Publications by authors named "Zhi Li"

2,518 Publications

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Molecular identification and efficacy of entomopathogenic fungi isolates against larvae of the Asian corn borer Ostrinia furnacalis (Lepidoptera: Crambidae) in Xinjiang, China.

J Appl Microbiol 2022 Jul 31. Epub 2022 Jul 31.

Institute of Microbiology Applications, Xinjiang Academy of Agricultural Sciences, Ürümqi, China.

Aims: This study aimed to isolate and identify entomopathogenic fungi (EPF) from fungus-infected Ostrinia furnacalis larvae, screen their bio-efficacy against O. furnacalis, and select the most suitable virulent native EPF for biocontrol agent development.

Methods And Results: The occurrence of EPF isolated from various maize production regions in Xinjiang was investigated. Of 13,864 O. furnacalis cadavers surveyed, 536 were selected, and of 136 fungal specimens collected, 14 species were identified. Four fungal isolates were highly pathogenic to O. furnacalis: Aspergillus sp., Lecanicillium attenuatum, Beauveria bassiana and Penicillium polonicum. The Aspergillus sp. was the most abundant (42.25% distribution frequency). Bioassay results revealed that it was as pathogenic as B. bassiana (positive control), with 96.58% lethality against O. furnacalis (LC : 1.40 × 10 conidia ml , LT : 3.41 days). Through morphological examination and rDNA-benA and rDNA-CaM homogeneity analyses, the isolate was identified as Aspergillus nomius.

Conclusions: Four EPF species were highly pathogenic, with A. nomius being the most prevalent in Xinjiang. A. nomius is a potential biocontrol agent.

Significance And Impact Of Study: For sustainable prevention and control of O. furnacalis infestation, identifying biocontrol agents with high virulence against O. furnacalis is crucial. The findings of this study support the development of EPF-based biocontrol approaches.
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http://dx.doi.org/10.1111/jam.15749DOI Listing
July 2022

IFN-I inducible targets ADAR1 isoforms and fine tunes innate immune activation.

Front Immunol 2022 22;13:939907. Epub 2022 Jul 22.

Cytokine Signaling Unit, Department of Immunology, Institut Pasteur, Paris, France.

Regulation of innate immune responses is essential for maintenance of immune homeostasis and development of an appropriate immunity against microbial infection. We show here that , product of the host gene, is induced by type I interferon (IFN-I) in several human non-immune and immune cell types, in particular in primary myeloid cells. Studies in HeLa cells showed that represses both p110 and p150 ADAR1 and reduces constitutive and IFN-induced A-to-I RNA editing. In line with this, activation of innate sensors and expression of IFN-β and the pro-inflammatory IL-6 are promoted. directly targets transcripts by binding to one specific site in the 3'UTR. Moreover, we could show that endogenous is associated with Ago2 and targets in IFN-stimulated cells. Overall, we propose that, by reducing ADAR1, IFN-I-induced contributes to lowering the activation threshold of innate sensors. Our findings provide new insights into the role of , placing it as a potential fine tuner of dsRNA metabolism, cell homeostasis and innate immunity.
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http://dx.doi.org/10.3389/fimmu.2022.939907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354889PMC
August 2022

Intratumoral IL-28B Gene Delivery Elicits Antitumor Effects by Remodeling of the Tumor Microenvironment in H22-Bearing Mice.

J Immunol Res 2022 28;2022:1345971. Epub 2022 Jul 28.

Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.

IL-28B, belonging to type III interferons (IFN-s), exhibits a potent antitumor activity with reduced regulated T cells (Tregs) population, yet the effect of IL-28B on the tumor microenvironment (TME) and if IL-28B can downregulate Tregs directly are still unknown. In this study, we investigated the effects of IL-28B on Tregs in the spleen and TME in H22 tumor-bearing mice and verified the downregulation of IL-28B on Tregs . We found that rAd-mIL-28B significantly inhibited tumor growth and reduced the frequency of splenic CD4Foxp3 T cells. The levels of CXCL13, ICAM-1, MCP-5, and IL-7 in the serum, and the levels of IL-15 and sFasL in the tumor tissue decreased significantly after rAd-mIL-28B treatment relative to rAd-EGFP. Furthermore, the percentage of CD8 cells in the TME was significantly increased in the rAd-mIL-28B group compared with the untreated group. , splenocytes were stimulated with anti-CD3/CD28 and IL-2 in the presence of TGF- with or without IL-28B for three days and followed by flow cytometric, RT-PCR, and IL-10 production analysis. The results showed that IL-28B significantly reduced the proportion of induced Foxp3 cells. It demonstrated that IL-28B may be used as a promising immunotherapy strategy against cancer.
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http://dx.doi.org/10.1155/2022/1345971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352479PMC
August 2022

Immunogenicity and safety of an inactivated SARS-CoV-2 vaccine (Sinopharm BBIBP-CorV) coadministered with quadrivalent split-virion inactivated influenza vaccine and 23-valent pneumococcal polysaccharide vaccine in China: A multicentre, non-inferiority, open-label, randomised, controlled, phase 4 trial.

Vaccine 2022 Jul 29. Epub 2022 Jul 29.

China National Biotec Group Company Limited, Beijing, China; National Engineering Technology Research Center for Combined Vaccines, Wuhan Institute of Biological Products Company Limited, Wuhan, China. Electronic address:

Background: The safety and immunogenicity of the coadministration of an inactivated SARS-CoV-2 vaccine (Sinopharm BBIBP-CorV), quadrivalent split-virion inactivated influenza vaccine (IIV4), and 23-valent pneumococcal polysaccharide vaccine (PPV23) in adults in China is unknown.

Methods: In this open-label, non-inferiority, randomised controlled trial, participants aged ≥ 18 years were recruited from the community. Individuals were eligible if they had no history of SARS-CoV-2 vaccine or any pneumonia vaccine and had not received an influenza vaccine during the 2020-21 influenza season. Eligible participants were randomly assigned (1:1:1), using block randomization stratified, to either: SARS-CoV-2 vaccine and IIV4 followed by SARS-CoV-2 vaccine and PPV23 (SARS-CoV-2 + IIV4/PPV23 group); two doses of SARS-CoV-2 vaccine (SARS-CoV-2 vaccine group); or IIV4 followed by PPV23 (IIV4/PPV23 group). Vaccines were administered 28 days apart, with blood samples taken on day 0 and day 28 before vaccination, and on day 56.

Results: Between March 10 and March 15, 2021, 1152 participants were recruited and randomly assigned to three groups (384 per group). 1132 participants were included in the per-protocol population (375 in the SARS-CoV-2 + IIV4/PPV23 group, 380 in the SARS-CoV-2 vaccine group, and 377 in the IIV4/PPV23 group). The seroconversion rate (100 % vs 100 %) and GMT (159.13 vs 173.20; GMT ratio of 0.92 [95 % CI 0.83 to 1.02]) of SARS-CoV-2 neutralising antibodies in the SARS-CoV-2 + IIV4/PPV23 group was not inferior to those in the SARS-CoV-2 vaccine group. The SARS-CoV-2 + IIV4/PPV23 group was not inferior to the IIV4/PPV23 group in terms of seroconversion rates and GMT of influenza virus antibodies for all strains except for the seroconversion rate for the B/Yamagata strain. The SARS-CoV-2 + IIV4/PPV23 group was not inferior to the IIV4/PPV23 group regarding seroconversion rates and GMC of Streptococcus pneumoniae IgG antibodies specific to all serotypes. All vaccines were well tolerated.

Conclusions: The coadministration of the inactivated SARS-CoV-2 vaccine and IIV4/PPV23 is safe with satisfactory immunogenicity. This study is registered with ClinicalTrials.gov, NCT04790851.
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http://dx.doi.org/10.1016/j.vaccine.2022.07.033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334936PMC
July 2022

Effects of the Separation Distance between Two Triplet States Produced from Intramolecular Singlet Fission on the Two-Electron-Transfer Process.

J Am Chem Soc 2022 Aug 5. Epub 2022 Aug 5.

Shandong Energy Group Co., Ltd., Jinan, Shandong 250014, People's Republic of China.

To harvest two triplet excitons of singlet fission (SF) via a two-electron transfer efficiently, the revelation of the key factors that influence the two-electron-transfer process is necessary. Here, by using steady-state and transient absorption/fluorescence spectroscopy, we investigated the two-electron-transfer process from the two triplet excitons of intramolecular SF (iSF) in a series of tetracene oligomers (dimer, trimer, and tetramer) with 7,7,8,8-tetracyanoquinodimethane (TCNQ) as an electron acceptor in solution. Quantitative two-electron transfer could be conducted for the trimer and tetramer, and the rate for the tetramer is faster than that for the trimer. However, the maximum efficiency of the two-electron transfer in the dimer is relatively low (∼47%). The calculation result of the free energy change (Δ) of the second-electron transfer for these three compounds (-0.024, -0.061, and -0.074 eV for the dimer, trimer, and tetramer, respectively) is consistent with the experimental observation. The much closer Δ value to zero for the dimer should be responsible for its low efficiency of the two-electron transfer. Different Δ values for these three oligomers are attributed to the different Coulomb repulsive energies between the two positive charges generated after the two-electron transfer that is caused by their various intertriplet distances. This result reveals for the first time the important effect of the Coulomb repulsive energy, which depends on the intertriplet distance, on the two-electron transfer process from the two triplet excitons of iSF.
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http://dx.doi.org/10.1021/jacs.2c03550DOI Listing
August 2022

Colloidal Jamming by Interfacial Self-Assembled Polymers: A Robust Route for Ultrahigh Efficient Encapsulation.

Angew Chem Int Ed Engl 2022 Aug 4. Epub 2022 Aug 4.

China Pharmaceutical University, School of Pharmacy, Longmian Avenue No. 639, 211198, Nanjing, CHINA.

The control of cargo phase-transfer is of interest for many applications in science and technology. Here, we report a simple, versatile and robust method to block the phase-transfer of cargo colloids by interfacial self-assembled amphiphilic polymer molecules. After simply increasing the concentration of amphiphilic polymers, the orientation of interfacial polymer molecules changed from flat to upright direction, forming a thick three-dimensional polymer layer at the oil-water surface. Even under fierce external force, this thick interfacial layer robustly jammed the phase-transfer of cargo colloids, resulting in an ultrahigh encapsulation efficiency (up to 97.1%) for proteins and peptides. One single injection of high insulin-loaded microcomposites (58.3 wt%) persisted the blood glucose level within normoglycemic state for 10 days in type 1 diabetic rats. The mass of administrated amphiphilic polymers was 1889 times smaller than that of microcomposites prepared with non-amphiphilic ones.
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http://dx.doi.org/10.1002/anie.202208738DOI Listing
August 2022

Engineering micro oxygen factories to slow tumour progression via hyperoxic microenvironments.

Nat Commun 2022 Aug 2;13(1):4495. Epub 2022 Aug 2.

State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu, 215123, China.

While hypoxia promotes carcinogenesis, tumour aggressiveness, metastasis, and resistance to oncological treatments, the impacts of hyperoxia on tumours are rarely explored because providing a long-lasting oxygen supply in vivo is a major challenge. Herein, we construct micro oxygen factories, namely, photosynthesis microcapsules (PMCs), by encapsulation of acquired cyanobacteria and upconversion nanoparticles in alginate microcapsules. This system enables a long-lasting oxygen supply through the conversion of external radiation into red-wavelength emissions for photosynthesis in cyanobacteria. PMC treatment suppresses the NF-kB pathway, HIF-1α production and cancer cell proliferation. Hyperoxic microenvironment created by an in vivo PMC implant inhibits hepatocarcinoma growth and metastasis and has synergistic effects together with anti-PD-1 in breast cancer. The engineering oxygen factories offer potential for tumour biology studies in hyperoxic microenvironments and inspire the exploration of oncological treatments.
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http://dx.doi.org/10.1038/s41467-022-32066-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345862PMC
August 2022

Integrated strategy of network analysis prediction and experimental validation to elucidate the possible mechanism of compound Turkish gall ointment in treating eczema.

Chin Med 2022 Jul 30;17(1):89. Epub 2022 Jul 30.

Department of Pharmacy, Affiliated Hospital of Traditional Chinese Medicine, Xinjiang Medical University, Xinjiang, Urumqi, 830011, China.

Background: Compound Turkish gall ointment (CTGO) has a long history of being widely used as a folk medicine in Xinjiang for the treatment of eczema. CTGO is currently in the pre-investigational new drug application stage, but its pharmacological mechanisms of action have not yet been clarified.

Methods: First, a sensitive and reliable ultra-high performance liquid chromatography-Q exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry (UHPLC-Q-Orbitrap HRMS) technique was established. Second, an integrative strategy of network analysis and molecular docking based on identified and retrieved ingredients was implemented to investigate the potential targets and pathways involved in the treatment of eczema with CTGO. Finally, Sprague-Dawley (SD) rats with eczema were prepared to verify the predicted results. The skin conditions of the rats were observed, evaluated, and scored. Skin tissues were observed by hematoxylin-eosin (HE) staining, and the levels of serum interferon-γ (IFN-γ) and interleukin-4 (IL-4) were determined by enzyme-linked immunosorbent assay (ELISA). The expression levels of toll-like receptor 4 (TLR4), nuclear factor kappa-B p65 (NF-κB p65), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were detected by real-time quantitative polymerase chain reaction (RT-qPCR).

Results: A total of 29 compounds were identified. We found 38 active components and 58 targets for the treatment of eczema, which included 118 signaling pathways related to inflammation, immunity, and apoptosis. CTGO significantly improved the skin surface and histopathological characteristics of eczema-affected rats, downregulated the expression of IL-4, TLR4, NF-κB (p65), IL-1β, and TNF-α, and upregulated the expression level of IFN-γ.

Conclusion: We predicted and validated our prediction that CTGO may be used to treat eczema by affecting the TLR4/NF-κB signaling pathway, which provides guidance for future experimental studies.
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http://dx.doi.org/10.1186/s13020-022-00643-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338667PMC
July 2022

Advances in Blue Exciplex-Based Organic Light-Emitting Materials and Devices.

Front Chem 2022 12;10:952116. Epub 2022 Jul 12.

College of Optoelectronic Engineering, Chengdu University of Information Technology, Chengdu, China.

Exciplexes possessing thermally activated delayed fluorescence (TADF) characteristics have received much attention in the fields of organic light-emitting materials and devices over the past decade. In general, an exciplex is a physical mixture between a donor (D) with hole transport properties and an acceptor (A) with electron transport characteristics, and the energy difference between the lowest excited singlet state and the lowest excited triplet state is usually fairly small in terms of the long-range charge-transfer process from D to A. In the processes of photoluminescence and electroluminescence, triplet excitons can be converted to singlet excitons through reverse intersystem crossing and then radiate photons to achieve TADF. As a consequence, triplet excitons can be effectively harvested, and the exciton utilization can be significantly enhanced. Up to now, a large number of exciplexes have been developed and applied to organic light-emitting devices. Notably most of them showed green or red emission, while blue exciplexes are relatively few owing to the spectrum characteristics of the large red-shift and broadened emission. In this study, the latest progress of blue exciplex-based organic light-emitting materials and devices is briefly reviewed, and future research is prospected.
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http://dx.doi.org/10.3389/fchem.2022.952116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320026PMC
July 2022

HDAC5 inactivates CYR61-regulated CD31/mTOR axis to prevent the occurrence of preeclampsia.

Cell Tissue Res 2022 Jul 28. Epub 2022 Jul 28.

Department of Obstetrics, Women's Hospital, Medical School of Zhejiang University, No. 1, Xueshi Road, Zhejiang Province, 310006, Hangzhou, People's Republic of China.

Our study was to pinpoint the significance of histone deacetylase 5 (HDAC5) affecting the pathogenesis of preeclampsia (PE) via CD31/mammalian target of rapamycin (mTOR) axis by regulating cysteine-rich angiogenic inducer 61 (CYR61). Expression of HDAC5, CYR61, and CD31/mTOR in placental tissues of patients with PE and trophoblast cells HTR-8/SVneo cells was determined first followed by their interaction analysis. Following different transfection, the significance of HDAC5 in cell functions was assayed in relation to CYR61 and CD31/mTOR. An in vivo PE mouse model was constructed for further validation. The clinical tissue and in vitro cell experimentations discovered that HDAC5 was downregulated in placental tissues of PE patients and trophoblast cells, while CYR61, CD31, mTOR, and p-mTOR displayed upregulation. After overexpression of HDAC5, trophoblast cell functions were enhanced. HDAC5 reduced the acetylation enrichment of H3K27 to inhibit the expression of CYR61. Furthermore, CYR61 promoted the activation of CD31/mTOR axis, thereby inhibiting HTR-8/SVneo cell functions. The in vivo rat model confirmed the above alterations. Taken together, HDAC5 contributes to downregulation of CYR61 through histone deacetylation, inactivating CD31/mTOR axis, which prevents the occurrence and development of PE.
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http://dx.doi.org/10.1007/s00441-022-03652-7DOI Listing
July 2022

Neuroglobin Is Involved in the Hypoxic Stress Response in the Brain.

Biomed Res Int 2022 18;2022:8263373. Epub 2022 Jul 18.

Scientific and Technical Institute of Population and Family Plan, Jingwu Road # 26, Zhengzhou, 450002 Henan, China.

Neuroglobin is an oxygen-binding heme protein expressed predominantly in the brain. Despite many years of research, the exact distribution and expression of neuroglobin in the neocortical development and under mild hypoxia stress still remain unclear. Therefore, we aim to explore the expression of neuroglobin during neocortex expansion and under mild hypoxia stress . We used Kunming mice to examine the expression of Ngb protein during neocortex expansion. In addition, we analyzed the density of Ngb-positive neural stem cells using the Image-Pro PLUS (v.6) computer software program (Media Cybernetics, Inc.). Our data indicated that the density of the neuroglobin-positive neurons in mice cerebral cortex displayed a downward trend after birth compared with high expression of neuroglobin in a prenatal period. Similarly, we identified that neurons were capable of ascending neuroglobin levels in response to mild hypoxic stress compared with the no intervention group. These findings suggest that neuroglobin behaves as a compensatory protein regulating oxygen provision in the process of neocortical development or under physiological hypoxia, further contributing to the discovery of novel therapeutic methods for neurological disorders, which is clinically important.
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http://dx.doi.org/10.1155/2022/8263373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313969PMC
July 2022

Preoperative CECT-based Radiomic Signature for Predicting the Response of Transarterial Chemoembolization (TACE) Therapy in Hepatocellular Carcinoma.

Cardiovasc Intervent Radiol 2022 Jul 27. Epub 2022 Jul 27.

Department of Interventional Radiology, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou, 215006, Jiangsu, China.

Purpose: To evaluate the efficiency of radiomics signatures in predicting the response of transarterial chemoembolization (TACE) therapy based on preoperative contrast-enhanced computed tomography (CECT).

Materials: This study consisted of 111 patients with intermediate-stage hepatocellular carcinoma who underwent CECT at both the arterial phase (AP) and venous phase (VP) before and after TACE. According to mRECIST 1.1, patients were divided into an objective-response group (n = 38) and a non-response group (n = 73). Among them, 79 patients were assigned as the training dataset, and the remaining 32 cases were assigned as the test dataset.

Methods: Radiomics features were extracted from CECT images. Two feature ranking methods and three classifiers were used to find the best single-phase radiomics signatures for both AP and VP on the training set. Meanwhile, multi-phase radiomics signatures were built upon integration of images from two CECT phases by decision-level fusion and feature-level fusion. Finally, multivariable logistic regression was used to develop a nomogram by combining radiomics signatures and clinic-radiologic characteristics. The prediction performance was evaluated by AUC on the test dataset.

Results: The multi-phase radiomics signature (AUC = 0.883) performed better in predicting TACE therapy response compared to the best single-phase radiomics signature (AUC = 0.861). The nomogram (AUC = 0.913) showed better performance than any radiomics signatures.

Conclusion: The radiomics signatures and nomogram were developed and validated for predicting responses to TACE therapy, and the radiomics model may play a positive role in identifying patients who may benefit from TACE therapy in clinical practice.
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http://dx.doi.org/10.1007/s00270-022-03221-zDOI Listing
July 2022

Heterologous Expressed NbSWP12 from Microsporidia Can Bind with Phosphatidylinositol 3-Phosphate and Affect Vesicle Genesis.

J Fungi (Basel) 2022 Jul 23;8(8). Epub 2022 Jul 23.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.

Microsporidia are a big group of single-celled obligate intracellular organisms infecting most animals and some protozoans. These minimalist eukaryotes lack numerous genes in metabolism and vesicle trafficking. Here, we demonstrated that the spore wall protein NbSWP12 of microsporidium belongs to Bin/Amphiphysin/Rvs (BAR) protein family and can specifically bind with phosphatidylinositol 3-phosphate [Ptdlns(3)P]. Since Ptdlns(3)P is involved in endosomal vesicle biogenesis and trafficking, we heterologous expressed NbSWP12 in yeast and proved that NbSWP12 can target the cell membrane and endocytic vesicles. transformed into (a BAR protein of ) deletion mutant rescued the defect phenotype of vesicular traffic. This study identified a BAR protein function in vesicle genesis and sorting and provided clues for further understanding of how microsporidia internalize nutrients and metabolites during proliferation.
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http://dx.doi.org/10.3390/jof8080764DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332396PMC
July 2022

Electrospun Silk Fibroin/Polylactic-co-glycolic Acid/Black Phosphorus Nanosheets Nanofibrous Membrane with Photothermal Therapy Potential for Cancer.

Molecules 2022 Jul 18;27(14). Epub 2022 Jul 18.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.

Photothermal therapy is a promising treating method for cancers since it is safe and easily controllable. Black phosphorus (BP) nanosheets have drawn tremendous attention as a novel biodegradable thermotherapy material, owing to their excellent biocompatibility and photothermal properties. In this study, silk fibroin (SF) was used to exfoliate BP with long-term stability and good solution-processability. Then, the prepared [email protected] was introduced into fibrous membranes by electrospinning, together with SF and polylactic-co-glycolic acid (PLGA). The SF/PLGA/[email protected] membranes had relatively smooth and even fibers and the maximum stress was 2.92 MPa. Most importantly, the SF/PLGA/[email protected] membranes exhibited excellent photothermal properties, which could be controlled by the [email protected] content and near infrared (NIR) light power. The temperature of SF/PLGA/[email protected] composite membrane was increased by 15.26 °C under NIR (808 nm, 2.5 W/cm) irradiation for 10 min. The photothermal property of SF/PLGA/[email protected] membranes significantly killed the HepG2 cancer cells in vitro, indicating its good potential for application in local treatment of cancer.
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http://dx.doi.org/10.3390/molecules27144563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317578PMC
July 2022

induces a muted host response in human blood that blunts the recruitment of neutrophils.

Proc Natl Acad Sci U S A 2022 Aug 26;119(31):e2123017119. Epub 2022 Jul 26.

Department of Microbiology, New York University Grossman School of Medicine, New York, NY 10016.

is an opportunistic pathogen and chief among bloodstream-infecting bacteria. produces an array of human-specific virulence factors that may contribute to immune suppression. Here, we defined the response of primary human phagocytes following infection with using RNA-sequencing (RNA-Seq). We found that the overall transcriptional response to was weak both in the number of genes and in the magnitude of response. Using an ex vivo bacteremia model with fresh human blood, we uncovered that infection with resulted in the down-regulation of genes related to innate immune response and cytokine and chemokine signaling. This muted transcriptional response was conserved across diverse clones but absent in blood exposed to heat-killed or blood infected with the less virulent staphylococcal species . Notably, this signature was also present in patients with bacteremia. We identified the master regulator exoprotein expression (SaeRS) and the SaeRS-regulated pore-forming toxins as key mediators of the transcriptional suppression. The -mediated suppression of chemokine and cytokine transcription was reflected by circulating protein levels in the plasma. Wild-type elicited a soluble milieu that was restrictive in the recruitment of human neutrophils compared with strains lacking . Thus, blunts the inflammatory response resulting in impaired neutrophil recruitment, which could promote the survival of the pathogen during invasive infection.
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http://dx.doi.org/10.1073/pnas.2123017119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351360PMC
August 2022

HPV16 E6-Activated OCT4 Promotes Cervical Cancer Progression by Suppressing p53 Expression Co-Repressor NCOR1.

Front Oncol 2022 7;12:900856. Epub 2022 Jul 7.

Department of Obstetrics and Gynecology, Women's Hospital, Medicine School of Zhejiang University, Hangzhou, China.

Human papillomaviruses (HPV), mainly HPV16 and HPV18, of high-risk classification are involved in cervical cancer carcinogenesis and progression. Octamer-binding transcription factor 4 (OCT4) is a key transcription factor that is increased in various cancer types. Cervical cancer patients with higher levels of OCT4 had worse survival rates. However, the definite mechanisms underlying its function in the development of cervical cancer still remain to be explicated. Here, our study demonstrated that OCT4 expression was slightly increased in cervical cancer tissues than in precancerous ones. However, OCT4 was significantly upregulated in HPV16-positive tissues, in contrast to the expression profiling for p53. Moreover, knockdown of HPV16 E6 in SiHa cells suppressed the expression of OCT4 with impaired activities of cell proliferation, migration, and invasion, while it recovered the expression of p53. Overexpression of OCT4 and p53 exerted opposite roles on cell proliferation, migration, invasion, and colony formation of cervical cancer cells. More importantly, the enforced expression of OCT4 augmented p53-inhibited cell migration, invasion, and colony formation in human cervical cancer by promoting EMT. Finally, we identified that OCT4 could bind to the promoter region to repress p53 expression by recruiting co-repressor NCOR1 using luciferase, ChIP, and co-IP experiments. We further illustrated that OCT4 not only increased the lung metastasis of cervical cancer but also effectively reversed p53-inhibited lung metastasis. In conclusion, our results suggested that HPV16 E6 activated the expression of OCT4 and subsequently crippled the transcription of p53 co-repressor NCOR1, which contributed to cervical cancer progression.
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http://dx.doi.org/10.3389/fonc.2022.900856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302044PMC
July 2022

"Two-in-one" sulfur and nitrogen co-doped fluorescent silicon nanoparticles: Simultaneous as the fluorescent probe and photocatalyst for in-situ real time visual monitoring and degradation of tetracycline antibiotics.

Sci Total Environ 2022 Jul 19;846:157470. Epub 2022 Jul 19.

Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, PR China. Electronic address:

Detection and removal of contaminants are significant for environmental monitoring and remediation. In the present study, "two-in-one" silicon nanoparticles (SiNPs) were designed and prepared to simultaneously act as the fluorescent probe and degradation catalyst to detect and remove tetracycline (TCs) antibiotics. Thiourea and 3-aminopropyltrimethoxysilane were dopant and silicon source to generate fluorescent sulfur and nitrogen co-doped SiNPs (SN-SiNPs). The blue fluorescence of SN-SiNPs was selectively quenched by TCs due to the inner filter effect, whilst accompanied by the newly appeared yellow-green fluorescence resulting from aggregation induced fluorescence emission effect. Based on this phenomenon, SN-SiNPs can be used as fluorescent colorimetric probes for detection of doxycycline, oxytetracycline and tetracycline with limits of detection of 1.8 μg/L, 3.0 μg/L and 4.2 μg/L, respectively; the semi-quantitation can even be visually achieved by naked eyes. Particularly, SN-SiNPs were capable to catalyze the degradation of the three TCs effectively, achieving the removal rates of doxycycline, oxytetracycline and tetracycline of >90 %, >80 % and > 70 % after 240 min exposure to UV light. The catalytic ability of SN-SiNPs was derived from hydroxyl radical (•OH), superoxide radical (•O) and singlet oxygen (O) produced by SN-SiNPs under UV irradiation. Moreover, integrating the fluorescent probe and photocatalyst together, the proposed SN-SiNPs simultaneously realized catalyzing the degradation of the three TCs and in-situ visually monitoring of the degradation process in real time. This study innovatively proposed an integrated probe for the detection and catalytic degradation of TCs, providing a new "two-in-one" strategy for rapid and simple detection and removal of drug pollutants.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157470DOI Listing
July 2022

The correlation analysis of TERT promoter mutations with IDH1/2 mutations and 1p/19q detected in human gliomas.

Medicine (Baltimore) 2022 Jul 22;101(29):e29668. Epub 2022 Jul 22.

Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, People's Republic of China.

Background: To investigate the correlations between mutations in the telomerase reverse transcriptase (TERT) promoter and isocitrate dehydrogenase (IDH) 1 and 2 mutations or 1p/19q deletion in human gliomas.

Methods: TERT promoter gene and IDH gene mutations in 110 glioma specimens were evaluated using first generation Sanger sequencing. The 1p/19q status was determined with fluorescence in situ hybridization. The relationship between TERT promoter mutations and IDH gene mutations as well as 1p/19q deletion was analyzed using the χ2 test and Spearman rank correlation test.

Results: The TERT promoter mutation rate in 110 glioma specimens was 39.09% (43/110), with a rate of 32.56% (14/43) for C228T mutation and 67.44% (29/43) for C250T mutation. The IDH gene mutation rate in all specimens was 31.82% (35/110), with a rate of 52.78% (19/36) in low-grade gliomas and 21.62% (16/74) in high grade gliomas. The 1p/19q deletion rate was 28.18% (31/110) in all specimens. Correlation analysis revealed that TERT promoter mutation was positively correlated with 1p/19q deletion (relative precision (rp) = 0.244, P = .015). In lower-grade glioma with IDH mutation, TERT promoter mutation was positively correlated with 1p/19q deletion (rp = 0.856, P = .000). The prognosis for gliomas with IDH mutation/TERT mutation/1p/19qdeletion was good. Mutation of the TERT promoter was negatively correlated with IDH gene mutation (rp = -0.290, P = .004), except in 10 cases of oligodendroglioma and 1 case of anaplastic oligodendroglioma.

Conclusion: There may be a complex inter-regulatory relationship between the mutations of the TERT promoter and IDH gene as well as 1p/19q abnormalities in human gliomas.
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http://dx.doi.org/10.1097/MD.0000000000029668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302255PMC
July 2022

Perceptual scale expansion: A natural design for improving the precision of motor control.

Q J Exp Psychol (Hove) 2022 Aug 6:17470218221115075. Epub 2022 Aug 6.

Department of Psychology and Behavioral Sciences, Zhejiang University, Hangzhou, P.R. China.

Space perception is systematically biased. Few theories of spatial bias address the possible functional advantages of mechanisms that produce spatial biases. The scale expansion hypothesis proposes that many spatial biases are due to the perceptual expansion of visual angles, which acts somewhat like a natural magnifying glass in vision. The present study examined the idea that visual expansion may improve motor precision (i.e., reduce motor variability) in movements when using closed-loop control but not when using open-loop control. Experiment 1 tested this idea in an online tracking task (closed-loop control), whereas Experiment 2 tested it in a fast-hitting task (open-loop control). The results were consistent with the hypothesis. To rule out the effect of the task difference (i.e., tracking vs fast hitting), Experiment 3 examined the effect of visual expansion on the variability of motor performance in a line-reproduction task. The control type (closed-loop or open-loop) was manipulated by the form of visual feedback (online or offline). The results were again consistent with the present assumption. Taken together, the present data suggest that perceptual expansion in vision improves motor-control precision when using closed-loop control (but not when using open-loop control), which supports the scale expansion hypothesis. In addition, the present findings also improve our understanding of how visual error amplification affects motor control.
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http://dx.doi.org/10.1177/17470218221115075DOI Listing
August 2022

Multi-omics data integration for subtype identification of Chinese lower-grade gliomas: A joint similarity network fusion approach.

Comput Struct Biotechnol J 2022 2;20:3482-3492. Epub 2022 Jul 2.

Department of Statistics and Probability, Michigan State University, East Lansing, MI 48824, USA.

Lower-grade gliomas (LGG), characterized by heterogeneity and invasiveness, originate from the central nervous system. Although studies focusing on molecular subtyping and molecular characteristics have provided novel insights into improving the diagnosis and therapy of LGG, there is an urgent need to identify new molecular subtypes and biomarkers that are promising to improve patient survival outcomes. Here, we proposed a joint similarity network fusion (Joint-SNF) method to integrate different omics data types to construct a fused network using the Joint and Individual Variation Explained (JIVE) technique under the SNF framework. Focusing on the joint network structure, a spectral clustering method was employed to obtain subtypes of patients. Simulation studies show that the proposed Joint-SNF method outperforms the original SNF approach under various simulation scenarios. We further applied the method to a Chinese LGG data set including mRNA expression, DNA methylation and microRNA (miRNA). Three molecular subtypes were identified and showed statistically significant differences in patient survival outcomes. The five-year mortality rates of the three subtypes are 80.8%, 32.1%, and 34.4%, respectively. After adjusting for clinically relevant covariates, the death risk of patients in Cluster 1 was 5.06 times higher than patients in other clusters. The fused network attained by the proposed Joint-SNF method enhances strong similarities, thus greatly improves subtyping performance compared to the original SNF method. The findings in the real application may provide important clues for improving patient survival outcomes and for precision treatment for Chinese LGG patients. An R package to implement the method can be accessed in Github at https://github.com/Sameerer/Joint-SNF.
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http://dx.doi.org/10.1016/j.csbj.2022.06.065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284445PMC
July 2022

Synthesis of nitrogen-rich magnetic hypercrosslinked polymer as robust adsorbent for the detection of neonicotinoids in honey, tomatoes, lettuce and Chinese cabbage.

J Chromatogr A 2022 Aug 9;1677:463326. Epub 2022 Jul 9.

College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China; College of Science, Hebei Agricultural University, Baoding 071001, China. Electronic address:

In this study, a novel nitrogen-rich magnetic hypercrosslinked polymer ([email protected]) was fabricated via a facile one-pot reaction of 1,3,5-triphenylbenzene, 2,4-dichloropyrimidine and phenyl-functionalized magnetic FeO nanoparticles. The [email protected] possessed high specific surface, abundant nanopores and good stability. Especially, [email protected] exhibited excellent adsorption capability with fast adsorption rate to neonicotinoid insecticides. It was applied as a superior magnetic adsorbent for rapid enrichment and isolation of four neonicotinoid insecticides (thiamethoxam, imidacloprid, acetamiprid and thiacloprid) from honey, tomato, lettuce and Chinese cabbage samples prior to high performance liquid chromatography-diode array detection. Under the optimized conditions, the good linearity was obtained in the range of 1-1000 ng g for honey, tomato, lettuce and Chinese cabbage samples, and the detection limits (S/N = 3) were 0.30-0.67 ng g for honey, 1-1.5 ng g for tomato, lettuce and Chinese cabbage. The spiked recoveries were 80.1-111%, and relative standard deviations were less than 10.0%. These results demonstrate that the developed method can be used as a good alternative method for sensitive determination of neonicotinoid insecticides in complex samples.
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http://dx.doi.org/10.1016/j.chroma.2022.463326DOI Listing
August 2022

The effect of apolipoprotein E gene polymorphism and Lp(a) levels on coronary artery disease with atrial fibrillation.

J Int Med Res 2022 Jul;50(7):3000605221109387

Department of Cardiology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.

Objective: To explore the influence of apolipoprotein E () genotypes and blood lipid metabolism on coronary artery disease (CAD) with atrial fibrillation (AF).

Methods: Patients with suspected CAD were consecutively enrolled and divided into groups with or without CAD and/or AF. Blood lipid levels and genotypes were determined and analysed for associations with CAD and AF.

Results: A total of 2048 patients were included (400 patients without CAD or AF [controls], 126 patients without CAD but with AF, 1294 patients with CAD without AF, and 228 patients with CAD and AF). Age and lipoprotein (a) (Lp[a]) levels were significantly higher in patients with CAD and AF versus those with CAD without AF. Among patients with CAD, the E3/E3 genotype and ε3 allele frequencies were significantly lower in patients with AF than in those without AF, and the E4/E4 genotype and ε4 allele frequencies were significantly increased. Multivariate logistic regression revealed that increased Lp(a) levels and age were independent risk factors for AF in patients with CAD.

Conclusion: Among patients with CAD, those with AF had increased age, ε4 frequencies and Lp(a) levels. Age and Lp(a) levels may be independent risk factors for AF in patients with CAD.
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http://dx.doi.org/10.1177/03000605221109387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310063PMC
July 2022

Facile fabrication of hydroxyl-functionalized hypercrosslinked polymer for sensitive determination of chlorophenols.

Food Chem 2022 Jul 14;396:133694. Epub 2022 Jul 14.

College of Science, Hebei Agricultural University, Baoding 071001, China; College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China. Electronic address:

Three hydroxyl-functionalized hypercrosslinked polymers (HCP-POL, HCP-HQ and HCP-PG) were synthesized by Friedel-Crafts reaction. The HCP-HQ displayed the largest surface area and highest adsorption capacity for chlorophenols (CPs). Thus, the HCP-HQ was further modified with magnetism to obtain M-HCP-HQ. An efficient magnetic solid-phase extraction method with M-HCP-HQ as adsorbent was developed for the first time to simultaneously extract four CPs from water and honey samples before analysis by high performance liquid chromatography-diode array detection. Under optimized conditions, the low detection limits (S/N = 3) were obtained to be 0.06-0.10 ng mL for water and 0.80-1.75 ng g for honey. The method recovery was 80.7%-119%, with relative standard deviations below 9.5%. The enrichment factors of the CPs were in the range of 57-220. The extraction mechanism could be attributed to the strong polar interaction, hydrogen bonding and π-π interactions between the M-HCP-HQ and CPs. The M-HCP-HQ based method can be served as a reliable and sensitive tool for detection CPs in water and honey samples.
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http://dx.doi.org/10.1016/j.foodchem.2022.133694DOI Listing
July 2022

Characteristics and Genomic Diversity of Measles Virus From Measles Cases With Known Vaccination Status in Shanghai, China.

Front Med (Lausanne) 2022 30;9:841650. Epub 2022 Jun 30.

Division of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China.

Although the highly effective measles vaccine has dramatically reduced the incidence of measles, measles, and outbreaks continue to occur in individuals who received the measles vaccine because of immunization failure. In this study, patients who have definite records of immunization were enrolled based on measles surveillance in Shanghai, China, from 2009 to 2017, and genomic characteristics regarding viruses retrieved from these cases provided insights into immunization failure. A total of 147 complete genomes of measles virus (MV) were obtained from the laboratory-confirmed cases through Illumina MiSeq. Epidemiological, and genetic characteristics of the MV were focused on information about age, gender, immunization record, variation, and evolution of the whole genome. Furthermore, systematic genomics using phylogeny and selection pressure approaches were analyzed. Our analysis based on the whole genome of 147 isolates revealed 4 clusters: 2 for the genotype H1 (clusters named H1-A, including 73 isolates; H1-B, including 72 isolates) and the other 2 for D8 and B3, respectively. Estimated nucleotide substitution rates of genotype H1 MV derived using genome and individual genes are lower than other genotypes. Our study contributes to global measles epidemiology and proves that whole-genome sequencing was a useful tool for more refined genomic characterization. The conclusion indicates that vaccination may have an effect on virus evolution. However, no major impact was found on the antigenicity in Shanghai isolates.
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http://dx.doi.org/10.3389/fmed.2022.841650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281471PMC
June 2022

Selection of Digestive Tract Reconstruction After Partial Gastric Sparing Surgery in Patients With Adenocarcinoma of the Esophagogastric Junction of cT-T Stage.

Front Surg 2022 30;9:899836. Epub 2022 Jun 30.

The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, China.

Objective: To investigate the appropriate reconstruction method of the digestive tract after partial gastric sparing surgery for adenocarcinoma of the esophagogastric junction of stage cT-T.

Methods: A retrospective analysis of the clinical data of patients with adenocarcinoma of the esophagogastric junction from January 2015 to January 2019 in the General Surgery Department of Zhengzhou University Affiliated Tumor Hospital was performed. Patients with intraoperative double tract anastomosis composed the double tract reconstruction (DTR) group, and patients with intraoperative oesophagogastrostomy with a narrow gastric conduit group composed the oesophagogastrostomy by a narrow gastric conduit (ENGC) group. We analysed and compared the short-term postoperative complications and long-term postoperative nutritional status of the two groups of patients.

Result: There were no statistically significant differences between the two groups of patients in terms of age, sex, preoperative haemoglobin level, albumin level, cT, cN, neoadjuvant therapy or not, pathological type and Siewert type. In terms of BMI and body weight, the ENGC group was higher than the DTR group, but the difference was not statistically significant (= 0.099, = 0.201). There was no significant difference between the two groups of patients in terms of upper resection margin, operation time, blood loss, tumor diameter, pT, pN and postoperative hospital stay. The gastric resection volume of the DTR group was much larger than that of the ENGC group, and there was a significant difference between the two ( = 0.000). The length of the lower resection margin of the DTR group was also significantly greater than that of the ENGC group ( = 0.000). In terms of surgical approach, the proportion of the DTR group with the abdominal approach was significantly higher than that of the ENGC group, and the difference between the two was statistically significant ( = 0.003). The postoperative exhaust time in the ENGC group was significantly shorter than that in the DTR group ( = 0.013). However, there was no statistically significant difference between the two groups in terms of anastomotic leakage, anastomotic bleeding, intestinal obstruction, abdominal infection, pneumonia, pancreatic leakage, lymphatic leakage,death within 30 days after surgery, or overall complications. In terms of anastomotic stenosis, the incidence in the ENGC group was higher than in the DTR group, and the difference was statistically significant ( = 0.001). There was no significant difference in oral PPI, haemoglobin or albumin levels in patients at 3 months, 6 months, or 12 months after surgery. Comparing reflux/heartburn symptoms at 3 months and 6 months after surgery, we found no statistically significant difference between the two, while in terms of reflux/heartburn symptoms at 12 months after surgery, the findings of the ENGC group were higher than those of the DTR group, and the difference was statistically significant ( = 0.045). In terms of poor swallowing, the ENGC group was always higher than the DTR group, and the difference between the two groups was statistically significant ( < 0.05). There was no statistically significant difference in body weight between the two groups at 3 months or 6 months after surgery. At 12 months after surgery, the body weight of the patients in ENGC group was significantly higher than that in the DTR group, and the difference between the two groups was statistically significant ( = 0.039).

Conclusions: For patients with cT2-T3 stage oesophagogastric junction adenocarcinoma with tumours less than 4 cm in diameter, ENGC anastomosis is recommended for patients with a high tumour upper boundary, with obesity, short mesentery, or disordered vascular arch, and for routine patients, DTR anastomosis is recommended.
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http://dx.doi.org/10.3389/fsurg.2022.899836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280352PMC
June 2022

Association of Maternal Mild Hypothyroidism With Offspring Neurodevelopment in TPOAb-Negative Women: A Prospective Cohort Study.

Front Endocrinol (Lausanne) 2022 29;13:884851. Epub 2022 Jun 29.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.

Objectives: Adequate maternal thyroid hormone availability is crucial for fetal neurodevelopment, but the role of maternal mild hypothyroidism is not clear. We aim to investigate the association of maternal mild hypothyroidism with neurodevelopment in infants at 1 year of age among TPOAb-negative women.

Methods: The present study was conducted within the Jiangsu Birth Cohort. A total of 793 mother-infant pairs were eligible for the present study. Maternal thyroid function was assessed by measuring serum thyroid-stimulating hormone, free thyroxine, and thyroid peroxidase antibodies. Neurodevelopment of infants was assessed by using the Bayley Scales of Infant and Toddler Development third edition screening test (Bayley-III screening test).

Results: In the multivariate adjusted linear regression analyses, infants of women with subclinical hypothyroidism and isolated hypothyroxinemia were associated with decreased receptive communication scores ( = -0.68, = 0.034) and decreased gross motor scores ( = -0.83, = 0.008), respectively. Moreover, infants of women with high-normal TSH concentrations (3.0-4.0 mIU/L) and low FT4 concentrations were significantly associated with lower gross motor scores ( = -1.19, = 0.032), while no differences were observed in infants when the mothers had a high-normal TSH concentration and normal FT4 levels.

Conclusions: Maternal subclinical hypothyroidism is associated with decreased receptive communication scores in infants at 1 year of age. In addition, maternal TSH concentration greater than 4.0 mIU/L and maternal isolated hypothyroxinemia are associated with impaired gross motor ability of infants, especially in infants of women with high-normal TSH concentrations (3.0-4.0 mIU/L).
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http://dx.doi.org/10.3389/fendo.2022.884851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278520PMC
June 2022

Diagnostic and Predictive Values of Ferroptosis-Related Genes in Child Sepsis.

Front Immunol 2022 30;13:881914. Epub 2022 Jun 30.

Department of Infectious Disease, Center for Liver Disease, Peking University First Hospital, Beijing, China.

Background: Early diagnosis of sepsis in children was essential to reducing mortality. This study aimed to explore the value of ferroptosis-related genes in children with sepsis.

Methods: We screened the septic children microarray dataset from the GEO database and analyzed the ferroptosis-related differentially expressed genes (DEGs). A functional analysis of ferroptosis-related DEGs was performed. The protein-protein interaction network was used to identify hub genes. We explored the immune landscape of sepsis and controls. The value of hub genes in diagnosing sepsis was tested in the training (GSE26440) and validation sets (GSE13904), and ELISA was used to verify their diagnostic value in children with sepsis in our hospital.

Results: A total of 2,103 DEGs in GSE26440 were obtained, of which ferroptosis-related DEGs were 34. Enrichment analysis showed significant enrichment in the ferroptosis and hypoxia pathways (i.e., HIF-1 pathway). The top three genes (HMOX1, MAPK14, TLR4) were selected as hub genes. Immunological analysis suggested that 10 cell types (i.e., CD8/CD4 T cells) were lower in sepsis. Immune checkpoint-related genes CD274 (PD-L1), HAVCR2 (TIM3), and SIGLEC15 were overexpressed in sepsis. The AUROC for the diagnosis of sepsis for HMOX1 and TLR4 ranged from 0.77 to 0.81, while the AUROC of MAPK14 reached 0.935 and 0.941 in the training and validation sets. Serum ELISA results of HMOX1 and TLR4 showed no significant difference in differentiating sepsis. The AUROC of MAPK14 was 0.877. When the diagnostic threshold was 74.852 ng/ml, the sensitivity and specificity were 0.906 and 0.719, respectively.

Conclusion: Ferroptosis-related gene MAPK14 is of considerable value in the early diagnosis of sepsis in children.
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http://dx.doi.org/10.3389/fimmu.2022.881914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281550PMC
June 2022

Family instability, parenting, and child externalizing problems: Moderation by maternal sympathetic stress reactivity.

Dev Psychopathol 2022 Jul 18:1-13. Epub 2022 Jul 18.

Department of Psychology, University of Rochester, Rochester, NY, USA.

This multi-method longitudinal study evaluated how changes in maternal sensitive parenting may operate as an indirect factor linking family instability and the development of child externalizing problems over time. This study also investigated how mothers' stress reactivity within the sympathetic nervous system (SNS) may moderate the association between family instability and the development of maternal sensitivity. Participants were 235 families with a young child (M = 2.97 years at the first measurement occasion) and these families were followed for two annual measurement occasions. Maternal sensitivity was observed during two discipline tasks (i.e., forbidden toy, discipline discussion tasks), and maternal SNS stress reactivity was indicated by their salivary alpha-amylase (sAA) reactivity to an interpersonal stressor. Findings revealed significant direct effects of family instability and family instability-x-sAA reactivity interaction in association with the change in maternal sensitivity over time. For both tasks, mothers with greater sAA reactivity exhibited stronger associations between family instability and the growth of their sensitivity. Tests of indirect effects indicated that change in maternal sensitivity operated as an indirect factor between family instability-x-sAA reactivity interaction and the change in child externalizing problems. The present findings have important implications for understanding parental and child sequelae associated with unstable family contexts.
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http://dx.doi.org/10.1017/S095457942200058XDOI Listing
July 2022

Low molecular weight fucoidan alleviates cerebrovascular damage by promoting angiogenesis in type 2 diabetes mice.

Int J Biol Macromol 2022 Jul 13;217:345-355. Epub 2022 Jul 13.

CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Pilot Pilot National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address:

Diabetes leading to brain glucose metabolism disorders and cerebrovascular complications. Fucoidan is a kind of sulfated polysaccharides which found in brown algae, has multiply bioactivities and considered to be a promising therapeutic agent. Despite the increasing amount of evidence suggesting the diabetes protective role of fucoidans, the effect of fucoidan on brain abnormalities in type 2 diabetes mellitus patients remains unclear. In this study a low molecular weight fucoidan (LMWF) was obtained from Saccharina japonica and its effect on the cerebrovascular damage in db/db mice was investigated. Results were shown after LMWF treatment, the degree of cerebrovascular damage, the number of apoptotic neuronal cells and the inflammation were all decreased in db/db mice. Moreover, LMWF could up-regulates CD34 and VEGFA expression in db/db mice brain, and the subintestinal vessel angiogenesis in zebrafish was also promoted by LMWF. Moreover, the lumen formation of HUVEC endothelial cells was rescued by LMWF which was destroyed in high glucose treated endothelial cells. Further study found, LMWF alleviates vascular injury by up-regulating the expression level of phosphorylated PI3K and phosphorylated AKT. Our study indicates that LMWF has the potential to develop a cerebrovascular protection agent for type 2 diabetes patients.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.053DOI Listing
July 2022
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