Publications by authors named "Zhi-Qiang Yu"

44 Publications

MRCKβ links Dasm1 to actin rearrangements to promote dendrite development.

J Biol Chem 2021 Apr 29:100730. Epub 2021 Apr 29.

NHC Key Laboratory of Glycoconjugate Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China;. Electronic address:

Proper dendrite morphogenesis and synapse formation are essential for neuronal development and function. Dasm1, a member of the immunoglobulin superfamily, is known to promote dendrite outgrowth and excitatory synapse maturation in vitro. However, the in vivo function of Dasm1 in neuronal development and the underlying mechanisms are not well understood. To learn more, Dasm1 knockout mice were constructed and employed to confirm that Dasm1 regulates dendrite arborization and spine formation in vivo. We performed a yeast two-hybrid screen using Dasm1, revealing MRCKβ as a putative partner; additional lines of evidence confirmed this interaction and identified cytoplasmic proline-rich region (823-947 aa) of Dasm1 and MRCKβ self-activated kinase domain (CC1, 410-744 aa) as necessary and sufficient for binding. Using co-immunoprecipitation assay, auto-phosphorylation assay and BS3 cross-linking assay, we show that Dasm1 binding triggers a change in MRCKβ's conformation and subsequent dimerization, resulting in auto-phosphorylation and activation. Activated MRCKβ in turn phosphorylates a class 2 regulatory myosin light chain (MLC2), which leads to enhanced actin rearrangement, causing the dendrite outgrowth and spine formation observed before. Removal of Dasm1 in mice leads to behavioral abnormalities. Together, these results reveal a crucial molecular pathway mediating cell surface and intracellular signaling communication to regulate actin dynamics and neuronal development in the mammalian brain.
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http://dx.doi.org/10.1016/j.jbc.2021.100730DOI Listing
April 2021

Modification-bioremediation of copper, lead, and cadmium-contaminated soil by combined ryegrass (Lolium multiflorum Lam.) and Pseudomonas aeruginosa treatment.

Environ Sci Pollut Res Int 2020 Oct 30;27(30):37668-37676. Epub 2020 Jun 30.

College of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China.

The principal objective of this study was to investigate the strengthened remediation effect and relevant mechanism of P. aeruginosa on ryegrass (Lolium multiflorum Lam.) for soil contaminated by Cu-Pb-Cd compound heavy metals. The results showed that the complex heavy metals' contamination had remarkable inhibiting effect on the growth of plants (P < 0.01), and the biomass of ryegrass's stem and leaves declined by 28.2%, while that of roots decreased by 34.7% after 45 days. The inoculation of P. aeruginosa promoted the growth of ryegrass in polluted soil, in which the biomass recovered to the same level of that in normal plant; the activity of both catalase and urease in the soil also increased strikingly (by 29.3% and 75.7%, respectively); the ratio of residual heavy metals in the soil decreased, while the acid extractable heavy metals increased notably. Therefore, the absorption and accumulation of ryegrass to the heavy metals in soil were improved to some extent; the bioconcentration factor of Cu, Pb, and Cd in ryegrass increased by 35.9%, 55.6%, and 283.5%, respectively. The exterior microorganism allowed the accumulation of Cu, Pb, and Cd in shoots of ryegrass increasing remarkably, while in roots, only the accumulation of Pb increased by 16.3%, and that of both Cu and Cd decreased. Besides, in the P. aeruginosa-inoculated system, the transfer factor of Cu and Cd in plants increased strikingly, while that of Pb decreased.
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http://dx.doi.org/10.1007/s11356-020-09846-2DOI Listing
October 2020

Synergistic Chemotherapy for Breast Cancer and Breast Cancer Brain Metastases via Paclitaxel-Loaded Oleanolic Acid Nanoparticles.

Mol Pharm 2020 04 17;17(4):1343-1351. Epub 2020 Mar 17.

School of Pharmaceutical Sciences, Southern Medical University, 1838 Shatai Road, Guangzhou 510515, P. R. China.

Breast cancer is the most common type of cancer in women. About 12% of all women in the United States will be diagnosed with breast cancer over their lifetimes. At the same time, incidences of brain metastases (BMs) are increasing and represent an emerging health threat. However, there is no effective chemotherapy for breast cancer brain metastases (BCBMs), which is largely due to lack of efficient delivery of antitumor drugs or drug combinations to the brain. In this study, oleanolic acid (OA), a natural pentacyclic triterpenoid compound with excellent antitumor activity, was found to form nanoparticles (NPs) and efficiently penetrate the brain for BCBMs treatment. On the basis of these findings, we developed a synergistic combinatorial chemotherapeutic regimen by formulating paclitaxel (PTX) into OA NPs and demonstrated that the resulting PTX-OA NPs effectively inhibited primary breast cancer and BCBMs in mouse xenografts. Collectively, this study introduces a new direction to treat primary breast cancer and BCBMs through noninvasive combination chemotherapy.
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http://dx.doi.org/10.1021/acs.molpharmaceut.0c00044DOI Listing
April 2020

Synergistic Platinum(II) Prodrug Nanoparticles for Enhanced Breast Cancer Therapy.

Mol Pharm 2020 04 24;17(4):1300-1309. Epub 2020 Feb 24.

State Key Laboratory of Natural Medicines, Department of Pharmaceutics, China Pharmaceutical University, Nanjing 210009, China.

Chemotherapy still accounts for a large proportion of the treatments of tumors, but the drug resistance and side effects caused by long-term chemotherapy should not be underestimated. In this work, the drug combination strategy has been widely developed to overcome the side effects brought by the use of single drugs and improve the therapeutic effect. However, in clinical applications, the co-delivery of drugs is very difficult, and different kinetics due to different drug properties will lead to a decrease in efficacy. Thus, the design of novel antitumor therapeutic agents, including new platinum agents, represents an area in need of urgent attention. Our investigation implies a promising strategy for the design of a platinum prodrug to enhance the treatment of breast cancer. A dual-drug delivery nanoparticle was developed for enhanced treatment of breast cancer based on a two-into-one co-delivery strategy. Through the synergistic effect of released cisplatin hydrate and tolfenamic acid (COX-2 inhibitor) from the coordination prodrug, the tumor growth is significantly suppressed, and the survival time is greatly extended in breast tumor-bearing mice.
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http://dx.doi.org/10.1021/acs.molpharmaceut.9b01318DOI Listing
April 2020

A Cell Membrane-Targeting Self-Delivery Chimeric Peptide for Enhanced Photodynamic Therapy and In Situ Therapeutic Feedback.

Adv Healthc Mater 2020 01 25;9(1):e1901100. Epub 2019 Nov 25.

Department of Pharmaceutical Sciences and Guangdong Key Laboratory of New Drug Screening Southern Medical University, Guangzhou, 510515, P. R. China.

Nowadays, cell membrane-targeted therapy, which owns high antitumor efficacy by avoiding cell barriers, has received great attention. Here, a cell membrane-targeted self-delivery theranostic chimeric peptide CMP-PpIX is designed for simultaneously targeted photodynamic therapy (PDT) of tumor and real-time therapeutic feedback. Self-assembled CMP-PpIX nanoparticles can effectively accumulate in tumor by enhanced permeability and retention effect without additional vector. And this chimeric peptide CMP-PpIX has low background fluorescence, which is due to its relatively high intramolecular Förster resonance energy transfer (FRET) quenching efficiency between 5(6)-carboxyfluorescein (FAM) and 4-(dimethylaminoazo)-benzene-4-carboxylic acid (Dabcyl). More importantly, CMP-PpIX can be anchored on the tumor cell membrane for more than 8 h. Under irradiation, reactive oxygen species produced by CMP-PpIX directly damage cell membrane and rapidly induce apoptosis, which significantly improve the efficacy of PDT in vitro and in vivo. Then, peptide sequence Asp-Glu-Val-Asp (DEVD) is subsequently cleaved by activated caspase-3 and activated caspase-7, which separates the FAM and Dabcyl and terminates the FRET process. Therefore, fluorescence of FAM is recovered to monitor the expression of activated caspase-3 in vitro and in vivo to feedback real-time PDT therapeutic efficacy. In general, a novel cell membrane-targeted self-delivery theranostic chimeric peptide offers new promise for effective imaging-guided PDT.
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http://dx.doi.org/10.1002/adhm.201901100DOI Listing
January 2020

Synthesis of Bioreducible Polycations with Controlled Topologies.

Methods Mol Biol 2019 ;1943:27-38

University of Nebraska Medical Center, Omaha, NE, USA.

Bioreducible polycations, which possess disulfide linkages in the backbone, have emerged as promising nucleic acid delivery carriers due to their high stability in extracellular physiological condition and bioreduction-triggered release of the genetic material. Further benefits of bioreducible polycations include decreased cytotoxicity due to intracellular reducing environment in the cytoplasm that contains high levels of reducing molecules such as glutathione. Here, we describe the synthesis of bioreducible polycations with emphasis on methods to control their topology.
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http://dx.doi.org/10.1007/978-1-4939-9092-4_2DOI Listing
July 2019

Safety and satisfaction of myopic small-incision lenticule extraction combined with monovision.

BMC Ophthalmol 2018 May 31;18(1):131. Epub 2018 May 31.

Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.

Background: To investigate the safety and optical quality of small-incision lenticule extraction (SMILE) combined with monovision, and patient satisfaction with the procedure.

Methods: The present study assessed a non-random case series involving 60 eyes of 30 patients (mean age 45.53 ± 3.20 years [range 41 to 52 years]) treated bilaterally using the VisuMax 500 system (Carl Zeiss Meditec, Jena, Germany) between January and July 2016. The target refraction was plano for the distance eye, and between - 0.5 and - 1.75 diopters (D) for the near eye. Visual acuity, refraction errors, ocular aberrations, and satisfaction questionnaire scores were calculated 1 year after surgery.

Results: All surgeries were uneventful, with a mean safety index of 1.03 and 1.04 in dominant and nondominant eyes, respectively. Binocular uncorrected distance visual acuity of all patients was ≥20/32, while binocular uncorrected near visual acuity was ≥20/40 1 year postoperatively. Higher-order aberration (0.45 ± 0.14, 0.51 ± 0.15 μm), spherical (0.18 ± 0.15, 0.21 ± 0.14 μm) and coma aberration (0.31 ± 0.16, 0.27 ± 0.17 μm) were identical between dominant and nondominant eyes after surgery. The overall satisfaction rate was 86.7% (26/30), with large contributions from age (OR = 1.76 95% CI: 1.03-2.53; P = 0.036). Binocular uncorrected distance visual acuity was related to preoperative spherical diopter (r = - 0.500; P = 0.005).

Conclusions: Monovision appears to be a safe and effective option for myopia patients with presbyopia who are considering the SMILE procedure. Patients with younger age were more satisfied with the procedure.
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http://dx.doi.org/10.1186/s12886-018-0794-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5984466PMC
May 2018

Cap morphology after small-incision lenticule extraction and its effects on intraocular scattering.

Int J Ophthalmol 2018 18;11(3):456-461. Epub 2018 Mar 18.

Eye and ENT Hospital of Fudan University, Shanghai 200031, China.

Aim: To investigate cap morphology after small-incision lenticule extraction (SMILE) and its effects on intraocular scattering.

Methods: Sixty-five eyes of 33 patients undergoing SMILE were enrolled. In addition to regular evaluation, Fourier-domain optical coherence tomography was used to investigate cap thickness at 1d, 1wk, 1 and 3mo postoperatively. The optical quality including modulation transfer function cutoff frequency, Strehl ratio, Optical Quality Analysis System (OQAS) values, and objective scattering index (OSI), were evaluated using OQAS™.

Results: Cap thickness decreased from 1d to 1wk (<0.001), but remained higher than intended thickness of 120 µm after 3mo (<0.001). Cap thickness in central area was thinner than that of in the paracentral and peripheral areas (<0.0001). Total number of microdistortions decreased from 1d to 3mo (<0.0001). Pearson analysis revealed a weak correlation between OSI and standard deviation of cap thickness at 1d and 1mo, as well as between range of cap thickness and OSI at 1mo. No correlation was found between microdistortion and OSI, but a negative correlation existed between microdistortion and range at 1d and 1moafter surgery.

Conclusion: The corneal cap tends to be more accurate and regular with time lapse. Better cap morphology tends to contribute less intraocular scattering in the eyes undergoing SMILE.
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http://dx.doi.org/10.18240/ijo.2018.03.16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861236PMC
March 2018

Self-assembling Janus dendritic polymer for gene delivery with low cytotoxicity and high gene transfection efficiency.

J Mater Chem B 2016 Oct 26;4(39):6462-6467. Epub 2016 Sep 26.

Department of Pediatrics, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.

Polycations have high DNA condensing ability, low immunogenicity, and great adaptability, which make them promising for gene delivery. However, low transfection efficiency and inevitable toxicity are challenges of cationic polymers. In this study, we prepared a novel Janus dendritic polymer via a one-step Michael addition reaction of branched low-molecular-weight polyethylenimine (PEI, 1800 Da) and dendritic polythioether. The amphiphilic dendritic polymer can self-assemble into stable nanomicelles with high surface charge potential (+91.8 mV). The obtained nanomicelles can be used as a gene delivery vector, which exhibits a higher gene transfection efficiency and much lower cytotoxicity as compared with gold standard PEI (branched, 25 kDa).
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http://dx.doi.org/10.1039/c6tb01891bDOI Listing
October 2016

Refractive Regression and Changes in Central Corneal Thickness Three Years after Laser-Assisted Subepithelial Keratectomy for High Myopia in Eyes with Thin Corneas: A Retrospective Study.

Semin Ophthalmol 2017 1;32(5):631-641. Epub 2016 Jul 1.

a Department of Ophthalmology, Eye and ENT Hospital , Fudan University , Shanghai , PR China.

This retrospective study aimed to explore refractive regression and central corneal thickness (CCT) following laser-assisted subepithelial keratectomy (LASEK) performed for the correction of high myopia in eyes with thin corneas. Forty patients (19 male, 21 female; mean age, 28.5 years) representing 76 treated eyes were included. The mean optical zone was 6.10 ± 0.32 mm, and the mean ablation depth was 121.53 ± 15.48 µm. CCT was significantly greater three years after surgery than at three months after surgery (425.66 ± 15.44 vs. 385.20 ± 12.81, respectively; p<0.001). The mean change in CCT from three months to three years was 40.46 ± 14.02 µm. The SE at three years was greater than that at three months (p<0.001). Although there was refractive regression, these data suggest that LASEK may have utility in the correction of high myopia in eyes with thin corneas.
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http://dx.doi.org/10.3109/08820538.2016.1142579DOI Listing
December 2017

Utilization of High-Fructose Corn Syrup for Biomass Production Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Aurantiochytrium sp. YLH70.

Appl Biochem Biotechnol 2015 Nov 25;177(6):1229-40. Epub 2015 Aug 25.

College of Biological and Environmental Engineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou, 310014, People's Republic of China.

High-fructose corn syrup (HFCS) is an agro-source product and has been the most commonly used substitute for sugar as sweetener in food industry due to its low price and high solution property. In this study, the F55 HFCS, rich in fructose and glucose, was first tested for biomass and docosahexaenoic acid productions as a mixed carbon source by a newly isolated Aurantiochytrium sp.YLH70. After the compositions of the HFCS media were optimized, the results showed that the HFCS with additions of metal ion and vitamin at low concentrations was suitable for biomass and docosahexaenoic acid productions and the metal ion and sea salt had the most significant effects on biomass production. During the 5-l fed-batch fermentation, total HFCS containing 180 g l(-1) reducing sugar was consumed and yields of biomass, lipid, and DHA could reach 78.5, 51, and 20.1 g l(-1), respectively, at 114 h. Meanwhile, the daily productivity and the reducing sugar conversion yield for docosahexaenoic acid were up to 4.23 g l(-1)day(-1) and 0.11 g g(-1). The fatty acid profile of Aurantiochytrium sp.YLH70 showed that 46.4% of total fatty acid was docosahexaenoic acid, suggesting that Aurantiochytrium sp.YLH70 was a promising DHA producer.
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http://dx.doi.org/10.1007/s12010-015-1809-6DOI Listing
November 2015

Biodegradable large compound vesicles with controlled size prepared via the self-assembly of branched polymers in nanodroplet templates.

Chem Commun (Camb) 2014 Sep;50(68):9676-8

CAS Key Lab of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, 230026, Anhui, P. R. China.

Generally, it is very difficult to control the size of large compound vesicles. Here, we introduce a novel method for the preparation of biodegradable large compound vesicles with controlled size and narrow size distribution by using aqueous nanodroplets as templates.
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http://dx.doi.org/10.1039/c4cc02836hDOI Listing
September 2014

Hemokinin-1(4-11)-induced analgesia selectively up-regulates δ-opioid receptor expression in mice.

PLoS One 2014 28;9(2):e90446. Epub 2014 Feb 28.

Zhejiang Provincial People's Hospital, Hangzhou, China ; Institute for Cell-Based Drug Development of Zhejiang Province, Hangzhou, China.

Our previous studies have shown that an active fragment of human tachykinins (hHK-1(4-11)) produced an opioid-independent analgesia after intracerebroventricular (i.c.v.) injection in mice, which has been markedly enhanced by a δ OR antagonist, naltrindole hydrochloride (NTI). In this study, we have further characterized the in vivo analgesia after i.c.v. injection of hHK-1(4-11) in mouse model. Our qRT-PCR results showed that the mRNA levels of several ligands and receptors (e.g. PPT-A, PPT-C, KOR, PDYN and PENK) have not changed significantly. Furthermore, neither transcription nor expression of NK1 receptor, MOR and POMC have changed noticeably. In contrast, both mRNA and protein levels of DOR have been up-regulated significantly, indicating that the enhanced expression of δ opioid receptor negatively modulates the analgesia induced by i.c.v. injection of hHK-1(4-11). Additionally, the combinatorial data from our previous and present experiments strongly suggest that the discriminable distribution sites in the central nervous system between hHK-1(4-11) and r/mHK-1 may be attributed to their discriminable analgesic effects. Altogether, our findings will not only contribute to the understanding of the complicated mechanisms regarding the nociceptive modulation of hemokinin-1 as well as its active fragments at supraspinal level, but may also lead to novel pharmacological interventions.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090446PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938741PMC
June 2015

Macular pigment optical density in aging eye.

Graefes Arch Clin Exp Ophthalmol 2013 Dec 14;251(12):2831-2. Epub 2013 Sep 14.

Department of Ophthalmology, The People's Hospital of Putuo District, Shanghai, China.

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http://dx.doi.org/10.1007/s00417-013-2453-xDOI Listing
December 2013

Hyperopic shift in refraction in adults with aging.

Graefes Arch Clin Exp Ophthalmol 2013 Nov 20;251(11):2661-2. Epub 2013 Jul 20.

Department of Ophthalmology, The People's Hospital of Putuo District, Shanghai, China.

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http://dx.doi.org/10.1007/s00417-013-2425-1DOI Listing
November 2013

Evaluating the association between pathological myopia and SNPs in RASGRF1. ACTC1 and GJD2 genes at chromosome 15q14 and 15q25 in a Chinese population.

Ophthalmic Genet 2015 Mar 8;36(1):1-7. Epub 2013 Jul 8.

Key Laboratory of Myopia, Ministry of Health , Shanghai , China .

Background: This study investigated the association of the 27 SNPs located in RASGRF1. GJD2, and ACTC1 genes with pathological myopia in a Chinese Han population.

Methods: Myopia patients were stratified according to whether they did (n = 274) or did not (n = 131) have myopic macular degeneration (MMD). The SNPbrowser software was used to identify specific SNPs for analysis and minimal allele frequency of >20%, and a pairwise r(2) < 0.85 were genotyped using MALDI-TOF mass spectrometry.

Results: Before controlling for false discovery rate, the frequency of the rs1867315 C/C genotype compared with healthy controls was lower in the myopia group (p = 0.006) and in myopia patients without macular degeneration (p = 0.019). The frequency of the rs670957A/A genotype was also lower in patients without MMD compared with controls (p = 0.045). For rs2070664, the frequency of the A allele was higher in the patients with MMD compared to those without MMD (p = 0.032). After controlling for a false discovery rate of 5%, there was no significant difference in genotype and allele frequencies between these groups.

Conclusion: In this study, there was no association of the analyzed SNPs located in RASGRF1. GJD2, and ACTC1 with pathological myopia, suggesting that SNPs included in our study have no or a limited role in causing pathologic myopia in this Chinese Han population.
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http://dx.doi.org/10.3109/13816810.2013.812737DOI Listing
March 2015

Outer membrane protein OmpW of Escherichia coli is required for resistance to phagocytosis.

Res Microbiol 2013 Oct 25;164(8):848-55. Epub 2013 Jun 25.

School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.

Eight-stranded β-barrel outer membrane proteins can confer bacterial virulence via resistance to host innate defenses. This resistance function of OmpW, which was recently identified as an eight-stranded β-barrel protein, was investigated in this study. Our results demonstrated that upregulation of OmpW correlated with increased bacterial survival during phagocytosis. Bacterial mutants harboring a deletion of ompW exhibited a significantly increased phagocytosis rate. Both observations suggest that the OmpW protein protects bacteria against host phagocytosis. In addition, expression of ompW is regulated by iron, which implies that the resistance provided by OmpW may be an important factor in iron-related infectious diseases. Furthermore, OmpW has been identified as a protective antigen that protects mice against bacterial infection and is therefore a promising target for vaccine development against infectious diseases.
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http://dx.doi.org/10.1016/j.resmic.2013.06.008DOI Listing
October 2013

Association of peripheral hyperopia with axial elongation in high myopia.

Graefes Arch Clin Exp Ophthalmol 2013 Sep 12;251(9):2277. Epub 2013 Feb 12.

Department of Ophthalmology, Putuo Hospital, Shanghai University of Chinese Traditional Medicine, Shanghai, China.

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http://dx.doi.org/10.1007/s00417-013-2277-8DOI Listing
September 2013

Bioreducible and acid-labile poly(amido amine)s for efficient gene delivery.

Int J Nanomedicine 2012 23;7:5819-32. Epub 2012 Nov 23.

Chinese Academy of Sciences, Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.

Intracellular processes, including endosomal escape and intracellular release, are efficiency-determining steps in achieving successful gene delivery. It has been found that the presence of acid-labile units in polymers can facilitate endosomal escape and that the presence of reducible units in polymers can lead to intracellular release. In this study, poly(amido amine)s with both bioreducible and acid-labile properties were synthesized to improve gene delivery compared with single-responsive carriers. Transfection and cytotoxicity were evaluated in three cell lines. The complexes of DNA with dual-responsive polymers showed higher gene transfection efficiency than single-responsive polymers and polyethylenimine. At the same time, these polymers were tens of times less cytotoxic than polyethylenimine. Therefore, a polymer that is both reducible and acid-labile is a promising material for efficient and biocompatible gene delivery.
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http://dx.doi.org/10.2147/IJN.S37334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3511192PMC
April 2013

Synthesis of bioreducible polycations with controlled topologies.

Methods Mol Biol 2013 ;948:121-32

CAS Key Lab of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, People's Republic of China.

Bioreducible polycations, which possess disulfide linkages in the backbone, have appeared as promising gene delivery carriers due to their high stability in extracellular physiological condition and bioreduction-triggered release of genetic materials, as well as reduced cytotoxicity because intracellular cytosol is a reducing environment containing high level of reducing molecules such as glutathione. Here, we describe the syntheses of bioreducible polycations, and the methods for control over their topology are also presented.
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http://dx.doi.org/10.1007/978-1-62703-140-0_9DOI Listing
March 2013

Polycyclic aromatic hydrocarbons in animal-based foods from Shanghai: bioaccessibility and dietary exposure.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2012 5;29(9):1465-74. Epub 2012 Jul 5.

Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai, P R China.

A total of 175 samples of 18 types of food were collected from markets in Shanghai, China, and the concentrations and bioaccessibility of 15 priority-controlled polycyclic aromatic hydrocarbons (PAHs) in these samples were determined. The mean concentrations of PAHs varied between 2.4 and 47.1 ng g(-1) wet weight, with the highest being observed in snail and lowest in chicken. The concentrations were lower than the maximum levels of PAH allowed for food per EU regulations. Among the PAHs measured, phenanthrene was the predominant one. Most of the PAHs originated from pyrogenic sources, analysed using molecular indices. The mean bioaccessibility of PAHs varied from 29.0% to 61.2% as measured by simulating the human gastrointestinal digestion process. Linear relationships between the bioaccessibility and lipid contents were observed for most PAH congeners. The daily intake of PAHs by an average Shanghai resident was 848 ng day(-1) and decreased to 297 ng day(-1) when the bioaccessibility of PAHs were considered, demonstrating that most intake might have been overestimated. According to the potency equivalent concentrations and screening values of PAHs, consumption of snail and clam, especially snail, should be limited.
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http://dx.doi.org/10.1080/19440049.2012.694121DOI Listing
December 2012

Polycyclic aromatic hydrocarbons and organochlorine pesticides in fish from Taihu Lake: their levels, sources, and biomagnification.

Ecotoxicol Environ Saf 2012 Aug 5;82:63-70. Epub 2012 Jun 5.

Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, PR China.

The investigation of biomagnification of polycyclic aromatic hydrocarbons (PAHs) and endosulfan, an organochlorine pesticide (OCP) and a new persistent organic pollutant, has been limited in freshwater food chains. The objective of the present study was to investigate the levels with focus on the sources and biomagnification of PAHs and OCPs in fish from Taihu Lake, China. In 193 samples of 24 species investigated, the concentrations ranged from 289 to 9 500 ng/g lipid weight (lw) for PAHs, and from 121 to 904 ng/g lw for OCPs, indicating that the fish in the lake was moderately contaminated. The PAHs mainly originated from both unburned petroleum and combustion of fossil fuels, and the OCPs from aged residues. It was unlikely that most of the PAHs and OCPs were biodiluted through the food chain because their trophic magnification factors were higher than one nevertheless the P-values >0.05. Aldrin, dieldrin, p,p'-DDE, p,p'-DDD, and endosulfan sulfate were significantly biomagnified through the food chain.
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http://dx.doi.org/10.1016/j.ecoenv.2012.05.010DOI Listing
August 2012

Tissue-specific distribution of fatty acids, polychlorinated biphenyls and polybrominated diphenyl ethers in fish from Taihu Lake, China, and the benefit-risk assessment of their co-ingestion.

Food Chem Toxicol 2012 Aug 1;50(8):2837-44. Epub 2012 Jun 1.

Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, PR China.

The fish tissues from four species collected from Taihu Lake, China, were analyzed including dorsal, ventral, and tail muscles, heart, liver, and kidney. The highest and lowest concentrations of fatty acids were respectively observed in livers and muscles. There were significant intraspecies and interspecies differences in the compositions of most fatty acids among muscle, heart, liver, and kidney. All the tissues were generally beneficial for consumption considering fatty acids. People mainly consume the muscle. Hence, the benefits from two polyunsaturated fatty acids, i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and risks from PCBs and PBDEs via fish consumption were evaluated by calculating the benefit-risk quotient (BFQ) for the intake of fish muscle containing EPA+DHA vs. PCBs or PBDEs. The BFQ values considering carcinogenic and noncarcinogenic effects for PCBs were ∼3000 and 10 times higher than those of PBDEs via fish consumption to achieve the recommended EPA+DHA intake of 250 mg d(-1), respectively. The results also suggested that the risk consuming the dorsal muscle was generally lower than the ventral and tail muscles.
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http://dx.doi.org/10.1016/j.fct.2012.05.043DOI Listing
August 2012

Bioreducible nanogels/microgels easily prepared via temperature induced self-assembly and self-crosslinking.

Chem Commun (Camb) 2012 Jun 24;48(45):5623-5. Epub 2012 Apr 24.

CAS Key Lab of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, 230026, Anhui, PR China.

A facile temperature induced self-assembly and self-crosslinking method has been developed for preparing bioreducible nanogels/microgels without need of any stabilizer, catalyst or additional crosslinking agent. The size of formed nanogels/microgels can be easily tuned via the polymer concentration.
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http://dx.doi.org/10.1039/c2cc30908dDOI Listing
June 2012

Biocompatible zwitterionic sulfobetaine copolymer-coated mesoporous silica nanoparticles for temperature-responsive drug release.

Macromol Rapid Commun 2012 May 5;33(9):811-8. Epub 2012 Apr 5.

CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, P R China.

A novel nanocontainer, which could regulate the release of payloads, has been successfully fabricated by attaching zwitterionic sulfobetaine copolymer onto the mesoporous silica nanoparticles (MSNs). RAFT polymerization is employed to prepare the hybrid poly(2-(dimethylamino)ethyl methacrylate)-coated MSNs (MSN-PDMAEMA). Subsequently, the tertiary amine groups in PDMAEMA are quaternized with 1,3-propanesultone to get poly(DMAEMA-co-3-dimethyl(methacryloyloxyethyl)ammonium propanesulfonate)-coated MSNs [MSN-Poly(DMAEMA-co-DMAPS)]. The zwitterionic PDMAPS component endows the nanocarrier with biocompatibility, and the PDMAEMA component makes the copolymer shell temperature-responsive. Controlled release of loaded rhodamine B has been achieved in the saline solutions.
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http://dx.doi.org/10.1002/marc.201100876DOI Listing
May 2012

Levels and distribution of synthetic musks and polycyclic aromatic hydrocarbons in sludge collected from Guangdong Province.

J Environ Sci Health A Tox Hazard Subst Environ Eng 2012 ;47(3):389-97

State Key Laboratory of Organic Geochemistry, Guangdong Key Laboratory of Environment and Resources, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China.

The levels and distribution of six polycyclic musks, three nitromusks and 15 polycyclic aromatic hydrocarbons (PAHs) were investigated in sludge collected from 19 municipal wastewater treatment plants (WWTPs) in six cities in Guangdong Province, China. PAHs were detected in all of the sludge samples, and the levels of the total 15 PAHs ranged from 177.2-4421.8 μg/kg dry weight (dw). Four polycyclic musks, 4-acetyl-1,1-dimethyl-6-tert-butylindan (ADBI), 6-acetyl-1,1,2,3,3,5-hexamethylindan (AHMI), 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta (g) -2-benzopyran (HHCB) and 7-acetyl -1,1,3,4,4,6-hexamethyl-1,2,3,4-tetrahydro naphthalene (AHTN), were found in these samples. The total concentrations of polycyclic musks varied from 794.4-12960.3 μg/kg dw, with HHCB and AHTN being the main components. Of the three nitromusks, 2,6-dinitro-3-methoxy-4-tert- butyl - toluene (MA) was only found in one sludge sample at the limit of detection (LOD) level, while 1-tert-butyl-3,5-dimethyl-2,4,6-trinitrobenzene (Musk xylene, MX) and 4-acetyl-1-tert-butyl-3,5-dimethyl-2,6-dinitrobenzene (Musk ketone, MK) were found at levels ranging from the LOD to 65.8 μg/kg dw and LOD to 172.7 μg/kg dw, respectively, in most of the sludge samples. The PAHs, polycyclic musks and nitro musks were also shown to have various distribution patterns, possibly due to their different wastewater sources and wastewater treatment technology.
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http://dx.doi.org/10.1080/10934529.2012.646099DOI Listing
May 2012

[Method of ecological risk assessment for risk pollutants under short-term and high dose exposure in water pollution accident].

Huan Jing Ke Xue 2011 Nov;32(11):3240-6

Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.

In recent years, water pollution accidents resulting in acute aquatic ecological risk and security issues become a research focus. However, in our country, the surface water quality standards and drinking water health standards were used to determine the safety of waters or not in pollution incidents due to lacking safety effect threshold or risk value for protection of aquatic life. In foreign countries, although predicted no effect concentration (PNEC) or risk value (R) of pollutants were provided for protection of aquatic organisms, the PNECs or risk values were derived based on long-term exposure toxicity data NOECs (no observed effect concentrations) and lack of short-term exposure risk or threshold values. For the short-term and high dose exposure in pollution incident, ecological risk assessment methods were discussed according to the procedures of the conventional ecological risk assessment and the water quality criteria establishment of the U.S. EPA for the protection of aquatic organisms in short-term exposure, and had a case study. At the same time, we provide some suggestions for the establishment of ecological risk assessment system in water pollution incidents.
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November 2011

Polybrominated diphenyl ethers and polychlorinated biphenyls in freshwater fish from Taihu Lake, China: their levels and the factors that influence biomagnification.

Environ Toxicol Chem 2012 Mar 6;31(3):542-9. Epub 2012 Feb 6.

Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai, P.R. China.

Polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs), two types of persistent organic pollutants that have been used widely, can be released into the environment and accumulate in organisms. To obtain a better understanding of the biomagnification of PBDEs and PCBs in fish, as well as the influences on biomagnification by fish size and physical properties of PBDEs and PCBs, a total of 200 samples of 24 fish species were collected and analyzed from Taihu Lake, the second largest freshwater lake in China. The concentrations of PBDEs and PCBs ranged from 8.59 to 74.28 ng/g lipid weight (lw) and from 10.30 to 165.20 ng/g lw, respectively. Trophic magnification factors (TMFs) were used to estimate the PBDE and PCB biomagnification potentials. The TMF values of PBDEs and PCBs ranged from 0.78 to 2.95 and from 0.92 to 2.60, respectively. Most of the TMFs were greater than 1, indicating that these contaminants were biomagnified in food chains. Fish size might influence the biomagnification evaluation, because different sized fish had different lipid content, leading to different lipid-based concentrations of PBDEs and PCBs. Parabolic relationships were observed between the TMFs and logK(OW) , as well as between the TMFs and the molecular volumes of PBDE and PCB congeners. The congeners with logK(OW) values of approximately 7 or molecular volumes of approximately 8 × 10⁻⁵  nm³ had the greatest biomagnification potentials. Compared to molecular weight, molecular volume seems to be the better standard for analyzing the influence of molecular size on biomagnification.
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http://dx.doi.org/10.1002/etc.1722DOI Listing
March 2012

Concentrations and seasonal variations of polybrominated diphenyl ethers (PBDEs) in in- and out-house dust and human daily intake via dust ingestion corrected with bioaccessibility of PBDEs.

Environ Int 2012 Jul 22;42:124-31. Epub 2011 Jun 22.

Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, PR China.

The objective of this study was to investigate the concentrations, seasonal variations, bioaccessibility, and associated human daily intake of polybrominated diphenyl ethers (PBDEs) in in- and out-house dust collected in Shanghai, China. The PBDE concentrations varied from 131.6 to 3,886.7 ng g(-1) (with an average of 948.2 ng g(-1)) in in-house dust and from 8.7 to 3,116.3 ng g(-1) (with an average of 290.8 ng g(-1)) in out-house dust during four seasons. The PBDE concentrations in the autumn were the lowest for both in- and out-house dust. Among the detected PBDEs, BDE209 was the predominant congener, accounting for more than 80% of the total PBDE amounts. The bioaccessibility of PBDEs, measured using a simulation system of human gastrointestinal tract, was determined as 14.2-66.4% depending on individual PBDE congeners and showed significant negative correlations with organic matter in dust. After corrected with the bioaccessibility of PBDEs, the human daily intake of PBDEs via dust ingestion was calculated to be 0.4-21.4 and 4.3-40.6 ng day(-1) for an average adult and child in Shanghai, respectively. The values were much lower than most estimates in the literature, in which the bioaccessibility of PBDEs were not taken into account, suggesting that the intake of PBDEs may have been overestimated.
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http://dx.doi.org/10.1016/j.envint.2011.05.012DOI Listing
July 2012

Increased urinary 8-hydroxy-2'-deoxyguanosine levels in workers exposed to di-(2-ethylhexyl) phthalate in a waste plastic recycling site in China.

Environ Sci Pollut Res Int 2011 Jul 6;18(6):987-96. Epub 2011 Feb 6.

Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, 13 Hongkong Road, Wuhan 430030, People's Republic of China.

Background: Di-(2-ethylhexyl) phthalate (DEHP) is a common plasticizer used in industrial and diverse consumer products. Animal studies indicate DEHP caused developmental, reproductive, and hepatic toxicities. However, human studies of the potential effects of DEHP are limited.

Methods: The exposed site with a history of over 20 years of waste plastic recycling was located in Hunan Province, China. The reference site without known DEHP pollution source was about 50 km far away from the exposed site. In this study, 181 workers working in plastic waste recycling and 160 gender-age matched farmers were recruited. DEHP concentrations in water and cultivated soil samples, serum thyroid-stimulating hormone, malondialdehyde (MDA), superoxide dismutase (SOD), urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), and micronuclei frequency in human capillary blood lymphocytes were analyzed.

Results: Mean levels of DEHP were greater in environment at the recycling site than at reference site (industry wastewater for the exposed: 42.43 μg/l; well water: 14.20 vs. 0.79 μg/l, pond water: 135.68 vs. 0.37 μg/l, cultivated soil: 13.07 vs. 0.81 mg/kg, p < 0.05 for all). The workers had higher median levels of MDA (3.80 vs. 3.14 nmol/ml) and urinary 8-OHdG (340.37 vs. 268.18 μmol/mol creatinine) and decreased SOD activities (112.15 vs. 123.82 U/ml) than the reference group (p < 0.01 for all). Multivariate analysis revealed that the history of working in waste plastic recycling was an independent risk factor for the increased urinary 8-OHdG levels in the male workers (p < 0.01).

Conclusions: The occupational DEHP exposure might contribute to oxidative deoxyribonucleic acid damage in the male workers.
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http://dx.doi.org/10.1007/s11356-010-0420-1DOI Listing
July 2011