Publications by authors named "Zhigang Li"

607 Publications

Letter to the Editor: Comparison of Outcomes Between Additional Esophagectomy After Noncurative Endoscopic Resection and Upfront Esophagectomy for T1N0 Esophageal Squamous Cell Carcinoma.

Ann Surg Oncol 2021 May 6. Epub 2021 May 6.

Department of Thoracic Surgery, Section of Esophageal Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

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http://dx.doi.org/10.1245/s10434-021-10102-1DOI Listing
May 2021

A Highly Efficient Three-Liquid-Phase-Based Enzymatic One-Pot Multistep Reaction System with Recoverable Enzymes for the Synthesis of Biodiesel.

J Agric Food Chem 2021 May 6. Epub 2021 May 6.

School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.

A three-liquid-phase system (TLPS) was developed and used as a novel enzymatic one-pot multistep reaction (EOMR) system. In this system, lipase and phospholipase were enriched in a single liquid phase with a high recovery (ca. 98%) and then used for the simultaneous catalysis of mutually inhibiting and interfering reactions (hydrolysis of phospholipids and glyceride in crude oil). A novel emulsion containing the two dispersed droplets (W/O/W and W/W emulsion structures) could be the key reason for this phenomenon because the emulsion system not only provided a new catalytic interface but also relieved the product inhibition. As a result, the content of free fatty acid (main hydrolysate of the glyceride) and the removal of phospholipid from the crude oil could be increased to 96 and 95%, respectively, within 1 h. The product obtained from the EOMR was directly used in the production of biodiesel via enzymatic esterification, and the content of fatty acid methanol ester could be increased to 93% within 2 h. Furthermore, the enzymes in the middle phase could also be reused, at least for eight rounds without significant loss in catalytic efficiency. Therefore, the TLPS could be considered as an ideal catalytic platform for the EOMR.
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http://dx.doi.org/10.1021/acs.jafc.0c07448DOI Listing
May 2021

Infant Feeding Alters the Longitudinal Impact of Birth Mode on the Development of the Gut Microbiota in the First Year of Life.

Front Microbiol 2021 7;12:642197. Epub 2021 Apr 7.

Department of Epidemiology, The Geisel School of Medicine at Dartmouth, Hanover, NH, United States.

Cesarean-delivered (CD) infants harbor a distinct gut microbiome from vaginally delivered (VD) infants, however, during infancy, the most important driver of infant gut microbial colonization is infant feeding. Earlier studies have shown that breastfeeding is associated with higher levels of health-promoting bacteria such and and via modulation of the immune system, and production of metabolites. As the infant gut matures and solid foods are introduced, it is unclear whether longer duration of breast feeding restore loss of beneficial taxa within the intestinal microbiota of operatively delivered infants. Within the New Hampshire Birth Cohort Study, we evaluated the longitudinal effect of delivery mode and infant feeding on the taxonomic composition and functional capacity of developing gut microbiota in the First year of life. Microbiota of 500 stool samples collected between 6 weeks and 12 months of age (from 229 infants) were characterized by 16S ribosomal RNA sequencing. Shotgun metagenomic sequencing was also performed on 350 samples collected at either 6 weeks or 12 months of age. Among infant participants, 28% were cesarean-delivered (CD) infants and most (95%) initiated breastfeeding within the first six months of life, with 26% exclusively breastfed and 69% mixed-fed (breast milk and formula), in addition to complementary foods by age 1. Alpha (within-sample) diversity was significantly lower in CD infants compared to vaginally delivered (VD) infants ( < 0.05) throughout the study period. Bacterial community composition clustering by both delivery mode and feeding duration at 1 year of age revealed that CD infants who were breast fed for < 6 months were more dissimilar to VD infants than CD infants who breast fed for ≥ 6 months. We observed that breastfeeding modified the longitudinal impact of delivery mode on the taxonomic composition of the microbiota by 1 year of age, with an observed increase in abundance of and with longer duration of breastfeeding among CD infants while there was an increase in for VD infants. Our findings confirm that duration of breastfeeding plays a critical role in restoring a health-promoting microbiome, call for further investigations regarding the association between breast milk exposure and health outcomes in early life.
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http://dx.doi.org/10.3389/fmicb.2021.642197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059768PMC
April 2021

Endoscopic resection with adjuvant treatment versus esophagectomy for early-stage esophageal cancer.

Surg Endosc 2021 Apr 23. Epub 2021 Apr 23.

Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai, China.

Objective: To evaluate the outcome following the strategy of endoscopic R0 resection (ER) plus adjuvant treatment (AT) versus esophagectomy for esophageal squamous cell cancer in T1a invading muscularis mucosa (M3)-T1b stage.

Methods: We evaluated the outcomes of 46 esophageal squamous cell cancer (ESCC) patients with T1aM3-T1b stage who underwent ER + AT from the Esophageal Cancer Endoscopic Therapy Consortium (ECETC) and compared these outcomes to 92 patients who underwent esophagectomy. Propensity score matching (1:2) was used, with overall survival (OS) and relapse-free survival (RFS) being compared between the two groups.

Results: During a median follow-up of 32 months, there were no statistical differences (P = 0.226) in OS between the two groups. The 1-, 2-, and 3-year overall survival in the esophagectomy group was 95%, 91%, and 84%, respectively. There were no mortalities within three years in the ER + AT group. The RFS between the two groups was also not significantly different (P = 0.938). The 1-, 2-, and 3-year RFS of patients in the esophagectomy group was 90%, 90%, and 83%, respectively, while it was 97%, 94%, and 74% in the ER + AT group, respectively. The local recurrence rates between the two groups were not significantly different (P = 0.277).

Conclusions: This first multicenter analysis showed similar outcomes were found regarding OS and RFS between the two groups in T1aM3-T1b stage patients. ER + AT may be considered in high-risk patients or for those who refuse esophagectomy.
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http://dx.doi.org/10.1007/s00464-021-08466-2DOI Listing
April 2021

HS-SPME and GC/MS volatile component analysis of Yinghong No. 9 dark tea during the pile fermentation process.

Food Chem 2021 Apr 2;357:129654. Epub 2021 Apr 2.

Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, China.

Each type of tea has a unique volatile profile due to its variety, processing technologies and origin. Using HS-SPME and GC/MS, we analyzed the changes of volatile components in cultivar Yinghong No. 9 during pile-fermentation every 10 days. A total of 94 compounds showed significant differences during a total of 60 days mainly including alkanes, ketones, esters, terpenes, aromatics and heterocyclic compounds. Interestingly, 13 metabolites were progressively reduced during the first 20 days and remained unchanged in subsequent procedures, while 17 metabolites remained unchanged in the early stage and progressively increased during the last 20 days of pile fermentation, indicating that they are characteristic volatile compounds of raw material sun-dried green tea and dark tea, respectively. β-ionone, phenylethyl alcohol, and a-ionone could be the top three contributed aroma compounds in the final dark tea. Our study provides a theoretical basis for process and quality improvement of Yinghong No. 9.
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http://dx.doi.org/10.1016/j.foodchem.2021.129654DOI Listing
April 2021

Previously identified and unidentified polybrominated biphenyl congeners in serum from people living in an electronic waste dismantling area in China.

Chemosphere 2021 Apr 8;279:130478. Epub 2021 Apr 8.

College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, China; Engineering Research Center of Food Environment and Public Health, Beijing, 100081, China. Electronic address:

The effects of polybrominated biphenyls (PBBs) on human health have previously attracted much attention, but recent studies of PBBs have been focused on BB-153 and a few other congeners. PBB concentrations in serum samples from residents of an area containing an electronic waste dismantling site were determined in this study. The total PBB concentrations (i.e., the sums of the concentrations of the 35 PBB congeners) were 229-1360 ng/g lipid. The BB-153 concentrations were markedly higher in the samples from people living in the electronic waste dismantling area than in samples from people living in a nearby control area. BB-153 was found in all of the samples from the study exposure area but the concentrations were relatively low (0.07-4.70 ng/g lipid). High BB-1 concentrations were found for the first time in serum from people living in both the electronic waste dismantling and control areas. The BB-1 concentrations were 211-1280 ng/g lipid. The retention times of the 35 PBB standards and PCBs (polychlorinated biphenyls) with similar structures were used to predict the retention times of unidentified PBB congeners to allow the PBB distributions in the serum samples to be identified. A total of 26 previously unidentified PBB congeners were identified in the human serum samples. BB-5, BB-35, BB-79, and BB-109 were found in >50% of the samples. The PBB patterns in the serum samples were different from the patterns previously found in serum after a PBB contamination incident in 1973, so the health risks currently posed by PBBs are worth studying.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130478DOI Listing
April 2021

Transferred Subspace Learning Based on Non-negative Matrix Factorization for EEG Signal Classification.

Front Neurosci 2021 24;15:647393. Epub 2021 Mar 24.

School of Computer Science and Technology, Qilu University of Technology (Shandong Academy of Science), Jinan, China.

EEG signal classification has been a research hotspot recently. The combination of EEG signal classification with machine learning technology is very popular. Traditional machine leaning methods for EEG signal classification assume that the EEG signals are drawn from the same distribution. However, the assumption is not always satisfied with the practical applications. In practical applications, the training dataset and the testing dataset are from different but related domains. How to make best use of the training dataset knowledge to improve the testing dataset is critical for these circumstances. In this paper, a novel method combining the non-negative matrix factorization technology and the transfer learning (NMF-TL) is proposed for EEG signal classification. Specifically, the shared subspace is extracted from the testing dataset and training dataset using non-negative matrix factorization firstly and then the shared subspace and the original feature space are combined to obtain the final EEG signal classification results. On the one hand, the non-negative matrix factorization can assure to obtain essential information between the testing and the training dataset; on the other hand, the combination of shared subspace and the original feature space can fully use all the signals including the testing and the training dataset. Extensive experiments on Bonn EEG confirmed the effectiveness of the proposed method.
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http://dx.doi.org/10.3389/fnins.2021.647393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024531PMC
March 2021

Restricted Feeding Resets Endogenous Circadian Rhythm in Female Mice Under Constant Darkness.

Neurosci Bull 2021 Mar 29. Epub 2021 Mar 29.

Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, 100101, China.

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http://dx.doi.org/10.1007/s12264-021-00669-wDOI Listing
March 2021

High-Order Laplacian Regularized Low-Rank Representation for Multimodal Dementia Diagnosis.

Front Neurosci 2021 12;15:634124. Epub 2021 Mar 12.

Department of Radiology and Biomedical Research Imaging Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.

Multimodal heterogeneous data, such as structural magnetic resonance imaging (MRI), positron emission tomography (PET), and cerebrospinal fluid (CSF), are effective in improving the performance of automated dementia diagnosis by providing complementary information on degenerated brain disorders, such as Alzheimer's prodromal stage, i.e., mild cognitive impairment. Effectively integrating multimodal data has remained a challenging problem, especially when these heterogeneous data are incomplete due to poor data quality and patient dropout. Besides, multimodal data usually contain noise information caused by different scanners or imaging protocols. The existing methods usually fail to well handle these heterogeneous and noisy multimodal data for automated brain dementia diagnosis. To this end, we propose a high-order Laplacian regularized low-rank representation method for dementia diagnosis using block-wise missing multimodal data. The proposed method was evaluated on 805 subjects (with incomplete MRI, PET, and CSF data) from the real Alzheimer's Disease Neuroimaging Initiative (ADNI) cohort. Experimental results suggest the effectiveness of our method in three tasks of brain disease classification, compared with the state-of-the-art methods.
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http://dx.doi.org/10.3389/fnins.2021.634124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994898PMC
March 2021

Calmodulin influences MAPK signaling by binding KSR1.

J Biol Chem 2021 Mar 22:100577. Epub 2021 Mar 22.

Department of Laboratory Medicine, National Institutes of Health, 10 Center Dr., Bethesda, MD 20892, United States. Electronic address:

The mitogen activated protein kinase (MAPK) cascade is a fundamental signaling pathway that regulates cell fate decisions in response to external stimuli. Several scaffold proteins bind directly to kinase components of this pathway and regulate their activation by growth factors. One of the best studied MAPK scaffolds is kinase suppressor of Ras1 (KSR1), which is induced by epidermal growth factor (EGF) to translocate to the plasma membrane where it activates ERK. While Ca has been shown to modulate MAPK signaling, the molecular mechanisms by which this occurs are incompletely understood. Here we tested the hypothesis that Ca alters MAPK activity at least in part via KSR1. Using several approaches, including fusion proteins, immunoprecipitation, confocal microscopy and a cell-permeable chemical inhibitor, we investigated the functional interaction between KSR1 and calmodulin. In vitro analysis with pure proteins reveals that calmodulin binds directly to KSR1. Moreover, endogenous calmodulin and KSR1 co-immunoprecipitate from mammalian cell lysates. Importantly, Ca is required for the association between calmodulin and KSR1, both in vitro and in cells. The cell-permeable calmodulin antagonist CGS9343B significantly reduced activation of ERK by EGF in mouse embryo fibroblasts that overexpress KSR1, but not in control cells. Moreover, CGS9343B impaired the ability of EGF to induce KSR1 translocation to the plasma membrane and to stimulate formation of KSR1-ERK and KSR1-pERK complexes in cells. Collectively, our data identify a previously unrecognized mechanism by which the scaffold protein KSR1 couples Ca and calmodulin signaling to the MAPK cascade.
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http://dx.doi.org/10.1016/j.jbc.2021.100577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079274PMC
March 2021

MicroRNA‑1271‑5p alleviates the malignant development of hepatitis B virus‑mediated liver cancer via binding to AQP5.

Mol Med Rep 2021 May 24;23(5). Epub 2021 Mar 24.

Department of Hepatobiliary Surgery, Binzhou Medical University Hospital, Binzhou, Shandong 256600, P.R. China.

Hepatitis B virus (HBV) is a leading cause of liver‑related cancer. Progress has been made on the study of microRNA (miRNA or miR) function in HBV‑related liver cancer. Hence, the objective of the present study was to determine the role and functional mechanism of miR‑1271‑5p in HBV‑associated liver cancer. miR‑1271‑5p and aquaporin 5 (AQP5) expression at the mRNA level were measured by reverse transcription‑quantitative PCR (RT‑qPCR). The levels of hepatitis B e‑antigen (HBeAg), hepatitis B surface antigen (HBsAg) and HBV DNA were assessed by ELISA or qPCR. Cell viability, apoptosis, migration and invasion were detected by Cell Counting Kit‑8, flow cytometry or Transwell assay. The interaction of miR‑1271‑5p and AQP5 was predicted by TargetScan, and verified by dual‑luciferase reporter assay and RNA binding protein immunoprecipitation assay. The protein levels of AQP5, Bax, Bcl‑2, cleaved‑caspase-3 and proliferating cell nuclear antigen were quantified by western blot analysis. Nude mouse tumorigenicity assay was conducted to examine the role of miR‑1271‑5p . miR‑1271‑5p was downregulated, while AQP5 was upregulated in HBV‑related liver cancer cells and tissues. Overexpression of miR‑1271‑5p or AQP5 knockdown inhibited the levels of HBeAg, HBsAg and HBV DNA, blocked cell viability, migration and invasion, and induced apoptosis. AQP5 was confirmed to be a direct target of miR‑1271‑5p, and miR‑1271‑5p exerted its role through targeting AQP5. Overexpression of miR‑1271‑5p impeded tumor growth in vivo by weakening the expression of AQP5. In conclusion, miR‑1271‑5p blocked the progression of HBV‑induced liver cancer by competitively targeting AQP5.
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http://dx.doi.org/10.3892/mmr.2021.12025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986005PMC
May 2021

Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.

J Exp Med 2021 May;218(5)

Department of Hematology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

The oncogene DEK is found fused with the NUP214 gene creating oncoprotein DEK-NUP214 that induces acute myeloid leukemia (AML) in patients, and secreted DEK protein functions as a hematopoietic cytokine to regulate hematopoiesis; however, the intrinsic role of nuclear DEK in hematopoietic stem cells (HSCs) remains largely unknown. Here, we show that HSCs lacking DEK display defects in long-term self-renew capacity, eventually resulting in impaired hematopoiesis. DEK deficiency reduces quiescence and accelerates mitochondrial metabolism in HSCs, in part, dependent upon activating mTOR signaling. At the molecular level, DEK recruits the corepressor NCoR1 to repress acetylation of histone 3 at lysine 27 (H3K27ac) and restricts the chromatin accessibility of HSCs, governing the expression of quiescence-associated genes (e.g., Akt1/2, Ccnb2, and p21). Inhibition of mTOR activity largely restores the maintenance and potential of Dek-cKO HSCs. These findings highlight the crucial role of nuclear DEK in preserving HSC potential, uncovering a new link between chromatin remodelers and HSC homeostasis, and have clinical implications.
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http://dx.doi.org/10.1084/jem.20201974DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992411PMC
May 2021

Cobalt exposure increases the risk of fibrosis of people living near E‑waste recycling area.

Ecotoxicol Environ Saf 2021 Jun 17;215:112145. Epub 2021 Mar 17.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Center for Global Health, School of Public Health, Nanjing Medical University, China. Electronic address:

The toxicity of heavy metals is one of the major public health issues leading to hazardous effects on humans. Many studies focus on the adverse effects on people who were working in or living near E-waste recycling. However, little is known to the sustaining effects of E-waste exposure on human health after the recycling factories were shut down. In the present study, we collected the blood of people living near E‑waste recycling facilities after the recycling factories were closed for 2 years. Eight heavy metals were examined in all blood samples. The results revealed that the blood levels of lead (Pb), nickel (Ni), cobalt (Co), mercury (Hg) were significantly higher in the exposed group than in the reference group, and no difference was observed for copper (Cu), zinc (Zn), stannum (Sn), cadmium (Cd). Transforming growth factor-β (TGF-β) and alpha-smooth muscle actin (α-SMA) were analyzed as the important indicators of fibrosis, which were statistically significantly higher in the exposed group than in the reference group. 8-isoprostane (8-I) and malondialdehyde (MDA) as the biomarkers of oxidative stress (OS) were elevated in the exposed group. Furthermore, both Spearman correlation and multiple linear regression showed that Co was positively correlated with TGF-β, α-SMA and 8-I in the exposed group. Accordingly, we speculate that high concentrations of Co dissolved in the blood may increase the risk of tissue fibrosis through stimulating myofibroblast activation and OS involve in the process, which may provide some potential new hints for the intervention for tissue fibrosis in the future.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112145DOI Listing
June 2021

Morbidity and Mortality of Patients Who Underwent Minimally Invasive Esophagectomy After Neoadjuvant Chemoradiotherapy vs Neoadjuvant Chemotherapy for Locally Advanced Esophageal Squamous Cell Carcinoma: A Randomized Clinical Trial.

JAMA Surg 2021 May;156(5):444-451

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), The First Department of Thoracic Surgery, Peking University Cancer Hospital and Institute, Peking University School of Oncology, Beijing, China.

Importance: Safety and efficacy of neoadjuvant chemoradiotherapy (nCRT) vs neoadjuvant chemotherapy (nCT) for treatment of locally advanced esophageal squamous cell carcinoma (ESCC) remain uncertain given lack of high-level clinical evidence.

Objective: To compare safety and long-term survival of nCRT followed by minimally invasive esophagectomy (MIE) with that of nCT followed by MIE for patients with locally advanced ESCC.

Design, Setting, And Participants: A prospective, multicenter, open-label, randomized clinical trial that compared safety and efficacy of nCRT vs nCT followed by MIE for patients with locally advanced ESCC. From January 1, 2017, to December 31, 2018, 264 patients with ESCC of clinical stages from cT3 to T4aN0 to 1M0 were enrolled. Analysis was performed on an intention-to-treat basis from January 1, 2017, to August 30, 2020.

Interventions: Eligible patients were randomized to the nCRT group (n = 132) or the nCT group (n = 132) by a computer-generated random system. The chemotherapy, based on paclitaxel and cisplatin, was administered to both groups, while 40 Gy of concurrent radiotherapy was added for the nCRT group. At about 6 weeks after neoadjuvant therapy, MIE via thoracoscopy and laparoscopy was performed for the patients in both groups.

Main Outcomes And Measures: The primary outcome was 3-year overall survival. Secondary outcomes included postoperative complications, mortality, postoperative pathologic outcome, recurrence-free survival time, and quality of life.

Results: Among 264 patients (226 men [85.6%]; mean [SD] age, 61.4 [6.8] years), postoperative morbidity was 47.4% in the nCRT group (54 of 114) and 42.6% in the nCT group (46 of 108), with no significant difference between groups (difference, 4.8%; 95% CI, -8.2% to 17.5%; P = .48). Distribution of the severity of complications was similar between the 2 groups based on Clavien-Dindo classification. The 90-day perioperative mortality rate was 3.5% for the nCRT group (4 of 114) and 2.8% for the nCT group (3 of 108) (P = .94). The R0 resection rates were similar between groups (109 of 112 [97.3%] vs 100 of 104 [96.2%]; P = .92). However, patients in the nCRT group had a higher pathologic complete response (residual tumor, 0%) rate (40 of 112 [35.7%] vs 4 of 104 [3.8%]; P < .001) and a higher rate of negative lymph nodes (ypN0, 74 of 112 [66.1%] vs 48 of 104 [46.2%]; P = .03) than those in the nCT group. One-year overall survival using intention-to-treat analysis was 87.1% in the nCRT group (115 of 132) and 82.6% in the nCT group (109 of 132) (P = .30). Furthermore, deaths caused by tumor progression or recurrence were significantly less in the nCRT group than in the nCT group (9 of 132 [6.8%] vs 19 of 132 [14.4%]; P = .046); however, deaths from nontumor causes were similar (8 of 132 [6.1%] vs 4 of 132 [3.0%]; P = .24).

Conclusions And Relevance: Initial results of the trial showed that nCRT followed by MIE has similar safety to and better histopathologic outcome than nCT followed by MIE for treatment of locally advanced ESCC.

Trial Registration: ClinicalTrials.gov Identifier: NCT03001596.
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http://dx.doi.org/10.1001/jamasurg.2021.0133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970392PMC
May 2021

[Application of self-made protective clothing in tracheal intubation for patients with severe respiratory infectious diseases].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 Feb;33(2):241-243

Department of Respiratory, the Third Affiliated Hospital of Gansu University of Chinese Medicine, the First People's Hospital of Baiyin, Baiyin 730900, Gansu, China. Corresponding author: Li Zhigang, Email:

Objective: To explore the effect of self-made protective clothing in tracheal intubation for the patients with respiratory infectious diseases.

Methods: Self-made protective clothing were made by adult model plastic raincoat with sleeve lets and goggles. A prospective randomized controlled study was conducted. Patients with severe respiratory infectious diseases who needed tracheal intubation admitted to the department of intensive medicine of the Third Affiliated Hospital of Gansu University of Chinese Medicine from January 1st 2018 to March 31st 2020 were enrolled. According to the random number table method, they were divided into two groups. The control group was wearing standard protective clothing, while the test group was wearing self-made protective clothing for endotracheal intubation. The wearing time, infection rate of operators and costs of protective clothing were compared between the two groups. The patients were sprayed with trypanosome blue diluent before tracheal intubation, and the whole body of the operator was photographed with fluorescence before wearing self-made protective clothing and after doing tracheal intubation to take off the self-made protective clothing, in order to evaluate the permeability resistance of self-made protective clothing.

Results: A total of 86 patients were enrolled. There were 46 cases in the test group, included 28 cases of influenza A (H1N1) virus infection, 11 cases of influenza B virus infection and 7 cases of adenovirus infection. There were 40 cases in the control group, included 15 cases of H1N1 virus infection, 10 cases of influenza B virus infection, 10 cases of adenovirus infection and 5 cases of unknown pathogen. There was no significant difference in respiratory etiology between the two groups (χ = 3.789, P = 0.435). The wearing protective clothing time of the control group was 11.6 times than that of the test group (minutes: 22.23±1.45 vs. 1.86±0.24, χ = 19.023, P < 0.001). The cost of standard protective clothing was 12.5 times than that of self-made protective clothing (Yuan/set: 500 vs. 40). Fluorescent photography showed that the whole body of the operator was not stained after tracheal intubation, indicating that the protective clothing had good anti permeability and achieved the protective effect. There was no operator infection in the test group and the control group.

Conclusions: Self-made protective clothing has short wearing time, low cost and equivalent isolation effect compared with standard protective clothing, which is worthy of clinical promotion.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200915-00625DOI Listing
February 2021

A population-based predictive model predicting candidate for primary tumor surgery in patients with metastatic esophageal cancer.

J Thorac Dis 2021 Feb;13(2):870-882

Department of Thoracic Surgery, Section of Esophageal Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Background: The survival benefit of primary tumor surgery for metastatic esophageal cancer (mEC) patients has been observed, but methods for discriminating which individual patients would benefit from surgery have been poorly defined. Herein, a predictive model was developed to test the hypothesis that only certain metastatic patients would gain a survival benefit from primary tumor surgery.

Methods: Clinical data for patients with mEC were extracted from the Surveillance, Epidemiology and End Results (SEER) database [2004-2016] and then divided into surgery and no-surgery groups according to whether surgery was performed on the primary tumor. Propensity-score-matching (PSM) was performed to balance the confounding factors. We hypothesized that the patients who had undergone surgery and lived longer than the median cancer-specific-survival (CSS) of the no-surgery group could benefit from surgery. We constructed a nomogram to predict surgery benefit potential based on multivariable logistic-regression analysis using preoperative factors. The predictive performance of the nomogram was evaluated by the area under the receiver operating characteristic (AUC) and calibration curves. The clinical application value of the nomogram was estimated with decision curve analysis (DCA).

Results: A total of 5,250 eligible patients with mEC were identified, and 9.4% [492] received primary tumor surgery. After PSM, CSS for the surgery group was significantly longer [median: 19 9 months; hazard ratio (HR) 0.52, P<0.001] compared with the no-surgery group. Among the surgery group, 69.3% [327] survived >9 months (surgery-beneficial group). The prediction nomogram showed good discrimination both in training and validation sets (AUC: 0.72 and 0.70, respectively), and the calibration curves indicated a good consistency. DCA demonstrated that the nomogram was clinically useful. According to this nomogram, surgery patients were classified into two groups: no-benefit-candidate and benefit-candidate. The benefit-candidate group was associated with longer survival than the no-benefit-candidate group (median CSS: 19 6.5 months, P<0.001). Additionally, there was no difference in survival between the no-benefit-candidate and no-surgery groups (median CSS: 6.5 9 months, P=0.070).

Conclusions: A predictive model was created for the selection of candidates for surgical treatment among mEC patients. This predictive model might be used to select patients who may benefit from primary tumor surgery.
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http://dx.doi.org/10.21037/jtd-20-2347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947545PMC
February 2021

B-Raf autoinhibition in the presence and absence of 14-3-3.

Structure 2021 Mar 8. Epub 2021 Mar 8.

Computational Structural Biology Section, Frederick National Laboratory for Cancer Research in the Laboratory of Cancer ImmunoMetabolism, National Cancer Institute, Frederick, MD 21702, USA; Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel. Electronic address:

Raf-activating mutations are frequent in cancer. In the basal state, B-Raf is autoinhibited by its upstream Ras-binding domain (RBD) and cysteine-rich domain (RBD-CRD) interacting with its kinase domain (KD) and the 14-3-3 dimer. Our comprehensive molecular dynamics simulations explore two autoinhibition scenarios in the presence and absence of the 14-3-3 dimer. When present, the 14-3-3 interaction with B-Raf stabilizes the RBD-CRD-KD interaction, interfering with the KD dimerization. Raf's pSer365 removal fails to induce large disruption. RBD-CRD release promotes KD fluctuations and reorientation for dimerization, consistent with experimental data. In the absence of 14-3-3, our sampled B-Raf conformations suggest that RBD-CRD can block the KD dimerization surface. Our results suggest a B-Raf activation mechanism, whereby one KD monomer is donated by 14-3-3-free B-Raf KD and the other by 14-3-3-bound KD. This mechanism can lead to homo- and heterodimers. These autoinhibition scenarios can transform autoinhibited B-Raf monomers into active B-Raf dimers.
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http://dx.doi.org/10.1016/j.str.2021.02.005DOI Listing
March 2021

Potential interaction between autophagy and auxin during maize leaf senescence.

J Exp Bot 2021 May;72(10):3554-3568

Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

Leaf senescence is important for crop yield as delaying it can increase the average yield. In this study, population genetics and transcriptomic profiling were combined to dissect its genetic basis in maize. To do this, the progenies of an elite maize hybrid Jidan27 and its parental lines Si-287 (early senescence) and Si-144 (stay-green), as well as 173 maize inbred lines were used. We identified two novel loci and their candidate genes, Stg3 (ZmATG18b) and Stg7 (ZmGH3.8), which are predicted to be members of autophagy and auxin pathways, respectively. Genomic variations in the promoter regions of these two genes were detected, and four allelic combinations existed in the examined maize inbred lines. The Stg3Si-144/Stg7Si-144 allelic combination with lower ZmATG18b expression and higher ZmGH3.8 expression could distinctively delay leaf senescence, increase ear weight and the improved hybrid of NIL-Stg3Si-144/Stg7Si-144 × Si-144 significantly reduced ear weight loss under drought stress, while opposite effects were observed in the Stg3Si-287/Stg7Si-287 combination with a higher ZmATG18b expression and lower ZmGH3.8 expression. Thus, we identify a potential interaction between autophagy and auxin which could modulate the timing of maize leaf senescence.
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http://dx.doi.org/10.1093/jxb/erab094DOI Listing
May 2021

Hairpin DNA-Mediated isothermal amplification (HDMIA) techniques for nucleic acid testing.

Talanta 2021 May 5;226:122146. Epub 2021 Feb 5.

Biomaterials Research Center, School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou, 510515, China. Electronic address:

Nucleic acid detection is of great importance in a variety of areas, from life science and clinical diagnosis to environmental monitoring and food safety. Unfortunately, nucleic acid targets are always found in trace amounts and their response signals are difficult to be detected. Amplification mechanisms are then practically needed to either duplicate nucleic acid targets or enhance the detection signals. Polymerase chain reaction (PCR) is one of the most popular and powerful techniques for nucleic acid analysis. But the requirement of costly devices for precise thermo-cycling procedures in PCR has severely hampered the wide applications of PCR. Fortunately, isothermal molecular reactions have emerged as promising alternatives. The past decade has witnessed significant progress in the research of isothermal molecular reactions utilizing hairpin DNA probes (HDPs). Based on the nucleic acid strand interaction mechanisms, the hairpin DNA-mediated isothermal amplification (HDMIA) techniques can be mainly divided into three categories: strand assembly reactions, strand decomposition reactions, and strand creation reactions. In this review, we introduce the basics of HDMIA methods, including the sensing principles, the basic and advanced designs, and their wide applications, especially those benefiting from the utilization of G-quadruplexes and nanomaterials during the past decade. We also discuss the current challenges encountered, highlight the potential solutions, and point out the possible future directions in this prosperous research area.
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http://dx.doi.org/10.1016/j.talanta.2021.122146DOI Listing
May 2021

Sorghum breeding in the genomic era: opportunities and challenges.

Theor Appl Genet 2021 Mar 2. Epub 2021 Mar 2.

Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

Key Message: The importance and potential of the multi-purpose crop sorghum in global food security have not yet been fully exploited, and the integration of the state-of-art genomics and high-throughput technologies into breeding practice is required. Sorghum, a historically vital staple food source and currently the fifth most important major cereal, is emerging as a crop with diverse end-uses as food, feed, fuel and forage and a model for functional genetics and genomics of tropical grasses. Rapid development in high-throughput experimental and data processing technologies has significantly speeded up sorghum genomic researches in the past few years. The genomes of three sorghum lines are available, thousands of genetic stocks accessible and various genetic populations, including NAM, MAGIC, and mutagenised populations released. Functional and comparative genomics have elucidated key genetic loci and genes controlling agronomical and adaptive traits. However, the knowledge gained has far away from being translated into real breeding practices. We argue that the way forward is to take a genome-based approach for tailored designing of sorghum as a multi-functional crop combining excellent agricultural traits for various end uses. In this review, we update the new concepts and innovation systems in crop breeding and summarise recent advances in sorghum genomic researches, especially the genome-wide dissection of variations in genes and alleles for agronomically important traits. Future directions and opportunities for sorghum breeding are highlighted to stimulate discussion amongst sorghum academic and industrial communities.
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http://dx.doi.org/10.1007/s00122-021-03789-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924314PMC
March 2021

The Effectiveness of Ruxolitinib for Acute/Chronic Graft-versus-Host Disease in Children: A Retrospective Study.

Drug Des Devel Ther 2021 22;15:743-752. Epub 2021 Feb 22.

Department of Hematology and Oncology, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing Key Laboratory of Pediatric Hematology Oncology, Key Laboratory of Major Diseases in Children, Ministry of Education, National Key Discipline of Pediatrics, Beijing, People's Republic of China.

Objective: This study aimed to evaluate the effectiveness of Ruxolitinib for acute/chronic graft-versus-host disease in children.

Methods: This study was a retrospective trial. We analyzed the clinical characteristics of children who responded poorly to previous treatment for graft-versus-host disease (GVHD) and received ruxolitinib treatment after allogeneic hematopoietic stem cell transplantation (allo-HSCT) as an additional or replacement therapy.

Results: A total of 53 patients were analyzed: aGVHD and cGVHD. The overall response rate (ORR) to ruxolitinib was 75.5%. The ORR was 64.7% (11/17) in the aGVHD group including 6, 5, and 6 patients with partial responses (PRs), complete responses (CRs), and treatment failure, respectively. The ORR was 80.6% (29/36) in the cGVHD group including 10 with CRs and 19 with PRs. Five and 2 patients showed no response and treatment failure, respectively. Four and 14 patients were GVHD recurrence in aGVHD and cGVHD respectively. A total of 14 patients (39%) discontinued steroids and 8 patients (22.2%) reduced steroids. The incidence of obvious adverse events was 94.1% (16/17) in the aGVHD group, which was higher than that in the cGVHD group. Meanwhile, the prognosis of children with cGVHD was superior to that of children with aGVHD after treatment with ruxolitinib. During the ruxolitinib treatment, only 1 patient suffered a relapse of the primary tumor. Eleven patients also suffered transplantation-associated thrombotic microangiopathy (TA-TMA) after allo-HSCT.

Conclusion: Pediatric patients with GVHD (especially cGVHD) responded well to ruxolitinib treatment. Ruxolitinib can also be used as an alternative treatment for patients with TMA.
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http://dx.doi.org/10.2147/DDDT.S287218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910527PMC
February 2021

Pannexin 1 binds β-catenin to modulate melanoma cell growth and metabolism.

J Biol Chem 2021 Feb 26:100478. Epub 2021 Feb 26.

Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada, N6A 5C1; Department of Oncology, Division of Experimental Oncology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada, N6A 5C1. Electronic address:

Melanoma is the most aggressive skin malignancy with increasing incidence worldwide. Pannexin1 (PANX1), a member of the pannexin family of channel-forming glycoproteins, regulates cellular processes in melanoma cells including proliferation, migration, and invasion/metastasis. However, the mechanisms responsible for coordinating and regulating PANX1 function remain unclear. Here, we demonstrated a direct interaction between the C-terminal region of PANX1 and the N-terminal portion of β-catenin, a key transcription factor in the Wnt pathway. At the protein level, β-catenin was significantly decreased when PANX1 was either knocked down or inhibited by two PANX1 blockers, Probenecid and Spironolactone. Immunofluorescence imaging showed a disrupted pattern of β-catenin localization at the cell membrane in PANX1-deficient cells, and transcription of several Wnt target genes, including MITF, was suppressed. In addition, a mitochondrial stress test revealed that the metabolism of PANX1-deficient cells was impaired, indicating a role for PANX1 in the regulation of the melanoma cell metabolic profile. Taken together, our data show that PANX1 directly interacts with β-catenin to modulate growth and metabolism in melanoma cells. These findings provide mechanistic insight into PANX1-mediated melanoma progression and may be applicable to other contexts where PANX1 and β-catenin interact as a potential new component of the Wnt signaling pathway.
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http://dx.doi.org/10.1016/j.jbc.2021.100478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027267PMC
February 2021

The varying driving forces of urban land expansion in China: Insights from a spatial-temporal analysis.

Sci Total Environ 2021 Apr 8;766:142591. Epub 2020 Oct 8.

School of Geography and Planning, Sun Yat-Sen University, 135 Xingang Xi Road, Guangzhou, Guangdong, 510275, China.

The impacts of socioeconomic development on urban land expansion in China vary across space and time; however, comprehensive investigation of this issue remains scarce in the existing literature. This study used a geographically and temporally weighted regression model (GTWR) to examine the spatiotemporally heterogeneous impacts of socioeconomic factors on urban land expansion in China using a newly available annual urban land-use dataset from 2000 to 2015. We found that although the eastern region has maintained its leading role (53.79%) in terms of urban expansion, the share of the central (20.34%) and western (16.13%) regions is gradually increasing. Cities with a higher administrative status tended to expand more rapidly; however, increasingly expansion has also taken place in the prefecture-level cities in recent years. We further found that Gross domestic product (GDP) growth, population density, and capital investment positively contributed to the expansion, although the directions and strengths of association between these factors and urban expansion varied across space and time. Industrial structure and foreign direct investment (FDI) showed a similar variation change trend, with the number of cities evidencing a negative relationship rapidly expanding and increasingly being seen not just in northwest China but also in the southeast during the study period. We also found that the correlation between public finance expenditure and urban expansion presented significant north-south differentiation. It is worth noting that governmental behavior plays a significant role in driving urban land expansion. Our empirical study confirmed the spatiotemporal heterogeneous effects of socioeconomic factors on urban expansion in China, providing useful insights for city governments and urban planners.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142591DOI Listing
April 2021

Surgical treatment of esophageal cancer with anomaly of the aortic arch and its branches.

Esophagus 2021 Feb 4. Epub 2021 Feb 4.

Section of Esophageal Surgery, Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiaotong University, No. 241 West Huaihai Road, Shanghai, 200030, China.

Background: Anomalies of the aortic arch and its branches rarely develop. The surgery for esophageal cancer may be challenging with the presence of these anomalies. This study is aimed to analyze the influence of these variations during the esophagectomy.

Methods: A total of 21 patients with aortic arch and brachiocephalic vessel variations were retrospectively identified from 2013 to 2019. Anomalies were distributed: 15 patients with left-sided aortic arch combined with aberrant right subclavian artery (LAA + ARSA), 2 right-sided aortic arch with mirror-image arch branches (RAA + MIAB), and 4 right-sided aortic arch combined with aberrant left subclavian artery (RAA + ALSA). Perioperative characteristics and long-term survival were analyzed.

Results: Tumors were mostly located in the upper and middle thorax (42.9% and 47.6%, respectively). Of the 15 patients with LAA + ARSA, 13 underwent McKeown esophagectomy and 2 via transhiatal approach. Left thoracotomy was performed on all 6 patients with RAA. The R0 resection rate was 90.5% (19/21). Recurrent laryngeal nerve (RLN) injury occurred in 2 patients. Two patients died within 30 days postoperatively. Lymph node yield was 23.6 ± 1.2, with a metastasis rate of 38.1% (8/21). The median follow-up time was 18 months. Recurrence occurred in 6 patients (11-35 months) and 4 patients died after recurrence with a median time to death of 21 months (8-47 months).

Conclusion: For resectable esophageal cancer combined with aortic arch and its branches anomalies, satisfactory surgical results can be obtained under careful preoperative evaluation and reasonable surgical approach selection.
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http://dx.doi.org/10.1007/s10388-020-00810-0DOI Listing
February 2021

is essential for the quiescence and maintenance of adult hematopoietic stem cells under stress.

iScience 2021 Feb 7;24(2):102039. Epub 2021 Jan 7.

Department of Hematology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.

Zinc finger protein 521 (), a quiescent hematopoietic stem cell (HSC)-enriched transcription factor, is involved in the self-renewal and differentiation of fetal liver HSC. However, its role in adult hematopoiesis remains elusive. Here, we found that deletion did not inhibit adult hematopoiesis under homeostatic conditions. In contrast, -null chimeric mice showed significantly reduced pool size of HSC and hematopoietic progenitor cells associated with increased apoptosis and loss of quiescence. Competitive serial transplantation assays revealed that regulates HSC self-renewal and differentiation under regenerative stress. Mechanistically, transcriptionally repressed expression by increasing H3K9ac and decreasing H3K9me3 levels in its promoter. Knockdown of inhibited the hyper-activated NF-κB pathway and reversed the loss of quiescence in -null HSC under stress. Thus, our results reveal a previously unrecognized role for as critical regulator of quiescence and self-renewal of HSC in adult hematopoiesis mediated at least partly by controlling expression.
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http://dx.doi.org/10.1016/j.isci.2021.102039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822949PMC
February 2021

Occurrence and Distribution of Disinfection Byproducts in Domestic Wastewater Effluent, Tap Water, and Surface Water during the SARS-CoV-2 Pandemic in China.

Environ Sci Technol 2021 04 1;55(7):4103-4114. Epub 2021 Feb 1.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People's Republic of China.

Intensified efforts to curb transmission of the Severe Acute Respiratory Syndrome Coronavirus-2 might lead to an elevated concentration of disinfectants in domestic wastewater and drinking water in China, possibly resulting in the generation of numerous toxic disinfection byproducts (DBPs). In this study, the occurrence and distribution of five categories of DBPs, including six trihalomethanes (THMs), nine haloacetic acids (HAAs), two haloketones, nine nitrosamines, and nine aromatic halogenated DBPs, in domestic wastewater effluent, tap water, and surface water were investigated. The results showed that the total concentration level of measured DBPs in wastewater effluents (78.3 μg/L) was higher than that in tap water (56.0 μg/L, = 0.05), followed by surface water (8.0 μg/L, < 0.01). Moreover, HAAs and THMs were the two most dominant categories of DBPs in wastewater effluents, tap water, and surface water, accounting for >90%, respectively. Out of the regulated DBPs, none of the wastewater effluents and tap water samples exceeded the corresponding maximum guideline values of chloroform (300 μg/L), THM4 (80 μg/L), NDMA (100 ng/L), and only 2 of 35 tap water samples (67.6 and 63.3 μg/L) exceeded the HAA5 (60 μg/L) safe limit. HAAs in wastewater effluents showed higher values of risk quotient for green algae. This study illustrates that the elevated use of disinfectants within the guidance ranges during water disinfection did not result in a significant increase in the concentration of DBPs.
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http://dx.doi.org/10.1021/acs.est.0c06856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875339PMC
April 2021

Clinical analysis of chronic active EBV infection with coronary artery dilatation and a matched case-control study.

Orphanet J Rare Dis 2021 01 28;16(1):50. Epub 2021 Jan 28.

Beijing Key Laboratory of Pediatric Hematology Oncology; National Key Discipline of Pediatrics (Capital Medical University); Key Laboratory of Major Diseases in Children, Ministry of Education; Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, People's Republic of China.

Objective: To investigate the clinical characteristics, treatment, prognosis and risk factors for chronic active Epstein-Barr Virus infection (CAEBV) associated with coronary artery dilatation (CAD) in children.

Methods: Children with CAEBV associated with CAD hospitalized at Beijing Children's Hospital, Capital Medical University from March 2016 to December 2019 were analyzed. Children with CAEBV without CAD were selected as the control group and matched by sex, age, treatment and admission time. The clinical manifestations, laboratory and ultrasound examinations, treatment and prognosis of the children were collected in both groups.

Results: There were 10 children with CAEBV combined with CAD, including 6 males and 4 females, accounting for 8.9% (10/112) of CAEBV patients in the same period, with an onset age of 6.05 (2.8-14.3) years. The median follow-up time was 20 (6-48) months. All the patients had high copies of EBV-DNA in whole blood [1.18 × 10 (1.90 × 10-3.96 × 10) copies/mL] and plasma [1.81 × 10 (1.54 × 10-1.76 × 10) copies/mL], and all biopsy samples (bone marrow, lymph nodes or liver) were all positive for Epstein-Barr virus-encoded small RNA. Among the 10 children, 8 had bilateral CAD, and 2 patients had unilateral CAD. After diagnosis, 7 children were treated with L-DEP chemotherapy in our hospital. After chemotherapy, four patients underwent allogeneic hematopoietic stem cell transplantation (HSCT). The others were waiting for HSCT. At the time of the last patients follow up record, the CAD had returned to normal in 3 patients, and the time from the diagnosis of CAD to recovery was 21 (18-68) days. LDH, serum ferritin, TNF-α and IL-10 levels were statistically significantly different between the two groups (P = 0.009, 0.008, 0.026 and 0.030). There were no significant differences in survival rate between the two groups (P = 0.416).

Conclusion: The incidence of CAEBV with CAD was low. CAEBV with CAD did not influence the prognosis. Patients who had high LDH, serum ferritin, TNF-α, and IL-10 levels early in their illness were more likely to develop CAD.
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http://dx.doi.org/10.1186/s13023-021-01689-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7845094PMC
January 2021

Integration and Potential Application Ability of Culturable Functional Microorganism in Oil Tea Camellia.

Indian J Microbiol 2021 Mar 1;61(1):1-9. Epub 2020 Sep 1.

Hunan Academy of Forestry, Changsha, 410004 China.

Oil tea Camellia is a major woody oil plant, which has a positive influence on alleviating the contradiction between supply and demand of edible oil in China. Microbial fertilizer for Oil tea Camellia is urgently needed in current production, and it is of great significance to improve the yield and quality. Culturable functional microorganisms are the basis of research and development for microbial fertilizer. In this study, culturable microorganisms which had abilities of antagonism, growth promotion, phosphorus solubility, nitrogen fixation and drought resistance, were integrated from oil tea literature. And the strains potential application ability were evaluated in terms of functionality, safety and adaptability, culture characteristics, suitable conditions and colonization or infection ability of strains. The results showed that the strains with strongest antagonistic ability were s D2WM and Y13. BbTK-01 and FJMa201101 had the strongest insect resistant ability. and can promote oil tea fastest growth. Phosphorus dissolving ability of NC285 and 6-Y-09 were strongest. The strains with strongest Nitrogen fixing ability were N7-3 and B7-7, and the strains with strongest improving drought resistance ability were and Comprehensive evaluation of strains showed that Y13 and N7-3 had a good applied potential ability. This study would save time-consuming of isolate, purify and identify repetitively for the researchers of functional bacteria of oil tea Camellia. Meanwhile it provides a research basis for selecting targeted strains and constructing the combination of functional strains, therefore provides data support for fertilizer efficiency.
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http://dx.doi.org/10.1007/s12088-020-00904-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810811PMC
March 2021

High concentration of blood cobalt is associated with the impairment of blood-brain barrier permeability.

Chemosphere 2021 Jun 7;273:129579. Epub 2021 Jan 7.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China; Center for Global Health, School of Public Health, Nanjing Medical University, China. Electronic address:

Excess heavy metals can lead to many kinds of adverse effects in human. The present study is designed to investigate whether the internal excess burden of heavy metals relate to the disturbance of the Blood-brain-barrier (BBB) and oxidative stress (OS) in subjects, and identify specific metallic constituents responsible for the disturbance. We collected the blood from recruited 122 subjects for our comparison study, 69 were living at an area near e-waste dismantling factories (exposed group), who have higher levels of heavy metals in the body; and others were in a chosen reference area (reference group), who were the general residents in city, in December 2017 in Taizhou, Zhejiang province. The analyses showed that the concentrations of altogether 4 metals, including nickel (Ni), cobalt (Co), mercury (Hg) and stannum (Sn), and the triggers of BBB disruption (Apolipoprotein E4 and matrix-metalloproteinase-9), indicators of BBB (Myelin basic protein, serotonin and dopamine) and biomarkers of OS (Malondialdehyde and 8-isoprostane) were statistically significant higher in exposed group than in reference group. Moreover, they are also significantly positively correlated with each other. Among all the metals, both Pearson correlation and multiple linear regression showed Co was positive correlated with almost all biomarkers. Considering the explicit correlation between Co and BBB permeability, we speculated that high burden of Co in blood may have a connection with neurodegenerative diseases, which propose a requirement for constructing the environmental criteria for Co and might provide a potential new hint for the intervention of dementia.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129579DOI Listing
June 2021

Changes in photosynthetic traits and their responses to increasing fertilization rates in soybean (Glycine max (L.) Merr.) during decades of genetic improvement.

J Sci Food Agric 2021 Jan 25. Epub 2021 Jan 25.

Soybean Research Institute, Shenyang Agricultural University, Shenyang, China.

Backround: Changes in photosynthetic traits (PTs) during the long-term genetic improvement of soybean (Glycine max (L.) Merr.) yield have been studied, but detailed information on whether PT responses to environmental stress have improved, and their correlations with seed yield, are still unknown. Our objectives were to describe the changes in soybean PTs - leaf area index (LAI), leaf chlorophyll content (Chl), net photosynthetic rate (P ), stomatal conductance (g ), and transpiration rate (E) - during decades of genetic improvement, and to detect whether the responses to increasing fertilizer application rates (FRs) of the PTs of 13 different soybean cultivars released in various decades differed.

Results: All of the soybean PTs increased significantly along with the year in which each cultivar was released, under different FR treatments, indicating that PTs have improved during decades of genetic breeding. Medium FR (nitrogen) treatment (150 kg ha ) increased PT values, to different extents, at all the investigated growth stages. Leaf area index, Chl, and P of the old and middle cultivar groups at the full bloom (R2), full seed (R6), and beginning maturity (R7) stages decreased significantly under high FR treatment (300 kg ha ) compared with the medium FR treatment. The former had no effect on any of the PTs of new cultivar group, or had promotive effects. Thus, the photosynthetic capacities of the new cultivars are more tolerant to high FR-related stress than older cultivars.

Conclusions: The photosynthetic capacities, and tolerance to high FR-related stress, of soybean cultivars that were released in different years improved after long-term genetic breeding. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11117DOI Listing
January 2021