Publications by authors named "Zhi-Qiang Chen"

152 Publications

Publisher Correction: Leveraging breeding programs and genomic data in Norway spruce (Picea abies L. Karst) for GWAS analysis.

Genome Biol 2021 Jul 15;22(1):210. Epub 2021 Jul 15.

Umeå Plant Science Centre, Department Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90183, Umeå, Sweden.

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http://dx.doi.org/10.1186/s13059-021-02421-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281665PMC
July 2021

Leveraging breeding programs and genomic data in Norway spruce (Picea abies L. Karst) for GWAS analysis.

Genome Biol 2021 Jun 13;22(1):179. Epub 2021 Jun 13.

Umeå Plant Science Centre, Department Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90183, Umeå, Sweden.

Background: Genome-wide association studies (GWAS) identify loci underlying the variation of complex traits. One of the main limitations of GWAS is the availability of reliable phenotypic data, particularly for long-lived tree species. Although an extensive amount of phenotypic data already exists in breeding programs, accounting for its high heterogeneity is a great challenge. We combine spatial and factor-analytics analyses to standardize the heterogeneous data from 120 field experiments of 483,424 progenies of Norway spruce to implement the largest reported GWAS for trees using 134 605 SNPs from exome sequencing of 5056 parental trees.

Results: We identify 55 novel quantitative trait loci (QTLs) that are associated with phenotypic variation. The largest number of QTLs is associated with the budburst stage, followed by diameter at breast height, wood quality, and frost damage. Two QTLs with the largest effect have a pleiotropic effect for budburst stage, frost damage, and diameter and are associated with MAP3K genes. Genotype data called from exome capture, recently developed SNP array and gene expression data indirectly support this discovery.

Conclusion: Several important QTLs associated with growth and frost damage have been verified in several southern and northern progeny plantations, indicating that these loci can be used in QTL-assisted genomic selection. Our study also demonstrates that existing heterogeneous phenotypic data from breeding programs, collected over several decades, is an important source for GWAS and that such integration into GWAS should be a major area of inquiry in the future.
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http://dx.doi.org/10.1186/s13059-021-02392-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201819PMC
June 2021

RECK expression is associated with angiogenesis and immunogenic Tumor Microenvironment in Hepatocellular Carcinoma, and is a prognostic factor for better survival.

J Cancer 2021 5;12(13):3827-3840. Epub 2021 May 5.

Department of general surgery, Qilu Hospital, Shandong University, Jinan 250012, China.

Angiogenesis and immunosuppression have been described as closely related processes that can occur in parallel. As an inhibitor of matrix metalloproteinase, whether the level of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) in hepatocellular carcinoma (HCC) reflects a link between angiogenesis and immunosuppression is still unknown. We analyzed RNA expression, immune infiltration and survival of HCC from The Cancer Genome Atlas databases. Immune scores and stromal scores were calculated based on the ESTIMATE algorithm to quantify the immune and stromal components in HCC. The association between RECK and clinicopathological features was further investigated by immunohistochemistry on tissue microarray. We found that the prognosis of patients with high RECK expression was significantly better than that of patients with low RECK expression. High RECK expression was associated with high ESTIMATE Score, recruitment of more tumor-infiltrating lymphocytes, low tumor purity, and high PD-L1 expression. In addition, positive RECK expression was associated with a lower incidence of vascular invasion and recurrence, a lower level of alpha fetoprotein (AFP) and microvessel density and a better tumor differentiation. Multivariate analyses revealed that reduced RECK expression was an independent prognostic factor for recurrence and poor prognosis. In conclusion, high RECK expression reflects an immunogenic and hypovascularity status in HCC. RECK is a promising prognostic marker for survival of HCC and may act as a complementary indicator for patients to receive anti-angiogenic therapy or immunotherapy.
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http://dx.doi.org/10.7150/jca.56167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176247PMC
May 2021

Organisational innovation climate and innovation behaviour among nurses in China: A mediation model of psychological empowerment.

J Nurs Manag 2021 May 22. Epub 2021 May 22.

Department of Operating Room, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong Province, China.

Aim: This study aimed to explore the effect of organisational innovation climate on nurse innovation behaviour and the mediating role of psychological empowerment.

Background: Encouraging nurses to generate more innovative behaviours has become an important development direction for improving the quality of nursing services.

Method: We employed a self-report questionnaire to collect data in Jinan City, China. A total of 2018 valid surveys were obtained. Hierarchical multiple regression model analysis was conducted to test the study hypothesis.

Result: The mean values of innovation behaviour and organisational innovation climate were 35.29 and 83.30, respectively. Psychological empowerment was found to have partially mediating effect on the relationship between organisational innovation climate and innovation behaviour.

Conclusion: Organisational innovation climate has significant impact on innovation behaviour, and it can indirectly affect innovation behaviour via the mediating role of psychological empowerment.

Implications For Nursing Management: Nursing managers should enhance innovation climate through formal rules, procedures and training activities. They can establish resource guarantee system and information sharing platform, and strengthen work autonomy for nurses to improve their psychological empowerment.
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http://dx.doi.org/10.1111/jonm.13381DOI Listing
May 2021

Advertisement calls of the lesser spiny frog Quasipaa exilispinosa (Liu and Hu, 1975) (Anura: Dicroglossidae).

Zootaxa 2021 Feb 9;4926(3):zootaxa.4926.3.9. Epub 2021 Feb 9.

Laboratory of Amphibian Diversity Investigation, College of Ecology, Lishui University, Lishui 323000, Zhejiang, China.

There are currently only eleven species of Quasipaa (Anura: Dicroglossidae) distributed in southern and southwestern China to central Vietnam, southeastern Thailand, and southwestern Cambodia and presumably also in Laos (Frost 2020). Eight species of Quasipaa are currently known in China: Q. boulengeri (Günther 1889), Q. courtoisi (Angel 1922), Q. exilispinosa (Liu Hu 1975), Q. jiulongensis (Huang Liu 1985), Q. shini (Ahl 1930), Q. spinosa (David 1875), Q. yei (Chen, Qu, and Jiang 2002), and Q. verrucospinosa (Bourret 1937). The first seven species are endemic to China (AmphibiaChina 2020). These species have similar morphological traits and are capable of introgressive hybridization between the closely related species of this genus(Zhang et al. 2018). Despite the detailed acoustic analysis that is available to identify a variety of species and is beneficial to the study of anuran taxonomy (e.g., Microhyla species; Chen et al. 2020), advertisement calls have only been reported in detail for Q. spinosa (Yu Zheng 2009; Chen et al. 2012; Shen et al. 2015) and Q. shini (Kong et al. 2016), whereas the call for Q. exilispinosa was briefly described from observations in captivity (Voitel 2000).
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http://dx.doi.org/10.11646/zootaxa.4926.3.9DOI Listing
February 2021

A rare type of choledochal cysts of Todani type IV-B with typical pancreaticobiliary maljunction.

Hepatobiliary Pancreat Dis Int 2021 Mar 9. Epub 2021 Mar 9.

Department of Hepatobiliary Surgery, General Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, China. Electronic address:

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http://dx.doi.org/10.1016/j.hbpd.2021.03.002DOI Listing
March 2021

Xiangbinfang granules enhance gastric antrum motility intramuscular interstitial cells of Cajal in mice.

World J Gastroenterol 2021 Feb;27(7):576-591

The Research Team of TCM Applications of Perioperative, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, Guangdong Province, China.

Background: Interdigestive migrating motor complexes (MMC) produce periodic contractions in the gastrointestinal tract, but the exact mechanism of action still remains unclear. Intramuscular interstitial cells of Cajal (ICC-IM) participate in gastrointestinal hormone and neuromodulation, but the correlation between ICC-IM and MMC is also unclear. We found that xiangbinfang granules (XBF) mediated the phase III contraction of MMC. Here, the effects of XBF on gastric antrum motility in mice and the effects of ICC-IM on gastric antrum MMC are reported.

Aim: To observe the effects of ICC-IM on gastric antrum motility and to establish the mechanism of XBF in promoting gastric antrum motility.

Methods: The density of c-kit-positive ICC myenteric plexus (ICC-MP) and ICC-IM in the antral muscularis of and wild-type (WT) mice was examined by confocal microscopy. The effects of XBF on gastric antrum slow waves in and WT mice were recorded by intracellular amplification recording. Micro-strain-gauge force transducers were implanted into the gastric antrum to monitor the MMC and the effect of XBF on gastric antrum motility in conscious and WT mice.

Results: In the gastric antrum of mice, c-kit immunoreactivity was significantly reduced, and no ICC-IM network was observed. Spontaneous rhythmic slow waves also appeared in the antrum of mice, but the amplitude of the antrum slow wave decreased significantly in mice (22.62 ± 2.23 mV 2.92 ± 0.52 mV, < 0.0001). MMCs were found in 7 of the 8 WT mice but no complete MMC cycle was found in mice. The contractile frequency and amplitude index of the gastric antrum were significantly increased in conscious WT compared to mice (frequency, 3.53 ± 0.18 cpm 1.28 ± 0.12 cpm; amplitude index, 23014.26 ± 1798.65 mV·20 min 3782.16 ± 407.13 mV·20 min; < 0.0001). XBF depolarized smooth muscle cells of the gastric antrum in WT and mice in a dose-dependent manner. Similarly, the gastric antrum motility in WT mice was significantly increased after treatment with XBF 5 mg ( < 0.05). Atropine (0.1 mg/kg) blocked the enhancement of XBF in WT and mice completely, while tetrodotoxin (0.05 mg/kg) partially inhibited the enhancement by XBF.

Conclusion: ICC-IM participates in the regulation of gastric antrum MMC in mice. XBF induces MMC III-like contractions that enhance gastric antrum motility ICC-IM in mice.
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http://dx.doi.org/10.3748/wjg.v27.i7.576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901053PMC
February 2021

Next-generation sequencing yields the complete mitochondrial genome of (Anura: Microhylidae).

Mitochondrial DNA B Resour 2020 Oct 9;5(3):3529-3530. Epub 2020 Oct 9.

Laboratory of Amphibian Diversity Investigation, College of Ecology, Lishui University, Lishui, Zhejiang, China.

The complete mitochondrial genome (mtDNA) of (Anura: Microhylidae) was sequenced and annotated. The length of mtDNA sequences of was 16,721 bp, and encoded 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and a control region. The overall nucleotide composition of this genome was 29.1% A, 24.5% C, 14.5% G, 31.9% T, with a total A + T content of 61%. Phylogenetic analysis using Bayesian Inference (BI) method revealed that was closely related with other 8 species from the genus . The mtDNA dataset could be utilized for studying the molecular ecology and population genetics of Microhylid frogs.
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http://dx.doi.org/10.1080/23802359.2020.1827068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782835PMC
October 2020

Effects of hypoxia-inducible factor-1α and hypoxia-inducible factor-2α overexpression on hepatocellular carcinoma survival: A systematic review with meta-analysis.

J Gastroenterol Hepatol 2021 Jun 26;36(6):1487-1496. Epub 2021 Jan 26.

Department of General Surgery, Qilu Hospital, Shandong University, Jinan, China.

Background And Aim: The role of hypoxia-inducible factor-1α (HIF-1α) and hypoxia-inducible factor-2α (HIF-2α) has been implicated in the clinical prognosis of hepatocellular carcinoma (HCC), but the results remain controversial. We aim to investigate the association of HIF-1α and HIF-2α overexpression with the prognosis and clinicopathological features of HCC.

Methods: A systematic search was conducted in PubMed, Embase, Scopus, Web of Science, and Cochrane Library until June 20, 2020. Meta-analysis was conducted to generate combined HRs with 95% confidence intervals (CI) for overall survival (OS) and disease-free survival (DFS). Odds ratios (ORs) with 95% CI were also derived by fixed or random effect model.

Results: Twenty-two studies involving 3238 patients were included. Combined data suggested that overexpression of HIF-1α in HCC was not only correlated with poorer OS [HR = 1.75 (95% CI: 1.53-2.00)] and DFS [HR = 1.64 (95% CI: 1.34-2.00)] but was also positively associated with vascular invasion [OR = 1.83 (95% CI: 1.36-2.48)], tumor size [OR = 1.36 (95% CI: 1.12-1.66)], and tumor number [1.74 (95% CI: 1.34-2.25)]. In contrast, HIF-2α overexpression was not associated with the prognosis and clinicopathological features of HCC.

Conclusion: Our data provided compelling evidence of a worse prognosis of HCC in HIF-1α overexpression patients but not HIF-2α overexpression ones.
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http://dx.doi.org/10.1111/jgh.15395DOI Listing
June 2021

Survival outcomes following laparoscopic vs open surgery for non-metastatic rectal cancer: a two-center cohort study with propensity score matching.

Gastroenterol Rep (Oxf) 2020 Aug 19;8(4):319-325. Epub 2020 Aug 19.

Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P. R. China.

Background?>: Laparoscopic surgery for rectal cancer is commonly performed in China. However, compared with open surgery, the effectiveness of laparoscopic surgery, especially the long-term survival, has not been sufficiently proved.

Methods?>: Data of eligible patients with non-metastatic rectal cancer at Nanfang Hospital of Southern Medical University and Guangdong Provincial Hospital of Chinese Medicine between 2012 and 2014 were retrospectively reviewed. Long-term survival outcomes and short-term surgical safety were analysed with propensity score matching between groups.

Results: Of 430 cases collated from two institutes, 103 matched pairs were analysed after propensity score matching. The estimated blood loss during laparoscopic surgery was significantly less than that during open surgery (=0.019) and the operative time and hospital stay were shorter in the laparoscopic group (both <0.001). The post-operative complications rate was 9.7% in the laparoscopic group and 10.7% in the open group (=0.818). No significant difference was observed between the laparoscopic group and the open group in the 5-year overall survival rate (75.7% vs 80.6%, =0.346), 5-year relapse-free survival rate (74.8% vs 76.7%, =0.527), or 5-year cancer-specific survival rate (79.6% vs 87.4%, =0.219). An elevated carcinoembryonic antigen, <12 harvested lymph nodes, and perineural invasion were independent prognostic factors affecting overall survival and relapse-free survival.

Conclusions?>: Our findings suggest that open surgery should still be the priority recommendation, but laparoscopic surgery is also an acceptable treatment for non-metastatic rectal cancer.
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http://dx.doi.org/10.1093/gastro/goaa046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7434561PMC
August 2020

Rapid rehabilitation technique with integrated traditional Chinese and Western medicine promotes postoperative gastrointestinal function recovery.

World J Gastroenterol 2020 Jun;26(23):3271-3282

The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong province, China.

Background: During the perioperative period, the characteristic therapy of traditional Chinese medicine is effective in improving postoperative rehabilitation. In large-scale hospitals practicing traditional Chinese medicine, there is accumulating experience related to the promotion of fast recovery in the perioperative period.

Aim: To evaluate the efficacy and safety of Yikou-Sizi powder hot compress on Shenque acupuncture point combined with rapid rehabilitation technique.

Methods: This prospective, multicenter, randomized, controlled study included two groups: Treatment group and control group. The patients in the treatment group and control group received Yikou-Sizi powder hot compress on Shenque acupuncture point combined with rapid rehabilitation technique and routine treatment, respectively. Clinical observation regarding postoperative recovery of gastrointestinal function was performed, including the times to first passage of flatus, first defecation, and first normal bowel sounds. The comparison between groups was conducted through descriptive analysis, , , and rank-sum tests.

Results: There was a statistically significant difference in the time to postoperative first defecation between the treatment and control group (87.16 ± 32.09 109.79 ± 40.25 h, respectively; < 0.05). Similarly, the time to initial recovery of bowel sounds in the treatment group was significantly shorter than that in the control group (61.17 ± 26.75 79.19 ± 33.35 h, respectively; < 0.05). However, there was no statistically significant difference in the time to initial exhaust between the treatment and control groups (51.54 ± 23.66 62.24 ± 25.95 h, respectively; > 0.05). The hospitalization expenses for the two groups of patients were 62283.45 ± 12413.90 and 62059.42 ± 11350.51 yuan, respectively. Although the cost of hospitalization was decreased in the control group, the difference was not statistically significant ( > 0.05). This clinical trial was safe without reports of any adverse reaction or event.

Conclusion: The rapid rehabilitation technique with integrated traditional Chinese and Western medicine promotes the recovery of postoperative gastrointestinal function and is significantly better than standard approach for patients after colorectal surgery.
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http://dx.doi.org/10.3748/wjg.v26.i23.3271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336322PMC
June 2020

[The improvement effects of Yiqi Huashi Tongluo Formula on oxidative stress and renal fibrosis of experimental CRF rats].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2020 Jan;36(1):67-72

Hebei University of Chinese Medicine.

Objective: Effects of Yiqi Huashi Tongluo Formula on oxidative stress and renal fibrosis of residual kidney were investigated in five/sixth nephrectomy rats.

Methods: The rat model of chronic renal failure after nephrectomy was established by Platt method. Two weeks after the operation, the rats were randomly divided into model group, Yiqi Huashi Tongluo Formula (YHT) group, benazepril online (BH) group and sham group, with 8 rats in each group. Treatment was initiated once a day for 12 weeks after successful modeling. Animals were treated once a day with intragastric administration for 12 weeks. (Aqueous solution of free decoction granules in YHT group was 0.276 g/100 g·d. BH group benazepril hydrochloride tablet aqueous solution 0.09 mg/100 g·d gavage; sham group and model group were gavage with 1 ml/100 g normal saline). Urine was collected with a metabolic cage at the end of the 12th week, and urine protein content was detected for 24 hours. The rats were then anesthetized to extract blood from the abdominal aorta and the kidneys. The pathological changes of left kidney were observed by HE staining and Masson staining. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in kidney homogenate were determined by colorimetry. Western blot assay was used to detect the expressions of nuclear factor-erythroid 2-related factor 2 (Nrf2), kelch-like ech-associated protein-1 (Keap1), NADPH oxidase 4 (Nox4), transforming growth factor-binding 1(TGF-β1), type I collagen (Collagen1) and Nrf2 in the nucleus in renal tissue.

Results: Compared with sham group, model group rats had severe glomerular injury and obvious fibrosis. Levels of Scr, BUN, MDA and 24-hour urine protein excretion, protein expressions of Keap1, Nox4, TGF-β1 and Collagen1 were significantly increased (P<0.01), while SOD activity and Nrf2 expression were significantly decreased (P<0.01).Compared with the model group, the degree of glomerular lesion was reduced and fibrosis was less after YHT or BH intervention, and the levels of Scr, BUN, MDA, 24-hour urine protein excretion, protein expressions of Keap1, Nox4, TGF-β1 and Collagen1 were significantly decreased (P<0.01), while SOD activity and Nrf2 expression were significantly increased (P<0.01).

Conclusion: Through affecting the Nrf2/Keap1 signaling pathway and down-regulating the expression of TGF-β1 protein, Yiqi Huashi Tongluo Formula improved the oxidative stress damage and fibrosis degree of residual kidney in the model rats with renal failure.
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http://dx.doi.org/10.12047/j.cjap.5928.2020.015DOI Listing
January 2020

Red blood cell distribution width is associated with mortality risk in patients with acute respiratory distress syndrome based on the Berlin definition: A propensity score matched cohort study.

Heart Lung 2020 Sep - Oct;49(5):641-645. Epub 2020 May 17.

Department of Intensive Care, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, 325000, China. Electronic address:

Background: Acute respiratory distress syndrome (ARDS) is a severe inflammatory disorder of the lungs and is associated with oxidative damage. However, red blood cell distribution width (RDW), as an indicator of body response to inflammation and oxidative stress, has not been studied for its relationship with ARDS as diagnosed by the Berlin definition.

Objectives: To examine the value of RDW in predicting the prognosis of in patients with ARDS.

Methods: This is a retrospective study based on the Medical Information Mart for Intensive Care III (MIMIC-III) database. Berlin-defined ARDS patients using mechanical ventilation for more than 48 hours were selected using structured query language. The primary statistical methods were propensity score matching and sensitivity analysis, including an inverse probability weighting model to ensure the robustness of our findings.

Results: A total of 529 intensive care unit (ICU) patients with ARDS according to the Berlin definition were enrolled in the study. The adjusted OR showed an adverse effect between the higher RDW group and 30-day mortality [OR 2.33, 95% CI (1.15-4.75), P=0.019]. However, we found that length of ICU stay was not related to RDW (P=0.167), and in the anaemia group, RDW was poorly predictive of 30-day mortality (P=0.307).

Conclusion: In unselected ARDS patients, higher RDW was associated with higher 30-day mortality rate. Further investigation is required to validate this relationship with prospectively collected data.
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http://dx.doi.org/10.1016/j.hrtlng.2020.04.008DOI Listing
March 2021

[Ki67 in the prognosis of prostate cancer].

Zhonghua Nan Ke Xue 2019 Dec;25(12):1088-1092

Department of Radiology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China.

Objective: To investigate the influencing factors and the role of Ki67 in the prognosis of PCa.

Methods: We collected the clinical and follow-up data on 141 cases of PCa pathologically confirmed in the General Hospital of Ningxia Medical University from January 2008 to December 2014. We analyzed the factors possibly influencing the prognosis of PCa, including age, PSA level, Gleason score, clinical stage and Ki67, using the Kaplan-Meier method for univariate analysis, the Cox proportional hazards regression model for multivariate analysis and the multivariate Cox proportional hazards regression model for multiplicative interaction analysis.

Results: Kaplan-Meier univariate analysis indicated that the main factors influencing the prognosis of PCa included Ki67 (χ2 = 38.507, P < 0.01), clinical stage (χ2 = 59.486, P < 0.01) and Gleason score (χ2 = 9.062, P < 0.05); Cox multivariate analysis showed Ki67 (HR = 1.88, P < 0.01) and clinical stage (HR = 1.92, P < 0.01) to be the risk factors; and interaction analysis demonstrated that Ki67 was not correlated with either the clinical stage or Gleason score.

Conclusions: Ki67 is a risk factor in the prognosis of PCa.
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December 2019

The advertisement call of the moustache toad Leptobrachium liui Pope, 1947 (Anura: Megophryidae) from eastern China.

Zootaxa 2020 Feb 14;4732(4):zootaxa.4732.4.8. Epub 2020 Feb 14.

Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, Jiangsu, China Laboratory of Amphibian Diversity Investigation,, College of Ecology, Lishui University, Lishui 323000, Zhejiang, China.

The Leptobrachium genus is currently composed of 36 species distributed in Southern China, India, islands of the Sunda Shelf, and the Philippines (Frost 2019). In China, 11 species of the genus Leptobrachium are currently known (AmphibiaChina 2019), of which, the following nine are Chinese endemic: L. bompu (Sondhi Ohler 2011), L. boringii (Liu 1945), L. guangxiense (Fei, Mo, Ye Jiang 2009), L. hainanense (Ye Fei 1993), L. huashen (Fei Ye 2005), L. leishanense (Liu Hu 1973), L. liui (Pope 1947), L. promustache (Rao, Wilkinson Zhang 2006) and L. tengchongense (Yang, Wang Chan 2016). These species have different morphologies, narrow distribution areas, and their taxonomy is subject to controversy (AmphibiaChina 2019). The megophryid genus Leptobrachium was considered to contain two subgenera Vibrissaphora and Leptobrachium (Matsui et al. 2010). Five Leptobrachium species, L. ailaonicum, L. boringii, L. leishanense, L. liui, and L. promustache, were originally classified as Vibrissaphora, based on adult males bearing spines on the upper lip (Fei Ye 2016). However, recent phylogenetic studies showed that Vibrissaphora was not a subgenus and placed within the genus Leptobrachium (Zheng et al. 2008; Matsui et al. 2010).
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http://dx.doi.org/10.11646/zootaxa.4732.4.8DOI Listing
February 2020

[Multi-modality MRI parameters correlate with the expression of the P504s protein in prostate cancer].

Zhonghua Nan Ke Xue 2019 Jul;25(7):619-625

Department of Radiology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China.

Objective: To evaluate the expressions of P504s, 34β-E12, and P63 proteins in PCa and the correlation between the parameters of multi-modality MRI and the expression of P504s.

Methods: We retrospectively analyzed the multi-modality MRI data on 43 PCa and 64 non-PCa patients. We obtained the signal intensity-time (SI-T) curves, maximum SI (SImax), time to SImax (Tmax), rate of maximum enhancement (Rmax), and automatically generated apparent diffusion coefficient (ADC) values by conventional, diffusion-weighted and dynamic contrast-enhanced MRI, and determined the expressions of P504s, 34β-E12 and P63 in the prostatic tissues of the patients by immunohistochemistry.

Results: Statistically significant differences were observed between the PCa and non-PCa groups in the positive expressions of P504s (83.7% vs 0%, P < 0.05), 34β-E12 (25.6% vs 91.0%, P < 0.05) and P63 (25.6% vs 86.0%, P < 0.05) in the prostatic tissue, the ADC value ([0.83 ± 0.22] vs [1.34 ± 0.28] ×10-3mm2/s, P < 0.05), Tmax ([21.30 ± 10.78] vs [50.22 ± 36.31] s, P < 0.05), SImax ([1.75 ± 0.39]% vs [1.24 ± 0.41]%, P < 0.05), and Rmax ([20.20 ± 15.50]% vs [7.98 ± 6.25]%, P < 0.05). The expression of P504s was correlated negatively with the ADC value and Tmax (r = -0.60 and -0.37, P < 0.01) but positively with SImax and Rmax (r = 0.50 and 0.45, P < 0.01).

Conclusions:  The parameters of multi-modality MRI are correlated with the expression of P504s in PCa and can be used as imaging biomarkers for predicting the degrees of its malignancy.
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July 2019

The combined effects of temperature and aromatase inhibitor on metamorphosis, growth, locomotion, and sex ratio of tiger frog () tadpoles.

PeerJ 2020 20;8:e8834. Epub 2020 Mar 20.

College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, P.R. China.

Background: The tiger frog () is widely raised by many farms in southern region of China as an economically edible frog. The growth, development, and sexual differentiation of amphibians are influenced by temperature and steroid hormone level. However, the problem of hormone residues is caused by the addition of exogenous hormones in frog breeding, it is worth considering whether non-sterol aromatase inhibitors can be used instead of hormones.

Methods: In our study, tadpoles were subjected to two water temperatures (29 °C and 34 °C) and three letrozole concentrations in the feed (0, 0.1 and 1 mg/g) to examine the effects of temperature, aromatase inhibitor and their interaction on metamorphosis, locomotion, and sex ratios. A -test and contingency table were used to analyze the metamorphosis rate of tadpoles and the survival rate of froglets after feeding for 90 days. A -test was also used to analyze sex ratios in different treatment groups.

Results: Metamorphosis time and body size (snout-vent length, body mass and condition factor) were significantly different between the two temperature treatments. Metamorphosis time was longer and body size was increased at 29 °C compared to those at 34 °C. Letrozole concentration and the temperature × letrozole interaction did not affect these variables. The jumping distance of froglets following metamorphosis was positively associated with the condition factor; when controlling for condition factor, jumping distance was not affected by temperature, letrozole concentration and their interaction. Temperature and letrozole concentration also did not affect metamorphosis and survival rate. Sex ratio of the control group (0 mg/g letrozole) was 1:1 at 29 °C, but there were more males at 34 °C. The sex ratios of treated with letrozole at 29 °C and 34 °C were significantly biased toward males, and male ratio increased as letrozole concentration increased. Furthermore, more males were produced at 34 °C than at 29 °C at each letrozole concentration.
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http://dx.doi.org/10.7717/peerj.8834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087491PMC
March 2020

[Small-cell carcinoma of the prostate: A report of 2 cases and review of the literature].

Zhonghua Nan Ke Xue 2019 Feb;25(2):150-153

Department of Urology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510120, China.

Objective: To investigate the clinicopathological characteristics and improve the clinical treatment of prostatic small-cell carcinoma (PSCC).

Methods: We reported 2 cases of PSCC derived from prostate cancer after treated by androgen blockade and prostate electrotomy and reviewed the relevant literature.

Results: Two patients with PSA elevation were diagnosed with prostate cancer by prostatic puncture biopsy and treated by maximum androgen blockade, which reduced their total PSA to the normal level. Later, due to difficult urination, they both underwent prostate electrotomy, followed by chemotherapy or radiotherapy for PSCC confirmed by postoperative pathology. Nevertheless, they died at 8 to 9 months after the discovery of PSCC.

Conclusions: PSCC can derive from prostate cancer after treatment, which may be attributed to the pathological mutation induced by long-term endocrine therapy. PSCC is more malignant than prostate cancer, and its prognosis is poor.
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February 2019

Acoustic divergence in advertisement calls among three sympatric species from East China.

PeerJ 2020 11;8:e8708. Epub 2020 Mar 11.

Laboratory of Amphibian Diversity Investigation, College of Ecology, Lishui University, Lishui, Zhejiang, P.R. China.

Background: Species-specific advertisement calls are the main mechanism of transmitting information between individuals in anuran amphibians and are therefore indispensable for anuran survival and reproduction. Survey methods that monitor these calls can be used for rapid species recognition, behavioral experiments, and conservation monitoring. In this study, we described in detail 10 call parameters from three sympatric species in the genus and analyzed the differences in call parameter among these species to provide a basis for systematic monitoring, acoustic analysis and taxonomic study of this genus.

Methods: The quantitative analyses of temporal and spectral call parameters were used in our study for the advertisement calls of three sympatric species (, and ) in Zhejiang Province, East China.

Results: Our results showed the following: (1) Significant differences existed among the three sympatric species in call duration (CD), call interval (CI), number of pulses (NP), pulse rate, call intensity (CIT), dominant frequency (DF) and frequency of the first to fourth formants (F1, F2, F3 and F4). (2) Some spectral parameters (DF, F1 and F3) were negatively correlated with the body size of the vocalizing individuals in each species. (3) The coefficients of variation within individuals (CV) for CIT, DF and F1-F4 were smaller than 5%, whereas the CV for CI was larger than 10% in each species. (4) The principal component analysis and discriminant function analysis showed that call parameters could distinguish the three species. (5) The phylogenetic generalized least squares analysis showed that phylogenetic relationships affected CD and NP against snout-vent length (SVL), DF and NP against CD, and NP against DF, but not of DF against SVL; based on the phylogenetic analysis, CD and NP were not related to SVL, but DF was negatively related to SVL.
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http://dx.doi.org/10.7717/peerj.8708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071819PMC
March 2020

The association of coagulation indicators with in-hospital mortality and 1-year mortality of patients with sepsis at ICU admissions: A retrospective cohort study.

Clin Chim Acta 2020 May 7;504:109-118. Epub 2020 Feb 7.

Department of Intensive Care Unit, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address:

Background: Coagulation activation is the host's response to pathogens during sepsis and is considered to be one of the reasons for tissue damage and multiple organ failure. This study is designed to evaluate whether the alterations of coagulation indicators are related to in-hospital mortality and 1-year mortality of patients with sepsis.

Method: Data of all 2258 patients were extracted from the database Multiparameter Intelligent Monitoring in Intensive Care III (MIMIC III). The relationship between the in-hospital mortality of patients with sepsis and coagulation indicators was analyzed with a receiver operating characteristic (ROC) curve analysis and logistic regression model. Effects of coagulation indicators on patients' 1-year mortality were determined by using a Cox hazard regression model, and clinical experience or quintiles were used to classify the activated partial thromboplastin time (APTT) to determine the cutoff values to explore segmentation effects.

Result: International normalized ratio (INR) was positively associated with hospital mortality of patients with sepsis after adjusting confounders with an odds ratio (OR) of 1.86 [95% confidence interval (CI), 1.37-2.52], and a hazard ratio (HR) of 1.465[95%CI(1.24-1.74)] for 1-year mortality, respectively. 1-year mortality of patients with sepsis demonstrated a U-shaped relationship with APTT, ranging from 25 to 37, indicating the lowest risk. The adjusted HR (95% CI) values for 1-year mortality of septic patients with risk values <25 and >37 were 1.493 (1.02, 2.19) and 1.379 (1.06, 1.79), respectively.

Conclusion: Increased INR in critically ill septic patients is related to greater in-hospital mortality and 1-year mortality. A U-shaped relationship was found between APTT and 1-year mortality of patients with sepsis.
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http://dx.doi.org/10.1016/j.cca.2020.02.007DOI Listing
May 2020

A coaxial film capacitor with a novel structure to enhance its flashover performance.

Rev Sci Instrum 2019 Dec;90(12):124709

State Key Laboratory of Intense Pulsed Radiation Simulation and Effect, Northwest Institute of Nuclear Technology, P.O. Box 69 Branch 20, Xi'an 710024, China.

As one of the key components of an electromagnetic pulse simulator, the peaking capacitor is coaxially constructed with a laminate structure utilizing alternative thin metal rings and polymer film dielectrics, which is designed as a part of the antenna. This paper presents a coaxial film capacitor with a novel structure to improve its flashover performance. First, the relationship between the capacitance and the capacitor's structural parameters are deduced, and a theoretical basis of lengthening the polymer film dielectrics to enhance the flashover performance is discussed. Based on the theoretical analysis, two 150 pF, 3-layer coaxial film capacitors with different extended lengths for the polymer film dielectrics (10 and 60 mm) are designed and developed. For the capacitor with a 60 mm extended film dielectric, the polymethylmethacrylate rings are adhered to the thin metal rings to support the polymer film dielectrics using a soft adhesive. Electric field analyses show that a longer extended length could decrease the surface field at the edge of the polymer film dielectric by as much as 93%. Comparative insulation experiments show that the flashover voltage for the improved capacitor is 89.9% higher than the original one. The influence of the capacitor's polymer extensions (including the polymer supporting rings and the film dielectrics) on the radiating field of a cone antenna is analyzed numerically, and the results show that only slight changes are introduced into the radiating field. Finally, a designed 350 pF, 20-layer coaxial film capacitor with lengthened film dielectrics is presented.
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http://dx.doi.org/10.1063/1.5118790DOI Listing
December 2019

Bleomycin induces epithelial-to-mesenchymal transition via bFGF/PI3K/ESRP1 signaling in pulmonary fibrosis.

Biosci Rep 2020 01;40(1)

Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, People's Republic of China.

Idiopathic pulmonary fibrosis (IPF) is a fatal and chronic disease with a high rate of infection and mortality; however, its etiology and pathogenesis remain unclear. Studies have revealed that epithelial-mesenchymal transition (EMT) is a crucial cellular event in IPF. Here, we identified that the pulmonary fibrosis inducer bleomycin simultaneously increased the expression of bFGF and TGF-β1 and inhibited epithelial-specific regulatory protein (ESRP1) expression in vivo and in vitro. In addition, in vitro experiments showed that bFGF and TGF-β1 down-regulated the expression of ESRP1 and that silencing ESRP1 promoted EMT in A549 cells. Notably, we determined that bFGF activates PI3K/Akt signaling, and treatment with the PI3K/Akt inhibitor LY294002 inhibited bleomycin-induced cell morphology changes and EMT. In addition, the effects of LY294002 on bleomycin-induced EMT were inhibited by ESRP1 silencing in A549 cells. Taken together, these findings suggest that bleomycin induced EMT through down-regulating ESRP1 by simultaneously increasing bFGF and TGF-β1 in pulmonary fibrosis. Additionally, our findings indicated that bFGF inhibits ESRP1 by activating PI3K/Akt signaling.
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http://dx.doi.org/10.1042/BSR20190756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960066PMC
January 2020

TMPRSS4 Drives Angiogenesis in Hepatocellular Carcinoma by Promoting HB-EGF Expression and Proteolytic Cleavage.

Hepatology 2020 09 30;72(3):923-939. Epub 2020 Jun 30.

Department of General Surgery, Qilu Hospital, Shandong University, Jinan, China.

Background And Aims: Heparin-binding epidermal growth factor (HB-EGF), a member of the epidermal growth factor family, plays a pivotal role in the progression of several malignancies, but its role and regulatory mechanisms in hepatocellular carcinoma (HCC) remain obscure. Here, we report that transmembrane protease serine 4 (TMPRSS4) significantly enhanced the expression and proteolytic cleavage of HB-EGF to promote angiogenesis and HCC progression.

Approach And Results: A mechanistic analysis revealed that TMPRSS4 not only increased the transcriptional and translational levels of HB-EGF precursor, but also promoted its proteolytic cleavage by enhancing matrix metallopeptidase 9 expression through the EGF receptor/Akt/mammalian target of rapamycin/ hypoxia-inducible factor 1 α signaling pathway. In addition, HB-EGF promoted HCC proliferation and invasion by the EGF receptor/phosphoinositide 3-kinase/Akt signaling pathway. The level of HB-EGF in clinical samples of serum or HCC tissues from patients with HCC was positively correlated with the expression of TMPRSS4 and the microvessel density, and was identified as a prognostic factor for overall survival and recurrence-free survival, which suggests that HB-EGF can serve as a potential therapeutic target for HCC. More importantly, we provide a demonstration that treatment with the HB-EGF inhibitor cross-reacting material 197 alone or in combination with sorafenib can significantly suppress angiogenesis and HCC progression.

Conclusions: HB-EGF can be regulated by TMPRSS4 to promote HCC proliferation, invasion, and angiogenesis, and the combination of the HB-EGF inhibitor cross-reacting material 197 with sorafenib might be used for individualized treatment of HCC.
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http://dx.doi.org/10.1002/hep.31076DOI Listing
September 2020

Differentiating Nonfunctional Paraganglioma of the Bladder from Urothelial Carcinoma of the Bladder: Pitfalls and Breakthroughs.

Biomed Res Int 2019 6;2019:1097149. Epub 2019 Nov 6.

Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Background: Although both nonfunctional paraganglioma of the bladder (NPB) and urothelial carcinoma of the bladder (UCB) are subtypes of bladder tumors, they are entirely different entities with distinct tissue origins and anatomical locations. However, NPB is frequently misdiagnosed as UCB chiefly due to the similarities in their clinical characteristics and cystoscopic features. This study aimed to compare the differences in their clinical characteristics and cystoscopic features.

Patients And Methods: Between April 2007 and September 2017, 14 patients with NPB (NPB group) were retrieved from 2 centers, and 42 patients with new-onset UCB (UCB group) were randomly retrieved. Demographic, symptomatic, imaging, and cystoscopic data of patients in both groups were collected and compared.

Results: NPB group comprised 7 males and 7 females, with a mean age of 43.1 ± 13.6 years. Compared with the UCB group, patients in the NPB group were significantly younger ( < 0.001), less likely to be male ( < 0.05), and to present with hematuria ( < 0.01). However, no significant difference in maximum tumor diameter was observed between the 2 groups (=0.609). Compared with the UCB group, cystoscopically, patients in the NPB group were significantly more likely to present with hypervascularization but less likely to present with hemorrhage, necrosis, calcification, pedunculation, and multilesion ( < 0.05). No patients with NPB were clinically diagnosed correctly before cystoscopy. Of the 5 patients who underwent both cystoscopy and biopsy, 4 were diagnosed with NPB, while 1 remained undiagnosed. Of the remaining 9 patients who underwent cystoscopy only, 5 were diagnosed with nonepithelial tumor, and 4 were misdiagnosed with UCB.

Conclusions: Age, sex, and hematuria may provide clues to differentiating NPB from UCB. Differences in cystoscopic features between NPB and UCB are of high diagnostic value. Cystoscopic biopsy should be considered in the preoperative diagnosis of NPB.
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http://dx.doi.org/10.1155/2019/1097149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874976PMC
April 2020

Chinese herbal medicine for epidermal growth factor receptor inhibitor-induced skin rash in patients with malignancy: An updated meta-analysis of 23 randomized controlled trials.

Complement Ther Med 2019 Dec 9;47:102167. Epub 2019 Sep 9.

The First Clinical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, China; The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China. Electronic address:

Objective: To systematically review and evaluate the effectiveness of Chinese herbal medicine (CHM) therapy for epidermal growth factor receptor inhibitor (EGFRI)-induced skin rash in patients with malignancy.

Methods: The electronic databases of Medline, PubMed, EMBASE, the Cochrane Library, China National Knowledge Infrastructure, VIP Chinese Sci-tech Journal, Wan Fang, and Chinese Biomedicine were searched from their inception to 31 st September 2018. Randomized controlled trials (RCTs) investigating the effectiveness of CHM in improving EGFRI-induced skin rash were analyzed by Review Manager 5.3.

Results: Twenty-three eligible RCTs with 1392 participants were identified and divided into four subgroups according to different treatment rules of Traditional Chinese Medicine (TCM) and different controls. CHM (dispel wind, clear heat, and eliminate dampness), the representative formula Xiao Feng San, is more effective than western medicine in improving and curing skin rash(RR,95%CI: 1.46,1.26-1.70 and 1.65,1.24-2.20); CHM (nourish yin, clear heat, and remove toxin for eliminating blood stasis), the representative formula Yang Fei Xiao Zhen Tang, is more effective than western medicine in improving skin rash(RR,95%CI: 1.45,1.10-1.92). CHM (clear lung and purge heat, cool blood, and remove toxic substance) is more effective in improving and curing skin rash, compared with the western medicine group (RR,95%CI: 1.42,1.21-1.67 and 2.43,1.23-4.81) or the blank control group(RR,95%CI:2.37,1.21-4.63 and 2.98,1.20-7.41). The side effects of CHM are all mild and tolerable. Sensitivity analysis indicates that the results of the study are stable. The asymmetry funnel plots described that publication bias of this research may exist.

Conclusion: The limited evidence suggests that CHM exhibits clinical effectiveness and good safety on the treatment of EGFRI-induced skin rash. Large-sample RCTs are required to further determine the effectiveness of CHM.
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http://dx.doi.org/10.1016/j.ctim.2019.08.001DOI Listing
December 2019

The advertisement call of Leptobrachella liui Fei and Ye, 1990 (Anura: Megophryidae).

Zootaxa 2019 Apr 3;4576(3):zootaxa.4576.3.11. Epub 2019 Apr 3.

Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, Jiangsu, China ADI Team, College of Ecology, Lishui University, Lishui 323000, Zhejiang, China.

Leptobrachella (Anura: Megophryidae) is currently composed of seventy-one species distributed in China and Southeast Asia (Frost 2018). Fourteen species of Leptobrachella are currently known from southern China: L. alpina (Fei, Ye Li, 1990), L. laui (Sung, Yang Wang, 2014), L. liui (Fei Ye, 1990), L. mangshanensis (Hou, Zhang, Hu, Li, Shi, Chen, Mo Wang, 2018), L. maoershanensis (Yuan, Sun, Chen, Rowley Che, 2017), L. oshanensis (Liu, 1950), L. purpura (Yang, Zeng Wang, 2018), L. tengchongensis (Yang, Wang, Chen Rao, 2016), L. wuhuangmontis (Wang, Yang Wang, 2018), L. yingjiangensis (Yang, Zeng Wang, 2018), L. yunkaiensis (Wang, Li, Lyu Wang, 2018), L. ventripunctata (Fei, Ye Li, 1990), L. pelodytoides (Boulenger, 1893) and L. sungi (Lathrop, Murphy, Orlov Ho, 1998) and the first eleven species are endemic of this region (AmphibiaChina 2018). Many new species in this genus were discovered based on molecular, morphometric and bioacoustic data in recent years (Frost 2018). In comparison to molecular and morphological data, bioacoustics is especially useful in species identification of Leptobrachella in field surveys (Rowley et al. 2016). Although the advertisement calls of many Leptobrachella species were described along with the species descriptions (e.g., Rowley et al. 2016), the advertisement calls of only three Chinese endemic species (L. alpinus; L. laui and L. oshanensis) are known (Jiang et al. 2002; Matsui 2006; Xu et al. 2005). Here, we describe the advertisement calls of L. liui, which inhabits rocky streams in southeastern China (Fujian, Jiangxi and Zhejinag) and is morphologically diagnosed by the combination of a set of external color traits (Fig. 1).
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http://dx.doi.org/10.11646/zootaxa.4576.3.11DOI Listing
April 2019

[Clinical Value of Peripheral Blood Circulating Tumor Cells Detection in Early Cervical Infiltrating Carcinoma Screening].

Sichuan Da Xue Xue Bao Yi Xue Ban 2019 May;50(3):415-419

Department of Pathology, Zhongshan Boai Hospital Affiliated to Southern Medical University, Zhongshan 528400, China.

Objective: To observe the difference of circulating tumor cells (CTC) in peripheral blood of patients with different degrees of cervical lesions, and to evaluate the effectiveness of CTC detection in screening early invasive cervical cancer.

Methods: From December 2015 to October 2017, 63 cases of cervicitis, low-grade and high-grade intraepithelial lesions (LSIL, HSIL) and early invasive cervical cancer were confirmed by histopathological and clinical stages in Zhongshan Boai Hospital and Zhongshan Hospital Affiliated to Zhongshan University. The immunomagnetic bead negative enrichment technique combined with immunofluorescence was used. hybridization (imFISH) was used to detect CTC in peripheral blood of patients. The positive rate and quantity of CTC in four groups were analyzed. The diagnostic efficacy of CTC in early invasive cervical cancer was evaluated based on the results of histopathological diagnosis and clinical staging.

Results: ①The positive rates of CTC in cervicitis group, LSIL group, HSIL group and early invasive cervical cancer group were 0, 0, 19.05% and 84.13% respectively. The overall difference was statistically significant ( =504.00, <0.05). The positive rate of CTC in early invasive cervical cancer group was higher than that in other groups ( <0.008 3). The positive rates of CTC in HSIL group were significantly different from those in LSIL group and cervicitis group ( <0.008 3). ②The average number (median) of CTC positive in cervicitis group, LSIL group, HSIL group and early invasive cervical cancer group was 0, 0, 1/4 mL, 3/4 mL, respectively. The average number of positive CTC in early invasive cervical cancer group was higher than that in other groups, and the difference was significant compared with other groups ( <0.008 3). The average number of CTC positive in HSIL group was significantly different from that in LSIL and cervicitis group ( <0.008 3). ③The sensitivity, specificity, coincidence rate, positive predictive value and negative predictive value of CTC positive results in the diagnosis of early invasive cervical cancer were 84.13%, 93.65%, 91.27%, 81.54% and 94.65%, respectively.

Conclusion: CTC exists in patients with HSIL and early invasive cervical cancer. With the aggravation of cervical lesions, the positive rate and number of CTC test results increase. CTC detection in early invasive cervical cancer screening has a certain practical value in clinic.
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May 2019

Increased Prediction Ability in Norway Spruce Trials Using a Marker X Environment Interaction and Non-Additive Genomic Selection Model.

J Hered 2019 12;110(7):830-843

Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.

A genomic selection study of growth and wood quality traits is reported based on control-pollinated Norway spruce families established in 2 Northern Swedish trials at 2 locations using exome capture as a genotyping platform. Nonadditive effects including dominance and first-order epistatic interactions (including additive-by-additive, dominance-by-dominance, and additive-by-dominance) and marker-by-environment interaction (M×E) effects were dissected in genomic and phenotypic selection models. Genomic selection models partitioned additive and nonadditive genetic variances more precisely than pedigree-based models. In addition, predictive ability in GS was substantially increased by including dominance and slightly increased by including M×E effects when these effects are significant. For velocity, response to genomic selection per year increased up to 78.9/80.8%, 86.9/82.9%, and 91.3/88.2% compared with response to phenotypic selection per year when genomic selection was based on 1) main marker effects (M), 2) M + M×E effects (A), and 3) A + dominance effects (AD) for sites 1 and 2, respectively. This indicates that including M×E and dominance effects not only improves genetic parameter estimates but also when they are significant may improve the genetic gain. For tree height, Pilodyn, and modulus of elasticity (MOE), response to genomic selection per year improved up to 68.9%, 91.3%, and 92.6% compared with response to phenotypic selection per year, respectively.Subject Area: Quantitative genetics and Mendelian inheritance.
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http://dx.doi.org/10.1093/jhered/esz061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6916663PMC
December 2019

Evaluation of the sensitivity of Microhyla fissipes tadpoles to aqueous cadmium.

Ecotoxicology 2019 Dec 16;28(10):1150-1159. Epub 2019 Oct 16.

ADI, College of Ecology, Lishui University, 323000, Lishui, Zhejiang, People's Republic of China.

Cadmium (Cd) exposure is harmful to amphibians in natural environments and the Cd concentration is a key parameter in water monitoring. Cd pollution has been a severe issue in the Yangtze River and its southern reaches in recent years. Acute toxicity assays were employed to determine the tolerance limits of Cd for Microhyla fissipes tadpoles and five different concentrations of Cd (0, 50, 100, 200 and 300 μg/L) were involved to detect its chronic effects on metamorphosis, growth, locomotion, genotoxicity and enzymatic activities of M. fissipes tadpoles. The results showed that the 24-h and 48-h LC values of Cd on M. fissipes tadpoles were 2591.3 μg/L and 1567.9 μg/L, respectively, and the presumable non-lethal concentration obtained was 172.2 μg/L. During the 70-day chronic toxicity assays, Cd showed negative impacts on survival, growth, metamorphosis and the frequency of erythrocytes nuclear abnormality of M. fissipes tadpoles. However, the Cd exposure caused the increased body size and condition of tadpoles at complete metamorphosis (GS46). The tadpoles exposed to 200 μg/L of Cd exhibited degraded locomotor performance at GS46. Weight increments of tadpoles were inhibited at Day 14 and massive deaths were observed over the next 14 days. The enzymatic activities of tadpoles experienced a shock response stage (GS30-GS35) and a complete recovery stage (GS36-GS41) in all treatments. However, the enzymatic activities (except alkaline phosphatase) of tadpoles at GS46 increased after Cd exposure, especially at high concentrations. In summary, Cd is a threat to M. fissipes tadpoles as that causes reduced fitness.
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http://dx.doi.org/10.1007/s10646-019-02117-yDOI Listing
December 2019

Genome-wide association study identified novel candidate loci affecting wood formation in Norway spruce.

Plant J 2019 10 28;100(1):83-100. Epub 2019 Jul 28.

Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Science, Parallellvägen 21, Umeå, 907 36, Sweden.

Norway spruce is a boreal forest tree species of significant ecological and economic importance. Hence there is a strong imperative to dissect the genetics underlying important wood quality traits in the species. We performed a functional genome-wide association study (GWAS) of 17 wood traits in Norway spruce using 178 101 single nucleotide polymorphisms (SNPs) generated from exome genotyping of 517 mother trees. The wood traits were defined using functional modelling of wood properties across annual growth rings. We applied a Least Absolute Shrinkage and Selection Operator (LASSO-based) association mapping method using a functional multilocus mapping approach that utilizes latent traits, with a stability selection probability method as the hypothesis testing approach to determine a significant quantitative trait locus. The analysis provided 52 significant SNPs from 39 candidate genes, including genes previously implicated in wood formation and tree growth in spruce and other species. Our study represents a multilocus GWAS for complex wood traits in Norway spruce. The results advance our understanding of the genetics influencing wood traits and identifies candidate genes for future functional studies.
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http://dx.doi.org/10.1111/tpj.14429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852177PMC
October 2019
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