Publications by authors named "Zhi-Hui Xu"

23 Publications

  • Page 1 of 1

Roxadustat treatment for anemia in peritoneal dialysis patients: A randomized controlled trial.

J Formos Med Assoc 2021 Jun 21. Epub 2021 Jun 21.

Department of Nephrology, 2nd Affiliated Hospital of Harbin Medical University, Harbin, China; Department of Nephrology, Institute of Nephrology, 2nd Affiliated Hospital of Hainan Medical University, Haikou, China. Electronic address:

Background/purpose: Roxadustat, a first-in-class hypoxia-inducible factor prolyl hydroxylase inhibitor, promotes erythropoiesis and regulates iron metabolism. This study investigated the efficacy and safety of roxadustat in Chinese patients with anemia on peritoneal dialysis (PD).

Methods: One hundred and twenty-nine patients were randomized and treated with roxadustat (n = 86) or erythropoiesis-stimulating agents (ESAs) (n = 43) for 24 weeks. The primary end points were the mean hemoglobin (Hb) level at week 24, the change in average Hb levels from baseline to week 24, and the cumulative response rate throughout the treatment period. The secondary end points included changes in hepcidin and iron indices and serum lipid levels. Subgroup analysis examined the effect of inflammatory status on the efficacy of Hb. Safety was assessed as the occurrence of emergent adverse events after treatment.

Results: The mean average Hb levels at week 24 and average change in Hb levels from baseline to week 24 were 11.5 g/dL and 2.5 g/dL in the roxadustat group and 11.2 g/dL and 2.2 g/dL in the ESAs group, respectively. The cumulative response rate was 96% in the roxadustat group and 92% in the ESAs group at week 24. Roxadustat decreased hepcidin levels and increased total iron-binding capacity. The decreases in total cholesterol and low-density lipoprotein cholesterol were greater with roxadustat than with ESAs. Roxadustat-induced Hb increases were independent of baseline C-reactive protein levels. Common adverse events included hyperkalemia, hypertension, and insomnia.

Conclusion: Roxadustat effectively corrected and maintained target Hb levels in Chinese PD patients. This trial was registered in the Chinese Clinical Trial Register (ChiCTR2000035054).
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http://dx.doi.org/10.1016/j.jfma.2021.06.004DOI Listing
June 2021

Clinical outcomes of sacral neuromodulation in non-neurogenic, non-obstructive dysuria: A 5-year retrospective, multicentre study in China.

World J Clin Cases 2020 Jun;8(12):2494-2501

Department of Urology, West China Hospital, Sichuan University, Chengdu 610000, Sichuan Province, China.

Background: Management of non-neurogenic, non-obstructive dysuria represents one of the most challenging dilemmas in urological practice. The main clinical symptom is the increase in residual urine. Voiding dysfunction is the main cause of dysuria or urinary retention, mainly due to the decrease in bladder contraction (the decrease in contraction amplitude or duration) or the increase in outflow tract resistance. Sacral neuromodulation (SNM) has been used for > 10 years to treat many kinds of lower urinary tract dysfunction. It has become increasingly popular in China in recent years. Consequently, studies focusing on non-neurogenic, non-obstructive dysuria patients treated by SNM are highly desirable.

Aim: To assess the outcome of two-stage SNM in non-neurogenic, non-obstructive dysuria.

Methods: Clinical data of 54 patients (26 men, 28 women) with non-neurogenic, non-obstructive dysuria treated by SNM from January 2012 to December 2016 in ten medical centers in China were retrospectively analyzed. All patients received two or more conservative treatments. The voiding diary, urgency score, and quality of life score before operation, after implantation of tined lead in stage I (test period), and during short-term follow-up (latest follow-up) after implantation of the implanted pulse generator in stage II were compared to observe symptom improvements.

Results: Among the 54 study patients, eight refused to implant an implanted pulse generator because of the unsatisfactory effect, and 46 chose to embed the implanted pulse generator at the end of stage I. The conversion rate of stage I to stage II was 85.2%. The average follow-up time was 18.6 mo. There were significant differences between baseline (before stage I) and the test period (after stage I) in residual urine, voiding frequency, average voiding amount, maximum voiding amount, nocturia, urgency score, and quality of life score. The residual urine and urgency score between the test period and the latest follow-up time (after stage II) were also significantly different. No significant differences were observed for other parameters. No wound infection, electrode breakage, or other irreversible adverse events occurred.

Conclusion: SNM is effective for patients with non-neurogenic, non-obstructive dysuria showing a poor response to traditional treatment. The duration of continuous stimulation may be positively correlated with the improvement of residual urine.
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http://dx.doi.org/10.12998/wjcc.v8.i12.2494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322430PMC
June 2020

[Effects of Different Concentrations of Ammonia Nitrogen on the Growth and Enzyme Activity of Four Common Algae Strains].

Huan Jing Ke Xue 2019 Aug;40(8):3642-3649

College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China.

Treating swine wastewater with a high ammonia nitrogen content with microalgae cultures has proved difficult. In this paper, the strains , , , and were tested. Ammonia nitrogen concentrations of 50 mg·L, 500 mg·L, and 2000 mg·L applied to the media according to the concentrations of biogas slurry. This allowed the effect of different concentrations of ammonia nitrogen on the growth and cell enzyme activity of microalgae to be tested. The results showed that the growth of 715 and was inhibited at different concentrations of ammonia nitrogen, and the biomass and biomass productivities were lower than for the normal media. However, the biomass and biomass productivity of in 50 mg·L ammonia nitrogen were 1.78 g·L and 0.16 g·(L·d), respectively, which were higher than the values obtained using KOCK medium. Furthermore, the biomass and biomass productivity of 357 in 500 mg·L ammonia nitrogen were 1.95 g·L and 0.18 g·(L·d), respectively, which were higher than the values obtained using BG11 medium. The SOD, POD, and CAT of all algae species showed a decreasing tendency in response to an increase in the concentration of ammonia nitrogen, as did MDA. These results provide a theoretical basis for the treatment of swine wastewater with high ammonia nitrogen content using microalgae cultures.
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http://dx.doi.org/10.13227/j.hjkx.201811224DOI Listing
August 2019

Results of Sacral Neuromodulation Therapy for Urinary Voiding Dysfunction: Five-Year Experience of a Retrospective, Multicenter Study in China.

Neuromodulation 2019 Aug 4;22(6):730-737. Epub 2019 Jan 4.

Department of Urology, West China Hospital, Sichuan University, Chengdu, P.R. China.

Purpose: This five-year, retrospective, multicenter study evaluated the long-term safety and efficiency of sacral neuromodulation (SNM) in Chinese patients with urinary voiding dysfunction.

Patients And Methods: This is a Chinese national, multicenter, retrospective study that included 247 patients (51.2% female) who received an implantable pulse generator (IPG) (InterStim, Medtronic, Minneapolis, MN, USA) between 2012 and 2016. Success was considered if the initial ≥50% improvement in any of primary voiding diary variables persisted compared with baseline. The results were further stratified by identifying patients who showed >50% improvement and those although showed <50% improvement but still wanted to receive IPG; these data were collected and analyzed for general improvement.

Results: Following test stimulation, 187 patients (43%) declined implantation and 247 (57%) underwent implantation using InterStim®. Among 247 patients, 34 (13.7%) had overactive bladder (OAB), 59 (23.8%) had interstitial cystitis/bladder pain syndrome (IC/BPS), 47 (19%) had idiopathic urinary retention (IUR), and 107 (44.1%) had neurogenic bladder (NB). IPG efficiency rate for OAB, interstitial cystitis/bladder pain syndrome, idiopathic urinary retention, and neurogenic bladder were 42.5, 72.4, 51.6, and 58.8%, respectively. The mean duration of follow-up was 20.1 ± 12.8 months.

Conclusions: SNM appears effective in the long term, with a total IPG implantation rate of approximately 57% (ranging between 42.5 and 72.4% depending on indication). Interstitial cystitis/bladder pain syndrome appear to be the best indication for stage I testing. Chinese neurogenic bladder patients are most inclined to choose SNM. SNM is relatively safe, with low postoperation adverse events of 16.1% and reoperation rate of 3.2% during the follow-up period.
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http://dx.doi.org/10.1111/ner.12902DOI Listing
August 2019

[Efficacy of Huang'e Capsules on benign prostatic hyperplasia with Qi-deficiency, blood stasis and damp heat block: A stratified analysis].

Zhonghua Nan Ke Xue 2018 Sep;24(9):819-823

Department of Andrology, Guanganmen Hospital of the China Academy of Chinese Medical Sciences, Beijing 100053, China.

Objective: To evaluate the efficacy and safety of Huang'e Capsules in the treatment of BPH with Qi-deficiency, blood stasis and damp heat block.

Methods: This study included 1 456 cases of BPH with Qi-deficiency, blood stasis and damp heat block from 40 hospitals of Zhejiang and Anhui Provinces between June 2014 and July 2017. The patients were aged 40-85 years and treated with Huang'e Capsules at a dose of 4 capsules, tid, for a course of 6 weeks. The IPSSs of the patients were obtained before and after 2, 4 and 6 weeks of medication, and a stratified analysis was made on the factors influencing the therapeutic effects, such as age, the stage of BPH, and concomitant medication of urogenital and reproductive hormone drugs.

Results: After 6 weeks of medication, the IPSS of the patients was decreased by 8.4 ± 5.4 ([42.9 ± 22.7] %) as compared with the baseline (19.2 ± 6.8) (P < 0.05), with a total effectiveness rate of 71.9% and an excellence rate of 23.1%. After 6 weeks of medication, the IPSSs of the patients with stage-Ⅰ, -Ⅱ and -Ⅲ BPH were decreased by 7.3 ± 6.8, 6.6 ± 4.3 and 11.0 ± 5.5, with total effective rates of 69.5%, 70.1% and 74.7%, respectively, with statistically significant differences among the three stages (P < 0.05), and so were those in the patients aged ≤50 years (by 7.4 ± 5.8), 51-60 years (by 7.9 ± 5.3), 61-70 years (by 8.7 ± 5.6) and >70 years (by 8.6 ± 5.1), but with no statistically significant differences among different age groups (P > 0.05). At 6 weeks, the IPSS was reduced by 7.1 ± 5.9 by concomitant medication of urogenital and reproductive hormone drugs, 8.7 ± 5.4 by concomitant medication of other drugs, and 8.4 ± 5.3 by non-concomitant medication, with no statistically significant differences among the three types of medication (P > 0.05).

Conclusions: Huang'e Capsules can evidently improve the symptoms of BPH, with the best effect on stage-Ⅲ BPH, and the effect does not differ significantly with age or drug concomitance.
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September 2018

[Hypospadias induced by maternal exposure to di-n-butyl phthalate and its mechanisms in male rat offspring , , , , ,].

Zhonghua Nan Ke Xue 2017 Dec;23(12):1063-1068

Department of Urology, Zhejiang Provincial People's Hospital/People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, China.

Objective: To induce hypospadias in male rat offspring by maternal exposure to di-n-butyl phthalate (DBP) during late pregnancy and further investigate its mechanisms.

Methods: We randomly divided 20 pregnant rats into a DBP exposure and a control group, the former treated intragastrically with DBP while the latter with soybean oil at 750 mg per kilogram of the body weight per day from gestation days (GD) 14 to 18. On postnatal day (PND) 1, we recorded the incidence rate of hypospadias and observed the histopathological changes in the genital tubercle of the hypospadiac rats. We also measured the level of serum testosterone (T) by radioimmunoassay and determined the mRNA and protein expressions of the androgen receptor (AR), sonic hedgehog (Shh), bone morphogenetic protein 4 (Bmp4) and fibroblast growth factor 8 (Fgf8) in the genital tubercle by real-time PCR and Western blot.

Results: No hypospadiac male rats were found in the control group. The incidence rate of hypospadias in male offspring was 43.6% in the DBP-treatment group. Histological analysis confirmed hypospadiac malformation. The serum testosterone concentration was decreased in the hypospadiac male rats as compared with the controls ([0.49 ± 0.05] vs [1.12 ± 0.05] ng/ml, P <0.05). The mRNA expressions of AR, Shh, Bmp4 and Fgf8 in the genital tubercle were significantly lower in the hypospadiac male rats than in the controls (AR: 0.50 ± 0.05 vs 1.00 ± 0.12, P <0.05; Shh: 0.65 ± 0.07 vs 1.00 ± 0.15, P <0.05; Bmp4: 0.42 ± 0.05 vs 1.00 ± 0.13, P <0.05; Fgf8: 0.46 ± 0.04 vs 1.00 ± 0.12, P <0.05), and so were their protein expressions (AR: 0.34 ± 0.05 vs 1.00 ± 0.09, P <0.05; Shh: 0.51 ± 0.07 vs 1.00 ± 0.12, P <0.05; Bmp4: 0.43 ± 0.05 vs 1.00 ± 0.11, P <0.05; Fgf8: 0.57 ± 0.04 vs 1.00 ± 0.13, P <0.05).

Conclusions: Maternal exposure to DBP during late pregnancy can induce hypospadias in the male rat offspring. DBP affects the development of the genital tubercle by reducing the serum T concentration and expressions of AR, Shh, Bmp4 and Fgf8 in the genital tubercle, which might underlie the mechanism of DBP inducing hypospadias.
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December 2017

In situ observation of fracture behavior of canine cortical bone under bending.

Mater Sci Eng C Mater Biol Appl 2016 May 28;62:361-7. Epub 2016 Jan 28.

Department of Mechanical Engineering, University of South Carolina, 300 Main Street, Columbia, SC 29208, United States; Department of Mechanical and Aerospace Engineering, University of Virginia, 122 Engineer's Way, Charlottesville, VA 22904, United States. Electronic address:

Cortical bone provides many important body functions and maintains the rigidness and elasticity of bone. A common failure mode for bone structure is fracture under a bending force. In the current study, the fracture behavior of canine cortical bone under three-point bending was observed in situ using an atomic force microscope (AFM), a scanning electron microscope (SEM), and an optical microscope to examine the fracture process in detail. Nanoindentation was carried out to determine the elastic modulus and hardness of different building blocks of the canine cortical bone. The results have shown that the special structure of Haversian systems has significant effects on directing crack propagation. Although Haversian systems contain previously believed weak points, and micro-cracks initiate within Haversian systems, our findings have demonstrated that macro-cracks typically form around the boundaries of Haversian systems, i.e. the cement lines. Micro-cracks that developed inside Haversian systems have the functions of absorbing and dissipating energy and slow down on expanding when interstitial tissue cannot hold any more pressure, then plastic deformation and fracture occur.
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http://dx.doi.org/10.1016/j.msec.2016.01.061DOI Listing
May 2016

[Biosynthetic schwertmannite as catalyst in Fenton-like reactions for degradation of methyl orange].

Huan Jing Ke Xue 2015 Mar;36(3):995-9

Biosynthesized schwertmannite was used as catalyst in photo-Fenton-like reaction to facilitate the degradation of methyl orange (MO). Schwertmannite was synthesized through the oxidation of FeSO4 by Acidithiobacillus ferrooxidans LX5 cell suspension at an initial pH 2.5 and 28 degress C for 3 days and characterized using X-ray diffraction spectroscopy and scanning electron microscope. The oxidative degradation of MO in the photo-Fenton-like reaction was studied at different initial pH values of suspension, concentrations of H2O2 and dosages of catalyst. The results suggested that the biosynthetic schwertmannite showed a good catalytic activity in the MO degradation via *OH radical mechanism. Considerable degradation efficiency of MO was still obtained in approximately neutral condition or in the presence of high concentrations of chloride, sulfate and nitrate. This work demonstrated that the heterogeneous photo-Fenton-like reaction catalyzed by the biosynthetic schwertmannite is a promising advanced oxidation technology for the treatment of wastewater containing MO.
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March 2015

Complete mitochondrial genome sequence and mutations of the laryngeal cancer model inbred nude rat strain.

Mitochondrial DNA A DNA Mapp Seq Anal 2016 11 29;27(6):4185-4186. Epub 2015 Jan 29.

a The Second Clinical Medical College of Fujian Medical University , Quanzhou , China.

In the present work, we undertook the complete mitochondrial genome sequencing of an important laryngeal cancer model inbred rat strain for the first time. The total length of the mitogenome was 16,308 bp. It harbored 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and one non-coding control region (D-loop region). The mutation events were also reported.
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http://dx.doi.org/10.3109/19401736.2014.1003904DOI Listing
November 2016

[Photochemical degradation of landfill leachate facilitated by combined schwertmannite and H2O2].

Huan Jing Ke Xue 2014 Apr;35(4):1407-13

It is practically important that high concentrations of organic pollutants in landfill leachate were degraded by a rapid and efficient approach. The influence of operating conditions such as schwertmannite dosage, V(H2O2)/m (schwertmannite) ratio on the degradation efficiency of color, TOC and COD contents of landfill leachate, was investigated by using the schwertmannite/H2O2/UV process. It was demonstrated that the color, TOC and COD removal efficiencies increased significantly with the increase in schwertmannite dosage, and then were approximately stable. However, COD removal efficiency declined because of the presence of the residual H2O2 when V (H2O2)/m (schwertmannite) was greater than 2, and the best removal efficiency of COD was 44.9%. Furthermore, high-intensity ultraviolet was more conducive to eliminate pollutants through photochemical oxidation with schwertmannite/H2O2. The color, TOC and COD removal efficiencies were 90.0%, 78.8% and 52.6% respectively after 2.5 hours of photochemical degradation, with UV-500 W under optimal initial pH = 2.5; meanwhile, this study found that it was beneficial to the photochemical degradation of leachate at room temperature via the schwertmannite/H2O2/UV process, and COD removal efficiency declined gradually when the temperature was higher than 25 degrees C. Controlled trials showed that the schwertmannite/H2O2 method was conducive to the removal of color compared with the traditional homogeneous Fenton reaction.
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April 2014

Multiscale hierarchical assembly strategy and mechanical prowess in conch shells (Busycon carica).

J Struct Biol 2013 Dec 30;184(3):409-16. Epub 2013 Oct 30.

Department of Mechanical Engineering, University of South Carolina, 300 Main Street, Columbia, SC 29208, USA.

Seashells are natural body armors with superior mechanical strength and ultra-high toughness compared with their major constituent counterparts. What building blocks and architecture render seashells such mechanical prowess? In this study, micro/nanoscale structural and mechanical characterization of conch shells (Busycon carica) has been carried out. Here we show direct evidence that the previously claimed single-crystal third-order lamellae--the basic building blocks in conch shells are essentially assembled with aragonite nanoparticles of the size ranging from 20 to 45 nm. The nanoparticle-constructed third-order lamellae are not brittle, but ductile. The three-order crossed-lamellar architecture interlocks cracks via crack deflection along the interfaces in a three-dimensional manner, thus confining the damage in a small region. The findings advance the understanding of the mystery of conch shell's mechanical robustness, provide additional design guidelines for developing bioinspired nanomaterials, and lay a constitutive foundation for modeling the deformation behavior of seashells.
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http://dx.doi.org/10.1016/j.jsb.2013.10.011DOI Listing
December 2013

[Five patients with Madelung's disease].

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2012 Jan;47(1):70-1

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January 2012

Characterization of hepatitis B virus genotypes/subgenotypes in 1,301 patients with chronic hepatitis B in North China.

Chin Med J (Engl) 2011 Dec;124(24):4178-83

Institute of Infectious Diseases and Liver Failure Research Center, Beijing 302 Hospital, Beijing 100039, China.

Background: There is still a paucity of data on hepatitis B virus (HBV) subgenotype prevalence in North China based on sequencing of large-size samples. In addition, whether HBV genotypes impact drug-resistance-associated and HBV e antigen (HBeAg)-loss-associated mutations in patients with chronic hepatitis B (CHB) is still under investigation. This study aimed to disclose clinical prevalence of HBV genotypes/subgenotypes in North China and the clinical implications of HBV genotype classification in respect to HBeAg loss and drug-resistant occurrence.

Methods: Sera were collected from 1301 nucleos(t)ide analog-experienced CHB patients. Viral DNA was extracted and used as template for HBV genome amplification by nested PCR. DNA sequencing was performed for the analysis of HBV genotypes/subgenotypes, drug-resistance-associated mutations in polymerase gene and HBeAg-loss-associated mutations in precore/basal core promoter (BCP) regions.

Results: HBV/B, HBV/C, and HBV/D were detected in 190 (14.6%), 1096 (84.2%), and 15 (1.2%) patients, respectively. HBV/B2 (182/190), HBV/C2 (1069/1096), and HBV/D1 (12/15) were predominant subgenotypes within individual genotypes. By contrast, C2 prevalence is relatively lower in Beijing area (77.2%) than in other north areas (84.9% - 87.4%). HBV/C-infected patients had an older age and a lower serum albumin level but similar HBV DNA and alanine aminotransferase (ALT) levels compared to HBV/B-infected patients. HBV/C infection had a higher incidence of lamivudine-resistant mutations rtM204I/V (44.9% vs. 30.2%, P < 0.01) and BCP mutations A1762T+G1764A (65.8% vs. 40.0%, P < 0.01) compared with HBV/B infection.

Conclusions: C2 is the most prevalent HBV subgenotype followed by B2 in CHB patients in North China; and HBV genotype prevalence is influenced by immigrant population. HBV/C infection is likely to have longer disease duration and severer liver functional impairment and might be more susceptible to develop lamivudine resistance compared to HBV/B infection.
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December 2011

Hydrogen passivation induced dispersion of multi-walled carbon nanotubes.

Adv Mater 2012 Feb 17;24(7):881-5. Epub 2012 Jan 17.

Department of Mechanical Engineering, University of South Carolina, 300 Main Street, Columbia, SC 29208, USA.

A method combining hydrogen passivation and ultrasonication was developed for the first time to disperse multi-walled carbon nanotubes (MWCNTs) in ethanol solution and epoxy resin. Excellent dispersion of MWCNTs was achieved in both media. Three-point bending tests of the MWCNT/epoxy nanocomposites revealed a remarkable increase in elastic modulus with increasing MWCNT content.
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http://dx.doi.org/10.1002/adma.201104337DOI Listing
February 2012

Electrical self-healing of mechanically damaged zinc oxide nanobelts.

Nano Lett 2011 Jan 1;11(1):241-4. Epub 2010 Dec 1.

Department of Mechanical Engineering, University of South Carolina, 300 Main Street, Columbia, South Carolina 29208, United States.

We report the observation of remarkable electrical self-healing in mechanically damaged ZnO nanobelts. Nanoindentation into intrinsically defect-free ZnO nanobelts induces deformation and crack damage, causing a dramatic electrical signal decrease. Two self-healing regimes in the nanoindented ZnO nanobelts are revealed. The physical mechanism for the observed phenomena is analyzed in terms of the nanoindentation-induced dislocations, the short-range atomic diffusion in nanostructures, and the local heating of the dislocation zone in the electrical measurement.
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http://dx.doi.org/10.1021/nl103637kDOI Listing
January 2011

Catalyst-free synthesis and structural and mechanical characterization of single crystalline Ca2B2O5.H2O nanobelts and stacking faulted Ca2B2O5 nanogrooves.

Nano Lett 2010 Jan;10(1):255-62

Department of Mechanical Engineering, University of South Carolina, 300 Main Street, Columbia, SC 29208, USA.

Single crystalline Ca(2)B(2)O(5).H(2)O (CBOH) nanobelts were synthesized for the first time using a facile catalyst-free hydrothermal method at low temperature. After being annealed at 400 degrees C for 10 h, the CBOH nanobelts transformed into curve-shaped Ca(2)B(2)O(5)(CBO) nanogrooves with ordered stacking fault arrays along the [110] direction. The single crystalline CBOH nanobelts and the stacking faulted CBO nanogrooves provided a unique platform for investigating the effects of hydrated H(2)O and stacking faults on the mechanical properties of nanomaterials. Nanoindentation tests were performed directly on individual CBOH nanobelts and CBO nanogrooves to probe their mechanical properties. It was found that the CBOH nanobelts, with the presence of hydrated H(2)O, achieved 28.7% increase in elastic modulus, whereas the stacking faulted CBO nanogrooves, with the absence of hydrated H(2)O, exhibited 99% loss in elastic modulus, compared to bulk CBO.
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http://dx.doi.org/10.1021/nl9034187DOI Listing
January 2010

Hepatitis B virus in tenofovir-naive Chinese patients with chronic hepatitis B contains no mutation of rtA194T conferring a reduced tenofovir susceptibility.

Chin Med J (Engl) 2009 Jul;122(13):1585-6

Viral Hepatitis Research Laboratory, Institute of Infectious Diseases, Beijing 302 Hospital, Beijing 100039, China.

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July 2009

[Transurethral unroofing in seminal vesicle cyst].

Zhonghua Yi Xue Za Zhi 2009 Mar;89(8):552-4

Department of Urology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.

Objective: To investigate the effects of transurethral unroofing in treatment of seminal vesicle cyst.

Methods: Seven patients seminal vesicle cyst 2.1 cm x 3.0 cm x 3.3 cm - 5.5 cm x 6.3 cm x 10.2 cm in size, aged 36 (21 - 65), with the symptoms of hematospermia (4 cases), hematuria (3 cases), epididymitis (2 cases), dysuria (1 case), bladder irritation symptoms (1 case), bloody mucus of urethral orifice after miction (1 case), perineal discomfort (2 case), and infertility (3 cases), underwent transurethral unroofing of seminal vesicle cyst.

Results: Operation was performed successful on all 7 patients. The mean operative time was 20 min, the mean estimated operative blood loss was 5 ml, and the mean hospital stay was 3 d. Follow-up was conduced for 28.3 months (8 months-10 years). All patients were free of symptoms after surgery, without recurrence and complications that needed further therapy. Two of the 3 patients with infertility succeeded in fertilization 1 year after surgery, and 1 patient with retrograde ejaculation underwent intracytoplasmic sperm injection and succeeded in fertilization 6 months after operation.

Conclusion: Transurethral unroofing provides an effective approach to the seminal vesicle cyst with minimal invasion, short hospitalization, and rapid recovery.
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March 2009

[Quantitative detection of hepatitis B virus covalently closed circular DNA in sera of chronic hepatitis B patients with a newly established assay].

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2008 Jun;22(3):225-7

Viral Hepatitis Research Laboratary, Institute of Infectious Disease of PL4 302 Hospital, Beijing 100039, China.

Objective: To quantitatively detect hepatitis B virus covalently closed circular DNA (HBV cccDNA) in sera of chronic hepatitis B patients with a newly established assay.

Methods: Primers and probe were designed in highly conservative region of HBV DNA. DNA was extracted from 175 sera samples of chronic hepatitis B patients, and was treated with plasmid-Safe-ATP-dependent Dnase(PSAD) to eliminate the relaxed circular DNA (rcDNA). The products were amplified by real-time PCR with primers spanning.

Results: The detection rate of serum HBV cccDNA was found to correlate directly with serum HBV DNA loading. HBeAg positive chronic hepatitis B patients had higher serum HBV cccDNA levels than HBeAg negative chronic hepatitis B patients.

Conclusion: The method is good because of the high specificity. It can be used for detection of HBV cccDNA. DNA;
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June 2008

[Multiple-site analysis of HBV drug-resistant mutations in 340 patients with chronic hepatitis B].

Zhonghua Gan Zang Bing Za Zhi 2008 Oct;16(10):735-8

Viral Hepatitis Research Laboratory, Institute of Infectious Diseases, 302 Hospital of PLA, Beijing 100039, China.

Objectives: To analyze HBV drug-resistant mutations against nucleos(t)ide analogues at 12 reported sites in 340 patients with chronic hepatitis B.

Methods: Serum HBV DNA was extracted and a nested PCR assay was employed for the reverse transcriptase (RT) gene amplification. Direct sequencing of PCR product was performed. The significance of detected mutations was analyzed in view of clinical data of the patients.

Results: Drug-resistant mutations were detected in 68 patients taking lamivudine (LAM), 10 taking adefovir (ADV), 8 taking entecavir, and 1 taking telbivudine (LdT). M204V and M204I were the most common LAM-resistant mutations. The former usually emerged with L180M while the latter often emerged alone. N236T +/- A181 substitution was the most frequently seen ADV-resistant mutation. ETV-resistant mutations occurred on the basis of LAM-resistant mutations and T184 change was the most common form. LdT-resistance was observed as M204I. Interestingly, these drug-resistant mutations were detected in a few patients who had not been treated with nucleos(t)ide analogues.

Conclusion: Detection of HBV drug-resistant mutations at multiple sites of the viral RT gene is valuable for discovering and verifying drug resistance and thus is very helpful in planning anti-HBV therapy.
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October 2008

Friction and wear behavior of ultra-high molecular weight polyethylene as a function of polymer crystallinity.

Acta Biomater 2008 Sep 13;4(5):1401-10. Epub 2008 Mar 13.

Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA.

In this study the friction, wear and surface mechanical behavior of medical-grade ultra-high molecular weight polyethylene (UHMWPE) (GUR 1050 resin) were evaluated as a function of polymer crystallinity. Crystallinity was controlled by heating UHMWPE to a temperature above its melting point and varying the hold time and cooling rates. The degree of crystallinity of the samples was evaluated using differential scanning calorimetry (DSC). A higher degree of crystallinity in the UHMWPE resulted in lower friction force and an increase in scratch resistance at the micro- and nanoscales. On the nanoscale, the lamellar structure appeared to affect the observed wear resistance. Reciprocating-wear tests performed using a microtribometer showed that an increase in crystallinity also resulted in lower wear depth and width. Nanoindentation experiments also showed an increase in hardness values with an increase in sample crystallinity.
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http://dx.doi.org/10.1016/j.actbio.2008.02.022DOI Listing
September 2008

[Determination of astragulin in Thesium chinese by RP-HPLC].

Zhongguo Zhong Yao Za Zhi 2006 Nov;31(21):1766-7

School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China.

Objective: To establish a determination method of astragulin in Thesium chinese.

Method: RP-HPLC analytical method was established using a Polaris C18 column and acetonitrile-water (23:77) as the mobile phase, with a flow rate of 10 mL x min(-1), detected at 346 nm. The method of sample is refluxing exation by 50% alcohol for 2 times.

Result: The content of astragulin was from 0. 120% to 0. 155%, in different groups of T. chinese collected from the same location.

Conclusion: The method was validated to show convenient and reliable.
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November 2006

In situ observation of nanograin rotation and deformation in nacre.

Nano Lett 2006 Oct;6(10):2301-4

Department of Mechanical Engineering, University of South Carolina, 300 Main Street, Columbia, South Carolina 29208, USA.

Nacre is a natural nanocomposite material with superior mechanical strength and toughness. What roles do the nanoscale structures play in the inelasticity and toughening of nacre? Can we learn from this to produce nacre-like nanocomposites? Here we report in situ dynamic atomic force microscope observations of nacre with aragonite nanograins (nanoparticles) of an average grain size of 32 nm, which show that nanograin rotation and deformation are the two prominent mechanisms contributing to energy dissipation in nacre. The biopolymer spacing between the nanograins facilitates the grain rotation process. The aragonite nanograins in nacre are not brittle but deformable.
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http://dx.doi.org/10.1021/nl061775uDOI Listing
October 2006
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