Publications by authors named "Zhi-Gang Wang"

302 Publications

LncRNA HCP5 in hBMSC-derived exosomes alleviates myocardial ischemia reperfusion injury by sponging miR-497 to activate IGF1/PI3K/AKT pathway.

Int J Cardiol 2021 Jul 23. Epub 2021 Jul 23.

Department of Cardiothoracic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210000, Jiangsu Province, PR China. Electronic address:

Ischemia/reperfusion (I/R) injury is an inevitable process during heart transplant and suppressing I/R injury could greatly improve the survival rate of recipients. Mesenchymal stem cells (MSCs) have positive effects on I/R. We aimed to investigate the mechanisms underlying the protective roles of MSCs in I/R. Both cell model and rat model of myocardial I/R were used. MTT assay and flow cytometry were used to measure cell viability and apoptosis, respectively. QRT-PCR and western blotting were employed to measure levels of lncRNA HCP5 (HLA complex P5), miR-497, apoptosis-related proteins, and insulin-like growth factor (IGF1)/PI3K/AKT pathway. Dual luciferase assay was used to validate interactions of HCP5 and miR-497, miR-497 and IGF1. Echocardiography was performed to evaluate cardiac function of rats. Serum levels of CK-MB and LDH were measured. H&E and Masson staining were used to examine morphology of myocardial tissues. hBMSC-derived exosomes (hBMSC-Exos) increased the viability of cardiomyocytes following hypoxia/reperfusion (H/R) and decreased apoptosis. H/R diminished HCP5 expression in cardiomyocytes while hBMSC-Exos recovered the level. Overexpression of HCP5 in hBMSC-Exos further enhanced the protective effects in H/R while HCP5 knockdown suppressed. HCP5 directly bound miR-497 and miR-497 targeted IGF1. miR-497 mimics or si-IGF1 blocked the effects of HCP5 overexpression. Further, hBMSC-Exos alleviated I/R injury in vivo and knockdown of HCP5 in hBMSC-Exos decreased the beneficial effects. AntagomiR-497 blocked the effects of HCP5 knockdown. HCP5 from hBMSC-Exos protects cardiomyocytes against I/R injury via sponging miR-497 to disinhibit IGF1/PI3K/AKT pathway. These results shed light on mechanisms underlying the protective role of hBMSC-Exos in I/R.
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http://dx.doi.org/10.1016/j.ijcard.2021.07.042DOI Listing
July 2021

Disintegrating spatial networks based on region centrality.

Chaos 2021 Jun;31(6):061101

International Academic Center of Complex Systems, Beijing Normal University, Zhuhai 519087, People's Republic of China.

Finding an optimal strategy at a minimum cost to efficiently disintegrate a harmful network into isolated components is an important and interesting problem, with applications in particular to anti-terrorism measures and epidemic control. This paper focuses on optimal disintegration strategies for spatial networks, aiming to find an appropriate set of nodes or links whose removal would result in maximal network fragmentation. We refer to the sum of the degree of nodes and the number of links in a specific region as region centrality. This metric provides a comprehensive account of both topological properties and geographic structure. Numerical experiments on both synthetic and real-world networks demonstrate that the strategy is significantly superior to conventional methods in terms of both effectiveness and efficiency. Moreover, our strategy tends to cover those nodes close to the average degree of the network rather than concentrating on nodes with higher centrality.
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http://dx.doi.org/10.1063/5.0046731DOI Listing
June 2021

Quantum Dots: A Promising Fluorescent Label for Probing Virus Trafficking.

Acc Chem Res 2021 Jul 28;54(14):2991-3002. Epub 2021 Jun 28.

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Frontiers Science Center for New Organic Matter, Research Center for Analytical Sciences, College of Chemistry, and School of Medicine, Nankai University, Tianjin 300071, P. R. China.

ConspectusRecent research has highlighted the immense potential of the quantum dot (QD)-based single-virus tracking (SVT) technique in virology. In these experiments, the infection behaviors of single viruses or viral components, labeled with QDs, could be tracked on time scales of milliseconds to hours in host cells. The trajectories of individual viruses are reconstructed with nanometer accuracy, and the underlying dynamic information on virus infection can be extracted to uncover the infection mechanisms of viruses. Therefore, QD-based single-virus tracking (QSVT) is an exquisitely selective and powerful approach to investigating how viruses are internalized in host cells dynamically to release their genome for viral replication and assembly that ensure the completion of viral life cycles.QDs are better candidates than organic dyes and fluorescent proteins for virus labeling and subsequent SVT due to the following considerations: (i) the high brightness of QDs makes it possible to label a virus with sufficient brightness using very few QDs or even just one QD; (ii) the extraordinary photostability of QDs allows one to track the infection process long term and quantify low probability events; (iii) the color-tunable emission property of QDs ensures multicolor labeling of various components of a virus simultaneously; and (iv) the abundant surface ligands of QDs facilitate the conjugation of a virus with a variety of labeling strategies. Therefore, the photoproperties of QDs make it possible to perform multicolor long-term SVT experiments quantitatively. Nowadays, the QD-based SVT (QSVT) technique has made prodigious achievements in unraveling the entry, trafficking, and uncoating mechanisms of viruses. This fascinating technique can provide spatiotemporal dynamic information on the viral journey in unprecedented detail and has revolutionized our understanding of virus infection.In this Account, we first introduce the advantages and the limitations of conventional SVT in virological research and the unique features of QDs as labels in the SVT field. We subsequently focus on the principles and related methods of QSVT and the current state of QD chemistry and QD-based virus labeling that resolves many issues associated with the tracking of individual viruses in live cells. Then we emphasize some new findings by this technique in the study of infection mechanisms. Finally, we will provide our insights into future challenges on this topic. With this Account, we hope to further stimulate the development of QSVT with a combined effort from different disciplines and, more importantly, to accelerate the applications of QSVT in virological research.
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http://dx.doi.org/10.1021/acs.accounts.1c00276DOI Listing
July 2021

Arthroscopic Treatment for Femoroacetabular Impingement Syndrome with External Snapping Hip: A Comparison Study of Matched Case Series.

Orthop Surg 2021 Jun 17. Epub 2021 Jun 17.

Department of Orthopaedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China.

Objective: To determine the effectiveness of hip arthroscopy combined with endoscopic iliotibial band (ITB) release in patients with both femoroacetabular impingement (FAI) syndrome and external snapping hip (ESH).

Methods: Retrospectively review the preoperative and minimum of 2-year follow-up data of patients with both FAI syndrome and ESH who underwent endoscopic ITB release during hip arthroscopy (FAI + ESH group) from January 2014 to December 2018. The same number of age- and gender-matched FAI syndrome patients without ESH undergoing hip arthroscopy were enrolled in the control group (FAI group). Patient-reported outcomes (PROs) including international Hip Outcome Tool (iHOT-33), modified Harris Hip Score (mHHS), visual analog scale for pain (VAS-pain), and abductive force of affected hip at 3 month and 2 years postoperatively were comparatively analyzed. The VAS-satisfaction score of two groups at 2 years postoperatively were also analyzed.

Results: The prevalence of ESH in FAI syndrome patients undergoing hip arthroscopy in our institution was 5.5% (39 of 715 hips), including nine males (10 hips) and 29 females (29 hips). The mean age at the time of surgery was 32.1 ± 6.9 years (range, 22-48 years). According to inclusion and exclusion criteria, 23 patients were enrolled in FAI + ITB group. Twenty-three age- and sex-matched FAI syndrome patients were enrolled in FAI group. At 24 months postoperatively, no patient still suffered ESH symptoms and painful palpation at lateral region in FAI + ITB group. The iHOT-33, mHHS, and VAS-pain score of patients in FAI + ESH group were significantly severer than patients in FAI group preoperatively (41.6 ± 7.5 vs 48.8 ± 7.2, 54.8 ± 7.2 vs 59.2 ± 6.9, 5.5 ± 0.9 vs 4.7 ± 1.0; P < 0.05), while there was no significant difference in these scores between the patients in FAI + ESH group and FAI group at 3-month and 24-month follow-up (73.6 ± 8.5 vs 76.1 ± 6.9, 85.3 ± 7.8 vs 84.2 ± 6.6, 0.8 ± 0.9 vs 0.6 ± 0.9; P > 0.05). At 3 months after surgery, the abductive force of operated hip was significantly smaller than that in FAI group (82.4 ± 12.4 N vs 91.9 ± 16.1 N, P < 0.05), whereas there was no significant difference at 24 months after surgery (101.6 ± 14.9 N vs 106.5 ± 13.7 N, P > 0.05). The VAS-satisfaction scores of patients in the two groups were at a similarly high level (90.5 ± 6.8 vs 88.8 ± 7.3, P > 0.05). There was no complication and no arthroscopic revision in either group until 2-year follow-up.

Conclusion: Although abductive force recovery of the hip was delayed, hip arthroscopy combined with endoscopic ITB release addressed hip snapping in patients with both FAI syndrome and ESH, and could get similar functional improvement, pain relief, recovery speed, as well as patient satisfaction compared with the pure hip arthroscopy in FAI syndrome patients without ESH.
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http://dx.doi.org/10.1111/os.13109DOI Listing
June 2021

Therapeutic effects of the TST36 stapler on rectocele combined with internal rectal prolapse.

World J Gastrointest Surg 2021 May;13(5):443-451

Department of Anorectal Disease, Shenyang Coloproctology Hospital, Shenyang 110000, Liaoning Province, China.

Background: The most common causes of outlet obstructive constipation (OOC) are rectocele and internal rectal prolapse. The surgical methods for OOC are diverse and difficult, and the postoperative complications and recurrence rate are high, which results in both physical and mental pain in patients. With the continuous deepening of the surgeon's concept of minimally invasive surgery and continuous in-depth research on the mechanism of OOC, the treatment concepts and surgical methods are continuously improved.

Aim: To determine the efficacy of the TST36 stapler in the treatment of rectocele combined with internal rectal prolapse.

Methods: From January 2017 to July 2019, 49 female patients with rectocele and internal rectal prolapse who met the inclusion criteria were selected for treatment using the TST36 stapler.

Results: Forty-five patients were cured, 4 patients improved, and the cure rate was 92%. The postoperative obstructed defecation syndrome score, the defecation frequency score, time/straining intensity, and sensation of incomplete evacuation were significantly decreased compared with these parameters before treatment, and the differences were statistically significant ( < 0.05). The postoperative anal canal resting pressure and maximum squeeze pressure in patients decreased compared with before treatment, and the differences were statistically significant ( < 0.05). The initial and maximum defecation thresholds after surgery were significantly lower than those before treatment, and the differences were statistically significant ( < 0.05). The postoperative ratings of rectocele, resting phase, and defecation phase in these patients were significantly decreased compared with those before treatment, and the differences were statistically significant ( < 0.05).

Conclusion: The TST36 stapler is safe and effective in treating rectocele combined with internal rectal prolapse and is worth promoting in clinical work.
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http://dx.doi.org/10.4240/wjgs.v13.i5.443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167842PMC
May 2021

Long non-coding RNA FEZF1-AS1 promotes the proliferation and metastasis of hepatocellular carcinoma via targeting miR-107/Wnt/β-catenin axis.

Aging (Albany NY) 2021 05 23;13(10):13726-13738. Epub 2021 May 23.

Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.

Hepatocellular carcinoma (HCC) is a public health problem around the world, with the molecular mechanisms being still incompletely clear. This study was carried out to explore the role and mechanism of long-noncoding RNA (lncRNA) FEZF1-AS1 in HCC progression. RNA sequencing and quantitative real time polymerase chain reaction (qRT- PCR) were applied to identify differently expressed lncRNAs in HCC tissues and adjacent normal tissues. CCK8 assay was adopted to test cell proliferation and flow cytometry was taken to detect cell apoptosis. Wound healing assay and transwell experiment were performed to determine cell migration and invasion. To validate the function of lncRNA FEZF1-AS1 , tumor-burdened models were established. The results showed that lncRNA FEZF1-AS1 level was prominently enhanced in HCC tumor specimens and overexpression of FEZF1-AS1 promoted the proliferation, migration and invasion of HCC cells. In mechanism, overexpression of FEZF1-AS1 reduced the expression of miR-107 which inhibited the activation of Wnt/β-catenin signaling. Overexpression of β-catenin promoted cell proliferation, migration and invasion which were inhibited by FEZF1-AS1 downregulation. In conclusion, our study demonstrated that FEZF1-AS1 promoted HCC progression through activating Wnt/β-catenin signaling by targeting miR-107, which provided a novel target for the therapy of HCC.
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http://dx.doi.org/10.18632/aging.202960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202841PMC
May 2021

Proximity-induced exponential amplification reaction triggered by proteins and small molecules.

Chem Commun (Camb) 2021 May;57(38):4714-4717

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, and School of Medicine, Nankai University, Tianjin 300071, P. R. China.

We proposed a method to regulate nucleic acid polymerization by proximity and designed an ultrasensitive biosensor based on proximity-induced exponential amplification reaction for proximity assay of proteins (streptavidin) and small molecules (adenosine triphosphate), which allows us to detect a variety of interesting targets by simply changing the binding sites of DNA.
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http://dx.doi.org/10.1039/d1cc00583aDOI Listing
May 2021

Accurate and Efficient Lipoprotein Detection Based on the HCR-DNAzyme Platform.

Anal Chem 2021 04 9;93(15):6128-6134. Epub 2021 Apr 9.

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, and School of Medicine, Nankai University, Tianjin 300071, P. R. China.

Cardiovascular disease is one of the main causes of death in the world, which is closely associated with dyslipidemia. Dyslipidaemia is usually manifested as a relatively higher level of low-density lipoprotein (LDL) and lower level of high-density lipoprotein (HDL). Thus, the quantitative detection of the LDL and HDL particles is of great importance to predict the risk of cardiovascular diseases. However, the traditional methods can only indirectly reflect the HDL/LDL particle concentrations by detecting the cholesterol or proteins in HDL/LDL particles and are always laborious and time-consuming. Thus, the accurate and efficient approach for the detection of intact HDL and LDL particles is still lacking so far. We developed an enzyme- and isolation-free method to measure the concentration of HDL and LDL based on DNAzyme and hybridization chain reaction (HCR)-based signal amplification. This method can be used to directly and accurately detect the concentration of "actual" HDL and LDL particles instead of the cholesterol in HDL and LDL, with limits of detection of 10 and 30 mg/dL, respectively, which also satisfied the lipoprotein analysis in clinical samples. Therefore, this HCR-DNAzyme platform has great potential in clinical applications and health management.
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http://dx.doi.org/10.1021/acs.analchem.0c05322DOI Listing
April 2021

Mn, N, P-tridoped bamboo-like carbon nanotubes decorated with ultrafine CoP/FeCo nanoparticles as bifunctional oxygen electrocatalyst for long-term rechargeable Zn-air battery.

J Colloid Interface Sci 2021 May 23;590:330-340. Epub 2021 Jan 23.

Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Sciences, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua 321004, China. Electronic address:

Rational synthesis of cost-effectiveness, ultra-stable and high-efficiency bifunctional oxygen catalysts are pivotal for Zn-air batteries. Herein, fine CoP/FeCo nanoparticles (NPs) anchored on Mn, N, P-codoped bamboo-like carbon nanotubes (CoP/FeCo/MnNP-BCNTs) are constructed in the coexistence of melamine, poly(4-vinylpyridine) and adenosine-5'-diphosphate disodium salt (ADP) by convenient pyrolysis and follow-up acid treatment. The as-prepared catalyst exhibits the higher onset potential (E = 0.97 V vs. RHE) and half-wave potential (E = 0.88 V vs. RHE) for oxygen reduction reaction (ORR), coupled with excellent oxygen evolution reaction (OER) with the lower overpotential of 324 mV at 10 mA cm. Notably, the home-made Zn-air battery delivers the greater peak power density of 220 mW cm, together with the outstanding cycling stability. The excellent performances of CoP/FeCo/MnNP-BCNTs catalyst are mainly attributed to the highly conductive carbon nanotubes and the synergistic effects between carbon nanotubes and CoP/FeCo NPs. This work offers a novel strategy to explore advanced bifunctional oxygen catalysts for high-efficiency metal-air batteries.
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http://dx.doi.org/10.1016/j.jcis.2021.01.053DOI Listing
May 2021

Real-Time Dissecting the Dynamics of Drug Transportation in the Live Brain.

Nano Lett 2021 01 8;21(1):642-650. Epub 2020 Dec 8.

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, and School of Medicine, Nankai University, Tianjin 300071, China.

Brain diseases are becoming a more and more serious threat to human health. Many critical properties of the transport mechanisms of drugs in live brains remain poorly understood. In this work, single-particle tracking was used to dissect the transport dynamics of wheat germ agglutinin (WGA) in live brain and characterize the geometry and rheology of the extracellular space (ECS). The results revealed that the movements of WGA were influenced by the specific-binding molecules and the nature of the ECS. We further analyzed the mobility behaviors of WGA globally and quantitatively and found that movement of WGA in brain cells of acute slices was an active transport process associated with actin filaments and microtubules. This work paves the way for studies aiming at characterizing the biophysics of drug transport in the context of live brains, which may contribute to developing potential new therapeutic applications for brain diseases.
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http://dx.doi.org/10.1021/acs.nanolett.0c04216DOI Listing
January 2021

A dual-functional fluorescent probe for sequential determination of Cu/S and its applications in biological systems.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Dec 6;243:118797. Epub 2020 Aug 6.

Department of Chemical Biology and Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China. Electronic address:

A new acylhydrazine-derived Schiff base fluorescence probe DMI based on "ON-OFF-ON" fluorescence strategy was presented in this paper. Probe DMI could detect Cu selectively and sensitively with dramatic fluorescence quenching in CHOH-PBS (v/v = 3:7) mixed solution. Once the complex DMI-Cu interacted with S, 10.67-folds fluorescence increase was induced via a displacement mechanism under the same experimental conditions. The corresponding detection limits for Cu and S were calculated to be 1.52 × 10 M and 1.79 × 10 M, respectively. The structures of DMI and DMI-Cu were systematically characterized by Job's plot analysis, ESI-MS, IR, X-ray diffraction and density functional theory calculations. Furthermore, fluorescence imaging in MCF-7 cells and zebrafish demonstrated the probe DMI could act as a useful tool to monitor and track intracellular Cu and S, which was encouraged by remarkable fluorescence performance and low cytotoxicity. Importantly, the complex DMI-Cu could be applied to detect corrupt blood samples, which could estimate the time of death.
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http://dx.doi.org/10.1016/j.saa.2020.118797DOI Listing
December 2020

A novel hydrazide Schiff base self-assembled nanoprobe for selective detection of human serum albumin and its applications in renal disease surveillance.

J Mater Chem B 2020 09;8(36):8346-8355

Department of Chemical Biology and Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, P. R. China.

Human serum albumin (HSA) is considered as a biomarker for the early diagnosis of renal disease, therefore identifying and detecting HSA in biological fluids (especially urine) with an easy method is of great importance. Herein, we report a novel hydrazide Schiff base fluorescent probe N'-((7-(diethylamino)-2-oxo-2H-chromen-3-yl)methylene)pyrazine-2-carbohydrazide (NPC), which self-assembled into nanoparticles in aqueous solution. Based on disassembly-induced emission and the site-specific recognition mechanism, the binding of NPC with HSA resulted in a fluorescence "turn-on" response. Probe NPC exhibited superior selectivity and sensitivity toward HSA with a detection limit of 0.59 mg L-1 in PBS and 0.56 mg L-1 in the urine sample. The site-binding mechanism of NPC with HSA was explored by fluorescence quenching study, Job's plot analysis, HSA destruction, site marker displacement and molecular docking. Fluorescence imaging of HSA in MCF-7 cells was achieved by using a non-toxic NPC probe, suggesting that NPC could be applied to visualize the level of HSA in vivo. More importantly, further practical applications of probe NPC in human urine samples were achieved with satisfactory results by using a fluorometer or test paper, which could provide extensive application in clinical diagnosis.
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http://dx.doi.org/10.1039/d0tb01411gDOI Listing
September 2020

Low-Frequency Vibration Promotes Tumor Necrosis Factor-α Production to Increase Cartilage Degeneration in Knee Osteoarthritis.

Cartilage 2020 Jun 12:1947603520931178. Epub 2020 Jun 12.

West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.

Objective: Low-frequency vibration accelerates cartilage degeneration in knee osteoarthritis (KOA) rat model. In this article, we investigated whether whole-body vibration (WBV) increases cartilage degeneration by regulating tumor necrosis factor-α (TNF-α) in KOA.

Design: Proteomics analysis was used to filter candidate protein from synovial fluid (SF) in KOA people after WBV. Enzyme-linked immunosorbent assay (ELISA) was used to estimate changes in TNF-α levels in SF. The C57 mice and TNF-α knock-out mice were sacrificed for the KOA model and WBV intervention. The cartilage was tested by ELISA, histology, terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL), immunohistochemistry, and reverse transcriptase polymerase chain reaction. Luciferase activity test study was conducted to confirm the relationship between TNF-α and the candidate protein.

Results: Differentially expressed proteins were enriched in the glycolytic process, glucose catabolic, and regulation of interleukin-8 (IL-8) secretion processes. Phosphoglycerate kinase, triosephosphate isomerase 1, T cell immunoglobulin- and mucin-domain-containing molecules 2, fumarylacetoacetate hydrolase (FAH), and TNF were the hub node. TNF-α expression increased in SF after WBV ( < 0.05). The cartilage was more degenerated in the TNF-α mice group compared to controls. A significant change was observed in collagen II and FAH ( < 0.05). TNF-α expression improved in C57 mice ( < 0.05). Apoptosis of chondrocytes was inhibited in TNF-α mice by the TUNEL test. Luciferase activity significantly increased in TNF-α + FAH-Luc cells ( < 0.05).

Conclusion: A novel mechanism underlying WBV-triggered cartilage degeneration was found in KOA that demonstrated the critical regulatory function of TNF-α and FAH during WBV.
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http://dx.doi.org/10.1177/1947603520931178DOI Listing
June 2020

New Approaches in Urban Forestry to Minimize Invasive Species Impacts: The Case of Xiongan New Area in China.

Insects 2020 May 12;11(5). Epub 2020 May 12.

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

China is implementing an extensive urban forestry plan in Xiongan New Area (XNA), a new city in Hebei province. The city has been designated to serve Beijing's noncapital functions and promote the integration of the broader Beijing-Tianjin-Hebei city-region. As part of a green initiative to minimize environmental impacts and its carbon footprint, a massive urban forestry system has been planned on an unprecedented scale, expected to cover over 600 km by 2030. Using science to inform policy, one major goal is to simultaneously minimize impacts of invasive species, while making urban forests more resilient to potential invasive species threats. In this review, we introduce these urban forestry plans such as basic concepts and principles for afforestation, tree species to be planted, delineation of existing pests already established, and expected forest invasive species of concern threatening the new area. Finally, we introduce a framework for invasive pest management strategies in XNA based on a "big data" approach and decision system to minimize impacts of invasive species. This new approach to urban forestry has the potential to become an exemplary global model for urban forestry planning, one that integrates research activities focused on forest health surveys and monitoring with sustainable forestry management. Finally, we provide an overview of the forest health policy required for the design of an unprecedentedly large new urban forest from initial planning to full implementation of an integrated forest management program.
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http://dx.doi.org/10.3390/insects11050300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290593PMC
May 2020

The production of graphene using impinging jet exfoliation in a binary system of CO and N-methyl pyrrolidone.

Nanotechnology 2020 Apr 12;31(26):265601. Epub 2020 Mar 12.

State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, People's Republic of China.

High quality and high quantity few-layer graphene was successfully prepared using a new impinging jet method. Natural graphite flakes were first agitated in N-methyl pyrrolidone (NMP) with the assistance of supercritical CO, then the half-exfoliated graphite was further stripped using the shear stress derived from the impinging jets. After the energy conversion and stress analysis of the graphite particles during the whole exfoliation process, it was revealed that the size of the target mesh, the distance between the nozzle and the target, the decompression rate, and the size of the raw materials had a significant influence on the exfoliation process. Additionally, a microscopic view of the exfoliation and dispersion mechanism of graphene in the CO-NMP system was investigated using molecular dynamics simulation, and CO was found to be beneficial for the penetration of NMP into the graphite sheets. Finally, the concentration and quality characteristics of the prepared graphene were characterized using ultraviolet-visible spectroscopy, transmission electron microscopy, Raman spectroscopy, and atomic force microscopy. The maximum concentration was as high as 0.689 mg ml, the thickness of 68% of the product was less than 2.5 nm, and the lateral dimension was from 0.5 to 3.0 μm. These results indicate that this impinging jet method is promising for large-scale industrial production.
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http://dx.doi.org/10.1088/1361-6528/ab7f7cDOI Listing
April 2020

Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in 'Zaosu' Pear ( Rehd.) Fruit.

Biomolecules 2020 02 11;10(2). Epub 2020 Feb 11.

College of Horticulture, Northwest A&F University, Taicheng Road NO.3, Yangling 712100, Shaanxi Province, China.

Anthocyanin contributes to the coloration of pear fruit and enhances plant defenses. Members of the ethylene response factor (ERF) family play vital roles in hormone and stress signaling and are involved in anthocyanin biosynthesis. Here, was identified from the lanolin-induced red fruit of 'Zaosu' pear ( Rehd.) using a comparative transcriptome analysis. Its expression level was up- and down-regulated by methyl jasmonate and 1-methylcyclopropene plus lanolin treatments, respectively, which indicated that responded to the jasmonate- and ethylene-signaling pathways. In addition, transiently overexpressed induced anthocyanin biosynthesis in 'Zaosu' fruit, and a quantitative PCR analysis further confirmed that facilitated the expression of anthocyanin biosynthetic structural and regulatory genes. Moreover, a dual luciferase assay showed that enhanced the activation effects of and on the promoter. Therefore, responses to jasmonate and ethylene signals and regulates anthocyanin biosynthesis. This provides a new perspective on the correlation between jasmonate-ethylene crosstalk and anthocyanin biosynthesis.
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http://dx.doi.org/10.3390/biom10020278DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072184PMC
February 2020

Single-Virus Tracking: From Imaging Methodologies to Virological Applications.

Chem Rev 2020 02 17;120(3):1936-1979. Epub 2020 Jan 17.

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, and School of Medicine , Nankai University , Tianjin 300071 , P. R. China.

Uncovering the mechanisms of virus infection and assembly is crucial for preventing the spread of viruses and treating viral disease. The technique of single-virus tracking (SVT), also known as single-virus tracing, allows one to follow individual viruses at different parts of their life cycle and thereby provides dynamic insights into fundamental processes of viruses occurring in live cells. SVT is typically based on fluorescence imaging and reveals insights into previously unreported infection mechanisms. In this review article, we provide the readers a broad overview of the SVT technique. We first summarize recent advances in SVT, from the choice of fluorescent labels and labeling strategies to imaging implementation and analytical methodologies. We then describe representative applications in detail to elucidate how SVT serves as a valuable tool in virological research. Finally, we present our perspectives regarding the future possibilities and challenges of SVT.
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http://dx.doi.org/10.1021/acs.chemrev.9b00692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075663PMC
February 2020

A highly selective colorimetric and fluorescent probe for quantitative detection of Cu/Co: The unique ON-OFF-ON fluorimetric detection strategy and applications in living cells/zebrafish.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Mar 6;228:117763. Epub 2019 Nov 6.

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China. Electronic address:

Identifying and detecting similar target cations through combining "turn on" and "turn off" fluorescence mechanism is effective and challenging. Now a new colorimetric and ON-OFF-ON fluorescent probe N'-((7-(diethylamino)-2-oxo-2H-chromen-3-yl)methylene)-3-hydroxy-2-naphthohydrazide (L) was reported, which could detect Cu and Co in phosphate buffered CHCHOH-HO solvent system. With the assistance of glutathione and pH adjustment, a unique ON-OFF-ON fluorescence detection strategy could be achieved for distinguishing Cu and Co. The emission of probe could recover from the L-Cu and L-Co system by addition of GSH or adjusting pH value to 4, respectively, which is due to the abolishment of paramagnetic Cu/Co. Based on fluorescence titration experiments, the limit of detection was determined as 3.84 × 10 M and 4.55 × 10 M for Cu and Co, respectively. Meanwhile, the detection limit reached 6.21 × 10 M for Cu and 6.96 × 10 M for Co according to absorbance signal output. Fast recognition of Cu/Co can be achieved by obvious color changes from green to colorless under UV light, as well as from yellow to orange-red in room light. The binding mode of L toward Cu and Co have been systematically studied by Job's plot analysis, ESI-MS, IR and density functional theory calculations. Most strikingly, further practical applications of the probe L in fluorescence imaging were investigated in MCF-7 cells and zebrafish due to its low cytotoxicity and good optical properties, suggesting that L could serve as a fluorescent sensor for tracking Cu and Coin vivo.
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http://dx.doi.org/10.1016/j.saa.2019.117763DOI Listing
March 2020

Immature dendritic cells derived exosomes promotes immune tolerance by regulating T cell differentiation in renal transplantation.

Aging (Albany NY) 2019 10 26;11(20):8911-8924. Epub 2019 Oct 26.

Department of Kidney Transplantation, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.

Objective: To investigate the mechanism of immature dendritic cells-derived exosomes (imDECs) in the regulation of T cell differentiation and immune tolerance in renal allograft model mice.

Results: imDECs significantly improved the percent of survival, relieved inflammatory response, and reduced CD4+T cell infiltration. In addition, imDECs reduced the rejection associated cytokines in allograft mice, and increased the percentage of Foxp3+CD4+T cells in spleen and kidney tissues. imDECs suppressed the IL17+CD4+T cells and promoted the Foxp3+CD4+T cells under Th17 polarization condition. Moreover, miR-682 was found to be highly expressed in imDECs which suppressed the IL17+CD4+T cells and promoted the Foxp3+CD4+T cells. Luciferase reporter assay showed ROCK2 was a target of miR-682, and ROCK mRNA level was negative correlated with miR-682 mRNA level.

Conclusion: miR-682 was highly expressed in imDECs, and imDECs-secreted miR-682 promoted Treg cell differentiation by negatively regulating ROCK2 to promote immune tolerance in renal allograft model mice.

Methods: Renal allograft model mice were established, and imDECs or mature dendritic cells-derived exosomes (mDECs) were injected into model mice. Rejection associated cytokines IFN-γ, IL-2, IL-17 levels in plasma were detected by ELISA. IL-17A, Foxp3, miR-682, ROCK2, p-STAT3, p-STAT5 expressions were measured by qRT-PCR or western blot.
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http://dx.doi.org/10.18632/aging.102346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834404PMC
October 2019

Effect of oral nutritional supplementation on the post-discharge nutritional status and quality of life of gastrointestinal cancer patients after surgery: a multi-center study.

Asia Pac J Clin Nutr 2019 ;28(3):450-456

Department of General Surgery, Beijing Hospital, National Geriatrics Center, Beijing, China.

Background And Objectives: To evaluate the effect of oral nutritional supplementation (ONS) on the postdischarge nutritional status and quality of life (QoL) of gastrointestinal cancer patients after surgery.

Methods And Study Design: A multi-center study was conducted on gastrointestinal cancer patients who received surgical treatment from 2013-2015. All patients were screened using the Nutrition Risk Screening 2002 (NRS 2002) to assess nutritional risk. Patients with nutritional risk were randomized into two groups: patients in the study group (n=55) were given dietary guidance and ONS, control group (n=59) received only dietary guidance. Anthropometric measurements, nutrition-related laboratory tests, and gastrointestinal function scores were also collected and analyzed using Student's t test and analysis of variance (ANOVA). In addition, the EQ-5D was used to evaluate patients' QoL.

Results: Compared with baseline measurements, the body weight of patients in the study group increased by 1.35±0.53 kg and 1.35±0.73 kg at 60 and 90 days, which were significantly higher than those in the control group (-1.01±0.54 kg, and -1.60±0.81 kg at 60 and 90 days). The results from ANOVA showed that only weight and BMI differed significantly between the study and control groups and also between different measurement times (p<0.01). No differences were found for the other indicators or QoL between the study groups.

Conclusions: ONS may improve the weight and BMI of surgically treated gastrointestinal cancer patients postdischarge. However, these effects had little impact on patients' QoL.
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http://dx.doi.org/10.6133/apjcn.201909_28(3).0004DOI Listing
February 2020

Bortezomib ameliorates acute allograft rejection after renal transplant by inhibiting Tfh cell proliferation and differentiation via miR-15b/IRF4 axis.

Int Immunopharmacol 2019 Oct 1;75:105758. Epub 2019 Aug 1.

Department of Kidney Transplantation, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China. Electronic address:

Objective: The present study aimed to investigate the functional role of bortezomib in the development of acute allograft rejection (AR) after renal transplant.

Methods: The mouse model of AR was established by allograft kidney transplant followed by the treatment of bortezomib. The serum cytokines, renal function, and the percentage of T follicular helper (Tfh) cells in CD4 T cells were measured. The effect of miR-15b and interferon-regulatory factor 4 (IRF4) on Tfh cell proliferation and differentiation was assessed by cell transfection technology and CCK-8 assay. The interaction between miR-15b and IRF4 was assessed by luciferase reporter assay.

Results: Bortezomib relieved acute AR after renal transplant by suppressing Tfh cell proliferation and differentiation. Meanwhile, bortezomib treatment markedly increased miR-15b expression in AR renal tissues. The upregulation of miR-15b inhibited Tfh cell proliferation and differentiation by reducing IRF4. In addition, bortezomib ameliorated AR by suppressing Tfh cell proliferation and differentiation through miR-15b/IRF4 axis in vitro and in vivo.

Conclusion: Our findings indicated the mechanism underlying the bortezomib in treating acute AR after renal transplant, and suggested the critical role of miR-15b in Tfh cell proliferation and differentiation, which provided a therapeutic target in attenuating acute AR.
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http://dx.doi.org/10.1016/j.intimp.2019.105758DOI Listing
October 2019

3D highly branched PtCoRh nanoassemblies: Glycine-assisted solvothermal synthesis and superior catalytic activity for alcohol oxidation.

J Colloid Interface Sci 2019 Oct 13;554:512-519. Epub 2019 Jul 13.

Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Sciences, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua 321004, China. Electronic address:

Advanced Pt-based ternary nanocatalysts display dramatically enhanced utilization efficiency of Pt alternative to mono- and bi-counterparts, owing to the synergistic effects of the tri-metals. Herein, multicomponent uniform 3D PtCoRh highly branched nanoassemblies (HBNAs) were prepared by glycine-assisted one-pot solvothermal method in oleylamine (OAm). The effects of the precursor types, reaction time and amount of glycine were critically investigated in this synthesis. The as-prepared PtCoRh HBNAs displayed outstanding electrocatalytic activity and improved stability towards ethanol oxidation reaction (EOR) and methanol oxidation reaction (MOR) in 1 M KOH electrolyte, whose mass/specific activities were 1.75 A mg/4.03 mA cm and 0.98 A mg/2.34 mA cm, respectively, which were remarkably higher than commercial Pt/C (0.85 A mg/4.03 mA cm and 0.47 A mg/0.89 mA cm). This study provides some novel guidelines to fabricate advanced multimetallic electrocatalysts for practical applications in direct alcohol fuel cells (DAFCs).
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http://dx.doi.org/10.1016/j.jcis.2019.07.030DOI Listing
October 2019

A dual functional turn-on non-toxic chemosensor for highly selective and sensitive visual detection of Mg and Zn: the solvent-controlled recognition effect and bio-imaging application.

Analyst 2019 Jul 29;144(13):4024-4032. Epub 2019 May 29.

Department of Chemical Biology and Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, P. R. China.

A new dual functional turn-on chemosensor, 2,6-diformyl-4-methylphenol-di(isoquinolinyl-1-hydrazone) (HL), has been developed, which could highly selectively discriminate Mg and Zn in different solvent systems. The chemosensor HL exhibits rapid visual turn-on fluorescence enhancing recognition toward Mg/Zn, which is not interfered by other cations, especially for respective congeners Ca/Cd. The remarkable fluorescence enhancement (71-fold or 11-fold) was observed after adding Mg in acetonitrile or Zn in DMF-HO solvent systems. Additionally such a solvent medium-controlled platform could achieve the quantitative determination of Mg and Zn quantitation with low detection limits of 2.97 × 10 M and 3.07 × 10 M, respectively. Furthermore, the turn-on fluorescence sensing mechanism is also investigated by H NMR, FT-IR and ESI-MS spectroscopy. Density functional theory (DFT) calculations derive optimized geometries of HL and its complexes. Notably, non-toxic HL also can be successfully applied as a visual probe for the practical determination of Mg/Zn in MCF-7 cells, Zebrafish larvae, syrup and water samples, which might provide extensive application in biology and medicine fields.
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http://dx.doi.org/10.1039/c9an00583hDOI Listing
July 2019

Thiel-embalming technique: investigation of possible modification in embalming tissue as evaluation model for radiofrequency ablation.

J Biomed Res 2019 Apr 8. Epub 2019 Apr 8.

Institute for Medical Science and Technology, University of Dundee, Dundee, Scotland DD2 1FD, United Kingdom.

Contrary to freezing preservation and formalin embalming, Thiel embalmed cadaver presents soft texture and color very close to that of living organism, and many applications based on Thiel embalmed cadavers have been reported. However, Thiel embalmed cadavers cannot be used as reliable evaluation model for radiofrequency ablation (RFA) due to dramatic changes of electrical conductivity in the embalmed tissue. To address this issue, we investigated various modifications of the original Thiel embalming solution. By altering the chemicals' species and concentration we figured out a formula that can greatly reduce the embalming fluid's electrical conductivity without significantly compromising the 18-day embalmed kidney samples' suppleness and color. We also investigated a two-stage embalming technique by first submerging the kidney sample into original Thiel's tank fluid for 28 days, then the sample was withdrawn from the tank fluid and placed into modified dilution fluids for additional two weeks. Stiffening and discoloration occurred in these diluted samples implying the reversibility of Thiel-embalmed tissues' suppleness and color with the removal of the strong electrolytes. This study presents a modified embalming method which could be used for RFA evaluation and also helps our understanding of the mechanism of embalmment process.
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http://dx.doi.org/10.7555/JBR.32.20160148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813523PMC
April 2019

Low-Intensity Focused Ultrasound-Responsive Phase-Transitional Nanoparticles for Thrombolysis without Vascular Damage: A Synergistic Nonpharmaceutical Strategy.

ACS Nano 2019 03 14;13(3):3387-3403. Epub 2019 Mar 14.

Department of Radiology , Second Affiliated Hospital of Chongqing Medical University , No. 74 Linjiang Road , Yuzhong District, Chongqing , 400010 , People's Republic of China.

Multimodal molecular imaging has shown promise as a complementary approach to thrombus detection. However, the simultaneous noninvasive detection and lysis of thrombi for cardiovascular diseases remain challenging. Herein, a perfluorohexane (PFH)-based biocompatible nanostructure was fabricated, namely, as-prepared FeO-poly(lactic- co-glycolic acid)-PFH-CREKA nanoparticles (NPs), which combine phase transition (PT) thrombolysis capabilities with properties conducive to multimodal imaging. This well-developed PT agent responded effectively to low-intensity focused ultrasound (LIFU) by triggering the vaporization of liquid PFH to achieve thrombolysis. The presence of the CREKA peptide, which binds to the fibrin of the thrombus, allows targeted imaging and efficacious thrombolysis. Then, we found that, compared with thrombolysis using a non-phase-transition agent, PT thrombolysis can produce a robust decrease in the thrombus burden regardless of the acoustic power density of LIFU. In particular, the reduced energy for LIFU-responsive PT during the lysis process guarantees the superior safety of PT thrombolysis. After injecting the NPs intravenously, we demonstrated that this lysis process can be monitored with ultrasound and photoacoustic imaging in vivo to evaluate its efficacy. Therefore, this nonpharmaceutical strategy departs from routine methods and reveals the potential use of PT thrombolysis as an effective and noninvasive alternative to current thrombolytic therapy.
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http://dx.doi.org/10.1021/acsnano.8b09277DOI Listing
March 2019

Syntheses, characterization, DNA/HSA binding ability and antitumor activities of a family of isostructural binuclear lanthanide complexes containing hydrazine Schiff base.

J Biomol Struct Dyn 2020 02 26;38(3):733-743. Epub 2019 Mar 26.

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, P. R. China.

Three dinuclear lanthanide complexes, [Ln(L)(μ-OAc)(HO)]⋅2HO (Ln = La (), Eu () and Dy (), HL = N'-(2-hydroxybenzylidene) nicotinohydrazide), have been synthesized and characterized by IR, elemental analysis and X-ray single-crystal diffraction. Crystallographic study revealed that the representative complex displays a discrete dinuclear structure with a distorted tricapped trigonal prismatic geometry around La(III) ion. The interaction of complexes - with CT-DNA was investigated by absorption spectra, fluorescence quenching and viscosity, which reveals that the complexes bind to CT-DNA with a moderate intercalative mode. The complexes exhibited obvious DNA cleavage activities in the presence of HO. All complexes could bind to human serum albumin (HSA) with medium affinity through static mode; thus, HSA could effectively transport complexes. Furthermore, three complexes exhibited specific cytotoxicity to A549 cancer cells in micromole magnitude than other cancer cells tested and less toxicity than cisplatin for normal human cells HUVEC, in which massive cell apoptosis was induced by complexes through producing DNA damage and suppressing DNA synthesis.Communicated by Ramaswamy H. Sarma.
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http://dx.doi.org/10.1080/07391102.2019.1587511DOI Listing
February 2020

Uric acid supported one-pot solvothermal fabrication of rhombic-like PtCu hollow nanocages for highly efficient and stable electrocatalysis.

J Colloid Interface Sci 2019 Mar 7;540:486-494. Epub 2019 Jan 7.

Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Sciences, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua 321004, China. Electronic address:

High activity and good durability of electrocatalysts are of significance in practical applications of fuel cells. Among them, multi-component metallic hollow nanocages/nanoframes show great potential as advanced catalysts because of their highly open structures, large surface area and good stability. Herein, we report a general uric acid-mediated solvothermal method for shape-controlled synthesis of rhombic-like PtCu hollow nanocages (HNCs) with uric acid as co-reductant and co-structure-directing agent. Uric acid and cetyltrimethylammonium chloride (CTAC) played important roles in the hollow cages. The specific architectures showed remarkably enhanced catalytic properties towards glycerol oxidation reaction (GOR), ethylene glycol oxidation reaction (EGOR) and oxygen reduction reaction (ORR) with the enhanced specific activity, outperforming commercial Pt/C (20 wt%). This work provides a new avenue for rational design of novel bimetallic nanocatalysts with enhanced characters in energy storage and conversion.
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http://dx.doi.org/10.1016/j.jcis.2019.01.020DOI Listing
March 2019

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

Aging (Albany NY) 2018 12;10(12):3774-3793

Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Increasing evidence has indicated the prognostic value of miR-433 across a series of malignancy types. However, the underlying mechanisms involved in cancer progression haven't been sufficiently elucidated. In the present work, we found that miR-433 was downregulated in CRC tissues and cell lines. Ectopic expression of miR-433 obviously suppressed the proliferation, invasion and metastasis activity of CRC cells in vitro and in vivo. CREB1, CCAR1 and JNK1 were highly expressed and negatively correlated with miR-433 expression in CRC. CRC patients with higher expression of CREB1, CCAR1 or JNK1 presented a worse outcome relative to those with lower expression. CREB1 transactivated the expression of miR-433, and CREB1, CCAR1 and JNK1 simultaneously served as its targets, which in turn composed a feedback loop between CREB1 and miR-433. miR-433 blocked cell cycle progression and abolished EMT. Collectively, our study demonstrated the CREB1/miR-433 reciprocal feedback loop restrained the propagation, invasion and metastasis activities of CRC cells through abrogation of cell cycle progression and constraint of EMT.
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http://dx.doi.org/10.18632/aging.101671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326693PMC
December 2018

Enhanced Photoacoustic and Photothermal Effect of Functionalized Polypyrrole Nanoparticles for Near-Infrared Theranostic Treatment of Tumor.

Biomacromolecules 2019 01 10;20(1):401-411. Epub 2018 Dec 10.

Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, Faculty of Materials and Energy , Southwest University , Chongqing 400715 , China.

Functionalized nanomaterials with near-infrared (NIR) responsive capacity are quite promising for theranostic treatment of tumors, but formation of NIR responsive nanomaterials with enhanced theranostic ability and excellent biocompatibility is still very challenging. Herein, PEGylated indocyanine green (ICG)-loaded polypyrrole nanoparticles (PPI NPs) were designed and successfully formed through selecting polydopamine as the linkage between each component, demonstrating enhanced NIR responsive theranostic ability against tumor. By combining in vitro cell study with in vivo assay, the formed PPI NPs were proven to be fantastically biocompatible while effectively internalization in HeLa cells and retention in HeLa tumor were demonstrated by in vitro flow cytometry/confocal measurement and in vivo photoacoustic imaging assay. With the guidance of photoacoustic imaging, successful photothermal ablation of tumor was achieved by treatment with PPI NPs plus laser, which was much more effective than the group treated with NPs free of ICG. The combined enhanced photoacoustic and photothermal effect is mainly ascribed to the functionalized polypyrrole nanoparticles, which could accumulate in the tumor site more effectively with a relatively longer retention time taking advantage of the nanomaterial-induced endothelial leakiness phenomenon. All these results demonstrating that this designed PPI NPs possessing enhanced NIR responsive property hold great promise for tumor NIR theranostic applications.
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http://dx.doi.org/10.1021/acs.biomac.8b01453DOI Listing
January 2019

Influence of visual cues on oviposition site searching and learning behavior in the parasitic beetle Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae).

Sci Rep 2018 11 26;8(1):17331. Epub 2018 Nov 26.

Key Laboratories for Germplasm Resources of Forest Trees and Forest Protection of Hebei Province, College of Forestry, Agricultural University of Hebei, Baoding, Hebei, China.

Color cues play a key role in the location of hosts and host habitats; learning behavior can allow parasitoids to explore different hosts and reduce environmental uncertainty. However, it remains unclear whether the parasitic beetle Dastarcus helophoroides (Fairmaire) uses and learns visual cues to locate oviposition sites. In this study, we investigated the ability of females to respond to colors and associate the presence of a simulated oviposition site-wood with a trough-with colored substrates after training. Two sets of experiments were conducted: (i) investigating the innate preference for substrate coloration and (ii) investigating the ability to learn to associate substrate color with the presence of simulated oviposition sites, with the beetles being trained to respond to different substrate colors with simulated oviposition sites in sessions on 10 consecutive days. The parasitic beetles displayed an innate preference for the black substrate, but this preference changed after the beetles were trained on substrates of different colors. In the associative learning tests, these beetles laid more eggs on the reward-conditioned substrates than on the black substrate after being trained. Our results suggest that visual cues are learned and used by D. helophoroides during their search for and selection of oviposition sites.
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http://dx.doi.org/10.1038/s41598-018-35580-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255905PMC
November 2018
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