Publications by authors named "Zhi Liu"

1,217 Publications

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Polypyrimidine tract-binding protein 1 regulates the Sertoli cell blood-testis barrier by promoting the expression of tight junction proteins.

Exp Ther Med 2021 Aug 7;22(2):847. Epub 2021 Jun 7.

Department of Histology and Embryology, School of Basic Medicine, Guizhou Medical University, Guiyang, Guizhou 550025, P.R. China.

Sertoli cells (SCs) are an important component of spermatogenic tubules. The blood-testis barrier (BTB) is composed of SCs and is necessary for the development and maturity of spermatogenic cells. When the tight connection between SCs is destroyed, the BTB loses its integrity, leading to impaired spermatogenesis. Polypyrimidine tract-binding protein 1 (PTBP1) is a key protein involved in precursor mRNA splicing and selective splicing events, which directly affects tumor cell proliferation and influences the formation of the blood-tumor barrier by regulating the expression levels of tight junction-associated proteins. The present study revealed that the expression of PTBP1 was downregulated following a decrease in spermatogenic activity at the phase of senescence. TM4 cells were transfected with lentivirus-short hairpinRNA-PTBP1 to evaluate the effect of silencing PTBP1 on the expression levels of tight junction proteins and the integrity of tight junctions between adjacent SCs. Western blot analysis indicated that the expression levels of Zonula occludens 1, occludin and claudin-5 decreased significantly due to silencing of PTBP1 in SCs. Through detecting trans-epithelial electrical resistance, it was revealed that silencing of PTBP1 broke the integrity of tight junctions between adjacent SCs. The results suggested that PTBP1 maintained the integrity of the BTB by promoting the expression levels of tight junction-associated proteins and revealed the possible mechanism of PTBP1 in regulating spermatogenesis.
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http://dx.doi.org/10.3892/etm.2021.10279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210256PMC
August 2021

Discovery of Orally Bioavailable Ligand Efficient Quinazolindiones as Potent and Selective Tankyrases Inhibitors.

ACS Med Chem Lett 2021 Jun 13;12(6):1005-1010. Epub 2021 May 13.

Virtual PoC DPU, Alternative Discovery and Development, IVIVT, Platform Technologies and Sciences, GlaxoSmithKline, 709 Swedeland Road, King of Prussia, Pennsylvania 19406, United States.

We report herein the discovery of quinazolindiones as potent and selective tankyrase inhibitors. Elucidation of the structure-activity relationship of the lead compound led to truncated analogues that have good potency in cells, pharmacokinetic (PK) properties, and excellent selectivity. Compound exhibited excellent potencies in cells and proliferation studies, good selectivity, activities, and an excellent PK profile. Compound also inhibited H292 xenograft tumor growth in nude mice. The synthesis, biological, pharmacokinetic, efficacy studies, and safety profiles of compounds are presented.
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http://dx.doi.org/10.1021/acsmedchemlett.1c00160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201761PMC
June 2021

Biphasic anaphylaxis manifested as type I Kounis syndrome induced by ingestion of raw fish gallbladder: A case report.

World J Emerg Med 2021 ;12(3):238-240

Department of Emergency Medicine, the First Affiliated Hospital, China Medical University, Shenyang 110001, China.

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http://dx.doi.org/10.5847/wjem.j.1920-8642.2021.03.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188290PMC
January 2021

The Efficacy and Safety of Esmolol for Septic Shock: A Systematic Review and Meta-analysis of Randomized Controlled Trials.

Front Pharmacol 2021 1;12:682232. Epub 2021 Jun 1.

Beijing Dongfang Hospital, Beijing University of Traditional Chinese Medicine, Beijing, China.

The meta-analysis aims to identify whether septic shock patients can benefit from esmolol. The relevant studies from MEDLINE, Cochrane Library, Embase were searched by two independent investigators using a variety of keywords. Stata software (version 12.0, Stata Corp LP, College Station, TX, United States)was used for statistical analysis. A total of 14 studies were identified and incorporated into the meta-analysis. For overall analysis, the treatment of esmolol was associated with decreased 28-day mortality (RR = 0.66, 95% CI = 0.56-0.77, < 0.001). Meanwhile, our analysis found that, esmolol could decrease HR (SMD: -1.70; 95% CI: [-2.24-(-1.17)], cTnI (SMD: -1.61; 95% CI: [-2.06-(-1.16)] compared with standard treatment. No significant differences between the two groups were found in MAP, Lac, CI, and SVI. The findings of this meta-analysis intend to demonstrate that septic shock patients with high heart beats rate might be benefit from esmolol treatment despite enough fluid resuscitation. While, dependent on the study published, with the further development of septic shock, the positive impact of esmolol varies. The appropriate heart rate change interval cannot be confirmed, further high-quality and large-scale RCTs should be performed to verify it and screening more suitable heart rate levels. CRD42021239513.
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http://dx.doi.org/10.3389/fphar.2021.682232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204042PMC
June 2021

Exosomes derived from LPS-induced MHs cells prompted an inflammatory response in sepsis-induced acute lung injury.

Respir Physiol Neurobiol 2021 Jun 6;292:103711. Epub 2021 Jun 6.

Emergency Department, First Hospital of China Medical University, Shenyang City, 110001, Liaoning Province, China. Electronic address:

Exosome is a novel tool with an essential role in cell communication. However, its role in the pathogenesis of sepsis-induced acute lung injury is currently unknown. Here, we first found that lipopolysaccharide (LPS) could up-regulate the expression of pro-inflammatory cytokines and promote exosomes release in the murine alveolar macrophage cell line (MHs cells). Moreover, we found MHs cells derived exosomes also maintain the pro-inflammatory effect after LPS stimulation. Treating with hydrochloride hydrate (GW4869) could dose-dependently downregulated the release of exosomes and inhibited the upregulation of inflammatory cytokines in MHs cells with LPS treatment. Also, we further identified GW4869 administration induced the remission of histopathologic changes, the reduction of pro-inflammatory cytokines in lung tissue, and inhibit serum exosomes release. These results indicate that the downregulation of exosome release by GW4869 might protect lung tissue from LPS induced injury through the suppression of excessive inflammatory responses, suggesting its potential therapeutic effects on sepsis-induced acute lung injury.
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http://dx.doi.org/10.1016/j.resp.2021.103711DOI Listing
June 2021

Ginsenoside Rh1 Improves Type 2 Diabetic Nephropathy through AMPK/PI3K/Akt-Mediated Inflammation and Apoptosis Signaling Pathway.

Am J Chin Med 2021 May 27:1-19. Epub 2021 May 27.

College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, P. R. China.

Although ginseng ( C.A. Meyer) has received extensive attention in the treatment and prevention of type 2 diabetes mellitus (T2DM) in the past few decades, there are few studies on the complications of T2DM. At present, obesity-linked diabetic nephropathy (DN) has become the most prevailing element of the end-stage renal failure in the world. The aim of this work is to evaluate the ameliorative effects of ginsenoside Rh1 (G-Rh1) on DN induced by high fat diet plus streptozotocin (HFD/STZ) through some potential and combined mechanisms of action. The results showed that G-Rh1 treatment at 5 and 10 mg/kg for 8 weeks exerted excellent effects in controlling fasting blood glucose (FBG), improving glucose tolerance, and increasing insulin level. In addition, G-Rh1 effectively prevents the excessive production of advanced glycation end products (AGEs), a diabetic nephropathy marker, in HFD/STZ induced DN mice. Meanwhile, oxidation indicators including SOD, GSH, and MDA were improved by G-Rh1 treatment to varying degrees. It is worth noting that G-Rh1 not only inhibits the secretion of Nox1 and Nox4 in kidney tissues, but also has an inhibitory effect on inflammatory factors and NF-[Formula: see text]B signaling pathway. Importantly, further in-depth research on molecular mechanisms provides vital evidence that the ameliorative effect of G-Rh1 on DN is related to the inhibition of apoptosis and the AMPK/PI3K/Akt signaling pathway. In summary, G-Rh1 may be of great value in improving the treatment of DN although more experimental data is needed.
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http://dx.doi.org/10.1142/S0192415X21500580DOI Listing
May 2021

Cardiovascular safety of long-term anti-obesity drugs in subjects with overweight or obesity: a systematic review and meta-analysis.

Eur J Clin Pharmacol 2021 May 27. Epub 2021 May 27.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.

Purpose: Anti-obesity therapy can reduce body weight; however, it is not clear whether it can reduce major adverse cardiovascular events (MACEs). We conducted a systematic review and meta-analysis to assess the effect of long-term anti-obesity drugs on MACEs in individuals with overweight or obesity.

Methods: The MEDLINE, Embase, and Cochrane Library databases and clinical trial registries ( https://clinicaltrials.gov ) were searched up to 3 May 2021 for randomized controlled trials (RCT) that compared anti-obesity drugs with controls and reported cardiovascular events in subjects with overweight or obesity. Heterogeneity was described by the I value. The Mantel-Haenszel randomized effects model was adopted to calculate risk ratios (RR) and weighted mean differences (WMD). Sensitivity analysis was used to assess the stability of the effects. Publication bias was assessed by Begg's funnel plot and Egger's test. The Cochrane Collaboration risk-of-bias tool was used to evaluate the bias of each included RCT.

Results: Twelve articles were included; 21,391 and 17,618 subjects were in the anti-obesity drug and placebo groups, respectively. There was no difference in MACEs between the anti-obesity drug and placebo groups (RR 0.99; 95% CI: 0.88-1.12). Compared with placebo, anti-obesity interventions reduced body weight (WMD: - 3.96 kg; 95% CI: - 4.89, - 3.03) and improved lipid and blood glucose profiles. The intervention also did not increase the incidence of depression or anxiety or the risk of suicidal ideation.

Conclusion: Long-term anti-obesity drugs did not show a benefit in lowering MACEs in overweight or obese subjects, although the drugs resulted in a decrease in body weight and improved cardiometabolic parameters.
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http://dx.doi.org/10.1007/s00228-021-03160-7DOI Listing
May 2021

A nomogram for predicting metabolic steatohepatitis: The combination of NAMPT, RALGDS, GADD45B, FOSL2, RTP3, and RASD1.

Open Med (Wars) 2021 17;16(1):773-785. Epub 2021 May 17.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.

Objective: To identify differentially expressed and clinically significant mRNAs and construct a potential prediction model for metabolic steatohepatitis (MASH).

Method: We downloaded four microarray datasets, GSE89632, GSE24807, GSE63067, and GSE48452, from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) analysis and weighted gene co-expression network analysis were performed to screen significant genes. Finally, we constructed a nomogram of six hub genes in predicting MASH and assessed it through receiver operating characteristic (ROC) curve, calibration plot, and decision curve analysis (DCA). In addition, qRT-PCR was used for relative quantitative detection of RNA in QSG-7011 cells to further verify the expression of the selected mRNA in fatty liver cells.

Results: Based on common DEGs and brown and yellow modules, seven hub genes were identified, which were NAMPT, PHLDA1, RALGDS, GADD45B, FOSL2, RTP3, and RASD1. After logistic regression analysis, six hub genes were used to establish the nomogram, which were NAMPT, RALGDS, GADD45B, FOSL2, RTP3, and RASD1. The area under the ROC of the nomogram was 0.897. The DCA showed that when the threshold probability of MASH was 0-0.8, the prediction model was valuable to GSE48452. In QSG-7011 fatty liver model cells, the relative expression levels of NAMPT, GADD45B, FOSL2, RTP3, RASD1 and RALGDS were lower than the control group.

Conclusion: We identified seven hub genes NAMPT, PHLDA1, RALGDS, GADD45B, FOSL2, RTP3, and RASD1. The nomogram showed good performance in the prediction of MASH and it had clinical utility in distinguishing MASH from simple steatosis.
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http://dx.doi.org/10.1515/med-2021-0286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130015PMC
May 2021

[Performance Verification of Plasma Renin and Aldosterone Examination with Chemiluminescence Immunoassay and Its Screening Efficacy for Primary Aldosteronism].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 May;52(3):472-476

Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, China.

Objective: To evaluate the performance of chemiluminescence immunoassay (CLIA) in examining renin and aldosterone and to determine its value for screening for primary aldosteronism (PA).

Methods: According to the relevant documents of Clinical and Laboratory Standards Institute (CLSI), we verified the precision, linear range and carryover rate of examining renin and aldosterone with CLIA. The study included 91 suspected PA patients, using two methods, CLIA and radioimmunoassay (RIA), to examine renin and aldosterone levels in order to compare the correlation between the two methods and their value for PA screening.

Results: The precision, linear range and carryover rate of examining renin and aldosterone with CLIA met the requirements. In patients with suspected PA, the correlation coefficients of renin, aldosterone and aldosterone-to-renin ratio (ARR) assessed by CLIA and RIA were 0.901, 0.861 and 0.847 respectively (all <0.001). When the patients were in the upright position and the ARR was 5.636 (ng/dL)/(ng/L), the CLIA method had 79.1% sensitivity and 93.7% specificity for PA screening; when ARR was 14.084 (ng·dL )/(ng·[mL·h] ), the RIA method had 93.0% sensitivity and 83.3% specificity for PA screening. When the patients were in the supine position, and the ARR was 5.640 (ng/dL)/(ng/L), the CLIA method had 97.7% sensitivity and 81.2% specificity for PA screening; when ARR was 33.494 (ng·dL )/(ng·[mL·h] ), RIA had 95.3% sensitivity and 70.8% specificity for PA screening .

Conclusion: The performance of the CLIA kit in assessing the concentration of renin and aldosterone meets the clinical requirements. Regarding preliminary PA screening, upright-position ARR had higher specificity, but lower sensitivity compared with supine-position ARR.
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http://dx.doi.org/10.12182/20210560505DOI Listing
May 2021

Functional and structural characterization of a two-MAb cocktail for delayed treatment of enterovirus D68 infections.

Nat Commun 2021 05 18;12(1):2904. Epub 2021 May 18.

CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

Enterovirus D68 (EV-D68) is an emerging pathogen associated with respiratory diseases and/or acute flaccid myelitis. Here, two MAbs, 2H12 and 8F12, raised against EV-D68 virus-like particle (VLP), show distinct preference in binding VLP and virion and in neutralizing different EV-D68 strains. A combination of 2H12 and 8F12 exhibits balanced and potent neutralization effects and confers broader protection in mice than single MAbs when given at onset of symptoms. Cryo-EM structures of EV-D68 virion complexed with 2H12 or 8F12 show that both antibodies bind to the canyon region of the virion, creating steric hindrance for sialic acid receptor binding. Additionally, 2H12 binding can impair virion integrity and trigger premature viral uncoating. We also capture an uncoating intermediate induced by 2H12 binding, not previously described for picornaviruses. Our study elucidates the structural basis and neutralizing mechanisms of the 2H12 and 8F12 MAbs and supports further development of the 2H12/8F12 cocktail as a broad-spectrum therapeutic agent against EV-D68 infections in humans.
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http://dx.doi.org/10.1038/s41467-021-23199-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131599PMC
May 2021

miRNAs and Leukotrienes in Respiratory Syncytial Virus Infection.

Front Pediatr 2021 29;9:602195. Epub 2021 Apr 29.

Department of Pediatrics, Children's Digital Health and Data Center, Zhongnan Hospital of Wuhan University, Wuhan, China.

MicroRNAs (miRNAs) are small, non-coding RNAs that regulate posttranscription by binding to 3'-untranslated regions of target mRNAs. Recent functional studies have elucidated mechanisms that miRNAs regulate leukotriene synthesis by perturbing arachidonic acid metabolism. Both microarrays and high-throughput sequencing revealed distinct differential expression of miRNAs in children with respiratory syncytial virus (RSV) infection compared with healthy controls. Abnormal miRNA expression may contribute to higher leukotriene levels, which is associated with airway hyperreactivity. Targeting miRNAs may benefit to restore the homeostasis of inflammatory reaction and provide new strategies to alleviate airway hyperreactivity induced by RSV. In this article, we provide an overview of the current knowledge about miRNAs modulating leukotrienes through regulation of arachidonic acid metabolism with a special focus on miRNAs aberrantly expressed in children with RSV infection.
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http://dx.doi.org/10.3389/fped.2021.602195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116547PMC
April 2021

MDMPR: Mouse Developmental and Metabolic Phenotype Repository.

Yi Chuan 2021 Apr;43(4):375-384

Cambridge-Suda Genomic Resource Center, Soochow University, Suzhou 215123, China.

Mouse Developmental and Metabolic Phenotype Repository (MDMPR) is an open access, real-time database which dedicates to share mouse resources and phenotype data. MDMPR is supported by the National Key Research and Development Project "Establishment of Mouse Developmental and Metabolic Phenotype Repository" within the Key Project of "Developmental Programming and Its Metabolic Regulation" from the Ministry of Science and Technology of the People's Republic of China's program. In the next 5 years, MDMPR will create 500 mutant mouse models related to development and metabolism, perform standard phenotyping analysis, and establish a phenotype database. MDMPR is a combination of resources and data repository, has several sub-systems, including the ES cell database, the project management system, the breeding management system, the sperm bank management system and the phenotyping database. These systems digitalize all data and ensure their authenticity in real-time. Besides the gradual increase of data during the project, MDMPR will also integrate other resources, such as human KO ES cell database, STRING database, database of Core Transcriptional Regulatory Circuitries and Enhancer-Indel database. MDMPR is anticipated to contribute to various areas of developmental and metabolic research to investigators through more convenient accesses to the resources and data in one-stop manner, thereby accelerating the research processes and ultimately serving the medical causes of human health.
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http://dx.doi.org/10.16288/j.yczz.21-005DOI Listing
April 2021

Neural Adaptive Self-Triggered Control for Uncertain Nonlinear Systems With Input Hysteresis.

IEEE Trans Neural Netw Learn Syst 2021 May 10;PP. Epub 2021 May 10.

The issue of neural adaptive self-triggered tracking control for uncertain nonlinear systems with input hysteresis is considered. Combining radial basis function neural networks (RBFNNs) and adaptive backstepping technique, an adaptive self-triggered tracking control approach is developed, where the next trigger instant is determined by the current information. Compared with the event-triggered control mechanism, its biggest advantage is that it does not need to continuously monitor the trigger condition of the system, which is convenient for physical realization. By the proposed controller, the hysteresis's effect can be compensated effectively and the tracking error can be bounded by an explicit function of design parameters. Simultaneously, all other signals in the closed-loop system can be remaining bounded. Finally, two examples are presented to verify the effectiveness of the proposed method.
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http://dx.doi.org/10.1109/TNNLS.2021.3072784DOI Listing
May 2021

Altering Hydrogenation Pathways in Photocatalytic Nitrogen Fixation by Tuning Local Electronic Structure of Oxygen Vacancy with Dopant.

Angew Chem Int Ed Engl 2021 May 7. Epub 2021 May 7.

Hefei National Laboratory for Physical Sciences at the Microscale, Collaborative Innovation Center of Chemistry for Energy Materials (iChEM), School of Chemistry and Materials Science, National Synchrotron Radiation Laboratory, and, CAS Center for Excellence in Nanoscience Institution, University of Science and Technology of China, Hefei, Anhui, 230026, China.

To avoid the energy-consuming step of direct N≡N bond cleavage, photocatalytic N fixation undergoing the associative pathways has been developed for mild-condition operation. However, it is a fundamental yet challenging task to gain comprehensive understanding on how the associative pathways (i.e., alternating vs. distal) are influenced and altered by the fine structure of catalysts, which eventually holds the key to significantly promote the practical implementation. Herein, we introduce Fe dopants into TiO nanofibers to stabilize oxygen vacancies and simultaneously tune their local electronic structure. The combination of in situ characterizations with first-principles simulations reveals that the modulation of local electronic structure by Fe dopants turns the hydrogenation of N from associative alternating pathway to associative distal pathway. This work provides fresh hints for rationally controlling the reaction pathways toward efficient photocatalytic nitrogen fixation.
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http://dx.doi.org/10.1002/anie.202104001DOI Listing
May 2021

SARS-CoV-2 encoded microRNAs are involved in the process of virus infection and host immune response.

J Biomed Res 2021 Jan;35(3):216-227

State Key Laboratory of Reproductive Medicine, Center of Global Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China.

The outbreak of COVID-19 caused by SARS-CoV-2 is spreading worldwide, with the pathogenesis mostly unclear. Both virus and host-derived microRNA (miRNA) play essential roles in the pathology of virus infection. This study aims to uncover the mechanism for SARS-CoV-2 pathogenicity from the perspective of miRNA. We scanned the SARS-CoV-2 genome for putative miRNA genes and miRNA targets and conducted experiments to validate the virus-encoded miRNAs and their regulatory role on the putative targets. One of such virus-encoded miRNAs, MR147-3p, was overexpressed that resulted in significantly decreased transcript levels of all of the predicted targets in human, , , , and in the virus-infected cells. The analysis showed that the immune response and cytoskeleton organization are two of the most notable biological processes regulated by the infection-modulated miRNAs. Additionally, the genomic mutation of SARS-CoV-2 contributed to the changed miRNA repository and targets, suggesting a possible role of miRNAs in the attenuated phenotype of SARS-CoV-2 during its evolution. This study provided a comprehensive view of the miRNA-involved regulatory system during SARS-CoV-2 infection, indicating possible antiviral therapeutics against SARS-CoV-2 through intervening miRNA regulation.
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http://dx.doi.org/10.7555/JBR.35.20200154DOI Listing
January 2021

AutoImplant 2020 -First MICCAI Challenge on Automatic Cranial Implant Design.

IEEE Trans Med Imaging 2021 May 3;PP. Epub 2021 May 3.

The aim of this paper is to provide a comprehensive overview of the MICCAI 2020 AutoImplant Challenge1. The approaches and publications submitted and accepted within the challenge will be summarized and reported, highlighting common algorithmic trends and algorithmic diversity. Furthermore, the evaluation results will be presented, compared and discussed in regard to the challenge aim: seeking for low cost, fast and fully automated solutions for cranial implant design. Based on feedback from collaborating neurosurgeons, this paper concludes by stating open issues and post-challenge requirements for intra-operative use. The codes can be found at https://github.com/Jianningli/tmi.
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http://dx.doi.org/10.1109/TMI.2021.3077047DOI Listing
May 2021

The role of recombinant human brain natriuretic peptide on the cardiac output of patients with acute decompensated heart failure using Guyton venous return curve: A STROBE-compliant retrospective study.

Medicine (Baltimore) 2021 Apr;100(17):e25492

Department of Intensive Care Unit, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Abstract: rbBNP has positive cardiac effects in patients with acute decompensated heart failure, but its effects on the systemic venous circulation are not known.A single-center retrospective, self-controlled study was conducted on 14 patients undergone recombinant human brain natriuretic peptide (rhBNP) treatment between January 1, 2015 to December 31, 2018.The cardiac output (CO) significantly increased from 3.75 ± 1.14 L min-1 to 4.24 ± 0.97 L min-1 30 minutes after rbBNP infusion, and to 4.20 ± 1.19 L min-1 3 hours later. The systemic vascular resistance significantly decreased from 18.85 ± 7.66 mm Hg min L-1 to 14.62 ± 6.13 mm Hg min L-1 30 minutes. The resistance to venous return (VR) significantly decreased from 5.93 ± 4.97 mm Hg min L-1 to 4.46 ± 1.53 mmHg min L-1 3 hours later. The mean systemic filling pressure significantly decreased from 32.71 ± 20.00 mm Hg to 28.254 ± 6.09 mm Hg 3 hours later.The role of rhBNP on CO was to reduce the peripheral circulation resistance at 30 minutes after rhBNP infusion and to reduce the resistance to VR at 3 hours later.This trial is registered at ChiCTR: ID ChiCTR1900024562.
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http://dx.doi.org/10.1097/MD.0000000000025492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084054PMC
April 2021

[Prediction of epilepsy based on common spatial model algorithm and support vector machine double classification].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2021 Feb;38(1):39-46

School of Information Science and Engineering, Shandong University, Qingdao, Shandong 266237, P.R.China.

At present the prediction method of epilepsy patients is very time-consuming and vulnerable to subjective factors, so this paper presented an automatic recognition method of epilepsy electroencephalogram (EEG) based on common spatial model (CSP) and support vector machine (SVM). In this method, the CSP algorithm for extracting spatial characteristics was applied to the detection of epileptic EEG signals. However, the algorithm did not consider the nonlinear dynamic characteristics of the signals and ignored the time-frequency information, so the complementary characteristics of standard deviation, entropy and wavelet packet energy were selected for the combination in the feature extraction stage. The classification process adopted a new double classification model based on SVM. First, the normal, interictal and ictal periods were divided into normal and paroxysmal periods (including interictal and ictal periods), and then the samples belonging to the paroxysmal periods were classified into interictal and ictal periods. Finally, three categories of recognition were realized. The experimental data came from the epilepsy study at the University of Bonn in Germany. The average recognition rate was 98.73% in the first category and 99.90% in the second category. The experimental results show that the introduction of spatial characteristics and double classification model can effectively solve the problem of low recognition rate between interictal and ictal periods in many literatures, and improve the identification efficiency of each period, so it provides an effective detecting means for the prediction of epilepsy.
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http://dx.doi.org/10.7507/1001-5515.201911042DOI Listing
February 2021

Deafness enhances perceptual span size in Chinese reading: Evidence from a gaze-contingent moving-window paradigm.

Psych J 2021 Apr 25. Epub 2021 Apr 25.

Department of Psychology, Beijing Normal University, Beijing, China.

Using a gaze-contingent moving-window paradigm, we investigated whether/how deafness affects perceptual processing in Chinese reading. Besides the manipulation of window size, word length of sentences used in the experiment was also manipulated to check whether deafness enhanced the word length effect on perceptual span. Significant interactions of window constraints and deafness and a three-way interaction were observed on reading rate. Smaller effects of window constraints for deaf Chinese readers and nonreliable three-way interactions were observed on forward saccade length. This suggests that deaf Chinese readers exhibit a larger perceptual span, and word length affected the span from which information was acquired for comprehension whereas both deafness and word length might have little impact on the span from which information is acquired for oculomotor targeting during natural reading of Chinese.
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http://dx.doi.org/10.1002/pchj.442DOI Listing
April 2021

Exosomal lncRNA-p21 derived from mesenchymal stem cells protects epithelial cells during LPS-induced acute lung injury by sponging miR-181.

Acta Biochim Biophys Sin (Shanghai) 2021 May;53(6):748-757

Emergency Department, The First Hospital of China Medical University, Shenyang 110001, China.

Long noncoding RNAs (lncRNAs) act as essential regulators of various diseases. However, the functions of lncRNAs in sepsis-induced acute lung injury (SALI) remain unclear. Here, we found that lipopolysaccharide could upregulate lncRNA-p21 expression in mesenchymal stem cells (MSCs) in a time- and dose-dependent manner and that lncRNA-p21 was packaged into exosomes. Furthermore, we demonstrated that treatment with exosomal lncRNA-p21 could increase the expression of sirtuin 1 (SIRT1) to protect MLE-12 cells from apoptosis during sepsis. Moreover, we identified SIRT1 as a direct target of miR-181 and found that the level of SIRT1 was negatively correlated with the level of miR-181. The luciferase reporter assay also confirmed the negative correlation between the levels of miR-181 and lncRNA-p21. Our results showed that the lncRNA-p21-induced downregulation of miR-181 might suppress epithelial cell apoptosis and alleviate lung tissue injury by upregulating SIRT1 expression, suggesting the potential therapeutic effects of lncRNA-p21 on SALI. In conclusion, we found that the novel lncRNA-p21/miR-181/SIRT1 pathway may play an important role in the progression of SALI, and MSC-derived exosomes may be a new therapeutic strategy for this disease.
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http://dx.doi.org/10.1093/abbs/gmab043DOI Listing
May 2021

Pallidal versus subthalamic deep-brain stimulation for meige syndrome: a retrospective study.

Sci Rep 2021 Apr 22;11(1):8742. Epub 2021 Apr 22.

Department of Neurosurgery, Peking University People's Hospital, 11th Xizhimen South St., Beijing, 100044, China.

Deep-brain stimulation (DBS) is an effective treatment for patients with Meige syndrome. The globus pallidus interna (GPi) and the subthalamic nucleus (STN) are accepted targets for this treatment. We compared 12-month outcomes for patients who had undergone bilateral stimulation of the GPi or STN. Forty-two Asian patients with primary Meige syndrome who underwent GPi or STN neurostimulation were recruited between September 2017 and September 2019 at the Department of Neurosurgery, Peking University People's Hospital. The primary outcome was the change in motor function, including the Burke-Fahn-Marsden Dystonia Rating Scale movement (BFMDRS-M) and disability subscale (BFMDRS-D) at 3 days before DBS (baseline) surgery and 1, 3, 6, and 12 months after surgery. Secondary outcomes included health-related quality of life, sleep quality status, depression severity, and anxiety severity at 3 days before and 12 months after DBS surgery. Adverse events during the 12 months were also recorded. Changes in BFMDRS-M and BFMDRS-D scores at 1, 3, 6, and 12 months with DBS and without medication did not significantly differ based on the stimulation target. There were also no significant differences in the changes in health-related quality of life (36-Item Short-Form General Health Survey) and sleep quality status (Pittsburgh Sleep Quality Index) at 12 months. However, there were larger improvements in the STN than the GPi group in mean score changes on the 17-item Hamilton depression rating scale (- 3.38 vs. - 0.33 points; P = 0.014) and 14-item Hamilton anxiety rating scale (- 3.43 vs. - 0.19 points; P < 0.001). There were no significant between-group differences in the frequency or type of serious adverse events. Patients with Meige syndrome had similar improvements in motor function, quality of life and sleep after either pallidal or subthalamic stimulation. Depression and anxiety factors may reasonably be included during the selection of DBS targets for Meige syndrome.
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http://dx.doi.org/10.1038/s41598-021-88384-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062505PMC
April 2021

TriBAFF-CAR-T cells eliminate B-cell malignancies with BAFFR-expression and CD19 antigen loss.

Cancer Cell Int 2021 Apr 17;21(1):223. Epub 2021 Apr 17.

Department of Hematology, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong Province, 510317, China.

Background: To investigate the effect of TriBAFF-CAR-T cells on hematological tumor cells.

Methods: TriBAFF-CAR-T and CD19-CAR-T cells were co-cultured with BAFFR-bearing B-cell malignancies at different effector/target ratios to evaluate the anti-tumor effects. In vivo, TriBAFF-CAR-T and CD19-CAR-T cells were intravenously injected into Raji-luciferase xenograft mice. CD19 antigens losing lymphoblasts was simulated by Raji knocking out CD19 (CD19) to investigate the effect of TriBAFF-CAR-T cells on CD19 Raji.

Results: Both TriBAFF-CAR-T and CD19-CAR-T cells significantly induced the lysis of Raji, BALL-1, and Jeko-1. Moreover, when CD19-CAR-T cells specifically caused the lysis of K562 with overexpressed CD19, the lethal effect of TriBAFF-CAR-T cells was also specific for BAFFR-bearing K562 with increasing levels of interleukin-2 and INF-γ. The TriBAFF-CAR-T have the same effect with CD19-CAR-T cells in treating Raji xenofraft mice. TriBAFF-CAR-T cells also have great effect in CD19 Raji cells.

Conclusions: In this study, we successfully constructed novel TriBAFF-CAR-T cells to eliminate BAFFR-bearing and CD19 antigen loss in hematological tumor cells.
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http://dx.doi.org/10.1186/s12935-021-01923-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052726PMC
April 2021

Reduced inspiratory muscle strength increases pneumonia in patients with acute myocardial infarction.

Ann Phys Rehabil Med 2021 Apr 12:101511. Epub 2021 Apr 12.

Department of Cardiology, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of Coronary Heart Disease Prevention, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510100, China; The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, China. Electronic address:

Background: Inspiratory muscle strength is associated with pneumonia in patients after surgery or those with subacute stroke. However, inspiratory muscle strength in patients with acute myocardial infarction (AMI) has not been studied.

Objective: To evaluate the predictive value of inspiratory muscle strength for pneumonia in patients with AMI.

Methods: Patients with AMI were consecutively enrolled from March 2019 to September 2019. Measurements of maximal inspiratory pressure (MIP) were used to estimate inspiratory muscle strength and mostly were taken within 24 hr after culprit-vessel revascularization. Patients were divided into 3 groups by MIP tertile (T1: < 56.1 cm H2O, n = 88; T2: 56.1-84.9 cm H2O, n = 88; T3: > 84.9 cm H2O, n=89). The primary endpoint was in-hospital pneumonia.

Results: Among 265 enrolled patients, pneumonia developed in 26 (10%). The rates of pneumonia were decreased from MIP T1 to T3 (T1: 17%, T2: 10%, T3: 2%, P = 0.004). In-hospital all-cause mortality and major adverse cardiovascular events (MACEs) did not differ between groups. Multivariate logistic regression confirmed increased MIP associated with reduced risk of pneumonia (odds ratio 0.78, 95% confidence interval 0.65-0.94, P = 0.008). Receiver operating characteristic curve analysis indicated that MIP had good performance for predicting in-hospital pneumonia, with an area under the curve of 0.72 (95% confidence interval 0.64-0.81, P < 0.001).

Conclusions: The risk of pneumonia but not in-hospital mortality and MACEs was increased in AMI patients with inspiratory muscle weakness. Future study focused on training inspiratory muscle may be helpful.
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http://dx.doi.org/10.1016/j.rehab.2021.101511DOI Listing
April 2021

Coordinated ramp signal optimization framework based on time series flux-correlation analysis.

PeerJ Comput Sci 2021 25;7:e446. Epub 2021 Mar 25.

College of Computer Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang, China.

Urban expressways provide an effective solution to traffic congestion, and ramp signal optimization can ensure the efficiency of expressway traffic. The existing methods are mainly based on the static spatial distance between mainline and ramp to achieve multi-ramp coordinated signal optimization, which lacks the consideration of the dynamic traffic flow and lead to the long time-lag, thus affecting the efficiency. This article develops a coordinated ramp signal optimization framework based on mainline traffic states. The main contribution was traffic flow-series flux-correlation analysis based on cross-correlation, and development of a novel multifactorial matric that combines flow-correlation to assign the excess demand for mainline traffic. Besides, we used the GRU neural network for traffic flow prediction to ensure real-time optimization. To obtain a more accurate correlation between ramps and congested sections, we used gray correlation analysis to determine the percentage of each factor. We used the Simulation of Urban Mobility simulation platform to evaluate the performance of the proposed method under different traffic demand conditions, and the experimental results show that the proposed method can reduce the density of mainline bottlenecks and improve the efficiency of mainline traffic.
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http://dx.doi.org/10.7717/peerj-cs.446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022601PMC
March 2021

The study on the clinical effectiveness and safety of traditional Chinese medicine acupoint catgut embedding guided by musculoskeletal ultrasound in the treatment of nerve root sciatica: A protocol for systematic review and meta-analysis of randomized controlled trials.

Medicine (Baltimore) 2021 Apr;100(13):e25387

Department of Ultrasound.

Background: Nerve root sciatica (NRS) is a common orthopedic disease, which usually occurs between 20 and 40 years of age, and the incidence rate is increasing year by year and is being younger. The disease has no special effect of treatment, clinically generally taking the symptomatic treatment, such as taking short-term glucocorticoids, sedatives, analgesics, and so on. Long-term use of drugs will adversely affect the patient's gastrointestinal tract, liver, and kidney function. The surgical treatment has a high risk of surgery, high cost, side effects, and other problems, so the choice of treatment method has always been a difficult problem in clinical and scientific research. The study shows that 90% of patients with sciatica can be cured by non-surgical treatment, so conservative therapy is often used in the treatment of sciatica, traditional Chinese medicine treatment methods in the treatment of NRS has been widely used, which has achieved good results, but there is no evidence of evidence-based medicine. Therefore, this study uses systematic evaluation to conduct the scientific evaluation of the clinical effectiveness and safety of traditional Chinese medicine acupoint catgut embedding guided by musculoskeletal ultrasound in the treatment of NRS, and provide evidence-based medical evidence support for the treatment of NRS.

Methods: Using the computer to retrieve the PubMed, ScienceDirect, Web of Science, Embase, Cochrane Library, CNKI, VIP, WANFANG Database, and CBM. Using the subject words and terminology words to retrieve the Chinese-English database and retrieve a randomized controlled study on the clinical effectiveness and safety of traditional Chinese medicine acupoint catgut embedding guided by musculoskeletal ultrasound in the treatment of NRS, and the range of search time is January 1990 to January 2021. The searched literature is screened and evaluated by two researchers respectively according to the inclusion and exclusion criteria. If there is disagreement, discussing it with the third researcher to determine the final inclusion of the literature. Using the RevMan 5.3 software to conduct the meta-analysis.

Results: This study will compare the effectiveness and safety of traditional Chinese medicine acupoint catgut embedding guided by musculoskeletal ultrasound in the treatment of NRS.

Conclusion: The results of this study will be published in internationally influential academic journals to provide evidence-based medical evidence for the clinical effectiveness and safety of traditional Chinese medicine acupoint catgut embedding in the treatment of NRS.

Ethics And Dissemination: This study does not involve specific patients, and all research data comes from publicly available professional literature, so an ethics committee is not required to conduct an ethical review and approval of the study.

Osf Registration Number: DOI 10.17605/OSF.IO/Q492E.
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http://dx.doi.org/10.1097/MD.0000000000025387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021314PMC
April 2021

[Attention should be paid to the internal fixation of femoral neck fracture].

Authors:
Zhi Liu

Zhongguo Gu Shang 2021 03;34(3):200-2

Department of Orthopedics, the Seventh Medical Center of PLA General Hospital, Beijing 100700, China.

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http://dx.doi.org/10.12200/j.issn.1003-0034.2021.03.002DOI Listing
March 2021

A Novel Normalized Cross-Correlation Speckle-Tracking Ultrasound Algorithm for the Evaluation of Diaphragm Deformation.

Front Med (Lausanne) 2021 12;8:612933. Epub 2021 Mar 12.

Department of Respiratory and Critical Care Medicine, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, China.

To develop a two-dimensional normalized cross-correlation (NCC)-based ultrasonic speckle-tracking algorithm for right diaphragm deformation analysis. Six healthy and eight mechanical ventilation patients were enrolled in this study. Images were acquired by a portable ultrasound system in three sections. DICOM data were processed with NCC to obtain the interframe/cumulative vertical and horizontal displacements, as well as the global strain of the right diaphragm, with continuous tracking and drift correction. The NCC algorithm can track the contraction and relaxation of the right diaphragm by following the respiratory movement continuously. For all three sections, the interframe and accumulated horizontal displacements were both significantly larger than the corresponding vertical displacements (interframe values: 0.031, 0.004, and 0.000; cumulative values: 0.039, 0.001, and <0.0001). For the global strain of the right diaphragm, there was no significant difference between each pair of sections (all > 0.05), regardless of whether the horizontal interval of the initial diaphragm point was 1, 3, 5, or 10 times in the sampling interval. This study developed a novel diaphragm deformation ultrasound imaging method. This method can be used to estimate the diaphragm interframe/accumulated displacement in the horizontal and vertical directions and the global strain on three different imaging planes, and it was found that the strain was not sensitive to the imaging plane.
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http://dx.doi.org/10.3389/fmed.2021.612933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994279PMC
March 2021

Wideband composite planar spiral antenna for generation of tunable angular momentum wave.

Opt Express 2021 Feb;29(3):3754-3763

In this paper, a composite planar spiral antenna consisting of an eight-arm equiangular spiral antenna and eight Archimedean spiral antennas has been designed to radiate electromagnetic wave carrying tunable angular momenta in a wide band. A tunable eight-way Wilkinson power divider network is used to offer three kinds of feeding modes for the equiangular spiral antenna, and thus the composite antenna can radiate the electromagnetic waves with angular momenta of the modes l=1, 2, and 3, respectively. The Archimedean spiral is introduced to improve the gain of the composite antenna in the case of the angular momentum of l=3. By analyzing axis ratio (AR) of the proposed antenna, the generated angular momentum of l=1 is spin angular momentum (SAM), and the angular momenta of both l=2 and 3 include SAM and orbital angular momentum (OAM). Simulated and measured results are given to demonstrate good performance including tunable modes, good purity and wide band.
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http://dx.doi.org/10.1364/OE.414163DOI Listing
February 2021

CGAS is a micronucleophagy receptor for the clearance of micronuclei.

Autophagy 2021 Mar 22:1-17. Epub 2021 Mar 22.

Shanghai TB Key Laboratory, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Micronuclei are constantly considered as a marker of genome instability and very recently found to be a trigger of innate immune responses. An increased frequency of micronuclei is associated with many diseases, but the mechanism underlying the regulation of micronuclei homeostasis remains largely unknown. Here, we report that CGAS (cyclic GMP-AMP synthase), a known regulator of DNA sensing and DNA repair, reduces the abundance of micronuclei under genotoxic stress in an autophagy-dependent manner. CGAS accumulates in the autophagic machinery and directly interacts with MAP1LC3B/LC3B in a manner dependent upon its MAP1LC3-interacting region (LIR). Importantly, the interaction is essential for MAP1LC3 recruitment to micronuclei and subsequent clearance of micronuclei via autophagy (micronucleophagy) in response to genotoxic stress. Moreover, in contrast to its DNA sensing function to activate micronuclei-driven inflammation, CGAS-mediated micronucleophagy blunts the production of cyclic GMP-AMP (cGAMP) induced by genotoxic stress. We therefore conclude that CGAS is a receptor for the selective autophagic clearance of micronuclei and uncovered an unprecedented role of CGAS in micronuclei homeostasis to dampen innate immune surveillance.
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http://dx.doi.org/10.1080/15548627.2021.1899440DOI Listing
March 2021

The Relationship between Erythrocytes and Diabetes Mellitus.

J Diabetes Res 2021 25;2021:6656062. Epub 2021 Feb 25.

Department of Biochemistry and Molecular Biology, Baotou Medical College, Baotou, 014040 Inner Mongolia, China.

High blood glucose level (hyperglycemia) is a leading indicator of diabetes mellitus (DM). Erythrocytes are the most abundant cells in the circulation and the first to perceive changes in plasma composition. Long-lasting hyperglycemia affects the structure and function of erythrocytes. The detection of erythrocyte-related indicators can provide a valuable reference for the prevention, diagnosis, and treatment of DM and its complications. This paper reviews the normal structure and function of erythrocytes, the changes in erythrocytes in patients with diabetes, and the role of erythrocytes in the development of diabetic complications to provide more indicators for the early prevention of DM complications and to monitor the therapeutic effect of DM.
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http://dx.doi.org/10.1155/2021/6656062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935596PMC
February 2021