Publications by authors named "Zhi-Fen Zhang"

17 Publications

  • Page 1 of 1

MicroRNA‑202 inhibits endometrial stromal cell migration and invasion by suppressing the K‑Ras/Raf1/MEK/ERK signaling pathway.

Int J Mol Med 2020 Dec 9;46(6):2078-2088. Epub 2020 Oct 9.

Department of Gynecology, The Affiliated Hangzhou Hospital of Nanjing Medical University, Hangzhou, Zhejiang 310008, P.R. China.

The enhanced migratory ability of endometrial stromal cells (ESCs) is a key factor in the formation of functional endometrium‑like tissues outside the uterine cavity during endometriosis (EMS). Although accumulating evidence has suggested the importance of microRNAs (miRNAs) in the pathogenesis of EMS, the role of particular miRNAs in the invasiveness of ESCs remain poorly understood. In the present study, the function of miRNAs in the invasiveness of ESCs, along with the associated underlying mechanism involved, were investigated. Initially, the expression patterns of miRNAs in the ectopic and eutopic endometrium isolated from patients with EMS were analyzed using microarray. MicroRNA‑202‑5p (miR‑202) was selected for further study due to its previously reported suppressive effects on the invasion in various types of cancers. The expression of miR‑202 and K‑Ras in eutopic and ectopic endometrioma tissues were detected using reverse transcription‑quantitative PCR, immunohistochemistry and western blotting. The migration and invasion ability of ESCs was determined using wound healing and Transwell invasion assays, respectively. Compared with that from healthy individuals, miR‑202 expression was demonstrated to be lower in the eutopic endometrium from patients with EMS, which was even lower in ectopic endometrium. Functional experiments in primary ESCs revealed that enhanced miR‑202 expression suppressed the cell invasion and migration abilities, which was also accompanied with increased E‑cadherin and reduced N‑cadherin expression in ESCs, suggesting its potentially suppressive role in epithelial‑mesenchymal transition. K‑Ras is a well‑known regulator of the ERK signaling pathway that was shown to be directly targeted and negatively regulated by miR‑202. In addition, K‑Ras expression was found to be upregulated in the ectopic endometrium, where it correlated negatively with that of miR‑202. Knocking down K‑Ras expression mimicked the anti‑invasive effects of miR‑202 overexpression on ESCs, whilst K‑Ras overexpression attenuated the inhibitory role of miR‑202 overexpression in ESC invasion. The K‑Ras/Raf1/MEK/ERK signaling pathway was also blocked by miR‑202 overexpression. These findings suggested that miR‑202 inhibited ESC migration and invasion by inhibiting the K‑Ras/Raf1/MEK/ERK signaling pathway, rendering miR‑202 a candidate for being a therapeutic target for EMS.
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http://dx.doi.org/10.3892/ijmm.2020.4749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595674PMC
December 2020

Type II caesarean scar pregnancy management by ultrasound-guided local lauromacrogol injection in combination with suction curettage: A case report.

Medicine (Baltimore) 2020 Apr;99(17):e19743

Center of Reproductive Medicine.

Introduction: The implantation of a gestational sac within the scar of a previous caesarean delivery is defined as caesarean scar pregnancy (CSP), which is classified into two types: CSP I and CSP II. CSP II is life threatening, and no clear consensus for CSP II management exists.

Patient Concerns: A 31-year-old woman, gravida 1, para 1, with a previous caesarean delivery due to macrosomia, presented with an estimated 45 days of amenorrhea. The patient presented to the emergency department with vaginal bleeding for 1 day and no abdominal pain.

Diagnoses: An ultrasound examination was performed demonstrating a viable fetus that was embedded in the caesarean scar area and was bulging through the wall of the uterus into the bladder without contact with the uterine cavity or cervical canal. A diagnosis of type II caesarean scar pregnancy was made.

Interventions: Local lauromacrogol was used to reduce the gestational sac blood supply. Suction curettage was performed under the guidance of abdominal ultrasound 24 h later, and the amount of bleeding was 20 mL. The response to the treatment was monitored by serial beta-human chorionic gonadotropin (β-hCG).

Outcomes: Patient was followed up with β-hCG weekly levels which became <10 mIU/mL after 4 weeks of treatment.

Conclusion: Ultrasound-guided local lauromacrogol injection combined with suction curettage may be a safer and novel therapeutic method.
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http://dx.doi.org/10.1097/MD.0000000000019743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220564PMC
April 2020

Downregulation of lncRNA ZFAS1 and upregulation of microRNA-129 repress endocrine disturbance, increase proliferation and inhibit apoptosis of ovarian granulosa cells in polycystic ovarian syndrome by downregulating HMGB1.

Genomics 2020 09 19;112(5):3597-3608. Epub 2020 Apr 19.

Department of Gynecology and Obstetrics, Affiliated Hangzhou People's Hospital of Nanjing Medical University, Hangzhou 310010, Zhejiang, PR China. Electronic address:

Objective: The objective was to find the role of long-non-coding RNA zinc finger antisense 1 (lncRNA ZFAS1)/microRNA (miR)-129/high-mobility group box protein 1 (HMGB1) axis in polycystic ovary syndrome (PCOS).

Methods: Ovarian granulosa cells from non-PCOS patients and PCOS patients were collected, and HMGB1, miR-129 and lncRNA ZFAS1 expression were detected. Ovarian granulosa cells were transfected with si-ZFAS1 or miR-129 mimics to verify their roles in P4 and E2 secretion, and the biological functions of ovarian granulosa cells.

Results: LncRNA ZFAS1 and HMGB1 were elevated, while miR-129 was down-regulated in ovarian granulosa cells of PCOS patients. Down-regulated lncRNA ZFAS1 or overexpressed miR-129 could decrease HMGB1 expression, increase P4 and E2 secretion, promote proliferation activity while inhibit apoptosis of ovarian granulosa cells in PCOS.

Conclusion: LncRNA ZFAS1 could bind to miR-129 to promote HMGB1 expression, thereby affecting the endocrine disturbance, proliferation and apoptosis of ovarian granulosa cells in PCOS.
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http://dx.doi.org/10.1016/j.ygeno.2020.04.011DOI Listing
September 2020

Effects of dipeptidyl peptidase-4 inhibitors on transforming growth factor-β1 signal transduction pathways in the ovarian fibrosis of polycystic ovary syndrome rats.

J Obstet Gynaecol Res 2019 Mar 4;45(3):600-608. Epub 2018 Dec 4.

Department of Gynecology, The Affiliated Hangzhou Hospital of Nanjing Medical University, Hangzhou, Zhejiang, China.

Aim: Examine the effects of dipeptidyl peptidase-4 (DPP4) inhibitor Sitagliptin on the transforming growth factor-β1 (TGF-β1) signal transduction pathway in polycystic ovary syndrome (PCOS) rats with ovarian fibrosis.

Methods: Thirty rats were divided randomly into the PCOS model group, Sitagliptin treatment group and blank control group. Dehydroepiandrosterone was administered to the model group and treatment group to establish the models. Then, the phenotype of rats was recorded, and the serum sex hormone levels were measured. The pathological structures of the rat ovaries were observed. The protein and mRNA expression levels of DPP4, connective tissue growth factor (CTGF), TGF-β1 and Smad2/3 in the ovaries were analyzed.

Results: There was no statistically difference in fasting body weight and blood glucose among the three groups before Sitagliptin treatment (P > 0.05). The fasting blood glucose level was significantly decreased after the administration of Sitagliptin (P < 0.05). The level of testosterone in the model group was reduced remarkably after Sitagliptin treatment (P < 0.001). The protein expression levels of DPP4, CTGF and TGF-β1 in the ovarian stroma were lower in the treatment group than in the model group (P < 0.01, P < 0.001, P < 0.05). The mRNA levels of DPP4, CTGF and TGF-β1 in the model group also greatly declined after Sitagliptin treatment (P < 0.05, P < 0.001, P < 0.01).

Conclusion: The DPP4 inhibitor Sitagliptin lowers fasting blood glucose, relieves the high androgen state of PCOS rats and delays the process of ovarian fibrosis, which may be related to reducing the levels of factors related to the TGF-β1/Smad2/3 signaling pathway.
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http://dx.doi.org/10.1111/jog.13847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587993PMC
March 2019

Puerarin Prevents LPS-Induced Osteoclast Formation and Bone Loss via Inhibition of Akt Activation.

Biol Pharm Bull 2016 ;39(12):2028-2035

College of Medicine, Shaoxing University.

Osteolysis induced by chronic Gram-negative bacterial infection underlies many bone diseases such as osteomyelitis, septic arthritis, and periodontitis. Drugs that inhibit lipopolysaccharide (LPS)-induced osteolysis are critically needed for the prevention of bone destruction in infective bone diseases. In this study, we assessed the effect of puerarin, a natural isoflavone isolated from Pueraria lobata OHWI root, on LPS-induced osteoclastogenesis and bone loss. Our in vitro study showed that puerarin significantly inhibited LPS-induced osteoclast differentiation from osteoclast precursor RAW264.7 cells. The inhibition occurred through suppressing the production of osteoclast activating factor tumor necrosis factor (TNF)-α, interleukin (IL)-1β and prostaglandin E (PGE), which led to down-regulating mRNA expression of osteoclastogenic genes including tartrate-resistant acid phosphatase (TRAP), cathepsin K and matrix metalloprotein 9 (MMP-9). Furthermore, LPS triggered activation of Akt in osteoclast precursor RAW264.7 cells, which was inhibited by puerarin treatment. In vivo, puerarin attenuated LPS-induced bone loss in a murine calvarial osteolysis model. Collectively, puerarin prevents LPS-induced osteoclast formation, function and bone loss, where the inhibition of Akt activation plays an important role. These findings provide evidences that puerarin might be beneficial as a promising candidate drug for the prevention and treatment of bacteria-induced bone destruction disease, and give new insights for understanding its possible mechanism.
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http://dx.doi.org/10.1248/bpb.b16-00522DOI Listing
January 2017

Response to 'Letter to "Uterine perforation in an adolescent using an intrauterine device with memory function: A case report and review of the published work"'.

J Obstet Gynaecol Res 2016 Feb 10;42(2):230. Epub 2015 Nov 10.

Center of Reproductive Medicine, Hangzhou Obstetrics, Gynecology Hospital, Hangzhou, China.

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http://dx.doi.org/10.1111/jog.12861DOI Listing
February 2016

Increase in myeloid-derived suppressor cells (MDSCs) associated with minimal residual disease (MRD) detection in adult acute myeloid leukemia.

Int J Hematol 2015 Nov 10;102(5):579-86. Epub 2015 Sep 10.

Department of Center Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, 250021, People's Republic of China.

Myeloid-derived suppressor cells (MDSCs) are thought to help provide a cellular microenvironments in many solid tumors, in which transformed cells proliferate, acquire new mutations, and evade host immunosurveillance. In the present study, we found that MDSCs (CD33 + CD11b + HLA-DR(low/neg)) in bone marrow were significantly increased in adult acute myeloid leukemia (AML) patients. MDSCs levels in newly diagnosed AML patients correlated well with extramedullary infiltration and plasma D-dimer levels. Remission rates in the MDSCs > 1500 group and MDSCs < 1500 group were 72.73 and 81.25 %, respectively. No significant differences were found between the two groups. MDSC levels in the complete remission group were significantly decreased after chemotherapy, while in the partial remission and non-remission groups, there were no significant differences. The level of MDSCs in the high minimal residual disease (MRD) group was significantly higher than that in the middle and low MRD groups. High levels of Wilms' Tumor-1 (WT-1) protein were strongly correlated with higher bone marrow MDSC levels. In conclusion, we report here a population of immunosuppressive monocytes in the bone marrow of patients with AML characterized by the CD33(high)CD11b + HLA-DR(low/neg) phenotype. These cells appear to impact the clinical course and prognosis of AML. This data may provide potentially important targets for novel therapies.
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http://dx.doi.org/10.1007/s12185-015-1865-2DOI Listing
November 2015

Arbuscular mycorrhizal fungi increase salt tolerance of apple seedlings.

Bot Stud 2014 Dec 9;55(1):70. Epub 2014 Oct 9.

College of Science and Technology, Yantai Institute, China Agricultural University, Binhai Middle Road 2006, Yantai, 264670, China.

Background: Apple trees are often subject to severe salt stress in China as well as in the world that results in significant loss of apple production. Therefore this study was carried out to evaluate the response of apple seedlings inoculated with abuscular mycorrhizal fungi under 0, 2‰, 4‰ and 6‰ salinity stress levels and further to conclude the upper threshold of mycorrhizal salinity tolerance.

Results: The results shows that abuscular mycorrhizal fungi significantly increased the root length colonization of mycorrhizal apple plants with exposure time period to 0, 2‰ and 4‰ salinity levels as compared to non-mycorrhizal plants, however, percent root colonization reduced as saline stress increased. Salinity levels were found to negatively correlate with leaf relative turgidity, osmotic potential irrespective of non-mycorrhizal and mycorrhizal apple plants, but the decreased mycorrhizal leaf turgidity maintained relative normal values at 2‰ and 4‰ salt concentrations. Under salt stress condition, Cl and Na concentrations clearly increased and K contents obviously decreased in non-mycorrhizal roots in comparison to mycorrhizal plants, this caused mycorrhizal plants had a relatively higher K/Na ratio in root. In contrast to zero salinity level, although ascorbate peroxidase and catalase activities in non-inoculated and inoculated leaf improved under all saline levels, the extent of which these enzymes increased was greater in mycorrhizal than in non-mycorrhizal plants. The numbers of survived tree with non-mycorrhization were 40, 20 and 0 (i.e., 66.7%, 33.3% and 0) on the days of 30, 60 and 90 under 4‰ salinity, similarly in mycorrhization under 6‰ salinity 40, 30 and 0 (i.e., 66.7%, 50% and 0) respectively.

Conclusion: These results suggest that 2‰ and 4‰ salt concentrations may be the upper thresholds of salinity tolerance in non-mycorrhizal and mycorrhizal apple plants, respectively.
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http://dx.doi.org/10.1186/s40529-014-0070-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430355PMC
December 2014

Uterine perforation in an adolescent using an intrauterine device with memory function: a case report and review of the published work.

J Obstet Gynaecol Res 2015 Apr 27;41(4):646-9. Epub 2014 Oct 27.

Department of Obstetrics and Gynecology, Hangzhou First People's Hospital of Nanjing Medical University, Hangzhou, China.

Intrauterine devices (IUD) are the most common method of reversible birth control used worldwide. Adolescents infrequently have uterine perforation caused by IUD, hampering both the diagnosis and treatment. Herein, we report a case of uterine perforation in an 18-year-old primipara after insertion of an IUD that possessed memory function (AiMu MCu). The patient presented with vomiting and abdominal pain after insertion of an AiMu MCu IUD, was treated via laparoscopy and had a full recovery. The present case indicated that uterine perforation caused by an AiMu MCu IUD may occur as a rare complication and that uterine perforation can occur in an adolescent. Clinicians might consider a diagnosis of uterine perforation in an adolescent reporting vomiting and abdominal pain after IUD insertion. Laparoscopy could be a reasonable treatment option for such patients.
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http://dx.doi.org/10.1111/jog.12591DOI Listing
April 2015

Indefinite Fetal Heart Rate Pattern in a Patient with Vasa Previa: A Situation Where Guideline Is Inapplicable.

Iran J Public Health 2014 Jan;43(1):120-2

Dept. of Obstetrics and Gynecology, the First People's Hospital of Hangzhou, Nanjing Medical University , Hangzhou, Zhejiang, China.

Most fetal heart rate patterns can be interpreted accurately so that management decisions can be made correctly. How-ever, few fetal heart rate patterns are so ambiguous that the obstetricians cannot interpret them precisely. A 27-year-old woman at 38 weeks' gestation in her first pregnancy was admitted with heavy vaginal bleeding and decrease in fetal movements. Fetal status was indeterminate according to an indefinite fetal heart rate tracing with regular decelerations. After emergent cesarean delivery, a ruptured vasa previa, traversing the fetal membrane, unsupported by either the umbilical cord or placental tissue, was clearly identified. Treatment decision-making is challenging in such patient with indefinite fetal heart rate pattern because limited data exist to guide management. Well-designed studies are needed to clarify the uncertainty about the effect of indefinite fetal heart rate pattern on clinical outcomes.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454033PMC
January 2014

[Protective effects of da chai hu granules (DCHKL) against alloxan (AXN)-induced rat pancreatic islets damage].

Yao Xue Xue Bao 2013 Sep;48(9):1403-8

Pharmacology College, Nanjing University of Chinese Medicine, Nanjing 210046, China.

The protective effects of Da Chai Hu Granules (DCHKL) on islet cells which were incubated with 4 mmol x L(-1) alloxan (AXN) were studied. The viability of islet cells were measured with MTT. Insulin released into medium and in islets was detected by radioimmunoassay. Cell apoptosis rate was determined by flow cytometry. The expression of anti-apoptotic gene Bcl-2 and pro-apoptotic gene Bax in islet cells were measured with RT-PCR (reverse transcription polymerase chain reaction). Serum containing DCHKL can promote the activity of islet cells significantly (P < 0.01). Basal insulin secretion and high glucose-stimulated insulin secretion increased significantly (P < 0.01). Serum containing DCHKL can inhibit apoptosis of islet cells, the ratio of apoptosis was decreased. Serum containing DCHKL increased expression of Bcl-2 mRNA and decreased expression of Bax mRNA. DCHKL can significantly promote proliferation of islet cells and increase the amount of basal secretion of pancreatic islet cells and high glucose-stimulated insulin secretion. The expression of Bcl-2 increased significantly. The expression of Bax decreased significantly. DCHKL have a protective effect on the islet cells.
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September 2013

[Protection and mechanism of Fagopyrum cymosum on lung injury in rats with Klebsiella pneumonia].

Zhong Yao Cai 2012 Apr;35(4):603-7

Department of Pharmacology, Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of Education, Anhui Medical University, Hefei 230032, China.

Objective: To study the mechanism of protective effect of Fagopyrum cymosum on lung injury induced by Klebsiella pneumonia in rats.

Methods: The model of rats with Klebsiella pneumonia was established. The male SD rats were randomly divided into control group, model group, Fagopyrum cymosum (6, 3, 1.5 g/kg) three groups, levofloxacin (25 mg/kg) group. The pathological change of lung was observed. The content of IL-1beta, IL-6, IL-8, TNF-alpha, ICAM-1, INF-gamma in serum were measured by radioimmunoassay and Elisa. TNF-alpha, ICAM-1, NF-kappaB p65 protein expressions were measured by immunohistochemistry. MIP-2mRNA expression was detected by in situ hybridization.

Results: The rats of model group had obvious lung injury, but those of Fagopyrum cymosum and levofloxacin groups had less injury. The contents of IL-1beta, IL-6, IL-,8, TNF-alpha, ICAM-1 and INF-gamma in serum and the expressions of TNF-a, ICAM-1, NF-kappaB p65 and MIP--2mRNA of model group were significantly higher than those of the control group (P < 0.05 or P < 0.01), while the indexes of Fagopyrum cymosum and levofloxacin groups were significantly lower than those of model group (P < 0.05 or P < 0.01).

Conclusion: The lung injury induced by Klebsiella pneumonia is related to TNF-alpha, ICAM-1, NF-kappaB p65 and MIP-2mRNA. To decrease the excessive expression of TNF-alpha, ICAM-1, NF-kappaB p65 and MIP-2mRNA might be the main mechanism of protective effect of Fagopyrum cymosum on lung injury.
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April 2012

Elevated plasma level of HMGB1 is associated with disease activity and combined alterations with IFN-α and TNF-α in systemic lupus erythematosus.

Rheumatol Int 2012 Feb 1;32(2):395-402. Epub 2010 Dec 1.

Department of Central Laboratory, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan 250021, People's Republic of China.

Recent studies indicate that high-mobility group box protein 1 (HMGB1) contributes to the pathogenesis of diverse autoimmune disorders. It induces the production of interferon-alpha (IFN-alpha) and tumor necrosis factor alpha (TNF-alpha) in vitro. In the present study, plasma HMGB1, TNF-alpha, and IFN-alpha were determined with ELISA in 37 patients with systemic lupus erythematosus (SLE) and 39 age- and sex-matched healthy controls (HC). The possible associations of these cytokines with disease activities, autoantibodies, and certain laboratory parameters were also explored. The plasma levels of HMGB1, TNF-alpha, and IFN-alpha were increased in SLE patients compared with those of HC (P < 0.05). Moreover, the levels of HMGB1 and TNF-alpha in the active SLE patients were elevated compared with those in inactive patients and HC. Additionally, plasma HMGB1 was positively correlated with peripheral neutrophils, and plasma TNF-alpha was positively correlated with anti-Sm, ESR and CRP, while plasma IFN-alpha was inversely correlated with the age and platelet level in SLE patients. Our data indicated that increased plasma HMGB1 was associated with disease activity in SLE, which was similar to TNF-alpha. High level of plasma IFN-alpha may be related to nephritis and thrombocytopenia in SLE.
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http://dx.doi.org/10.1007/s00296-010-1636-6DOI Listing
February 2012

[Clinical effect and safety of miconazole nitrate 1200 mg in treating vulvovaginal candidiasis].

Zhonghua Fu Chan Ke Za Zhi 2008 Oct;43(10):757-9

Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University, Shanghai 200001, China.

Objective: To investigate the clinical effect and safety of miconazole nitrate 1200 mg in treating vulvovaginal candidiasis (VVC).

Methods: An open, multicentre, non case control clinical trial was conducted in 568 patients suffering from VVC from Jul 1, 2006 to Nov 30, 2006. Routine examination, score of clinical symptoms and physical signs, mycetology test and safety evaluation were done in all patients before treatment, 7 - 14 days after treatment and 30 days after treatment.

Results: Seven to fourteen days after treatment, 563 patients could be followed and 323 patients (57.3%) were cured. The overall effective rate was 90.2%. The mycologic cure rate was 91.3% (514). Thirty days after treatment, 480 patients could be followed and 411 patients (85.6%) were cured. The total effective rate was 96.0%. Mycologic cure rate was 92.3% (443/480). Adverse effect rate was 2.7% (15/563) and they were relieved without any treatment in one or two days.

Conclusions: Miconazole nitrate 1200 mg is effective in the treatment of VVC, with good compliance and few adverse effects. Moreover, it can be accepted easily.
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October 2008

[Clinical characteristics and treatment of acute asthma exacerbations during pregnancy].

Zhonghua Fu Chan Ke Za Zhi 2008 Jan;43(1):29-31

Department of Obstetrics, Affiliated Obstetric and Gynecology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China.

Objective: To study the clinical characteristics of acute asthma during pregnancy, focusing on management of pregnant women presenting with an acute exacerbation.

Methods: A prospective observational study was conducted in 30 cases of acute exacerbations during pregnancy.

Results: The prognosis of 25 patients with mild or moderate asthma was good for both mother and fetus; none had severe complications. Among 5 women who had severe exacerbations, there were 4 pulmonary co-infections, 1 with respiratory acidosis, 1 with respiratory acidosis plus metabolic alkalosis, and 4 with fetal growth-restriction.

Conclusions: This study highlights that pregnancy can worsen asthma control in some women. Effective management and prevention of asthma exacerbations during pregnancy is key to ensuring the safety of the mother and the fetus.
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January 2008

[Report on 16 rare species of human chromosomal].

Yi Chuan 2005 Mar;27(2):201-4

Liaoning Open Laboratory of Reproductive Medicine, Shenyang 110031,China.

By examining the lymphocytic chromosomes of peripheral blood from patients with amenorrhea, spontaneous abortion and stillbirth history, .the 16 rare species of human chromosomal abnormal karyotypes were discovered. They were 46,XY,t(6;11)(q25;p15);46,XY,inv(3)(p25;q29);46,XY,t(7;18)(q10;p10);46,X,t(X;13)(q24;q14);46,XY,t(4;7)(q33;q22);46,XY,t(8;15)(q24;q15);46,XY,t(2;17)(q33;q25);46,XX,t(4;7)(q34;q11);46,XX,t(1;3)(p36;p23);46,XX,t(4;6)(q35;p11);46,X,inv(X)(q22;q28);46,XX,t(7;10)(p11;q26);46,XX,t(3;6)(p21;q23);46,XX,t(8;16)(p21;p13);46,XX,t(8;9)(q21;q34);46,XY,t(17;22)(q21;q11). Their clinical situation were described. Discussion on the relationship between the chromosomal aberrations and phenotype effect indicates the importance of chromosome karyotyping in patients with abnormal reproductive history.
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March 2005

[Effect of estradiol and progesterone on production of macrophage colony-stimulating factor by human granulosa cells in vitro].

Zhonghua Fu Chan Ke Za Zhi 2003 Sep;38(9):549-51

Department of Gynecology, Women's Hospital, School of Medicine, Zhejiang University, Hanzhou 310006, China.

Objective: To investigate the effect of estradiol (E(2)) and progesterone (P) on macrophage colony-stimulating factor (M-CSF) production by human luteinized granulosa cells in vitro.

Methods: Human luteinized granulosa cells were isolated from follicular fluid of superovulated infertile patients undergoing intracytoplasmatic sperm injection (ICSI). Luteinized granulosa cells were cultured with HAM's F-10 medium with various concentrations of E(2) or P (0, 1 x 10(-7), 1 x 10(-6), 1 x 10(-5), 1 x 10(-4), 1 x 10(-3) mol/L). Media were removed at 72 h after culture. M-CSF in media was measured by a solid phase enzyme-linked immunosorbant assay (ELISA) and estradiol and progesterone in media were measured by enzyme immunoassays (EIA).

Results: The basic concentration of M-CSF in cultivated granulosa cells without E(2) stimulation was low (47 +/- 15 ng/L). However, E(2) at concentrations of 1 x 10(-6) - 1 x 10(-3) mol/L caused a significant increase of M-CSF secretion by luteinized granulosa cells in a dose-dependent mode (2.3, 4.5, 6.9, 7.9 times higher than the basic level, respectively) (P < 0.05). E(2) at a concentration of 1 x 10(-7) mol/L, however, did not stimulate the production of M-CSF (P > 0.05). P at concentrations of 1 x 10(-7) - 1 x 10(-3) mol/L showed no effect on M-CSF production by luteinized granulosa cells (P > 0.05).

Conclusions: Estradiol can stimulate M-CSF production by luteinized granulosa cells in vitro in a dose-dependent mode, on the other hand P cannot induce M-CSF production by luteinized granulosa cells. The mechanism of estradiol regulating follicular development may partially via M-CSF/receptor pathway.
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September 2003
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