%) local-only, 40 (13.4%) regional-only, 44 (14.8%) hematogenous-only and 7 (2.3%) combined recurrences. Cervical lymph nodes and lung were the most frequently sites of regional and hematogenous recurrence, respectively. The median disease-free interval until recurrence was 12.1 (range 1.7-37.6) months and 83.2% of relapse happened within 2 years after surgery. Multivariable analysis indicated that tumor in the upper esophagus, larger tumor length and positive lymph nodes as independent risk factors for recurrence.

Conclusions: Hematogenous recurrence is the prevailing pattern after RAMIE for ESCC. For patients with advanced disease, neoadjuvant therapy is a key factor in reducing recurrence rather than surgical approaches.
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http://dx.doi.org/10.1053/j.semtcvs.2022.04.008DOI Listing
May 2022

Three-arm phase II trial comparing camrelizumab plus chemotherapy versus camrelizumab plus chemoradiation versus chemoradiation as preoperative treatment for locally advanced esophageal squamous cell carcinoma (NICE-2 Study).

BMC Cancer 2022 May 6;22(1):506. Epub 2022 May 6.

Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, 241 West Huaihai Road, Shanghai, 200030, China.

Background: Preoperative chemoradiotherapy (CRT) with CROSS regimen has been the recommended treatment for locally advanced esophageal squamous cell carcinoma (ESCC). The addition of programmed cell death protein 1 (PD-1) inhibitor to preoperative CRT may further improve oncologic results. Preoperative camrelizumab plus chemotherapy has been demonstrated as a promising treatment modality based on results of the phase II NICE study (ChiCTR1900026240).

Methods: The NICE-2 study is designed as a three-arm, multicenter, prospective, randomized, phase II clinical trial, comparing camrelizumab plus chemotherapy (IO-CT) and camrelizumab plus CRT (IO-CRT) versus CRT as preoperative treatment for locally advanced ESCC. A total of 204 patients will be recruited from 8 Chinese institutions within 1.5 years. The primary endpoint is pathological complete response (pCR) rate and secondary endpoints include event-free survival (EFS), R0 resection rate, and adverse events.

Discussion: This is the first prospective randomized controlled trial to explore commonly used neoadjuvant treatments in clinical practice, which will provide high-level evidence of neoadjuvant treatment for patients with locally advanced ESCC. The purpose of this study is to establish the optimal modality of IO-CT, IO-CRT and CRT as preoperative treatment for locally advanced ESCC. The Institution Review Committee approved this study protocol in August 2021 and patient enrollment was started in September 2021.

Trial Registration: ClinicalTrial.gov: NCT05043688 (August 29, 2021). The trial was prospectively registered.
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http://dx.doi.org/10.1186/s12885-022-09573-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074348PMC
May 2022

F-FDG PET/CT for Identifying the Potential Primary Diseases and Predicting Prognosis of Secondary Hemophagocytic Lymphohistiocytosis in Children.

Contrast Media Mol Imaging 2022 16;2022:4849081. Epub 2022 Apr 16.

Hematology Center, Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Disease in Children, Ministry of Education, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.

Hemophagocytic lymphohistiocytosis (HLH) is a rare, potentially fatal illness, which can be divided into primary HLH (pHLH) and secondary HLH (sHLH). pHLH can be driven by genetic defections. Moreover, the sHLH is usually be triggered by malignancy or non-malignancy diseases. Sixty-two newly diagnosed sHLH patients with known etiology and those who underwent F-FDG PET/CT examination from July 2018 to December 2020 were retrospectively analyzed. They were divided into malignancy-associated HLH (M-HLH,  = 13) and non-malignancy-associated HLH (NM-HLH,  = 49). The metabolic parameters of the liver (Li), spleen (Sp), bone marrow (BM), lymph nodes (LN), and their ratios to the liver background (LiBG) and mediastinum (M) were compared between two groups. These metabolic parameters were evaluated for correlation with laboratory parameters and prognostic parameters. We found that the SUV-LN/Sp/Li and SUV-Sp in M-HLH were significantly higher than those in NM-HLH (=0.031, 0.035, 0.016,  and 0.032). The malignant disease should be considered when SUVmax-LN was higher than 4.41 (sensitivity 61.5%, specificity 81.6%). Hypermetabolic lesions in extranodal organs were more likely to occur in M-HLH than in NM-HLH (=0.011). IFN- was positively correlated with SUVmax-BM/Li/Sp and SUVmean-BM/Li/Sp ( < 0.05). Ferritin, sCD25, IL-6, and IL-10 were positively correlated with SUVmax-Sp and SUVmean-Sp ( < 0.05). In Epstein-Barr virus-associated HLH (EBV-HLH), the SUV parameters of bone marrow were significantly correlated with a poor 2-week treatment response, overall survival, and event-free survival ( < 0.05). We conclude that some F-FDG PET/CT metabolic parameters can help identify the etiology of sHLH in children and provide directions for further inspection. The malignant disease should be considered when the SUVmax-LN is higher than 4.41 and hypermetabolic lesions occur in extranodal organs. In EBV-HLH, a higher SUV of bone marrow is associated with a poorer prognosis.
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http://dx.doi.org/10.1155/2022/4849081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034951PMC
April 2022

Rapid SERS identification of methicillin-susceptible and methicillin-resistant aptamer recognition and deep learning.

RSC Adv 2021 Oct 25;11(55):34425-34431. Epub 2021 Oct 25.

Hefei Institute of Physical Science, Chinese Academy of Sciences Hefei 230036 P. R China

Here, we report a label-free surface-enhanced Raman scattering (SERS) method for the rapid and accurate identification of methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) based on aptamer-guided AgNP enhancement and convolutional neural network (CNN) classification. Sixty clinical isolates of (), comprising 30 strains of MSSA and 30 strains of MRSA were used to build the CNN classification model. The developed method exhibited 100% identification accuracy for MSSA and MRSA, and is thus a promising tool for the rapid detection of drug-sensitive and drug-resistant bacterial strains.
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http://dx.doi.org/10.1039/d1ra05778bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042729PMC
October 2021

How fluorescent labels affect the kinetics of the toehold-mediated DNA strand displacement reaction.

Chem Commun (Camb) 2022 May 12;58(39):5849-5852. Epub 2022 May 12.

Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.

End modification of the toehold domain or near to it using fluorophore dyes or quenchers can significantly modulate the kinetics of the toehold-mediated strand displacement reaction (TMSDR) by almost two orders of magnitude. The labels at the end of the signal strand impede the TMSDR, while those at the end of the toehold domain of the substrate strand accelerate the TMSDR kinetics.
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http://dx.doi.org/10.1039/d2cc01072kDOI Listing
May 2022

Single-Cell RNA Sequencing Reveals Heterogeneity of Myf5-Derived Cells and Altered Myogenic Fate in the Absence of SRSF2.

Adv Sci (Weinh) 2022 06 23;9(18):e2105775. Epub 2022 Apr 23.

CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, P. R. China.

Splicing factor SRSF2 acts as a critical regulator for cell survival, however, it remains unknown whether SRSF2 is involved in myoblast proliferation and myogenesis. Here, knockdown of SRSF2 in myoblasts causes high rates of apoptosis and defective differentiation. Combined conditional knockout and lineage tracing approaches show that Myf5-cre mice lacking SRSF2 die immediately at birth and exhibit a complete absence of mature myofibers. Mutant Myf5-derived cells (tdtomato-positive cells) are randomly scattered in the myogenic and non-myogenic regions, indicating loss of the community effect required for skeletal muscle differentiation. Single-cell RNA-sequencing reveals high heterogeneity of myf5-derived cells and non-myogenic cells are significantly increased at the expense of skeletal muscle cells in the absence of SRSF2, reflecting altered cell fate. SRSF2 is demonstrated to regulate the entry of Myf5 cells into the myogenic program and ensures their survival by preventing precocious differentiation and apoptosis. In summary, SRSF2 functions as an essential regulator for Myf5-derived cells to respond correctly to positional cues and to adopt their myogenic fate.
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http://dx.doi.org/10.1002/advs.202105775DOI Listing
June 2022

Clinical significance of cerebrospinal fluid soluble CD25 in pediatric hemophagocytic lymphohistiocytosis with central nervous system involvement.

Pediatr Blood Cancer 2022 Aug 20;69(8):e29712. Epub 2022 Apr 20.

Hematology Center, Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Disease in Children, Ministry of Education, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

Objective: To analyze the clinical significance of soluble CD25 (sCD25) levels in cerebrospinal fluid (CSF) in pediatric hemophagocytic lymphohistiocytosis (HLH) with central nervous system (CNS) involvement.

Methods: All patients diagnosed with HLH admitted to Beijing Children's Hospital, Capital Medical University between January 1, 2017 and October 31, 2021 who received a measurement of their HLH-related parameters and CSF sCD25 levels at admission were enrolled in this study.

Results: CSF sCD25 levels in patients with primary HLH were higher than those in patients with Epstein-Barr virus infection-associated HLH, and the median level was 444 pg/ml. The difference in CSF sCD25 levels between the non-CNS group and the CNS group was statistically significant (591 [259-33,643] pg/ml vs. 123 (36-437) pg/ml, p < .001). The best cutoff value of CSF sCD25 was 273.5 pg/ml (AUC = 0.987, 95% CI: 0.972-1.000), with sensitivity, specificity, positive predictive values, and negative predictive values of 96.4%, 92.8%, 81.8%, and 98.7%, respectively. CSF sCD25 in the severe CNS involvement group was significantly higher than that in the nonsevere CNS involvement group (p = .014). The 3-year overall survival (OS) of patients with high CSF sCD25 levels was lower than that of patients with low CSF sCD25 levels(71.6% ± 8.1% vs. 93.3% ± 2.9%, hazard ratio [HR] = 3.637, p = .003).

Conclusion: Increased CSF sCD25 levels in active disease can predict CNS-HLH. Primary HLH has a higher CSF sCD25 level than Epstein-Barr virus infection-associated HLH. Patients who are diagnosed with CNS-HLH with CSF sCD25 levels higher than 273.5 pg/ml are more likely to develop severe CNS involvement, suggesting a poor prognosis.
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http://dx.doi.org/10.1002/pbc.29712DOI Listing
August 2022

An Ego Depletion Perspective Linking Political Behavior to Interpersonal Deviance.

Front Psychol 2022 30;13:802636. Epub 2022 Mar 30.

School of Economics and Management, Dalian University of Technology, Dalian, China.

A political act is a typical self-serving behavior that works to promote or protect self-interest. However, limited research explores its relationship with daily downstream behavior. Based on the ego depletion theory, the present study attempts to clarify when and how daily political acts will be transformed into interpersonal deviance. We collected 760 cases nested in 152 full time workers in mainland China through the experience sampling method. a multilevel structural equation model and hierarchical linear model, we tested the mediated moderation model. The results showed that political acts correlated with interpersonal deviance on a daily basis. Moral self-efficacy buffers the relationship between political acts and interpersonal deviance, whereas chronic job strain amplifies the relationship. Furthermore, moral self-efficacy can mediate the moderating role of chronic job strain.
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http://dx.doi.org/10.3389/fpsyg.2022.802636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006816PMC
March 2022

Controllable perfect optical vortex generated by complex amplitude encoding.

Opt Lett 2022 Apr;47(8):2101-2104

We propose a new paradigm for generating the perfect optical vortex (POV) with a controlled structure and orbital angular momentum (OAM) distribution in the focal region of a tightly focused system. The superiority of the proposed technique is demonstrated with an experiment involving the dynamic manipulation of small particles. This technique for creating the POV could open new routes to optical manipulation based on OAM.
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http://dx.doi.org/10.1364/OL.433864DOI Listing
April 2022

Release of methane from nanochannels through displacement using CO.

RSC Adv 2021 Apr 26;11(25):15457-15466. Epub 2021 Apr 26.

Key Laboratory of Thermo-Fluid Science and Engineering of MOE, School of Energy and Power Engineering, Xi'an Jiaotong University Xi'an Shaanxi 710049 PR China

In this work, we investigate the release of methane in quartz nanochannels through the method of displacement using carbon dioxide. Molecular dynamics (MD) simulations and theoretical analysis are performed to obtain the release percentage of methane for nanochannels of various diameters. It is found that both the pressure of CO and the channel size affect the release percentage of methane, which increases with increasing pressure of CO and channel diameter. Without CO, the majority of methane molecules are adsorbed by the channel surface. When CO is injected into the channel, CO molecules replace many methane molecules due to the relatively strong molecular interactions between CO and the channel, which leads to the desorption of methane, reduces the energy barrier for the transport of methane, and consequently increases the release rate. Theoretical predictions using the kinetic energy of methane and the energy barrier inside the channel are also conducted, which are in good agreement with the MD simulations.
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http://dx.doi.org/10.1039/d1ra01795kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698835PMC
April 2021

Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of .

RSC Adv 2021 Apr 9;11(22):13297-13303. Epub 2021 Apr 9.

Hefei Institute of Physical Science, Chinese Academy of Sciences Hefei 230036 PR China

This paper reports a colorimetric-fluorescent dual-signal lateral flow assay (LFA) based on vancomycin (Van)-modified SiO-Au-QD tags for sensitive and quantitative detection of (). The combination of high-performance Van-tags and detection antibodies integrated into the LFA system produced assays with high sensitivity and specificity. The visualization limit of the colorimetric signal and the detection limit of the fluorescence signal of the proposed method for can reach 10 and 100 cells mL, respectively.
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http://dx.doi.org/10.1039/d1ra01085aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697553PMC
April 2021

Directional passive transport of nanodroplets on general axisymmetric surfaces.

Phys Chem Chem Phys 2022 Apr 20;24(16):9727-9734. Epub 2022 Apr 20.

Jiangsu Key Laboratory for Design & Manufacture of Micro/Nano Biomedical Instruments and School of Mechanical Engineering, Southeast University, Nanjing 210096, P. R. China.

Rapid removal of small-sized droplets passively using fixed structures is a key challenge for various applications including anti-icing, rapid cooling, and water harvesting. In this work, we investigate the directional motion of nanodroplets on axisymmetric surfaces with curvature gradient through molecular dynamics (MD) simulations. It is found that as the shape of the axisymmetric surface is changed from a dome to a trumpet, the droplet velocity is greatly enhanced, by a factor of ∼14. Such an increase is mainly caused by the increment in the driving force. The droplet velocity changes nonlinearly as the surface wettability is varied and assumes the maximum at the contact angle of ∼75°. We derive a formula for the driving force of nanodroplets on general axisymmetric surfaces by evaluating the pressure gradient inside the droplet induced by the curvature gradient. Molecular dynamics simulations are performed to directly measure the driving force and confirm that the theoretical formula works well. By illustrating the reduced initial velocity of droplets as a function of a dimensionless number, which represents the ratio of the driving force to the retentive force due to contact angle hysteresis, we show that the onset of droplet motion on axisymmetric surfaces occurs when the dimensionless number is above a critical value. The dimensionless number reveals the effects of surface geometry, surface wettability, and droplet size on the droplet motion.
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http://dx.doi.org/10.1039/d1cp05905jDOI Listing
April 2022

Prevalence of multiple sclerosis and treatment utilization in a large, highly diverse population.

Mult Scler Relat Disord 2022 May 30;61:103784. Epub 2022 Mar 30.

Department of Neurology, University of Florida, Gainesville, FL, USA.

Background: Despite advances in algorithms for identifying people with MS (PwMS) in large data sets, limited data exists on regional prevalence, or prevalence and care in minority populations.

Objectives: To report the 7-year (01/01/2012-12/31/2018) prevalence and demographics of MS and disease-modifying therapy (DMT) utilization in a large, diverse population.

Methods: This retrospective analysis used the OneFlorida Data Trust, which captures health data from >15 million Floridians across 10 constituent organizations. A validated algorithm identified subjects with MS. DMTs were identified using RxNorm concept unique identifiers and National Drug Codes. Results were stratified across age, sex, race-ethnicity, and location.

Results: Of 6,638,649 adults in the database, the algorithm identified 9681 PwMS. Overall prevalence per 100,000 was 145.83. MS prevalence was considerable in women of all races and ethnicities ranging from 138.86 to 253.76 per 100,000. 52.6% of PwMS had one or more DMT prescription. DMT prescription was more likely in Hispanic PwMS.

Conclusion: Prevalence analysis of the OneFlorida Data Trust revealed a substantial burden of disease in women of all races and ethnicities. Variation in treatment utilization among demographic subgroups underscores the need for additional studies to assess health care disparities in MS at the population level.
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http://dx.doi.org/10.1016/j.msard.2022.103784DOI Listing
May 2022

Aged green tea reduces high-fat diet-induced fat accumulation and inflammation via activating the AMP-activated protein kinase signaling pathway.

Food Nutr Res 2022 10;66. Epub 2022 Mar 10.

Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization, Guangzhou, China.

Background: Obesity is a global public health concern and increases the risk of metabolic syndrome and other diseases. The anti-obesity effects of various plant-derived bioactive compounds, such as tea extracts, are well-established. The mechanisms underlying the anti-obesity activity of Jinxuan green tea (JXGT) from different storage years are still unclear.

Objective: The aim of this study was to evaluate the effects of JXGTs from three different years on the high fat diet (HFD)-fed mouse model.

Design: The mice were divided into six groups, the control group received normal diet and the obese model group received HFD. We analyzed the effects of JXGTs from 2005, 2008, and 2016 on HFD-fed obese mice over a period of 7 weeks.

Results: The JXGTs reduced the body weight of the obese mice, and also alleviated fat accumulation and hepatic steatosis. Mechanistically, JXGTs increased the phosphorylation of AMP-activated protein kinase (p-AMPK)/AMP-activated protein kinase (AMPK) ratio, up-regulated carnitine acyl transferase 1A (CPT-1A), and down-regulated fatty acid synthase (FAS), Glycogen synthase kinase-3beta (GSK-3β), Peroxisome proliferator-activated receptor-gamma co-activator-1alpha (PGC-1α), Interleukin 6 (IL-6), and Tumour necrosis factor alpha (TNFα). Thus, JXGTs can alleviate HFD-induced obesity by inhibiting lipid biosynthesis and inflammation, thereby promoting fatty acid oxidation via the AMPK pathway.

Discussion: The anti-obesity effect of three aged JXGTs were similar. However, JXGT2016 exhibited a more potent activation of AMPK, and JXGT2005 and JXGT2008 exhibited a more potent inhibiting glycogen synthase and inflammation effect. Furthermore, the polyphenol (-)-epicatechin (EC) showed the strongest positive correlation with the anti-obesity effect of JXGT.

Conclusions: These findings demonstrate that JXGT treatment has a potential protection on HFD-induced obesity mice via activating the AMPK/CPT-1A and down-regulating FAS/GSK-3β/PGC-1α and IL-6/TNFα. Our study results also revealed that different storage time would not affect the anti-obesity and anti-inflammation effect of JXGT.

Graphical Abstract:
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http://dx.doi.org/10.29219/fnr.v66.7923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8941417PMC
March 2022

Familial hemophagocytic lymphohistiocytosis onset as central diabetes insipidus in a child.

Pediatr Blood Cancer 2022 Apr 4:e29684. Epub 2022 Apr 4.

Hematology Center, Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Disease in Children, Ministry of Education, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

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http://dx.doi.org/10.1002/pbc.29684DOI Listing
April 2022

Efficient production of medium chain fatty acids in microbial electrosynthesis with simultaneous bio-utilization of carbon dioxide and ethanol.

Bioresour Technol 2022 May 31;352:127101. Epub 2022 Mar 31.

Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Microbial electrosynthesis (MES) is a promising technology for chemicals production driven by renewable energy. However, how the medium chain fatty acids (MCFAs) production in MES is affected by the method of chain elongation is not clear, and no direct evidence is provided yet for a simultaneous bio-utilization of CO and ethanol. In this study, different methods of chain elongation in MES reactors were investigated. During in-situ chain elongation, a maximum caproate concentration of 11.9 ± 0.6 g L was achieved, while the C6 specificity (56.4% ± 0.5%) was much lower than that of ex-situ chain elongation (78.7% ± 1.5%). Carbon distribution and reduction degree balance indicated a simultaneous bio-utilization of CO and ethanol, and it was validated by the isotope tracer technique. MCFAs-forming microbes, acetogens, and electrochemically active microorganisms were enriched. This study provides fundamental insights relevant to the carbon and electron fluxes driven by electricity.
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http://dx.doi.org/10.1016/j.biortech.2022.127101DOI Listing
May 2022

Quantitative analysis of Raman spectra for glucose concentration in human blood using Gramian angular field and convolutional neural network.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jul 26;275:121189. Epub 2022 Mar 26.

College of Information Science and Engineering, Northeastern University, Shenyang, Liaoning Province 110819, China.

In this study, convolutional neural network based on Gramian angular field (GAF-CNN) was firstly proposed. The 1-D Raman spectral data was converted into images and used for predicting the biochemical value of blood glucose. 106 sets of blood spectrums were acquired by Fourier transform (FT) Raman spectroscopy. Spectral data ranging from 800 cm to 1800 cm were selected for quantitative analysis of the blood glucose. Data augmentation was used to train neural networks and normalize the Raman spectra. And, we applied principal component analysis (PCA) for dimension reduction and information extraction. The root mean squared error of prediction (RMSEP) are 0.06570 (GADF) and 0.06774 (GASF), the determination coefficient of prediction (R) are 0.99929 (GADF) and 0.99925 (GASF), and the residual predictive deviation of prediction (RPD) are 37.56324 (GADF) and 36.43362 (GASF). GAF-CNN model performed better for predicting of glucose concentration. The GAF-CNN model can be used to establish a calibration model to predict blood glucose concentration.
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http://dx.doi.org/10.1016/j.saa.2022.121189DOI Listing
July 2022
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