Publications by authors named "Zhi Li"

2,135 Publications

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Synergistic effect and antibiofilm activity of an antimicrobial peptide with traditional antibiotics against multi-drug resistant bacteria.

Microb Pathog 2021 Jun 18:105056. Epub 2021 Jun 18.

Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao, 266003, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266003, China. Electronic address:

Combined treatment of AMPs with classical antibiotics has gained interest because it often results in a synergistic antibacterial effect. We demonstrated here that Pt5-1c, an AMP derived from phosvitin, had antibacterial activity against the MDR bacteria (S. aureus USA500, E. coli 577 and K. pneumoniae 2182) in the presence of serum. On this basis, we showed that Pt5-1c was synergistically active with traditional antibiotics (oxacillin, vancomycin, streptomycin and azithromycin) against the three MDR bacteria growing as biofilms in vitro and in vivo. Moreover, Pt5-1c restored sensitivity of S. aureus USA500 to oxacillin and vancomycin, E. coli 577 to streptomycin and K. pneumoniae 2182 to azithromycin. Importantly, long-term exposure to Pt5-1c did not give rise to antimicrobial resistance. Collectively, these data not only suggest a promising combinatorial therapy strategy to combat antibiotics-tolerant infections but also present a possibility of Pt5-1c being used to prolong the application of antibiotics including oxacillin, vancomycin, streptomycin and azithromycin, that are under threat of becoming ineffective due to antibiotic resistance.
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http://dx.doi.org/10.1016/j.micpath.2021.105056DOI Listing
June 2021

ceRNA network development and tumor-infiltrating immune cell analysis in hepatocellular carcinoma.

Med Oncol 2021 Jun 19;38(7):85. Epub 2021 Jun 19.

Department of Interventional Radiology, The First Affiliated Hospital of Soochow University, Jiangsu Province, Suzhou, 215006, People's Republic of China.

Hepatocellular carcinoma (HCC) is among the primary causes of cancer deaths globally. Despite efforts to understand liver cancer, its high morbidity and mortality remain high. Herein, we constructed two nomograms based on competing endogenous RNA (ceRNA) networks and invading immune cells to describe the molecular mechanisms along with the clinical prognosis of HCC patients. RNA maps of tumors and normal samples were downloaded from The Cancer Genome Atlas database. HTseq counts and fragments per megapons per thousand bases were read from 421 samples, including 371 tumor samples and 50 normal samples. We established a ceRNA network based on differential gene expression in normal versus tumor subjects. CIBERSORT was employed to differentiate 22 immune cell types according to tumor transcriptomes. Kaplan-Meier along with Cox proportional hazard analyses were employed to determine the prognosis-linked factors. Nomograms were constructed based on prognostic immune cells and ceRNAs. We employed Receiver operating characteristic (ROC) and calibration curve analyses to estimate these nomogram. The difference analysis found 2028 messenger RNAs (mRNAs), 128 micro RNAs (miRNAs), and 136 long non-coding RNAs (lncRNAs) to be significantly differentially expressed in tumor samples relative to normal samples. We set up a ceRNA network containing 21 protein-coding mRNAs, 12 miRNAs, and 3 lncRNAs. In Kaplan-Meier analysis, 21 of the 36 ceRNAs were considered significant. Of the 22 cell types, resting dendritic cell levels were markedly different in tumor samples versus normal controls. Calibration and ROC curve analysis of the ceRNA network, as well as immune infiltration of tumor showed restful accuracy (3-year survival area under curve (AUC): 0.691, 5-year survival AUC: 0.700; 3-year survival AUC: 0.674, 5-year survival AUC: 0.694). Our data suggest that Tregs, CD4 T cells, mast cells, SNHG1, HMMR and hsa-miR-421 are associated with HCC based on ceRNA immune cells co-expression patterns. On the basis of ceRNA network modeling and immune cell infiltration analysis, our study offers an effective bioinformatics strategy for studying HCC molecular mechanisms and prognosis.
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http://dx.doi.org/10.1007/s12032-021-01534-6DOI Listing
June 2021

Comparative Metabolomic and Transcriptomic Studies Reveal Key Metabolism Pathways Contributing to Freezing Tolerance Under Cold Stress in Kiwifruit.

Front Plant Sci 2021 1;12:628969. Epub 2021 Jun 1.

Key Laboratory for Fruit Tree Growth, Development and Quality Control, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China.

Cold stress poses a serious treat to cultivated kiwifruit since this plant generally has a weak ability to tolerate freezing tolerance temperatures. Surprisingly, however, the underlying mechanism of kiwifruit's freezing tolerance remains largely unexplored and unknown, especially regarding the key pathways involved in conferring this key tolerance trait. Here, we studied the metabolome and transcriptome profiles of the freezing-tolerant genotype KL () and freezing-sensitive genotype RB (), to identify the main pathways and important metabolites related to their freezing tolerance. A total of 565 metabolites were detected by a wide-targeting metabolomics method. Under (-25°C) cold stress, KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway annotations showed that the flavonoid metabolic pathways were specifically upregulated in KL, which increased its ability to scavenge for reactive oxygen species (ROS). The transcriptome changes identified in KL were accompanied by the specific upregulation of a codeinone reductase gene, a chalcone isomerase gene, and an anthocyanin 5-aromatic acyltransferase gene. Nucleotides metabolism and phenolic acids metabolism pathways were specifically upregulated in RB, which indicated that RB had a higher energy metabolism and weaker dormancy ability. Since the LPCs (LysoPC), LPEs (LysoPE) and free fatty acids were accumulated simultaneously in both genotypes, these could serve as biomarkers of cold-induced frost damages. These key metabolism components evidently participated in the regulation of freezing tolerance of both kiwifruit genotypes. In conclusion, the results of this study demonstrated the inherent differences in the composition and activity of metabolites between KL and RB under cold stress conditions.
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http://dx.doi.org/10.3389/fpls.2021.628969DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204810PMC
June 2021

[Comparison of plasma levels and immunoactivities of different forms of circulating-free DNA in systemic lupus erythematosus patients].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Jul;37(7):633-640

Department of Laboratory Medicine, First Affiliated Hospital, Wannan Medical College, Wuhu 241001, China. *Corresponding author, E-mail:

Objective To detect and analyze the plasma levels and immunoactivities of different forms of circulating-free DNA (cfDNA) in systemic lupus erythematosus (SLE) patients. Methods The study enrolled 58 patients with SLE, 66 patients with other autoimmune diseases (non-SLE) and 60 healthy individuals. Total cfDNA, exosome cfDNA and immune complex cfDNA were extracted from the plasma and detected using a fluorescence method. Overall methylation levels of cfDNA were measured. Macrophages and dendritic cells induced in vitro were co-cultured with exosomes or immune complexes derived from SLE patients pre-treated with deoxyribonuclease 1-like 3(DNASE1L3) or immunoglobulin G (IgG) specific degradation enzyme or none. Then, cytokines and cell surface activation markers were detected by the multiple fluorescent microsphere assay. Results Among the three groups, SLE patients had the highest levels of exosomes and immune complex cfDNA, followed by non-SLE patients, and no significant differences were found in the simple cfDNA. The methylation levels of different types of cfDNA (except simple cfDNA) in the plasma of SLE patients were significantly lower than that of the non-SLE group and healthy individuals. Non-SLE patients and healthy individuals presented no significant differences for the methylation levels of all cfDNA types. After DNASE1L3 and IgG enzyme treatment, the macrophages and dendritic cells stimulated by exosomes and immune complex cfDNA secreted significantly lower levels of cytokines including MIP-1a, MIP-1b, IL-4, IL-1β, IL-2, IL-1ra, MCP-1, IL-6, IL-9, IL-8, TNF-α and IFN-α. Compared with IgG enzyme treatment, DNASE1L3-treated exosomes and immune complex induced the lower level of cytokines secretion. The expression of CD80, CD86 and CD40 on the surfaces of macrophages and dendritic cells stimulated with exosomes and immune complexes pre-treated with DNASE1L3 enzyme were significantly reduced, but the expression of CD86 on the macrophages was slightly changed. The expression of CD80, CD86 and CD40 showed no difference in the two types of cells stimulated with exosomes and immune complexes pre-treated with IgG enzymes or none, but an overall downward trend existed indeed. Conclusion Exosomes and immune complex cfDNA increase significantly in the plasma of SLE patients, and they can stimulate strong responses of macrophages and dendritic cells.
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July 2021

Recent Progress on Metal-Organic Framework and Its Derivatives as Novel Fire Retardants to Polymeric Materials.

Nanomicro Lett 2020 Aug 25;12(1):173. Epub 2020 Aug 25.

IMDEA Materials Institute, C/Eric Kandel 2, 28906, Getafe, Madrid, Spain.

High flammability of polymers has become a major issue which has restricted its applications. Recently, highly crystalline materials and metal-organic frameworks (MOFs), which consisted of metal ions and organic linkers, have been intensively employed as novel fire retardants (FRs) for a variety of polymers (MOF/polymer). The MOFs possessed abundant transition metal species, fire-retardant elements and potential carbon source accompanied with the facile tuning of the structure and property, making MOF, its derivatives and MOF hybrids promising for fire retardancy research. The recent progress and strategies to prepare MOF-based FRs are emphasized and summarized. The fire retardancy mechanisms of MOF/polymer composites are explained, which may guide the future design for efficient MOF-based FRs. Finally, the challenges and prospects related to different MOF-based FRs are also discussed and aim to provide a fast and holistic overview, which is beneficial for researchers to quickly get up to speed with the latest development in this field.
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http://dx.doi.org/10.1007/s40820-020-00497-zDOI Listing
August 2020

Correction for: Integrated analysis of microRNA and mRNA expression profiling identifies BAIAP3 as a novel target of dysregulated hsa-miR-1972 in age-related white matter lesions.

Aging (Albany NY) 2021 Jun 14;13(11):15688-15689. Epub 2021 Jun 14.

Translational Medicine Research Center (TMRC), School of Pharmaceutical Sciences, Xiamen University, Xiamen, Fujian, China.

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http://dx.doi.org/10.18632/aging.203217DOI Listing
June 2021

Atomically dispersed Fe atoms anchored on COF-derived N-doped carbon nanospheres as efficient multi-functional catalysts.

Chem Sci 2019 Nov 30;11(3):786-790. Epub 2019 Nov 30.

Department of Chemistry, Tsinghua University Beijing 100084 China

Non-noble metal isolated single atom site (ISAS) catalysts have attracted much attention due to their low cost, ultimate atom efficiency and outstanding catalytic performance. Herein, atomically dispersed Fe atoms are prepared by a covalent organic framework (COF)-absorption-pyrolysis strategy. The obtained Fe ISASs anchored on COF-derived N-doped carbon nanospheres (Fe-ISAS/CN) served as a multi-functional catalyst in electro-catalysis and organic catalysis, exhibiting better catalytic performance than commercial Pt/C for the ORR with good stability and methanol tolerance. Besides electro-catalysis, the Fe-ISAS/CN also showed outstanding catalytic performance in organic reactions, such as the selective oxidation of ethylbenzene to acetophenone and dehydrogenation of 1,2,3,4-tetrahydroquinoline with excellent reactivity, selectivity, stability and recyclability. Co and Ni ISAS materials can also be prepared by this method, suggesting that it is a general strategy to obtain metal ISAS catalysts. This work will provide new insight into the design of COF-derived metal ISAS multi-functional catalysts for electro-catalysis and organic reactions using rationally designed synthetic routes and the optimized structure of substrates.
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http://dx.doi.org/10.1039/c9sc05005aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145617PMC
November 2019

Numerical Simulations of the Influence of Inert Gases (N/CO) on Combustion Characteristics of Laminar-Premixed Biosyngas Flame.

ACS Omega 2021 Jun 25;6(22):14585-14597. Epub 2021 May 25.

College of Mechanical and Electronic Engineering, Shandong University of Science and Technology, Qingdao 266590, China.

In this work, the influence of different N/CO contents (up to 60% in fuel volume) on combustion features of laminar-premixed CO/CH/H flame with various equivalence ratios (0.6-1.6) at standard conditions was numerically calculated using ANSYS CHEMKIN-PRO with the GRI-Mech 3.0 mechanism. The mole fraction profiles of the major species and the rate of production of dominant elementary reactions in the flames of CO/CH/H/N/CO/air were obtained. The effect of inert gas addition on the formation of NO , H, O, and OH was analyzed, and the sensitivity coefficient of the active radical mole fraction was obtained. The results suggest that the addition of inert gas of the fuel mixture with various equivalence ratios reduces laminar burning velocity and adiabatic temperature, which have always had a good positive correlation and the maximum peak point shifted left. CO has obvious inhibitory effect on the formation of NO by reducing the amount of O radicals and obstructing the conduct of the reaction of NNH + O ⇔ NH + NO, but it promotes the formation of NO mainly through the reaction HO + NO ⇔ NO + OH. The reactions H + O + HO ⇔ HO + HO, H + O ⇔ O + OH, and OH + CO ⇔ H + CO are three very important reactions for the molar fractions of H, O, and OH that decrease significantly with an increase of inert gas concentration.
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http://dx.doi.org/10.1021/acsomega.1c01729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190912PMC
June 2021

Genotypic Males Play an Important Role in the Creation of Genetic Diversity in Gynogenetic Gibel Carp.

Front Genet 2021 28;12:691923. Epub 2021 May 28.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Hubei Hongshan Laboratory, Chinese Academy of Sciences, Wuhan, China.

Unisexual lineages are commonly considered to be short-lived in the evolutionary process as accumulation of deleterious mutations stated by Muller's ratchet. However, the gynogenetic hexaploid gibel carp () with existence over 0.5 million years has wider ecological distribution and higher genetic diversity than its sexual progenitors, which provides an ideal model to investigate the underlying mechanisms on countering Muller's ratchet in unisexual taxa. Unlike other unisexual lineages, the wild populations of gibel carp contain rare and variable proportions of males (1-26%), which are determined via two strategies including genotypic sex determination and temperature-dependent sex determination. Here, we used a maternal gibel carp from strain F to be mated with a genotypic male from strain A, a temperature-dependent male from strain A, and a male from another species common carp (), respectively. When the maternal individual was mated with the genotypic male, a variant of gynogenesis was initiated, along with male occurrence, accumulation of microchromosomes, and creation of genetic diversity in the offspring. When the maternal individual was mated with the temperature-dependent male and common carp, typical gynogenesis was initiated that all the offspring showed the same genetic information as the maternal individual. Subsequently, we found out that the genotypic male nucleus swelled and contacted with the female nucleus after fertilization although it was extruded from the female nucleus eventually, which might be associated with the genetic variation in the offspring. These results reveal that genotypic males play an important role in the creation of genetic diversity in gynogenetic gibel carp, which provides insights into the evolution of unisexual reproduction.
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http://dx.doi.org/10.3389/fgene.2021.691923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194356PMC
May 2021

Highly selective fluorescence sensor sensing benzo[a]pyrene in water utilizing carbon dots derived from 4-carboxyphenylboronic acid.

Chemosphere 2021 Jun 7;282:131127. Epub 2021 Jun 7.

State Key Laboratory of Heavy Oil Processing, State Key Laboratory of Petroleum Pollution Control, China University of Petroleum-Beijing, Beijing, 102249, China. Electronic address:

4-Carboxyphenylboronic acid was used as the single precursor to facilely prepare fluorescent carbon quantum dots by one-step solvothermal method. The as-obtained carbon dots (CDs) exhibited highly selective and sensitive for benzo[a]pyrene (BaP), and may be a splendid sensor for sensing BaP. The principle was that the as-prepared CDs could form a complex with BaP through hydrophobic interaction which causes the decrease of fluorescence intensity of CDs by static quenching principle. The constructed fluorescent sensor exhibited excellent linearity ranged from 0.002 to 0.06 μg mL and provided a low limit of detection of 0.16 ng mL. The experimental results showed that this fluorescent sensor resulted in simplicity, rapidness, low cost, short analytical time, and high sensitivity and stability. Validation with real water samples endowed the sensor high reliability and feasibility for BaP determination in practical application in various samples.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131127DOI Listing
June 2021

Screening and identification of Theileria annulata subtelomere-encoded variable secreted protein-950454 (SVSP454) interacting proteins from bovine B cells.

Parasit Vectors 2021 Jun 11;14(1):319. Epub 2021 Jun 11.

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, People's Republic of China.

Background: Theileria annulata is a protozoan parasite that can infect and transform bovine B cells, macrophages, and dendritic cells. The mechanism of the transformation is still not well understood, and some parasite molecules have been identified, which contribute to cell proliferation by regulating host signaling pathways. Subtelomeric variable secreted proteins (SVSPs) of Theileria might affect the host cell phenotype, but its function is still not clear. Therefore, in the present study, we explored the interactions of SVSP454 with host cell proteins to investigate the molecular mechanism of T. annulata interaction with host cells.

Methods: The transcription level of an SVSP protein from T. annulata, SVSP454, was analyzed between different life stages and transformed cell passages using qRT-PCR. Then, SVSP454 was used as a bait to screen its interacting proteins from the bovine B cell cDNA library using a yeast two-hybrid (Y2H) system. The potential interacting proteins of host cells with SVSP454 were further identified by using a coimmunoprecipitation (Co-IP) and bimolecular fluorescence complementation (BiFC) assays.

Results: SVSP454 was transcribed in all three life stages of T. annulata but had the highest transcription during the schizont stage. However, the transcription level of SVSP454 continuously decreased as the cultures passaged. Two proteins, Bos Taurus coiled-coil domain 181 (CCDC181) and Bos Taurus mitochondrial ribosomal protein L30 (MRPL30), were screened. The proteins CCDC181 and MRPL30 of the host were further identified to directly interact with SVSP454.

Conclusion: In the present study, SVSP454 was used as a bait plasmid, and its prey proteins CCDC181 and MRPL30 were screened out by using a Y2H system. Then, we demonstrated that SVSP454 directly interacted with both CCDC181 and MRPL30 by Co-IP and BiFC assays. Therefore, we speculate that SVSP454-CCDC181/SVSP454MRPL30 is an interacting axis that regulates the microtubule network and translation process of the host by some vital signaling molecules. Identification of the interaction of SVSP454 with CCDC181 and MRPL30 will help illustrate the transformation mechanisms induced by T. annulata.
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http://dx.doi.org/10.1186/s13071-021-04820-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196448PMC
June 2021

Hydrogeochemistry and health hazards of fluoride-enriched groundwater in the Tarim Basin, China.

Environ Res 2021 Jun 8;200:111476. Epub 2021 Jun 8.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address:

Fluoride (F) enrichment reduces the availability of groundwater resources in the arid region, and it is thus important to investigate the hydrogeochemistry and health hazards of fluoride-enriched groundwater. Seventy-two groundwater samples (20 unconfined samples from the piedmont plain, 22 unconfined samples and 30 shallow confined samples from the alluvial plain) were collected in the Tarim Basin of China to illustrate the geochemical processes driving the F enrichment and the incidence of dental fluorosis. The patterns of average ions contents in groundwater are Na > Ca > Mg > K and SO > Cl > HCO > NO > F. The highest F concentration (average 2.16 mg/L) is observed in unconfined groundwater in the alluvial plain, while the lowest (average 0.63 mg/L) is recorded in unconfined groundwater in the piedmont plain. Approximately 5.0% of unconfined groundwater in the piedmont plain, 90.9% of unconfined groundwater and 33.3% of shallow confined groundwater in the alluvial plain contain F concentrations exceeding 1.0 mg/L (Chinese drinking water standard). Mineral dissolution, cation exchange, and evaporation play a significant role in the formation of solutes in groundwater. High-F groundwater is mostly associated with SO·Cl-Na·Ca, SO·Cl-Na·Mg, and SO·Cl-Na types water. Thermodynamic simulations reveal that the dissolution of F-bearing minerals (e.g., fluorite) significantly controls the F contents in groundwater. High concentrations of F are closely related to high HCO, high Na, high salinity, cation exchange, and evaporation. This demonstrates that high F concentrations are caused by the increase in fluorite solubility due to high ionic strength, Ca consumption and the desorption of F from solid surfaces under alkaline conditions. Mixing with the upper unconfined groundwater plays a vital role in the enrichment of F in shallow confined groundwater in the alluvial plain. The health risk assessment based on Dean's classification indicates that the percentage prevalence of fluorosis for boys aged 6 to 18 is 15.5% for Yecheng (YC), 18.4% for Zepu (ZP), 33.3% for Shache (SC), 29.8% for Maigaiti (MG), and 44.9% for Bachu (BC), while that for girls of the same age is 14.3% for YC, 24.3% for ZP, 42.2% for SC, 41.4% for MG, and 45.3% for BC. For male and female adults aged between 19 and 68, the percentage prevalence of fluorosis is: YC (11.5%, 12.0%), ZP (18.3%, 20.0%), SC (35.4%, 35.0%), MG (32.5%, 39.7%), and BC (42.4%, 44.3%). It is obvious that younger generation, especially girls, suffers from more severe dental fluorosis. This study has implications for the effective management of high-F groundwater in arid regions.
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http://dx.doi.org/10.1016/j.envres.2021.111476DOI Listing
June 2021

Comparison of the sensitivity of Western blotting between PVDF and NC membranes.

Sci Rep 2021 Jun 8;11(1):12022. Epub 2021 Jun 8.

College of Basic Medical Sciences, Dalian Medical University, 9-Western Section, Lvshun South Road, Dalian, 116044, Liaoning, China.

Western blotting (WB) is one of the most widely used techniques to identify proteins as well as post translational modifications of proteins. The selection of electroblotted membrane is one of the key factors affecting the detection sensitivity of the protein which is transferred from gel to membrane in WB. The most common used membranes are polyvinylidene fluoride (PVDF) and nitrocellulose (NC) membranes. Which membrane of these two is more suitable for WB has not been reported so far. Here, by incubating proteins which were transferred to PVDF or NC membranes with a series of antibodies and different types of lectins, we investigated the relationship between the binding ability of these two membranes to proteins or glycoproteins and the molecular weight of the target protein. The antibody re-probed ability of the two membranes was also explored. Moreover, we verified the above results by directly incubating proteins having different molecular weights onto PVDF or NC membranes. Bound proteins were stained with direct blue-71, and the staining intensity was quantitated by scanning and densitometry.
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http://dx.doi.org/10.1038/s41598-021-91521-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187435PMC
June 2021

Current status and future prospects of grapevine anthracnose caused by Elsinoe ampelina: An important disease in humid grape-growing regions.

Mol Plant Pathol 2021 Jun 7. Epub 2021 Jun 7.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China.

Anthracnose, caused by Elsinoe ampelina, is one of the most destructive diseases of grapevines worldwide, especially in humid areas. E. ampelina mainly infects young tissues starting from shoots to berries and affects vine vigour and berry yield. The occurrence and the role of the sexual stage in the disease cycle and the grapevine-E. ampelina interaction remain poorly understood. However, the recent genome sequence data of E. ampelina provides the basis for further studies to understand its evolution, pathogenicity mechanisms, and effector repertoire. New studies on E. ampelina have been conducted in recent years. In this pathogen profile, we present a comprehensive literature review of E. ampelina to summarize the findings on its aetiology, infection mechanisms, genome, pathogenicity, and host resistance.

Taxonomy: Elsinoe ampelina Shear; Kingdom Fungi; Phylum Ascomycota; Subphylum Pezizomycotina; Class Dothideomycetes; Subclass Dothideomycetidae; Order Myriangiales Starbäck; Family Elsinoaceae Höhnel; Genus Elsinoe Racib.

Host Range: E. ampelina only infects Vitis species and hybrids.

Distribution: The grapevine anthracnose is distributed worldwide but is most prevalent in Argentina, Australia, Brazil, Canada, China, India, Japan, Korea, New Zealand, South Africa, Thailand, USA, and Uruguay.

Disease Symptoms: E. ampelina causes slightly abundant depressed spots on young leaves, petioles, stems, tendrils, rachises, and berries. Under severe infection conditions, early defoliation, berry dropping, and delayed berry development and ripening may occur.

Genome: The genomes of two E. ampelina isolates, YL-1 and CECT 20119, are publicly released with 8,057 and 10,207 predicted genes, respectively.
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http://dx.doi.org/10.1111/mpp.13076DOI Listing
June 2021

Efficacy of Chinese Herbal Injections for Elderly Patients With pneumonia-A Bayesian Network Meta-analysis of Randomized Control Trials.

Front Pharmacol 2021 21;12:610745. Epub 2021 May 21.

Department of Maternal and Child Health, School of Public Health, Sun Yat-sen University, Guangzhou, China.

Pneumonia is a prevalent and complicated disease among adults, elderly people in particular, and the debate on the optimal Chinese herbal injections (CHIs) is ongoing. Our objective is to investigate the comparative effectiveness of various CHIs strategies for elderly patients with pneumonia. A comprehensive search strategy was executed to identify relevant randomized controlled trials (RCTs) by browsing through several databases from their inception to first, Feb 2020; All of the direct and indirect evidence included was rated by Network meta-analysis under a Bayesian framework. We ultimately identified 34 eligible randomized controlled trials that involved 3,111 elderly participants and investigated 4 CHIs combined with Western medicine (WM) (Xiyanping injection [XYP]+WM, Yanhuning injection [YHN]+WM, Tanreqing injection [TRQ]+WM, Reduning injection [RDN]+WM), contributing 34 direct comparisons between CHIs. Seen from the outcome of Clinical effective rate and time for defervescence, patients taking medicine added with CHIs [Clinical effective rate, XYP + WM(Odd ratio (OR): 0.74, 95%Credible intervals (CrIs):0.55-0.98), YHN + WM(OR: 0.66, 95%CrI: 0.45-0.95), TRQ + WM(OR: 0.65, 95%CrI: 0.50-0.83), RDN + WM(OR: 0.60, 95%CrI: 0.40-0.89); Time for defervescence, YHN + WM(Mean difference (MD): -2.11, 95%CrI: -3.26 to -0.98), XYP + WM(MD: -2.06, 95%CrI: -3.08 to -1.09), RDN + WM(MD: -1.97, 95%CrI: -3.61 to -0.35), TRQ + WM(MD: -1.69, 95%CrI: -2.27 to -1.04)] showed statistically better effect compared with participants in the Control group (CG) who only took WM. Meanwhile, based on the time for disappearance of cough, 3 out of 4 CHIs [TRQ + WM(MD: -2.56, 95%CrI: -3.38 to -1.54), YHN + WM(MD: -2.36, 95%CrI: -3.86 to -1.00) and XYP + WM(MD: -2.21, 95%CrI: -3.72 to -1.10)] strategies indicated improvement of clinical symptoms. Only XYP + WM(MD -1.78, 95%CrI: -3.29 to -0.27) and TRQ + WM (MD: -1.71, 95%CrI: -2.71 to -0.73) could significantly shorten the time for disappearance of pulmonary rales. According to the statistical effect size (The surface under the cumulative ranking), we found that XYP + WM was presumably to be the preferable treatment for treating elderly patients with pneumonia compared with WM alone in terms of clinical effective rate. Our findings were based on very limited evidence and thus should be interpreted with caution. The application of the findings requires further research.
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http://dx.doi.org/10.3389/fphar.2021.610745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176116PMC
May 2021

COVID-19 Experiences and Social Distancing: Insights From the Theory of Planned Behavior.

Am J Health Promot 2021 Jun 2:8901171211020997. Epub 2021 Jun 2.

Department of Psychiatry, McGill University, Montreal, Quebec, Canada.

Purpose: The objective of this study is to identify the relationship between COVID-19 experiences, perceived COVID-19 behavioral control, social norms and attitudes, and future intention to follow social distancing guidelines.

Design: This is a cross-sectional study.

Setting: Participants responded to an on-line survey in June 2020.

Subjects: The study included 3,183 residents within Quebec, Canada aged 18 and over.

Measures: Measures include perceived COVID-19 related discrimination, fear of COVID-19 infection, prior exposure to COVID-19, and prior social distancing behavior. Participants self-reported attitudes, perceived behavioral control, and perceived norms related to social distancing. Finally, we measured social distancing behavioral intention.

Analysis: We evaluated a theory of planned behavior (TPB) measurement model of social distancing using confirmatory factor analysis (CFA). The association between COVID-19 perceived discrimination, fear of infection, previous social distancing behavior, exposure to COVID-19, TPB constructs and behavioral intentions to social distance were estimated using SEM path analysis.

Results: TPB constructs were positively associated with intention to follow social distancing guidelines. Fear of COVID-19 infection and prior social distancing behavior were positively associated with behavioral intentions. In contrast, perceived discrimination was negatively associated with the outcome. Associations between fear of COVID-19, perceived COVID-19 discrimination and behavioral intentions were partially mediated by constructs of TPB.

Conclusions: COVID-19 prevention efforts designed to emphasize positive attitudes, perceived control, and social norms around social distancing should carefully balance campaigns that heighten fear of infection along with anti- discrimination messaging.
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http://dx.doi.org/10.1177/08901171211020997DOI Listing
June 2021

Calorimetric and Dielectric Investigations of Epoxy-Based Nanocomposites with Halloysite Nanotubes as Nanofillers.

Polymers (Basel) 2021 May 18;13(10). Epub 2021 May 18.

Bundesanstalt für Materialforschung und -prüfung (BAM), Unter den Eichen 87, 12205 Berlin, Germany.

Epoxy nanocomposites are promising materials for industrial applications (i.e., aerospace, marine and automotive industry) due to their extraordinary mechanical and thermal properties. Here, the effect of hollow halloysite nanotubes (HNT) on an epoxy matrix (Ep) was the focus of the study. The structure and molecular mobility of the nanocomposites were investigated using a combination of X-ray scattering, calorimetry (differential (DSC) and fast scanning calorimetry (FSC)) and dielectric spectroscopy. Additionally, the effect of surface modification of HNT (polydopamine (PDA) and Fe(OH) nanodots) was considered. For Ep/HNT, the glass transition temperature () was decreased due to a nanoparticle-related decrease of the crosslinking density. For the modified system, Ep/m-HNT, the surface modification resulted in enhanced filler-matrix interactions leading to higher values than the pure epoxy in some cases. For Ep/m-HNT, the amount of interface formed between the nanoparticles and the matrix ranged from 5% to 15%. Through BDS measurements, localized fluctuations were detected as a β- and γ-relaxation, related to rotational fluctuations of phenyl rings and local reorientations of unreacted components. A combination of calorimetry and dielectric spectroscopy revealed a dynamic and structural heterogeneity of the matrix, as confirmed by two glassy dynamics in both systems, related to regions with different crosslinking densities.
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http://dx.doi.org/10.3390/polym13101634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157573PMC
May 2021

Effect of Carbon Black on Rutting and Fatigue Performance of Asphalt.

Materials (Basel) 2021 May 3;14(9). Epub 2021 May 3.

School of Transportation, Southeast University, Nanjing 211189, China.

As an additive to improve the performance of asphalt binder, tire pyrolysis carbon black is gradually being used in road engineering, but the effect of carbon black (CB) with different particle sizes on asphalt modification remains to be further studied. In this study, three kinds of particle sizes and three kinds of contents of CB were used to modify asphalt, and different tests were conducted to research the high temperature performance and fatigue resistance of carbon black modified asphalt binder. It is found that the addition of CB can enhance the rutting resistance and medium temperature fatigue performance of virgin asphalt binder in general. However, for CB of 270 μm and 2.6 μm, its addition under certain contents lead to the decrease of high temperature performance and fatigue performance of the asphalt binder. For aged asphalt, the addition of CB decreases the rutting resistance and improves the fatigue resistance. The recommended content and particle size of CB are 2% and 2.6 μm. This study refines the complex effects of CB on asphalt properties, providing a reference for determining the size and content of CB in asphalt modification.
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http://dx.doi.org/10.3390/ma14092383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125688PMC
May 2021

Construction and Characterization of an Multi-Gene Deletion Strain and Evaluation of Its Potential as a Live-Attenuated Vaccine in Grass Carp.

Vaccines (Basel) 2021 May 3;9(5). Epub 2021 May 3.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

is an important pathogen that causes motile septicemia (MAS) in the aquaculture industry. Aerolysin, hemolysin, serine protease and enterotoxins are considered to be the major virulence factors of . In this study, we constructed a five-gene (A, , , and ) deletion mutant strain (named five-gene deletion strain, AHFGDS) to observe the biological characteristics and detect its potential as a live-attenuated vaccine candidate. AHFGDS displayed highly attenuated and showed increased susceptibility to fish blood and skin mucus killing, while the wild-type strain ZYAH72 was highly virulent. In zebrafish (), AHFGDS showed a 240-fold higher 50% lethal dose (LD50) than that of the wild-type strain. Immunization with AHFGDS by intracelomic injection or immersion routes both provided grass carp () significant protection against the challenge of the strain ZYAH72 or J-1 and protected the fish organs from serious injury. Further agglutinating antibody titer test supported that AHFGDS could elicit a host-adaptive immune response. These results suggested the potential of AHFGDS to serve as a live-attenuated vaccine to control infection in aquaculture.
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http://dx.doi.org/10.3390/vaccines9050451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147641PMC
May 2021

Cubic Manganese Potassium Hexacyanoferrate Regulated by Controlling of the Water and Defects as a High-Capacity and Stable Cathode Material for Rechargeable Aqueous Zinc-Ion Batteries.

ACS Appl Mater Interfaces 2021 Jun 1;13(23):26924-26935. Epub 2021 Jun 1.

Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Institute of New Energy, Fudan University, Shanghai 200433, China.

Aqueous zinc ion batteries (A-ZIBs) have been used as new alternative batteries for grid-scale electrochemical energy storage because of their low cost and environmental protection. Finding a suitable and economical cathode material, which is needed to achieve high energy density and long cycle stability, is one of the most important and arduous challenges at the present stage. Potassium manganese hexacyanoferrate (KMHCF) is a kind of Prussian blue analogue. It has the advantages of a large 3D frame structure that can accommodate the insertion/extraction of zinc ions, and is nontoxic, safe, and easy to prepare. However, regularly synthesized KMHCF has higher water and crystal defects, which reduce the possibility of zinc ions' insertion/extraction, and subsequently the discharge capacity and cycling stability. In this work, a KMHCF material with less water and low defects was obtained by adding polyvinylpyrrolidone during the synthesis process to control the reaction process. The KMHCF serves as the cathode of A-ZIBs and exhibits an excellent electrochemical performance providing a specific capacity of 140 mA h g for the initial cycle at a current density of 100 mA g (1 C). In particular, it can maintain a reversible capacity of 85 mA h g, even after 400 cycles at 1 C. Moreover, unlike the traditional zinc storage mechanism of A-ZIBs, we found that the KMHCF electrode undergoes a phase transition process when the KMHCF electrode was activated by a small current density, which is attributed to part of the Mn on the lattice site being replaced by Zn, thus forming a new stable phase to participate in the subsequent electrochemical reaction.
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http://dx.doi.org/10.1021/acsami.1c04129DOI Listing
June 2021

Occurrence and risk assessment of anthelmintics in Tuojiang River in Sichuan, China.

Ecotoxicol Environ Saf 2021 Sep 28;220:112360. Epub 2021 May 28.

College of Architecture and Environment, Sichuan University, Chengdu 610065, China.

Recently, emerging pollutants, such as anthelmintics have attracted an increasing attention worldwide due to their extensive use and notable stability. However, the information on anthelmintics in the environment of southwest China is scarce. Thus, the occurrence, ecological risk and exposure evaluation of nineteen anthelmintics in Tuojiang River, which is one of the largest tributaries of Yangtze River, and drinking water source of Sichuan, southwest China, were investigated. The result showed that the detection frequency of anthelmintics was relatively high in Tuojiang River, ranging from 65% to 100% in river water. Among the seven kinds of anthelmintics, benzimidazoles are the primary anthelmintics, with concentrations up to 61.12 ng/L and 596.06 ng/g in water and sediment of the Tuojiang river, respectively. The total concentration of 19 anthelmintics in sediment samples from non-agricultural area was higher than that in agricultural area(p = 0.000 < 0.05). This could be attributed to anthropogenic activities, which lead to greater discharge and accumulation of anthelmintics in residential area along the river. It's worth to mention that the highest total concentrations of anthelmintics (109.28 ng/L) was found at the junction of rivers in R31 site. The results could be ascribed to the complexity of junction of Tuojiang River and Yangtze River, which could influence the distribution of pollutant. Besides, the ecological risk assessment showed that the macrocyclic lactones rather than benzimidazoles had relatively high toxicity to non-target organisms in aquatic environment (p = 0.000 < 0.05), with the highest RQ value of 101 for Daphnia magna, while benzimidazoles had relatively high concentrations. The exposure risk could be ignored for both children and adults because the daily intake of anthelmintics via water ingestion were below 10 ng/kg/d. In addition, strong correlations were found between sucralose and most of the selected anthelmintics in Tuojiang River, indicating that sucralose might be a good tracer to evaluated the source of anthelmintics in surface water. This study provides the levels, risks and even some tracer information of pollutants for better understanding of anthelmintics in southwest China.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112360DOI Listing
September 2021

CBL-interacting protein kinase 31 regulates rice resistance to blast disease by modulating cellular potassium levels.

Biochem Biophys Res Commun 2021 Jul 28;563:23-30. Epub 2021 May 28.

College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address:

Rice blast disease caused by infection with Magnaporthe oryzae, a hemibiotrophic fungal pathogen, significantly reduces the yield production. However, the rice defense mechanism against blast disease remains elusive. To identify the genes involved in the regulation of rice defense to blast disease, dissociation (Ds) transposon tagging mutant lines were analyzed in terms of their response to M. oryzae isolate Guy11. Among them, CBL-interactingprotein kinase31 (CIPK31) mutants were more susceptible than wild-type plants to blast. The CIPK31 transcript was found to be insensitive to Guy11 infection, and the CIPK31-GFP was localized to the cytosol and nucleus. Overexpression of CIPK31 promoted rice defense to blast. Further analysis indicated that CIPK31 interacts with Calcineurin B-like 2 (CBL2) and CBL6 at the plasma membrane, and cbl2 mutants are more susceptible to blast compared with wild-type plants, suggesting that calcium signaling might partially through the CBL2-CIPK31 signaling regulate rice defense. Yeast two-hybrid results showed that AKT1-like (AKT1L), a potential potassium (K) channel protein, interacted with CIPK31, and the K level was significantly lower in the cipk31 mutants than in the wild-type control. In addition, exogenous potassium application increased rice resistance to blast, suggesting that CIPK31 might interact with AKT1L to increase K uptake, thereby promoting resistance to blast. Taken together, the results presented here demonstrate that CBL2-CIPK31-AKT1L is a new signaling pathway that regulates rice defense to blast disease.
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http://dx.doi.org/10.1016/j.bbrc.2021.05.065DOI Listing
July 2021

Fabrication of Hydroxyapatite/Tantalum Composites by Pressureless Sintering in Different Atmosphere.

ACS Omega 2021 May 3;6(19):12831-12840. Epub 2021 May 3.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China.

The effect of sintering atmosphere (air and Ar) and temperature (1100, 1200, 1300 °C) on the microstructure, mechanical properties, and bioactivity of hydroxyapatite/tantalum (HA/Ta) composites were systematically investigated by pressureless sintering of the mixture of HA and Ta powders. It shows that the sintering atmosphere greatly impacts the phase composition and microstructure of the HA/Ta composites. The higher diffusion of atoms promotes shrinkage and causes deeper reaction fusion between the HA matrix and Ta, which improved the interfacial binding of the HA/Ta composites. The refined grain structure and improved interfacial binding obtained within the Ar atmosphere compared to the air atmosphere benefit the mechanical properties. The maximum bending strength and shrinkage observed for the composites sintered at 1300 °C in the Ar atmosphere are 27.24 MPa and 6.65%, respectively. The cell counting kit-8 (CCK-8) method was used to investigate the in vitro cytocompatibility of HA/Ta composites. The results revealed that the HA/Ta composites sintered with different conditions have no cytotoxicity. The simulated body fluid (SBF) soaking results showed that all of the studied composites possess desirable bioactivity, as demonstrated by their ability to form calcium-deficient carbonate apatite layer on the surfaces. For composites sintered at 1300 °C, the surface apatite layer coverage of the composites obtained in the Ar atmosphere was increased by 139.7% than the ones obtained in air, which confirmed an enhanced bioactive mineralization ability. The results indicated that the HA/Ta composites sintered at 1300 °C in Ar possess desirable mechanical properties and bioactivity. This work opens up the new possibility for preparing HA-based composites and is of great value in biomedical applications.
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http://dx.doi.org/10.1021/acsomega.1c01205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154243PMC
May 2021

Analysis of Intestinal Flora and Levels of Epidermal Growth Factor Receptor, Interleukin-32, and Gastrin 17 in Patients with Gastric Cancer via Carbon Nanoparticle Laparoscopy.

Biomed Res Int 2021 12;2021:6697597. Epub 2021 May 12.

Emergency Medicine Center, The First Hospital of Kunming, Kunming, 650021 Yunnan Province, China.

In order to explore the changes of intestinal flora and serum levels of relevant substances in patients with gastric cancer before and after surgery with carbon nanoparticle laparoscopy, a total of 180 patients with early distal gastric cancer who adopted laparoscopic radical gastrectomy for distal gastric cancer in the general surgery department of TCM Hospital of Shi Jia Zhuang City from January 2018 to January 2020 were selected and randomly divided into two groups: traditional laparoscopic operation (control group) and carbon nanoparticle laparoscopic operation (experimental group) were adopted for treatment for the two groups, respectively. Postoperative evaluation included the difference between the two groups in the operative time, the efficiency of intraoperative lymph node dissection, and the number of lymph node detection. The adverse reactions, changes of intestinal flora before and after surgery in the two groups, and the serum levels of epidermal growth factor receptor (EGFR), interleukin-32 (IL-32), and gastrin 17 were evaluated. In the experimental group, the success rate of carbon nanoparticle tracer black staining reached 100%, and the operation time of the experimental group was significantly shorter than that of the control group ( < 0.05). The lymph node detection rate of the experimental group was higher than that of the control group ( < 0.05), but there was no significant difference in the lymph node metastasis rate between the two groups ( > 0.05). The sentinel lymph node sensitivity of the experimental group reached 92.3%, and the specificity, accuracy, and positive and negative prediction rates reached 100%; the experimental group patients were with an obviously higher incidence of level I-II gastrointestinal reaction ( < 0.05). Postoperative increases in Bifidobacteria and Lactobacillus were observed in both groups, while decreases in Enterococcus and Escherichia coli were observed in both groups ( < 0.05). Moreover, the degree of increase and decrease in the experimental group was greater than that in the control group ( < 0.05). The serum levels of EGFR, IL-32, and gastrin 17 in the two groups were significantly lower than those in the control group on 3 d, 7 d, and 15 d after surgery ( < 0.05). In the radical gastrectomy for distal gastric cancer, carbon nanoparticle laparoscopy was not only helpful for the localization of small tumors but also for the thorough dissection of lymph nodes after the surgery, and the postoperative adverse reactions of carbon nanoparticle laparoscopy were also less, which was of great significance for the improvement of intestinal flora and the reduction of serum levels of EGFR, IL-32, and gastrin 17 in gastric cancer patients.
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http://dx.doi.org/10.1155/2021/6697597DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133849PMC
May 2021

Metformin protects against insulin resistance induced by high uric acid in cardiomyocytes via AMPK signalling pathways in vitro and in vivo.

J Cell Mol Med 2021 May 30. Epub 2021 May 30.

Department of Cardiology, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.

High uric acid (HUA) is associated with insulin resistance (IR) in cardiomyocytes. We investigated whether metformin protects against HUA-induced IR in cardiomyocytes. We exposed primary cardiomyocytes to HUA, and cellular glucose uptake was quantified by measuring the uptake of 2-NBDG, a fluorescent glucose analog. Western blot was used to examine the levels of signalling protein. Membrane of glucose transporter type 4 (GLUT4) was analysed by immunofluorescence. We monitored the impact of metformin on HUA-induced IR and in myocardial tissue of an acute hyperuricaemia mouse model established by potassium oxonate treatment. Treatment with metformin protected against HUA-reduced glucose uptake induced by insulin in cardiomyocytes. HUA directly inhibited the phosphorylation of Akt and the translocation of GLUT4 induced by insulin, which was blocked by metformin. Metformin promoted phosphorylation of AMP-activated protein kinase (AMPK) and restored the insulin-stimulated glucose uptake in HUA-induced IR cardiomyocytes. As a result of these effects, in a mouse model of acute hyperuricaemia, metformin improved insulin tolerance and glucose tolerance, accompanied by increased AMPK phosphorylation, Akt phosphorylation and translocation of GLUT4 in myocardial tissues. As expected, AICAR, another AMPK activator, had similar effects to metformin, demonstrating the important role of AMPK activation in protecting against IR induced by HUA in cardiomyocytes. Metformin protects against IR induced by HUA in cardiomyocytes and improves insulin tolerance and glucose tolerance in an acute hyperuricaemic mouse model, along with the activation of AMPK. Consequently, metformin may be an important potential new treatment strategy for hyperuricaemia-related cardiovascular disease.
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http://dx.doi.org/10.1111/jcmm.16677DOI Listing
May 2021

Adhesion-Enhanced Vertically Oriented Graphene on Titanium-Covered Quartz Glass toward High-Stability Light-Dimming-Related Applications.

ACS Nano 2021 May 26. Epub 2021 May 26.

School of Materials Science and Engineering, Peking University, Beijing 100871, P.R. China.

Improving the adhesion property of graphene directly grown on an insulating substrate is essential for promoting the reliability and durability of the related applications. However, effective approaches have rarely been reported, especially for vertically oriented graphene (VG) films grown on insulating templates. To tackle this, we have developed a facile synthetic strategy by introducing an ultrathin (10 nm-thick) titanium (Ti) film on a quartz glass substrate as the adhesion layer, for plasma-enhanced chemical vapor deposition (PECVD) growth of VG films. This synthetic process induces the formation of Ti, oxygen (O), carbon (C)-containing adhesion layer (Ti (O, C)), offering improved interfacial adhesion due to the formation of chemical bonds among Ti and C atoms. Dramatically improved surface and interface stabilities have been achieved, with regard to its counterpart without a Ti adhesion layer. Moreover, we have also realized precise controls of the transparent/conductive property, surface roughness, and hydrophobicity, , by varying the VG film growth time. We have also demonstrated the very intriguing application potentials of the hybrids in light-dimming related fields, that is, electro-heating defogging lenses and neutral density filters toward medical endoscope defogging and camera photography.
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http://dx.doi.org/10.1021/acsnano.1c03063DOI Listing
May 2021

Highly Stereoselective Intramolecular Carbofluorination of Internal -Ynones Promoted by Selectfluor.

Org Lett 2021 Jun 24;23(11):4488-4492. Epub 2021 May 24.

College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, P.R. China.

Herein, we report a metal-free intramolecular carbofluorination protocol for the synthesis of tetrasubstituted monofluoroalkenes from internal -ynones and Selectfluor with both high stereoselectivity and broad functional group tolerance. The chelation between tetrafluoroborate anion and the oxygen present in the aldehyde group rendered the reaction highly stereoselective, with the tetrafluoroborate serving as the direct fluorine source. Therefore, with addition of sodium tetrafluoroborate, Selectfluor could be reused several times without sacrificing reactivity.
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http://dx.doi.org/10.1021/acs.orglett.1c01441DOI Listing
June 2021

Genome-wide long non-coding RNA association study on Han Chinese women identifies lncHSAT164 as a novel susceptibility gene for breast cancer.

Chin Med J (Engl) 2021 Mar 22;134(10):1138-1145. Epub 2021 Mar 22.

Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Key Laboratory of Dermatology, Anhui Medical University, Ministry of Education, Hefei, Anhui 230032, China.

Background: Single-nucleotide polymorphisms (SNPs)-associated genes and long non-coding RNAs (lncRNAs) can contribute to human disease. To comprehensively investigate the contribution of lncRNAs to breast cancer, we performed the first genome-wide lncRNA association study on Han Chinese women.

Methods: We designed an lncRNA array containing >800,000 SNPs, which was incorporated into a 96-array plate by Affymetrix (CapitalBio Technology, China). Subsequently, we performed a two-stage genome-wide lncRNA association study on Han Chinese women covering 11,942 individuals (5634 breast cancer patients and 6308 healthy controls). Additionally, in vitro gain or loss of function strategies were performed to clarify the function of a novel SNP-associated gene.

Results: We identified a novel breast cancer-associated susceptibility SNP, rs11066150 (Pmeta = 2.34 × 10-8), and a previously reported SNP, rs9397435 (Pmeta = 4.32 × 10-38), in Han Chinese women. rs11066150 is located in NONHSAT164009.1 (lncHSAT164), which is highly expressed in breast cancer tissues and cell lines. lncHSAT164 overexpression promoted colony formation, whereas lncHSAT164 knockdown promoted cell apoptosis and reduced colony formation by regulating the cell cycle.

Conclusions: Based on our lncRNA array, we identified a novel breast cancer-associated lncRNA and found that lncHSAT164 may contribute to breast cancer by regulating the cell cycle. These findings suggest a potential therapeutic target in breast cancer.
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http://dx.doi.org/10.1097/CM9.0000000000001429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143754PMC
March 2021

Activation of palindromes on a degradable modular grafting probe enables ultrasensitive detection of microRNAs.

Chem Commun (Camb) 2021 Jun;57(48):5941-5944

College of Chemistry, Fuzhou University, Fuzhou 350002, China. and School of Pharmacy, Anhui Medical University, Hefei 230031, China.

This work describes a single-stranded degradable modular grafting probe for analyzing microRNA-21. In the system, the exonuclease activity of phi29 polymerase restrains the SYBR Green I/ssDNA induced background. The palindrome activation caused remarkable target fluorescence. The detection limit was achieved as 0.26 fM, showing potential in biochemical analysis.
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http://dx.doi.org/10.1039/d1cc01150bDOI Listing
June 2021

Novel Fe(III)-Polybasic acid coordination polymer nanoparticles with targeted retention for photothermal and chemodynamic therapy of tumor.

Eur J Pharm Biopharm 2021 Aug 18;165:174-184. Epub 2021 May 18.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China; Henan Key Laboratory of Targeted Therapy and Diagnosis of Tumor and Major Diseases, Henan Province, Zhengzhou 450001, China. Electronic address:

The development of Fe-coordination polymer-based nanoparticles, with safe and high anti-tumor effects, for the treatment of tumor is facing challenges such as limited resources and poor targeting. In this study, we prepared Fe-polyhydroxy coordination polymer nanoparticles ([email protected]), based on tartaric acid (TA)-Fe(III) coordination polymer as the new photothermal agent, mannose (M) as the target, and bovine serum albumin (BSA) and polyethyleneimine (PEI) as the carrier materials, and investigated them for targeting the multifunctional therapy of tumors. The [email protected] synthesized via a simple coordination of Fe with TA, bovine serum albumin, and polyethyleneimine under ambient conditions exhibited an appropriate size (~125 nm), electrically neutral surfaces, good biocompatibility, and low normal cell toxicity. The [email protected] are the first to exhibit a remarkable photothermal performance. They also showed a pH-sensitive Fenton-like response that was further enhanced via glutathione response. Interestingly, after a single injection, the [email protected] could be retained at the tumor site for 36 h with an effective photothermal dose, which was attributed to the reduced protein adsorption and slow elimination in tumor cells with the aid of M modification and carrier materials, while that for the [email protected] did so for only 2 h. Tumor ablation was demonstrated by in vivo photothermal and chemokinetic therapy using [email protected], and their safety was evident from the weight changes and blood parameters. These results indicated that the [email protected], as new photothermal and CDT agents, have the potential to be used in clinical tumor therapy nanoplatforms.
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http://dx.doi.org/10.1016/j.ejpb.2021.05.012DOI Listing
August 2021