Publications by authors named "Zhi Yu"

230 Publications

Manual Acupuncture at ST37 Modulates TRPV1 in Rats with Acute Visceral Hyperalgesia via Phosphatidylinositol 3-Kinase/Akt Pathway.

Evid Based Complement Alternat Med 2021 4;2021:5561999. Epub 2021 Oct 4.

Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210029, China.

Acupuncture can significantly ameliorate inflammatory pain in acute visceral hyperalgesia. Hyperalgesia is attenuated by inflammatory mediators that activate transient receptor potential vanilloid 1 (TRPV1), and TRPV1 is regulated by nerve growth factor (NGF)-induced phosphatidylinositol 3-kinase (PI3K)/Akt pathway. However, it is unknown whether NGF-induced PI3K/Akt pathway is associated with manual acupuncture (MA). In this study, the effect and mechanism of MA at Shangjuxu (ST37) and Quchi (LI11) were examined using an acetic acid-induced rat model with visceral hyperalgesia. We demonstrated that MA at ST37 significantly decreased abdominal withdrawal reflex (AWR) scores, proinflammatory cytokine expression (TNF-, IL-1, and IL-6), and TRPV1 protein and mRNA expression in rats with acute visceral hyperalgesia compared with the untreated controls, while MA at LI11 showed no effect. The effects of MA at ST37 were reversed after treatment with the PI3K agonist IGF-1 30 min before MA. In rats with visceral hyperalgesia, the upregulation of NGF, tropomyosin-receptor-kinase A (TrkA), PI3K, and phosphorylation-Akt (p-Akt) was decreased by MA at ST37, indicating that TRPV1 regulation via the NGF-induced PI3K/Akt pathway plays a vital role in the effects of MA-mediated amelioration of acute visceral hyperalgesia.
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http://dx.doi.org/10.1155/2021/5561999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505093PMC
October 2021

Rapid field trace detection of pesticide residue in food based on surface-enhanced Raman spectroscopy.

Mikrochim Acta 2021 10 7;188(11):370. Epub 2021 Oct 7.

College of Horticulture & Forestry Sciences, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.

Surface-enhanced Raman spectroscopy is an alternative detection tool for monitoring food security. However, there is still a lack of a conclusion of SERS detection with respect to pesticides and real sample analysis, and the summary of intelligent algorithms in SERS is also a blank. In this review, a comprehensive report of pesticides detection using SERS technology is given. The SERS detection characteristics of different types of pesticides and the influence of substrate on inspection are discussed and compared by the typical ways of classification. The key points, including the progress in real sample analysis and Raman data processing methods with intelligent algorithm, are highlighted. Lastly, major challenges and future research trends of SERS analysis of pesticide residue are also addressed. SERS has been proven to be a powerful technique for rapid test of residue pesticides in complex food matrices, but there still is a tremendous development space for future research.
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http://dx.doi.org/10.1007/s00604-021-05025-3DOI Listing
October 2021

Oxidation ruled transition from normal to anomalous periodic structures with femtosecond laser irradiation on Cr/Si films.

Opt Express 2021 Sep;29(20):31408-31417

Elucidation of the underlying physics for laser-induced periodic surface structures (LIPSSs) is of great importance for their controllable fabrication. We here demonstrate a periodic structure transition from normal to anomalous morphology, upon femtosecond laser irradiation on 50-nm thick Cr/Si films in an air pressure-tunable chamber. As the air pressure gradually decreases, the amount of surface oxide induced by preceding laser pulses is found to reduce, and eventually triggering the structure evolution from the anomalously oriented subwavelength to normally oriented deep-subwavelength LIPSSs. The intriguing structure transition is explained in terms of the competitive excitation between the transverse-electric scattered surface wave and transverse-magnetic hybrid plasmon wave, which is ruled by the thickness of the preformed oxide layer indeed.
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http://dx.doi.org/10.1364/OE.433035DOI Listing
September 2021

Polygenic Risk Scores for Kidney Function and Their Associations with Circulating Proteome, and Incident Kidney Diseases.

J Am Soc Nephrol 2021 Sep 21. Epub 2021 Sep 21.

J Coresh, Department of Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, United States.

Genome-wide association studies (GWAS) have revealed numerous loci for kidney function (estimated glomerular filtration rate, eGFR). The relationship of polygenic predictors of eGFR, risk of incident adverse kidney outcomes, and the plasma proteome is not known. We developed a genome-wide polygenic risk score (PRS) for eGFR by applying the LDpred algorithm to summary statistics generated from a multiethnic meta-analysis of CKDGen Consortium GWAS (N=765,348) and UK Biobank GWAS (90% of the cohort; N=451,508), followed by best parameter selection using the remaining 10% of UK Biobank (N=45,158). We then tested the association of the PRS in the Atherosclerosis Risk in Communities (ARIC) study (N=8,866) with incident chronic kidney disease, kidney failure, and acute kidney injury. We also examined associations between the PRS and 4,877 plasma proteins measured at at middle age and older adulthood and evaluated mediation of PRS associations by eGFR. The developed PRS showed significant associations with all outcomes with hazard ratios (95% CI) per 1 SD lower PRS ranged from 1.06 (1.01, 1.11) to 1.33 (1.28, 1.37). The PRS was significantly associated with 132 proteins at both time points. The strongest associations were with cystatin-C, collagen alpha-1(XV) chain, and desmocollin-2. Most proteins were higher at lower kidney function, except for 5 proteins including testican-2. Most correlations of the genetic PRS with proteins were mediated by eGFR. A PRS for eGFR is now sufficiently strong to capture risk for a spectrum of incident kidney diseases and broadly influences the plasma proteome, primarily mediated by eGFR.
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http://dx.doi.org/10.1681/ASN.2020111599DOI Listing
September 2021

Proteins Associated with Risk of Kidney Function Decline in the General Population.

J Am Soc Nephrol 2021 Sep;32(9):2291-2302

Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Background: Proteomic profiling may allow identification of plasma proteins that associate with subsequent changesin kidney function, elucidating biologic processes underlying the development and progression of CKD.

Methods: We quantified the association between 4877 plasma proteins and a composite outcome of ESKD or decline in eGFR by ≥50% among 9406 participants in the Atherosclerosis Risk in Communities (ARIC) Study (visit 3; mean age, 60 years) who were followed for a median of 14.4 years. We performed separate analyses for these proteins in a subset of 4378 participants (visit 5), who were followed at a later time point, for a median of 4.4 years. For validation, we evaluated proteins with significant associations (false discovery rate <5%) in both time periods in 3249 participants in the Chronic Renal Insufficiency Cohort (CRIC) and 703 participants in the African American Study of Kidney Disease and Hypertension (AASK). We also compared the genetic determinants of protein levels with those from a meta-analysis genome-wide association study of eGFR.

Results: In models adjusted for multiple covariates, including baseline eGFR and albuminuria, we identified 13 distinct proteins that were significantly associated with the composite end point in both time periods, including TNF receptor superfamily members 1A and 1B, trefoil factor 3, and -trace protein. Of these proteins, 12 were also significantly associated in CRIC, and nine were significantly associated in AASK. Higher levels of each protein associated with higher risk of 50% eGFR decline or ESKD. We found genetic evidence for a causal role for one protein, lectin mannose-binding 2 protein (LMAN2).

Conclusions: Large-scale proteomic analysis identified both known and novel proteomic risk factors for eGFR decline.
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http://dx.doi.org/10.1681/ASN.2020111607DOI Listing
September 2021

Simultaneously enhancing the ultimate strength and ductility of high-entropy alloys via short-range ordering.

Nat Commun 2021 Aug 16;12(1):4953. Epub 2021 Aug 16.

Institute of High Performance Computing, A*STAR, Singapore, Singapore.

Simultaneously enhancing strength and ductility of metals and alloys has been a tremendous challenge. Here, we investigate a CoCuFeNiPd high-entropy alloy (HEA), using a combination of Monte Carlo method, molecular dynamic simulation, and density-functional theory calculation. Our results show that this HEA is energetically favorable to undergo short-range ordering (SRO), and the SRO leads to a pseudo-composite microstructure, which surprisingly enhances both the ultimate strength and ductility. The SRO-induced composite microstructure consists of three categories of clusters: face-center-cubic-preferred (FCCP) clusters, indifferent clusters, and body-center-cubic-preferred (BCCP) clusters, with the indifferent clusters playing the role of the matrix, the FCCP clusters serving as hard fillers to enhance the strength, while the BCCP clusters acting as soft fillers to increase the ductility. Our work highlights the importance of SRO in influencing the mechanical properties of HEAs and presents a fascinating route for designing HEAs to achieve superior mechanical properties.
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http://dx.doi.org/10.1038/s41467-021-25264-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368001PMC
August 2021

Changes of fungal community and non-volatile metabolites during pile-fermentation of dark green tea.

Food Res Int 2021 09 31;147:110472. Epub 2021 May 31.

Key Laboratory of Horticulture Plant Biology, Ministry of Education, College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei 430070, People's Republic of China; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture, Wuhan, Hubei 430070, People's Republic of China. Electronic address:

Fungal community and non-volatile metabolites changes during the pile-fermentation are key factors to organoleptic qualities of dark green tea. However, the correlation between fungal succession and non-volatile compounds has never been satisfactorily explained. The purpose of the present study was to investigate fungal succession and its correlation with flavor compounds by multi-omics. Illumina Miseq sequencing of ITS1 region was conducted to analyze the fungal succession, a total of 78 OTUs which consisted of one phyla, nine classes, 15 orders, 26 families, 37 genera were identified, with Ascomycota as dominant phyla. Cluster analysis and non-metric multidimensional scaling of samples demonstrated the distribution of OTUs in multi-dimensional space, the pile-fermentation process of dark green tea can be divided into four periods according to the generated trajectory of fungal population, S0, S1-S3, S4-S5, and S6. Aspergillus is the dominant genus. Penicillium, Cyberlindnera, Debaryomyces, Candida, Thermomyces, Rasamsonia, Thermoascus, and Byssochlamys appear in different periods. three alkaloids, seven catechins, nine amino acids, five organic acids, five flavones and flavonoid glycosides were identified by UPLC-QTOF-MS/MS, and the contents were all decreasing. Caffeine, EGC, EGCG, L-theanine, kaempferitrin, L-phenylalanine, gallic acid, and myricetin-3-O-galactoside are important ingredients which contribute to the flavor of dark green tea. This study demonstrated the fungal succession, non-volatile flavor compounds and their relationships during pile-fermentation of dark green tea, and provides new insights into evaluating pivotal role of fungal succession in the manufacturing process of dark green tea.
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http://dx.doi.org/10.1016/j.foodres.2021.110472DOI Listing
September 2021

Electroacupuncture Alleviates Diabetic Peripheral Neuropathy by Regulating Glycolipid-Related GLO/AGEs/RAGE Axis.

Front Endocrinol (Lausanne) 2021 6;12:655591. Epub 2021 Jul 6.

Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, Nanjing, China.

Diabetic peripheral neuropathy (DPN) is one of the most common complications of diabetes mellitus (DM) and affects over one-third of all patients. Neuropathic pain and nerve dysfunction induced by DM is related to the increase of advanced glycation end products (AGEs) produced by reactive dicarbonyl compounds in a hyperglycemia environment. AGEs induce the expression of pro-inflammatory cytokines the main receptor (RAGE), which has been documented to play a crucial role in the pathogenesis of diabetic peripheral neuropathy. Electroacupuncture (EA) has been reported to have a positive effect on paralgesia caused by various diseases, but the mechanism is unclear. In this study, we used high-fat-fed low-dose streptozotocin-induced rats as a model of type 2 diabetes (T2DM). Persistent metabolic disorder led to mechanical and thermal hyperalgesia, as well as intraepidermal nerve fiber density reduction and nerve demyelination. EA improved neurological hyperalgesia, decreased the pro-inflammatory cytokines, reduced the generation of AGEs and RAGE, and regulated the glyoxalase system in the EA group. Taken together, our study suggested that EA plays a role in the treatment of T2DM-induced DPN, and is probably related to the regulation of metabolism and the secondary influence on the GLO/AGE/RAGE axis.
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http://dx.doi.org/10.3389/fendo.2021.655591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290521PMC
July 2021

and , Two New Records on in China.

Mycobiology 2021 17;49(3):267-274. Epub 2021 May 17.

Institute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, China.

Two species isolated from fruit of in China were characterized based on morphology and multilocus phylogeny of ITS, , and gene sequences. The phylogeny indicated that the two species match and . A critical examination of phenotypic characteristics confirmed the phylogenetic results. was morphologically characterized by producing Alpha conidia with tapering toward both ends. Meanwhile, produced cylindrical or ellipsoidal Alpha conidia with two oil drops. Pathogenicity tests revealed that both species were pathogenic to fruit of . To our knowledge, the two species were firstly reported on in China.
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http://dx.doi.org/10.1080/12298093.2021.1912254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259869PMC
May 2021

Electroacupuncture at ST36 Improve the Gastric Motility by Affecting Neurotransmitters in the Enteric Nervous System in Type 2 Diabetic Rats.

Evid Based Complement Alternat Med 2021 16;2021:6666323. Epub 2021 Jun 16.

Nanjing University of Traditional Chinese Medicine, Nanjing 210023, Jiangsu, China.

Electroacupuncture (EA) can effectively relieve hyperglycemia and gastric emptying disorders in diabetic gastroparesis (DGP). However, the effect of EA on type 2 diabetes mellitus (T2DM) gastroparesis and its mechanism in the enteric nervous system (ENS) are rarely studied. We investigated the therapeutic effect of EA at ST36 and its effect on the main inhibitory and excitatory neurotransmitters in the ENS in DGP rats. Male Sprague-Dawley (SD) rats were fed a high-fat diet for 2 weeks and injected with streptozotocin (STZ) at 35 mg/kg to induce T2DM. T2DM rats were divided into the diabetic mellitus (DM) group and the EA group. The control (CON) group comprised normal rats without any intervention. EA treatment was started 6 weeks after the induction of DM and continued for 5 weeks. The body weight and food intake of the rats were recorded every week. Blood glucose, insulin, glucose tolerance, gastric emptying, and antral motility were measured after treatment. The expression of protein gene product 9.5 (PGP9.5), neuronal nitric oxide synthase (nNOS), and choline acetyltransferase (ChAT) in gastric antrum were quantified by western blotting and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The T2DM gastroparesis model was successfully established. EA treatment reduced the body weight, food intake, and blood glucose; improved glucose intolerance and insulin resistance; increased the gastric emptying rate, the mean antral pressure, and the amplitude of antral motility; and decreased the frequency of antral motility compared with those in the DM group. EA treatment increased the expression level of nNOS, ChAT, and PGP9.5 proteins, and nNOS and ChAT mRNA. The results suggested that EA at ST36 could ameliorate DGP, partly restore the damage to general neurons, and increase nNOS and ChAT in the gastric antrum. EA improved DGP partly via reducing the loss of inhibitory and excitatory neurotransmitters in the ENS.
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http://dx.doi.org/10.1155/2021/6666323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225438PMC
June 2021

Genomic and Chemical Investigation of Bioactive Secondary Metabolites From a Marine-Derived Fungus P2648.

Front Microbiol 2021 4;12:600991. Epub 2021 Jun 4.

Institute of Oceanography, Minjiang University, Fuzhou, China.

Marine fungi of the genus are rich resources of secondary metabolites, showing a variety of biological activities. Our anti-bacterial screening revealed that the crude extract from a coral-derived fungus P2648 showed strong activity against some pathogenic bacteria. Genome sequencing and mining uncovered that there are 28 secondary metabolite gene clusters in P2648, potentially involved in the biosynthesis of antibacterial compounds. Chemical isolation and structural determination suggested citrinin is the dominant component of the crude extracts of P2648, and our further tests confirmed that citrinin showed excellent activities against various pathogenic bacteria. Moreover, the gene cluster containing a homolog of the polyketide synthase CitS was identified as the citrinin biosynthesis gene cluster through genetic analysis. Interestingly, three isoquinoline alkaloids were unexpectedly activated and isolated from the mutant and structural determination by using high-resolution electron spray ionization mass spectroscopy (HRESIMS), 1D, and 2D NMR. Further antibacterial assays displayed that compounds 1 and 2, but not compound 3, showed moderate activities against two antibiotic-resistant pathogenic bacteria with minimum inhibitory concentration (MIC) of 16-32 μg/ml. In conclusion, our results demonstrated that citrinin and isoquinoline alkaloids represent as the major antibacterial agents in the coral-associated fungus P2648, and our genomic and chemical analyses present evidence in support of P2648 as a potent natural products source for anti-bacterial drug discovery.
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http://dx.doi.org/10.3389/fmicb.2021.600991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211754PMC
June 2021

Identification of nucleic acid aptamers against lactate dehydrogenase via SELEX and high-throughput sequencing.

Anal Bioanal Chem 2021 Jul 24;413(17):4427-4439. Epub 2021 May 24.

Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.

Nucleic acid aptamers are small fragments of DNA or RNA molecules binding specifically to targets, which can be obtained through in vitro screening via systematic evolution of ligands by exponential enrichment (SELEX). Lactate dehydrogenase (LDH) is an important tumor marker, whose level in patients is of great significance for diagnosis of many diseases. Here, we report the identification of LDH aptamers by 9 rounds of screening from a length-mixed single-stranded DNA library using the SELEX technology. After the 3rd and 7th rounds of aptamer screening, affinity was significantly improved, and fluorescence quantitative analysis showed stronger affinity for the aptamers selected from the 7th to 9th rounds of screening. After high-throughput sequencing, motif analysis, and secondary structure prediction, we finally chose and further investigated 15 candidate LDH aptamer sequences with obvious differences in secondary structure in the 7th to 9th rounds of screening. Among them, LDH7-1, LDH7-9, LDH8-2, and LDH9-1 were shown to bind to LDH protein with high affinity and specificity with K < 25 nM. This study provides new ideas for rapid detection of LDH protein content and enzyme activity, thus contributing to the development of rapid medical detection.
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http://dx.doi.org/10.1007/s00216-021-03397-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141549PMC
July 2021

Developing an Artificial Intelligence (A.I)-based descriptor of facial appearance that fits with the assessments of makeup experts.

Skin Res Technol 2021 May 17. Epub 2021 May 17.

ModiFace - A L'Oréal Group Company, Toronto, ON, Canada.

Objective: To develop an A.I-based automatic descriptor that detects and grades, from selfie pictures, 23 facial signs, hairs included, as a help to making-up procedures.

Material And Methods: The selfie images taken in very different conditions by 3326 women and men were used to create (90% of dataset) and validate (10% of dataset) a new algorithm architecture to appraise and grade 23 different facial signs such as lips, nose, eye color, eyebrows, eyelashes, and hair color as defined by makeup artists. Each selfie image was annotated by 12 experts and defined references to train Artificial Intelligence (A.I)-based algorithm.

Results: As some the 23 signs present a continuous or discontinuous feature, these were analyzed by two different statistical approaches. The results provided by the automatic descriptor system were not only in good agreement with the expert's assessments but were even found of a better precision and reproducibility. This automatic descriptor system has proven a good and robust accuracy despite the very variable conditions in the acquisition of selfie pictures.

Conclusion: Such automatic descriptor system seems providing a valuable help in making-up procedures and may extend to other activities such as Skincare or Haircare. As such it should allow large investigations to better evaluate the consumers' needs of esthetical improvements.
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http://dx.doi.org/10.1111/srt.13061DOI Listing
May 2021

Morphological and molecular identification of two new species (Ascomycota, Pleosporaceae) in section Radicina from China.

MycoKeys 2021 9;78:187-198. Epub 2021 Apr 9.

Institute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi, 530007, China Institute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests Nanning China.

The fungal genus was distributed widely and found in different habitats such as plant or indoor environment. During an investigation into this genus in China, two new species, and were respectively isolated from diseased leaves of and , which both belonged to Umbelliferae. Phylogenetically, they were determined as new species belonging in the section Radicina of based on the combined four gene fragments of ITS, , and . Morphologically, the two species were illustrated and compared with other relevant Alternaria species in section Radicina.
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http://dx.doi.org/10.3897/mycokeys.78.64853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052298PMC
April 2021

Urea as a By-Product of Ammonia Metabolism Can Be a Potential Serum Biomarker of Hepatocellular Carcinoma.

Front Cell Dev Biol 2021 1;9:650748. Epub 2021 Apr 1.

Department of Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Hepatocellular carcinoma (HCC) is highly malignant; nearly half of the new cases and deaths are in China. The poor prognosis of HCC is mainly due to late diagnosis; many new biomarkers have been developed for HCC diagnosis. However, few markers are quickly translated into clinical practice; early and differential diagnosis of HCC from cirrhosis and/or hepatitis is still a clinical challenge. Metabolomics and biochemical methods were used to reveal specific serum biomarkers of HCC. Most of the elevated metabolites in HCC and HBV patients were overlapped compared with controls. Urea was the specifically elevated serum biomarker of HCC patients. Moreover, urea combined with AFP and CEA can improve the sensitivity of HCC diagnosis. The plasma ammonia of HCC patients was significantly higher than healthy controls. Co-culture cell model revealed normal liver cells cooperated with cancer cells to metabolize ammonia into urea. The urea metabolism in cancer cells marginally depended on the expression of CPS1. However, the expression of CPS1 did not change with ammonium chloride, which might regulate the urea cycle through enzyme activity. The urea cycle could detoxify high concentrations of ammonia to promote cancer cell proliferation. Therefore, urea was a by-product of ammonia metabolism and could be a potential serum biomarker for HCC. The combined application of metabolomics and biochemical methods can discover new biomarkers for the early diagnosis of HCC and be quickly applied to clinical diagnosis.
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http://dx.doi.org/10.3389/fcell.2021.650748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047217PMC
April 2021

sp. nov., isolated from stream water in the Republic of Korea.

Int J Syst Evol Microbiol 2021 Apr;71(4)

Department of Life Science, Graduate School, Kyonggi University, 154-42 Gwanggyosan-ro Yeongtong-gu, Suwon-si, Gyeonggi-do 16227, Republic of Korea.

A novel bacterial strain, EJ-4, isolated from stream water collected at Seo-ho in Suwon, Republic of Korea, was characterized based on a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain EJ-4 belonged to the genus . The isolate is Gram-stain-negative, non-motile, aerobic, rod-shaped and forms pale yellow colonies on trypticase soy agar. The optimal growth of this strain was observed aerobically at 30 °C, pH 7 and 0.5 % NaCl. The major fatty acids were summed feature 3 (C 7 and/or C 6; 39.7 %) and C (32.0 %). The G+C content of strain EJ-4 was 58.4mol %. The average nucleotide identity and digital DNA-DNA hybridization values between strain EJ-4 and were 91.8 and 31.2 %, respectively. The major polar lipids detected in the isolate were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant isoprenoid quinone was ubiquinone-8. Based on the results of polyphasic taxonomic analysis of strain EJ-4, we describe a novel species of the genus , for which the name sp. nov. has been proposed, with EJ-4 (=KCTC 82074=JCM 34179=KEMB 1602-279) as the type strain.
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http://dx.doi.org/10.1099/ijsem.0.004681DOI Listing
April 2021

Novel green synthesis and characterization of a chemotherapeutic supplement by silver nanoparticles containing Berberis thunbergii leaf for the treatment of human pancreatic cancer.

Biotechnol Appl Biochem 2021 Apr 3. Epub 2021 Apr 3.

Department of General Surgery, Qinghai Province People's Hospital, Xining, Qinghai, China.

In recent years, silver nanoparticles have been used as modern chemotherapeutic drugs to treat several cancers such as pancreatic, breast, prostate, and blood cancers. No previous reports demonstrated the in vitro anti-human pancreatic cancer effects of the novel chemotherapeutic drug formulated by silver nanoparticles containing Berberis thunbergii leaf (AgNPs). The synthesized AgNPs were characterized using different techniques including UV-vis. and FT-IR spectroscopy, X-ray diffraction, scanning electron microscopy (SEM), and TEM. All techniques approved the synthesized silver nanoparticles. The SEM and TEM exhibited a uniform spherical morphology and an average size of about 15 nm for the biosynthesized nanoparticles, respectively. The 4-(dimethylamino)benzaldehyde,2,2-diphenyl-1- pikrilhydrazil (DPPH) test revealed similar antioxidant potentials for B. thunbergii leaf aqueous extract, AgNPs, and butylated hydroxytoluene. AgNPs inhibited half of the DPPH molecules in the concentration of 108 μg/mL. To survey the anti-human pancreatic cancer activities of AgNO , B. thunbergii leaf aqueous extract, and AgNPs, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used on common human pancreatic cancer cell lines. AgNPs had very low cell viability and anti-human pancreatic cancer effects dose-dependently against PANC-1, AsPC-1, and MIA PaCa-2. The IC50 values of the AgNPs were 259, 268, and 141 μg/mL against PANC-1, AsPC-1, and MIA PaCa-2 cell lines, respectively. It is thought that the AgNPs obtained can be used as an anticancer drug for the diagnosis of pancreatic cancer in humans after acceptance of the above findings in clinical study trials.
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http://dx.doi.org/10.1002/bab.2160DOI Listing
April 2021

Competitive adsorption of residual polyvinylpyrrolidone and detection molecular on flower liked silver nanoparticles.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 23;255:119717. Epub 2021 Mar 23.

College of Optical and Electronic Technology, China Jiliang University, 310018 Hangzhou, China. Electronic address:

The silver nanoparticles have been frequently used in SERS detection, for their unique optical properties and sensitive surface Raman enhancement properties. However, as the preparation of silver nanoparticles will use polyvinylpyrrolidone (PVP) to achieve the effect of reducing agent and surfactant, the surface of the prepared silver nanoparticles will be wrapped by PVP, forming an insulating layer and an ill-defined AgNPs interface, which limits the plasmonic coupling between the laminates of AgNPs. This paper reported a simple method to remove PVP for high performance and reusable SERS substrate, and the residue of PVP was studied after clean centrifugal by ethanol or water. When the number of cleaning times reached 10, there was basically no residual of PVP. The cleaned AgNPs interface effectively enhanced the plasma resonance of the local surface (LSPR) and greatly improved the SERS activity of the substrate. Moreover, probe molecules (R6G) are introduced to study the influence of single molecule PVP on subsequent detection. Through the competitive relationship between the two, it can be concluded that residual PVP has basically no influence on detection of the molecular which absorbed stronger than PVP, and the remaining PVP can be ignored.
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http://dx.doi.org/10.1016/j.saa.2021.119717DOI Listing
July 2021

Corrigendum: The GTPase-Activating Protein FgGyp1 Is Important for Vegetative Growth, Conidiation, and Virulence and Negatively Regulates DON Biosynthesis in .

Front Microbiol 2021 8;12:666050. Epub 2021 Mar 8.

Marine and Agricultural Biotechnology Laboratory, Institute of Oceanography, Minjiang University, Fuzhou, China.

[This corrects the article DOI: 10.3389/fmicb.2021.621519.].
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http://dx.doi.org/10.3389/fmicb.2021.666050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982732PMC
March 2021

Combination of Botulinum Toxin and minocycline Ameliorates Neuropathic Pain Through Antioxidant Stress and Anti-Inflammation via Promoting SIRT1 Pathway.

Front Pharmacol 2020 8;11:602417. Epub 2021 Mar 8.

Department of Neurology, Bethune First Hospital of Jilin University, Changchun, China.

Neuropathic pain (NP) is one of the intractable complications of spinal cord injury (SCI), with poor prognosis and seriously affects the quality of life of patients. This study aims to determine the treatment effect and mechanism of multimodal therapies in a rat model of SCI-induced NP by combining treatment with the anti-inflammatory agent minocycline (MC) and botulinum toxin (BoNT). The combined utilization alleviated SCI-induced NP and reduced apoptosis, inflammation, and oxidative stress of SCI by activating SIRT1 and dampening pAKT, P53, and p-NF-KB. BoNT with a concentration of 0.1 nm and MC with a concentration of 20 uM were selected for the experiment in the primary microglia and astrocytes treated with LPS. It was found that the combination of BoNT and MC obviously inhibits the inflammatory response and oxidative stress of glial cells, and notably activates SIRT1 and restrains pAKT, P53, and p-NF-KB. Therefore, in the treatment of SCI-induced NP, the combination of BoNT and MC markedly improves the therapeutic effect of NP by promoting the SIRT1 expression, thereby inactivating NF-KB, P53, and PI3K/AKT signaling pathway, inhibiting inflammation and oxidative stress as well as relieving SCI-induced NP.
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http://dx.doi.org/10.3389/fphar.2020.602417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982576PMC
March 2021

Machine-Learning-Assisted Autonomous Humidity Management System Based on Solar-Regenerated Super Hygroscopic Complex.

Adv Sci (Weinh) 2021 Mar 1;8(6):2003939. Epub 2021 Feb 1.

Department of Materials Science and Engineering National University of Singapore Singapore 117574 Singapore.

High levels of humidity can induce thermal discomfort and consequent health disorders. However, proper utilization of this astounding resource as a freshwater source can aid in alleviating water scarcity. Herein, a low-energy and highly efficient humidity control system is reported comprising of an in-house developed desiccant dehumidifier and hygrometer (sensor), with an autonomous operation capability that can realize simultaneous dehumidification and freshwater production. The high efficiency and energy saving mainly come from the deployed super hygroscopic complex (SHC), which exhibits high water uptake (4.64 g g) and facile regeneration. Machine-learning-assisted in-house developed low cost and high precision hygrometers enable the autonomous operation of the humidity management system. The dehumidifier can reduce the relative humidity (RH) of a confined room from 75% to 60% in 15 minutes with energy consumption of 0.05 kWh, saving more than 60% of energy compared with the commercial desiccant dehumidifiers, and harvest 10 L of atmospheric water in 24 h. Moreover, the reduction in RH from 80% to 60% at 32 °C results in the reduction of apparent temperature by about 7 °C, thus effectively improving the thermal comfort of the inhabitants.
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http://dx.doi.org/10.1002/advs.202003939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967090PMC
March 2021

The GTPase-Activating Protein FgGyp1 Is Important for Vegetative Growth, Conidiation, and Virulence and Negatively Regulates DON Biosynthesis in .

Front Microbiol 2021 21;12:621519. Epub 2021 Jan 21.

Marine and Agricultural Biotechnology Laboratory, Institute of Oceanography, Minjiang University, Fuzhou, China.

Ypt1 is a small Rab GTPase in yeast, Gyp1 functions at the Golgi as a negative regulator of Ypt1. Gyp1 homologs are conserved in filamentous fungi. However, the roles of Gyp1 in phytopathogenic fungi are still unclear. Herein, we investigated the functions of FgGyp1 in the wheat pathogen by live-cell imaging, genetic, and pathological analyses. Targeted gene replacement method was used to delete in . Phenotypic analyses showed that FgGyp1 is critically important not only for the vegetative growth of but also its conidiation. The mutant's vegetative growth was significantly reduced by 70% compared to the wild type PH-1. The virulence of deletion mutant was significantly decreased when compared with the wild type PH-1. We further found that FgGyp1 negatively regulates DON production of the fungus. Live-cell imaging clearly demonstrated that FgGyp1 mainly localizes to the Golgi apparatus. Moreover, the TBC domain, C-terminal, and N-terminal regions of FgGyp1 are found to be indispensable for its biological functions and normal localization. The Arg357 residue of FgGyp1 is essential for its functions but dispensable for the normal localization of the protein, while the Arg284 residue is not required for both the functions and normal localization of the protein. Furthermore, we showed that FgGyp1 essentially hydrolyzes the GTP-bound FgRab1 (activated form) to its corresponding GDP-bound (inactive) form , suggesting that FgGyp1 is a GTPase-activating protein (GAP) for FgRab1. Finally, FgGyp1 was found to be important for FgSnc1-mediated fusion of secretory vesicles from the Golgi with the plasma membrane in . Put together, these data demonstrate that FgGyp1 functions as a GAP for FgRab1 and is important for vegetative growth, conidiation and virulence, and negatively regulates DON biosynthesis in .
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http://dx.doi.org/10.3389/fmicb.2021.621519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858271PMC
January 2021

Electroacupuncture Regulates Inguinal White Adipose Tissue Browning by Promoting Sirtuin-1-Dependent PPAR Deacetylation and Mitochondrial Biogenesis.

Front Endocrinol (Lausanne) 2020 21;11:607113. Epub 2021 Jan 21.

Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Chinese Medicine, Nanjing, China.

Background: Previous studies had suggested that electroacupuncture (EA) can promote white adipose tissue (WAT) browning to counter obesity. But the mechanism was still not very clear.

Aim: In this study, we aim to study the effect of EA on promoting inguinal WAT (iWAT) browning and its possible mechanism.

Method: Three-week-old rats were randomly divided into a normal diet (ND) group and a high-fat diet (HFD) group. After 10 weeks, the HFD rats were grouped into HFD + EA group and HFD control group. Rats in the EA group were electro-acupunctured for 4 weeks on Tianshu (ST25) acupoint under gas anesthesia with isoflurane, while the rats in HFD group were under gas anesthesia only. Body weight and cumulative food intake were monitored, and H&E staining was performed to assess adipocyte area. The effect of EA on WAT was assessed by qPCR, immunoblotting, immunoprecipitation and Co-immunoprecipitation. Mitochondria were isolated from IWAT to observe the expression of mitochondrial transcription factor A (TFAM).

Results: The body weight, WAT/body weight ratio and cumulative food consumption obviously decreased (P < 0.05) in the EA group. The expressions of brown adipose tissue (BAT) markers were increased in the iWAT of EA rats. Nevertheless, the mRNA expressions of WAT genes were suppressed by 4-week EA treatment. Moreover, EA increased the protein expressions of SIRT-1, PPAR, PGC-1, UCP1 and PRDM16 which trigger the molecular conversion of iWAT browning. The decrease of PPAR acetylation was also found in EA group, indicating EA could advance WAT-browning through SIRT-1 dependent PPAR deacetylation pathway. Besides, we found that EA could activate AMPK to further regulate PGC-1-TFAM-UCP1 pathway to induce mitochondrial biogenesis.

Conclusion: In conclusion, EA can remodel WAT to BAT through inducing SIRT-1 dependent PPAR deacetylation, and regulating PGC-1-TFAM-UCP1 pathway to induce mitochondrial biogenesis. This may be one of the mechanisms by which EA affects weight loss.
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http://dx.doi.org/10.3389/fendo.2020.607113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859442PMC
May 2021

Improving two-stage thermophilic-mesophilic anaerobic co-digestion of swine manure and rice straw by digestate recirculation.

Chemosphere 2021 Jul 27;274:129787. Epub 2021 Jan 27.

Hunan Animal and Veterinary Science Institute, Changsha, 410131, China.

The anaerobic co-digestion (coAD) of swine manure (SM) and rice straw (RS) is appealing for renewable energy recovery and waste treatment worldwidely. Improving its performance is very important for its application. In this study, long-term semi-continuous experiments were conducted to evaluate the improving effects of digestate recirculation on the performance, energy recovery, and microbial community of two-stage thermophilic-mesophilic coAD of swine manure (SM) and rice straw (RS). The experimental results indicated that the coAD systems of SM and RS (mixing ratio of 3:1) with or without digestate recirculation could not realize phase separation. The reactors of both coAD systems were characterized by pH values ranging from 7.74 to 7.85, methane production as 0.41 ± 0.02 and 0.44 ± 0.03 L/L/d, and stable operation. Notably, digestate recirculation increased total methane production, organic matter removal, and reaction rate of the coAD system by 9.92 ± 5.08, 5.22 ± 1.94, and 9.73-12.60%, respectively. Digestate recirculation improved the performance of the coAD by significantly increasing the abundance of Methanosarcina (from 4.1% to 7.5%-10.7% and 35.7%) and decreasing that of Methanothermobacter (from 94.2% to 87.3%-83.6% and 56.8%). Thus, the main methanogenesis pathway of the coAD system was changed by digestate recirculation and the methane production was effectively improved. Although the energy input of the coAD system increased by 30.26%, digestate recirculation improved the energy balance of the total system by 6.83%.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129787DOI Listing
July 2021

PKM2 promotes cell metastasis and inhibits autophagy via the JAK/STAT3 pathway in hepatocellular carcinoma.

Mol Cell Biochem 2021 May 29;476(5):2001-2010. Epub 2021 Jan 29.

Digestive Department, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, No. 100 Hong Kong Road, Jiang'an District, Wuhan, 430015, China.

Pyruvate kinase M2 (PKM2) is a member of the pyruvate kinase family. It has been recently reported that PKM2 displays non-metabolic activities. Nevertheless, understanding of the role of PKM2 in hepatocellular carcinoma (HCC) is insufficient. Therefore, our study aimed at exploring the impact of PKM2 on malignant growth, autophagy as well as invasion in HCC. Expression of PKM2 in HCC specimens was examined by qRT-PCR and western blot. PKM2 knock down was generated in vitro by shRNA. Activities of malignant cells as well as downstream pathways were assessed. The MTT assay was carried out to evaluate HCC proliferation, and the FACS assay was conducted to study cell death. Elevated PKM2 levels were observed in HCC samples. Knockdown (KD) of PKM2 triggered apoptosis as well as autophagy and inhibited migration and proliferation of HCC cells. Furthermore, PKM2 KD reinforced JAK/STAT3 pathway stimulation. STAT3 inhibition counteracted the impact of PKM2 on proliferation, autophagy, migration as well as cell death in HCC. To conclude, the findings of our research suggest that PKM2 reinforced metastasis and inhibited autophagy in HCC through the JAK/STAT3 pathway, and that PKM2 could serve as a promising target for HCC treatment.
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http://dx.doi.org/10.1007/s11010-020-04041-wDOI Listing
May 2021

Intelligent spectral algorithm for pigments visualization, classification and identification based on Raman spectra.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Apr 29;250:119390. Epub 2020 Dec 29.

College of Optical and Electronic Technology, China Jiliang University, 310018 Hangzhou, China.

Raman spectroscopy is a molecular vibrational spectroscopic technique has developed rapidly in recent years, especially in rapid field detection. In this paper, we discuss the Raman spectral pretreatment method and classification algorithm by using nearly 300 pigments spectral data as an example. Here, more than 5 kinds of classification algorithms such as SVM, KNN, ANN and et al are used to sovle the problem of pigments visualization, classification and identification via Raman spectral, and the results show that most of the algorithms fit well, with an accuracy of 90%. Moreover, SNR (Signal to noise ratio) is introduced to evaluate the stability of our algorithm. When the SNR is low, the accuracy of the algorithm decreases sharply. When the SNR was 1, the accuracy rate reached the highest value of 39.46%. In order to slove this problem, the flattopwin, hanning, blackman algorithm was introduced to denoise the signal with low SNR, even when SNR = 1, the signal is 80% accurate. It is proved that in the extreme case of this application, the algorithm still maintains good accuracy, and our research pave the way to use interlligent algorithms to solve the problems in the fields of Raman spectral detection.
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http://dx.doi.org/10.1016/j.saa.2020.119390DOI Listing
April 2021

Controllable Self-Assembly of SERS Hotspots in Liquid Environment.

Langmuir 2021 01 4;37(2):939-948. Epub 2021 Jan 4.

College of Optical and Electronic Technology, China Jiliang University, 310018 Hangzhou, China.

Controllable synthesis of novel metal nanoparticles and effective capture of hotspots are of great significance for SERS (surface-enhanced Raman spectroscopy) detection. Therefore, in this paper, a green controllable synthesis method of gold nanoparticle was achieved via epigallocatechin gallate reduction. Different morphologies of gold nanoparticles were synthesized just by changing the solution pH values, and the growth kinetics of AuNPs (gold nanoparticles) were systematically studied. The synthetic AuNPs were put in a droplet to study dynamic variations of self-assembly SERS hotspots from the liquid sol state to the solid dry state. The addition of halogen ions in the droplet can controllably regulate the self-assembly three-dimensional hotspot model of gold nanoparticles in the evaporation process of a droplet, during which the most enhancement effect can be easily captured. The dynamically changing images of nanoparticles in the process were graphically described based on the internal interaction forces of a droplet. Two stronger areas in the changes of SERS intensity were achieved with a high concentration of halogen ions, while only one maximum intensity area was obtained with a low concentration of halogen ions added. This method can effectively avoid complex and unpredictable microenvironments of SERS substrates in the liquid drop, further improving the reproducibility of SERS detection as well as broadening it to biological applications.
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http://dx.doi.org/10.1021/acs.langmuir.0c03323DOI Listing
January 2021

Unravelling VO Diffusion Pathways CO Modification for High-Performance Zinc Ion Battery Cathode.

ACS Nano 2021 Jan 4;15(1):1273-1281. Epub 2021 Jan 4.

Department of Material Science and Engineering, National University of Singapore, Block E3A #03-14, 7 Engineering Drive 1, Singapore 117574, Singapore.

Vanadium-based oxide is widely investigated as a zinc ion battery (ZIB) cathode due to its ability to react reversibly with Zn. Despite its successful demonstration, modification with simple molecules has shown some promise in enhancing the performance of ZIBs. Thus, this presents an immense opportunity to explore simple molecules that can dramatically improve the electrochemical performance of electrodes. Thus, the effect of CO modification is studied in this work by decomposing oxalic acid within a hydrated VO framework. Based on the collective results, the presence of CO drastically lowers the relative energy of Zn diffusion through the pathways by forming weak electrostatic interactions between O and Zn. This leads to an enlarged diffusion contribution, which consequently results in enhanced stability and better rate performance. The as-synthesized CO-VO electrode delivers one of the highest specific capacities reported for vanadium-based oxides of . 471 mAh g. Furthermore, an excellent cyclic stability of 80% capacity retention after 4000 cycles at 2 A g is recorded for CO-VO, which suggests the importance of simple molecules in the material framework toward the enhancement of ZIB cathode performance.
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http://dx.doi.org/10.1021/acsnano.0c08432DOI Listing
January 2021

A first-principles study on strain engineering of monolayer stanene for enhanced catalysis of CO reduction.

Chemosphere 2021 Apr 15;268:129317. Epub 2020 Dec 15.

Institute of High Performance Computing, A∗STAR, Singapore.

Using first-principles calculations, we investigated the changes in the lattice structure, electronic structures and catalytic performance for CO reduction reaction (CORR) of stanene under applied strain. Our calculations showed that the initial buckled honeycomb structure of free-standing stanene becomes increasingly flat upon the increase of tensile strain. Stanene remains its gapless semiconductor characteristic within the strain range of -2% and 2%, beyond which a semiconductor-to-metal transition occurs. Under strain, the adsorption of CO is weakened, which can facilitate the desorption of product CO, enabling a strained stanene to be a better catalyst for CORR to CO than strain-free stanene. In particular, the stanene with 4% strain may give rise to the best performance because of the weakest CO adsorption (E= -0.15 eV). The adsorption of intermediate product COOH on stanene is tunable with strain. We also evaluated the overall catalytic performance of the strained stanene based on the adsorption of CO and COOH and the selectivity against HER. If the reduction of COOH is governed by adsorption of the intermediate, a 10% strain may give a stronger COOH adsorption, weaker CO adsorption and better selectivity against HER, leading to an enhanced catalytic performance for CORR to CO. On the other hand, if the reduction of COOH is governed by desorption, a tensile strain higher than 4% may result in an enhanced catalytic performance. Our study here suggests that strain-tuned stanene might serve as an optimal electrocatalyst for CORR to CO with a high activity and selectivity.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129317DOI Listing
April 2021
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