Publications by authors named "Zhi Yang"

1,024 Publications

  • Page 1 of 1

A Highly Specific Multiple Enhancement Theranostic Nanoprobe for PET/MRI/PAI Image-Guided Radioisotope Combined Photothermal Therapy in Prostate Cancer.

Small 2021 Apr 18:e2100378. Epub 2021 Apr 18.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), NMPA Key Laboratory for Research and Evaluation of Radiopharmaceuticals (National Medical Products Administration), Department of Nuclear Medicine, Peking University Cancer Hospital and Institute, Beijing, 100142, China.

An integrated molecular probe for combined tumor-targeted multimodal imaging and therapy in the era of precision medicine requires a multiplexed platform that simultaneously has high targeting specificity, versatile conjugation capability, and biocompatibility. Here, a novel biocompatible melanin nanoprobe (PMNs-II-813) coupled with a highly specific prostate-specific membrane antigen small molecule inhibitor is developed for the targeted multimodal diagnosis and treatment of prostate cancer. The melanin nanoparticles demonstrate photoacoustic imaging and photothermal therapy (PTT) functionalities via strong near-infrared absorption. The imaging contrast agents Zr and Mn are stably conjugated to the nanoparticles for positron emission tomography (PET) and magnetic resonance imaging (MRI). Fusion PET/MRI with PMNs-II-813 enables the monitoring of treatment effects in real time and lasts for more than 1 week, demonstrating the capability for multimodal theranostics in prostate cancer. Labeling with a therapeutic radionuclide, I, simultaneously endows the nanoprobe with the capability for radioisotope therapy (RIT) and PTT under triple-modal imaging guidance. Combined PTT and RIT has an inhibitory effect on prostate cancer growth (tumor inhibition rate of ≈93% 20 days after treatment), which is significantly better than that with the single treatment. Overall, it is believed that PMNs-II-813 has potential for clinical translation to treat prostate cancer.
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http://dx.doi.org/10.1002/smll.202100378DOI Listing
April 2021

Rescue of maternal immune activation-induced behavioral abnormalities in adult mouse offspring by pathogen-activated maternal T cells.

Nat Neurosci 2021 Apr 15. Epub 2021 Apr 15.

Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Maternal immune activation (MIA) induced by lipopolysaccharides or polyinosinic:polycytidylic acid injections can induce behavioral abnormalities in adult mouse offspring. Here, we used the soluble tachyzoite antigen from Toxoplasma gondii, a parasite that infects approximately two billion people, to induce MIA in mice. The adult male offspring showed autism-relevant behaviors and abnormal brain microstructure, along with a pro-inflammatory T-cell immune profile in the periphery and upregulation of interleukin-6 in brain astrocytes. We show that adoptive transfer of regulatory T (T) cells largely reversed these MIA-induced phenotypes. Notably, pathogen-activated maternal T cells showed greater rescue efficacy than those from control donors. Single-cell RNA sequencing identified and characterized a unique group of pathogen-activated T cells that constitute 32.6% of the pathogen-activated maternal T population. Our study establishes a new preclinical parasite-mimicking MIA model and suggests therapeutic potential of adoptive T cell transfer in neuropsychiatric disorders associated with immune alterations.
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http://dx.doi.org/10.1038/s41593-021-00837-1DOI Listing
April 2021

Trioctylphosphine-Assisted Pre-protection Low-Temperature Solvothermal Synthesis of Highly Stable CsPbBr/TiO Nanocomposites.

J Phys Chem Lett 2021 Apr 13:3786-3794. Epub 2021 Apr 13.

Centre for Micro and Nano Devices, Department of Physics, COMSATS Institute of Information Technology, Islamabad 44500, Pakistan.

Lead halide perovskite quantum dots (PQDs) are reported as a promising branch of perovskites, which have recently emerged as a field in luminescent materials research. However, before the practical applications of PQDs can be realized, the problem of poor stability has not yet been solved. Herein, we propose a trioctylphosphine (TOP)-assisted pre-protection low-temperature solvothermal synthesis of highly stable CsPbBr/TiO nanocomposites. Due to the protection of branched ligands and the lower temperature of shell formation, these TOP-modified CsPbBr PQDs are successfully incorporated into a TiO monolith without a loss of fluorescence intensity. Because the excellent nature of both parent materials is preserved in CsPbBr/TiO nanocomposites, it is found that the as-prepared CsPbBr/TiO nanocomposites not only display excellent photocatalytic activity but also yield improved PL stability, enabling us to build highly stable white light-emitting diodes and to photodegrade rhodamine B.
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http://dx.doi.org/10.1021/acs.jpclett.1c00693DOI Listing
April 2021

The effect of different dietary levels of sodium and chloride on performance, blood parameters and excreta quality in goslings at 29 to 70 days of age.

J Anim Physiol Anim Nutr (Berl) 2021 Apr 12. Epub 2021 Apr 12.

Yangzhou University, Yangzhou, China.

The purpose of this study was to ascertain the appropriate levels of dietary sodium (Na ) and chloride (Cl ) for 29- to 70-day-old goslings and to investigate the effects of different levels of Na and Cl on the growth performance, water consumption, blood parameters and excreta quality of goslings to provide a reference for the healthy production of goslings. In Experiment 1, a total of 432 29-day-old male Jiangnan White goslings were randomly allotted to nine treatments according to a 3 × 3 factorial design, with six pens containing eight birds per treatment. The goslings were fed diets with three concentrations of Na (0.10%, 0.15% and 0.20%) and three concentrations of Cl (0.15%, 0.20% and 0.25%). The experimental period was 42 days. In Experiment 2, a total of 24 70-day-old Jiangnan White goslings were selected for four treatments (0.10% Na  × 0.15% Cl ; 0.10% Na  × 0.25% Cl ; 0.20% Na  × 0.15% Cl and 0.20% Na  × 0.25% Cl ) and housed separately in metabolic cages. The faeces were collected for 3 consecutive days. In Experiment 1, the average daily feed intake (ADFI), average daily gain (ADG) and feed/gain (F/G) ratio of goslings were unaffected by the treatments. However, low levels of Na and Cl significantly reduced the water consumption of goslings in the later growth period (p < 0.05). The average water consumption of goslings fed with 0.10% Na  × 0.15% Cl was significantly lower than that of the goslings fed with 0.20% Na  × 0.25% Cl (56 days, 1304.2 ml vs. 1471.7 ml; 63 days, 1452.8 ml vs. 1610.8 ml; 70 days, 1540.0 ml vs. 1775.4 ml; p < 0.05). The interaction between Na and Cl (Na  × Cl ) had a significant impact on the blood haemoglobin (HGB) and haematocrit (HCT) levels in the goslings (p < 0.05). HGB increased linearly with increasing levels of Na . HGB and HCT first increased and then decreased with increasing levels of Cl . In Experiment 2, Na and Cl levels had significant effects on the excreta moisture content (p < 0.05). Goslings fed with 0.10% Na  × 0.15% Cl had a low moisture content of 5.58% compared to the goslings fed with 0.20% Na  × 0.25% Cl (87.51% vs. 93.09%; p < 0.05). The levels of dietary Na had a significant effect on the retention ratio of Na (p < 0.05), with the value for the 0.20% Na group being significantly higher than that for the 0.10% Na group (p < 0.05). In summary, different levels of Na and Cl did not affect the growth of goslings. To reduce the water consumption and moisture content of excreta, the Na and Cl levels in the diet can be as low as 0.10% and 0.15%, respectively.
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http://dx.doi.org/10.1111/jpn.13541DOI Listing
April 2021

Yolk-Shelled Gold@Cuprous Oxide Nanostructures with Hot Carriers Boosting Photocatalytic Performance.

Langmuir 2021 Apr 8;37(15):4578-4586. Epub 2021 Apr 8.

Shanghai Key Laboratory of Molecular Imaging, Collaborative Innovation Center for Biomedicine, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China.

Plasmonic Au nanoparticles (NPs) have been commonly used to enhance the photocatalytic activity of CuO. Till now, core-shell Au NP@CuO composites have been reported in previous studies. Yet, these Au@CuO composites only exhibit visible light response. Other special Au nanostructures, such as Au nanorods (NRs) or Au nanobipyramids (NBPs), which possess near-infrared light absorption, were rarely used to endow the near-infrared light response for CuO. In this work, for the first time, we used Au NPs, Au NRs, and Au NBPs and employed a handy and universal method to synthesize a series of yolk-shelled Au@CuO composites. The results showed that the yolk-shelled Au@CuO composites had much higher photocatalytic activity than their solid-shelled ones and pure CuO. More importantly, yolk-shelled Au NR@CuO and Au NBP@CuO composites indeed presented excellent near-infrared light-driven photocatalytic activity, which were impossible for Au NP@CuO and pure CuO. This outstanding performance for yolk-shelled Au NR@CuO and Au NBP@CuO could be attributed to the transfer of abundant hot electrons from Au NRs or Au NBPs to CuO, and the timely utilization of hot holes on Au through the rich pore channels on their yolk-shelled structure. Furthermore, yolk-shelled Au@CuO also showed better stability than pure CuO, owing to the migration of the oxidizing holes from CuO to Au driven by the built-in electric field. This work may give a guide to fabricate controllable and effective photocatalysts based on plasmonic metals and semiconductors with full solar light-driven photocatalytic activities in the future.
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http://dx.doi.org/10.1021/acs.langmuir.1c00198DOI Listing
April 2021

Predicting mortality in acute ischaemic stroke treated with mechanical thrombectomy: analysis of a multicentre prospective registry.

BMJ Open 2021 Apr 1;11(4):e043415. Epub 2021 Apr 1.

Department of Neurology, Jinan University First Affiliated Hospital, Guangzhou, China

Objectives: We aimed to determine predictors of mortality within 90 days and develop a simple score for patients with mechanical thrombectomy (MT).

Design: Analysis of a multicentre prospective registry.

Setting: In six participating centres, patients who had an acute ischaemic stroke (AIS) treated by MT between March 2017 and May 2018 were documented prospectively.

Participants: 224 patients with AIS were treated by MT.

Results: Of 224 patients, 49 (21.9%) patients died, and 87 (38.8%) were independent. Variables associated with 90-day mortality were age, previous stroke, admission National Institutes of Health Stroke Scale (NIHSS), fasting blood glucose and occlusion site. Logistic regression identified four variables independently associated with 90-day mortality: age ≥80 years (OR 3.26, 95% CI 1.45 to 7.33), previous stroke (OR 2.33, 95% CI 1.04 to 5.21), admission NIHSS ≥18 (OR 2.37, 95% CI 1.13 to 4.99) and internal carotid artery or basilar artery occlusion (OR 2.92, 95% CI 1.34 to 6.40). Using these data, we developed predicting 90-day mortality of AIS with MT (PRACTICE) score ranging from 0 to 6 points. The receiver operator curve analysis found that PRACTICE score (area under the curve (AUC)=0.744, 95% CI 0.669 to 0.820) was numerically better than iScore (AUC=0.661, 95% CI 0.577 to 0.745) and Predicting Early Mortality of Ischemic Stroke score (AUC=0.638, 95% CI 0.551 to 0.725) for predicting 90-day mortality.

Conclusions: We developed a simple score to estimate the 90-day mortality of patients who had an AIS treated with MT. But the score needs to be prospectively validated.

Trial Registration Number: Chinese Clinical Trial Registry (ChiCTR-OOC-17013052).
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http://dx.doi.org/10.1136/bmjopen-2020-043415DOI Listing
April 2021

ICAM-1 orchestrates the abscopal effect of tumor radiotherapy.

Proc Natl Acad Sci U S A 2021 Apr;118(14)

Medical Isotopes Research Center, Department of Radiation Medicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China;

Compelling evidence indicates that radiotherapy (RT) has a systemic inhibitory effect on nonirradiated lesions (abscopal effect) in addition to the ablation of irradiated tumors. However, this effect occurs only in rare circumstances in clinical practice, and mechanisms underlying the abscopal effect of RT are neither fully understood nor therapeutically utilized. Here we identified that intercellular adhesion molecule-1 (ICAM-1), an inducible glycoprotein of the immunoglobulin superfamily, is up-regulated in nonirradiated tumors responsive to RT. ICAM-1 expression in preclinical animal models can be noninvasively detected by optical imaging and positron emission tomography (PET) using near-infrared fluorescence dye- and Cu-labeled imaging probes that we synthesized, respectively. Importantly, the expression levels of ICAM-1 determined by quantitative PET imaging showed a strong negative linear correlation with the growth of nonirradiated tumors. Moreover, genetic or pharmacologic up-regulation of ICAM-1 expression by either an intratumoral injection of engineered recombinant adenovirus or systemic administration of a Toll-like receptor 7 agonist-capsulated nanodrug could induce markedly increased abscopal responses to local RT in animal models. Mechanistic investigation revealed that ICAM-1 expression can enhance both the activation and tumor infiltration of CD8 T cells to improve the responses of the nonirradiated tumors to RT. Together, our findings suggest that noninvasive PET imaging of ICAM-1 expression could be a powerful means to predict the responses of nonirradiated tumors to RT, which could facilitate the exploration of new combination RT strategies for effective ablation of primary and disseminated lesions.
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http://dx.doi.org/10.1073/pnas.2010333118DOI Listing
April 2021

Observation of a Near-Threshold Structure in the K^{+} Recoil-Mass Spectra in e^{+}e^{-}→K^{+}(D_{s}^{-}D^{*0}+D_{s}^{*-}D^{0}).

Authors:
M Ablikim M N Achasov P Adlarson S Ahmed M Albrecht R Aliberti A Amoroso Q An Anita X H Bai Y Bai O Bakina R Baldini Ferroli I Balossino Y Ban K Begzsuren N Berger M Bertani D Bettoni F Bianchi J Biernat J Bloms A Bortone I Boyko R A Briere H Cai X Cai A Calcaterra G F Cao N Cao S A Cetin J F Chang W L Chang G Chelkov D Y Chen G Chen H S Chen M L Chen S J Chen X R Chen Y B Chen Z J Chen W S Cheng G Cibinetto F Cossio X F Cui H L Dai X C Dai A Dbeyssi R B de Boer D Dedovich Z Y Deng A Denig I Denysenko M Destefanis F De Mori Y Ding C Dong J Dong L Y Dong M Y Dong X Dong S X Du J Fang S S Fang Y Fang R Farinelli L Fava F Feldbauer G Felici C Q Feng M Fritsch C D Fu Y Fu Y Gao Y Gao Y Gao Y G Gao I Garzia E M Gersabeck A Gilman K Goetzen L Gong W X Gong W Gradl M Greco L M Gu M H Gu S Gu Y T Gu C Y Guan A Q Guo L B Guo R P Guo Y P Guo Y P Guo A Guskov T T Han X Q Hao F A Harris K L He F H Heinsius C H Heinz T Held Y K Heng C Herold M Himmelreich T Holtmann Y R Hou Z L Hou H M Hu J F Hu T Hu Y Hu G S Huang L Q Huang X T Huang Y P Huang Z Huang N Huesken T Hussain W Ikegami Andersson W Imoehl M Irshad S Jaeger S Janchiv Q Ji Q P Ji X B Ji X L Ji H B Jiang X S Jiang X Y Jiang Y Jiang J B Jiao Z Jiao S Jin Y Jin T Johansson N Kalantar-Nayestanaki X S Kang R Kappert M Kavatsyuk B C Ke I K Keshk A Khoukaz P Kiese R Kiuchi R Kliemt L Koch O B Kolcu B Kopf M Kuemmel M Kuessner A Kupsc M G Kurth W Kühn J J Lane J S Lange P Larin L Lavezzi Z H Lei H Leithoff M Lellmann T Lenz C Li C H Li Cheng Li D M Li F Li G Li H Li H Li H B Li H J Li J L Li J Q Li Ke Li L K Li Lei Li P L Li P R Li S Y Li W D Li W G Li X H Li X L Li Z Y Li H Liang H Liang Y F Liang Y T Liang L Z Liao J Libby C X Lin B J Liu C X Liu D Liu F H Liu Fang Liu Feng Liu H B Liu H M Liu Huanhuan Liu Huihui Liu J B Liu J Y Liu K Liu K Y Liu Ke Liu L Liu M H Liu Q Liu S B Liu Shuai Liu T Liu W M Liu X Liu Y Liu Y B Liu Z A Liu Z Q Liu X C Lou F X Lu H J Lu J D Lu J G Lu X L Lu Y Lu Y P Lu C L Luo M X Luo P W Luo T Luo X L Luo S Lusso X R Lyu F C Ma H L Ma L L Ma M M Ma Q M Ma R Q Ma R T Ma X N Ma X X Ma X Y Ma F E Maas M Maggiora S Maldaner S Malde Q A Malik A Mangoni Y J Mao Z P Mao S Marcello Z X Meng J G Messchendorp G Mezzadri T J Min R E Mitchell X H Mo Y J Mo N Yu Muchnoi H Muramatsu S Nakhoul Y Nefedov F Nerling I B Nikolaev Z Ning S Nisar S L Olsen Q Ouyang S Pacetti X Pan Y Pan A Pathak P Patteri M Pelizaeus H P Peng K Peters J Pettersson J L Ping R G Ping A Pitka R Poling V Prasad H Qi H R Qi K H Qi M Qi T Y Qi T Y Qi S Qian W B Qian Z Qian C F Qiao L Q Qin X S Qin Z H Qin J F Qiu S Q Qu K H Rashid K Ravindran C F Redmer A Rivetti V Rodin M Rolo G Rong Ch Rosner M Rump H S Sang A Sarantsev Y Schelhaas C Schnier K Schoenning M Scodeggio D C Shan W Shan X Y Shan M Shao C P Shen P X Shen X Y Shen B A Shi H C Shi R S Shi X Shi X D Shi W M Song Y X Song S Sosio S Spataro K X Su F F Sui G X Sun H K Sun J F Sun L Sun S S Sun T Sun W Y Sun X Sun Y J Sun Y K Sun Y Z Sun Z T Sun Y H Tan Y X Tan C J Tang G Y Tang J Tang J X Teng V Thoren I Uman C W Wang D Y Wang H J Wang H P Wang K Wang L L Wang M Wang M Z Wang Meng Wang W H Wang W P Wang X Wang X F Wang X L Wang Y Wang Y Wang Y D Wang Y F Wang Y Q Wang Z Wang Z Y Wang Ziyi Wang Zongyuan Wang D H Wei P Weidenkaff F Weidner S P Wen D J White U Wiedner G Wilkinson M Wolke L Wollenberg J F Wu L H Wu L J Wu X Wu Z Wu L Xia H Xiao S Y Xiao Y J Xiao Z J Xiao X H Xie Y G Xie Y H Xie T Y Xing G F Xu J J Xu Q J Xu W Xu X P Xu Y C Xu F Yan L Yan L Yan W B Yan W C Yan Xu Yan H J Yang H X Yang L Yang R X Yang S L Yang S L Yang Y H Yang Y X Yang Yifan Yang Zhi Yang M Ye M H Ye J H Yin Z Y You B X Yu C X Yu G Yu J S Yu T Yu C Z Yuan L Yuan W Yuan X Q Yuan Y Yuan Z Y Yuan C X Yue A Yuncu A A Zafar Y Zeng B X Zhang Guangyi Zhang H Zhang H H Zhang H Y Zhang J J Zhang J L Zhang J Q Zhang J W Zhang J Y Zhang J Z Zhang Jianyu Zhang Jiawei Zhang Lei Zhang S Zhang S F Zhang Shulei Zhang X D Zhang X Y Zhang Y Zhang Y H Zhang Y T Zhang Yan Zhang Yao Zhang Yi Zhang Z H Zhang Z Y Zhang G Zhao J Zhao J Y Zhao J Z Zhao Lei Zhao Ling Zhao M G Zhao Q Zhao S J Zhao Y B Zhao Y X Zhao Z G Zhao A Zhemchugov B Zheng J P Zheng Y Zheng Y H Zheng B Zhong C Zhong L P Zhou Q Zhou X Zhou X K Zhou X R Zhou A N Zhu J Zhu K Zhu K J Zhu S H Zhu T J Zhu W J Zhu X L Zhu Y C Zhu Z A Zhu B S Zou J H Zou

Phys Rev Lett 2021 Mar;126(10):102001

Institute of High Energy Physics, Beijing 100049, People's Republic of China.

We report a study of the processes of e^{+}e^{-}→K^{+}D_{s}^{-}D^{*0} and K^{+}D_{s}^{*-}D^{0} based on e^{+}e^{-} annihilation samples collected with the BESIII detector operating at BEPCII at five center-of-mass energies ranging from 4.628 to 4.698 GeV with a total integrated luminosity of 3.7  fb^{-1}. An excess of events over the known contributions of the conventional charmed mesons is observed near the D_{s}^{-}D^{*0} and D_{s}^{*-}D^{0} mass thresholds in the K^{+} recoil-mass spectrum for events collected at sqrt[s]=4.681  GeV. The structure matches a mass-dependent-width Breit-Wigner line shape, whose pole mass and width are determined as (3982.5_{-2.6}^{+1.8}±2.1)  MeV/c^{2} and (12.8_{-4.4}^{+5.3}±3.0)  MeV, respectively. The first uncertainties are statistical and the second are systematic. The significance of the resonance hypothesis is estimated to be 5.3  σ over the contributions only from the conventional charmed mesons. This is the first candidate for a charged hidden-charm tetraquark with strangeness, decaying into D_{s}^{-}D^{*0} and D_{s}^{*-}D^{0}. However, the properties of the excess need further exploration with more statistics.
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http://dx.doi.org/10.1103/PhysRevLett.126.102001DOI Listing
March 2021

Dynamic PET/CT Imaging of Ga-FAPI-04 in Chinese Subjects.

Front Oncol 2021 11;11:651005. Epub 2021 Mar 11.

Institute of Medical Technology, Peking University Health Science Center, Beijing, China.

This study aims to further explore dynamic Ga-FAPI-04 PET/CT imaging of healthy Chinese subjects and lung cancer patients. Moreover, the variability of Ga-FAPI-04 uptake in normal organs was measured to provide a basis for analyzing its biological distribution, interpreting auxiliary images, determining the reliability of image quantification, and monitoring treatment. Six patients (3 subjects without tumors and 3 lung cancer patients) separately underwent Ga-FAPI-04 and 2-[F]FDG PET/CT imaging within 1 week. The biodistribution and internal radiation dosimetry were reported and compared with data previously obtained from Caucasian patients. Moreover, the mean SUV (standardized uptake value) was normalized to body mass or to lean body mass (SUL), and the coefficients of variation (CVs) were calculated and compared for each volume of interest. The average whole-body effective dose was calculated to be 1.27E-02 mSv/MBq, which was comparable with previously reported results of Ga-FAPI-04 probes. Furthermore, the SUVmean was slightly higher than the SULmean in most organs; however, the CV of the SULmean for most organs was higher than that of the SUVmean at later time points. In the liver, the CV of the SUVmean was lower (12.7%) than that of the SULmean and was similar to the CV for corresponding 2-[F]FDG PET/CT value (11.8%). In addition, Ga-FAPI-04 PET/CT showed good efficacy for diagnosing lung cancer patients in this study. A comparison of the radiation dosimetry obtained before from a Caucasian population demonstrated no clinically significant differences between these two populations after Ga-FAPI-04 injection. The variability in most organs was slightly lower for SUVmean than for SULmean, suggesting that SUVmean may be the preferable parameter for quantifying images obtained with Ga-FAPI-04. In addition, Ga-FAPI-04 PET/CT imaging is expected to be a promising tool for diagnosing lung cancer.
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http://dx.doi.org/10.3389/fonc.2021.651005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991833PMC
March 2021

Acute Kidney Injury following Cardiopulmonary Bypass: A Challenging Picture.

Oxid Med Cell Longev 2021 9;2021:8873581. Epub 2021 Mar 9.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, 229 Taibai North Road, Xi'an 710069, China.

Recent studies have recognized several risk factors for cardiopulmonary bypass- (CPB-) associated acute kidney injury (AKI). However, the lack of early biomarkers for AKI prevents practitioners from intervening in a timely manner. We reviewed the literature with the aim of improving our understanding of the risk factors for CPB-associated AKI, which may increase our ability to prevent or improve this condition. Some novel early biomarkers for AKI have been introduced. In particular, a combinational use of these biomarkers would be helpful to improve clinical outcomes. Furthermore, we discuss several interventions that are aimed at managing CPB-associated AKI, may increase the effect of renal replacement therapy (RRT), and may contribute to preventing CPB-associated AKI. Collectively, the conclusions of this paper are limited by the availability of clinical trial evidence and conflicting definitions of AKI. A guideline is urgently needed for CPB-associated AKI.
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http://dx.doi.org/10.1155/2021/8873581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7963912PMC
March 2021

Model-Independent Determination of the Spin of the Ω^{-} and Its Polarization Alignment in ψ(3686)→Ω^{-}Ω[over ¯]^{+}.

Authors:
M Ablikim M N Achasov P Adlarson S Ahmed M Albrecht A Amoroso Q An Anita Y Bai O Bakina R Baldini Ferroli I Balossino Y Ban K Begzsuren J V Bennett N Berger M Bertani D Bettoni F Bianchi J Biernat J Bloms A Bortone I Boyko R A Briere H Cai X Cai A Calcaterra G F Cao N Cao S A Cetin J F Chang W L Chang G Chelkov D Y Chen G Chen H S Chen M L Chen S J Chen X R Chen Y B Chen W S Cheng G Cibinetto F Cossio X F Cui H L Dai J P Dai X C Dai A Dbeyssi R B de Boer D Dedovich Z Y Deng A Denig I Denysenko M Destefanis F De Mori Y Ding C Dong J Dong L Y Dong M Y Dong S X Du J Fang S S Fang Y Fang R Farinelli L Fava F Feldbauer G Felici C Q Feng M Fritsch C D Fu Y Fu X L Gao Y Gao Y Gao Y G Gao I Garzia E M Gersabeck A Gilman K Goetzen L Gong W X Gong W Gradl M Greco L M Gu M H Gu S Gu Y T Gu C Y Guan A Q Guo L B Guo R P Guo Y P Guo Y P Guo A Guskov S Han T T Han T Z Han X Q Hao F A Harris K L He F H Heinsius T Held Y K Heng M Himmelreich T Holtmann Y R Hou Z L Hou H M Hu J F Hu T Hu Y Hu G S Huang L Q Huang X T Huang Z Huang N Huesken T Hussain W Ikegami Andersson W Imoehl M Irshad S Jaeger S Janchiv Q Ji Q P Ji X B Ji X L Ji H B Jiang X S Jiang X Y Jiang J B Jiao Z Jiao S Jin Y Jin T Johansson N Kalantar-Nayestanaki X S Kang R Kappert M Kavatsyuk B C Ke I K Keshk A Khoukaz P Kiese R Kiuchi R Kliemt L Koch O B Kolcu B Kopf M Kuemmel M Kuessner A Kupsc M G Kurth W Kühn J J Lane J S Lange P Larin L Lavezzi H Leithoff M Lellmann T Lenz C Li C H Li Cheng Li D M Li F Li G Li H B Li H J Li J L Li J Q Li Ke Li L K Li Lei Li P L Li P R Li S Y Li W D Li W G Li X H Li X L Li Z B Li Z Y Li H Liang H Liang Y F Liang Y T Liang L Z Liao J Libby C X Lin B Liu B J Liu C X Liu D Liu D Y Liu F H Liu Fang Liu Feng Liu H B Liu H M Liu Huanhuan Liu Huihui Liu J B Liu J Y Liu K Liu K Y Liu Ke Liu L Liu Q Liu S B Liu Shuai Liu T Liu X Liu Y B Liu Z A Liu Z Q Liu Y F Long X C Lou F X Lu H J Lu J D Lu J G Lu X L Lu Y Lu Y P Lu C L Luo M X Luo P W Luo T Luo X L Luo S Lusso X R Lyu F C Ma H L Ma L L Ma M M Ma Q M Ma R Q Ma R T Ma X N Ma X X Ma X Y Ma Y M Ma F E Maas M Maggiora S Maldaner S Malde Q A Malik A Mangoni Y J Mao Z P Mao S Marcello Z X Meng J G Messchendorp G Mezzadri T J Min R E Mitchell X H Mo Y J Mo N Yu Muchnoi H Muramatsu S Nakhoul Y Nefedov F Nerling I B Nikolaev Z Ning S Nisar S L Olsen Q Ouyang S Pacetti X Pan Y Pan A Pathak P Patteri M Pelizaeus H P Peng K Peters J Pettersson J L Ping R G Ping A Pitka R Poling V Prasad H Qi H R Qi M Qi T Y Qi S Qian W-B Qian Z Qian C F Qiao L Q Qin X P Qin X S Qin Z H Qin J F Qiu S Q Qu K H Rashid K Ravindran C F Redmer A Rivetti V Rodin M Rolo G Rong Ch Rosner M Rump A Sarantsev Y Schelhaas C Schnier K Schoenning D C Shan W Shan X Y Shan M Shao C P Shen P X Shen X Y Shen H C Shi R S Shi X Shi X D Shi J J Song Q Q Song W M Song Y X Song S Sosio S Spataro F F Sui G X Sun J F Sun L Sun S S Sun T Sun W Y Sun Y J Sun Y K Sun Y Z Sun Z T Sun Y H Tan Y X Tan C J Tang G Y Tang J Tang V Thoren B Tsednee I Uman B Wang B L Wang C W Wang D Y Wang H P Wang K Wang L L Wang M Wang M Z Wang Meng Wang W H Wang W P Wang X Wang X F Wang X L Wang Y Wang Y Wang Y D Wang Y F Wang Y Q Wang Z Wang Z Y Wang Ziyi Wang Zongyuan Wang D H Wei P Weidenkaff F Weidner S P Wen D J White U Wiedner G Wilkinson M Wolke L Wollenberg J F Wu L H Wu L J Wu X Wu Z Wu L Xia H Xiao S Y Xiao Y J Xiao Z J Xiao X H Xie Y G Xie Y H Xie T Y Xing X A Xiong G F Xu J J Xu Q J Xu W Xu X P Xu L Yan L Yan W B Yan W C Yan Xu Yan H J Yang H X Yang L Yang R X Yang S L Yang Y H Yang Y X Yang Yifan Yang Zhi Yang M Ye M H Ye J H Yin Z Y You B X Yu C X Yu G Yu J S Yu T Yu C Z Yuan W Yuan X Q Yuan Y Yuan Z Y Yuan C X Yue A Yuncu A A Zafar Y Zeng B X Zhang Guangyi Zhang H H Zhang H Y Zhang J L Zhang J Q Zhang J W Zhang J Y Zhang J Z Zhang Jianyu Zhang Jiawei Zhang L Zhang Lei Zhang S Zhang S F Zhang T J Zhang X Y Zhang Y Zhang Y H Zhang Y T Zhang Yan Zhang Yao Zhang Yi Zhang Z H Zhang Z Y Zhang G Zhao J Zhao J Y Zhao J Z Zhao Lei Zhao Ling Zhao M G Zhao Q Zhao S J Zhao Y B Zhao Y X Zhao Zhao Z G Zhao A Zhemchugov B Zheng J P Zheng Y Zheng Y H Zheng B Zhong C Zhong L P Zhou Q Zhou X Zhou X K Zhou X R Zhou A N Zhu J Zhu K Zhu K J Zhu S H Zhu W J Zhu X L Zhu Y C Zhu Z A Zhu B S Zou J H Zou

Phys Rev Lett 2021 Mar;126(9):092002

Institute of High Energy Physics, Beijing 100049, People's Republic of China.

We present an analysis of the process ψ(3686)→Ω^{-}Ω[over ¯]^{+} (Ω^{-}→K^{-}Λ, Ω[over ¯]^{+}→K^{+}Λ[over ¯], Λ→pπ^{-}, Λ[over ¯]→p[over ¯]π^{+}) based on a dataset of 448×10^{6}  ψ(3686) decays collected with the BESIII detector at the BEPCII electron-positron collider. The helicity amplitudes for the process ψ(3686)→Ω^{-}Ω[over ¯]^{+} and the decay parameters of the subsequent decay Ω^{-}→K^{-}Λ (Ω[over ¯]^{+}→K^{+}Λ[over ¯]) are measured for the first time by a fit to the angular distribution of the complete decay chain, and the spin of the Ω^{-} is determined to be 3/2 for the first time since its discovery more than 50 years ago.
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http://dx.doi.org/10.1103/PhysRevLett.126.092002DOI Listing
March 2021

Potential application value of PET/computed tomography in retroperitoneal leiomyosarcoma and a literature review.

Nucl Med Commun 2021 Mar 18. Epub 2021 Mar 18.

Department of Nuclear Medicine, Chifeng Municipal Hospital, Inner Mongolia Medical University, Inner Mongolia Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, China.

Objective: To analyze the fluorine-18 fludeoxyglucose PET/computed tomography (18F-FDG PET/CT) findings of retroperitoneal leiomyosarcoma (RLMS) and the role of this method in differentiating between benign and malignant masses and classifying the malignant degree to improve the understanding of this rare disease.

Methods: Eight leiomyomas (A group), 13 RLMSs (B group), and 20 postoperative recurrence/metastasis RLMSs (C group) were enrolled. PET/CT features of B group were analyzed. The differences of metabolic parameters between three groups were compared, receiver operating characteristic (ROC) curve analysis was performed to group A and B, and correlation analysis was performed to subgroup B.

Results: (1) The RLMS patients were more likely to be female, and PET/CT showed a high degree of heterogeneous metabolism in the soft tissue mass. (2) The standardized uptake value (SUV) of RLMS were significantly higher than those of benign leiomyomas (P < 0.05). The area under the ROC curve was 0.909, the sensitivity and specificity for diagnosing RLMS were 0.923 and 0.750, respectively, The SUVmax and SUVstd of primary RLMS were moderately associated with the Ki67 index. The mean SUVmax in the G1, G2 and G3 subgroups increased successively (4.15 ± 0.35, 6.47 ± 0.83, and 10.13 ± 4.29, respectively). (3) Primary RLMS was characterized by local invasion, but hematogenous metastasis and lymph node metastasis were rare. Postoperative recurrence/metastasis of RLMS was characterized by local recurrence and hematogenous metastasis, but lymph node metastasis was rare.

Conclusion: PET/CT has potential value in the preoperative staging, benign and malignant differentiation, malignant degree classification and postoperative follow-up of RLMS.
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http://dx.doi.org/10.1097/MNM.0000000000001388DOI Listing
March 2021

Zeolite-Based Memristive Synapse with Ultralow Sub-10-fJ Energy Consumption for Neuromorphic Computation.

Small 2021 Apr 18;17(13):e2006662. Epub 2021 Mar 18.

Key Laboratory for UV Light-Emitting Materials and Technology (Northeast Normal University), Ministry of Education, 5268 Renmin Street, Changchun, 130024, P. R. China.

The development of neuromorphic computation faces the appreciable challenge of implementing hardware with energy consumption on the level of a femtojoule per synaptic event to be comparable with the energy consumption of human brain. Controllable ultrathin conductive filaments are needed to achieve such extremely low energy consumption in memristive synapses but their formation is difficult to control owing to their stochastic morphology and unexpected overgrowth. Herein, a zeolite-based memristive synapse is demonstrated for the first time, in which Ag exchange in the sub-nanometer pore closely resembles synaptic Ca dynamics across biomembrane channel. Particularly, the confined ultrasmall pore and low Ag ion migration barrier give the zeolite-based memristive synapse ultralow energy consumption below 10 fJ per synaptic spike, on par with the biological counterpart. Experimental results reveal that the gradual memristive effect is attributed to the dimension modulation of Ag clusters. In addition to emulating inherent cognitive functions through electrical stimulations, the experience-dependent transition of short-term plasticity to long-term plasticity using a chemical modulation method is achieved by treating the initial Ag quantity as a learning experience. The proposed memristors can be used to develop highly efficient memristive neural networks and are considered as a candidate for application in neuromorphic computation.
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http://dx.doi.org/10.1002/smll.202006662DOI Listing
April 2021

Highlight selection of radiochemistry and radiopharmacy developments by editorial board.

EJNMMI Radiopharm Chem 2021 Mar 18;6(1):13. Epub 2021 Mar 18.

Peking University Cancer Hospital, Beijing, China.

Background: The Editorial Board of EJNMMI Radiopharmacy and Chemistry releases a biyearly highlight commentary to update the readership on trends in the field of radiopharmaceutical development.

Results: This commentary of highlights has resulted in 23 different topics selected by each member of the Editorial Board addressing a variety of aspects ranging from novel radiochemistry to first in man application of novel radiopharmaceuticals.

Conclusion: Trends in radiochemistry and radiopharmacy are highlighted demonstrating the progress in the research field being the scope of EJNMMI Radiopharmacy and Chemistry.
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http://dx.doi.org/10.1186/s41181-021-00128-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973323PMC
March 2021

Landscape scaling of different land-use types, geomorphological styles, vegetation regionalizations, and geographical zonings differs spatial erosion patterns in a large-scale ecological restoration watershed.

Environ Sci Pollut Res Int 2021 Mar 17. Epub 2021 Mar 17.

Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.

Water erosion is one of the main types of soil degradation, but few quantitative estimates have been done in the soil erosion intensity grades of different landscape scaling characteristics in a large-scale ecological restoration watershed. This study comparatively illustrates the utility of high-resolution data and geospatial technique, particularly the GIS-based RUSLE model, for an improved understanding of the spatial patterns of soil erosion under different land use types, geomorphological styles, vegetational regionalizations, and geographical zonings, aiming to promote ecological sustainable watershed management in practice. Results indicate the following:(i) The soil erosion intensity grade showed an overall decreasing trend from northwest to southeast in the Jinghe River Watershed. The percentages of intense erosion and extremely intense erosion in different land use types in 2015 were significantly lower than that in 2000 except middle- and low-coverage grasslands because of the effective implementation of "Grain for Green" project. e.g., the percentage of extremely intense erosion in dry land decreased from 41.72% in 2000 to 8.99% in 2015.(ii) The mid-elevation loessial beams and hills and mid-elevation loessial tableland were both the major sources of intense erosion and extremely intense erosion, with the contribution ratio of 41.89 and 53.94% and 35.79 and 20.83% in 2000 and 44.45 and 48.99% and 36.88 and 18.72% in 2015, respectively.(iii) The intense, extremely intense and severe erosion in the temperate typical grassland subzone accounted for 32.62, 44.47, and 9.6% in 2000 and 36.74, 40.7, and 8.1% in 2015, respectively. There were no severe and extremely intense erosion in the deciduous oak forest subzone of northern and southern warm temperate. (iv) The intense, extremely intense, and severe erosion of the loessial wide valley hills accounted for 32.19, 45.27, and 8.92% in 2000 and 36.32, 41.42, and 7.48% in 2015. The intense and extremely intense erosion of the loessial tableland accounted for 40.58 and 16.02% in 2000 and 40.57 and 14.27% in 2015. More importantly, land use types can be applied to the design and implementation of a development scheme more reliably than other landscapes because the land use system is a more scientific and fine classification based on topography, soils, and vegetation correlated with geology, geomorphology, and climate. The knowledge on the fine landscape scale will be useful for comprehensive watershed management by soil scientists, agronomists, environmental scientists, land managers, and policymakers.
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http://dx.doi.org/10.1007/s11356-021-13274-1DOI Listing
March 2021

Noninvasive evaluation of PD-L1 expression using Copper 64 labeled peptide WL12 by micro-PET imaging in Chinese hamster ovary cell tumor model.

Bioorg Med Chem Lett 2021 Mar 8;40:127901. Epub 2021 Mar 8.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), NMPA Key Laboratory for Research and Evaluation of Radiopharmaceuticals (National Medical Products Administration), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing 100142, China. Electronic address:

As an indicative biomarker for immunotherapy, PD-L1 plays an important role in the clinical decision-making of the immune checkpoint blockade therapy. PET imaging through radiotracer can real-timely, quantitatively, and non-invasively assess the expression of PD-L1 in tumors. Here, we reported a copper-64 radiolabeled NOTA-WL12, Cu-NOTA-WL12, and preliminarily evaluated its application in non-invasively detecting the PD-L1 expression.Cu-NOTA-WL12 was produced with high radiochemical yield (>90%), radiochemical purity (>98%), and specific activity (20 MBq/nmol). Cu-NOTA-WL12 showed high in vitro stability and high binding affinity to the PD-L1 (K ≈ 3.012 nM). The micro-positron emission tomography/computerized tomography (micro-PET/CT) imaging indicated that Cu-NOTA-WL12 was specifically accumulated in the tumor with PD-L1 expression. All results demonstrated that Cu-NOTA-WL12 holds great potential for noninvasive evaluation of PD-L1 expression levels.
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http://dx.doi.org/10.1016/j.bmcl.2021.127901DOI Listing
March 2021

Changes in plasma HDL and its subcomponents HDL2b and HDL3 regulate inflammatory response by modulating SOCS1 signaling to affect severity degree and prognosis of sepsis.

Infect Genet Evol 2021 Mar 5;91:104804. Epub 2021 Mar 5.

Department of Intensive Care Unit, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510180, China.

Objectives: To explore if SOCS1 is regulated by plasma HDL and its subcomponents HDL2b and HDL3 to affect inflammatory reaction then to influence the severity degree and prognosis of sepsis.

Methods: One hundred sepsis patients in ICU and 85 normal control persons from October 2018 to October 2019 in our hospital were enrolled. Adult male C57BL/6 mice were used to establish sepsis model by CLP method. HDL, CRP, and WBC count of human were measured using an auto-analyzer. Plasma HDL, IL-1β, and TNF-α proteins levels of mice were measured with ELISA. Microfluidic chip was used for plasma HDL2b and HDL3 detections. SOCS1 in liver and spleen of mice were measured by qRT-PCR. The relationship between plasma HDL//HDL2b and inflammatory indices/SOCS1 in liver/spleen was analyzed with spearman correlation coefficient method. The sepsis patients/mice were divided into non-survival and survival groups. The sepsis patients were divided into severe and mild sepsis patients based on the SOFA score or divided into high and low score groups according to the APACHE II score. The sepsis mice were divided into high and low score group based on the modified sepsis severity score criterion.

Results: Plasma HDL and HDL2b levels were significantly declined (P < 0.01), while HDL3 was normal in both sepsis patients and mice (P > 0.05). Plasma HDL and HDL2b were negatively associated with the serum CRP concentration and positively correlated with the prognosis and severity in sepsis patients (P < 0.05). Moreover, the downregulated plasma HDL but not HDL2b was negatively related to increased SOCS1 mRNA levels in liver and spleen of mice, which were positively connected with TNF-α and IL-1β protein levels (P < 0.05).

Conclusions: Plasma HDL is downregulated in sepsis, which may facilitate inflammatory reaction then activate the SOCS1 signaling to regulate the severity and affect prognosis of sepsis. The decline of plasma HDL2b content could aggravate the severity and poor prognosis of sepsis through facilitating inflammatory reaction. The plasma HDL3 is not involved in sepsis. The more and further explorations may be needed.
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http://dx.doi.org/10.1016/j.meegid.2021.104804DOI Listing
March 2021

Clinical efficacy of laparoscopic modified Parks operation on the ultra-low rectal cancer.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Feb;46(2):121-126

Department of Colorectal and Anal Surgery, Xiangya Hospital, Central South University, Changsha 410008.

Objectives: To investigate the clinical efficacy of laparoscopic modified Parks operation on the patients with ultra-low rectal cancer.

Methods: According to the preoperative stage and intraoperative anastomotic position, 98 patients with ultra-low rectal cancer above T2 stage underwent laparoscopic Dixon operation, modified Parks operation and Miles operation, respectively. All patients were divided into 3 groups: a Dixon operation group (=39), a modified Parks operation group (=43) and a Miles operation group (=16). The clinical data and postoperative follow-up results were collected, compared, and analyzed to evaluate the clinical efficacy of laparoscopic modified Parks operation.

Results: The patients were more obese, the distance between tumor and anal margin was closer, and the operation time was longer in the modified Parks operation group than those in the Dixon operation group (all <0.05). However, the results of bleeding volume, the number of lymph nodes and the postoperative complications in the modified park operation group were similar than those in the Dixon operation group (all >0.05). In addition, the exhaust and defecation time and the hospitalization time were shorter, and the total cost of hospitalization was lower in the modified Parks operation group than those in the Dixon operation group (all <0.05). Although the anal function in the modified Parks operation group was poor in the early postoperative period, it reached the same level as that in the Dixon operation group from 6 months after the treatments with the regular anal function exercise (>0.05). There were no significant differences in tumor size, body shape, distance between tumor and anal margin, operation time, bleeding volume, number of lymph nodes detected, postoperative exhaust and defecation time between the Miles operation group and the modified Parks operation group, but the hospitalization time was shorter, the incidence of complications and the total cost of hospitalization were lower in the modified Parks operation group (all <0.05).

Conclusions: Laparoscopic modified Parks operation is a safe, economical and effective anus preservation operation, which can not only save the anus for some patients who had to perform Miles operation, but also recover better and faster after operation. Although the early anal function of patients performed with the modified operation is poor, it can gradually recover to the same level as the patients performed with the Dixon operation.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.190774DOI Listing
February 2021

Lipoprotein glomerulopathy induced by ApoE Kyoto mutation in ApoE-deficient mice.

J Transl Med 2021 03 4;19(1):97. Epub 2021 Mar 4.

Department of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Sichuan, Guoxue Alley, 37#, Wuhou District, Chengdu, 610041, China.

Background: Lipoprotein glomerulopathy (LPG) is a rare autosomal dominant kidney disease that is most commonly caused by mutations in ApoE Kyoto (p.R43C) and ApoE Sendai (p.R163P). Differences in phenotype among the various ApoE mutations have been suggested, but the pathogenic role of ApoE Kyoto has not been validated in an animal model. This study intended to establish an ApoE Kyoto murine model and to further compare the pathologic differences between ApoE Kyoto and ApoE Sendai.

Method: Male ApoE-deficient mice, 3 months of age, were divided into five groups, including the AD-ApoE Sendai, AD-ApoE Kyoto, AD-ApoE3, AD-eGFP, and ApoE (-/-) groups. The first four groups received recombinant adenovirus that contained the entire coding regions of the human ApoE Sendai and ApoE Kyoto, apoE3, and eGFP genes, respectively. Fasting blood and urine samples were collected at multiple time points. Lipid profiles and urine albumin-creatinine ratio were measured. Renal and aortic histopathologic alterations were analyzed.

Results: After virus injection, plasma human ApoE was detected and rapidly reached the maximum level at 4-6 days in the AD-ApoE Kyoto and AD-ApoE Sendai groups (17.4 ± 3.1 µg/mL vs.: 22.2 ± 4.5 µg/mL, respectively) and at 2 days in the AD-ApoE3 group (38.4 µg/mL). The serum total cholesterol decreased by 63%, 65%, and 73% in the AD-ApoE Kyoto, AD-ApoE Sendai and AD-ApoE3 groups, respectively. There were no significant changes in serum triglyceride and urinary albumin-creatinine ratio among the five groups. Typical lipoprotein thrombi with positive ApoE staining were detected in the AD-ApoE Kyoto and AD-ApoE Sendai groups. The Oil-red O-positive glomerular area tended to be higher in the AD-ApoE Kyoto group (9.2%) than in the AD-ApoE Sendai (3.9%), AD-ApoE3 (4.8%), AD-eGFP (2.9%), and ApoE (-/-) (3.6%) groups. The atherosclerotic plaque area in the aorta was lower in the group injected with various ApoE mutations than in the group without injection of ApoE mutation.

Conclusions: In this animal study, we first established an ApoE Kyoto mutation murine model and confirmed its pathogenic role in LPG. Our results suggested that LPG may be more severe with the ApoE Kyoto than with the ApoE Sendai.
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http://dx.doi.org/10.1186/s12967-021-02765-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934380PMC
March 2021

Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers.

PLoS One 2021 4;16(3):e0247963. Epub 2021 Mar 4.

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, United States of America.

Current influenza vaccines do not elicit broadly protective immune responses against multiple strains. New strategies to focus the humoral immune response to conserved regions on influenza antigens are therefore required for recognition by broadly neutralizing antibodies. It has been suggested that B-cells with receptors that recognize conserved epitopes would be preferentially stimulated through avidity effects by mosaic particles presenting multiple forms of a variable antigen. We adapted SpyCatcher-based platforms, AP205 virus-like particles (VLPs) and mi3 nanoparticles (NPs), to covalently co-display SpyTagged hemagglutinin (HA) trimers from group 1 and group 2 influenza A strains. Here we show successful homotypic and heterotypic conjugation of up to 8 different HA trimers to both VLPs and NPs. We characterized the HA-VLPs and HA-NPs by cryo-electron tomography to derive the average number of conjugated HAs and their separation distances on particles, and compared immunizations of mosaic and homotypic particles in wild-type mice. Both types of HA particles elicited strong antibody responses, but the mosaic particles did not consistently elicit broader immune responses than mixtures of homotypic particles. We conclude that covalent attachment of HAs from currently-circulating influenza strains represents a viable alternative to current annual influenza vaccine strategies, but in the absence of further modifications, is unlikely to represent a method for making a universal influenza vaccine.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247963PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932532PMC
March 2021

Benign Adrenal Nodule Mimicking Lymphoma Differentially Diagnosed by 18F-FDG and 18F-FGln PET/CT.

Clin Nucl Med 2021 Mar 4. Epub 2021 Mar 4.

From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, China Department of Radiology, University of Pennsylvania, Philadelphia, PA Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China.

Abstract: 18F-FDG and 18F-(2S,4R)4-fluoroglutamine (18F-FGln) PET/CT were performed in a 58-year-old woman with suspected small intestinal malignancy for differential diagnosis and staging. 18F-FDG and 18F-FGln PET images showed consistent intense hypermetabolic lesion activity in part of the small intestine. Moreover, 18F-FDG/PET demonstrated a focal of increased radiotracer uptake in left adrenal lesion, which did not show abnormal activity on 18F-FGln/PET. The postoperative pathology confirmed that it was a peripheral T-cell lymphomas of the intestine, and the adrenal lesion was considered benign after continuous CT follow-up for more than 2 years.
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http://dx.doi.org/10.1097/RLU.0000000000003550DOI Listing
March 2021

Treatment of exostosin 1-associated membranous lupus nephritis with multiple low doses of rituximab: A case report.

Medicine (Baltimore) 2021 Mar;100(9):e24887

Renal Division, Department of Medicine.

Rationale: Membranous glomerulonephritis (MN) is the leading cause of nephrotic syndrome in adults and is classified as primary or secondary. Secondary MN accounts for 20% to 30% of all MN cases and can arise from a number of conditions, including autoimmune diseases. Recently exostosin 1/exostosin 2 (EXT1/EXT2) have been identified as the common antigens in secondary autoimmune MN and are present in cases of pure membranous lupus nephritis (LN). The treatment of EXT1/EXT2-associated MN remains elusive.

Patient Concerns: We present the case of a 15-year-old female who presented with nephrotic syndrome, positive ANA and dsDNA, and low serum complements. A renal biopsy revealed pure membranous nephritis with IgG and C3 deposition. EXT1 was found along the glomerular capillary walls and stained positive, while phospholipase A2 receptor (PLA2R) and thrombospondin type-1 domain-containing 7A (THSD7A) were negative.

Diagnosis: The patient was diagnosed with ETX1-associated membranous LN.

Interventions: She was treated with prednisone and multiple low-dose rituximab (4 200 mg doses, approximately every 2 months, based on CD19+ cells counts).

Outcomes: The patient had complete remission within 8 months later, and she remained in remission for the 16-month period of follow-up.

Lessons: To our knowledge, this is the first case of EXT1-associated MN that has been successfully treated by multiple low-dose rituximab. Further studies can investigate the optimal dosage and treatment protocol.
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http://dx.doi.org/10.1097/MD.0000000000024887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939150PMC
March 2021

Oxygen doping in antimony sulfide nanosheets to facilitate catalytic conversion of polysulfides for lithium-sulfur batteries.

Chem Commun (Camb) 2021 Apr 1;57(26):3255-3258. Epub 2021 Mar 1.

Key Laboratory of Carbon Materials of Zhejiang Province, Wenzhou University, Wenzhou 325035, P. R. China.

A high-performance catalyst, O-doped SbS nanosheets (SS-O NSs), is synthesized and introduced into lithium-sulfur batteries. Owing to their good conductivity, strong adsorbability/catalytic effect to polysulfides and fast Li diffusion, the SS-O NSs-modified cathodes can effectively mitigate the shuttle effect, thus achieving outstanding electrochemical performance.
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http://dx.doi.org/10.1039/d0cc08377aDOI Listing
April 2021

Effect of Activin A on activation status of monocytes in acute-phase Kawasaki disease.

Clin Exp Med 2021 Feb 25. Epub 2021 Feb 25.

Department of Rheumatology and Immunology, Shenzhen Children's Hospital, 7019 Yitian Road, Shenzhen, 518026, China.

Kawasaki disease is a kind of self-limited systemic vasculitis involving middle and small arteries, which usually occurs in children under 5 years old. Excessive inflammatory response caused by activation of monocytes is one of the important mechanisms of Kawasaki disease. Activated monocytes secrete large amounts of inflammatory mediators such as TNF-α and IL-1β. Activin A, a member of transforming growth factor-β superfamily, is a multifunctional growth and transforming factor. Several experimental evidences pinpoint that Activin A can regulate multiple biological function of the immune system. However, whether Activin A is involved in regulation of activation of monocytes in Kawasaki disease was not well characterized. Here, this study showed that the expression of Activin A in serum decreased in acute-phase Kawasaki disease. Furthermore, Activin A inhibits activin type IIA receptor, activin type IB receptor, CD86 and CD80 expression in over-activated monocytes. In addition, Activin A inhibited Smad3 expression and NF-κB signaling pathways. Specific function and mechanism of Activin A in acute-phase Kawasaki disease need further study.
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http://dx.doi.org/10.1007/s10238-021-00695-yDOI Listing
February 2021

Synthesis, preclinical evaluation, and a pilot clinical imaging study of [F]AlF-NOTA-JR11 for neuroendocrine neoplasms compared with [Ga]Ga-DOTA-TATE.

Eur J Nucl Med Mol Imaging 2021 Feb 25. Epub 2021 Feb 25.

Department of Nuclear Medicine, Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Peking University Cancer Hospital & Institute, No. 52 Fu-Cheng Rd., Beijing, 100142, China.

Purpose: A [F]AlF-labeled somatostatin receptor (SSTR) antagonist was developed for imaging of neuroendocrine neoplasms (NENs), evaluated and compared with [Ga]Ga-DOTA-TATE.

Method: [F]AlF-NOTA-JR11 was synthesized manually and qualified with high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). The cellular uptake, internalization, and saturation binding were performed with HEK293-SSTR2 cells. Biodistribution and micro-PET imaging were carried out with HEK293-SSTR2 tumor-bearing mice. [F]AlF-NOTA-JR11 PET/MR imaging and [Ga]Ga-DOTA-TATE PET/CT were performed with ten patients of NEN at 50~60 min post-injection (p.i.). Normal organ biodistribution and tumor detectability were evaluated.

Result: [F]AlF-NOTA-JR11(24~36 GBq/μmol) was prepared within 30 min and 51.35 ± 3.30% (n > 10)of radiochemical yield. The radiochemical purity was 98.74 ± 1.24% (n > 10). Two stereoisomers were found and confirmed by LC-MS. The cellular uptake of [F]AlF-NOTA-JR11 and [Ga]Ga-DOTA-TATE were 4.50 ± 0.31 and 4.50 ± 0.13 %AD/10 cells at 30 min, and the internalization at 37 °C of [F]AlF-NOTA-JR11 (5.47 ± 0.32% at 60 min) was significantly lower than [Ga]Ga-DOTA-TATE (66.89 ± 1.62% at 60 min). The affinity of [F]AlF-NOTA-JR11 (K = 11.59 ± 1.31 nM) was slightly lower than [Ga]Ga-DOTA-TATE (K = 7.36 ± 1.02 nM); [F]AlF-NOTA-JR11 showed high uptake in tumor (9.02 ± 0.92 %ID/g at 60 min p.i.) which can be blocked by 50 μg of NOTA-JR11 (3.40 ± 1.64 %ID/g at 60 min p.i.); the result was coincident with micro-PET imaging. Imaging study of NEN patients showed that more lesions were found only by [F]AlF-NOTA-JR11 (n = 67 vs. 1 only by [Ga]Ga-DOTA-TATE), and the uptakes of [F]AlF-NOTA-JR11 in majority normal organs were significantly lower than [Ga]Ga-DOTA-TATE. The target to nontarget of maximum of standard uptake value (SUVmax) of [F]AlF-NOTA-JR11 in liver lesions were significantly higher than those of [Ga]Ga-DOTA-TATE.

Conclusion: Qualitied [F]AlF-NOTA-JR11 is prepared conveniently with reasonable yield, and it can bind SSTR2 specifically with high affinity. Excellent imaging capability of [F]AlF-NOTA-JR11 for NENs is superior to [Ga]Ga-DOTA-TATE, especially in digestive system. It has a great potential for imaging of NENs.
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http://dx.doi.org/10.1007/s00259-021-05249-8DOI Listing
February 2021

The effect of Q-switched 1064-nm Nd: YAG laser on skin barrier and collagen synthesis via miR-663a to regulate TGFβ1/smad3/p38MAPK pathway.

Photodermatol Photoimmunol Photomed 2021 Feb 23. Epub 2021 Feb 23.

Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.

Background: Our previous research found that Q-switched 1064-nm Nd: YAG laser (1064-QSNYL) induces skin collagen synthesis by activating TGFβ1/Smad3/p38MAPKs pathway. Moreover, a lot of studies shown that MicroRNAs (miRNAs) contribute to regulate collagen synthesis and skin barrier. Therefore, we intend to explore the mechanism of 1064-QSNYL on collagen synthesis and skin barrier through miRNAs.

Methods: We predicted the upstream miRNAs of TGFβ1 by bioinformatics databases, and verified them through dual-luciferase reporter genes and Western blotting. The expression of collagen, skin barrier-related protein K10 and filaggrin, TIMP-1, and MMP-2 were detected by RT-qPCR and Western blotting, respectively. Moreover, we detected moisture content, elasticity value, TEWL value, SOD vitality, and hydroxyproline content to evaluate skin barrier of mice. H&E staining to observe the change of dermis thickness and inflammation and infiltration of mice skin.

Results: The results shown that TGFβ1 was target gene of miR-663a. Moreover, we found that 1064-QSNYL activated TGFβ1/smad3/p38MAPK pathway by down-regulating the expression of miR-663a in HaCaT, HDF cells, and mice, thereby promoting expression of Collagen I, Collagen IV, TIMP-1, K10, and filaggrin and inhibiting MMP-2. Furthermore, 1064-QSNYL contributed to moisture content, elasticity, SOD vitality, and hydroxyproline content via miR-663a to activate TGFβ1/smad3/p38MAPK pathway.

Conclusions: In summary, this study found for the first time that 1064-QSNYL contributed to collagen synthesis and skin repair via miR-663a to regulate TGFβ1/smad3/p38MAPK pathway, thereby achieving skin rejuvenation.
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http://dx.doi.org/10.1111/phpp.12673DOI Listing
February 2021

The effect of TMJ disk repositioning by suturing through open incision on adolescent mandibular asymmetry with and without a functional orthodontic appliance.

Oral Surg Oral Med Oral Pathol Oral Radiol 2021 Apr 8;131(4):405-414. Epub 2020 Dec 8.

Resident, Department of Oral and Cranio-maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, Huang Pu District, Shanghai, China.

Objective: The objective of this study was to evaluate the effect of combined temporomandibular joint (TMJ) disk repositioning by suturing through open incision and orthodontic functional appliance (OFA) treatment for adolescents with mandibular asymmetry.

Study Design: Adolescent patients (12-20 years old) with mandibular asymmetry combined with unilateral TMJ disk displacement without reduction were treated with disk repositioning by suturing through open incision with and without postoperative OFA. Magnetic resonance imaging and posteroanterior cephalometric radiographs (PA) were used to measure and compare the changes in condylar height, joint space, and menton deviation pre- and postoperatively.

Results: Twenty-six patients were included in the study. Joint space was significantly increased postoperatively and new bone mostly formed at the superior or posterior superior part of the condyle after 6 to 18 months in all surgically treated joints. Fourteen patients with OFA had a significant increase in condylar height and menton deviation compared to 12 patients without OFA (2.29 ± 0.91 mm vs 1.22 ± 0.69 mm, P = .003; 4.56 ± 1.48 mm vs 2.01 ± 0.74 mm, P = .000).

Conclusions: Combined treatment with TMJ disk repositioning by suturing through open incision and OFA can promote condylar growth and correct mandibular deviation in adolescent patients. Postoperative OFA can maintain the increased joint space created by disk repositioning and promote new bone formation at the superior and posterior parts of the condyle.
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http://dx.doi.org/10.1016/j.oooo.2020.11.014DOI Listing
April 2021

A versatile approach for shape-controlled synthesis of ultrathin perovskite nanostructures.

Dalton Trans 2021 Mar;50(9):3308-3314

Centre for Micro and Nano Devices, Department of Physics, COMSATS Institute of Information Technology, Islamabad, 44500, Pakistan.

Very recently, ultrathin perovskite nanostructures, with the advantages of perovskite and ultrathin properties, have received an enormous level of interest due to their many fascinating properties, such as a strong quantum confinement effect and a large specific surface area. In spite of this incredible success of perovskite nanocrystals (NCs), the development of perovskite NCs is still in its infancy, and the production of high-quality ultrathin perovskite nanostructures has been a hot topic in the fields of nanoscience and nanotechnology. Herein, we demonstrate that ultrathin CsPbBr3 perovskite nanosheets (NSs) can be obtained by a simple mixing of precursor-ligand complexes under ambient conditions. It was found that the formation of NSs is ascribed to the stepwise self-assembly of the initially formed different types of ultrathin nanostructures. Due to the disappearance of grain boundaries and protection of branched ligands, these NSs exhibit enhanced optical properties compared to other types of samples. This direct synthesis method opens up a promising road for the synthesis of ultrathin NSs and guides the fabrication of other ultrathin nanostructures.
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http://dx.doi.org/10.1039/d0dt04203jDOI Listing
March 2021

F-Boramino acid PET/CT in healthy volunteers and glioma patients.

Eur J Nucl Med Mol Imaging 2021 Feb 15. Epub 2021 Feb 15.

Radiochemistry and Radiation Chemistry Key Laboratory of Fundamental Science, Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.

Purpose: In this work, the safety, biodistribution, and radiation dosimetry of large neutral amino acid transporter type-1 (LAT-1) targeting PET tracer F-trifluorobborate-derived tyrosine (denoted as F-FBY) has been investigated. It is designed as a first-in-human study in healthy volunteers and to assay LAT-1 expression level in glioma patients.

Methods: Six healthy volunteers (3 M, 3 F) underwent whole-body PET acquisitions at multiple time points after bolus injection of F-FBY. Regions of interest (ROIs) were mapped manually on major organs, and then the time-activity curves (TACs) were obtained. Dosimetry was calculated with the OLINDA/EXM software. Thirteen patients who were suspected of glioma were scanned with PET/CT at 30 min after F-FBY injection. Within 7 days after PET/CT, the tumor was removed surgically, and LAT-1 immunohistochemical staining for LAT-1 was performed on tumor samples and correlated with F-FBY PET imaging.

Results: F-FBY was well tolerated by all healthy volunteers, and no adverse symptoms were observed or reported. F-FBY is rapidly cleared from the blood circulation and excreted mainly through the kidneys and urinary tract. The effective dose (ED) was 0.0039 ± 0.0006 mSv/MBq. In 14 surgical confirmed gliomas (one of the patiens had two gliomas), F-FBY uptake increased consistently with tumor grade, with maximum standard uptake values (SUV) of 0.28 ± 0.14 and 2.84 ± 0.46 and tumor-to-normal contralateral activity (T/N) ratio of 2.30 ± 1.26 and 24.56 ± 6.32 in low- and high-grade tumors, respectively. In addition to the significant difference in the uptakes between low- and high-grade gliomas (P < 0.001), the immunohistochemical staining confirmed the positive correlations between the SUV, LAT-1 expression (r = 0.80, P < 0.001), and Ki-67 labeling index (r = 0.79, P < 0.001).

Conclusion: F-FBY is a PET tracer with favorable dosimetry profile and pharmacokinetics. It has the potential to assay LAT-1 expression in glioma patients and may provide imaging guidance for further boron neutron capture therapy of gliomas.

Trial Registration: clinicaltrials.gov (NCT03980431).
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http://dx.doi.org/10.1007/s00259-021-05212-7DOI Listing
February 2021

mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants.

Nature 2021 Feb 10. Epub 2021 Feb 10.

Laboratory of Molecular Immunology, The Rockefeller University, New York, NY, USA.

Here we report on the antibody and memory B cell responses of a cohort of 20 volunteers who received the Moderna (mRNA-1273) or Pfizer-BioNTech (BNT162b2) vaccine against SARS-CoV-2. Eight weeks after the second injection of vaccine, volunteers showed high levels of IgM and IgG anti-SARS-CoV-2 spike protein (S) and receptor-binding-domain (RBD) binding titre. Moreover, the plasma neutralizing activity and relative numbers of RBD-specific memory B cells of vaccinated volunteers were equivalent to those of individuals who had recovered from natural infection. However, activity against SARS-CoV-2 variants that encode E484K-, N501Y- or K417N/E484K/N501-mutant S was reduced by a small-but significant-margin. The monoclonal antibodies elicited by the vaccines potently neutralize SARS-CoV-2, and target a number of different RBD epitopes in common with monoclonal antibodies isolated from infected donors. However, neutralization by 14 of the 17 most-potent monoclonal antibodies that we tested was reduced or abolished by the K417N, E484K or N501Y mutation. Notably, these mutations were selected when we cultured recombinant vesicular stomatitis virus expressing SARS-CoV-2 S in the presence of the monoclonal antibodies elicited by the vaccines. Together, these results suggest that the monoclonal antibodies in clinical use should be tested against newly arising variants, and that mRNA vaccines may need to be updated periodically to avoid a potential loss of clinical efficacy.
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http://dx.doi.org/10.1038/s41586-021-03324-6DOI Listing
February 2021