Publications by authors named "Zhicheng Li"

60 Publications

Cancer care disruption and reorganisation during the COVID-19 pandemic in Australia: A patient, carer and healthcare worker perspective.

PLoS One 2021 17;16(9):e0257420. Epub 2021 Sep 17.

Daffodil Centre, The University of Sydney, a joint venture with Cancer Council New South Wales, Sydney, NSW, Australia.

The COVID-19 pandemic has dramatically impacted cancer care worldwide. Disruptions have been seen across all facets of care. While the long-term impact of COVID-19 remains unclear, the immediate impacts on patients, their carers and the healthcare workforce are increasingly evident. This study describes disruptions and reorganisation of cancer services in Australia since the onset of COVID-19, from the perspectives of people affected by cancer and healthcare workers. Two separate online cross-sectional surveys were completed by: a) cancer patients, survivors, carers, family members or friends (n = 852) and b) healthcare workers (n = 150). Descriptive analyses of quantitative survey data were conducted, followed by inductive thematic content analyses of qualitative survey responses relating to cancer care disruption and perceptions of telehealth. Overall, 42% of cancer patients and survivors reported experiencing some level of care disruption. A further 43% of healthcare workers reported atypical delays in delivering cancer care, and 50% agreed that patient access to research and clinical trials had been reduced. Almost three quarters (73%) of patients and carers reported using telehealth following the onset of COVID-19, with high overall satisfaction. However, gaps were identified in provision of psychological support and 20% of participants reported that they were unlikely to use telehealth again. The reorganisation of cancer care increased the psychological and practical burden on carers, with hospital visitation restrictions and appointment changes reducing their ability to provide essential support. COVID-19 has exacerbated a stressful and uncertain time for people affected by cancer and healthcare workers. Service reconfiguration and the adoption of telehealth have been essential adaptations for the pandemic response, offering long-term value. However, our findings highlight the need to better integrate psychosocial support and the important role of carers into evolving pandemic response measures. Learnings from this study could inform service improvements that would benefit patients and carers longer-term.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0257420PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448370PMC
September 2021

Biomimetic Mechanoswitchable Interfaces for High-Performance Spatial Gas Bubble Maneuvering.

ACS Appl Mater Interfaces 2021 Sep 3;13(36):43769-43776. Epub 2021 Sep 3.

CAS Key Laboratory of Mechanical Behavior and Design of Materials, Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education Institutes, Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230026, China.

The on-demand manipulation of gas bubbles in aqueous ambient environments is fundamental to many fields such as microfluidics and biochemical microanalysis. However, most bubble manipulation strategies are limited to restricted locomotion on the confined surfaces without spatial convenience of transport. Herein, we report a kind of biomimetic bubble manipulator with mechanoswitchable interfaces (MSIs), featuring the advantages of parallel bubble control and spatial maneuvering flexibility. By the synergic action between Janus aluminum membrane and superaerophilic microfiber array, the gas-MSI interfacial adhesion can be reversibly switched to achieve capturing/releasing underwater bubbles. Moreover, the adhesion force of MSI can be readily tuned by diverse experimental parameters including surface roughness, fiber number, diameter, and spacing of the neighboring microfibers, which are further systematically investigated. Relying on this mobile platform, we demonstrate a series of powerful applications including bubble parallel control, bubble array regrouping, arbitrary bubble transport and even manipulating underwater solids through bubbles, which are otherwise challenging for conventional approaches. We envision that this versatile platform will bring new insights into potential applications, such as cross-species sample control and handheld gas microsyringe.
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http://dx.doi.org/10.1021/acsami.1c13527DOI Listing
September 2021

The Effect of Virtual Reality Training on Anticipatory Postural Adjustments in Patients with Chronic Nonspecific Low Back Pain: A Preliminary Study.

Neural Plast 2021 27;2021:9975862. Epub 2021 Jul 27.

Department of Rehabilitation Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.

Objectives: This study is aimed at exploring the effects of virtual reality (VR) training on postural control, measured by anticipatory and compensatory postural adjustments (APAs and CPAs, respectively), in patients with chronic nonspecific low back pain (CNLBP) and the potential neuromuscular mechanism of VR training.

Methods: Thirty-four patients were recruited and randomly assigned to the VR group ( = 11), the motor control exercise group (MCE, = 12) and the control group (CG, = 11). The VR group received VR training using Kinect Xbox 360 systems and magnetic therapy. Besides magnetic therapy, the participants in the MCE group performed real-time ultrasound-guided abdominal drawing-in maneuver (ADIM) and four-point kneeling exercise. The CG only received magnetic therapy. Surface muscle electromyography (sEMG) was used to record the muscle activities of transverse abdominis (TrA), multifidus (MF), lateral gastrocnemius (LG), and tibialis anterior (TA) during ball-hitting tasks. The muscle activation time and integrals of the electromyography activities (IEMGs) during the APA and CPA stages were calculated and used in the data analysis. The visual analogue scale (VAS) and Oswestry dysfunction index (ODI) scores were also recorded.

Results: A significant interaction effect of time × group was observed on the activation time of TrA ( = 0.018) and MF ( = 0.037). The post-intervention activation time of the TrA was earlier in the VR group ( = 0.029). In contrast, the post-intervention activation time of the MF was significantly delayed in the VR group ( = 0.001). The IEMGs of TrA ( = 0.002) and TA ( = 0.007) during CPA1 significantly decreased only in the VR group after the intervention. The VAS scores of three group participants showed significant decreases after intervention ( < 0.001).

Conclusions: Patients with CNLBP showed reciprocal muscle activation patterns of the TrA and MF muscles after VR training. VR training may be a potential intervention for enhancing the APAs of the patients with CNLBP.
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http://dx.doi.org/10.1155/2021/9975862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8337111PMC
July 2021

Magnetism-Actuated Superhydrophobic Flexible Microclaw: From Spatial Microdroplet Maneuvering to Cross-Species Control.

ACS Appl Mater Interfaces 2021 Jul 13;13(29):35165-35172. Epub 2021 Jul 13.

CAS Key Laboratory of Mechanical Behavior and Design of Materials, Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education Institutes, Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230026, China.

The flexible maneuvering of microliter liquid droplets is significant in both fundamental science and practical applications. However, most current strategies are limited to the rigid locomotion on confined geographies platforms, which greatly hinder their practical uses. Here, we propose a magnetism-actuated superhydrophobic flexible microclaw (MSFM) with hierarchical structures for water droplet manipulation. By virtue of precise femtosecond laser patterning on magnetism-responsive poly(dimethylsiloxane) (PDMS) films doped with carbonyl iron powder, this MSFM without chemical contamination exhibits powerful spatial droplet maneuvering advantages with fast response (<100 ms) and lossless water transport (∼50 cycles) in air. We further performed quantitative analysis of diverse experimental parameters including petal number, length, width, and iron element proportion in MSFM impacting the applicable maneuvering volumes. By coupling the advantages of spatial maneuverability and fast response into this versatile platform, typical unique applications are demonstrated such as programmable coalescence of droplets, collecting debris via droplets, tiny solid manipulation in aqueous severe environments, and harmless living creature control. We envision that this versatile MSFM should provide great potential for applications in microfluidics and cross-species robotics.
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http://dx.doi.org/10.1021/acsami.1c09142DOI Listing
July 2021

Proliferated and Accelerated Accumulation of Suberin Poly Phenolic and Lignin at Wound Sites of Potato Tubers.

Foods 2021 Jun 4;10(6). Epub 2021 Jun 4.

College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.

is a type of biocontrol yeast offering effective postharvest disease control. To the best of our knowledge, the effect of upon the healing of tubers is yet to be studied. The present study addresses the existing knowledge gap by investigating the effect of on wound healing in potato tubers. The results show that colonized and proliferated at the wound sites during the early and intermediate stages of healing. In addition, reduced weight loss of wounded tubers, decreased disease index of inoculated tubers with , and accelerated accumulation of suberin poly phenolic (SPP) and lignin at wound sites. activated phenylpropanoid metabolism and increased the content of SPP monomers, total phenol, flavonoids, and lignin. Furthermore, the yeast increased HO content as well as peroxidase activity.
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http://dx.doi.org/10.3390/foods10061286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230253PMC
June 2021

Effects of nitric oxide treatment on lignin biosynthesis and texture properties at wound sites of muskmelons.

Food Chem 2021 Nov 25;362:130193. Epub 2021 May 25.

College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China; Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Rishon LeZion, Israel.

Lignin is an important component of the healing tissue in fruits. In this study, we treated muskmelon (Cucumis melo L. cv. "Manao") fruit with exogenous nitric oxide (NO) donor sodium nitroprusside (SNP) to observe and analyze its effect on lignin synthesis and accumulation during healing. Results showed that SNP treatment enhanced the contents of endogenous NO and HO, increased the activities of phenylalanine ammonia lyase, cinnamate 4 hydroxylase, cinnamyl alcohol dehydrogenase, and peroxidase, and raised the contents of sinapyl alcohol, coniferyl alcohol, coumaryl alcohol, and lignin. SNP augmented the hardness of the healing tissue and decreased its resilience, springiness, and cohesiveness. In addition, SNP treatment effectively reduced the weight loss and disease index of wounded muskmelons. All these results suggest that lignin metabolism mediated by NO play a crucial role in wound healing of muskmelons.
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http://dx.doi.org/10.1016/j.foodchem.2021.130193DOI Listing
November 2021

Self-assembled mesostructured CoFeO nanoparticle superstructures for highly efficient oxygen evolution.

J Colloid Interface Sci 2021 Jul 9;593:125-132. Epub 2021 Mar 9.

Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200438, China. Electronic address:

Self-assembly of colloidal nanoparticles (NPs) into well-defined superstructures has been recognized as one of the most promising ways to fabricate rationally-designed functional materials for a variety of applications. Introducing hierarchical mesoporosity into NP superstructures will facilitate mass transport while simultaneously enhancing the accessibility of constituent NPs, which is of critical importance for widening their applications in catalysis and energy-related fields. Herein, we develop a colloidal co-assembly strategy to construct mesostructured, carbon-coated CoFeO NP superstructures ([email protected]), which show great promise as highly efficient electrocatalysts for the oxygen evolution reaction (OER). Specifically, organically-stabilized SiO NPs are employed as both building blocks and sacrificial template, which co-assemble with CoFeO NPs to afford binary NP superstructures through a solvent drying process. [email protected] are obtainable after in situ ligand carbonization followed by the selective removal of SiO NPs. The hierarchical mesoporous structure of [email protected], combined with the conformal graphitic carbon coating derived from the native organic ligands, significantly improves their electrocatalytic performance as OER electrocatalysts when compared with nonporous CoFeO NP superstructures. This work establishes a new and facile approach for designing NP superstructures with hierarchical mesoporosity, which may find wide applications in energy storage and conversion.
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http://dx.doi.org/10.1016/j.jcis.2021.02.126DOI Listing
July 2021

Dihydromyricetin Exhibits Antitumor Activity in Nasopharyngeal Cancer Cell Through Antagonizing Wnt/β-catenin Signaling.

Integr Cancer Ther 2021 Jan-Dec;20:1534735421991217

The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China.

Background: Cancer stem cells (CSCs) have been demonstrated to play a vital role in a diversity of biological processes in cancers. With the emergence of new evidence, the important function of CSCs in the formation of multidrug resistance of nasopharyngeal cancer has been demonstrated. Dysregulated Wnt/β-catenin signaling pathway is an important contributor to chemoresistance and maintenance of CSCs-like characteristics. This research aims to investigate comprehensively the function of dihydromyricetin (DMY), a natural flavonoid drug, on the cisplatin (cis) resistance and stem cell properties of nasopharyngeal cancer.

Methods: In this study, the functional role of DMY in nasopharyngeal cancer progression was comprehensively investigated in vitro and in vivo, and then its relationship with CSCs-like phenotypes and multiple oncogenes was analyzed.

Results: In parallel assays, the growth inhibitory action of cis was enhanced by the addition of DMY in cis-resistant nasopharyngeal cancer cell lines (Hone1/cis and CNE1/cis). Functional assays showed that DMY markedly diminished the stem cell properties of nasopharyngeal cells, such as colony and tumor-sphere formation. In vivo data showed that the growth of Hone1 CSCs formed tumor xenograft was inhibited significantly by the administration of DMY. Additionally, DMY could impair the Wnt/β-catenin signaling pathway and regulate the expression of downstream proteins in nasopharyngeal cancer cells.

Conclusions: Our study clarified the anti-tumor activity of DMY through blocking the Wnt/β-catenin signaling pathway in nasopharyngeal cancer. Therefore, DMY could be a novel therapeutic agent for nasopharyngeal cancer treatment.
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http://dx.doi.org/10.1177/1534735421991217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7975991PMC
October 2021

Psychosocial impact of COVID-19 on cancer patients, survivors, and carers in Australia: a real-time assessment of cancer support services.

Support Care Cancer 2021 Sep 11;29(9):5463-5473. Epub 2021 Mar 11.

Cancer Research Division, Cancer Council NSW, 153 Dowling Street, Sydney, NSW, 2011, Australia.

Purpose: This study aimed to explore the psychosocial impacts of the coronavirus disease (COVID-19) pandemic on cancer patients, survivors, and carers in Australia.

Methods: Using real-time insights from two Cancer Council NSW services-131120 Information and Support Line and Online Community (CCOC) forums-we assessed service demand trends, distress levels (using the distress thermometer), and content from 131120 calls and online posts between 01 December 2019 and 31 May 2020. Emergent themes were identified through an inductive conventional content analysis with 131120 call notes, followed by a deductive directed content analysis on CCOC posts.

Results: In total, 688 COVID-19-related 131120 calls (n = 496) and online posts (n = 192) were analysed. Service demand peaked in March 2020 and self-reported distress peaked in May 2020 at an average of 8/10 [Mean = 7.5; SD = 0.9]. Five themes emerged from the qualitative analysis: psychological distress and fear of virus susceptibility, practical issues, cancer service disruptions, information needs, and carer Issues.

Conclusions: The psychosocial impacts of COVID-19 on people affected by cancer are multifaceted and likely to have long-lasting consequences. Our findings drove the development of six recommendations across three domains of support, information, and access. Cancer patients, survivors, and carers already face stressful challenges dealing with a cancer diagnosis or survivorship. The added complexity of restrictions and uncertainty associated with the pandemic may compound this. It is important that healthcare providers are equipped to provide patient-centred care during and after this crisis. Our recommendations provide points of consideration to ensure care is tailored and patient oriented.
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http://dx.doi.org/10.1007/s00520-021-06101-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946616PMC
September 2021

[Efficacy of percutaneous vagal stimulation in the treatment of chronic tinnitus].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Feb;35(2):105-108

Department of Otolaryngology,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou,510630,China.

The aim of this study is to investigate the efficacy of transcutaneous vagus nerve stimulation(taVNS) in the treatment of chronic tinnitus. taVNS was used to treat chronic decompensated tinnitus, 10 days as a course of treatment. Record and analyze the improvement of the patient's subjective symptoms during treatment. The THI, PSQI and DASS-21 scores were performed at 10 days, 3 months and 6 months after treatment. The improvement of tinnitus in taVNS group and control group at the above recorded time points was compared, and the THI score of taVNS group was compared before and after treatment. ① During the taVNS treatment, 93.65% of the patients achieved improvement in subjective symptoms, and 28.6% of the patients voluntarily accepted the second course of taVNS treatment; ② The sleep improvement was optimal when the treatment was 10 days, and some patients had poor sleep status after 3 or 6 months; ③ The anxiety of taVNS group was improved during and after treatment, and the best effect time was 3 months and 6 months after treatment; ④ There was no significant difference in THI score between the two groups, but there was significant improvement in taVNS group at 3 months and 6 months after treatment. taVNS treatment can significantly improve the sleep disorder, anxiety disorder and tinnitus disturbance of tinnitus patients, and the curative effect is good after 3 months and 6 months of prevention.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.02.003DOI Listing
February 2021

Social Support and Tinnitus Distress: The Importance of Developing Positive Psychological Qualities in Patients with Chronic Tinnitus.

Audiol Neurootol 2021 4;26(4):246-256. Epub 2021 Feb 4.

Department of Otolaryngology Head and Neck Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China,

Introduction: The role of social support in the relief of tinnitus distress and related mechanisms remains unclear. This study aimed to confirm a hypothesis that the influence of social support on tinnitus distress is mediated by resilience and self-esteem.

Methods: The Social Support Rating Scale, Connor-Davidson Resilience Scale, Positive Version of Rosenberg Self-Esteem Scale, and Tinnitus Handicap Inventory were used to assess 296 patients who experience chronic subjective tinnitus. The collected data were subjected to correlational analysis, mediating effect testing, and structural equation model analysis using R 3.3.1 with the mediate and lavaan packages.

Results: The result showed that social support had significant positive correlations with resilience and self-esteem, while resilience and self-esteem had significant negative correlations with tinnitus distress. Furthermore, social support indirectly affected tinnitus distress through the full mediating effects of resilience and self-esteem and could indirectly affect resilience through the partial mediating effects of self-esteem.

Conclusion: These results suggested that the key to social support for alleviating tinnitus distress lies in the development of patients' resilience and self-esteem. Promoting the development of positive psychological quality of tinnitus patients and improving their perception of social support may become the new approaches of clinical management of tinnitus.
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http://dx.doi.org/10.1159/000511254DOI Listing
September 2021

Evaluation of the Antidepressant Effect of the Functional Beverage Containing Active Peptides, Menthol and Eleutheroside and Investigation of Its Mechanism of Action in Mice.

Food Technol Biotechnol 2020 Sep;58(3):295-302

College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, 712100 Yangling, PR China.

Research Background: Depression has become a global threat to human health. In order to solve it, researchers have conducted multi-faceted studies including diet. Many food-derived bioactive substances have shown antidepressant effects. However, there are few studies on the design of industrialized food with antidepressant effect. This study aims to evaluate the antidepressant effect of a functional beverage made from several ingredients with potential antidepressant function and investigate its antidepressant mechanisms.

Experimental Approach: The beverage consists of peppermint oil, active peptides derived from bovine milk casein and extract (ASE) whose active ingredient is eleutheroside. Different amounts of ASE were evaluated to determine the optimal concentration of eleutheroside in this functional beverage to deliver the best antidepressant effect through extensive behavioral testing, including preliminary acute stress experiments and further chronic unpredictable mild stress test.

Results And Conclusions: The results demonstrated that the beverage with 15 mg/kg of eleutheroside could significantly reduce the mice's immobility time of tail suspension test and forced swimming test, recover mice's sucrose preference and behavior changes in the open field test, improve the contents of dopamine, norepinephrine, 5-hydroxytryptamine and the activity of superoxide dismutase and reduce the content of malondialdehyde in mice's brains, which indicated that the improvement of monoamine neurotransmitter systems and antioxidation was one potential mechanism of antidepressant action.

Novelty And Scientific Contribution: This study provides a design of antidepressant functional beverage and an efficient way for the prevention and treatment of depression.
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http://dx.doi.org/10.17113/ftb.58.03.20.6568DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709459PMC
September 2020

Radiomic Features From Multi-Parameter MRI Combined With Clinical Parameters Predict Molecular Subgroups in Patients With Medulloblastoma.

Front Oncol 2020 2;10:558162. Epub 2020 Oct 2.

Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

The 2016 WHO classification of central nervous system tumors has included four molecular subgroups under medulloblastoma (MB) as sonic hedgehog (SHH), wingless (WNT), Grade 3, and Group 4. We aimed to develop machine learning models for predicting MB molecular subgroups based on multi-parameter magnetic resonance imaging (MRI) radiomics, tumor locations, and clinical factors. A total of 122 MB patients were enrolled retrospectively. After selecting robust, non-redundant, and relevant features from 5,529 extracted radiomics features, a random forest model was constructed based on a training cohort ( = 92) and evaluated on a testing cohort ( = 30). By combining radiographic features and clinical parameters, two combined prediction models were also built. The subgroup can be classified using an 11-feature radiomics model with a high area under the curve (AUC) of 0.8264 for WNT and modest AUCs of 0.6683, 0.6004, and 0.6979 for SHH, Group 3, and Group 4 in the testing cohort, respectively. Incorporating location and hydrocephalus into the radiomics model resulted in improved AUCs of 0.8403 and 0.8317 for WNT and SHH, respectively. After adding gender and age, the AUCs for WNT and SHH were further improved to 0.9097 and 0.8654, while the accuracies were 70 and 86.67% for Group 3 and Group 4, respectively. Prediction performance was excellent for WNT and SHH, while that for Group 3 and Group 4 needs further improvements. Machine learning algorithms offer potentials to non-invasively predict the molecular subgroups of MB.
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http://dx.doi.org/10.3389/fonc.2020.558162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566191PMC
October 2020

Incremental prognostic value and underlying biological pathways of radiomics patterns in medulloblastoma.

EBioMedicine 2020 Nov 21;61:103093. Epub 2020 Oct 21.

Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Jian she Dong Road 1, Zhengzhou, Henan 450052, China. Electronic address:

Background: To develop a radiomics signature for predicting overall survival (OS)/progression-free survival (PFS) in patients with medulloblastoma (MB), and to investigate the incremental prognostic value and biological pathways of the radiomics patterns.

Methods: A radiomics signature was constructed based on magnetic resonance imaging (MRI) from a training cohort (n = 83), and evaluated on a testing cohort (n = 83). Key pathways associated with the signature were identified by RNA-seq (GSE151519). Prognostic value of pathway genes was assessed in a public GSE85218 cohort.

Findings: The radiomics-clinicomolecular signature predicted OS (C-index 0.762) and PFS (C-index 0.697) better than either the radiomics signature (C-index: OS: 0.649; PFS: 0.593) or the clinicomolecular signature (C-index: OS: 0.725; PFS: 0.691) alone, with a better calibration and classification accuracy (net reclassification improvement: OS: 0.298, P = 0.022; PFS: 0.252, P = 0.026). Nine pathways were significantly correlated with the radiomics signature. Average expression value of pathway genes achieved significant risk stratification in GSE85218 cohort (log-rank P = 0.016).

Interpretation: This study demonstrated radiomics signature, which associated with dysregulated pathways, was an independent parameter conferring incremental value over clinicomolecular factors in survival predictions for MB patients.

Funding: A full list of funding bodies that contributed to this study can be found in the Acknowledgements section.
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http://dx.doi.org/10.1016/j.ebiom.2020.103093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581926PMC
November 2020

A computational toolset for rapid identification of SARS-CoV-2, other viruses and microorganisms from sequencing data.

Brief Bioinform 2021 03;22(2):924-935

department of research and development, HaploX Biotechnology. His research interests lie mainly in next-generation sequencing and bioinformatics.

In this paper, we present a toolset and related resources for rapid identification of viruses and microorganisms from short-read or long-read sequencing data. We present fastv as an ultra-fast tool to detect microbial sequences present in sequencing data, identify target microorganisms and visualize coverage of microbial genomes. This tool is based on the k-mer mapping and extension method. K-mer sets are generated by UniqueKMER, another tool provided in this toolset. UniqueKMER can generate complete sets of unique k-mers for each genome within a large set of viral or microbial genomes. For convenience, unique k-mers for microorganisms and common viruses that afflict humans have been generated and are provided with the tools. As a lightweight tool, fastv accepts FASTQ data as input and directly outputs the results in both HTML and JSON formats. Prior to the k-mer analysis, fastv automatically performs adapter trimming, quality pruning, base correction and other preprocessing to ensure the accuracy of k-mer analysis. Specifically, fastv provides built-in support for rapid severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) identification and typing. Experimental results showed that fastv achieved 100% sensitivity and 100% specificity for detecting SARS-CoV-2 from sequencing data; and can distinguish SARS-CoV-2 from SARS, Middle East respiratory syndrome and other coronaviruses. This toolset is available at: https://github.com/OpenGene/fastv.
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http://dx.doi.org/10.1093/bib/bbaa231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7543257PMC
March 2021

Confirmation of brand identification in infant formulas by using near-infrared spectroscopy fingerprints.

Anal Methods 2020 05;12(19):2469-2475

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, PR China.

In this study, a near infrared (NIR) spectroscopy fingerprinting method coupled with principal component analysis (PCA) was developed for the confirmation of brand identification in infant formulas. The NIR spectroscopy fingerprints of the Brand A infant formula were acquired in 12 000-4000 cm-1 at a sample temperature of 20 °C without pressing the sample. The contents of major nutrients of Stage 1, 2, and 3 infant formulas were compared within Brand A. The NIR spectroscopy fingerprints of Brand A Stage 1 samples were compared with those of four other brand-named Stage 1 samples, whereas the fingerprints of Brand A Stage 2 and 3 were compared with those of two of the four brands, to distinguish the differences between brands. The NIR spectroscopy fingerprinting results showed that the Brand A formula can be completely differentiated from the other brands at each stage. The combination of NIR spectroscopy fingerprinting and PCA is an effective method for the purpose of confirmation of brand identification and brand protection in infant formulas.
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http://dx.doi.org/10.1039/d0ay00375aDOI Listing
May 2020

Evaluation of reconstruction algorithms for a stationary digital breast tomosynthesis system using a carbon nanotube X-ray source array.

J Xray Sci Technol 2020 ;28(6):1157-1169

Lauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Breast cancer is the most frequently diagnosed cancer in women worldwide. Digital breast tomosynthesis (DBT), which is based on limited-angle tomography, was developed to solve tissue overlapping problems associated with traditional breast mammography. However, due to the problems associated with tube movement during the process of data acquisition, stationary DBT (s-DBT) was developed to allow the X-ray source array to stay stationary during the DBT scanning process. In this work, we evaluate four widely used and investigated DBT image reconstruction algorithms, including the commercial Feldkamp-Davis-Kress algorithm (FBP), the simultaneous iterative reconstruction technique (SIRT), the simultaneous algebraic reconstruction technique (SART) and the total variation regularized SART (SART-TV) for an s-DBT imaging system that we set up in our own laboratory for studies using a semi-elliptical digital phantom and a rubber breast phantom to determine the most superior algorithm for s-DBT image reconstruction among the four algorithms. Several quantitative indexes for image quality assessment, including the peak signal-noise ratio (PSNR), the root mean square error (RMSE) and the structural similarity (SSIM), are used to determine the best algorithm for the imaging system that we set up. Image resolutions are measured via the calculation of the contrast-to-noise ratio (CNR) and artefact spread function (ASF). The experimental results show that the SART-TV algorithm gives reconstructed images with the highest PSNR and SSIM values and the lowest RMSE values in terms of image accuracy and similarity, along with the highest CNR values calculated for the selected features and the best ASF curves in terms of image resolution in the horizontal and vertical directions. Thus, the SART-TV algorithm is proven to be the best algorithm for use in s-DBT image reconstruction for the specific imaging task in our study.
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http://dx.doi.org/10.3233/XST-200668DOI Listing
October 2021

Enhancing the X-Ray Differential Phase Contrast Image Quality With Deep Learning Technique.

IEEE Trans Biomed Eng 2021 06 21;68(6):1751-1758. Epub 2021 May 21.

Objective: The purpose of this work is to investigate the feasibility of using deep convolutional neural network (CNN) to improve the image quality of a grating-based X-ray differential phase contrast imaging (XPCI) system.

Methods: In this work, a novel deep CNN based phase signal extraction and image noise suppression algorithm (named as XP-NET) is developed. The numerical phase phantom, the ex vivo biological specimen and the ACR breast phantom are evaluated via the numerical simulations and experimental studies, separately. Moreover, images are also evaluated under different low radiation levels to verify its dose reduction capability.

Results: Compared with the conventional analytical method, the novel XP-NET algorithm is able to reduce the bias of large DPC signals and hence increasing the DPC signal accuracy by more than 15%. Additionally, the XP-NET is able to reduce DPC image noise by about 50% for low dose DPC imaging tasks.

Conclusion: This proposed novel end-to-end supervised XP-NET has a great potential to improve the DPC signal accuracy, reduce image noise, and preserve object details.

Significance: We demonstrate that the deep CNN technique provides a promising approach to improve the grating-based XPCI performance and its dose efficiency in future biomedical applications.
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http://dx.doi.org/10.1109/TBME.2020.3011119DOI Listing
June 2021

Gencore: an efficient tool to generate consensus reads for error suppressing and duplicate removing of NGS data.

BMC Bioinformatics 2019 Dec 27;20(Suppl 23):606. Epub 2019 Dec 27.

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Background: Removing duplicates might be considered as a well-resolved problem in next-generation sequencing (NGS) data processing domain. However, as NGS technology gains more recognition in clinical application, researchers start to pay more attention to its sequencing errors, and prefer to remove these errors while performing deduplication operations. Recently, a new technology called unique molecular identifier (UMI) has been developed to better identify sequencing reads derived from different DNA fragments. Most existing duplicate removing tools cannot handle the UMI-integrated data. Some modern tools can work with UMIs, but are usually slow and use too much memory. Furthermore, existing tools rarely report rich statistical results, which are very important for quality control and downstream analysis. These unmet requirements drove us to develop an ultra-fast, simple, little-weighted but powerful tool for duplicate removing and sequence error suppressing, with features of handling UMIs and reporting informative results.

Results: This paper presents an efficient tool gencore for duplicate removing and sequence error suppressing of NGS data. This tool clusters the mapped sequencing reads and merges reads in each cluster to generate one single consensus read. While the consensus read is generated, the random errors introduced by library construction and sequencing can be removed. This error-suppressing feature makes gencore very suitable for the application of detecting ultra-low frequency mutations from deep sequencing data. When unique molecular identifier (UMI) technology is applied, gencore can use them to identify the reads derived from same original DNA fragment. Gencore reports statistical results in both HTML and JSON formats. The HTML format report contains many interactive figures plotting statistical coverage and duplication information. The JSON format report contains all the statistical results, and is interpretable for downstream programs.

Conclusions: Comparing to the conventional tools like Picard and SAMtools, gencore greatly reduces the output data's mapping mismatches, which are mostly caused by errors. Comparing to some new tools like UMI-Reducer and UMI-tools, gencore runs much faster, uses less memory, generates better consensus reads and provides simpler interfaces. To our best knowledge, gencore is the only duplicate removing tool that generates both informative HTML and JSON reports. This tool is available at: https://github.com/OpenGene/gencore.
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http://dx.doi.org/10.1186/s12859-019-3280-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933617PMC
December 2019

Combining DWI radiomics features with transurethral resection promotes the differentiation between muscle-invasive bladder cancer and non-muscle-invasive bladder cancer.

Eur Radiol 2020 Mar 26;30(3):1804-1812. Epub 2019 Nov 26.

Department of Radiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, No.1630, Dongfang road, Pudong, Shanghai, 200127, China.

Purpose: To investigate the value of radiomics features from diffusion-weighted imaging (DWI) in differentiating muscle-invasive bladder cancer (MIBC) from non-muscle-invasive bladder cancer (NMIBC).

Methods: This retrospective study included 218 pathologically confirmed bladder cancer patients (training set: 131 patients, 86 MIBC; validation set: 87 patients, 55 MIBC) who underwent DWI before biopsy through transurethral resection (TUR) between July 2014 and December 2018. Radiomics models based on DWI for discriminating state of muscle-invasive were built using random forest (RF) and all-relevant (AR) methods on the training set and were tested on validation set. Combination models based on TUR data were also built. Discrimination performances were evaluated with the area under the receiver operating characteristic (ROC) curve (AUC), accuracy, sensitivity, specificity, and F1 and F2 scores. Qualitative MRI evaluation based on morphology was performed for comparison.

Results: No significant difference was found between RF and AR models. RF model was more sensitive than TUR (0.873 vs 0.655, p = 0.019) for discriminating muscle-invasive bladder cancer. When combining RF with TUR, the sensitivity increased to 0.964, significantly higher than TUR (0.655, p < 0.001), MRI evaluation (0.764, p = 0.006), and the combination of TUR and MRI (0.836, p = 0.046). Combining RF and TUR achieved the highest accuracy of 0.897 and F2 score of 0.946.

Conclusion: Combining DWI radiomics features with TUR could improve the sensitivity and accuracy in discriminating the presence of muscle invasion in bladder cancer for clinical practice. Multicenter, prospective studies are needed to confirm our results.

Key Points: • Twenty-seven to 51% of superficial bladder cancers diagnosed by transurethral resection are upstaged to muscle-invasive at radical cystectomy, suggesting its poor sensitivity for discriminating muscle-invasive bladder cancer. • A small subset of selected all-relevant radiomics features exhibited an equivalent performance compared to that of all the extracted features, confirming that radiomics data contained redundant or irrelevant features and that feature selection should be performed in building radiomics models. • Combining DWI radiomics features with transurethral resection could improve in clinical practice the sensitivity and accuracy for the detection of muscle invasion in bladder cancer.
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http://dx.doi.org/10.1007/s00330-019-06484-2DOI Listing
March 2020

Deepening our Understanding of Quality in Australia (DUQuA): An overview of a nation-wide, multi-level analysis of relationships between quality management systems and patient factors in 32 hospitals.

Int J Qual Health Care 2020 Feb;32(Supplement_1):8-21

Australian Institute of Health Innovation, Macquarie University, Level 6, 75 Talavera Road, Sydney, NSW 2109, Australia.

Objective: The Deepening our Understanding of Quality in Australia (DUQuA) project is a multisite, multi-level, cross-sectional study of 32 of the largest hospitals in Australia. This overview examines relationships between (i) organization-level quality management systems and department-level quality management strategies and (ii) patient-level measures (clinical treatment processes, patient-reported perceptions of care and clinical outcomes) within Australian hospitals.

Design: We examined hospital quality improvement structures, processes and outcomes, collecting data at organization, department and patient levels for acute myocardial infarction (AMI), hip fracture and stroke. Data sources included surveys of quality managers, clinicians and patients, hospital visits, medical record reviews and national databases. Outcomes data and patient admissions data were analysed. Relationships between measures were evaluated using multi-level models. We based the methods on the Deepening our Understanding of Quality Improvement in Europe (DUQuE) framework, extending that work in parts and customizing the design to Australian circumstances.

Setting, Participants And Outcome Measures: The 32 hospitals, containing 119 participating departments, provided wide representation across metropolitan, inner and outer regional Australia. We obtained 31 quality management, 1334 clinician and 857 patient questionnaires, and conducted 2401 medical record reviews and 151 external assessments. External data via a secondary source comprised 14 460 index patient admissions across 14 031 individual patients. Associations between hospital, Emergency Department (ED) and department-level systems and strategies and five patient-level outcomes were assessed: 19 of 165 associations (11.5%) were statistically significant, 12 of 79 positive associations (15.2%) and 7 of 85 negative associations (8.2%).

Results: We did not find clear relationships between hospital-level quality management systems, ED or department quality strategies and patient-level outcomes. ED-level clinical reviews were related to adherence to clinical practice guidelines for AMI, hip fracture and stroke, but in different directions. The results, when considered alongside the DUQuE results, are suggestive that front line interventions may be more influential than department-level interventions when shaping quality of care and that multi-pronged strategies are needed. Benchmark reports were sent to each participating hospital, stimulating targeted quality improvement activities.

Conclusions: We found no compelling relationships between the way care is organized and the quality of care across three targeted patient-level outcome conditions. The study was cross-sectional, and thus we recommend that the relationships studied should be assessed for changes across time. Tracking care longitudinally so that quality improvement activities are monitored and fed back to participants is an important initiative that should be given priority as health systems strive to develop their capacity for quality improvement over time.
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http://dx.doi.org/10.1093/intqhc/mzz103DOI Listing
February 2020

Genetic Biomarkers For Hepatocellular Carcinoma In The Era Of Precision Medicine.

J Hepatocell Carcinoma 2019 18;6:151-166. Epub 2019 Oct 18.

Department of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, People's Republic of China.

Being one of the most lethal cancers that exhibit high levels of heterogeneity, hepatocellular carcinoma (HCC) is associated with diverse oncogenic pathways underpinned by varied driver genes. HCC can be induced by different etiological factors including virus infection, toxin exposure or metabolic disorders. Consequently, patients may display varied genetic profiles, and may respond differently to the treatments involving inhibition of target pathways. These DNA/RNA mutations, copy number variations, chromatin structural changes, aberrant expression of non-coding RNAs and epigenetic modifications were considered as biomarkers in the application of precision medication. To explore how genetic testing could contribute to early diagnosis, prognosis, treatment and postoperative monitoring of HCC, we conducted a systematic review of genetic markers associated with different pathologies. Moreover, we summarized on-going clinical trials for HCC treatment, including the trials for multiple kinase inhibitors and immune checkpoint blockade (ICB). The efficacy of ICB treatment in HCC is not as good as what was observed in lung cancer and melanoma, which might be due to the heterogeneity of the microenvironment of the liver.
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http://dx.doi.org/10.2147/JHC.S224849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805787PMC
October 2019

Recovering the Missing Regions in Crystal Structures from the Nuclear Magnetic Resonance Measurement Data Using Matrix Completion Method.

J Comput Biol 2020 05 9;27(5):709-717. Epub 2019 Sep 9.

Center for Quantum Technology Research, School of Physics, Beijing Institute of Technology, Beijing, P.R. China.

Based on matrix completion algorithm, we proposed a simple method to recover the missing regions in the X-ray crystal structures using the corresponding nuclear magnetic resonance (NMR) measurement data for the proteins with both X-ray and NMR experimental data deposited in Protein Data Bank (PDB). By selecting 10 test proteins deposited in PDB and comparing with the standard MODELLER results from the root-mean-square deviation and MolProbity aspects, we validated that our method can provide a better protein structure model, which combines both X-ray crystallographic structure data and NMR data together than MODELLER algorithm. This method is particularly useful for building the initial structures in Molecular Dynamics when studying the protein folding process.
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http://dx.doi.org/10.1089/cmb.2019.0107DOI Listing
May 2020

Automatic image-domain Moiré artifact reduction method in grating-based x-ray interferometry imaging.

Phys Med Biol 2019 10 4;64(19):195013. Epub 2019 Oct 4.

Research Center for Medical Artificial Intelligence, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, People's Republic of China. Equal contributions to this work and all are considered as the first authors.

In this study, we propose to remove Moiré image artifact induced by system instabilities in grating-based x-ray interferometry imaging using convolutional neural network (CNN) technique. This method reduces Moiré image artifact in image-domain via a learned image post-processing procedure, rather than developing signal retrieval optimization algorithms to minimize the inconsistencies between acquired phase stepping data and assumed signal model. To achieve this aim, we suggested to train the CNN network using dataset synthesized from both natural images and experimentally acquired Moiré artifact-only images. In particular, a novel approach is developed to generate a large number of various high quality Moiré artifact-only images from finite groups of experimental phase stepping data. Both numerical and experimental results demonstrate that the developed CNN method is able to effectively remove the undesired Moiré image artifact. As a result, the image quality of a practical grating-based x-ray interferometry system can be greatly improved.
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http://dx.doi.org/10.1088/1361-6560/ab3c34DOI Listing
October 2019

Loss of Vision Dominance at the Preresponse Level in Tinnitus Patients: Preliminary Behavioral Evidence.

Front Neurosci 2019 14;13:482. Epub 2019 May 14.

Department of Psychology and Center for Studies of Psychological Application, South China Normal University, Guangzhou, China.

At present, the mechanisms underlying changes in visual processing in individuals with tinnitus remain unclear. Therefore, we investigated whether the vision dominance of individuals with tinnitus disappears at the preresponse level through behavioral study. A total of 38 individuals with tinnitus and 31 healthy controls completed a task in which they were asked to attend to either visual or auditory stimuli while ignoring simultaneous stimulus inputs from the other modality. We manipulated three levels of congruency between the simultaneous visual and auditory inputs: congruent (C), incongruent at the preresponse level (PRIC), and incongruent at the response level (RIC). Thus, we differentiated the cross-modal conflict explicitly into the preresponse (PRIC > C) and response (RIC > PRIC) levels. The results revealed no significant difference in the size of the preresponse level conflict between the auditory attention and visual attention conditions in tinnitus group. In brief, the preresponse level of individuals with tinnitus showed a loss in vision dominance. This may be due to the reduced interference of visual information in auditory processing.
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http://dx.doi.org/10.3389/fnins.2019.00482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527875PMC
May 2019

Scaled Alternating Steepest Descent Algorithm Applied for Protein Structure Determination from Nuclear Magnetic Resonance Data.

J Comput Biol 2019 09 22;26(9):1020-1029. Epub 2019 Apr 22.

Center for Quantum Technology Research, School of Physics, Beijing Institute of Technology, Beijing, China.

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http://dx.doi.org/10.1089/cmb.2019.0013DOI Listing
September 2019

Reducing the flammability of hydrophobic silica aerogels by doping with hydroxides.

J Hazard Mater 2019 07 28;373:536-546. Epub 2019 Mar 28.

School of Resource and Safety Engineering, Central South University, Changsha, 410083, PR China. Electronic address:

In this work, we utilized Al(OH) (AH) and Mg(OH) (MH) as dopants to reduce the flammability of hydrophobic silica aerogels (SA) and the related thermal properties and flame retardance were investigated detailedly. The TG-DSC analyses showed the thermostability of SA in MH/SA reached 512.4 °C and that for AH/SA was just 426.1 °C, both of which were higher than that of pure SA, 399.5 °C. It was known from cone calorimeter tests that the heat release rate, peak heat release rate and total heat release of AH/SA and MH/SA decreased significantly compared to that of pure SA. The time to ignition (TTI) of MH/SA was dramatically extended, reaching 20˜38 s, which was far longer than those of pure SA (˜6 s) and AH/SA (3˜8 s). The reduction in CO concentration, CO production rate and cumulative CO production verified the decreased smoke toxicity of AH/SA and MH/SA. It was further indicated that the flame-retardant effect of AH and MH correlated with their inhibitory effect on the pyrolysis of SA, while MH showed much better flame-retardant performance than that of AH. The research outcomes provide an inspiration to reduce the flammability of SA and benefit their expansion in thermal insulation field.
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http://dx.doi.org/10.1016/j.jhazmat.2019.03.112DOI Listing
July 2019

Eyes and Ears: Cross-Modal Interference of Tinnitus on Visual Processing.

Front Psychol 2018 24;9:1779. Epub 2018 Sep 24.

Center for Studies of Psychological Application and Department of Psychology, South China Normal University, Guangzhou, China.

The visual processing capacity of tinnitus patients is worse than normal controls, indicating cross-modal interference. However, the mechanism underlying the tinnitus-modulated visual processing is largely unclear. In order to explore the influence of tinnitus on visual processing, this study used a signal recognition paradigm to observe whether the tinnitus group would display a significantly longer reaction time in processing the letter symbols (Experiment 1) and emotional faces (Experiment 2) than the control group. Signal detection and signal recognition, which reflect the perceptual and conceptual aspects of visual processing respectively, were manipulated individually in different conditions to identify the pattern of the cross-modal interference of tinnitus. The results showed that the tinnitus group required a significantly prolonged reaction time in detecting and recognizing the letter symbols and emotional faces than the control group; meanwhile, no between-group difference was detected in signal encoding. In addition, any gender- and distress-modulated effects of processing were not found, suggesting the universality of the present findings. Finally, follow-up studies would be needed to explore the neural mechanism behind the decline in speed of visual processing. The positive emotional bias in tinnitus patients also needs to be further verified and discussed. : - The bottom-up visual processing speed is decreased in tinnitus patients. - Tinnitus primarily interferes with the detection of the visual signals in individuals.
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http://dx.doi.org/10.3389/fpsyg.2018.01779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6166004PMC
September 2018

Comparison of Transferred Deep Neural Networks in Ultrasonic Breast Masses Discrimination.

Biomed Res Int 2018 21;2018:4605191. Epub 2018 Jun 21.

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

This research aims to address the problem of discriminating benign cysts from malignant masses in breast ultrasound (BUS) images based on Convolutional Neural Networks (CNNs). The biopsy-proven benchmarking dataset was built from 1422 patient cases containing a total of 2058 breast ultrasound masses, comprising 1370 benign and 688 malignant lesions. Three transferred models, InceptionV3, ResNet50, and Xception, a CNN model with three convolutional layers (CNN3), and traditional machine learning-based model with hand-crafted features were developed for differentiating benign and malignant tumors from BUS data. Cross-validation results have demonstrated that the transfer learning method outperformed the traditional machine learning model and the CNN3 model, where the transferred InceptionV3 achieved the best performance with an accuracy of 85.13% and an AUC of 0.91. Moreover, classification models based on deep features extracted from the transferred models were also built, where the model with combined features extracted from all three transferred models achieved the best performance with an accuracy of 89.44% and an AUC of 0.93 on an independent test set.
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http://dx.doi.org/10.1155/2018/4605191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033250PMC
January 2019

Collaboration in a competitive healthcare system: negotiation 101 for clinicians.

J Health Organ Manag 2018 Apr 20;32(2):263-278. Epub 2018 Mar 20.

Clayton Utz, Brisbane, Australia.

Purpose The purpose of this paper is to evaluate the effectiveness of negotiation training delivered to senior clinicians, managers and executives, by exploring whether staff members implemented negotiation skills in their workplace following the training, and if so, how and when. Design/methodology/approach This is a qualitative study involving face-to-face interviews with 18 senior clinicians, managers and executives who completed a two-day intensive negotiation skills training course. Interviews were transcribed verbatim, and inductive interpretive analysis techniques were used to identify common themes. Research setting was a large tertiary care hospital and health service in regional Australia. Findings Participants generally reported positive affective and utility reactions to the training, and attempted to implement at least some of the skills in the workplace. The main enabler was provision of a Negotiation Toolkit to assist in preparing and conducting negotiations. The main barrier was lack of time to reflect on the principles and prepare for upcoming negotiations. Participants reported that ongoing skill development and retention were not adequately addressed; suggestions for improving sustainability included provision of refresher training and mentoring. Research limitations/implications Limitations include self-reported data, and interview questions positively elicited examples of training translation. Practical implications The training was well matched to participant needs, with negotiation a common and daily activity for most healthcare professionals. Implementation of the skills showed potential for improving collaboration and problem solving in the workplace. Practical examples of how the skills were used in the workplace are provided. Originality/value To the authors' knowledge, this is the first international study aimed at evaluating the effectiveness of an integrative bargaining negotiation training program targeting executives, senior clinicians and management staff in a large healthcare organization.
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http://dx.doi.org/10.1108/JHOM-12-2017-0333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925852PMC
April 2018
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