Publications by authors named "Zhifeng Zhang"

162 Publications

--Peroxo Species Formed in the Bulk of Silicate Cathodes.

Angew Chem Int Ed Engl 2021 Feb 23. Epub 2021 Feb 23.

Ningbo Institute of Materials Technology and Engineering CAS: Ningbo Institute of Industrial Technology Chinese Academy of Sciences, Institute of New Energy, 1219 Zhongguan West Road, 315201, Ningbo, CHINA.

Oxygen redox in Li-rich oxides may boost the energy-density of lithium-ion batteries by incorporating oxygen chemistry in solid cathodes. However, oxygen redox in the bulk usually entangles with voltage hysteresis and oxygen release, resulting in a prolonged controversy in literature on oxygen transformation. Here, we report spectroscopic evidence of peroxo species formed and confined in silicate cathodes amid oxygen redox at high-voltage, accompanied with Co 2+ /Co 3+ redox dominant at low-voltage. First-principles calculations reveal that localized electrons on dangling oxygen drive the O-O dimerization. The covalence between the binding cation and the O-O dimer determines the degree of electron transfer in oxygen transformation. Dimerization induces irreversible structural distortion and slow kinetics. But peroxo formation can minimize the voltage drop and volume expansion in cumulative cationic and anionic redox. These findings offer insights into oxygen redox in the bulk for rational designs of high energy-density cathodes.
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http://dx.doi.org/10.1002/anie.202100730DOI Listing
February 2021

Risk of fatigue with PARP inhibitors in cancer patients: a systematic review and meta-analysis of 29 phase II/III randomized controlled trials.

J Chemother 2021 Feb 15:1-10. Epub 2021 Feb 15.

College of Pharmacy, Southwest Minzu University, Chengdu, Sichuan, P.R.China.

Fatigue was a frequently reported adverse event associated with the use of PARP inhibitors. We performed a systematic review and meta-analysis to fully investigate the fatigue of PARP inhibitors in cancer patients. Databases were searched for randomized controlled trials (RCTs) treated with PARP inhibitors till July 2020. Twenty-nine RCTs and 9479 patients were included. This meta-analysis suggests that the use of PARP inhibitors significantly increase the risk of developing all-grade (RR, 1.25; 95%CI, 1.20-1.31;  < 0.00001; I = 48%) and high-grade fatigue (RR, 1.92; 95%CI, 1.51-2.45;  < 0.00001; I = 11%). Veliparib was associated with a relatively lower risk of fatigue. Patients with ovarian cancer tend to be associated with a higher risk of fatigue than those with non-ovarian cancer. Longer duration of therapy was associated with a higher risk of all-grade fatigue. Patients receiving PARP inhibitor monotherapy tends to be associated with a higher risk of all-grade fatigue than those receiving combination treatment.
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http://dx.doi.org/10.1080/1120009X.2021.1884797DOI Listing
February 2021

Fabrication of PDMS microfluidic devices using nanoclay-reinforced Pluronic F-127 as a sacrificial ink.

Biomed Mater 2021 Feb 11. Epub 2021 Feb 11.

Penn State, W313 MSC, University Park, Pennsylvania, 16802, UNITED STATES.

Three-dimensional (3D) printing technology is increasingly being employed in biochemical as well as clinical applications and more importantly in fabrication of microfluidic devices. However, the microfluidic community mainly relies on photolithography for fabrication of a defined mask, which is both tedious and expensive requiring clean room settings as well as limited to the generation of two-dimensional (2D) features. In this work, we 3D printed nanoclay-reinforced Pluronic ink as a sacrificial material, which exhibited shear thinning behavior and superior printability allowing the fabrication of unsupported or overhanging templates of channels with uniform diameter and circular cross-sections. To highlight the potential and effectiveness of the presented approach, we fabricated a human blood vessel-on-a-chip model with curved as well as straight channels. These channels were then lined with Human Umbilical Vein Endothelial cells (HUVECs) and subjected to a dynamic culture for 10 days to explore the effect of shear stress on HUVEC morphology based on the location of HUVECs in the devices. Overall, we presented a highly affordable, useful, and practical approach in fabrication of closed microfluidic channels in PDMS based devices, which holds great potential for numerous applications, such as but not limited to tissue/organ-on-chip, microfluidics, point-of-care devices and drug screening platforms.
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http://dx.doi.org/10.1088/1748-605X/abe55eDOI Listing
February 2021

Identification of the Key Residues of the Uridine Diphosphate Glycosyltransferase 91D2 and its Effect on the Accumulation of Steviol Glycosides in .

J Agric Food Chem 2021 Feb 7;69(6):1852-1863. Epub 2021 Feb 7.

Agronomy College, Sichuan Agricultural University, Chengdu 611130, China.

Stevia ( Bertoni) possesses substantial value for its unique sweet compounds-steviol glycosides (SGs). In the metabolic glycosylation grid of SGs, SrUGT91D2 has been shown to catalyze formation of 1,2-β-d-glucoside linkages at the C- and C-positions and play a crucial role in the synthesis of SGs, including the formation of stevioside (ST), rebaudioside E (RE), and rebaudioside D (RD). However, the key residues of the SrUGT91D2 enzyme and how SrUGT91D2 affects the accumulation of SGs in remain unclear. In the present study, cloning and functional analysis of full-length gene sequences were performed in 10 different genotypes with divergent SG compositions. After sequence analysis, it was found that most of the sequences of this gene (more than 50%) in each genotype were consistent with the allele, which has been reported to exert catalytic activity on 1,2-β-d-glucoside. Moreover, six variants (, , , and ) of this gene were obtained, and their activities were identified. Although there were some differences among these variants, the only type of mutation was partial base substitution at a very low level. In addition, the expression analysis of in each genotype showed that the expression level of the gene was significantly different among genotypes, and a significant positive correlation was found between the content of RD (which was closely influenced by SrUGT91D2) and the expression level of in each genotype (correlation coefficient = 0.91). Thus, it was indicated that was relatively conserved in , and the differential effect of on the accumulation of related SGs mainly derived from its expression level. Furthermore, based on homologous modeling and molecular docking analysis, T84, T144, A194, S284, E285, V286, G365, E369, R404, and G409 were predicted to be key residues in the glucosylation of SGs by SrUGT91D2. After site-mutation and enzyme assays, it was confirmed that T84, T144, R404, A194, and G409 are the key residues in the SrUGT91D2 protein, especially T144 and G409. This work provided valuable information for understanding the structure-activity relationship of the SrUGT91D2 protein and the molecular mechanism of SG accumulation in stevia.
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http://dx.doi.org/10.1021/acs.jafc.0c07066DOI Listing
February 2021

A novel transcription factor MRPS27 up-regulates the expression of sqr, a key gene of mitochondrial sulfide metabolism in echiuran worm Urechis unicinctus.

Comp Biochem Physiol C Toxicol Pharmacol 2021 May 5;243:108997. Epub 2021 Feb 5.

Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. Electronic address:

Hydrogen sulfide is a natural, widely distributed, poisonous substance and sulfide: quinone oxidoreductase (SQR) is responsible for oxidizing hydrogen sulfide to less toxic sulfur compounds. The increase of SQR mRNA level is an important mechanism for organisms to adapt to hydrogen sulfide-rich environments. However, its transcriptional regulation mechanism is not very clear. In this study, a mitochondrial 28S ribosomal protein S27 (MRPS27), which has never been reported as a transcription factor, was screened by yeast one-hybrid experiment from the echiuran worm Urechis unicinctus, a benthic organism living in marine sediments. Western blotting indicated that UuMRPS27 contents increased significantly in the nuclear extract of hindgut under exposed to 150 μM sulfide. ChIP and EMSA assays demonstrated that UuMRPS27 did bind to the sqr proximal promoter, the key binding sequence was CTAGAG (+12 to +17 of the promoter) detected by DNase I footprinting assay as well as transient transfection experiments. Furthermore, UuMRPS27, as a transcription activator, exhibited the highest transcription activity compared with other reported sqr transcription factors. Our data revealed for the first time the role of MRPS27 acting as a transcription factor which expanded the understanding of sqr transcriptional regulation in sulfide metabolism mechanism.
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http://dx.doi.org/10.1016/j.cbpc.2021.108997DOI Listing
May 2021

Microstructures and Macrosegregation of Al-Zn-Mg-Cu Alloy Billet Prepared by Uniform Direct Chill Casting.

Materials (Basel) 2021 Feb 3;14(4). Epub 2021 Feb 3.

General Research Institute for Non-Ferrous Metals, Beijing 100088, China.

In this study, large-sized Al-Zn-Mg-Cu alloy billets were prepared by direct chill casting imposed with annular electromagnetic stirring and intercooling; a process named uniform direct chill casting. The effects of uniform direct chill casting on grain size and the alloying element distribution of the billets were investigated and compared with those of the normal direct chill casting method. The results show that the microstructures were refined and the homogeneity of the alloying elements distribution was greatly improved by imposing the annular electromagnetic stirring and intercooling. In uniform direct chill casting, explosive nucleation can be triggered, originating from the mold wall and dendrite fragments for grain refinement. The effects of electromagnetic stirring on macrosegregation are discussed with consideration of the centrifugal force that drives the movement of melt from the central part towards the upper-periphery part, which could suppress the macrosegregation of alloying elements. The refined grain can reduce the permeability of the melt in the mushy zone that can restrain macrosegregation.
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http://dx.doi.org/10.3390/ma14040708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913293PMC
February 2021

Association of HSS score and mechanical alignment after primary TKA of patients suffering from constitutional varus knee that caused by combined deformities: a retrospective study.

Sci Rep 2021 Feb 4;11(1):3130. Epub 2021 Feb 4.

Department of Orthopaedics, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.

For pre-operative osteoarthritis (OA) patients with varus knee, previous studies showed inconsistent results. Therefore, we conducted this study to better identify the association of Hospital for Special Surgery (HSS) score and mechanical alignment. 44 patients (51 knees) with constitutional varus knee caused by combined deformities (LDFA (lateral distal femoral angle) > 90°and MPTA (medial proximal tibial angle) < 85°)) were selected and analyzed with a mean follow-up period of 14 months after total knee arthroplasty (TKA). From January 2015 to December 2016, patients were collected consecutively after primary TKA. After filtering, fifty-one knees (44patients) were analyzed with a mean follow-up period of 14 months. All patients were divided into two groups based on post-operative hip-knee-ankle (HKA) acute angle: varus mechanical alignment (VMA) group (HKA < - 3°) and neutral mechanical axis (NMA) group (- 3° ≤ HKA ≤ 3°). 30 knees were included in the NMA group, and 21 knees in the VMA group. Comparisons of HSS between NMA group and VMA group were performed. After adjusting for age and Body Mass Index (BMI) confounders, Compared with NMA group, the HSS score in VMA group decreased by 0.81 units (95% CI, - 3.37 to 1.75) p = 0.5370). For pre-operative constitutional varus knee caused by combined deformities in chinese populations, no significant association between post-operative lower limb mechanical alignment and HSS score was found.
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http://dx.doi.org/10.1038/s41598-021-81285-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862289PMC
February 2021

Oligosaccharides from Polygonatum Cyrtonema Hua: Structural characterization and treatment of LPS-induced peritonitis in mice.

Carbohydr Polym 2021 Mar 10;255:117392. Epub 2020 Nov 10.

State Key Laboratory of Quality Research in Chinese Medicine, Faculty of Chinese Medicine, Macau University of Science and Technology, Macau, China; Faculty of Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, China. Electronic address:

Fructooligosaccharide was isolated from Polygonatum Cyrtonema Hua (PFOS) for the first time. Structure characterized using FT-IR, MALDI-TOF-MS, NMR, AFM, and TEM, indicated that PFOS was graminan-type fructan with a degree of polymerization ranging from 5 to 10. A murine model of lipopolysaccharide (LPS)-induced peritonitis was used to evaluate the in vivo anti-inflammatory and lung protective efficacy of PFOS. The result shown that pretreatment with PFOS (1.0 mg/mL) in peritonitis-induced mice could significantly inhibit the level of pro-inflammatory cytokines (TNF-α, IL-1β) in serum (P < 0.001), increase mice survival rate from 12.5 % to 54 % (P < 0.05), and alleviated lung injury through ameliorating the damage of the pulmonary cellular architecture and reducing inflammatory monocyte accumulation in lung tissue. This effect of oligosaccharides could explain the traditional usage of P. cyrtonema as a tonic medicine for respiratory problems and it could be used as a potential natural ingredient with anti-inflammatory activity.
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http://dx.doi.org/10.1016/j.carbpol.2020.117392DOI Listing
March 2021

Identification of the neuropeptide precursor genes potentially involved in the larval settlement in the Echiuran worm Urechis unicinctus.

BMC Genomics 2020 Dec 14;21(1):892. Epub 2020 Dec 14.

Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.

Background: In marine invertebrate life cycles, which often consist of planktonic larval and benthonic adult stages, settlement of the free-swimming larva to the sea floor in response to environmental cues is a key life cycle transition. Settlement is regulated by a specialized sensory-neurosecretory system, the larval apical organ. The neuroendocrine mechanisms through which the apical organ transduces environmental cues into behavioral responses during settlement are not fully understood yet.

Results: In this study, a total of 54 neuropeptide precursors (pNPs) were identified in the Urechis unicinctus larva and adult transcriptome databases using local BLAST and NpSearch prediction, of which 10 pNPs belonging to the ancient eumetazoa, 24 pNPs belonging to the ancient bilaterian, 3 pNPs belonging to the ancient protostome, 9 pNPs exclusive in lophotrochozoa, 3 pNPs exclusive in annelid, and 5 pNPs only found in U. unicinctus. Furthermore, four pNPs (MIP, FRWamide, FxFamide and FILamide) which may be associated with the settlement and metamorphosis of U. unicinctus larvae were analysed by qRT-PCR. Whole-mount in situ hybridization results showed that all the four pNPs were expressed in the region of the apical organ of the larva, and the positive signals were also detected in the ciliary band and abdomen chaetae. We speculated that these pNPs may regulate the movement of larval cilia and chaeta by sensing external attachment signals.

Conclusions: This study represents the first comprehensive identification of neuropeptides in Echiura, and would contribute to a complete understanding on the roles of various neuropeptides in larval settlement of most marine benthonic invertebrates.
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http://dx.doi.org/10.1186/s12864-020-07312-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7737342PMC
December 2020

Research on Microstructure and Mechanical Properties of Rheological Die Forging Parts of Al-6.54Zn-2.40Cu-2.35Mg-0.10Zr(-Sc) Alloy.

Materials (Basel) 2020 Dec 8;13(24). Epub 2020 Dec 8.

National Engineering & Technology Research Center for Non-ferrous Metal Matrix Composites, GRINM Group Co., Ltd., No.11, Xingke East Street, Yanqi Economic Development Zone, Huairou District, Beijing 101407, China.

High-strength aluminum alloy (mainly refers to the 7xxx series) is the optimum material for lightweight military equipment. However, this type of aluminum alloy is a wrought aluminum alloy. If it is directly formed by traditional casting methods, there will inevitably be problems such as coarseness, unevenness, looseness, and hot cracking in the structure, which will greatly affect the final performance of the part. Based on the internal cooling with annular electromagnetic stirring (IC-AEMS) method, a new technology of rheological die forging is developed in this paper, and the scale-reduced parts of a brake hub of Al-6.54Zn-2.40Cu-2.35Mg-0.10Zr aluminum alloy were prepared. The influence of IC-AEMS and the addition of rare element Sc on the structure and mechanical properties of the parts was studied. An optical microscope and scanning electron microscope (SEM) were used to observe the microstructure evolution, energy dispersive spectroscopy (EDS) was used to analyze the phase distribution and composition, and the mechanical properties of the parts were tested by uniaxial tensile tests. The results show that the addition of Sc element can effectively refine the grains and improve the strength and elongation of the material; the application of IC-AEMS improves the cooling rate of the melt, increases the effective nucleation rate, and the grains are further refined. Through process optimization, scale-reduced parts of a brake hub with good formability and mechanical properties can be obtained, the ultimate tensile strength is 597.2 ± 3.1 MPa, the yield strength is 517.8 ± 4.3 MPa, and the elongation is 13.7 ± 1.3%.
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http://dx.doi.org/10.3390/ma13245591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762574PMC
December 2020

Aspiration-assisted freeform bioprinting of prefabricated tissue spheroids in a yield-stress gel.

Commun Phys 2020 16;3. Epub 2020 Oct 16.

Engineering Science and Mechanics Department, Penn State University, 212 Earth-Engineering Sciences Bldg., University Park, PA 16802, USA.

Bioprinting of cellular aggregates, such as tissue spheroids, to form three-dimensional (3D) complex-shaped arrangements, has posed a major challenge due to lack of robust, reproducible and practical bioprinting techniques. Here, we demonstrate 3D aspiration-assisted freeform bioprinting of tissue spheroids by precisely positioning them in self-healing yield-stress gels, enabling the self-assembly of spheroids for fabrication of tissues. The presented approach enables the traverse of spheroids directly from the cell media to the gel and freeform positioning of the spheroids on demand. We study the underlying physical mechanism of the approach to elucidate the interactions between the aspirated spheroids and the gel's yield-stress during the transfer of spheroids from cell media to the gel. We further demonstrate the application of the proposed approach in the realization of various freeform shapes and self-assembly of human mesenchymal stem cell spheroids for the construction of cartilage and bone tissues.
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http://dx.doi.org/10.1038/s42005-020-00449-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695349PMC
October 2020

Hierarchical AgPO@ZnInS nanoscoparium: An innovative Z-scheme photocatalyst for highly efficient and predictable tetracycline degradation.

J Colloid Interface Sci 2021 Mar 5;586:708-718. Epub 2020 Nov 5.

Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle, Nanchang Hangkong University, Nanchang 330063, Jiangxi Province, People's Republic of China.

Z-scheme photocatalyst preserved with superior oxidicability is an innovative photocatalyst system that can be used for efficient photocatalytic detoxification of antibiotics. In this study, Z-scheme AgPO@ZnInS photocatalyst was constructed by decorating AgPO nanoparticles on ZnInS nanoscopariums. ZnInS nanoscopariums were prepared by self-templated strategy and given hierarchical structures. The hierarchical AgPO@ZnInS provides more active sites for generating photogenerated carriers and large surface area for capturing tetracycline. The study results show that AgPO@ZnInS performed excellently well in the photocatalytic degradation of tetracycline and also in protecting AgPO nanoparticles from photo-corrosion. The highest removal efficiency (up to 92.3%) was achieved from the optimal composites of AgPO and ZnInS. In stability tests, AgPO@ZnInS did not reduce the photocatalytic activity of degrading tetracycline after five successive runs. Active radical identification proves that the transfer behavior of electron and hole over AgPO@ZnInS follows a direct Z-scheme mechanism. Furthermore, the transformation pathway for degrading tetracycline was proposed by combining the Fukui index prediction with Mass Spectra identification of intermediates. This work presents in-depth sights into a regulated degradation pathway from theoretical prediction and practical identification based on innovative Z-scheme photocatalyst.
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http://dx.doi.org/10.1016/j.jcis.2020.10.140DOI Listing
March 2021

Gastrointestinal events with PARP inhibitors in cancer patients: A meta-analysis of phase II/III randomized controlled trials.

J Clin Pharm Ther 2020 Nov 2. Epub 2020 Nov 2.

College of Pharmacy, Southwest Minzu University, Chengdu, China.

What Is Known And Objective: PARP inhibitors are currently one of the most promising PARP targeted drugs for patients with certain types of cancer. Gastrointestinal (GI) events are common adverse events for all PARP inhibitors. We conducted this meta-analysis of randomized controlled trials (RCTs) to fully investigate the incidence and the relative risk of GI events in cancer patients receiving PARP inhibitors.

Study Design: Randomized controlled trials in cancer patients treated with PARP inhibitors were retrieved, and the systematic evaluation was conducted. Embase and PubMed/Medline were searched for articles published till July 2020.

Results: Twenty-nine RCTs and 9529 patients were included. The present meta-analysis suggests that the use of PARP inhibitors significantly increases the risk of developing all-grade nausea (RR, 1.46; 95% CI, 1.29-1.66; p < .00001), vomiting (RR, 1.39; 95% CI, 1.17-1.64; p = .0001), diarrhoea (RR, 1.14; 95% CI, 1.06-1.23; p = .0003) and decreased appetite (RR, 1.24; 95% CI, 1.14-1.36; p < .00001), but not for constipation. And the use of these agents significantly increased the risk of high-grade nausea (RR, 1.99; 95% CI, 1.44-2.74; p < .0001), vomiting (RR, 1.54; 95% CI, 1.11-2.14; p = .01) and decreased appetite (RR, 2.03; 95% CI, 1.22-3.40; p = .007), except for diarrhoea and constipation. Nausea was the most common GI event for these agents. Patients receiving veliparib were associated with a relatively lower risk of all-grade nausea and vomiting. Patients with ovarian cancer tend to have a higher risk of all-grade nausea and vomiting than those with non-ovarian cancer. The risk of all-grade nausea and vomiting tended to be higher when PARP inhibitors treatment was longer.

What Is New And Conclusion: PARP inhibitors were associated with a significant increased risk of GI events. Clinicians should be aware of these risks and perform regular monitoring.
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http://dx.doi.org/10.1111/jcpt.13300DOI Listing
November 2020

Ultrafast control of fractional orbital angular momentum of microlaser emissions.

Light Sci Appl 2020 21;9:179. Epub 2020 Oct 21.

Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104 USA.

On-chip integrated laser sources of structured light carrying fractional orbital angular momentum (FOAM) are highly desirable for the forefront development of optical communication and quantum information-processing technologies. While integrated vortex beam generators have been previously demonstrated in different optical settings, ultrafast control and sweep of FOAM light with low-power control, suitable for high-speed optical communication and computing, remains challenging. Here we demonstrate fast control of the FOAM from a vortex semiconductor microlaser based on fast transient mixing of integer laser vorticities induced by a control pulse. A continuous FOAM sweep between charge 0 and charge +2 is demonstrated in a 100 ps time window, with the ultimate speed limit being established by the carrier recombination time in the gain medium. Our results provide a new route to generating vortex microlasers carrying FOAM that are switchable at GHz frequencies by an ultrafast control pulse.
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http://dx.doi.org/10.1038/s41377-020-00415-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576132PMC
October 2020

Repeatable and Reprogrammable Shape Morphing from Photoresponsive Gold Nanorod/Liquid Crystal Elastomers.

Adv Mater 2020 Nov 12;32(46):e2004270. Epub 2020 Oct 12.

Department of Materials Science and Engineering, University of Pennsylvania, 3231 Walnut Street, Philadelphia, PA, 19104, USA.

Liquid crystal elastomers (LCEs) are of interest for applications such as soft robotics and shape-morphing devices. Among the different actuation mechanisms, light offers advantages such as spatial and local control of actuation via the photothermal effect. However, the unwanted aggregation of the light-absorbing nanoparticles in the LCE matrix will limit the photothermal response speed, actuation performance, and repeatability. Herein, a near-infrared-responsive LCE composite consisting of up to 0.20 wt% poly(ethylene glycol)-modified gold nanorods (AuNRs) without apparent aggregation is demonstrated. The high Young's modulus, 20.3 MPa, and excellent photothermal performance render repeated and fast actuation of the films (actuation within 5 s and recovery in 2 s) when exposed to 800 nm light at an average output power of ≈1.0 W cm , while maintaining a large actuation strain (56%). Further, it is shown that the same sheet of AuNR/LCE film (100 µm thick) can be morphed into different shapes simply by varying the motifs of the photomasks.
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http://dx.doi.org/10.1002/adma.202004270DOI Listing
November 2020

Stereoselective in vitro metabolism of cyproconazole in rat liver microsomes and identification of major metabolites.

Chemosphere 2021 Feb 1;264(Pt 2):128495. Epub 2020 Oct 1.

School of Chemistry, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou, 510006, PR China.

The vast usage of agrochemicals enhances food security globally but may pose challenge to understand the risk assessment to non-target organisms and human beings, and liver microsomes are responsible for metabolism of these agrochemicals in vivo. In this study, stereoselective metabolism of chiral triazole fungicide cyproconazole in rat liver microsomes has been investigated through chiral LC-MS/MS technique. The half-lives of four cyproconazole stereoisomers were different ranging from 95 to 187 min, and (2S, 3R)-cyproconazole preferentially metabolized in rat liver microsomes. In addition, the results from metabolism kinetic study indicated that rat liver microsomes showed the stronger potency to deplete (2S, 3R)-cyproconazole than the others. Then, homology modeling and molecular docking results revealed that the docking energy between (2S, 3R)-cyproconazole and the cytochrome P450 CYP3A1 (-7.46 kcal⋅mol) was higher than the others, meaning that (2S, 3R)-cyproconazole exhibited the strongest binding ability to this enzyme. Moreover, two main metabolites of cyproconazole coming from hydroxylation and dehydration were observed, and possible metabolic reactions of cyproconazole in rat liver microsomes were identified through using an LCQ ion trap mass spectrometer. This kind of systematic metabolic investigation of cyproconazole at chiral level would provide valuable information for ecological and human health risk assessment of chiral pesticides.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128495DOI Listing
February 2021

Dual-beam antiphase method to improve the WMS measurement limit in long-distance methane detection.

Appl Opt 2020 Sep;59(27):8217-8223

Wavelength modulation spectroscopy (WMS) with second harmonic detection is an extremely effective technique to detect gases in site applications. However, the significant levels of nonlinear effects in a system give rise to high background signals that either limit detection sensitivity or distort the harmonic signals. This paper outlines the theory of WMS-involved background signals and focuses on the elimination of undesirable effects in the background. A real-time, long-distance methane sensor using a tunable diode laser near 1653.7 nm is developed to continuously monitor methane by using a variable optical attenuator to suppress the background. Trace methane detection experiments verify that the minimum detection limit of the system can be increased by 47.5 times compared to the traditional WMS method.
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http://dx.doi.org/10.1364/AO.402774DOI Listing
September 2020

Distribution and sources of polycyclic aromatic hydrocarbons in the water column of Kongsfjorden, Arctic.

J Environ Sci (China) 2020 Nov 4;97:186-193. Epub 2020 Jun 4.

National Marine Environmental Monitoring Center, Dalian 116023, China; Hainan Tropical Ocean University, Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources, Ministry of Education, China. Electronic address:

Kongsfjorden is known for its characteristic multi-layer water mass formed by the convergence of freshwaters from nearby glaciers and rivers and saline water from the Atlantic and Arctic. The distribution of polycyclic aromatic hydrocarbons (PAHs) in the water column of Kongsfjorden was investigated and their potential sources were analyzed. The total concentrations of 16 PAHs in the surface seawater and river water were in the range of 33.4-79.8 ng/L (mean 48.5 ng/L) and 2.3-201.4 ng/L (mean 126.1 ng/L), respectively. Horizontally, PAHs were mainly concentrated around river estuaries and the glacier front in the dissolved phase. Vertically, the PAHs in the particulate phase followed surface-enrichment and depth-depletion patterns in most stations, with the maximum concentration found at 50 m depth in the central area of Kongsfjorden. The compositions of PAHs in seawater and rivers were similar, with two-ring and tricyclic PAHs comprising the majority of the dissolved and particulate phases. PAHs found in Kongsfjorden waters appeared to be derived from multiple sources such as petroleum and coal combustion. PAHs in the bay mouth of Kongsfjorden were mainly introduced by the West Spitsbergen Current and the Arctic waters, while in the inner bay, atmospheric deposition and local sources were the major contributors. The distribution of PAHs was mainly attributed to the suspended particulate distribution.
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http://dx.doi.org/10.1016/j.jes.2020.04.024DOI Listing
November 2020

Immunomagnetic separation of circulating tumor cells with microfluidic chips and their clinical applications.

Biomicrofluidics 2020 Jul 19;14(4):041502. Epub 2020 Aug 19.

School of Chemistry and Chemical Engineering, Anhui University of Technology, Maanshan 243002, China.

Circulating tumor cells (CTCs) are tumor cells detached from the original lesion and getting into the blood and lymphatic circulation systems. They potentially establish new tumors in remote areas, namely, metastasis. Isolation of CTCs and following biological molecular analysis facilitate investigating cancer and coming out treatment. Since CTCs carry important information on the primary tumor, they are vital in exploring the mechanism of cancer, metastasis, and diagnosis. However, CTCs are very difficult to separate due to their extreme heterogeneity and rarity in blood. Recently, advanced technologies, such as nanosurfaces, quantum dots, and Raman spectroscopy, have been integrated with microfluidic chips. These achievements enable the next generation isolation technologies and subsequent biological analysis of CTCs. In this review, we summarize CTCs' separation with microfluidic chips based on the principle of immunomagnetic isolation of CTCs. Fundamental insights, clinical applications, and potential future directions are discussed.
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http://dx.doi.org/10.1063/5.0005373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440929PMC
July 2020

Leveraging subject-specific musculoskeletal modeling to assess effect of anterior cruciate ligament retaining total knee arthroplasty during walking gait.

Proc Inst Mech Eng H 2020 Dec 3;234(12):1445-1456. Epub 2020 Aug 3.

Technology Implementation Research Center, Department of Orthopaedic Surgery, Massachusetts General Hospital, Boston, MA, USA.

Bi-cruciate retaining total knee arthroplasty has several potential advantages including improved anteroposterior knee stability compared to contemporary posterior cruciate-retaining total knee arthroplasty. However, few studies have explored whether there is significant differences of knee biomechanics following bi-cruciate retaining total knee arthroplasty compared to posterior cruciate-retaining total knee arthroplasty. In the present study, subject-specific lower extremity musculoskeletal multi-body dynamics models for bi-cruciate retaining, bi-cruciate retaining without anterior cruciate ligament, and posterior cruciate-retaining total knee arthroplasty were developed based on the musculoskeletal modeling framework using force-dependent kinematics method and validated against in vivo telemetric data. The experiment data of two subjects who underwent total knee arthroplasty were obtained for the SimTK "Grand Challenge Competition" repository, and integrated into the musculoskeletal model. Five walking gait trials for each subject were used as partial inputs for the model to predict the knee biomechanics for bi-cruciate retaining, bi-cruciate retaining without anterior cruciate ligament, and posterior cruciate-retaining total knee arthroplasty. The results revealed significantly greater range of anterior/posterior tibiofemoral translation, and significantly more posterior tibial location during the early phase of gait and more anterior tibial location during the late phase of gait were found in bi-cruciate retaining total knee arthroplasty without anterior cruciate ligament when compared to the bi-cruciate retaining total knee arthroplasty. No significant differences in tibiofemoral contact forces, rotations, translations, and ligament forces between bi-cruciate retaining and posterior cruciate-retaining total knee arthroplasty during normal walking gait, albeit slight differences in range of tibiofemoral internal/external rotation and anterior/posterior translation were observed. The present study revealed that anterior cruciate ligament retention has a positive effect on restoring normal knee kinematics in bi-cruciate retaining total knee arthroplasty. Preservation of anterior cruciate ligament in total knee arthroplasty and knee implant designs interplay each other and both contribute to restoring normal knee kinematics in different types of total knee arthroplasty. Further evaluation of more demanding activities and subject data from patients with bi-cruciate retaining and posterior cruciate-retaining total knee arthroplasty via musculoskeletal modeling may better highlight the role of the anterior cruciate ligament and its stabilizing influence.
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http://dx.doi.org/10.1177/0954411920947204DOI Listing
December 2020

Inhibition of cellular communication network factor 1 (CCN1)-driven senescence slows down cartilage inflammaging and osteoarthritis.

Bone 2020 10 3;139:115522. Epub 2020 Jul 3.

Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, People's Republic of China. Electronic address:

Objective: To explore the role of cellular communication network factor 1 (CCN1) in cartilage inflammaging and osteoarthritis (OA) pathogenesis in the isolated primary human chondrocytes in vitro, cartilage explants ex vivo, and a pre-clinical mice model.

Methods: Recombinant human CCN1 stimulation and small interfering RNA inhibition were conducted in human chondrocytes. The RNA was extracted to quantify catabolic targets and pro-inflammatory genes and the proteins were probed with specific antibodies. IL-1β and IL-6 were monitored by ELISA. IHC was performed to evaluate important hypertrophic hallmarks and catabolic markers. The effects of Tanshinone IIA on chondrocytes were investigated in both time-dependent and dose-dependent processes. Cartilage explants were cultured in growth medium and further treated with Tanshinone IIA. The intra-articular injection was performed in 13 months old C57BL/6J mice. Safranin O and fast green staining were performed to evaluate the histological change of cartilage followed by a semi-quantitative analysis using the OARSI scoring system.

Results: RNA and protein levels of CCN1 increased in an age-dependent manner compared to young donors. Increased CCN1 expression was also found in the damaged area compared to the non-lesion area which correlated with the advanced pathological change in human OA. The overexpression of CCN1 promoted chondrocytes senescence, while the down-regulation of CCN1 by small interfering RNA reduced CCN1 production and limited inflammation secretion suggesting that CCN1 was a possible novel target to intervene OA. Inhibition of CCN1 by using Tanshinone IIA could reduce SASP components in a dose- and time-dependent manner. Additionally, our data showed that Tanshinone IIA was able to preserve articular cartilage integrity, suppress CCN1 production, and inhibit SASP factors in human cartilage explants and in aged mice model.

Conclusion: This study showed that CCN1 signaling aggravated cartilage inflammaing and matrix degradation. Collectively, our findings showed new insight into repurposing Tanshinone IIA for slowing down OA advancement in human and mice by inhibiting the CCN1 axis.
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http://dx.doi.org/10.1016/j.bone.2020.115522DOI Listing
October 2020

Nanostructure and its effect on electrochemical properties of polyanionic LiCoSiO for lithium ion batteries.

Nanotechnology 2020 Jun 25;31(42):425602. Epub 2020 Jun 25.

Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, People's Republic of China. University of Chinese of Academy of Sciences, Beijing 100049, People's Republic of China.

This work reports a facile strategy to synthesize carbon-coated LiCoSiO/C particles with rich nanostructures by a two-step scheme starting with a low-temperature hydrothermal method. The size and morphology of particle aggregates can be regulated by the (OH) concentration and viscosity of the precursor solution, a mixture of ethylene glycol and deionized water, for the hydrothermal synthesis. In addition to the good electrical conductivity from the carbon coating, the size of the primary nanoparticles and the mesopore associated with the aggregations play an important role in improving the electrochemical properties of polyanionic LiCoSiO. The low-dimensional belt-like and sheet-like LiCoSiO/C nanomaterials present a higher reversible capacity 136.6 mAh g and 147 mAh g, respectively, in the first charging-discharging cycle between 2 V and 4.6 V. XRD, SEM/TEM, and EDS are used to characterize the crystalline structure and aggregation patterns. TGA and Raman spectra are employed to analyze the carbon coating on different morphologies. The analysis of electrochemical impedance spectroscopy highlights the critical role of the interface between the electrolyte and particles. This study provides insights into the rational design and synthesis of high-performance polyanionic cathodes including silicates.
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http://dx.doi.org/10.1088/1361-6528/aba02dDOI Listing
June 2020

Pd-Catalyzed Decarboxylative Cycloaddition of Vinylethylene Carbonates with Isothiocyanates.

J Org Chem 2020 Jul 28;85(14):8773-8779. Epub 2020 Jun 28.

Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry & Materials Science, Northwest University, 1 Xuefu Road, Xi'an 710127, P. R. China.

An efficient route for formal [3 + 2] cycloaddition of vinylethylene carbonates with isothiocyanates was developed for the synthesis of 1,3-oxazolidine-2-thione derivatives. The zwitterionic π-allyl palladium intermediates formed in situ by decarboxylation of VECs acted as the three-membered synthons. In this transformation, the C-N bond formation was selectively realized over the C-S bond formation.
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http://dx.doi.org/10.1021/acs.joc.0c00243DOI Listing
July 2020

Characterization of clinical enterococci isolates, focusing on the vancomycin-resistant enterococci in a tertiary hospital in China: based on the data from 2013 to 2018.

BMC Infect Dis 2020 May 19;20(1):356. Epub 2020 May 19.

Department of Clinical Laboratory, Jinling Hospital, Nanjing University School of Medicine, Nanjing University, 305# East Zhongshan Road, Qinhuai District, Nanjing, Jiangsu Province, 210008, P. R. China.

Background: Vancomycin-resistant Enterococcus spp. (VRE) have spread all over the world. The present study aims to investigate the species distribution, specimen type and susceptibilities of Enterococcal species collected from Nanjing Drum Tower Hospital from 2013 to 2018. Additionally, distribution of VRE and prevalence of van gene among VRE isolates were also analyzed.

Methods: The susceptibilities of 3913 Enterococcus isolates were retrospectively investigated. Among these strains, 60 VRE strains were further anazlyed in this study. The minimum inhibitory concentrations (MICs) of the VRE strains towards vancomycin, teicoplanin and linezolid were determined by E-test. Polymerase chain reaction (PCR) and DNA sequencing were used to investigate the prevalence of van genes among VRE. Furthermore, the sequence types (STs) of VRE strains were explored by multi-locus sequence typing (MLST).

Results: Among the 3913 enterococci isolates, Enterococcus faecalis (n = 1870, 47.8%) and Enterococcus faecium (1738, 44.4%) were the main isolates. These Enterococcus strains were mainly isolated from urine (n = 1673, 42.8%), followed by secretions (n = 583, 14.9%) and ascites (n = 554, 14.2%). VRE displayed a decreasing trend year by year. Molecular analysis revealed that 49 out of 60 VRE isolates carried vanA gene, 10 carried vanM, and 1 carried both vanA and vanM genes. Sixteen distinct STs were identified among the 58 VREM, with ST78 (n = 16), ST192 (n = 8) and ST570 (n = 7) being the most dominant ones.

Conclusions: E. faecalis and E. faecium were the major enterococci strains which are the main pathogens of urinary traction infections; vanA and vanM were the main determinants conferring resistance to vancomycin; ST78, ST192 and ST570 were the leading STs of VREM which displayed a decreasing trend of prevalence year by year.
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http://dx.doi.org/10.1186/s12879-020-05078-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285731PMC
May 2020

Occurrence and sources of polycyclic aromatic hydrocarbons in atmosphere and soil from 2013 to 2019 in the Fildes Peninsula, Antarctica.

Mar Pollut Bull 2020 Jul 15;156:111173. Epub 2020 May 15.

National Marine Environmental Monitoring Center, Dalian 116023, China.

Long-term monitoring is essential for revealing pollution trends, but relevant studies in the Antarctic remain limited. In this study, a seven-year continuous monitoring of polycyclic aromatic hydrocarbons (PAHs) in the atmosphere and soil was carried out at the Fildes Peninsula, Antarctica. Average concentrations of ΣPAHs were 7134.491 pg/m and 61.093 ng/g in air and soil, respectively. A declining trend was observed for ΣPAHs in air during the 2013-2019 summer, but this was not found in soil. Potential sources of PAHs in the Fildes Peninsula were identified by PMF modeling, correlation analysis, air mass back-trajectories and component analysis. The results showed that PAHs in the gas phase were more easily influenced by long-range atmospheric transport (LRAT) than in the particle phase. Moreover, temperature played a key role in the PAHs concentration in particle phase. Occurrence of ΣPAHs in soil was mainly attributed to local sources including fossil fuel combustion and spilling.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111173DOI Listing
July 2020

Photocurrent detection of the orbital angular momentum of light.

Science 2020 05;368(6492):763-767

Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA.

Applications that use the orbital angular momentum (OAM) of light show promise for increasing the bandwidth of optical communication networks. However, direct photocurrent detection of different OAM modes has not yet been demonstrated. Most studies of current responses to electromagnetic fields have focused on optical intensity-related effects, but phase information has been lost. In this study, we designed a photodetector based on tungsten ditelluride (WTe) with carefully fabricated electrode geometries to facilitate direct characterization of the topological charge of OAM of light. This orbital photogalvanic effect, driven by the helical phase gradient, is distinguished by a current winding around the optical beam axis with a magnitude proportional to its quantized OAM mode number. Our study provides a route to develop on-chip detection of optical OAM modes, which can enable the development of next-generation photonic circuits.
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http://dx.doi.org/10.1126/science.aba9192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545405PMC
May 2020

Tunable topological charge vortex microlaser.

Science 2020 05;368(6492):760-763

Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA.

The orbital angular momentum (OAM) intrinsically carried by vortex light beams holds a promise for multidimensional high-capacity data multiplexing, meeting the ever-increasing demands for information. Development of a dynamically tunable OAM light source is a critical step in the realization of OAM modulation and multiplexing. By harnessing the properties of total momentum conservation, spin-orbit interaction, and optical non-Hermitian symmetry breaking, we demonstrate an OAM-tunable vortex microlaser, providing chiral light states of variable topological charges at a single telecommunication wavelength. The scheme of the non-Hermitian-controlled chiral light emission at room temperature can be further scaled up for simultaneous multivortex emissions in a flexible manner. Our work provides a route for the development of the next generation of multidimensional OAM-spin-wavelength division multiplexing technology.
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http://dx.doi.org/10.1126/science.aba8996DOI Listing
May 2020

Synthesis of Indolizine Derivatives Triggered by the Oxidative Addition of Aroyl Chloride to Pd(0) Complex.

J Org Chem 2020 May 7;85(10):6392-6399. Epub 2020 May 7.

Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710127, P. R. China.

An efficient synthesis of indolizine derivatives from propargylic pyridines and aroyl chlorides was developed. The 5-endo-dig cyclization was initiated by the in situ formed acylpalladium species from the facile oxidative addition of aroyl chloride to Pd(0) complex. This transformation successfully occurred in the presence of an -nucleophilic moiety and acid chlorides, a good electrophilic partner, affording highly functionalized indolizines in good-to-excellent yields.
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http://dx.doi.org/10.1021/acs.joc.0c00161DOI Listing
May 2020

Publisher Correction: Evolutionary transcriptomics of metazoan biphasic life cycle supports a single intercalation origin of metazoan larvae.

Nat Ecol Evol 2020 05;4(5):766

MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41559-020-1208-4DOI Listing
May 2020

Evolutionary transcriptomics of metazoan biphasic life cycle supports a single intercalation origin of metazoan larvae.

Nat Ecol Evol 2020 05 16;4(5):725-736. Epub 2020 Mar 16.

MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.

The transient larva-bearing biphasic life cycle is the hallmark of many metazoan phyla, but how metazoan larvae originated remains a major enigma in animal evolution. There are two hypotheses for larval origin. The 'larva-first' hypothesis suggests that the first metazoans were similar to extant larvae, with later evolution of the adult-added biphasic life cycle; the 'adult-first' hypothesis suggests that the first metazoans were adult forms, with the biphasic life cycle arising later via larval intercalation. Here, we investigate the evolutionary origin of primary larvae by conducting ontogenetic transcriptome profiling for Mollusca-the largest marine phylum characterized by a trochophore larval stage and highly variable adult forms. We reveal that trochophore larvae exhibit rapid transcriptome evolution with extraordinary incorporation of novel genes (potentially contributing to adult shell evolution), and that cell signalling/communication genes (for example, caveolin and innexin) are probably crucial for larval evolution. Transcriptome age analysis of eight metazoan species reveals the wide presence of young larval transcriptomes in both trochozoans and other major metazoan lineages, therefore arguing against the prevailing larva-first hypothesis. Our findings support an adult-first evolutionary scenario with a single metazoan larval intercalation, and suggest that the first appearance of proto-larva probably occurred after the divergence of direct-developing Ctenophora from a metazoan ancestor.
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http://dx.doi.org/10.1038/s41559-020-1138-1DOI Listing
May 2020