Publications by authors named "Zhifeng Li"

137 Publications

Different Metabolites in the Roots, Seeds, and Leaves of and Their Role in Alleviating Oxidative Stress.

J Anal Methods Chem 2021 15;2021:6628880. Epub 2021 Apr 15.

State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang 330006, China.

In this study, we examined the metabolites from different parts of and their role in alleviating damage caused by oxidative stress. We used UHPLC-QTOF-MS to analyze the chemical components in the root, seed, and leaf extracts of . Two multivariate statistical analysis methods-namely, principal component analysis and partial least square discriminant analysis-were used to distinguish the samples obtained from different parts of the plant. Using univariate statistics, 130 different metabolites were screened out. Among these, the relative content of flavonoids and terpenoids was found to be highest in the leaves, the lignin and phenolic acid content was highest in the roots, and the amino acid and phenolic acid levels were highest in seeds. An MTT assay was used to test the anti-HO oxidative damage to PC12 cells in different parts of the sample. Lastly, using Pearson's correlation analysis, various metabolites from different parts of were correlated with their antioxidant effects from the corresponding parts. Fifty-two related different metabolites were found, of which 20 metabolites that were positively correlated to oxidative stress were present at a relatively higher level in the roots, whereas 32 metabolites that were negatively correlated were present at relatively higher levels in the seeds and leaves. The results of this study reveal the distribution characteristics and the antioxidant activity of different metabolites of and provide a reference for the rational development of its medicinal parts.
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http://dx.doi.org/10.1155/2021/6628880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064801PMC
April 2021

Transcriptomic and Metabolomic Profiling Reveals the Protective Effect of (Rupr. & Maxim.) Harms Combined With Blume on Cerebral Ischemia-Reperfusion Injury.

Front Pharmacol 2021 16;12:619076. Epub 2021 Apr 16.

State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, China.

The effects of current treatment strategies used in ischemic stroke are weakened by cerebral ischemia-reperfusion (CIR) injury. Suitable treatment regimens targeting CIR injury are still lacking. Two herbs, namely, (Rupr. & Maxim.) Harms (ASE) and Blume (GEB), have been used as traditional Chinese medicine and are indicated in the treatment of stroke and cerebrovascular diseases. However, there are no studies that report the effects of ASE combined with GEB in the treatment of CIR injury. In this study, we used the Zea Longa method to induce CIR injury in male Wistar rats. Results of the pharmacodynamic studies revealed that co-administration of ASE and GEB may improve neuronal injury and prevent neuronal apoptosis by reducing oxidative stress and inflammation, and also help prevent CIR injury. On the basis of our hypothesis, we combined the results from transcriptomic and metabonomic analyses and found that ASE and GEB could prevent CIR injury by targeting phenylalanine, pyrimidine, methionine, and sphingolipid metabolism. Therefore, our study provides the basis for the compatibility and efficacy of ASE and GEB.
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http://dx.doi.org/10.3389/fphar.2021.619076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085551PMC
April 2021

Epidemiological and clinical characteristics of severe fever with thrombocytopenia syndrome bunyavirus human-to-human transmission.

PLoS Negl Trop Dis 2021 Apr 30;15(4):e0009037. Epub 2021 Apr 30.

Jiangsu Provincial Center for Disease Control and Prevention (Jiangsu institution of Public health), Nanjing, China.

Background: Severe fever with thrombocytopenia syndrome (SFTS) was listed as one of the most severe infectious disease by world health organization in 2017. It can mostly be transmitted by tick bite, while human-to-human transmission has occurred on multiple occasions. This study aimed to explore the epidemiological and clinical characteristics and make risk analysis of SFTS human-to-human transmission.

Methods: Descriptive and spatial methods were employed to illustrate the epidemiological and clinical characteristics of SFTS human-to-human transmission. The risk of SFTS human-to-human transmission was accessed through secondary attack rate (SAR) and basic reproductive number (R0). Logistic regression analysis was used to identify the associated risk factors.

Results: A total of 27 clusters of SFTS human-to-human transmission were reported in China and South Korea during 1996-2019. It mainly occurred among elder people in May, June and October in central and eastern China. The secondary cases developed milder clinical manifestation and better outcome than the index cases. The incubation period was 10.0 days (IQR:8.0-12.0), SAR was 1.72%-55.00%, and the average R0 to be 0.13 (95%CI:0.11-0.16). Being blood relatives of the index case, direct blood/bloody secretion contact and bloody droplet contact had more risk of infection (OR = 6.35(95%CI:3.26-12.37), 38.01 (95%CI,19.73-73.23), 2.27 (95%CI,1.01-5.19)).

Conclusions: SFTS human-to-human transmission in China and South Korea during 1996-2019 had obvious spatio-temporal distinction. Ongoing assessment of this transmission risk is crucial for public health authorities though it continues to be low now.
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http://dx.doi.org/10.1371/journal.pntd.0009037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087050PMC
April 2021

Dynamically tunable ultra-narrowband perfect absorbers for the visible-to-infrared range based on a microcavity integrated graphene pair.

Opt Lett 2021 May;46(9):2236-2239

Dynamically tunable ultra-narrowband perfect absorbers are important to next-generation active photonic devices. A composite structure of a graphene pair and a microcavity with Bragg mirrors are proposed for this purpose. Based on the electrically controllable doping of graphene and critical coupling of the incident light, the microcavity-graphene composite structure achieves peak absorptance higher than 99.5%, a relative peak width ($\Delta \lambda /{\lambda _0}$) smaller than 1.1%, and a modulation depth larger than 92.0% throughout the visible-to-mid-infrared range, surpassing other structures in comprehensive performance. By changing the number of the dielectric pairs in the Bragg mirrors, the device can become an amplitude or a spectral modulator. The results are based on the optical constants from experiment data, including the surface conductivity of graphene with relatively low mobility, so they are more useful in practical situations.
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http://dx.doi.org/10.1364/OL.423674DOI Listing
May 2021

Fludarabine Inhibits Infection of Zika Virus, SFTS Phlebovirus, and Enterovirus A71.

Viruses 2021 04 27;13(5). Epub 2021 Apr 27.

Jiangsu Key Laboratory of Molecular Medicine, Medical School, Nanjing University, Nanjing 210093, China.

Viral infections are one of the leading causes in human mortality and disease. Broad-spectrum antiviral drugs are a powerful weapon against new and re-emerging viruses. However, viral resistance to existing broad-spectrum antivirals remains a challenge, which demands development of new broad-spectrum therapeutics. In this report, we showed that fludarabine, a fluorinated purine analogue, effectively inhibited infection of RNA viruses, including Zika virus, Severe fever with thrombocytopenia syndrome virus, and Enterovirus A71, with all IC values below 1 μM in Vero, BHK21, U251 MG, and HMC3 cells. We observed that fludarabine has shown cytotoxicity to these cells only at high doses indicating it could be safe for future clinical use if approved. In conclusion, this study suggests that fludarabine could be developed as a potential broad-spectrum anti-RNA virus therapeutic agent.
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http://dx.doi.org/10.3390/v13050774DOI Listing
April 2021

Disulfiram/copper shows potent cytotoxic effects on myelodysplastic syndromes via inducing Bip-mediated apoptosis and suppressing autophagy.

Eur J Pharmacol 2021 Apr 16;902:174107. Epub 2021 Apr 16.

Department of Hematology, The First Affiliated Hospital of Xiamen University and Institute of Hematology, School of Medicine, Xiamen University, Xiamen, 361003, China; Key Laboratory of Xiamen for Diagnosis and Treatment of Hematological Malignancy, Xiamen, 361003, China. Electronic address:

Patients with myelodysplastic syndromes (MDS) who resist or fail to respond to hypomethylating agents (HMAs) show very poor outcomes and have no effective treatment strategies. Therefore, new therapeutic approaches are urgently needed for MDS patients harboring adverse prognostic factors. Repurposing disulfiram (DSF), an alcohol-abuse drug, with or without Copper (Cu) has attracted considerable attentions as a candidate anti-tumor therapy in diverse malignancies. However, the effect of DSF in the presence or absence of Cu on MDS has not been reported yet. In this study, we found that monotherapy with DSF showed mild cytotoxic effects on MDS preclinical models. However, the anti-tumor activity of DSF was significantly enhanced in the presence of Cu in MDS in vitro and in vivo with minimal safety profiles. DSF/Cu combination blocked MDS cell cycle progression at the G0/G1 phase, accompanied by reduction of the S phase. Accordingly, co-treatment with DSF and Cu downregulated the expression of Cyclin D1 and Cyclin A2, whereas this combination upregulated the level of P21 and P27. Mechanistically, the anti-MDS effectiveness of DSF/Cu was potentially associated with activation of the ER stress-related Bip pathway and inactivation of the Akt pathway. In addition, inhibition of autophagy process also contributed to the cytotoxicity of DSF/Cu in MDS cells. In conclusion, these findings provide impressive evidence that the DSF/Cu complex shows potent anti-tumor efficacies on MDS preclinical models, representing a potential alternative therapy for MDS patients and warranting further investigation in clinical contexts.
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http://dx.doi.org/10.1016/j.ejphar.2021.174107DOI Listing
April 2021

Combined role of polarization matching and critical coupling in enhanced absorption of 2D materials based on metamaterials.

Opt Express 2021 Mar;29(6):9269-9282

Since 2D materials are typically much more efficient to absorb in-plane polarized light than out-of-plane polarized light, keeping the light polarization in-plane at the 2D material is revealed to be a crucial factor other than critical coupling in light absorption enhancement in a 2D material integrated with a light coupling structure. When the composite of a metal-insulator-metal structure and a 2D material changes from the magnetic resonator form to the metasurface Salisbury screen one, the field polarization at the 2D material changes from a mainly out-of-plane status to a mainly in-plane status. As a result, for graphene, the absorptance enhancement is increased by 1.6 to 4.2 times, the bandwidth enlarged by 3.6 to 6.4 times, and the metal loss suppressed by 7.4 to 24 times in the mid- to far-infrared range, leading to the absorptance of graphene approaching 90% in the mid-infrared regime and 100% in the THz regime. For monolayer black phosphorus, the absorptance enhancement at the wavelength of 3.5 µm is increased by 5.4 times, and the bandwidth enlarged by 1.8 times. For monolayer MoS, the averaged absorptance in the visible-near infrared range is enhanced by 4.4 times from 15.5% to 68.1%.
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http://dx.doi.org/10.1364/OE.419028DOI Listing
March 2021

Secondary myeloma in patients with chronic lymphoblastic leukemia: A case report and analysis of data from SEER database.

Curr Probl Cancer 2021 Mar 6:100728. Epub 2021 Mar 6.

Department of Hematology, the First Affiliated Hospital of Xiamen University, Xiamen, People's Republic of China. Electronic address:

Both chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) are common hematological malignancies originating from mature B cells of different differentiation stage. However, it is quite rare that MM could develop after CLL diagnosed. We reported a 66-year-old female progressed forward myeloma 3 years after she was diagnosed as CLL and conducted an analysis to investigate the epidemiology and clinical features among these patients based on the Surveillance, Epidemiology, and End Results (SEER) database. Our data demonstrated that CLL patients were 19% less likely to develop myeloma than general U.S. population (standardized incidence ratio 0.81; 95% confidence interval 0.62-1.03), although without statistical difference. The median overall survival from CLL diagnosed was 90 (58.1-121.9) months, which was the same as general CLL patients according to historical data. But the outcomes of secondary MM was much poorer than general MM patients. Age and gender were independent factors that impact the survival among these patients.
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http://dx.doi.org/10.1016/j.currproblcancer.2021.100728DOI Listing
March 2021

Studies on effect of Tongfengxiaofang in HUM model mice using a UPLC-ESI-Q-TOF/MS metabolomic approach.

Biomed Chromatogr 2021 Mar 22:e5118. Epub 2021 Mar 22.

State Key Laboratory of Innovative Drug and Efficient Energy-saving Pharmaceutical Equipment, Nanchang, China.

Hyperuricemia (HUM) is a major risk factor for the development of gout. The traditional Chinese medicine (TCM) complex prescription Tongfengxiaofang (TFXF) is composed of a variety of TCMs. To study the therapeutic effect of TFXF on HUM mice and the mechanisms by which it exerts a therapeutic effect, the biochemical indices were measured and qPCR technique was used. In addition, plasma metabolomics analysis was carried out based on UPLC-Q-TOF/MS to evaluate the characteristics of the metabolic spectrum changes. TFXF significantly downregulated the contents of uric acid, urea nitrogen and creatinine in serum and the concentration of xanthine oxidase in liver of HUM mice. In addition, TFXF significantly inhibited the overexpression of uric acid transporter 1 and glucose transporter 9 and upregulated the expression of organic anion transporter 1 in the kidney. A total of 152 metabolites were identified and 11 key biomarkers were further selected from these pathways to understand the mechanism of TFXF on the arginine biosynthesis, galactose metabolism, pyrimidine metabolism, glycerophospholipid metabolism, tryptophan metabolism and the citrate cycle (TCA cycle). The results of this confirmed the effect of TFXF on HUM and revealed the metabolic activity mechanism.
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http://dx.doi.org/10.1002/bmc.5118DOI Listing
March 2021

ORF8 contributes to cytokine storm during SARS-CoV-2 infection by activating IL-17 pathway.

iScience 2021 Apr 9;24(4):102293. Epub 2021 Mar 9.

School of Life Sciences, Chongqing University, Chongqing 401331, China.

Recently, COVID-19 caused by the novel coronavirus SARS-CoV-2 has brought great challenges to the world. More and more studies have shown that patients with severe COVID-19 may suffer from cytokine storm syndrome; however, there are few studies on its pathogenesis. Here we demonstrated that SARS-CoV-2 coding protein open reading frame 8 (ORF8) acted as a contributing factor to cytokine storm during COVID-19 infection. ORF8 could activate IL-17 signaling pathway and promote the expression of pro-inflammatory factors. Moreover, we demonstrated that treatment of IL17RA antibody protected mice from ORF8-induced inflammation. Our findings are helpful to understand the pathogenesis of cytokine storm caused by SARS-CoV-2 and provide a potential target for the development of COVID-19 therapeutic drugs.
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http://dx.doi.org/10.1016/j.isci.2021.102293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7942160PMC
April 2021

Microscopic structure and dynamics study of granular segregation mechanism by cyclic shear.

Sci Adv 2021 Feb 17;7(8). Epub 2021 Feb 17.

School of Physics and Astronomy, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240, China.

Granular mixtures with size difference can segregate upon shaking or shear. However, the quantitative study of this process remains difficult because it can be influenced by many mechanisms. Conflicting results on similar experimental systems are frequently obtained when the experimental conditions are not well controlled, which is mainly due to the fact that many mechanisms can be at work simultaneously. Moreover, it is often that macroscopic or empirical measures, which lack microscopic physical bases, are used to explain the experimental findings and therefore cannot provide an accurate and complete depiction of the overall process. Here, we carry out a detailed and systematic microscopic structure and dynamics study of a cyclically sheared granular system with rigorously controlled experimental conditions. We find that both convection and arching effect play important roles in the segregation process in our system, and we can quantitatively identify their respective contributions.
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http://dx.doi.org/10.1126/sciadv.abe8737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888927PMC
February 2021

Identification of a novel LRRC4C-ALK fusion in lung adenocarcinoma and outcome of the response to anaplastic lymphoma kinase inhibitors.

J Gene Med 2021 Mar 8;23(3):e3316. Epub 2021 Feb 8.

Medical Oncology, Cancer Hospital of Shantou University Medical College, Shantou, China.

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http://dx.doi.org/10.1002/jgm.3316DOI Listing
March 2021

Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF-B Signaling Pathway.

Evid Based Complement Alternat Med 2021 6;2021:8868527. Epub 2021 Jan 6.

State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang 330006, China.

Gouty arthritis is characterized by the deposition of monosodium urate (MSU) within synovial joints and tissues due to increased urate concentrations. Here, we elucidated the role of the natural compound cichoric acid (CA) on the MSU crystal-stimulated inflammatory response. The THP-1-derived macrophages (THP-Ms) were pretreated with CA and then stimulated with MSU suspensions. The protein levels of p65 and IB, the activation of the NF-B signaling pathway by measuring the expression of its downstream inflammatory cytokines, and the activity of NLRP3 inflammasome were measured by western blotting and ELISA. CA treatment markedly inhibited the degradation of IB and the activation of NF-B signaling pathway and reduced the levels of its downstream inflammatory genes such as IL-1, TNF-, COX-2, and PGE2 in the MSU-stimulated THP-M cells. Therefore, we infer that CA effectively alleviated MSU-induced inflammation by suppressing the degradation of IB, thereby reducing the activation of the NF-B signaling pathway and the NLRP3 inflammasome. These results suggest that CA could be a novel therapeutic strategy in averting acute episodes of gout.
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http://dx.doi.org/10.1155/2021/8868527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7808822PMC
January 2021

Appearance of an ALK mutation conferring resistance to crizotinib in non-small cell lung cancer harboring oncogenic ROS1 fusion.

Lung Cancer 2021 03 8;153:174-175. Epub 2021 Jan 8.

Medical Oncology, Cancer Hospital of Shantou University Medical College, Shantou, China. Electronic address:

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http://dx.doi.org/10.1016/j.lungcan.2021.01.001DOI Listing
March 2021

Visible-light-promoted selective -alkylation of 2-pyridones with α-aryldiazoacetates.

Org Biomol Chem 2021 01;19(2):394-398

College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.

A visible-light-promoted O-H insertion reaction between 2-pyridones and α-aryldiazoacetates has been developed. Upon visible light irradiation, the reaction proceeds smoothly under mild and catalyst-free conditions. A wide scope of 2-pyridones and α-aryldiazoacetates are well tolerated, and various O-alkylated 2-pyridones are obtained with perfect selectivity and good functional group tolerance. A photoinduced radical process is probably responsible for the excellent selectivity.
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http://dx.doi.org/10.1039/d0ob02350gDOI Listing
January 2021

Learning Spatial Attention for Face Super-Resolution.

IEEE Trans Image Process 2021 21;30:1219-1231. Epub 2020 Dec 21.

General image super-resolution techniques have difficulties in recovering detailed face structures when applying to low resolution face images. Recent deep learning based methods tailored for face images have achieved improved performance by jointly trained with additional task such as face parsing and landmark prediction. However, multi-task learning requires extra manually labeled data. Besides, most of the existing works can only generate relatively low resolution face images (e.g., 128×128 ), and their applications are therefore limited. In this paper, we introduce a novel SPatial Attention Residual Network (SPARNet) built on our newly proposed Face Attention Units (FAUs) for face super-resolution. Specifically, we introduce a spatial attention mechanism to the vanilla residual blocks. This enables the convolutional layers to adaptively bootstrap features related to the key face structures and pay less attention to those less feature-rich regions. This makes the training more effective and efficient as the key face structures only account for a very small portion of the face image. Visualization of the attention maps shows that our spatial attention network can capture the key face structures well even for very low resolution faces (e.g., 16×16 ). Quantitative comparisons on various kinds of metrics (including PSNR, SSIM, identity similarity, and landmark detection) demonstrate the superiority of our method over current state-of-the-arts. We further extend SPARNet with multi-scale discriminators, named as SPARNetHD, to produce high resolution results (i.e., 512×512 ). We show that SPARNetHD trained with synthetic data can not only produce high quality and high resolution outputs for synthetically degraded face images, but also show good generalization ability to real world low quality face images. Codes are available at https://github.com/chaofengc/Face-SPARNet.
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http://dx.doi.org/10.1109/TIP.2020.3043093DOI Listing
December 2020

The role of carbon nanoparticle in lymph node detection and parathyroid gland protection during thyroidectomy for non-anaplastic thyroid carcinoma- a meta-analysis.

PLoS One 2020 10;15(11):e0223627. Epub 2020 Nov 10.

Department of Head and Neck Surgery, Cancer Hospital, Shantou University Medical College, Shantou, China.

Objective: To assess the efficiency of the carbon nanoparticles (CNs) in lymph node identification and parathyroid gland (PG) protection during thyroidectomy for non-anaplastic thyroid carcinoma (N-ATC).

Methods: A systematic literature search for relevant literatures published up to December 2018 in PubMed, EMBASE, Web of Science and Cochrane Library was performed. Both English and Chinese literatures were retrieved and analyzed. Randomized controlled trials or nonrandomized controlled trials comparing the use of CNs with the use of methylene blue or a blank control in patients undergoing thyroidectomy for N-ATC were enrolled in this study. The primary outcomes included the number of lymph nodes harvested, the rate of lymph nodes involved, and the rates of accidental parathyroidectomy, hypoparathyroidism, and hypocalcemia. Weighted mean differences (WMDs), odds ratios (ORs) and risk differences (RDs) were calculated for the dichotomous outcome variables. Between study heterogeneity was tested using the Q tests and the I2 statistics. All analyses were performed using Review Manager (version 5.3.5).

Results: 25 studies comprising 3266 patients were included in this analysis. The total number of lymph nodes harvested in the CNs groups was significantly higher than that in the control groups (WMD, 2.36; 95% CI, 1.40 to 3.32; P <0.01). Administrating CNs was associated with a lower incidence of accidental PG removal (OR = 0.28, 95% CI = 0.21 to 0.37, P<0.01) and lower rates of both postoperative transient hypoparathyroidism (OR = 0.46, 95% CI = 0.33 to 0.64, P <0.01) and transient hypocalcemia (OR = 0.46, 95% CI = 0.33 to 0.65, P <0.01). No significant difference was found concerning lymph node metastatic rates between CNs group and control group. Subgroup analysis indicated that the application of CNs in reoperation thyroidectomy reduced both the rate of transient hypoparathyroidism (OR = 0.21, 95% CI = 0.06 to 0.75, P = 0.02) and the possibility of accidental PGs removal (OR = 0.21, 95% CI = 0.07 to 0.62, P = 0.004, P<0.05).

Conclusions: The application of CNs in thyroidectomy for N-ATC results in higher number of lymph node harvested and better PG protection during both initial and reoperation thyroidectomy.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0223627PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7654818PMC
December 2020

PTPN14 aggravates inflammation through promoting proteasomal degradation of SOCS7 in acute liver failure.

Cell Death Dis 2020 09 25;11(9):803. Epub 2020 Sep 25.

School of Life Sciences, Chongqing University, 401331, Chongqing, China.

Acute liver failure (ALF) is a rare but life-threatening systemic disorder. The innate immune regulation has an important role in this process; however, the specific mechanisms are not completely clear. Using the LPS + D-GalN-induced ALF mouse model, we found that the survival rate of PTPN14-deficient mice was higher than that of the control group, while the release of inflammatory factors was significantly lower. We further showed that PTPN14 interacted with SOCS7, and promoted the degradation of SOCS7 through ubiquitination at K11 and K48, thereby reducing the protein level of SOCS7 and weakening the inhibitory effects on inflammatory factors. More importantly, SOCS7 blocked the NF-κB signaling pathway by preventing the activity of the IKK complex, and then reduced the expression of downstream inflammatory factors. In this study, we firstly reported the inhibitory effect of SOCS7 on the NF-κB pathway in the ALF mouse model and elucidated the mechanism of PTPN14-SOCS7-NF-κB axis in the regulation of inflammation. These results provide new insights into the clinical treatment of ALF.
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http://dx.doi.org/10.1038/s41419-020-03014-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7519157PMC
September 2020

Mid-infrared polarization-controlled broadband achromatic metadevice.

Sci Adv 2020 Sep 11;6(37). Epub 2020 Sep 11.

National Laboratory for Infrared Physics, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, 500 Yu-Tian Road, Shanghai 200083, China.

Metasurfaces provide a compact, flexible, and efficient platform to manipulate the electromagnetic waves. However, chromatic aberration imposes severe restrictions on their applications in broadband polarization control. Here, we propose a broadband achromatic methodology to implement polarization-controlled multifunctional metadevices in mid-wavelength infrared with birefringent meta-atoms. We demonstrate the generation of polarization-controlled and achromatically on-axis focused optical vortex beams with diffraction-limited focal spots and switchable topological charge ( = 0 and = 2). Besides, we further implement broadband achromatic polarization beamsplitter with high polarization isolation (extinction ratio up to 21). The adoption of all-silicon configuration not only facilitates the integration with CMOS technology but also endows the polarization multiplexing meta-atoms with broad phase dispersion coverage, ensuring the large size and high performance of the metadevices. Compared with the state-of-the-art chromatic aberration-restricted polarization-controlled metadevices, our work represents a substantial advance and a step toward practical applications.
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http://dx.doi.org/10.1126/sciadv.abc0711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7486104PMC
September 2020

[Comparison of four scoring systems for predicting ICU mortality in patients with sepsis].

Nan Fang Yi Ke Da Xue Xue Bao 2020 Apr;40(4):513-518

Department of Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.

Objective: To evaluate the value of Sequential Organ Failure Assessment (SOFA), Simplified Acute Physiology Score Ⅱ (SAPS-Ⅱ), Oxford Acute Severity of Illness Score (OASIS) and Logistic Organ Dysfunction System (LODS) scoring systems for predicting ICU mortality in patients with sepsis.

Methods: We collected the data of a total of 2470 cases of sepsis recorded in the MIMIC-III database from 2001 to 2012 and retrieved the scores of SOFA, SAPS-Ⅱ, OASIS and LODS of the patients within the first day of ICU admission. We compared with the score between the survivors and the non-survivors and analyzed the differences in the area under the ROC curve (AUC) of the 4 scoring systems. Binomial logistic regression was performed to compare the predictive value of the 4 scoring systems for ICU mortality of the patients.

Results: In the 2470 patients with sepsis, 1966 (79.6%) survived and 504 (20.4%) died in the ICU. Compared with the survivors, the non-survivors had a significantly older mean age, higher proportion of patients receiving mechanical ventilation, and higher initial lactate level, creatinine, urea nitrogen, SOFA score, SAPS-Ⅱ score, OASIS score and LODS score ( < 0.05) but with significantly lower body weight and platelet counts ( < 0.05). The AUCs of the SOFA score, SAPS-Ⅱ score, OASIS score, and LODS score were 0.729 ( < 0.001), 0.768 ( < 0.001), 0.757 ( < 0.001), and 0.739 ( < 0.001), respectively. The AUC of SAPS-Ⅱ score was significantly higher than those of SOFA score (=3.679, < 0.001) and LODS score (=3.698, < 0.001) but was comparable with that of OASIS score (=1.102, =0.271); the AUC of OASIS score was significantly higher than that of LODS score (=2.172, =0.030) and comparable with that of SOFA score (=1.709, =0.088). For predicting ICU mortality in patients without septic shock, the AUC of SAPS-Ⅱ score was 0.769 (0.743-0.793), the highest among the 4 scoring systems; in patients with septic shock, the AUCs SAPS-Ⅱ score and OASIS score, 0.768 (0.745-0.791) and 0.762 (0.738-0.785), respectively, were significantly higher than those of the other two scoring systems. Binomial logistic regression showed the corrected SOFA, SAPS-Ⅱ, and OASIS scores, but not LODS scores, were significantly correlated with ICU mortality in patients with sepsis, and their ORs were 1.08 (95% CI: 1.03-1.14, =0.001), 1.04 (95% CI: 1.02-1.05, < 0.001), 1.04 (95% CI: 1.01-1.06, =0.001), 0.96 (95% CI: 0.89-1.04, =0.350), respectively.

Conclusions: The scores of SOFA, SAPS-Ⅱ, OASIS, and LODS can predict ICU mortality in patients with sepsis, but SAPS-Ⅱ and OASIS scores have better predictive value than SOFA and LODS scores.
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http://dx.doi.org/10.12122/j.issn.1673-4254.2020.04.10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225101PMC
April 2020

Clinical Course of 195 Critically Ill COVID-19 Patients: A Retrospective Multicenter Study.

Shock 2020 11;54(5):644-651

Department of Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.

Introduction: Coronavirus disease-2019 (COVID-19) outbreak has spread around the world. However, the dynamic course of critically ill COVID-19 has not been described thoroughly.

Patients And Methods: We retrospectively analyzed 195 critically ill COVID-19 patients in Hubei province, China, between January 5, 2020 and April 3, 2020. Epidemiologic data, clinical features, treatments, and outcomes were collected and analyzed.

Results: Most critically ill patients were older with higher Acute Physiology and Chronic Health Evaluation II scores. After critical illness onset, a total of 181 (92.8%) patients received ventilation support, of which 84 (43.1%) received noninvasive and 97 (49.7%) received invasive mechanic ventilation (IMV). Among the 97 patients with IMV, 28 (28.9%) received prone ventilation, 57 (58.8%) received neuromuscular blocked therapy, and 22 (11.3%) received tracheostomy due to prolonged ventilator use. Early hypoxemia, subsequent hypercapnia, pulmonary hypertension, and finally pulmonary fibrosis were notable in the clinical course of acute respiratory distress syndrome (ARDS). Eighty-nine (45.6%) patients presented with shock. Acute kidney injury (29.7%) and secondary infection (28.2%) were also notable. The overall mortality of critically ill patients at day 28 was 42.1%. Intensive care unit (ICU) mortality was around 33%, as 16 patients died prior to ICU admission. A low PaO2/FiO2 ratio was an independent risk factor for death. High viral load was observed in most non-survivors.

Conclusion: ARDS and shock were notable in the critical illness of COVID-19. Ventilation support and hemodynamic support were the cornerstones for critical care. High viral load was associated with death of critically ill COVID-19 patients.
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http://dx.doi.org/10.1097/SHK.0000000000001629DOI Listing
November 2020

Graphitic carbon nitride-based materials in activating persulfate for aqueous organic pollutants degradation: A review on materials design and mechanisms.

Chemosphere 2021 Jan 6;262:127675. Epub 2020 Aug 6.

College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, China; Research Centre for Resource and Environmental, Beijing, 100029, China. Electronic address:

With the increasingly serious water environment problem, the persulfate-based advanced oxidation process (PS-AOP) has attracted considerable attention in water pollution treatment. To date, graphitic carbon nitride (g-CN) has been greatly favored by researchers in activating PS for its capability and unique superiorities. Though g-CN-based PS-AOP exhibits huge development prospects in removing organic pollutants, the review about its research progress has not been reported. Herein, this paper reviews the modification of g-CN on the basis of its applications and properties for PS activation systematically. The activation mechanisms of g-CN-based modified materials are analyzed in detail, and the main formation pathways of radicals and non-radicals and their interaction mechanism with pollutants are thoroughly summarized. Finally, the existing challenges and future development directions of the PS-AOP driven by g-CN-based materials are critically discussed. The key purpose is to provide a reference for promoting the further popularization of this novel and efficient cooperative AOP in water purification industries, as well as multidisciplinary inspirations for g-CN-involved fields.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127675DOI Listing
January 2021

Pharmacokinetic and Lipidomic Assessment of the In Vivo Effects of Parishin A-Isorhynchophylline in Rat Migraine Models.

J Anal Methods Chem 2020 6;2020:9101598. Epub 2020 Jul 6.

Jiangxi University of Traditional Chinese Medicine, Nanchang, China.

Migraine is a chronic brain disease that leads to periodic neurological attacks. Parishin A and isorhynchophylline (PI) is the active monomer component extracted from the traditional antimigraine Chinese medicinal combination of Gastrodia and Uncaria, respectively. In this study, using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) technology, we performed pharmacokinetic and lipidomic study on migraine model rats after administration of PI. For the detection of the compounds in plasma, AB Sciex Triple Quad™ 4500 was applied for quantitative analysis, and the COSMOSIL C column (2.1 × 100 mm, 2.6 m) was used for separation. Isorhynchophylline (ISO: / 384.8-241.2) and its main metabolite rhynchophylline (RHY: / 384.8-160.2) were simultaneously detected under positive ion modes. Besides, parishin A (PA: / 995.1-726.9) and its main metabolite gastrodin (GAS: / 331.1-123.0) were simultaneously detected with negative ion modes. For the analysis of endogenous lipid components, Dionex Ultimate 3000 (UHPLC) Thermo Orbitrap Elite was applied for the detection, and the Waters UPLCRBEH C column (1.7 m 100  2.1 mm) was used for separation. Chloroform/methanol (2 : 1,  : ) was used for extraction. The results demonstrated that PI exists significant difference in metabolism between single- and coadministration and can regulate lipid levels associated with migraine.
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http://dx.doi.org/10.1155/2020/9101598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7362284PMC
July 2020

Low-dose triptolide enhances antitumor effect of JQ1 on acute myeloid leukemia through inhibiting RNA polymerase II in vitro and in vivo.

Mol Carcinog 2020 09 21;59(9):1076-1087. Epub 2020 Jul 21.

Department of Hematology, The First Affiliated Hospital of Xiamen University and Institute of Hematology, School of Medicine, Xiamen University, Xiamen, China.

The bromodomain and extra-terminal (BET) domain inhibitor JQ1 exerts potent anticancer activity in various cancer cells. However, the resistance to BET inhibitors in leukemia stem cells limits its implication in acute myeloid leukemia (AML). High concentration of triptolide (TPL) presents anticancer activities but with adverse effects. Here, we investigated whether the combination of low-dose TPL with JQ1 could help to circumvent the dilemma of drug resistance and side effect in treating AML. AML cell lines, primary cells from 10 AML patients with different status, as well as AML mice model were subjected to different treatments and apoptotic related protein expression were evaluated. Data showed that low-dose TPL combined with JQ1 effectively killed AML cell lines and primary cells from AML patients without exerting significantly greater lethal activity against normal cells. Mechanism study revealed that low-dose TPL combined with JQ1 triggered reactive oxygen species production and induced mitochondrial-mediated apoptosis in AML cells, in which the inhibition of RNA polymerase II to downregulate c-Myc was mainly responsible for the enhanced activity of TPL in combination with JQ1. In vivo study presented that cotreatment with low-dose TPL and JQ1 significantly reduced tumor burden of the NOD/SCID mice engrafted with MOLM-13 cells. In conclusion, low-dose TPL enhanced the antitumor effect of JQ1 on AML without increasing the side effects, supporting a potential option for AML treatment.
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http://dx.doi.org/10.1002/mc.23238DOI Listing
September 2020

Absorption enhancement in all-semiconductor plasmonic cavity integrated THz quantum well infrared photodetectors.

Opt Express 2020 May;28(11):16427-16438

The light coupling properties of all-semiconductor plasmonic cavity integrated THz quantum well infrared photodetectors were studied for absorption enhancement of the quantum wells. The all-semiconductor plasmonic cavity is constructed by heavily doped GaAs with a plasmonic behavior in the THz regime. The plasmonic behavior of GaAs was thoroughly studied by taking into account the carrier density dependent effective mass of electrons. An optimal doping level for GaAs to be the most metallic is selected since the plasma frequency of the doped GaAs varies nonmonotonically with the carrier density. By tuning the absorption competition between the quantum wells and the doped GaAs meanwhile keeping the system at a critical coupling status, the absorptance of the quantum wells is prominently enhanced by 13.2 times compared to that in a standard device. The all-semiconductor plasmonic cavity integrated quantum well photodetectors can be polarization sensitive (polarization extinction ratio > 900) when the plasmonic cavity is shaped into an anisotropic form. The good tolerance of the incident angle is favored for wide-field infrared detection. The GaAs plasmonic cavities are demonstrated to be effective when integrated at a pixel level, indicating a good compatibility with focal plane arrays.
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http://dx.doi.org/10.1364/OE.392230DOI Listing
May 2020

Shifts in the Bacterial Population and Ecosystem Functions in Response to Vegetation in the Yellow River Delta Wetlands.

mSystems 2020 Jun 9;5(3). Epub 2020 Jun 9.

State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao, People's Republic of China

Vegetation represents probably the most crucial step for the ecosystem functions of wetlands, but it is unclear how microbial populations and functions shift along with vegetation. In this study, we found that the richness and diversity of soil bacteria increased with vegetation levels and that the community composition was distinctly shifted from bare to vegetative places. The bare land displayed an extremely high abundance of as a monospecies genus, while a genus was predominant as multiple species in all the vegetative wetlands, suggesting their important ecosystem functions and potential mechanisms. Expression of the genes related to photosynthesis was enriched exclusively in bare land. Genes involved in biological organic carbon metabolism and the cycling of main elements (C, N, S, and P) were highly expressed in vegetative wetlands and were mostly included in the metagenome-assembled genome (MAG) of Some compounds identified from soil metabolomic results also corresponded to pathways involving these key active genes. is thus responsible for the carbon sink in early infertile wetlands, and plays a crucial role in ecosystem functions in vegetative wetlands. Our results highlight that the soil microbial populations execute ecosystem functions for wetlands and that vegetation is the determinant for the population and functional shifts in the coastal estuarine wetland of the Yellow River Delta. Vegetation probably represents the most crucial step for the ecosystem functions of wetlands, but it is unclear how microbial populations and functions shift in pace with the colonization and succession of vegetation. In this study, we found that a monospecies genus and a multispecies genus are fastidiously predominant in the bare and vegetative wetlands of the Yellow River Delta, respectively. Consistently, photosynthesis genes were enriched exclusively in bare land, while genes involved in biological organic carbon metabolism and the cycling of main elements were highly expressed in vegetative wetlands, were mostly included in the MAG of , and were consistent with soil metabolomic results. Our results provide insight into the adaptive succession of predominant bacterial species and their ecosystem functions in response to the presence of vegetation.
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http://dx.doi.org/10.1128/mSystems.00412-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289592PMC
June 2020

Chimeric antigen receptor therapy in hematological malignancies: antigenic targets and their clinical research progress.

Ann Hematol 2020 Aug 9;99(8):1681-1699. Epub 2020 May 9.

Department of Hematology, Zhujiang Hospital, Southern Medical University, No. 253, Middle Gongye Avenue, Guangzhou, 510282, Guangdong, People's Republic of China.

Chimeric antigen receptor (CAR)-based immunotherapy has achieved dramatic success in the treatment of B cell malignancies, based on the summary of current research data, and has shown good potential in early phase cancer clinical trials. Modified constructs are being optimized to recognize and destroy tumor cells more effectively. By targeting the proper B-lineage-specific antigens such as CD19 and CD20, adoptive immunotherapy has demonstrated promising clinical results and already plays a role in the treatment of several lymphoid malignancies, which highlights the importance of target selection for other CAR therapies. The high efficacy of CAR-T cells has resulted in the approval of anti-CD19-directed CAR-T cells for the treatment of B cell malignancies. In this review, we focus on the basic structure and current clinical application of CAR-T cells, detail the research progress of CAR-T for different antigenic targets in hematological malignancies, and further discuss the current barriers and proposed solutions, investigating the possible mechanisms of recurrence of CAR-T cell therapy. A summary of the paper is also given to overview as the prospects for this therapy.
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http://dx.doi.org/10.1007/s00277-020-04020-7DOI Listing
August 2020

Highly polarization-sensitive far infrared detector based on an optical antenna integrated aligned carbon nanotube film.

Nanoscale 2020 Jun 14;12(22):11808-11817. Epub 2020 Apr 14.

School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, China.

Polarization detection is another important way to characterize a light field in addition to intensity and spectrum. This is required for high-fidelity information acquisition and high precision target recognition in the infrared detection region. Single-wall carbon nanotube (SWCNT) films have been investigated for infrared detection with considerable sensitivity at room temperature based on the photothermoelectric effect. In this work, a bowtie antenna integrated aligned SWCNT film is proposed for highly polarization sensitive, far infrared detection. The SWCNT film is shaped into a belt and doped with diverse agents to form a p-n junction at the center. The SWCNTs are arranged perpendicular to the electronic transportation direction. The antenna is aligned at the junction and along the SWCNTs. Based on the following four factors: (1) deep-subwavelength light concentration at the junction, (2) alignment between the SWCNTs and the antenna, (3) anisotropic heat transfer in the aligned SWCNT film, and (4) light field reduction within the gap of the bowtie antenna for the polarization perpendicular to the antenna axis, the ratio between the responsivities for the polarizations parallel and perpendicular to the SWCNTs could be higher than 13 600. By changing the size of the antenna, the resonant frequency could be tuned. Over the range from 0.5 to 1.5 THz, the peak polarization extinction ratios at different resonant frequencies are all bigger than 700, and they are 16 to 320 times higher than that of the aligned SWCNT belt without the antenna. Moreover, the integration of the antenna and the aligned SWCNT belt also enhances the responsivity by 1 to 2 orders of magnitude. Compared to an aligned multi-wall carbon nanotube (MWCNT) film, an aligned SWCNT film integrated with an optical antenna is more favorable for highly polarization sensitive, far infrared detection. The result is based on the numerical simulations of the light and the thermal fields.
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http://dx.doi.org/10.1039/d0nr01243bDOI Listing
June 2020

Clinical characteristics and immunosuppressant management of coronavirus disease 2019 in solid organ transplant recipients.

Am J Transplant 2020 07 4;20(7):1916-1921. Epub 2020 May 4.

Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, Hubei, China.

Over 1 000 000 cases of coronavirus disease 2019 (COVID-19) have been confirmed since the worldwide outbreak began. Not enough data on infected solid organ transplant (SOT) recipients are available, especially data about the management of immunosuppressants. We report two cases of COVID-19 in two transplant recipients, with different treatments and prognoses. The first patient received liver transplantation due to hepatitis B virus-related hepatocellular carcinoma and was confirmed to have COVID-19 9 days later. Following a treatment regimen consisting of discontinued immunosuppressant use and low-dose methylprednisolone-based therapy, the patient developed acute rejection but eventually recovered. The other patient had undergone a renal transplant from a living-related donor 17 years ago, and was admitted to the hospital because of persistent fever. This patient was also diagnosed with COVID-19. His treatment regimen consisted of reduced immunosuppressant use. No signs of rejection were observed during the regimen. In the end, the patient successfully recovered from COVID-19. These effectively treated cases can provide a basis for immunosuppressant management of COVID-19-positive SOT recipients.
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http://dx.doi.org/10.1111/ajt.15928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262295PMC
July 2020

The chemical profile of active fraction of Kalimeris indica and its quantitative analysis.

Biomed Chromatogr 2020 Jul 31;34(7):e4828. Epub 2020 Mar 31.

The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, China.

Kalimeris indica (L) Sch-Bip is a medicinal plant used by the Miao ethnic group in the Guizhou province of China. It is widely used as a fresh vegetable to treat colds, diarrhea and gastric ulcers. However, few studies have been conducted on the mechanism of its effect on colds, and its quality control. The anticomplement and antitussive activities of different polar extracts of K. indica were evaluated. Fifty-nine compounds, mainly including phenols and flavonoids, were identified in K. indica extract by ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry. A method was established through ultra-high-performance liquid chromatography with a photodiode array to simultaneously determine the anticomplement and antitussive activity of five compounds in K. indica combining chemical identification with chemometrics for discrimination and quality assessment. Also, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid exhibited significantly higher anticomplementary activity than the other three compounds. The quantitative data were further analyzed by principal component analysis and orthogonal partial least-squares discriminant analysis. Heatmap visualization was conducted to clarify the distribution of the major compounds in different geographical origins. Screening pharmacological activities by a combination of chemometrics and chemical identification might be an effective method for the quality control of K. indica.
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http://dx.doi.org/10.1002/bmc.4828DOI Listing
July 2020