Publications by authors named "Zhi-Peng Li"

64 Publications

Histone methylatic modification mediates the tumor-suppressive activity of curcumol in hepatocellular carcinoma via an Hotair/EZH2 regulatory axis.

J Ethnopharmacol 2021 Nov 12;280:114413. Epub 2021 Jul 12.

Key Laboratory of Orthopaedics and Traumatology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, China; Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou, 510405, PR China. Electronic address:

Ethnopharmacological Relevance: Curcuma kwangsiensis S. G. Lee & C. F. Liang (Guangxi ezhu, in Chinese) has been used as a traditional Chinese medicine (TCM) for approximately 2000 years. Curcumol is one of the major bioactive components of this herb, which has been demonstrated possesses anti-cancer properties, and was recorded in the Chinese Pharmacopoeia 2020 edition. However, most studies mainly focused on the superficial anti-cancer activity, the underlying mechanism remains poorly understood.

Aim Of The Study: In the present study, we aimed to investigate the anti-tumor effect of Curcumol on hepatocellular carcinoma (HCC), and elucidate its underlying mechanism from the perspective of epigenetic modification.

Materials And Methods: The potential anti-cancer properties of Curcumol were evaluated in HepG2 and SMMC-7721 cells. Its effects on cell growth, cell cycle, apoptosis and migration were examined in these HCC cells. Moreover, the lncRNA HOX transcript antisense intergenic RNA (Hotair) and histone methylatic modification were detected by qPCR and Western blotting assays.

Results: In the present study, Curcumol was illustrated to suppress cell growth in HCC cells via inducing apoptosis and cell cycle arrest. And it was also found that Curcumol inhibited the invasion and metastasis of HCC as well. As for the mechanism investigation, it was showed that lncRNA Hotair was significantly downregulated by Curcumol in HCC cells. As is well known, Hotair recruited histone methyltransferase enhancer of zeste homolog 2 (EZH2) to exert transcriptional regulation. Our results showed that EZH2 were downregulated by Curcumol in HCC cells, and thus disrupted the trimethylation of H3K9 and H3K27 which were specifically catalyzed by EZH2.

Conclusions: In conclude, our results demonstrated that Curcumol suppressed tumor growth and metastasis via an Hotair/EZH2/histone modification regulatory axis.
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http://dx.doi.org/10.1016/j.jep.2021.114413DOI Listing
November 2021

Access to quality diagnosis and rational treatment for tuberculosis: real-world evidence from China-Gates Tuberculosis Control Project Phase III.

Infect Dis Poverty 2021 Jun 29;10(1):92. Epub 2021 Jun 29.

Duke Global Health Institute, Duke University, 310 Trent Drive, Durham, NC, 27710, USA.

Background: China has successfully reduced tuberculosis (TB) incidence rate over the past three decades, however, challenges remain in improving the quality of TB diagnosis and treatment. In this paper, we assess the effects of the implementation of "China National Health Commission (NHC) and Gates Foundation TB Prevention and Control Project" on the quality of TB care in the three provinces.

Methods: We conducted the baseline study in 2016 and the final evaluations in 2019 in the 12 selected project counties. We obtained TB patients' information from the TB Information Management System and reviewed medical records of TB cases in the TB designated hospitals. We compared TB diagnosis and treatment services with the national practice guideline and used Student's t-test and Pearson χ tests or Fisher's exact tests to compare the difference before and after the project implementation.

Results: The percentage of sputum smear-negative (SS-) patients taking culture or rapid molecular test (RMT) doubled between 2015 and 2018 (from 35% to 87%), and the percentage of bacteriologically confirmed pulmonary TB cases increased from 36% to 52%. RMT has been widely used and contributed an additional 20% of bacteriologically confirmed TB cases in 2018. The percentage of TB patients taking drug susceptibility tests (DST) also doubled (from 40% in 2015 to 82% in 2018), and the proportion of TB patients receiving adequate diagnosis services increased from 85% to 96%. Among all SS- TB patients, over 86% received the recommended diagnostic services at the end of the study period, an improvement from 75% prior to the project implementation. However, the proportion of TB patients treated irrationally using second-line anti-TB drugs (SLDs) increased from 12.6% in 2015 to 19.9% in 2018. The regional disparities remained within the project provinces, albeit the gaps between them narrowed down for almost all indicators.

Conclusions: The quality of TB diagnosis services has been improved substantially, which is attributable to the coverage of new diagnosis technology. However, irrational use of SLDs remains a concern after the project implementation.
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http://dx.doi.org/10.1186/s40249-021-00875-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243738PMC
June 2021

Impacts of a comprehensive tuberculosis control model on the quality of clinical services and the financial burden of treatment for patients with drug-resistant tuberculosis in China: a mixed-methods evaluation.

Infect Dis Poverty 2021 Apr 21;10(1):54. Epub 2021 Apr 21.

Duke Global Health Institute, Duke University, 310 Trent Drive, Durham, NC, 27710, USA.

Background: The China National Health Commission-Gates TB Project Phase III implemented a comprehensive TB control model including multiple interventions to address the burden of drug-resistant TB (DRTB). This study aims to evaluate the quality of DRTB clinical services and assess the financial burden of DRTB patients during the intervention period.

Methods: A mixed-methods approach was used to evaluate the effectiveness of interventions in the three project provinces: Zhejiang, Jilin and Ningxia Hui Autonomous Region. The quantitative data included de-identified DRTB registry data during 2015-2018 in project provinces from China CDC, medical records of DRTB patients registered in 2018 (n = 106) from designated hospitals, and a structured DRTB patient survey in six sample prefectures in 2019. The quality of clinical services was evaluated using seven indicators across patient screening, diagnosis and treatment. Logistic regression was conducted to explore factors associated with the extremely high financial burden. Semi-structured in-depth interviews with policymakers and focus group discussions with physicians and DRTB patients were conducted to understand the interventions implemented and their impacts.

Results: The percentage of bacterially confirmed patients taking a drug susceptibility test (DST) increased significantly between 2015 and 2018: from 57.4 to 93.6% in Zhejiang, 12.5 to 86.5% in Jilin, and 29.7 to 91.4% in Ningxia. The treatment enrollment rate among diagnosed DRTB patients also increased significantly and varied from 73 to 82% in the three provinces in 2018. Over 90% of patients in Zhejiang and Jilin and 75% in Ningxia remained in treatment by the end of the first six months' treatment. Among all survey respondents 77.5% incurred extremely high financial burden of treatment. Qualitative results showed that interventions on promoting rapid DST technologies and patient referral were successfully implemented, but the new financing policies for reducing patients' financial burden were not implemented as planned.

Conclusions: The quality of DRTB related clinical services has been significantly improved following the comprehensive interventions, while the financial burden of DRTB patients remains high due to the delay in implementing financing policies. Stronger political commitment and leadership are required for multi-channel financing to provide additional financial support to DRTB patients.
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http://dx.doi.org/10.1186/s40249-021-00832-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059277PMC
April 2021

[Methodological Research on Rapid Detection and Genotyping of NPM1 Gene Mutations in Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Apr;29(2):403-407

College of Engineering, Beijing Normal University, Zhuhai 519087, Guangdong Province, China,E-mail:

Objective: To establish a method for rapid detection and typing of NPM1 mutations in acute myeloid leukemia (AML) by fluorescence melting curve analysis technology.

Methods: A pair of primers and a fluorescent single-stranded probe (molecule beacon) were designed for the mutant genes mutA, mutB, mutD in exon 12 of nucleopsin (NPM1) and wild type. With a real-time qPCR, the A, B, and D gene mutations of NPM1 were detected and typed by different-melting curve peak value of the probe through RT-PCR.

Results: This method could detected the mutations of A, B, and D in NPM1 effectively with a sensitivity of 1%. Furthermore, 62 AML clinical samples were evaluated by the method. In the results, the detection rate and typing of NPM1 mutations were consistent with the sequencing results of clinical samples.

Conclusion: There are three features in the method of fluorescence melting curve analysis: stable PCR system, easy to operate, and the easily distinguishable results. The method might meet the demand for rapid typing of NPM1 gene mutation in early diagnosis or concomitant diagnosis of AML.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.02.015DOI Listing
April 2021

HDAC6 Is Involved in the Histone Deacetylation of In Vitro Maturation Oocytes and the Reprogramming of Nuclear Transplantation in Pig.

Reprod Sci 2021 09 16;28(9):2630-2640. Epub 2021 Mar 16.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, 530005, Guangxi, China.

It remained unknown whether HDAC6 affected the histone deacetylation of in vitro maturation oocytes and the reprogramming of nuclear transplantation in pig. Our results indicated that HDAC6 specific inhibition did not affect overall HDAC activity and meiosis process, which increased histone H3K9/K14 and H4K8 acetylation of porcine in vitro maturation oocytes and pseudo-pronucleus embryos. HDAC6 inhibition also significantly enhanced the cleavage and blastocyst of nuclear transfer embryos (0.81 ± 0.12 vs. 0.68 ± 0.12 and 0.46 ± 0.19; 0.73 ± 0.13 vs. 0.63 ± 0.18 and 0.40 ± 0.16, P<0.05). The inhibition of HDAC6 significantly enhanced histone H3K9/K14 and H4K8 acetylation, and upregulated the OCT4 and CDX2 expressions (1.83 ± 0.16 vs. 1.00 ± 0.00 %; 2.07 ± 0.09 vs. 1.00 ± 0.00; P<0.05) in porcine SCNT blastocysts. Interestingly, HDAC6 inhibition significantly increased the pseudo-pronucleus volume during somatic cell reprogramming. Thus, HDAC6 was required for porcine histone deacetylation during the in vitro maturation and pseudo-pronucleus stages. HDAC6 inhibition improved the in vitro development of nuclear transfer embryos. HDAC6 may restrict the reprogramming of somatic nuclear transfer by regulating pseudo-pronucleus expansion. We need further research to confirm this in the future.
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http://dx.doi.org/10.1007/s43032-021-00533-2DOI Listing
September 2021

Efficacy of Traditional Chinese Medicine Combined with Selective Serotonin Reuptake Inhibitors on the Treatment for Parkinson's Disease with Depression: A Systematic Review and Meta-Analysis.

Am J Chin Med 2021 3;49(3):627-643. Epub 2021 Mar 3.

Department of Anatomy, School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, P. R. China.

Depression is a common neuropsychiatric symptom of Parkinson's disease (PD), resulting in a lower quality of life and cognitive impairment in PD patients. Traditional Chinese medicine (TCM) formulas have been widely used in neurodegenerative disease and neuropsychic disorders to improve life quality of patients in ethnomedicine. TCM formulas combined with selective serotonin reuptake inhibitors (SSRIs) also have a positive effect on depressed PD compared with SSRIs as reported by several clinical studies. However, the results are discordant and failed to be conclusive. We aim to evaluate the efficacy of TCM formulas combined with SSRIs for depressed PD in this systematic review. We searched literatures from PubMed, Web of Science, Medline, Embase, Google Scholar, Chinese National Knowledge Infrastructure, Wanfang Database, and VIP Information Database before July 2020. We included randomized controlled trials investigating the efficacy of TCM formulas combined with SSRIs on depressed PD patients. This analysis was according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses guideline. Eleven randomized clinical trials involving 861 subjects were enrolled in this analysis. The overall results showed that TCM formulas combined with SSRIs significantly improved the depression score [weighted mean difference (WMD): -4.920, 95% confidence interval (CI): (-5.999, -3.840); [Formula: see text]¡ 0.001] and had a statistical significance on Unified Parkinson's Disease Rating Scale II score [WMD: -1.209, 95% CI: (-1.561, -0.857); [Formula: see text] < 0.001]. Furthermore, we observed that Chai-Hu-Shu-Gan Powder combined with SSRIs had a significant improvement on the depressive symptom in PD compared to the SSRIs alone [WMD: -5.390, 95% CI: (-7.66, -3.11); [Formula: see text] < 0.001]. No severe side events were reported in these included trials. This systematic review provided the evidences that TCM formulas combined with SSRIs might be helpful and safe in the treatment of depression of PD, including Chai-Hu-Shu-Gan Powder. Also, more randomized double-blinded trials with reliable design are required in the future.
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http://dx.doi.org/10.1142/S0192415X21500282DOI Listing
September 2021

Apigenin promotes osteogenic differentiation of mesenchymal stem cells and accelerates bone fracture healing via activating Wnt/β-catenin signaling.

Am J Physiol Endocrinol Metab 2021 04 1;320(4):E760-E771. Epub 2021 Mar 1.

Key Laboratory of Orthopaedics and Traumatology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, China.

Apigenin (API), a natural plant flavone, is abundantly found in common fruits and vegetables. As a bioactive flavonoid, API exhibits several activities including antiproliferation and anti-inflammation. A recent study showed that API could retard osteoporosis progress, indicating its role in the skeletal system. However, the detailed function and mechanism remain obscure. In the present study, API was found to promote osteogenic differentiation of mesenchymal stem cells (MSCs). And further investigation showed that API could enhance the expression of the critical transcription factor β-catenin and several downstream target genes of Wnt signaling, thus activated Wnt/β-catenin signaling. Using a rat femoral fracture model, API was found to improve new bone formation and accelerate fracture healing in vivo. In conclusion, our data demonstrated that API could promote osteogenesis in vitro and facilitate the fracture healing in vivo via activating Wnt/β-catenin signaling, indicating that API may be a promising therapeutic candidate for bone fracture repair. ) API promoted osteogenic differentiation of human MSCs in vitro; ) API facilitated bone formation and accelerated fracture healing in vivo; ) API stimulated Wnt/β-catenin signaling during osteogenesis of human MSCs.
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http://dx.doi.org/10.1152/ajpendo.00543.2019DOI Listing
April 2021

Mitophagy, a Form of Selective Autophagy, Plays an Essential Role in Mitochondrial Dynamics of Parkinson's Disease.

Cell Mol Neurobiol 2021 Feb 2. Epub 2021 Feb 2.

Department of Anatomy, School of Chinese Medicine, Beijing University of Chinese Medicine, Sunshine Southern Avenue, Fang-Shan District, Beijing, 102488, China.

Parkinson's disease (PD) is a severe neurodegenerative disorder caused by the progressive loss of dopaminergic neurons in the substantia nigra and affects millions of people. Currently, mitochondrial dysfunction is considered as a central role in the pathogenesis of both sporadic and familial forms of PD. Mitophagy, a process that selectively targets damaged or redundant mitochondria to the lysosome for elimination via the autophagy devices, is crucial in preserving mitochondrial health. So far, aberrant mitophagy has been observed in the postmortem of PD patients and genetic or toxin-induced models of PD. Except for mitochondrial dysfunction, mitophagy is involved in regulating several other PD-related pathological mechanisms as well, e.g., oxidative stress and calcium imbalance. So far, the mitophagy mechanisms induced by PD-related proteins, PINK1 and Parkin, have been studied widely, and several other PD-associated genes, e.g., DJ-1, LRRK2, and alpha-synuclein, have been discovered to participate in the regulation of mitophagy as well, which further strengthens the link between mitophagy and PD. Thus, in this view, we reviewed mitophagy pathways in belief and discussed the interactions between mitophagy and several PD's pathological mechanisms and how PD-related genes modulate the mitophagy process.
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http://dx.doi.org/10.1007/s10571-021-01039-wDOI Listing
February 2021

Experimental Investigation of Quantum PT-Enhanced Sensor.

Phys Rev Lett 2020 Dec;125(24):240506

CAS Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei, 230026, China.

PT-symmetric theory is developed to extend quantum mechanics to a complex region, but it wins its great success first in classical systems, for example, optical waveguides and electric circuits, etc., because there are so many counterintuitive phenomena and striking applications, including unidirectional light transport, PT-enhanced sensors (one kind of exceptional-point-based sensor), and wireless power transfer. However, these phenomena and applications are mostly based on the ability to approach a PT-symmetric broken region, which makes it difficult to transfer them to the quantum regime, since the broken quantum PT-symmetric system has not been constructed effectively, until recently several methods have been raised. Here, we construct a quantum PT-symmetric system assisted by weak measurement, which can effectively transit from the unbroken region to the broken region. The full energy spectrum including the real and imaginary parts is directly measured using weak values. Furthermore, based on the ability of approaching a broken region, we for the first time translate the previously mentioned PT-enhanced sensor into the quantum version, and investigate its various features that are associated to the optimal conditions for sensitivity enhancement. In this experiment, we obtain an enhancement of 8.856 times over the conventional Hermitian sensor. Moreover, by separately detecting the real and imaginary parts of energy splitting, we can derive the additional information of the direction of perturbations. Our work paves the way of leading classical interesting PT phenomena and applications to their quantum counterparts. More generally, since the PT system is a subset of non-Hermitian systems, our work will be also helpful in the studies of general exception point in the quantum regime.
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http://dx.doi.org/10.1103/PhysRevLett.125.240506DOI Listing
December 2020

[Spatial heterogeneity of populations in the stony desert in the middle of southern foot of Tianshan Mountains, China.]

Ying Yong Sheng Tai Xue Bao 2020 Dec;31(12):3997-4003

Institute of Desertification Studies, Chinese Academy of Forestry, Beijing 100091, China.

Plots were set on the top, middle and bottom of the stony desert alluvial fan in the middle of southern foothills of the Tianshan Mountains. With plant height and crown size as indicators, the spatial heterogeneity of populations in the stony desert were studied using geostatistical methods. Understanding the spatial heterogeneity characteristics of populations could provide scientific basis for vegetation protection and ecological restoration in stony deserts. The results showed that had a patchy distribution at the top of the alluvial fan. At the middle and bottom of the alluvial fan, it showed a banded distribution. The band width in the middle was larger than that at the bottom. From the top to the bottom of the alluvial fan, the overall plant height and crown size of populations decreased first and then increased. The average plant height on the top, middle and bottom of the alluvial fan was 40.34, 21.07, 36.96 cm, and the crown size were 1.09, 0.80, 1.43 m, respectively. The best fitting models for plant height of were the exponential model, the exponential model, and the linear model at the top, middle and the bottom of the alluvial fan, respectively, while the best fitting models for crown size were exponential model, spherical model, and linear model. From the top to the bottom of the alluvial fan, the fractal dimension value of plant height and crown size of ranged from 1.909 to 1.889, indicating that the spatial pattern of populations was simple and the spatial homogeneity was high. From the top to the bottom of the alluvial fan, the spatial distance of the anisotropy of plant height and crown size of gradually shortened. At the top, middle and bottom of the alluvial fan, the spatial distances where the plant height and crown showed anisotropy were >60 m, 42-46 m and 23-27 m, respectively.
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http://dx.doi.org/10.13287/j.1001-9332.202012.003DOI Listing
December 2020

Experimental Observation of Coherent-Information Superadditivity in a Dephrasure Channel.

Phys Rev Lett 2020 Aug;125(6):060502

CAS Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei 230026, China.

We present an experimental approach to construct a dephrasure channel that contains both dephasing and erasure noises and can be used as an efficient tool to study the superadditivity of coherent information. Using a three-fold dephrasure channel, the superadditivity of coherent information is observed, and a substantial gap is found between the zero single-letter coherent information and zero quantum capacity. Particularly, we find that, when the coherent information of n channel uses is zero, with a larger number of channel uses the quantum capacity becomes positive. These phenomena exhibit a more obvious superadditivity of coherent information than previous works and demonstrate a higher threshold for nonzero quantum capacity. Such novel channels built in our experiment also can provide a useful platform to study the nonadditive properties of coherent information and quantum channel capacity.
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http://dx.doi.org/10.1103/PhysRevLett.125.060502DOI Listing
August 2020

Improved early development potence of in vitro fertilization embryos by treatment with tubacin increasing acetylated tubulin of matured porcine oocytes.

Mech Dev 2020 12 21;164:103631. Epub 2020 Aug 21.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, Guangxi 530005, China. Electronic address:

To improve the developmental potential of in vitro embryos is a long-term concern field for human assisted reproduction and animal in vitro embryo production practice. In the current study, we examined the effects and mechanism of an HDAC6 inhibitor, tubacin, on the maturation of porcine oocytes and in vitro development of porcine IVF embryos. It has been demonstrated the effect of tubacin on the acetylation level of α-tubulin in porcine oocytes. As a result, the maturation rate of porcine oocytes was significantly improved (P < 0.05), and the following development potent of blastocysts forming rate was also significantly increased (P < 0.05). We found that the increased acetylation of α-tubulin significantly reduced the abnormal rate of microtubule, furthermore, the proportion of mitochondria in the vicinity of in vitro fertilization (IVF) nucleus was significantly enhanced in Metaphase I (MI) and Metaphase II (MII) stages. The expression levels of microtubule assembly genes (TUBA1A, αTAT1 and MAP2) significantly up-regulated in MI and MII stages. Together, these results suggest that treatment of porcine oocytes during maturation with tubacin could promote their IVF embryos developmental competence by altering spindle formation, mitochondrial concentration and genes expression patterns of matured porcine oocytes.
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http://dx.doi.org/10.1016/j.mod.2020.103631DOI Listing
December 2020

[Performance and Membrane Fouling Characteristics of Mariculture Wastewater Treated by Anoxic MBR-MMR].

Huan Jing Ke Xue 2020 Jun;41(6):2762-2770

Ji'nan Environmental Monitoring Center, Ji'nan 250102, China.

An anoxic membrane bioreactor-microalgae membrane reactor coupling system (anoxic MBR-MMR) was used to deal with mariculture wastewater. Pre-anoxic MBR was used for the degradation of organic matter, NO-N and NO-N, and the released NH-N entered MMR for microalgae growth and was removed. Meanwhile, the treatment efficiency and the microalgae recovery were studied, and the membrane fouling behavior was investigated. After running for 91 days, the removal rates of the system toward NO-N and NH-N were stable at above 90.0% and 88.0%, respectively. Furthermore, the average removal rates of PO-P and TOC were 49.4% and 84.7%, respectively. Under the condition that the microalgae were harvested continuously, the biomass can be stably operated at an average concentration of 9×10 cells·mL and good removal efficiency and resource utilization could be achieved. Through infrared spectrum and three-dimensional fluorescence spectrum analysis, the main substances causing membrane fouling in MMR were tryptophan proteins and humic acids. The membrane fouling in MMR was lighter than that in anoxic MBR.
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http://dx.doi.org/10.13227/j.hjkx.201910057DOI Listing
June 2020

Experimental Investigation of State Distinguishability in Parity-Time Symmetric Quantum Dynamics.

Phys Rev Lett 2020 Jun;124(23):230402

CAS Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei 230026, People's Republic of China and CAS Center For Excellence in Quantum Information and Quantum Physics, University of Science and Technology of China, Hefei 230026, People's Republic of China.

Comprehensive study on parity-time (PT) symmetric systems demonstrates the novel properties and innovative application of non-Hermitian physics in recent years. In the quantum regime, PT symmetric physics exhibits unique quantum dynamical behaviors such as spontaneous state-distinguishability oscillation. However, the construction and control of a PT symmetric quantum system are still challenging, that and restrict the experimental investigation of PT symmetric quantum nature and application. In this Letter, we propose and construct a recycling-structure PT symmetric quantum simulator for the first time, which can effectively simulate the discrete-time dynamical process of a PT symmetric quantum system in both unbroken and broken phases, to be different from our previous work [J.-S. Tang, et al., Nat. Photonics 10, 642 (2016)]. We investigate the dynamical features of quantum state distinguishability based on the PT symmetric simulator. Our results demonstrate the novel PT symmetric quantum dynamics characterized by the periodical oscillation of state distinguishability in the unbroken phase, and the monotonic decay of that in the broken phase. This work also provides a practical experimental platform for the future intensive study of PT symmetric quantum dynamics.
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http://dx.doi.org/10.1103/PhysRevLett.124.230402DOI Listing
June 2020

Liver-Targeting and pH-Sensitive Sulfated Hyaluronic Acid Mixed Micelles for Hepatoma Therapy.

Int J Nanomedicine 2019 2;14:9437-9452. Epub 2019 Dec 2.

School of Bioscience and Technology, Weifang Medical University, Weifang, Shandong, People's Republic of China.

Background: The tumor-targeting ability and pH-sensitive properties of intelligent drug delivery systems are crucial for effective drug delivery and anti-tumor therapy.

Methods: In this study, sHA-DOX/HA-GA mixed micelles were designed with the following properties: sulfated hyaluronic acid (sHA) was synthesized to block cell migration by inhibiting HAase; sHA-DOX conjugates were synthesized via pH-sensitive hydrazone bond to realize DOX-sensitive release. The introduction of HA-GA conjugate could improve active-targeting ability and cellular uptake.

Results: The results showed that the mixed micelles possessed a nearly spherical shape, nanoscale particle size (217.70±0.89 nm), narrow size distribution (PDI=0.07±0.04), negative zeta potential (-31.87±0.61 mV) and pH-dependent DOX release. In addition, the sHA-DOX/HA-GA micelles exhibited concentration-dependent cytotoxicities against liver carcinoma cells (HepG2) and HeLa cells, and were shown to be effectively taken up by HepG2 cells by confocal microscopy analysis. Furthermore, the in vivo anti-tumor study showed that mixed micelles had a superior anti-tumor effect compared to that of free DOX. Further evidence obtained from the hematoxylin-eosin staining and immunohistochemistry analysis also demonstrated that sHA-DOX/HA-GA exhibited stronger tumor inhibition and lower systemic toxicity than free DOX.

Conclusion: The sHA-DOX/HA-GA mixed micelles could be a potential drug delivery system for anti-hepatoma therapy.
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http://dx.doi.org/10.2147/IJN.S214528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896933PMC
February 2020

[Spatial variation and driving factors of invasive plants in Fujian Province, China].

Ying Yong Sheng Tai Xue Bao 2019 Aug;30(8):2682-2690

Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350012, China.

Based on the field investigation data and the integration GIS spatial methods and geographical detector model, we analyzed the main and interactive effects of geographical and social environmental factors on the distribution of invasive plants in Fujian Province, China. The results showed that a total of 82 invasive plant species were recorded, with Compositae as the dominant family. Conyza canadensis, Ageratum conyzoides, and Alternanthera philoxeroides had the highest frequencies. There were more invasive species in coastal areas than in inland areas. Fuzhou and Xiamen were the hot areas for plant invaision. The invasive plants widely distributed at different altitudes, and the invasion reduced with the increasing altitude. The geographical detector analysis showed that rainfall as a natural environment factor and road density and people density as socio-economy factors were the major driving factors for the distribution of invasive plant species. The multi-factor interaction had a positive effect on the spatial distribution of invasive plants, implying the complexity of impact factors on the distribution of invasive plant species. In conclusion, the geographical detector could be used in the studies of invasive plants, and environmental factors could be also applied for monitoring the suitable establishment areas of invasive plants in Fujian Province. Our results provide a scientific basis for effective management of invasive plants.
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http://dx.doi.org/10.13287/j.1001-9332.201908.010DOI Listing
August 2019

Polysaccharide RAP Induces Macrophage Phenotype Polarization to M1 via the Notch Signaling Pathway.

Molecules 2019 May 27;24(10). Epub 2019 May 27.

School of Chinese Medicine, Hong Kong Baptist University, 7 Baptist University Road, Kowloon Tong, Hong Kong, China.

Macrophages occur in polarized phenotypes, whose characteristics determine the role they play in tumor growth. The M1 phenotype macrophages promote tumoricidal responses and suppress tumor growth. Our previous study showed that a polysaccharide isolated from Radix Astragali, named RAP, was itself non-cytotoxic but induced RAW264.7 cells' cytotoxicity against cancer cells. The current study was undertaken to determine its mechanism. Series studies was conducted to show that RAP is able to induce much higher gene expression of M1 markers, including iNOS, IL-6, TNF-a, and CXCL10, compared with the control group. When RAP-induced BMDMs were transplanted together with 4T1 tumor cells in BALB/c mice, both tumor volume and tumor weight decreased. Further studies indicated that RAP induces the Notch signaling pathway in RAW264.7 cells. The function of Notch signaling in macrophage polarization was confirmed by using γ-secretase inhibitor. These results suggested that polysaccharide RAP induces macrophage's polarization to M1 phenotype via the Notch signaling pathway.
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http://dx.doi.org/10.3390/molecules24102016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572696PMC
May 2019

[Pollutant Removal Performance and Membrane Fouling Characteristics in Marine Aquaculture Wastewater Treatment by a Microalgae Membrane Reactor].

Huan Jing Ke Xue 2019 Apr;40(4):1865-1870

PAN-CHINA Construction Group Co., Ltd., Hunan Branch, Changsha 410000, China.

In order to treat mariculture wastewater, the pollutant removal performance and membrane fouling characteristics of a microalgae membrane reactor were investigated using . After 60 days of operation, the total nitrogen (TN) and total phosphorus (TP) removal efficiency of the reactor were 73.6% and 77.9%, respectively, and the removal rates of TN and TP reached 15 g·(m·d) and 2.8 g·(m·d). The microalgae in the reactor could be enriched rapidly, with a maximum growth rate of 53.3 mg·(L·d) and a maximum biomass of 1.4 g·L. The microalgae in the reactor were harvested on day 18 and day 36; harvesting did not affect the nitrogen and phosphorus removal efficiency of the reactor. To some extent, the membrane fouling phenomenon was alleviated. The increase in the microalgae biomass would significantly increase the pollutant content of the membrane. The three-dimensional excitation-emission matrix (EEM) spectra results confirmed that tryptophan-like substances and aromatic proteins had a significant effect on membrane fouling.
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http://dx.doi.org/10.13227/j.hjkx.201808121DOI Listing
April 2019

Liver-targeted liposomes for codelivery of curcumin and combretastatin A4 phosphate: preparation, characterization, and antitumor effects.

Int J Nanomedicine 2019 8;14:1789-1804. Epub 2019 Mar 8.

School of Bioscience and Technology, Weifang Medical University, Weifang, Shandong, China,

Background: Recent efforts have been focused on combining two or more therapeutic approaches with different mechanisms to enhance antitumor therapy. Moreover, nanosize drug-delivery systems for codelivering two drugs with proapoptotic and antiangiogenic activities have exhibited great potential in efficient treatment of cancers.

Methods: Glycyrrhetinic acid (GA)-modified liposomes (GA LPs) for liver-targeted codelivery of curcumin (Cur) and combretastatin A4 phosphate (CA4P) were prepared and characterized. In vitro cellular uptake, cytotoxicity, cell migration, in vivo biodistribution, antitumor activity, and histopathological studies were performed.

Results: Compared with unmodified LPs (Cur-CA4P LPs), Cur-CA4P/GA LPs were taken up effectively by human hepatocellular carcinoma cells (BEL-7402) and showed higher cytotoxicity than free drugs. In vivo real-time near-infrared fluorescence-imaging results indicated that GA-targeted LPs increased accumulation in the tumor region. Moreover, Cur-CA4P/GA LPs showed stronger inhibition of tumor proliferation than Cur, Cur + CA4P, and Cur-CA4P LPs in vivo antitumor studies, which was also verified by H&E staining.

Conclusion: GA-modified LPs can serve as a promising nanocarrier for liver-targeted co-delivery of antitumor drugs against hepatocellular carcinoma.
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http://dx.doi.org/10.2147/IJN.S188971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413741PMC
April 2019

Polysaccharide RAP Selectively Attenuates Paclitaxel-Induced Cytotoxicity Toward RAW 264.7 Cells by Reversing Cell Cycle Arrest and Apoptosis.

Front Pharmacol 2018 11;9:1580. Epub 2019 Feb 11.

School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

The purpose of this study was to determine if an polysaccharide (RAP) can protect immune cells from the toxic side effects of paclitaxel (Taxol), a powerful anti-tumor drug whose equally powerful side effects limit its clinical use. We hypothesized that RAP can reduce the toxic effects induced by Taxol. To test this hypothesis, we conducted a series of studies and . First, we confirmed RAP's effects utilizing BALB/c mice inoculated with 4T1 mouse breast cancer cells as the tumor model. Mice were treated with RAP and/or Taxol, and the differences in the life spans were recorded. Second, a co-culture cell model was used to study the protective effect of RAP on cells vis-a-vis Taxol. The cell cycle and apoptosis of RAW 264.7 cells that were treated with RAP with/without Taxol were checked by flow cytometry and Hoechst staining. Proteins involved in the cell cycle and apoptosis were also tested by Western blot to reveal the probable mechanism. RAP prolonged the life span of tumor-bearing mice treated with Taxol. The experiments showed that Taxol suppressed the proliferation of RAW 264.7 cells while RAP protected the RAW 264.7 cells from Taxol-induced suppression. The protection is selective because RAP had no effect on 4T1 cells. Furthermore, Taxol clearly led to cell cycle arrest mainly at the G2/M phase and generated cytotoxicity against RAW 264.7 cells, while RAP blocked cell cycle arrest and protected cells from apoptosis. Taxol up-regulated the protein levels of P-HA, PARP, Chk1, p53, and p21 and down-regulated Bcl-Xl and Mcl-1, and RAP reversed the expression of all these proteins. These results suggested that RAP can protect immune cells from Taxol-induced toxicity, by changing the cell cycle and apoptosis.
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http://dx.doi.org/10.3389/fphar.2018.01580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378367PMC
February 2019

Pro-inflammatory role of transient receptor potential canonical channel 6 in the pathogenesis of chronic rhinosinusitis with nasal polyps.

Int Forum Allergy Rhinol 2018 11 14;8(11):1334-1341. Epub 2018 Sep 14.

Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Background: The pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP) has not been fully elucidated. This study sought to explore the role and mechanism of transient receptor potential canonical channel 6 (TRPC6) in the pathogenesis of CRSwNP.

Methods: Immunohistochemistry (IHC) was employed to evaluate TRPC6 immunolabeling. Real-time polymerase chain reaction (PCR) was conducted to assay TRPC6, stromal interaction molecule 1 (STIM1), and calcium release-activated calcium channel protein 1 (Orai1) messenger RNA (mRNA) levels in 70 patients with CRSwNP, including eosinophilic CRSwNP (ECRSwNP) or non-eosinophilic CRSwNP (nECRSwNP), and 28 control subjects. The concentrations of inflammatory mediators, including interleukin (IL)-1β, IL-5, and IL-25, were assayed by enzyme-linked immunosorbent assay (ELISA). In experiments on human nasal epithelial cell (HNEC) culture and stimulation, the mean fluorescence intensity (MFI) of intracellular Ca was assayed by flow cytometry. Western blotting, real-time PCR, and ELISA were also conducted to assess the effects and mechanisms of TRPC6 activator 1-oleoyl-2-acetyl-glycerol (OAG) and TRPC6 inhibitor 1-[2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl) propoxy]ethyl-1H-imidazole (SKF-96365) on HNECs.

Results: Upregulation of TRPC6, STIM1, and Orai1 levels was found in CRSwNP patients, particularly in those with ECRSwNP. TRPC6-positive cells correlated positively with the numbers of eosinophils and neutrophils, respectively. Moreover, TRPC6 mRNA was positively correlated with STIM1 and Orai1 mRNA levels. The concentrations of inflammatory mediators, including IL-1β, IL-5, and IL-25, were elevated in CRSwNP, especially in ECRSwNP. In cultured HNECs, TRPC6, STIM1, Orai1, Ca MFI levels, and inflammatory mediators were upregulated by lipopolysaccharide (LPS) and OAG but were inhibited by SKF-96365.

Conclusion: TRPC6 plays a pro-inflammatory role in the pathogenesis of CRSwNP via regulating Ca flow.
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http://dx.doi.org/10.1002/alr.22208DOI Listing
November 2018

Clival metastasis of renal clear cell carcinoma: Case report and literature review.

World J Clin Cases 2018 Sep;6(9):301-307

Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.

The clivus is an atypical metastatic site for renal clear cell carcinoma (RCCC). Here we report a 54 year old man with acute cavernous sinus syndrome. Brain magnetic resonance imaging identified a clival-based lesion with associated bony erosion. The patient underwent endoscopic endonasal biopsy and partial resection of the clival mass. Because histologic examination of the resected specimen resulted in a diagnosis of RCCC, contrast-enhanced computed tomography scan of the abdomen was performed and showed an enhanced left renal mass. The patient subsequently underwent laparoscopic left radical nephrectomy and gamma knife was planned for the residual clival lesion. We also retrospectively reviewed available published reports on clival metastases, specifically those from RCCC, since 1990.
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http://dx.doi.org/10.12998/wjcc.v6.i9.301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134277PMC
September 2018

Interference of Quercetin on Astragalus Polysaccharide-Induced Macrophage Activation.

Molecules 2018 06 28;23(7). Epub 2018 Jun 28.

School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.

Polysaccharides, which exert immunoregulatory effects, are becoming more and more popular as food supplements; however, certain components of ordinary foods could be reducing the polysaccharides beneficial effects. Quercetin, a flavonoid found in common fruits and vegetables, is one such component. This study investigated the effects of quercetin on Astragalus polysaccharide RAP induced-macrophage activation. The results show quercetin decreases the NO production and iNOS gene expression in RAW264.7 cells, and it inhibits the production of cytokines in RAW264.7 cells and peritoneal macrophages. Western blot analysis results suggest that quercetin inhibits the phosphorylation of Akt/mTORC1, MAPKs, and TBK1, but has no effect on NF-κB in RAP-induced RAW264.7 cells. Taken together, the results show that quercetin partly inhibits macrophage activation by the Astragalus polysaccharide RAP. This study demonstrates that quercetin-containing foods may interfere with the immune-enhancing effects of Astragalus polysaccharide RAP to a certain extent.
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http://dx.doi.org/10.3390/molecules23071563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100010PMC
June 2018

[Analysis of curative effect of reconstruction of annular ligament combined with internal fixation for the treatment of Monteggia fracture].

Zhongguo Gu Shang 2018 May;31(5):477-479

Huangshi TCM Hospital, Huangshi 435000, Hubei, China.

Objective: To investigate the curative effect of fascia reconstruction of annular ligament combined with internal fixation for the treatment of Monteggia fracture.

Methods: From December 2014 to October 2016, 30 cases with Monteggia fracture were treated by fascia reconstruction of annular ligament combined with internal fixation including 18 males and 12 females with an average age of 34.6 years old ranging from 6 to 50 years old. Elbow joint function were evaluated according to Mackay efficacy evaluation criteria.

Results: All 30 patients were followed up for an average of 12.5 months. No radial head re-dislocation occurred. Internal fixation of reconstructive plate of ulna were all bone healing. According to the evaluation standard of Mackay curative effect, 23 cases were excellent, 5 cases were good, and 2 cases were poor. There were 4 cases of mild elbow pain and no pain in the wrist joint. Functional recovery was the fastest and most satisfactory in the forearm. Eight cases had dysfunction of elbow extension with an average limitation of 9.1 degrees. Six cases delayed injury of radial nerve were recovered for half a year.

Conclusions: For the Monteggia fracture, fascia reconstruction of annular ligament combined with internal fixation can effectively restore the forearm rotation function, the recent effect is satisfactory, further observation is needed for the long-term effect.
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http://dx.doi.org/10.3969/j.issn.1003-0034.2018.05.015DOI Listing
May 2018

[Tumor necrosis factor-α and transforming growth factor-β balance liver stem cell differentiation in cholestatic cirrhosis].

Nan Fang Yi Ke Da Xue Xue Bao 2018 Apr;38(4):375-383

1Department of Hepatology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China; 2Laboratory of Stem Cell Biology and Therapy, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China. E-mail:

Objective: To investigate the changes of tumor necrosis factor-α (TNF-α) and transforming growth factor-β (TGF-β) in mice with cholestatic cirrhosis and their role in regulating the balance of liver stem cell differentiation.

Methods: Balb/c mice were subjected to bile duct ligation (BDL), and serum biochemical parameters were measured and hepatic histopathology was observed using HE staining to evaluate the modeling of cholestatic cirrhosis. Immunohistochemistry and Western blotting were used to detect the changes of TNF-α and TGF-β in the mice after modeling. Mouse embryonic hepatic stem cells (HP14-19) were treated with different concentrations of TNF-α and TGF-β, and the cell differentiation was assessed using Western blotting, real-time PCR, and PAS staining.

Results: The mice receiving BDL showed significantly increased blood biochemical parameters (P<0.05), and HE staining revealed obviously increased collagen fibers in the liver with significantly increased expressions of TNF-α and TGF-β (P<0.05). In HP14-19 cells, induction with TNF-α and TGF-β for 3 days did not cause significant changes in cell differentiation, but induction for 5 days resulted in significantly increases intensity of PAS staining in the cells. The cells induced with 20, 40, and 80 ng/mL TNF-α for 5 days exhibited a significantly stronger expression of cytokeratin 18 than cytokeratin 19 (P<0.05), while induction with 20, 40, and 80 ng/mL TGF-β produced opposite changes in cytokeratin 18 and cytokeratin 19 expressions. Further induction of the cells with TNF-α and TGF-β for 10 days, did not alter the expression patterns of cytokeratin 18 and cytokeratin 19 observed on day 5, but their protein expression levels and PAS staining intensity of the cells were enhanced and their mRNA expressions became lowered.

Conclusion: Common bile duct ligation can induce conditions simulating cholestatic cirrhosis in mice. TNF-α and TGF-β are elevated in cholestatic cirrhosis and play opposite roles in regulating the differentiation balance of liver stem cells: the former promotes the differentiation of liver stem cells into hepatocytes, while the latter promotes the cell differentiation into colangiocytes.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765666PMC
April 2018

Comprehensive comparison of polysaccharides from Ganoderma lucidum and G. sinense: chemical, antitumor, immunomodulating and gut-microbiota modulatory properties.

Sci Rep 2018 04 18;8(1):6172. Epub 2018 Apr 18.

School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.

Both Ganoderma lucidum (GL) and G. sinense (GS) are used as Lingzhi in China. Their functions are assumed to mainly derive from triterpenes and polysaccharides; however, the two species have very different triterpenes profiles, if this was the case, then the bioactivity of these two species should differ. Instead, could the polysaccharides be similar, contributing to the shared therapeutic basis? In this study, two main polysaccharide fractions from different batches of GL and GS were systematically compared by a series of chemical and biological experiments. The results showed that the polysaccharides from two species shared the same structural features in terms of mono-/oligo-saccharide profiles, molecular size, sugar linkages, and IR/NMR spectra. In addition, these polysaccharides showed similar tumor-suppressive activity in mice. Further study on RAW264.7 cells indicated that these polysaccharides exhibited similar inducing effects to macrophages, as evaluated in the phagocytosis function, NO/cytokines production, inhibition against the viability and migration of cancer cells. Mechanistic investigation revealed the identical activation via TLR-4 related MAPK/NF-κB signaling pathway and gut-microbiota modulatory effects. In summary, GL and GS polysaccharides presented similar chemical features, antitumor/immunomodulating activities and mechanism; this establishes polysaccharides as the active principles and supports the official use of both species as Lingzhi.
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http://dx.doi.org/10.1038/s41598-018-22885-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5906605PMC
April 2018

Experimental Investigation of Spectra of Dynamical Maps and their Relation to non-Markovianity.

Phys Rev Lett 2018 Feb;120(6):060406

CAS Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei, Anhui 230026, China.

The spectral theorem of von Neumann has been widely applied in various areas, such as the characteristic spectral lines of atoms. It has been recently proposed that dynamical evolution also possesses spectral lines. As the most intrinsic property of evolution, the behavior of these spectra can, in principle, exhibit almost every feature of this evolution, among which the most attractive topic is non-Markovianity, i.e., the memory effects during evolution. Here, we develop a method to detect these spectra, and moreover, we experimentally examine the relation between the spectral behavior and non-Markovianity by engineering the environment to prepare dynamical maps with different non-Markovian properties and then detecting the dynamical behavior of the spectral values. These spectra will lead to a witness for essential non-Markovianity. We also experimentally verify another simplified witness method for essential non-Markovianity. Interestingly, in both cases, we observe the sudden transition from essential non-Markovianity to something else. Our work shows the role of the spectra of evolution in the studies of non-Makovianity and provides the alternative methods to characterize non-Markovian behavior.
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http://dx.doi.org/10.1103/PhysRevLett.120.060406DOI Listing
February 2018

Are gravitational wave ringdown echoes always equal-interval?

Eur Phys J C Part Fields 2018 11;78(6):482. Epub 2018 Jun 11.

1School of Physics, University of Chinese Academy of Sciences, Beijing, 100049 China.

Gravitational wave (GW) ringdown waveforms may contain "echoes" that encode new physics in the strong gravity regime. It is commonly assumed that the new physics gives rise to the GW echoes whose intervals are constant. We point out that this assumption is not always applicable. In particular, if the post-merger object is initially a wormhole, which slowly pinches off and eventually collapses into a black hole, the late-time ringdown waveform exhibit a series of echoes whose intervals are increasing with time. We also assess how this affects the ability of Advanced LIGO/Virgo to detect these new signals.
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http://dx.doi.org/10.1140/epjc/s10052-018-5974-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424174PMC
June 2018

Evaluation of short-term effectiveness of eight targeted agents combined with chemotherapy for treating esophageal-gastric junction adenocarcinoma: A network meta-analysis.

J Cell Biochem 2018 01 5;119(1):1183-1192. Epub 2017 Oct 5.

Department of Integrated Traditional Chinese and Western Medicine, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, China.

This study aimed to evaluate the short-term effectiveness of eight targeted agents (ramucirumab, bevacizumab, rilotumumab, panitumumab, cetuximab, trebananib, trastuzumab, matuzumab) plus chemotherapy in esophageal-gastric junction adenocarcinoma (EGJA) by a network meta-analysis (NMA). PubMed, Embase, and Cochrane Library databases were systematically retrieved for randomized clinical trials (RCTs) concerning targeted agents plus chemotherapy in the treatment of EGJA. This NMA combined both direct and indirect evidence to evaluate odds ratio (OR) and to draw the surface under the cumulative ranking curve (SUCRA). In total 11 RCTs with 3649 EGJA patients (1907 patients treated with targeted agents plus chemotherapy were regarded as the case group, and 1742 patients with placebo plus chemotherapy were assigned into the control group) were enrolled in this study. Targeted agents in terms of stable disease (SD), partial response (PR), disease control rate (DCR), and overall response ratio (ORR) with the SUCRA values of 0.838, 0.807, 0.934, and 0.793, respectively. Cetuximab and trastuzumab, with the SUCRA values of 0.884 and 0.758, came on top as the best outcomes for treating EGJA in terms of progressive disease (PD) and complete response (CR). Cluster analysis results indicated that ramucirumab plus chemotherapy might be the optimal treatment for EGJA. Our findings indicated that ramucirumab plus chemotherapy might be the optimal treatment for EGJA amongst the nine treatment regimens, which provided clinical guidance for clinicians in the treatment of EGJA.
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http://dx.doi.org/10.1002/jcb.26288DOI Listing
January 2018

Anti-Fatigue Effects of the Unique Polysaccharide Marker of Dendrobium officinale on BALB/c Mice.

Molecules 2017 Jan 18;22(1). Epub 2017 Jan 18.

School of Chinese Medicine, Hong Kong Baptist University, 7 Baptist University Road, Kowloon Tong, Hong Kong, China.

extract shows potent anti-fatigue effects; however, the active substance responsible for these effects remains undetermined. A glucomannan with a huge molecular size of 730 kDa, called DOP, was identified as the unique authentication marker of this expensive herb. DOP exhibited immunomodulating effects on macrophages and lymphocytes in our previous study. Clinical reports also showed that people with fatigue syndrome have a disturbed immune system. Because DOP is the unique and dominant component of , we hypothesize that DOP may also have anti-fatigue activity. The present study aims to evaluate the anti-fatigue activity of DOP on BALB/c mice, with extract as a positive control. DOP and extract were orally administered at doses of 50 mg/kg and 100 mg/kg, respectively, for four weeks, and the anti-fatigue activity of DOP on BALB/c mice was evaluated using the weight-loaded swimming test. The contents of lactic dehydrogenase (LDH), creatine phosphokinase (CK), triglyceride (TG), blood urea nitrogen (BUN), superoxide dismutase (SOD), malondialdehyde (MDA), lactic acid (LD), and glutathione peroxidase (GSH-Px) in serum, glycogen of liver and gastrocnemius muscle were also determined. Their effects on variability of T cells and B cells were determined by using tetrazolium compound (MTS) method. The weight-loaded swimming exercise caused fatigue syndrome, mainly including the decreases of serum SOD/GSH-Px and gastrocnemius glycogen, as well as the increases of LDH, BUN, MDA, CK, TG, and LD in serum. All of these indicators of fatigue were inhibited to a certain extent by both DOP and extract; however, the effects of DOP were much stronger than those of extract. Compared to the positive control, mice dosed with DOP showed increases in endurance, body weight, and food intake. Furthermore, DOP-feeding mice significantly increased the cell variability of T lymphocytes and B lymphocytes, compared with that of mice in control group. This study indicates that the unique and dominant polysaccharide DOP of has stronger anti-fatigue activity than extract. As such, DOP has promising potential for pharmaceutical development into health products to reduce fatigue.
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http://dx.doi.org/10.3390/molecules22010155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155575PMC
January 2017
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