Publications by authors named "Zhi Wang"

1,928 Publications

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Differences in neuroanatomy and functional connectivity between motor subtypes of Parkinson's disease.

Front Neurosci 2022 22;16:905709. Epub 2022 Jul 22.

Department of Neurology, Henan Provincial People's Hospital, Zhengzhou, China.

Background: The "postural instability/gait difficulty" (PIGD) and "tremor-dominant" (TD) motor subtypes of Parkinson's disease (PD) differ in their clinical manifestations. The neurological basis of these differences is unclear.

Methods: We performed voxel-based morphometric analysis and measured amplitudes of low-frequency fluctuation (ALFF) on 87 PIGD patients and 51 TD patients. We complemented this neuroanatomical comparison with seed-to-voxel analysis to explore differences in functional connectivity.

Results: The PIGD group showed significantly smaller gray matter volume in the medial frontal gyrus (mainly on the right side) than the TD group. Across all patients, gray matter volume in the medial frontal gyrus correlated negatively with severity of PIGD symptoms after controlling for age ( = -0.250, = 0.003), but this correlation was not observed in separate analyses of only PIGD or TD patients. The PIGD group showed greater functional connectivity of the right superior frontal gyrus with the left lingual gyrus, right lateral occipital cortex, and right lingual gyrus. ALFF did not differ significantly between the two groups.

Conclusion: Postural instability/gait difficulty may be associated with smaller gray matter volume in medial frontal gyrus than TD, as well as with greater functional connectivity between the right superior frontal gyrus and occipital cortex. These results may help explain the clinical differences between the two motor subtypes of PD.
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http://dx.doi.org/10.3389/fnins.2022.905709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354573PMC
July 2022

Altered Brain Function in Treatment-Resistant and Non-treatment-resistant Depression Patients: A Resting-State Functional Magnetic Resonance Imaging Study.

Front Psychiatry 2022 22;13:904139. Epub 2022 Jul 22.

Department of Psychiatric, Beijing First Hospital of Integrated Chinese and Western Medicine, Beijing, China.

Objective: In this study, we used amplitude of low-frequency fluctuation (ALFF) and regional homogeneity (ReHo) to observe differences in local brain functional activity and its characteristics in patients with treatment-resistant depression (TRD) and non-treatment-resistant depression (nTRD), and to explore the correlation between areas of abnormal brain functional activity and clinical symptoms.

Method: Thirty-seven patients with TRD, 36 patients with nTRD, and 35 healthy controls (HCs) were included in resting-state fMRI scans. ALFF and ReHo were used for image analysis and further correlation between abnormal brain regions and clinical symptoms were analyzed.

Results: ANOVA revealed that the significantly different brain regions of ALFF and ReHo among the three groups were mainly concentrated in the frontal and temporal lobes. Compared with the nTRD group, the TRD group had decreased ALFF in the left/right inferior frontal triangular gyrus, left middle temporal gyrus, left cuneus and bilateral posterior lobes of the cerebellum, and increased ALFF in the left middle frontal gyrus and right superior temporal gyrus, and the TRD group had decreased ReHo in the left/right inferior frontal triangular gyrus, left middle temporal gyrus, and increased ReHo in the right superior frontal gyrus. Compared with the HC group, the TRD group had decreased ALFF/ReHo in both the right inferior frontal triangular gyrus and the left middle temporal gyrus. Pearson correlation analysis showed that both ALFF and ReHo values in these abnormal brain regions were positively correlated with HAMD-17 scores ( < 0.05).

Conclusion: Although the clinical symptoms were similar in the TRD and nTRD groups, abnormal neurological functional activity were present in some of the same brain regions. Compared with the nTRD group, ALFF and ReHo showed a wider range of brain area alterations and more complex neuropathological mechanisms in the TRD group, especially in the inferior frontal triangular gyrus of the frontal lobe and the middle temporal gyrus of the temporal lobe.
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http://dx.doi.org/10.3389/fpsyt.2022.904139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352890PMC
July 2022

A core-shell structured magnetic sulfonated covalent organic framework for the extraction of benzoylureas insecticides from water, pear juice and honey samples.

J Chromatogr A 2022 Jul 30;1679:463387. Epub 2022 Jul 30.

Department of Chemistry, College of Science, Hebei Agricultural University, Baoding 071001, China; Department of Food Science, College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China. Electronic address:

Three magnetic covalent organic frameworks (named M-TpPa-SONa, M-TpPa-SOH and M-TpPa) were prepared by the solvothermal synthesis method with 1,3,5-trimethylphenol (TP) and either 2-sulfo-1,4-phenylenediamine (Pa-SOH) or p-phenylenediamine (Pa) as monomers. Among them, the M-TpPa-SONa possessed relatively high hydrophilicity, good magnetic responsiveness, and high affinity for the benzoylureas (BUs) insecticides. It was then explored as the magnetic solid-phase extraction adsorbent for the extraction of six BUs (diflubenzuron, triflumuron, hexaflumuron, teflubenzuron, flufenoxuron and chlorfluazuron) from water, pear juice and honey samples prior to high-performance liquid chromatography with ultraviolet detection. Under the optimized experimental conditions, a good linearity was achieved within the concentration range of 0.27-40.0 ng mL for water sample, 0.47-30.0 ng mL for pear juice sample, and 2.70-200.0 ng g for honey sample. The limits of detection for the analytes were 0.08-0.11 ng mL for water sample, 0.14-0.19 ng mL for pear juice sample and 0.80-1.00 ng g for honey sample. The method recoveries for spiked samples were in the range of 85.0%-111.0% with the relative standard deviations less than 8.8%. The developed method was successfully used for the determination of the BUs in water, pear juice and honey samples.
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http://dx.doi.org/10.1016/j.chroma.2022.463387DOI Listing
July 2022

Study on the Relationship between Lung Cancer Stromal Cells and Air Cavity Diffusion Based on an Image Acquisition System.

Contrast Media Mol Imaging 2022 14;2022:2492124. Epub 2022 Jul 14.

Department of Emergency, The Hospital of Xidian Group, No. 97 FengDeng Rd, Xian, China.

Objective: The study aimed to investigate the role of tumor stromal cells in the pathogenesis of STAS, the relationship between air diffusion (STAS) and tumor stromal cells (TSCs) was studied, and the prognostic significance of TSC and STAS in patients with lung adenocarcinoma was evaluated.

Methods: A total of 150 patients with lung cancer diagnosed in the Affiliated Hospital of Jiangsu Province were selected. From the perspective of pathology, medical information technology was used to assist the diagnosis. The data of multiple magnetic resonance images were analyzed by three-dimensional space convolution (CNN), fuzzy neural network (FNN), transfinite learning machine (ELM), and binarization.

Result: After data fusion, the specificity and sensitivity of multiple magnetic resonance (MRI) data are significantly higher than those of single MRI data, and the more fusion times, the better the sensitivity and specificity. With the increase in the number of information and data fusion, the proportion of the significant effect and the comprehensive effective rate of patients are on the rise. Multiple MRI data fusion examination and analysis under medical information technology can improve the cure rate of patients, and the 1-year survival rate and the 3-year survival rate of patients have also gradually improved.

Conclusion: The MRI data fusion diagnosis method under the application of information technology can improve the sensitivity and specificity of the diagnosis results and comprehensively improve the clinical cure rate and the survival rate at different times of prognosis. In the context of the current big data information age, this multifeature fusion analysis technology is playing a more and more important role in medical treatment. The application of this method and technology not only improves the quality of life of patients but also processes multiple types of data at one time only by using the proposed medical assistant diagnosis model, which can save the diagnosis time to a certain extent. It has effectively realized the medical management and medical service quality and has important promotion significance.
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http://dx.doi.org/10.1155/2022/2492124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303510PMC
August 2022

A classical [VO] anion templated high-nuclearity silver thiolate cluster.

Chem Commun (Camb) 2022 Jul 28. Epub 2022 Jul 28.

School of Chemistry and Chemical Engineering, State Key Laboratory of Crystal Materials, Shandong University, Ji'nan, 250100, People's Republic of China.

Polyoxovanadates (POVs) as templates are still scarcely observed in silver clusters. Herein, the largest known POV-based silver cluster (Ag50) was synthesized, which is a core-shell conformation composed of the generated classical [VO] core and Ag shell, constrained by the S- and O-donor ligands with a specific distribution. Such {[email protected]} structure displays geometric inheritance from the symmetric decavanadate to the silver skeleton. The solution behavior, solid-state stability and photoelectric properties are discussed in detail. This work provides enlightenment for the further construction of POV-templated high-nuclearity silver clusters.
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http://dx.doi.org/10.1039/d2cc03003aDOI Listing
July 2022

Effect of sodium-glucose cotransporter-2 inhibitors on blood pressure in patients with heart failure: a systematic review and meta-analysis.

Cardiovasc Diabetol 2022 Jul 25;21(1):139. Epub 2022 Jul 25.

Department of Cardiovascular Disease, Peking University First Hospital, Beijing, China.

Background: Recent studies have shown that sodium-glucose cotransporter-2 inhibitors (SGLT2i) can achieve significant improvement in blood pressure in people with diabetes. Furthermore, randomized controlled trials (RCTs) have established that SGLT2i have a cardioprotective effect in adults with heart failure (HF). Therefore, we performed this systematic review an meta-analysis to determine the effect of SGLT2i on blood pressure in patients with HF.

Methods: We used the Medline, Cochrane Library, Embase, and PubMed databases to identify RCTs (published through to April 29, 2022) that evaluated the effect of SGLT2i on HF. The primary endpoint was defined as change in blood pressure. Secondary composite outcomes were heart rate, hematocrit, body weight, and glycated hemoglobin. The N-terminal pro-brain natriuretic peptide level, Kansas City Cardiomyopathy Questionnaire scores, and estimated glomerular filtration rate were also evaluated.

Results: After a literature search and detailed evaluation, 16 RCTs were included in the quantitative analysis. Pooled analyses showed that SGLT2i were associated with a statistically significant reduction in systolic blood pressure of 1.68 mmHg (95% confidence interval [CI] - 2.7, - 0.66; P = 0.001; I = 45%) but not diastolic blood pressure (mean difference [MD] -1.06 mmHg; 95% CI -3.20, 1.08; P = 0.33; I = 43%) in comparison with controls. Furthermore, SGLT2i decreased body weight (MD - 1.36 kg, 95% CI - 1.68, - 1.03; P < 0.001; I = 61%) and the glycated hemoglobin level (MD - 0.16%, 95% CI - 0.28, -0.04, P = 0.007; I = 91%) but increased hematocrit (MD 1.63%, 95% CI 0.63, 2.62, P = 0.001; I = 100%). There was no significant between-group difference in heart rate (MD - 0.35; 95% CI - 2.05, 1.35, P = 0.69; I = 0).

Conclusions: SGLT2i decreased systolic blood pressure in patients with HF but had no effect on diastolic blood pressure. These inhibitors may have numerous potentially beneficial clinical effects in patients with HF.
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http://dx.doi.org/10.1186/s12933-022-01574-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317067PMC
July 2022

Imaging of Endogenous Hydrogen Peroxide Efflux from Living Cells Bipolar Gold Nanoelectrode Array and Electrochemiluminescence Technology.

ACS Sens 2022 Jul 25. Epub 2022 Jul 25.

Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, Jiangsu Provincial Key Laboratory of Critical Care Medicine, Key Laboratory of Environmental Medicine Engineering, Ministry of Education, State Key Laboratory of Bioelectronics, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.

The integration of a closed bipolar electrode (-BPE) array and electrochemiluminescence (ECL) detection received a boost in applications in the detection of cell adhesion and disease-related biomarkers. This work proposed a gold nanorod array based -BPE-ECL system to realize an image of endogenous hydrogen peroxide (HO) efflux from living cells and parallel analysis of endogenous HO released from multiple cells by converting electrochemical signals into optical signals. The gold nanorod array with high density was prepared by a repeating chronopotentiometry procedure with anodic aluminum oxide (AAO) membrane as a template. The -BPE array was fabricated by assembling poly(dimethylsiloxane) (PDMS) chips on both sides of the gold nanorod array. When an appropriate driving potential is applied, HO generated from living cells at the sensing pole was reduced on the gold nanorod, triggering the oxidation of the ECL reagent at the reporting pole, which allowed the detection of HO released from living cells. Under phorbol myristate acetate (PMA) stimulation, HO released from living HeLa, HepG2, MCF-7, and LO2 cells was determined to be 47, 32.4, 25.7, and 6.3 μM, respectively. This indicated that the amount of HO released from PMA-stimulated cancer cells was significantly higher than that from the stimulated normal cells. This work presented a new approach for imaging of HO released from living cells and could also be used to detect other electrochemically active or non-electrochemically active molecules through simple cell surface modification, which may have potential applications in cell apoptosis study and disease diagnosis.
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http://dx.doi.org/10.1021/acssensors.2c01195DOI Listing
July 2022

Sensitive determination of auxins in environmental water and peach beverage by hyper crosslinked polymer-based solid-phase extraction with high performance liquid chromatography-fluorescence detection.

J Chromatogr A 2022 Jul 14;1678:463345. Epub 2022 Jul 14.

Department of Chemistry, College of Science, Hebei Agricultural University, Baoding, Hebei 071001, China; Department of Food Science, College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China. Electronic address:

As plant regulators, auxins can promote plant growth. However, they have toxicity and may cause harm to humans. Due to their low concentrations in food sample matrices, the enrichment and analysis of trace auxins in food samples is a challenging work. In this work, a series of hyper crosslinked polymers (HCPs) were synthesized by Friedel-Crafts acylation to extract four auxins (indole-3-acetic acid, indole-3-propionic acid, indole-3-butyric acid and 1-naphthylacetic acid). Among these HCPs, the QP-TC-HCP, synthesized from p-quaterphenyl (QP) and terephthaloyl chloride (TC), showed the best adsorption performance for the auxins. It was then applied as the adsorbent for the solid-phase extraction of the auxins from environmental water and peach beverage samples, followed by high performance liquid chromatography-fluorescence detection. Under the optimized conditions, the limits of detection were 3.0-12.0 pg mL for environmental water and 18.0-36.0 pg mL for peach beverage sample. The method recoveries of the auxins for the spiked samples were in the range of 85.0-110.0%. The established method provided an alternative approach for the determination of auxins in food samples. In addition, different types of organic compounds were tested for the extraction by the QP-TC-HCP to assess its application potential and adsorption mechanism. It was concluded that the QP-TC-HCP had better extraction performance for the compounds with certain hydrophilicity and more hydrogen bonding sites.
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http://dx.doi.org/10.1016/j.chroma.2022.463345DOI Listing
July 2022

Unifying the order and disorder dynamics in photoexcited VO.

Proc Natl Acad Sci U S A 2022 Jul 6;119(28):e2122534119. Epub 2022 Jul 6.

Materials Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720.

Photoinduced phase transition (PIPT) is always treated as a coherent process, but ultrafast disordering in PIPT is observed in recent experiments. Utilizing the real-time time-dependent density functional theory method, here we track the motion of individual vanadium (V) ions during PIPT in VO and uncover that their coherent or disordered dynamics can be manipulated by tuning the laser fluence. We find that the photoexcited holes generate a force on each V-V dimer to drive their collective coherent motion, in competing with the thermal-induced vibrations. If the laser fluence is so weak that the photoexcited hole density is too low to drive the phase transition alone, the PIPT is a disordered process due to the interference of thermal phonons. We also reveal that the photoexcited holes populated by the V-V dimerized bonding states will become saturated if the laser fluence is too strong, limiting the timescale of photoinduced phase transition.
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http://dx.doi.org/10.1073/pnas.2122534119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9282394PMC
July 2022

Integrative analysis uncovers response mechanism of Pirata subpiraticus to chronic cadmium stress.

Environ Sci Pollut Res Int 2022 Jul 21. Epub 2022 Jul 21.

College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China.

Soil cadmium (Cd) pollution is global environmental pollution and adversely affects paddy field organisms. Wolf spider grants a new insight to evaluate the toxicity triggered by Cd, yet the impact of chronic Cd exposure on the spider and its molecular mechanism remains unclear. The present study found that the wolf spider Pirata subpiraticus fed with Cd-accumulated flies for 5 weeks presented lower catalase, peroxidase, and acetylcholinesterase activities and higher malonaldehyde content than the control spiders (p < 0.05). An in-depth transcriptomic analysis yielded a total of 5995 differentially expressed genes (DEGs, with 3857 up-regulated and 2138 down-regulated genes) from the comparison, and 19 DEGs encoding three enzymatic indicators were down-regulated. Further enrichment analysis indicated that Cd stress could inhibit the expression of cuticle and chitin-encoding genes via the down-regulation of several key enzymes, such as chitin synthase, glutamine-fructose-6-phosphate transaminase, and chitinase. In addition, our findings suggested that hedgehog and FoxO signaling pathways might play an essential role in regulating survival, cell cycle, and autophagy process in spiders, which were primarily down-regulated under Cd stress. An intensely interactive network displayed that Cd exposure could repress key biological processes in P. subpiraticus, particularly peptide metabolic process and peptide biosynthetic process. To sum up, this integrative investigation confirmed an effective bioindicator for assessing Cd-induced toxicity; provided a mass of genes, proteins, and enzymes for further validation; and granted novel perspectives to uncover the molecular responses of spiders to Cd pollution.
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http://dx.doi.org/10.1007/s11356-022-22043-7DOI Listing
July 2022

Synthesis of nitrogen-rich magnetic hypercrosslinked polymer as robust adsorbent for the detection of neonicotinoids in honey, tomatoes, lettuce and Chinese cabbage.

J Chromatogr A 2022 Aug 9;1677:463326. Epub 2022 Jul 9.

College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China; College of Science, Hebei Agricultural University, Baoding 071001, China. Electronic address:

In this study, a novel nitrogen-rich magnetic hypercrosslinked polymer ([email protected]) was fabricated via a facile one-pot reaction of 1,3,5-triphenylbenzene, 2,4-dichloropyrimidine and phenyl-functionalized magnetic FeO nanoparticles. The [email protected] possessed high specific surface, abundant nanopores and good stability. Especially, [email protected] exhibited excellent adsorption capability with fast adsorption rate to neonicotinoid insecticides. It was applied as a superior magnetic adsorbent for rapid enrichment and isolation of four neonicotinoid insecticides (thiamethoxam, imidacloprid, acetamiprid and thiacloprid) from honey, tomato, lettuce and Chinese cabbage samples prior to high performance liquid chromatography-diode array detection. Under the optimized conditions, the good linearity was obtained in the range of 1-1000 ng g for honey, tomato, lettuce and Chinese cabbage samples, and the detection limits (S/N = 3) were 0.30-0.67 ng g for honey, 1-1.5 ng g for tomato, lettuce and Chinese cabbage. The spiked recoveries were 80.1-111%, and relative standard deviations were less than 10.0%. These results demonstrate that the developed method can be used as a good alternative method for sensitive determination of neonicotinoid insecticides in complex samples.
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http://dx.doi.org/10.1016/j.chroma.2022.463326DOI Listing
August 2022

Controlling spring Dinoflagellate blooms in a stratified drinking water reservoir via artificial mixing: Effects, mechanisms, and operational thresholds.

Sci Total Environ 2022 Jul 16;847:157400. Epub 2022 Jul 16.

Lijiahe Reservoir management Co., Ltd., Xi'an 710016, China.

Water-lifting aerators (WLAs) are often applied in stratified reservoirs to activate artificial mixing to inhibit harmful algal blooms (HABs). Here, the effects, mechanisms, and operational thresholds of spring Dinoflagellate control via artificial mixing were studied using a combination of taxonomic and functional groups and boundary line models. Algal cell density at two sampling sites (i.e., S1 and S2) decreased to below 1.0 × 10 cells/L (corresponding chlorophyll-a content under 10 μg/L) during artificial mixing, with a Dinoflagellate removal efficiency of 97.1 % at S1 and 95.5 % at S2, respectively. The succession patterns of main phytoplankton taxonomic and functional groups changed greatly during artificial mixing at the sites: from Dinoflagellate and motile Chlorophyta to Bacillariophyta from groups A/L/P to A, respectively. Water temperature (WT), light availability (Z/Z), and mixing depth (Z) were more effective factors influencing phytoplankton dynamics at a short-term scale, followed by total phosphorus (TP). A decrease in surface WT and Z/Z, and increase in Z alongside the improvement of TP levels, which were induced by WLAs, drove the Dinoflagellate bloom control by a shift of phytoplankton structure from large, motile, and low surface to volume ratio (S/V) to small, immobile, and high S/V algae. The operational threshold values of WT, Z/Z, Z and TP concentration for the suppression of Dinoflagellate growth using mixing systems are recommended as 9.6 °C, 0.17, 11.5 m, and 0.020 mg/L, respectively, based on a boundary line analysis. This work can help improve the cognition of mechanisms controlling HABs using mixing and aeration techniques in reservoirs.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157400DOI Listing
July 2022

Microcystin pollution in lakes and reservoirs: A nationwide meta-analysis and assessment in China.

Environ Pollut 2022 Jul 15;309:119791. Epub 2022 Jul 15.

Key Laboratory for Environment and Disaster Monitoring and Evaluation of Hubei, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430077, China. Electronic address:

The frequent occurrence of microcystins (MCs) has caused a series of water security issues worldwide. Although MC pollution in natural waters of China has been reported, a systematic analysis of the risk of MCs in Chinese lakes and reservoirs is still lacking. In this study, the distribution, trend, and risk of MCs in Chinese lakes and reservoirs were comprehensively revealed through meta-analysis for the first time. The results showed that MC pollution occurrence in numerous lakes and reservoirs have been reported, with MC pollution being distributed in the waters of 15 provinces in China. For lakes, the maximum mean total MC (TMC) and dissolved MC (DMC) concentrations occurred in Lake Dianchi (23.06 μg/L) and Lake Taihu (1.00 μg/L), respectively. For reservoirs, the maximum mean TMC and DMC concentrations were detected in Guanting (4.31 μg/L) and Yanghe reservoirs (0.98 μg/L), respectively. The TMC concentrations in lakes were significantly higher than those in the reservoirs (p < 0.05), but no difference was observed in the DMC between the two water bodies (p > 0.05). Correlation analysis showed that the total phosphorus concentrations, pH, transparency, chlorophyll a, and dissolved oxygen were significantly related to the DMC in lakes and reservoirs. The ecological risks of DMC in Chinese lakes and reservoirs were generally at low levels, but high or moderate ecological risks of TMC had occurred in several waters, which were not negligible. Direct drinking water and consumption of aquatic products in several MC-polluted lakes and reservoirs may pose human health risks. This study systematically analyzed the pollution and risk of MCs in lakes and reservoirs nationwide in China and pointed out the need for further MC research and management in waters.
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http://dx.doi.org/10.1016/j.envpol.2022.119791DOI Listing
July 2022

B16F10 Cell Membrane-Based Nanovesicles for Melanoma Therapy Are Superior to Hyaluronic Acid-Modified Nanocarriers.

Mol Pharm 2022 08 18;19(8):2840-2853. Epub 2022 Jul 18.

Department of Pharmaceutical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Some cancer cell membrane (CCM)-derived nanovesicles show strong homing effects and are used for targeted cancer therapy. By co-constructing the B16F10 cell membrane with a PEGylated phospholipid membrane, a new nanocarrier with a composite nanocrown structure was developed, which can evade immune recognition and actively target homologous melanoma. The nanocrowns have an encapsulation efficiency of more than 90% for paclitaxel and showed no significant difference ( > 0.05) from the PEGylated phospholipid membrane vesicles. Compared with the hyaluronic acid-modified PEGylated phospholipid membrane vesicles, the biomimetic nanocrowns enhanced the escape of nanovesicles from reticuloendothelial cells in vitro and extended the circulation time in vivo; moreover, the nanocrowns showed superior melanoma-targeted drug delivery capability and improved anticancer effects of paclitaxel as demonstrated by the inhibition of B16F10 cell proliferation and induction of apoptosis by interfering with microtubule formation. In contrast, the modification of hyaluronic acid did not increase the targeting capacity or antitumor effects of the nanocrowns, confirming that the superior targeting capacity was mediated by the exposed homologous CCMs rather than by hyaluronic acid. Our results demonstrate the potential of using biomimetic nanocrowns for active melanoma-targeted therapy.
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http://dx.doi.org/10.1021/acs.molpharmaceut.2c00212DOI Listing
August 2022

Facile fabrication of hydroxyl-functionalized hypercrosslinked polymer for sensitive determination of chlorophenols.

Food Chem 2022 Jul 14;396:133694. Epub 2022 Jul 14.

College of Science, Hebei Agricultural University, Baoding 071001, China; College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China. Electronic address:

Three hydroxyl-functionalized hypercrosslinked polymers (HCP-POL, HCP-HQ and HCP-PG) were synthesized by Friedel-Crafts reaction. The HCP-HQ displayed the largest surface area and highest adsorption capacity for chlorophenols (CPs). Thus, the HCP-HQ was further modified with magnetism to obtain M-HCP-HQ. An efficient magnetic solid-phase extraction method with M-HCP-HQ as adsorbent was developed for the first time to simultaneously extract four CPs from water and honey samples before analysis by high performance liquid chromatography-diode array detection. Under optimized conditions, the low detection limits (S/N = 3) were obtained to be 0.06-0.10 ng mL for water and 0.80-1.75 ng g for honey. The method recovery was 80.7%-119%, with relative standard deviations below 9.5%. The enrichment factors of the CPs were in the range of 57-220. The extraction mechanism could be attributed to the strong polar interaction, hydrogen bonding and π-π interactions between the M-HCP-HQ and CPs. The M-HCP-HQ based method can be served as a reliable and sensitive tool for detection CPs in water and honey samples.
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http://dx.doi.org/10.1016/j.foodchem.2022.133694DOI Listing
July 2022

Immediate Modulation of Transcutaneous Auricular Vagus Nerve Stimulation in Patients With Treatment-Resistant Depression: A Resting-State Functional Magnetic Resonance Imaging Study.

Front Psychiatry 2022 1;13:923783. Epub 2022 Jul 1.

Beijing First Hospital of Integrated Chinese and Western Medicine, Beijing, China.

Background: Previous studies found that transcutaneous auricular vagus nerve stimulation (taVNS) was clinically effective in treating a case of treatment-resistant depression (TRD). However, the brain neural mechanisms underlying the immediate effects of taVNS treatment for TRD have not been elucidated.

Materials And Methods: Differences in the amplitude of low-frequency fluctuations (ALFF) between TRD and healthy control (HC) groups were observed. The TRD group was treated with taVNS for 30 min, and changes in ALFF in the TRD group before and after immediate treatment were observed. The ALFF brain regions altered by taVNS induction were used as regions of interest to analyze whole-brain functional connectivity (FC) changes in the TRD group.

Results: A total of 44 TRD patients and 44 HCs completed the study and were included in the data analysis. Compared with the HC group, the TRD group had increased ALFF in the left orbital area of the middle frontal gyrus. After taVNS treatment, ALFF in the left orbital area of the middle frontal gyrus and right middle frontal gyrus decreased in the TRD group, while ALFF in the right orbital area of the superior frontal gyrus increased. The FC in the left orbital area of the middle frontal gyrus with left middle frontal gyrus and the right inferior occipital gyrus was significantly increased.

Conclusion: Transcutaneous auricular vagus nerve stimulation demonstrates immediate modulation of functional activity in the emotional network, cognitive control network, and visual processing cortex, and may be a potential brain imaging biomarker for the treatment of TRD.
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http://dx.doi.org/10.3389/fpsyt.2022.923783DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284008PMC
July 2022

Transcriptomic analysis reveals the key role of histone deacetylation via mediating different phytohormone signalings in fiber initiation of cotton.

Cell Biosci 2022 Jul 12;12(1):107. Epub 2022 Jul 12.

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, 450001, China.

Background: Histone deacetylation is one of the most important epigenetic modifications and plays diverse roles in plant development. However, the detailed functions and mechanisms of histone deacetylation in fiber development of cotton are still unclear. HDAC inhibitors (HDACi) have been commonly used to study the molecular mechanism underlying histone deacetylation or to facilitate disease therapy in humans through hindering the histone deacetylase catalytic activity. Trichostatin A (TSA)-the most widely used HDACi has been extensively employed to determine the role of histone deacetylation on different developmental stages of plants.

Results: Through in vitro culture of ovules, we observed that exogenous application of TSA was able to inhibit the fiber initiation development. Subsequently, we performed a transcriptomic analysis to reveal the underlying mechanisms. The data showed that TSA treatment resulted in 4209 differentially expressed genes, which were mostly enriched in plant hormone signal transduction, phenylpropanoid biosynthesis, photosynthesis, and carbon metabolism pathways. The phytohormone signal transduction pathways harbor the most differentially expressed genes. Deeper studies showed that some genes promoting auxin, Gibberellic Acid (GA) signaling were down-regulated, while some genes facilitating Abscisic Acid (ABA) and inhibiting Jasmonic Acid (JA) signaling were up-regulated after the TSA treatments. Further analysis of plant hormone contents proved that TSA significantly promoted the accumulation of ABA, JA and GA.

Conclusions: Collectively, histone deacetylation can regulate some key genes involved in different phytohormone pathways, and consequently promoting the auxin, GA, and JA signaling, whereas repressing the ABA synthesis and signaling to improve the fiber cell initiation. Moreover, the genes associated with energy metabolism, phenylpropanoid, and glutathione metabolism were also regulated by histone deacetylation. The above results provided novel clues to illuminate the underlying mechanisms of epigenetic modifications as well as related different phytohormones in fiber cell differentiation, which is also very valuable for the molecular breeding of higher quality cotton.
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http://dx.doi.org/10.1186/s13578-022-00840-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277824PMC
July 2022

Low compliance to post-screening recommendations in a family-based Helicobacter pylori screening and treatment program: A prospective cohort study.

Helicobacter 2022 Jul 11:e12912. Epub 2022 Jul 11.

Department of Gastroenterology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Background: Intra-family transmission is an important Helicobacter pylori (H. pylori) infection route. Family-based screening and treatment of H. pylori is a promising strategy. However, limited data are available on patient compliance with post-screening recommendations for such a strategy.

Materials And Methods: A prospective cohort study of families from six regions in Shandong, China, from July 2021 to February 2022 was conducted. Demographic characteristics, prior testing, and treatment for H. pylori, prior gastroscopy, symptoms, and family history were collected. Infection status of participants was determined using the C-urea breath test. Infected participants were recommended to undergo eradication treatment, confirmation testing, and gastroscopy per expert consensus. Participants were monitored for 6 months to record recommendation compliance in a real-world setting. Logistic regression models were used to analyze the factors influencing compliance with the recommendations.

Results: The study included 1173 individuals from 386 families with the overall infection rate of 36.7%. The recommendation compliance for eradication treatment, confirmation testing, and gastroscopy was 69.3% (271/391), 32.5% (88/271), and 6.1% (19/309), respectively. Factors that increased the risk of lower compliance were male sex (odds ratio [OR], 1.917, 95% confidence interval [CI], 1.233-2.981), and living in a non-urban area (OR, 1.954, 95% CI, 1.241-3.074), for treatment recommendations; having more than one infected family member (OR, 2.138, 95% CI, 1.237-3.698), and a lower family income (¥100,000-¥300,000 per year, OR, 7.247, 95% CI, 1.788-29.363; or <¥100,000 per year, OR, 7.294, 95% CI, 1.832-29.042), for confirmation testing recommendations; and being asymptomatic (OR, 3.009, 95% CI, 1.105-8.196), for gastroscopy recommendations.

Conclusions: Post-screening recommendation compliance for this family-based H. pylori screening and treatment program was unsatisfactory. Further studies focusing on pre-screening education are warranted to improve compliance.
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http://dx.doi.org/10.1111/hel.12912DOI Listing
July 2022

MiR-140 targets Wnt1 to inhibit the proliferation and enhance drug sensitivity in osteosarcoma cells.

Cell Mol Biol (Noisy-le-grand) 2022 May 22;68(1):140-146. Epub 2022 May 22.

Foot and Ankle Surgery Center, Beijing Tongren Hospital of Capital Medical University, Beijing 100005, China.

MicroRNAs (miRNAs) have been documented to function differently in numerous human cancers. Our study planned to investigate the role of microRNA-140 (miR-140) and to identify its possible target in osteosarcoma (OS) to predict their mechanism in OS. The miR-140 was down-regulated in OS, and its high expression decreased MG63 cell proliferation. At the molecular level, Wnt1 was a target of miR-140, and its expression could be suppressed by miR-140. Besides, miR-140 overexpression decreased drug resistance in OS cells treated by doxorubicin. Collectively, overexpression of miR-140 may inhibit human OS cell proliferation and may enhance drug sensitivity by direct regulation of Wnt/β-catenin signaling.
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http://dx.doi.org/10.14715/cmb/2022.68.1.18DOI Listing
May 2022

Anterior Longitudinal Ligament Flap Technique: Description of Anterior Longitudinal Ligament Opening During Anterior Lumbar Spine Surgery and Review of Vascular Complications in 189 Patients.

World Neurosurg 2022 Jul 5. Epub 2022 Jul 5.

Division of Spine Surgery, Center Hospital of the University of Nice, Alpes-Maritimes, France.

Background: One of the main concerns of anterior lumbar spine approaches are vascular complications. The aim of our study is to provide technical details about a flap technique using the anterior longitudinal ligament (ALL) when approaching the lumbar spine via an anterior corridor. This can help decrease complications by protecting the adjacent vascular structures. We also include a retrospective cohort review.

Methods: This is a retrospective bicentric study: 189 patients with a mean age of 44.2 years underwent anterior lumbar spine surgery using the ALL flap technique. Patients were diagnosed with degenerative pathologies. We treated 239 lumbar levels primarily at the L4-5 and L5-S1: 88 single-level anterior lumbar interbody fusions, 9 two-level ALIFs, 51 total disk replacements (TDR), and 41 hybrid constructs (i.e., ALIF L5S1 and TDR L4L5). Anterior approaches were performed by two senior spine surgeons. The ALL flap technique was utilized in all of these cases, by carefully dissecting the ALL, with the flap suspended using sutures. As such, this ALL flap provided a "safe corridor" to avoid any potential vascular laceration.

Results: The operative and early surgical complication rate was 3.2%. There was no arterial injury. There were only 2 minor venous lacerations (1.05%). No blood transfusion was required. Neither lacerations happened during disk space preparation.

Conclusions: Here, we provide technical details about a simple and reproducible technique using the ALL as a flap, which may help spine surgeons minimize vascular injuries during ALIF or even TDR surgeries.
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http://dx.doi.org/10.1016/j.wneu.2022.06.140DOI Listing
July 2022

Facile synthesis of magnetic hypercrosslinked polymer for the magnetic solid-phase extraction of benzoylurea insecticides from honey and apple juice samples.

Food Chem 2022 Nov 30;395:133596. Epub 2022 Jun 30.

College of Science, Hebei Agricultural University, Baoding 071001, China; College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China. Electronic address:

In this work, a novel knitting aromatic polymer (KAP) was fabricated for the first time by knitting ferrocene with 1,3,5-tris(bromomethyl)-2,4,6-trimethylbenzene via Friedel-Crafts reaction, and then the KAP was magnetically functionalized with FeO nanoparticles to produce a magnetic KAP (M-KAP). Combining M-KAP based magnetic solid-phase extraction with high performance liquid chromatography-ultraviolet detection, a simple and sensitive method was developed for simultaneous determination of six benzoylurea insecticides (BUs) in honey and apple juice samples. In the optimized conditions, a good linearity for the BUs existed in the range of 1.67-1000 ng g for honey sample and 0.500-100.0 ng mL for apple juice sample. The limits of detection were 0.500-1.50 ng g and 0.150-0.400 ng mL for honey and apple juice sample, respectively. Satisfactory recoveries were in the range of 85.5-105.5% with RSDs ≤ 8.3%. The developed method demonstrated an excellent practicability for sensitive analysis of the BUs.
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http://dx.doi.org/10.1016/j.foodchem.2022.133596DOI Listing
November 2022

Optimization of pleural multisite anesthetic technique during CT-guide microwave ablation of peripheral lung malignancy for improving treatment tolerance.

Int J Hyperthermia 2022 ;39(1):822-828

Department of Interventional Oncology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Objective: To assess the effect and safety of subpleural multisite anesthesia based on the area of thermal radiation during CT-guided lung malignancy microwave ablation (MWA) on the incidence of moderate or severe pain and the analgesic drug usage.

Materials And Methods: Consecutive patients with lung malignancies were retrospectively evaluated between January 2016 and December 2019. Patients undergoing CT-guided lung malignancy MWA were either given in the method of (a) standard subpleural puncture point anesthesia between January 2016 and June 2018 and (b) subpleural multisite anesthesia based on the area of thermal radiation between July 2018 and December 2019. The relationship between local anesthesia mode and moderate or severe pain, and pain medications usage was assessed by using multivariable logistic regression models.

Results: A total of 243 consecutive patients were included in the study. Moderate or severe pain occurred in 84 of 124 (67.7%) patients with subpleural puncture point anesthesia and in 20 of 119 (16.8%) patients with subpleural anesthesia in the area of thermal radiation (=.001). The intravenous pain medication was required in 56 of 124 (45.2%) patients with subpleural puncture point anesthesia and in 9 of 119 (7.6%) patients with subpleural multisite anesthesia based on the area of thermal radiation (=.001). Local anesthesia methods ( = 0.001), pleura-to-lesion distance (=.02) and tumor size (=.015) were independent risk factors for developing moderate or severe pain. There were no differences in adverse events and local tumor progression rate.

Conclusions: Subpleural multisite anesthesia based on the area of thermal radiation for peripheral lung malignancy MWA can result in lower intraprocedural pain compared with the subpleural puncture point anesthesia. Thus, a subpleural multisite anesthesia technique may be most helpful when performing MWA of peripheral malignancy in patients who are not sedated with general or intravenous anesthesia.
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http://dx.doi.org/10.1080/02656736.2022.2055156DOI Listing
July 2022

Clinical Efficacy of Laparoscopic Orchiopexy With the Modified Prentiss Maneuver for Non-palpable Testis Near the Internal Ring.

Front Pediatr 2022 27;10:906739. Epub 2022 May 27.

Department of Urology, Hunan Children's Hospital, Changsha, China.

Objective: To compare the clinical efficacy and safety of laparoscopic orchiopexy with the modified Prentiss maneuver (LOMPM) and laparoscopic trans-inguinal orchiopexy (LTIO) for the treatment of non-palpable testis (NPT) <1 cm from the internal ring.

Methods: Children with unilateral NPT who underwent laparoscopic orchiopexy at our center between February 2018 and January 2021 were retrospectively analyzed. According to the surgical method, they were divided into LOMPM and LTIO groups. The operation time, postoperative pain degree, postoperative complications and follow-up results were compared between the two groups.

Results: A total of 98 patients were included in this study, including 41 cases in the LOMPM group and 57 cases in the LTIO group. All patients underwent successful surgery. The LOMPM group was superior to the LTIO group in terms of postoperative testicular position (lower scrotm: 90.2 vs. 71.9%, = 0.026). There were no significant differences in operation time, postoperative pain score, and complications between the two groups. Preoperative testicular volume, postoperative testicular volume, and testicular growth rate in the LOMPM group were comparable to those in the LTIO group. There were no testicular atrophy, inguinal hernia and hydrocele in both groups after operation.

Conclusions: LOMPM was comparable in safety to LTIO, but LOMPM had a good post-operative testicular position, and was suitable for the treatment of NPT near the internal ring.
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http://dx.doi.org/10.3389/fped.2022.906739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235851PMC
May 2022

Excessive Daytime Sleepiness in Parkinson's Disease is Related to Functional Abnormalities in the Left Angular Gyrus.

Clin Neuroradiol 2022 Jun 29. Epub 2022 Jun 29.

Department of Neurology, Henan Provincial People's Hospital, 450003, Zhengzhou, Henan Province, China.

Purpose: Excessive daytime sleepiness (EDS) is a common non-motor symptom in Parkinson's disease (PD), but its neuropathology remains elusive. Our goal is to explore the potential neural substrates of EDS in a large sample of individuals with PD.

Methods: We recruited 48 PD patients with and 87 PD patients without EDS. We used resting-state functional magnetic resonance imaging to compare amplitudes of low-frequency fluctuations (ALFF) between the two groups. We also explored functional connectivity (FC) between the entire brain and regions where ALFF differed between the two groups as well as FC between selected regions of interest. Age, Part III of the Movement Disorder Society Unified Parkinson's Disease Rating Scale (MDS-UPDRS-III) score and use of dopamine receptor agonists were treated as covariates in the comparisons.

Results: EDS was associated with significantly lower ALFF in the left angular gyrus, and ALFF in this region correlated negatively with score on the Epworth Sleepiness Scale in patients with PD. EDS was also associated with significantly lower FC between the left angular gyrus and right cerebellum, based on seed-to-voxel and inter-ROI analyses.

Conclusion: Our results suggest that EDS in PD patients is associated with reduced spontaneous neural activity in the left angular gyrus and with reduced FC between the left angular gyrus and cerebellum. These findings may help understand and treat EDS in PD.
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http://dx.doi.org/10.1007/s00062-022-01190-xDOI Listing
June 2022

Ability to Inhibit H Transmission of Gemini Surfactants with Different Chain Lengths under Different Ca Circumstances.

ACS Omega 2022 Jun 7;7(24):20768-20778. Epub 2022 Jun 7.

State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation, Southwest Petroleum University, Chengdu 610500, China.

To study the ability to inhibit ion transmission of the Gemini surfactant under different Ca circumstances, three kinds of Gemini surfactants with different alkyl chain lengths are synthesized (C-4-C, = 12, 14, and 16), which are characterized using H NMR, C NMR, and Fourier transform infrared spectroscopy. To analyze the property of inhibition of the acid-rock reaction rate, surface tension and contact angle measurements and atomic force microscopy (AFM) results are obtained with different surfactants and under different Ca concentrations. Inhibition rates with different alkyl chain lengths and an acid-etched surface morphology are also studied carefully. The result shows that all cationic Gemini surfactants significantly impact the control of the reaction rate, and the reaction rate decreased remarkably by 44.4% after adding 12-4-12. The Δ and W indicate that 12-4-12 has the best adsorption ability on the rock with added Ca compared with the other two Gemini surfactants. It is revealed through the AFM that Ca can significantly change the adsorption morphology of the surfactant. The surfactant adsorption area decreased when Ca is dispersed in the solution as well. These two phenomena can lead to the reduced ability to block H of 14-4-14 and 16-4-16. However, the presence of Ca affects the adsorption area of 12-4-12 slightly. Thus, the reaction rate, including that of 12-4-12, is almost unchanged. Because 12-4-12 is adsorbed tightly on the rock surface, H can only react with the rock on the unabsorbed dot, resulting in rock surface nonuniformity after being etched, which is beneficial for maintaining the conductivity of the crack.
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http://dx.doi.org/10.1021/acsomega.2c01253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219049PMC
June 2022

Differences in Brain Activity Between Dopa-Responsive and -Unresponsive Pain in Parkinson's Disease.

Pain Ther 2022 Sep 25;11(3):959-970. Epub 2022 Jun 25.

Department of Neurology, Henan Provincial People's Hospital, Weiwu Road, Building 7, Zhengzhou, 450003, Henan Province, People's Republic of China.

Introduction: Pain in Parkinson's disease is poorly understood, and most patients with pain do not respond to dopaminergic drugs. We aimed to explore the mechanisms of dopa-responsive and -unresponsive pain by comparing such patients against patients without pain in terms of neural activity and functional connectivity in the brain.

Methods: We prospectively examined 31 Parkinson's patients with dopa-responsive pain, 51 with dopa-unresponsive pain and 93 without pain using resting-state functional magnetic resonance imaging. Neural activity was assessed in terms of the amplitude of low-frequency fluctuation, while functional connectivity was assessed based on analysis of regions of interest.

Results: Patients with dopa-unresponsive pain showed significantly higher amplitude of low-frequency fluctuation in the right parahippocampal/lingual region than patients with no pain. However, there was no amplitude difference between the dopa-responsive pain group and the no pain group. Patients with dopa-unresponsive pain also differed significantly from patients with no pain in their functional connections between the superior temporal gyrus and other areas of cerebral cortex, between amygdala and thalamus and between the amygdala and putamen. Patients with dopa-responsive pain differed significantly from patients with no pain in their functional connections between temporal fusiform cortex and cerebellum, between precentral gyrus and temporal fusiform cortex and between precentral gyrus and cerebellum.

Conclusions: Regional neural activity and functional connectivity in the brain differ substantially among Parkinson's patients with dopa-unresponsive pain, dopa-responsive pain or no pain. Our results suggest that dopa-responsive and -unresponsive pain may arise through different mechanisms, which may help guide the development of targeted therapies.
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http://dx.doi.org/10.1007/s40122-022-00404-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314530PMC
September 2022

Characteristics of statewide prescription drug monitoring programs and potentially inappropriate opioid prescribing to patients with non-cancer chronic pain: A machine learning application.

Prev Med 2022 08 21;161:107116. Epub 2022 Jun 21.

Department of Health Promotion and Behavioral Sciences, School of Public Health, University of Texas Health Science Center, Houston, TX, United States of America.

Unnecessary/unsafe opioid prescribing has become a major public health concern in the U.S. Statewide prescription drug monitoring programs (PDMPs) with varying characteristics have been implemented to improve safe prescribing practice. Yet, no studies have comprehensively evaluated the effectiveness of PDMP characteristics in reducing opioid-related potentially inappropriate prescribing (PIP) practices. The objective of the study is to apply machine learning methods to evaluate PDMP effectiveness by examining how different PDMP characteristics are associated with opioid-related PIPs for non-cancer chronic pain (NCCP) treatment. This was a retrospective observational study that included 802,926 adult patients who were diagnosed NCCP, obtained opioid prescriptions, and were continuously enrolled in plans of a major U.S. insurer for over a year. Four outcomes of opioid-related PIP practices, including dosage ≥50 MME/day and ≥90 MME/day, days supply ≥7 days, and benzodiazepine-opioid co-prescription were examined. Machine learning models were applied, including logistic regression, least absolute shrinkage and selection operation regression, classification and regression trees, random forests, and gradient boost modeling (GBM). The SHapley Additive exPlanations (SHAP) method was applied to interpret model results. The results show that among 1,886,146 NCCP opioid-related claims, 22.8% had an opioid dosage ≥50 MME/day and 8.9% ≥90 MME/day, 70.3% had days supply ≥7 days, and 10.3% were when benzodiazepine was filled ≤7 days ago. GBM had superior model performance. We identified the most salient PDMP characteristics that predict opioid-related PIPs (e.g., broader access to patient prescription history, monitoring Schedule IV controlled substances), which could be informative to the states considering the redesign of PDMPs.
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http://dx.doi.org/10.1016/j.ypmed.2022.107116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307080PMC
August 2022

Apela gene therapy alleviates pulmonary hypertension in rats.

FASEB J 2022 Jul;36(7):e22431

Department of Cardiovascular Medicine, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, China.

Pulmonary artery hypertension (PAH) is a common disease that threatens human health. At present, no treatment can cure PAH, and the prognosis is poor. Therefore, it is important to determine new targets for PAH treatment. Recently, a novel endogenous ligand Apela (ELABELA/Toddler/ELA32) of apelin peptide jejunum (APJ) receptor was identified as a possible PAH target. This study explored the potential effect of Apela gene therapy on rats with PAH. An AAV-ELA32 recombinant expression vector was constructed by molecular cloning. Purified adeno-associated virus (AAV) was injected into monocrotaline (MCT)-induced PAH rats via tail vein 1 and 2 weeks after modeling. Apela gene therapy significantly reduced the increased right ventricular systolic pressure and N-terminal pro-brain natriuretic peptide (NT-proBNP) in PAH rats. The results of histopathology and immunofluorescence showed that Apela gene therapy not only reduced the rate of pulmonary arteriole muscularization and media thickening in PAH rats but also inhibited the endothelial-to-mesenchymal transition of the pulmonary arteriole. Western blotting showed that Apela gene therapy up-regulated the expression of KLF2/eNOs and BMPRII/SMAD4 in pulmonary arterioles of PAH rats. Overall, the results show that Apela gene therapy can inhibit pulmonary arteriolar vascular remodeling and reduce pulmonary artery pressure in PAH rats. These effects may be related to KLF2/eNOs and BMPRII/SMAD4 signaling pathways. The apelinergic system may be a potential new target for the prevention and treatment of PAH.
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http://dx.doi.org/10.1096/fj.202200266RDOI Listing
July 2022

The niche-specialist and age-related oral microbial ecosystem: crosstalk with host immune cells in homeostasis.

Microb Genom 2022 06;8(6)

Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-Sen University, Guangzhou, PR China.

Although characterization of the baseline oral microbiota has been discussed, the current literature seems insufficient to draw a definitive conclusion on the interactions between the microbes themselves or with the host. This study focuses on the spatial and temporal characteristics of the oral microbial ecosystem in a mouse model and its crosstalk with host immune cells in homeostasis. The V3V4 regions of the 16S rRNA gene of 20 samples from four niches (tongue, buccal mucosa, keratinized gingiva and hard palate) and 10 samples from two life stages (adult and old) were analysed. Flow cytometry (FCM) was used to investigate the resident immune cells. The niche-specialist and age-related communities, characterized based on the microbiota structure, interspecies communications, microbial functions and interactions with immune cells, were addressed. The phylum was the major component in the oral community. The microbial community profiles at the genus level showed that the relative abundances of the genera , and were enriched in the gingiva. The abundance of the genera , and was increased in palatal samples, while the abundance of and was enriched in buccal samples. The genera , and were proportionally enriched in old samples, while and were enriched in adult samples. Network analysis showed that the genus performed as a central node in the buccal module, while in the gingiva module, the central nodes were and . FCM showed that the proportion of Th1 cells in the tongue samples (38.18 % [27.03-49.34 %]) (mean [range]) was the highest. The proportion of γδT cells in the buccal mucosa (25.82 % [22.1-29.54 %]) and gingiva (20.42 % [18.31-22.53 %]) samples was higher (<0.01) than those in the palate (14.18 % [11.69-16.67 %]) and tongue (9.38 % [5.38-13.37 %] samples. The proportion of Th2 (31.3 % [16.16-46.44 %]), Th17 (27.06 % [15.76-38.36 %]) and Treg (29.74 % [15.71-43.77 %]) cells in the old samples was higher than that in the adult samples (<0.01). Further analysis of the interplays between the microbiomes and immune cells indicated that Th1 cells in the adult group, nd Th2, Th17 and Treg cells in the old group were the main immune factors strongly associated with the oral microbiota. For example, Th2, Th17 and Treg cells showed a significantly positive correlation with age-related microorganisms such as , and , while Th1 cells showed a negative correlation. Another positive correlation occurred between Th1 cells and several commensal microbiomes such as , and . Th2, Th17 and Treg cells showed the opposite trend. Together, our findings identify the niche-specialist and age-related characteristics of the oral microbial ecosystem and the potential associations between the microbiomes and the mucosal immune cells, providing critical insights into mucosal microbiology.
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http://dx.doi.org/10.1099/mgen.0.000811DOI Listing
June 2022

Positivity-preserving high-order compact difference method for the Keller-Segel chemotaxis model.

Math Biosci Eng 2022 05;19(7):6764-6794

Institute of Applied Mathematics and Mechanics, Ningxia University, Yinchuan 750021, China.

The paper is concerned with development of an accurate and effective positivity-preserving high-order compact difference method for solving the Keller-Segel chemotaxis model, which is a kind of nonlinear parabolic-parabolic system in mathematical biology. Firstly, a stiffly-stable five-step fourth-order fully implicit compact difference scheme is proposed. The new scheme not only has fourth-order accuracy in the spatial direction, but also has fourth-order accuracy in the temporal direction, and the computational strategy for the nonlinear chemotaxis term is provided. Then, a positivity-preserving numerical algorithm is presented, which ensures the non-negativity of cell density at all time without accuracy loss. And a time advancement algorithm is established. Finally, the proposed method is applied to the numerical simulation for chemotaxis phenomena, and the accuracy, stability and positivity-preserving of the new scheme are validated with several numerical examples.
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http://dx.doi.org/10.3934/mbe.2022319DOI Listing
May 2022
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