Publications by authors named "Zhenzhen Liu"

341 Publications

Spectroscopic investigation on the binding interactions between graphene quantum dots and carbonic anhydrase.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Sep 10;265:120369. Epub 2021 Sep 10.

State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China. Electronic address:

As a new member of the nanomaterials family, ultrasmall graphene quantum dots (GQDs) have shown broad application prospects in the field of biomedicine, but the analysis of their biological effects at the molecular level is yet limited. Herein, carbonic anhydrase (CA) was selected as a model protein to assess the interactions between GQDs and biomacromolecules. A range of spectroscopic techniques were employed to systematically investigate the binding interactions between GQDs and CA and the catalytic function of CA in the presence of GQDs was evaluated. Experimental results showed that GQDs could quench the intrinsic fluorescence of CA and the concentration dependent quenching efficiency exhibited an obvious deviation from the linear plot, indicating a static binding mode. Further investigation suggested that van der Waal interactions and hydrogen bonding were the main driving forces. Additionally, circular dichroism measurement showed that the binding of GQDs induced slight conformational changes of CA. The catalytic capability assessment proved that these binding interactions resulted in the reduction of the biological functions of CA. This comprehensive study provided important insight into the interaction of GQDs with biomacromolecules, which would be crucial for the further applications of GQDs and other nanomaterials in the biomedical field.
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http://dx.doi.org/10.1016/j.saa.2021.120369DOI Listing
September 2021

Clinical diagnosis and treatment of breast cancer with brain metastases and establishment of a prognostic model: a 10-year, single-center, real-world study of 559 cases.

Ann Transl Med 2021 Aug;9(16):1331

Department of Breast Disease, Henan Breast Cancer Center, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, China.

Background: This study involved a retrospective analysis of 559 metastatic breast cancer (MBC) patients with brain metastasis (BM). We aimed to establish the effectiveness of different preferred treatment methods and factors affecting overall survival following BM diagnosis (BMOS) and explore the feasibility of systemic treatment for MBC patients with BM.

Methods: Univariate and multivariate analyses were used to assess the efficacy of different preferred treatments and other factors associated with BMOS, and a nomogram was then established based on the results of the univariate analysis.

Results: Patients that initially received systemic drug therapy exhibited a clinical benefit rate (CBR) of 43.9% and an intracranial disease control rate (DCR) of 80.6%. The median time between BM diagnosis and the requirement for local intracranial treatment due to worsening disease status was 10.0 months for these patients (95% CI: 7.811-12.189 months). The median follow-up was 28.0 months, and the median BMOS was 16.0 months. Following BM diagnosis, the systemic drug treatment group had a better outcome than the local brain treatment group, with a respective median BMOS of 22.0 and 16.0 months (χ=7.743, P=0.005). At the time of BM diagnosis, the median BMOS for patients without neurological symptoms diagnosed by regular screen was significantly longer than that of patients with neurological symptoms (18.0 13.0 months, respectively; χ=11.371, P=0.001). Based on these analyses, a nomogram was constructed that incorporated disease-free survival (DFS), Karnofsky performance status (KPS), molecular subtype, number of extracranial metastases, BM location, number of BMs, neurological symptoms, and the preferred treatment approach, with a prediction probability (c-index) value of 0.76.

Conclusions: Systemic drug treatment has a beneficial effect on brain lesions, and effective treatment delays the need for local intracranial treatment. Cranial magnetic resonance imaging (MRI) screening can detect asymptomatic BM in MBC patients (particularly those with HER2-positive or triple-negative disease), offering these patients an opportunity to undergo systemic drug therapy, thereby prolonging their survival. To our knowledge, this is a well-fitted nomogram including current treatment and medical examination strategies to predict BMOS probability that offers value as an adjunct for the prognostic evaluation of MBC-BM patients.
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http://dx.doi.org/10.21037/atm-21-3734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422096PMC
August 2021

Four dimensional second-order topological insulator based on a synthetic plasmonic metasurface.

Opt Lett 2021 Sep;46(18):4631-4634

It is possible to explore higher dimensional topological properties in lower dimensional structures by introducing additional synthetic dimensions. In this Letter, we construct a four-dimensional (4D) second-order topological insulator using gradient nanoparticle arrays arranged in a periodic lattice. The nanoparticle array has spatially varying inter-particle distance along and directions, which can be regarded as two synthetic dimensions. Different from higher-order topological insulators in classical wave systems, the higher-order topological states in this 4D system are protected by a pair of first Chern numbers in two-dimensional (2D) subspaces instead of by the quantized 2D Zak phases. It is shown that there exist (4-1)- and (4-2)-dimensional boundary states for both transverse and longitudinal collective resonant modes, which provides new, to the best of our knowledge, mechanisms for light confinement and control in such a plasmonic superlattice.
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http://dx.doi.org/10.1364/OL.433957DOI Listing
September 2021

Clinically significant intraocular lens decentration and tilt in highly myopic eyes: a swept source optical coherence tomography study.

Am J Ophthalmol 2021 Sep 9. Epub 2021 Sep 9.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Purpose: To investigate the occurrence and risk factors of clinically significant intraocular lens (IOL) decentration and tilt in highly myopic eyes using swept-source anterior segment optical coherence tomography (SS-AS-OCT).

Design: Cross-sectional study.

Methods: This study included 334 participants (334 eyes) with high myopia (axial length (AL) ≥26 mm) who underwent phacoemulsification with IOL implantation. Decentration and tilt of IOL were assessed by SS-AS-OCT. Clinically significant IOL decentration and tilt was defined as decentration ≥ 0.4mm and tilt ≥7°. Routine pre- and post-operative examinations were performed, including visual acuity, refraction, biometric measurement using IOL master700, and objective visual quality evaluated by OPD-Scan III.

Results: Among the 334 highly myopic participants, 71 (21.3%) had clinically significant IOL decentration and 26(7.78%) had clinically significant IOL tilt. The proportion of clinically significant IOL decentration (37.1% vs 14.0%, P<0.001) and tilt (16.2% vs 3.90%, P<0.001) in those with AL ≥ 30mm was significantly higher than those of AL< 30 mm. In multivariable logistic regression model, only AL ≥ 30 mm was associated with clinically significant IOL decentration (OR=1.65, P=0.002). AL ≥ 30mm (OR=2.09, P=0.001) was independent risk factors for clinically significant IOL tilt after adjusting for confounders. AL ≥ 30.3 mm could effectively predict IOL decentration ≥ 0.6mm (area under the curve = 0.802).

Conclusions: Participants with AL longer than 30mm have higher risk of clinically significant IOL decentration and tilt, thus cautious should be taken to implant multifocal or toric IOL for these patients.
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http://dx.doi.org/10.1016/j.ajo.2021.08.017DOI Listing
September 2021

Photoinduced transformation of silver ion by molybdenum disulfide nanoflakes at environmentally relevant concentrations attenuates its toxicity to freshwater algae.

J Hazard Mater 2021 08 7;416:126043. Epub 2021 May 7.

School of Environment, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, International Joint Laboratory on Key Techniques in Water Treatment, Henan Normal University, Xinxiang 453007, China.

The transformation of Ag is strongly correlated with its risks in aquatic environment. Considering the wide application of molybdenum disulfide (MoS) and the inevitable release into the environment, the effects of MoS on Ag transformation and toxicity are of great concerns. This study revealed the pH-dependent reduction of Ag (0.5 mM) to Ag nanoparticles (AgNPs) by MoS (50 mg/L) and solar irradiation obviously accelerates the AgNPs formation (2.638 mg/L per day, pH=7.0) compared with dark condition (0.637 mg/L per day), ascribing to the electrons capture from electron-hole pairs of MoS by Ag. Ionic strengths and natural organic matter decreased the AgNPs yield. Metallic 1 T phase of MoS primarily participated in AgNPs formation and was oxidized to soluble ions (MoO) due to the oxygen generation in valance band. The above processes also occurred between Ag and MoS at environmentally relevant concentrations. Further, photoinduced transformation of Ag by MoS (10-100 μg/L) significantly lowered its toxicity to freshwater algae. The AgNPs formation on MoS reduced the bioavailability of Ag to algae, which was the mechanism for attenuated Ag toxicity. The provided data are helpful for better understanding the roles of MoS on the environmental fates and risks of metal ions under natural conditions.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126043DOI Listing
August 2021

Square-root topological state of coupled plasmonic nanoparticles in a decorated Su-Schrieffer-Heeger lattice.

Opt Lett 2021 Sep;46(17):4256-4259

The square-root operation can generate systems with new (to the best of our knowledge) topological phases whose topological properties are inherited from the parent Hamiltonian. In this Letter, we introduce the concept of square-root topology in the two-dimensional (2D) Su-Schrieffer-Heeger (SSH) model and construct a square-root topological square nanoparticle lattice (SRTL) by inserting additional sites into the original 2D SSH model. We find that the topological states in the SRTL are intriguingly different from those in the corresponding SSH model (with on-site potential) due to the change in symmetrical characteristics. Plasmonic nanoparticle arrays are used to demonstrate this by including both nearest-neighbor and next-nearest-neighbor interactions within the dipole approximation. These unique topological states, such as the single corner mode and multiple topological edge modes, enrich the topological features produced by square-root operation and expand the scope to apply such topological features into photonic systems.
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http://dx.doi.org/10.1364/OL.434306DOI Listing
September 2021

Artificial intelligence manages congenital cataract with individualized prediction and telehealth computing.

NPJ Digit Med 2020 Aug 28;3(1):112. Epub 2020 Aug 28.

Department of Ophthalmology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

A challenge of chronic diseases that remains to be solved is how to liberate patients and medical resources from the burdens of long-term monitoring and periodic visits. Precise management based on artificial intelligence (AI) holds great promise; however, a clinical application that fully integrates prediction and telehealth computing has not been achieved, and further efforts are required to validate its real-world benefits. Taking congenital cataract as a representative, we used Bayesian and deep-learning algorithms to create CC-Guardian, an AI agent that incorporates individualized prediction and scheduling, and intelligent telehealth follow-up computing. Our agent exhibits high sensitivity and specificity in both internal and multi-resource validation. We integrate our agent with a web-based smartphone app and prototype a prediction-telehealth cloud platform to support our intelligent follow-up system. We then conduct a retrospective self-controlled test validating that our system not only accurately detects and addresses complications at earlier stages, but also reduces the socioeconomic burdens compared to conventional methods. This study represents a pioneering step in applying AI to achieve real medical benefits and demonstrates a novel strategy for the effective management of chronic diseases.
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http://dx.doi.org/10.1038/s41746-020-00319-xDOI Listing
August 2020

Essential amino acid metabolism-related molecular classification in triple-negative breast cancer.

Epigenomics 2021 Aug 27;13(16):1247-1268. Epub 2021 Aug 27.

Department of Breast, Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, 450000, China.

To develop an approach to characterize and classify triple-negative breast cancer (TNBC) tumors based upon their essential amino acid (EAA) metabolic activity. We performed bioinformatic analyses of genomic, transcriptomic and clinical data in an integrated cohort of 740 TNBC patients from public databases. Based on EAA metabolism-related gene expression patterns, two TNBC subtypes were identified with distinct prognoses and genomic alterations. Patients exhibiting an upregulated EAA metabolism phenotype were more prone to chemoresistance but also expressed higher levels of immune checkpoint genes and may be better candidates for immune checkpoint inhibitor therapy. Metabolic classification based upon EAA profiles offers a novel biological insight into previously established TNBC subtypes and advances current understanding of TNBC's metabolic heterogeneity.
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http://dx.doi.org/10.2217/epi-2021-0210DOI Listing
August 2021

Evaluation of whether adjuvant chemotherapy can be safely omitted for older female patients with ER-positive, HER2-negative N1 breast cancer: a study based on the SEER database.

Ann Transl Med 2021 Jul;9(13):1082

Department of Breast Disease, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, China.

Background: This study evaluated the trends and practice patterns associated with adjuvant chemotherapy (CT) use for patients aged ≥70 years with estrogen receptor-positive (ER+), human epidermal growth factor receptor 2-negative (HER2-) N1 (1-3 positive lymph nodes) breast cancer (BC). Furthermore, the relationship between adjuvant CT and survival in this set of patients was determined.

Methods: The Surveillance, Epidemiology, and End Results (SEER) database was used to identify 6,711 women with ER+, HER2- N1 BC who were aged ≥70 years between 2010 and 2015. Demographic, clinical, and pathological predictors of CT use were identified using logistic regression. Multivariable Cox regression was used to identify variables that correlated with overall survival (OS), before and after propensity score matching (PSM).

Results: Younger age at diagnosis, other histological types, higher tumor grade, larger tumor size, breast reconstruction surgery, progesterone receptor-negative (PR-), and increased nodal involvement were associated with an increased probability of receiving CT. CT use was associated with improved 5-year OS, both before and after PSM [hazard ratio (HR): 0.66, 95% confidence interval (CI): 0.58-0.75 and HR: 0.81, 95% CI: 0.68-0.96, respectively]. The exploratory subgroup analysis showed that although the benefit of CT was significant in the grade III subgroup, it was not significant in the grades I-II subgroups.

Conclusions: Adjuvant CT improved 5-year OS in patients with ER+, HER2- N1 BC who were aged ≥70 years; however, the benefit of CT was more significant in the grade III subgroup than in the grades I-II subgroups.
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http://dx.doi.org/10.21037/atm-21-3097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339850PMC
July 2021

Findings from the 45 and Up Study: smoking is not associated with the risk of early-onset cataract.

Ann Transl Med 2021 Jul;9(13):1077

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Background: To determine if tobacco smoking is a risk factor for early-onset cataracts.

Methods: This was a prospective population-based cohort study. A total of 70,886 participants aged 45-55 years in the 45 and Up Study were included in our analysis. Early-onset cataracts (EOC) were defined as cataract surgeries performed before 65 years old, based on participant data linked to the Medicare Benefits Schedule (MBS). Smoking habits were assessed at baseline, based on a self-administered questionnaire. A Cox proportional hazards model was used to evaluate the association between tobacco smoking and the risk of early-onset cataracts over the follow-up period.

Results: At baseline recruitment, 59.9% of study participants never smoked, 30.5% were former smokers, and 9.6% were current smokers. A total of 1,713 participants underwent cataract surgery over a mean follow-up of 625,042 person-years, with an incidence of 2.74 cases per 1,000 person-years. For current smokers, patients with EOC had longer smoking durations (P=0.019). For former smokers, patients with EOC had higher smoking intensities (P=0.001), were older at smoking commencement (P=0.011), and longer times since quitting (P=0.04). The risk of EOC was not found to be significantly different between current smokers or former smokers, compared to those who had never smoked. Both stratification and sensitivity analyses by gender, surgery year, alcohol intake, physical activity, and income yielded similar results.

Conclusions: Smoking has neither a beneficial nor harmful effect on the long-term incidence of EOC.
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http://dx.doi.org/10.21037/atm-21-742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339842PMC
July 2021

5-Aminosalicylic Acid Prevents Disease Behavior Progression and Intestinal Resection in Colonic and Ileocolonic Crohn's Disease Patients: A Retrospective Study.

Can J Gastroenterol Hepatol 2021 9;2021:1412663. Epub 2021 Aug 9.

State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, Shaanxi Province, China.

Background And Aims: The efficacy of 5-aminosalicylic acid (5-ASA) in the long-term outcome of Crohn's disease (CD) patients was uncertain. This study aimed to evaluate the efficacy of the 5-ASA in preventing disease behavior progression and intestinal resection in CD patients.

Methods: CD patients were prospectively enrolled from January 2008 to September 2019 in Xijing Hospital. Disease behavior progression was defined as the development of stricturing (B2) or penetrating disease (B3) in patients with nonstricturing/nonpenetrating disease (B1) at diagnosis. Cox regression analyses were used to investigate the associations between disease location progression, disease behavior progression, and intestinal resection and multiple covariates.

Results: In total, 122 CD patients were followed up for 4.3 years. At the time of diagnosis, disease location was ileal in 19.7% (24/122), colonic in 41.0% (50/122), and ileocolonic in 39.3% (48/122). A total of 87 (71.3%) patients had B1 at diagnosis. The disease behavior progression and intestinal resection rates were 42.5% (37/87) and 29.5% (36/122). The use of 5-ASA reduced the risk of disease behavior progression (HR 0.30, 95% CI 0.14-0.61,  = 0.001) and intestinal resection (HR 0.33, 95% CI 0.17-0.90,  = 0.027) in colonic and ileocolonic CD patients. Patients who presented with ileal disease at diagnosis did not have the same protective effects when taking 5-ASA ( > 0.05).

Conclusions: The use of 5-ASA could improve the long-term outcome of CD patients with colon involvement. The result emphasized the importance of early use of 5-ASA in the daily management of colonic involved CD.
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http://dx.doi.org/10.1155/2021/1412663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371663PMC
August 2021

Characteristics of Corneal Higher-Order Aberrations in Congenital Ectopia Lentis Patients.

Transl Vis Sci Technol 2021 Aug;10(9):24

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Purpose: The purpose of this study was to investigate the characteristics of corneal higher-order aberrations (HOAs) in patients with congenital ectopia lentis (CEL).

Methods: Clinical characteristics and HOAs of 60 patients with CEL and 75 healthy controls at Zhongshan Ophthalmic Center in China were retrospectively analyzed. The Q value and the corneal HOAs in the CEL group and the controls were measured by using Pentacam and compared value between the CEL and control groups. The correlation between HOAs and age was investigated using the Pearson correlation analysis.

Results: The Q value of anterior corneal surface in the CEL group was larger than that in the controls (-0.41 ± 0.17 vs. -0.32 ± 0.13, P = 0.001); the total corneal horizontal coma in the CEL group were larger than that in the controls (0.24 ± 0.18 vs. -0.05 ± 0.14, P < 0.001); both the primary spherical aberrations of the anterior and total corneal surface were lower in the CEL group than that in the controls (for anterior corneal surface: 0.15 ± 0.08 vs. 0.27 ± 0.08 µm, P < 0.001; for total corneal surface: 0.10 ± 0.09 vs. 0.23 ± 0.09 µm, P < 0.001), the anterior and total corneal horizontal coma were negatively associated with age, whereas the anterior and total corneal spherical aberrations were positively associated with age in patients with CEL.

Conclusions: Patients with CEL had higher corneal horizontal coma and lower corneal vertical coma primary spherical aberrations than healthy controls.

Translational Relevance: These findings are informative for the clinical managements in patients with CEL.
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http://dx.doi.org/10.1167/tvst.10.9.24DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383910PMC
August 2021

Association of miRNA-146a Gene Polymorphism Rs2910164 with Behcet's Disease: A Meta-analysis.

Ocul Immunol Inflamm 2021 Aug 17:1-7. Epub 2021 Aug 17.

Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Henan Province Eye Hospital, Henan International Joint Research Laboratory for Ocular Immunology and Retinal Injury Repair, Zhengzhou, P.R. China.

Background: A meta-analysis was performed by summarizing relevant researches to evaluate the relationship between miRNA-146a gene polymorphism rs2910164 and Behcet's disease (BD).

Methods: A systematic search of published studies was conducted in PubMed and Embase. Five eligible studies involving 1167 BD cases and 1662 controls were included in the current meta-analysis.

Results: The results suggested that the polymorphism rs2910164 was correlated with BD susceptibility in all genetic models. In subgroup analysis according to ethnicity, the relationship between this polymorphism and BD was more significant in Caucasians (allele model: OR = 0.49, 95% CI 0.39-0.61; heterozygote model: OR = 0.35, 95% CI 0.26-0.47; homozygote model: OR = 0.29, 95% CI 0.16-0.53; dominant model: OR = 0.34, 95% CI 0.25-0.45; recessive model: OR = 0.56, 95% CI 0.50-0.72) than that in Asians.

Conclusion: In conclusion, this meta-analysis indicates that miRNA-146a gene polymorphism rs2910164 G allele confers BD susceptibility, especially in Caucasians.
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http://dx.doi.org/10.1080/09273948.2021.1968002DOI Listing
August 2021

Genetic associations of anti-vascular endothelial growth factor therapy response in age-related macular degeneration: a systematic review and meta-analysis.

Acta Ophthalmol 2021 Aug 17. Epub 2021 Aug 17.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, China.

Purpose: To investigate the association of all reported common polymorphisms in anti-vascular endothelial growth factor (VEGF) therapy response and to identify potential clinically useful biomarkers for anti-VEGF therapy response in patients with age-related macular degeneration (AMD).

Methods: We searched the Embase, PubMed, Web of Science databases in English and the China National Knowledge Infrastructure, WanFang and VIP databases in Chinese for pharmacogenetics studies on anti-VEGF therapy response in AMD. Odds ratios with 95% confidence intervals were calculated using the random effects model.

Results: Among the 10 468 records yielded by the literature search, 33 articles that met the eligibility criteria were included in the meta-analysis. Nine single-nucleotide polymorphisms (SNP) in four genes were observed to be associated with the anti-VEGF therapy response in AMD patients. That is, rs1120063 in the HTRA1 gene; rs10490924 in the age-related maculopathy susceptibility (ARMS2) gene; rs1061170 in the complement factor H (CFH) gene; and rs323085 in the OR52B4 gene were associated with good anti-VEGF therapy responses, while rs800292, rs1410996 and rs1329428 in the CFH gene and rs4910623 and rs10158937 in the OR52B4 gene were associated with poor anti-VEGF therapy response in the AMD patients in our sample.

Conclusion: In this study, nine SNPs of four genes were indicated to be significantly associated with the anti-VEGF therapy response in the samples: rs11200638 in the HTRA1 gene; rs10490924 in the ARMS2 gene; rs1061170, rs800292, rs1410996 and rs1329428 in the CFH gene; and rs323085, rs4910623 and rs10158937 in the OR52B4 gene. Further studies based on various ethnicities and large sample sizes are warranted to strengthen the evidence found in the present study.
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http://dx.doi.org/10.1111/aos.14970DOI Listing
August 2021

[Retracted] MicroRNA‑145 inhibits migration and invasion via inhibition of fascin 1 protein expression in non‑small‑cell lung cancer cells.

Mol Med Rep 2021 Oct 13;24(4). Epub 2021 Aug 13.

Department of Oncology, Xiangya Hospital of Central South University,Changsha, Hunan 410008, P.R. China.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that certain of the Transwell cell migration data shown in Fig. 4 were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to , the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive any reply. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in 12: 6193‑6198, 2015; DOI: 10.3892/mmr.2015.4163].
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http://dx.doi.org/10.3892/mmr.2021.12365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383049PMC
October 2021

Long non-coding RNA profile study identifies a metabolism-related signature for colorectal cancer.

Mol Med 2021 08 3;27(1):83. Epub 2021 Aug 3.

Department of General Surgery, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, China.

Background: Heterogeneity in colorectal cancer (CRC) patients provides novel strategies in clinical decision-making. Identifying distinctive subgroups in patients can improve the screening of CRC and reduce the cost of tests. Metabolism-related long non-coding RNA (lncRNA) can help detection of tumorigenesis and development for CRC patients.

Methods: RNA sequencing and clinical data of CRC patients which extracted and integrated from public databases including The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) were set as training cohort and validation cohort. Metabolism-related genes were acquired from Kyoto Encyclopedia of Genes and Genomes (KEGG) and the metabolism-related lncRNAs were filtered using correlation analysis. The risk score was calculated based on lncRNAs with prognostic value and verified through survival curve, receiver operating characteristic (ROC) curve and risk curve. Prognostic factors of CRC patients were also analyzed. Nomogram was constructed based on the results of cox regression analyses. The different immune status was observed in the single sample Gene Set Enrichment Analysis (ssGSEA).

Results: The training cohort and the validation cohort enrolled 432 and 547 CRC patients respectively. A total of 23 metabolism-related lncRNAs with prognostic value were screened out and 10 of which were significantly differentially expressed between tumour and normal tissues. Finally, 8 lncRNAs were used to establish a risk score (DICER1-AS1, PCAT6, GAS5, PRR7-AS1, MCM3AP-AS1, GAS6-AS1, LINC01082 and ADIRF-AS1). Patients were divided into high-risk and low-risk groups according to the median of risk scores in training cohort and the survival curves indicated that the survival prognosis was significantly different. The area under curve (AUC) of the ROC curve in two cohorts were both greater than 0.6. The age, tumour stage and risk score were selected as independent factors and used to construct a nomogram to predict CRC patients' survival rate with the c-index of 0.806. The ssGSEA indicated that the risk score was associated with immune cells and functions.

Conclusions: Our systematic study established a metabolism-related lncRNA signature to predict outcomes of CRC patients which may contribute to individual prevention and treatment.
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http://dx.doi.org/10.1186/s10020-021-00343-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336290PMC
August 2021

Rhubarb granule promotes diethylnitrosamine-induced liver tumorigenesis by activating the oxidative branch of pentose phosphate pathway via G6PD in rats.

J Ethnopharmacol 2021 Jul 31;281:114479. Epub 2021 Jul 31.

Department of Biochemistry and Molecular Biology, School of Integrated Chinese and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei, Anhui Province, PR China. Electronic address:

Ethnopharmacological Relevance: Rhubarb is a natural herbal medicine widely used clinically with numerous pharmacological activities including anti-cancer. Specifically, several studies reported that free anthraquinones from Rhubarb suppressed the proliferation of hepatoma cells. Nonetheless, recent studies revealed that Rhubarb caused hepatotoxicity in vivo, confirming its "two-way" effect on the liver. Therefore, the efficacy and safety of Rhubarb in the in vivo treatment of liver cancer should be further elucidated.

Aim Of The Study: This study investigated the presence of hepatoprotection or hepatotoxicity of Rhubarb in diethylnitrosamine (DEN)-induced hepatocarcinogenesis.

Material And Methods: A total of 112 male Sprague-Dawley rats weighing 190-250 g were enrolled. The rats were induced hepatocarcinogenesis using diethylnitrosamine (0.002 g/rat) until 17 weeks. Starting at week 11, Rhubarb granules (4 g/kg and 8 g/kg) were intragastrically administered daily for 7 weeks. All rats were euthanized at week 20 and the livers were analyzed via non-targeted metabolomics analysis. We established hepatic glucose 6 phosphate (6PG) levels and glucose 6 phosphate dehydrogenase (G6PD) activities to assess the pentose phosphate pathway (PPP). And the liver injuries of rats were analyzed via histological changes, hepatic function, as well as hepatic protein levels of alpha-fetoprotein (AFP), pyruvate kinase isozyme type M2 (PKM2), and proliferating cell nuclear antigen (PCNA). Furthermore, polydatin (0.1 g/kg/d) as a specific inhibitor of G6PD was used to treat rats. Notably, their histological changes, hepatic function, hepatic 6PG levels, hepatic G6PD activities, PCNA levels, and PKM2 levels were recorded.

Results: Non-targeted metabolomics revealed that Rhubarb regulated the PPP in the liver of Rhubarb-DEN-treated rats. Besides, Rhubarb activated the oxidative branch of the PPP by activating G6PD (a rate-limiting enzyme in the oxidative PPP) in the liver of Rhubarb-DEN-treated rats. Meanwhile, Rhubarb promoted DEN-induced hepatocarcinogenesis. Moreover, polydatin attenuated the promoting effect of Rhubarb on DEN-induced hepatocarcinogenesis.

Conclusions: Rhubarb promoted DEN-induced hepatocarcinogenesis by activating the PPP, indicating that the efficacy and safety of Rhubarb in the treatment of liver cancer deserve to be deliberated.
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http://dx.doi.org/10.1016/j.jep.2021.114479DOI Listing
July 2021

Prevalence and time trends of refractive error in Chinese children: A systematic review and meta-analysis.

J Glob Health 2021 17;11:08006. Epub 2021 Jul 17.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, China.

Background: To investigate the prevalence and time trends of refractive error (RE) among Chinese children under 18 years old.

Methods: PubMed, Embase, Web of Science were searched for articles that estimated prevalence of RE in Chinese children. Data of identified eligible studies was extracted by two investigators independently. Pooled prevalence of RE and its 95% confidence interval (95% CI) and the time trends of RE were investigated using Meta-analysis methods.

Results: Of the 41 studies covering 1 051 784 subjects, the pooled prevalence of myopia, high myopia, hyperopia and astigmatism in Chinese children was 38.0% (95% confidence interval (CI) = 35.1%-41.1%), 2.8% (95% CI = 2.3%-3.4%), 5.2% (95% CI = 3.1%-8.6%) and 16.5% (95% CI = 12.3%-21.8%), respectively. Subgroup analysis show that children living in urban were at higher risk of RE. Prevalence of myopia and hyperopia were higher in Northern China compared with Southern China and high myopia and astigmatism were higher in Hong Kong, Macau and Taiwan than in mainland China. Regression analysis showed an upward trend in myopia and hyperopia and a downward trend in high myopia and astigmatism with years.

Conclusions: The prevalence of RE is higher in urban areas than in rural for Chinese children. The much higher prevalence of myopia and astigmatism in China compared with foreign countries indicates the important role played by environment and genetic factors. Considering the large magnitude of refractive errors, much more attention should still be paid to RE prevention and treatment strategy development in China.
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http://dx.doi.org/10.7189/jogh.11.08006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285767PMC
August 2021

Sulfur vacancies affect the environmental fate, corona formation, and microalgae toxicity of molybdenum disulfide nanoflakes.

J Hazard Mater 2021 10 25;419:126499. Epub 2021 Jun 25.

School of Environment, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, International Joint Laboratory on Key Techniques in Water Treatment, Henan Normal University, Xinxiang 453007, China.

Sulfur vacancy (SV) defects have been engineered in two-dimensional (2D) transition metal dichalcogenides (TMDs) for high performance applications in various fields involving environmental protection. Understanding the influence of SVs on the environmental fate and toxicity of TMDs is critical for evaluating their risk. Our work discovered that SVs (with S/Mo ratios of 1.65 and 1.32) reduced the dispersibility and promoted aggregation of 2H phase molybdenum disulfide (2H-MoS, a hot TMD) in aqueous solution. The generation capability of •O and •OH was increased and the dissolution of 2H-MoS was significantly accelerated after SVs formation. Different with pristine form, S-vacant 2H-MoS preferentially harvested proteins (i.e., forming protein corona) involved in antioxidation, photosynthetic electron transport, and the cytoskeleton structure of microalgae. These proteins contain a higher relative number of thiol groups, which exhibited stronger affinity to S-vacant than pristine 2H-MoS, as elucidated by density functional theory calculations. Notably, SVs aggravated algal growth inhibition, oxidative damage, photosynthetic efficiency and cell membrane permeability reduction induced by 2H-MoS due to increased free radical yield and the specific binding of functional proteins. Our findings provide insights into the roles of SVs on the risk of MoS while highlighting the importance of rational design for TMDs application.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126499DOI Listing
October 2021

[Determination of streptomycin and dihydrostreptomycin in grapes by liquid chromatography-tandem mass spectrometry].

Se Pu 2020 Dec;38(12):1396-1401

Institute of Quality and Standard of Agro-Products, Zhejiang Academy of Agricultural Sciences, State Key Laboratory for Managing Biotic and Chemical Theats to the Quality and Safety of Agro-Products, Hangzhou 310021, China.

Streptomycin (STR) and dihydrostreptomycin (DSTR) are two of the most common aminoglycoside antibiotics used in veterinary medicine. STR is produced by some strains, and DSTR is a derivative of STR. In recent years, STR has been widely used in grapes to induce denuclearization. However, high levels of STR may have adverse effects like serious ototoxicity and nephrotoxicity. Therefore, to ensure the quality of grapes and the health of consumers, the regulation of STR and DSTR levels in grapes is required. An analytical method was developed for the identification and quantification of STR and DSTR in grapes by liquid chromatography-tandem mass spectrometry (LC-MS/MS). STR and DSTR are highly polar compounds due to the presence of various amino and hydroxyl groups in their structure. The determination of STR and DSTR poses a considerable analytical challenge, both during sample preparation and instrument analysis. In this study, the main factors governing the response, recovery, and sensitivity of these compounds, such as the type of chromatographic column, the type and proportion of the mobile phase and extraction solvent, the dosage of sodium 1-hexane sulfonate solution, and elution solvent and its volume, were investigated during sample pretreatment and instrument analysis. The STR and DSTR residues in the grape sample were extracted by ultrasonication with a phosphoric acid solution (pH 2), and cleanup and enrichment was performed using an Oasis HLB solid phase column. The analysis was performed using a UPLC Waters HSS T3 column (100 mm×2.1 mm, 1.8 μm) at the column temperature of 35℃. The injection volume was 2 μL. The mobile phase consisted of 0.1% formic acid aqueous solution and methanol with a volume ratio of 60:40. ESI-MS/MS was operated in multiple reaction monitoring (MRM) mode. External standard calibration curves were used for quantification. Based on the optimized method, both analytes displayed good linearity between 2 and 400 μg/L. The correlation coefficients were 0.9991-0.9997. Recoveries in spiked blank grape samples (5, 10, 20, and 40 μg/kg) ranged from 76.8% to 91.9%, with the relative standard deviations (RSDs) less than 10.2%, in compliance with the current legislation. The limits of detection and the limits of quantification of both analytes were 1 μg/L and 5 μg/kg, respectively. To assess the feasibility and potential of the proposed approach for routine analyses of STR and DSTR in other kinds of grape samples, the developed method was applied to the analysis of these compounds in red grapes, xinyu grapes, and xiahei grapes. The recoveries of STR and DSTR in the three kinds of blank grape samples were 77.2%-83.9% and 70.8%-78.9%, respectively, and the RSDs ranged from 3.0% to 15.6%. The results showed that the optimized methods can yield satisfactory recoveries for the analytes in grapes. In this method, the combination of Waters HSS T3 column to overcome the difficulties of the retention and separation of these highly polar compounds in the reverse phase, avoids the use of an ion-pair additive in the mobile phase to increase their retention, which is known to cause severe contamination of the column and serious ion suppression with electrospray ionization detection. In addition, the ideal enrichment and purification effect can be achieved by adding a sodium 1-hexane sulfonate solution to the superstratum extract with the use of only Oasis HLB for sample treatment. The method described herein has the advantages of easy operation, accuracy, and selectivity, making it feasible for the identification and quantification of STR and DSTR residues in grapes.
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http://dx.doi.org/10.3724/SP.J.1123.2020.03034DOI Listing
December 2020

A Lubricating Oil Condition Monitoring System Based on Wear Particle Kinematic Analysis in Microfluid for Intelligent Aeroengine.

Micromachines (Basel) 2021 Jun 25;12(7). Epub 2021 Jun 25.

Civil Aviation Key Laboratory of Aircraft Health Monitoring and Intelligent Maintenance, College of Civil Aviation, Nanjing University of Aeronautics and Astronautics, Nanjing 211100, China.

Lubricating oil monitoring technology is a commonly used method in aeroengine condition monitoring, which includes particle counting technology, as well as spectral and ferrography technology in offline monitoring. However, these technologies only analyze the characteristics of wear particles and rely on physical and chemical analysis techniques to monitor the oil quality. In order to further advance offline monitoring technology, this paper explores the potential role of differences in wear particle kinematic characteristics in recognizing changes in wear particle diameter and oil viscosity. Firstly, a kinematic force analysis of the wear particles in the microfluid was carried out. Accordingly, a microfluidic channel conducive to observing the movement characteristics of particles was designed. Then, the wear particle kinematic analysis system (WKAS) was designed and fabricated. Secondly, a real-time tracking velocity measurement algorithm was developed by using the Gaussian mixture model (GMM) and the blob-tracking algorithm. Lastly, the WKAS was applied to a pin-disc tester, and the experimental results show that there is a corresponding relationship between the velocity of the particles and their diameter and the oil viscosity. Therefore, WKAS provides a new research idea for intelligent aeroengine lubricating oil monitoring technology. Future work is needed to establish a quantitative relationship between wear particle velocity and particle diameter, density, and oil viscosity.
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http://dx.doi.org/10.3390/mi12070748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303112PMC
June 2021

Exploring the Changes of Peroxisomal Polarity in the Liver of Mice with Nonalcoholic Fatty Liver Disease.

Anal Chem 2021 07 30;93(27):9609-9620. Epub 2021 Jun 30.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Biomedical Science, Shandong Normal University, Jinan 250014, People's Republic of China.

Peroxisome proliferator-activated receptor alpha (PPAR-a) is a crucial nuclear transcription regulator of lipid metabolism, which is closely associated with the initiation and development of nonalcoholic fatty liver disease (NAFLD). Because PPAR-a can directly decide the level of peroxisomal metabolic enzymes, its changes might directly cause variations in peroxisomal polarity. Therefore, we developed a new two-photon fluorescence imaging probe, PX-P, in which the triphenylamine and cyanide moieties can real-time sense peroxisomal polarity changes. Using PX-P, we observed a prominent decrease in the peroxisomal polarity in the liver of mice with NAFLD for the first time. More importantly, we discovered that intracellular excessive peroxynitrite (ONOO) and hydrogen peroxide (HO) underwent nitrification and oxidation, respectively, with various sites of PPAR-a. Interestingly, the key site of PPAR-a was nitrated by a low concentration of ONOO rather than being oxidized by the high level of HO. These drastically reduced the activity of PPAR-a, accelerating the occurrence of NAFLD. Moreover, through activating PPARs with pioglitazone, peroxisomal polarity markedly increased compared with that of NAFLD. Altogether, our work presents a new approach for the early diagnosis of NAFLD and identifies potential therapeutic targets.
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http://dx.doi.org/10.1021/acs.analchem.1c01776DOI Listing
July 2021

Establishment of a model for predicting sentinel lymph node metastasis in early breast cancer based on contrast-enhanced ultrasound and clinicopathological features.

Gland Surg 2021 May;10(5):1701-1712

Department of Breast Surgery, Affiliated Cancer Hospital of Zhengzhou University (Henan Cancer Hospital), Zhengzhou, China.

Background: Sentinel lymph node (SLN) biopsy (SLNB) is the standard procedure for axillary staging in clinically node-negative (cN0) breast cancer patients. However, the positive rate of SLNs among cN0 stage patients is 26-35%. The identification of appropriate candidates for SLNB is quite challenging. This study aimed to establish and verify a predictive model of SLN metastasis using contrast-enhanced ultrasound (CEUS) and other clinicopathological indicators.

Methods: The clinicopathological data of 224 patients who had undergone SLNB at the Affiliated Cancer Hospital of Zhengzhou University from June 2018 to July 2019 were analyzed retrospectively. The risk prediction model of SLN metastasis was established by logistic regression analysis. According to the β value of each variable in the model, a risk score system of SLN metastasis was established and verified using the internal population. The predictive model was prospectively applied to 73 patients from July 2019 to September 2019 to evaluate the clinical value of the model in patients with early breast cancer.

Results: Multivariate analysis confirmed that body mass index (BMI), SLN aspect ratio of CEUS mode, SLN aspect ratio of mammography, lympho-vascular invasion, and cytokeratin (CK)5/6 were independent risk factors for SLN metastasis. A scoring system was established according to the above risk factors, and a receiver operating characteristic (ROC) curve was drawn. After internal- and external verification, a corrected ROC curve was drawn, respectively. The ROC curve of the modeling group, internal verification group, and external verification group was 0.9075 (95% CI: 0.8616-0.9534), 0.8766 (95% CI: 0.8192-0.9341), and 0.8505 (95% CI: 0.7333-0.9676), respectively.

Conclusions: We constructed and verified a prediction model of SLN metastasis in early breast cancer. The model has a specific predictive value for preoperative evaluation of SLN status.
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http://dx.doi.org/10.21037/gs-21-245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184381PMC
May 2021

Downregulation of Suppresses the Proliferation and Induces the Apoptosis of Gastric Cancer Cells Through the Hippo Signaling Pathway.

DNA Cell Biol 2021 Aug 22;40(8):1076-1086. Epub 2021 Jun 22.

Department of General Surgery, Peking University Third Hospital, Peking University, Beijing, China.

Fatty acid binding protein 5 () has been reported to play an important role in various cancers. We found that high expression was associated with poor histological differentiation and vascular invasion. High expression indicated a poor prognosis. Downregulation of suppressed cell proliferation, cell migration and invasion, and induced cell apoptosis. Bioinformatic analysis revealed that the Hippo signaling pathway was related to . We found that overexpression of yes-associated protein 1 (YAP1) could partially reverse the effect of knockdown on growth and apoptosis. The inhibitor SBFI-26 suppressed the proliferation and promoted the apoptosis of gastric cancer (GC) cells and interfered with the Hippo signaling pathway by inhibiting YAP1. Our data suggested that might act as a potential target associated with the Hippo signaling pathway for GC treatment.
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http://dx.doi.org/10.1089/dna.2021.0370DOI Listing
August 2021

visualization of peroxisomal viscosity in the liver of mice with non-alcoholic fatty liver disease by near-infrared fluorescence and photoacoustic imaging.

Chem Sci 2020 Oct 6;11(44):12149-12156. Epub 2020 Oct 6.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institutes of Biomedical Sciences, Shandong Normal University Jinan 250014 People's Republic of China

Non-alcoholic fatty liver disease (NAFLD) can gradually develop into hepatic failure, and early diagnosis is crucial to improve treatment efficiency. The occurrence of NAFLD is closely related to lipid metabolism. Peroxisomes act as the first and main site for lipid metabolism in the hepatocytes, so abnormal lipid metabolism might directly affect peroxisomal viscosity. Herein, we developed a new near-infrared fluorescence (NIRF) and photoacoustic (PA) imaging probe (PV-1) for the real-time visualization of peroxisomal viscosity . This PV-1 encompasses the malononitrile group as the rotor, which emits strong NIRF (at 705 nm) and PA (at 680 nm) signals when rotation is hindered as viscosity increases. Through dual-mode imaging, we discovered distinctly higher viscosity in the liver of NAFLD mice for the first time. We further found the remarkable amelioration of NAFLD upon treatment with -acetylcysteine (NAC). Therefore, we anticipate that the PV-1 imaging method is promising for the early diagnosis and prognostic evaluation of NAFLD.
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http://dx.doi.org/10.1039/d0sc02922jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163019PMC
October 2020

Automatic and sensitive detection of West Nile virus non-structural protein 1 with a portable SERS-LFIA detector.

Mikrochim Acta 2021 05 27;188(6):206. Epub 2021 May 27.

Beijing Institute of Radiation Medicine, Beijing, 100850, People's Republic of China.

A portable surface-enhanced Raman scattering (SERS)-lateral flow immunoassay (LFIA) detector has been developed for the automatic and highly sensitive detection of West Nile virus (WNV) non-structural protein 1 (NS1) and actual WNV samples. [email protected] nanoparticles ([email protected] NPs) labeled with double-layer Raman molecules were used as SERS tags to prepare WNV-specific SERS-LFIA strips. On this platform, the WNV-specific antigen NS1 protein was quantitatively and sensitively detected. The detection limit for the WNV NS1 protein was 0.1 ng/mL, which was 100-fold more sensitive than visual signals. The detection limit for inactivated WNV virions was 0.2 × 10 copies/μL. The sensitivity of the SERS-LFIA detector was comparable to that of the fluorescence quantitative reverse transcription-polymerase chain reaction assay. The prepared SERS-LFIA strips exhibited high sensitivity and good specificity for WNV. Thus, the strips developed herein have clinical application value. Moreover, the portable SERS-LFIA detector enabled automatic and rapid detection of the SERS-LFIA strips. The platform established herein is expected to make a substantial contribution to the diagnosis and control of outbreaks of emerging infectious diseases, including WNV.
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http://dx.doi.org/10.1007/s00604-021-04857-3DOI Listing
May 2021

Predicting the 10-year risk of cataract surgery using machine learning techniques on questionnaire data: findings from the 45 and Up Study.

Br J Ophthalmol 2021 May 26. Epub 2021 May 26.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

Background/aims: To investigate the feasibility and accuracy of using machine learning (ML) techniques on self-reported questionnaire data to predict the 10-year risk of cataract surgery, and to identify meaningful predictors of cataract surgery in middle-aged and older Australians.

Methods: Baseline information regarding demographic, socioeconomic, medical history and family history, lifestyle, dietary and self-rated health status were collected as risk factors. Cataract surgery events were confirmed by the Medicare Benefits Schedule Claims dataset. Three ML algorithms (random forests [RF], gradient boosting machine and deep learning) and one traditional regression algorithm (logistic model) were compared on the accuracy of their predictions for the risk of cataract surgery. The performance was assessed using 10-fold cross-validation. The main outcome measures were areas under the receiver operating characteristic curves (AUCs).

Results: In total, 207 573 participants, aged 45 years and above without a history of cataract surgery at baseline, were recruited from the 45 and Up Study. The performance of gradient boosting machine (AUC 0.790, 95% CI 0.785 to 0.795), RF (AUC 0.785, 95% CI 0.780 to 0.790) and deep learning (AUC 0.781, 95% CI 0.775 to 61 0.786) were robust and outperformed the traditional logistic regression method (AUC 0.767, 95% CI 0.762 to 0.773, all p<0.05). Age, self-rated eye vision and health insurance were consistently identified as important predictors in all models.

Conclusions: The study demonstrated that ML modelling was able to reasonably accurately predict the 10-year risk of cataract surgery based on questionnaire data alone and was marginally superior to the conventional logistic model.
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http://dx.doi.org/10.1136/bjophthalmol-2020-318609DOI Listing
May 2021

Axial Length Change in Pseudophakic Eyes Measured by IOLMaster 700.

Transl Vis Sci Technol 2021 05;10(6):29

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China.

Purpose: The purpose of this study was to investigate the change in axial length (AL) after cataract surgery measured by swept source optical coherence tomography (IOLMaster 700), and explore ways to eliminate this AL measurement error in pseudophakic eyes.

Methods: Patients with cataract who underwent unilateral phacoemulsification with four types of intraocular lens (IOLs) implantation (Asphina 509M, Tecnis PCB00, enVista MX60, and Acrysof SN60WF) were enrolled. Bilateral AL measurements were performed before and 1 month after cataract surgery utilizing IOLMaster 700. The postoperative AL of the operated eye was evaluated using three different modes (phakic, aphakic, and pseudophakic), and the fellow eye was measured by phakic mode. Associations among the AL change and cataract grade, lens thickness, preoperative AL, or refractive index of IOL were investigated using stepwise multivariate linear regression.

Results: A total of 305 patients with cataract with mean age of 65.97 ± 13.39 years were recruited. The mean postoperative AL was 0.10 mm and 0.21 mm shorter than the pre-operative AL utilizing pseudophakic and phakic modes, respectively (P < 0.001). No significant difference was observed between pre-operative and postoperative AL using aphakic mode (P = 0.264). There were no significant associations among AL change in pseudophakic eye and cataract grade, lens thickness, pre-operative AL, or refractive index of IOL (P > 0.05).

Conclusions: A correction factor of 0.10 mm is suggested to eliminate AL measurement error of IOLMaster 700 in pseudophakic eyes before further improvement of AL measurement accuracy.

Translational Relevance: Our study may help to eliminate the AL measurement error of IOLMaster 700 in pseudophakic eyes.
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http://dx.doi.org/10.1167/tvst.10.6.29DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161700PMC
May 2021

Effect of High Myopia on Dynamic Changes of Anterior Angle After Pharmacologic Mydriasis in Cataract Patients: A SS-ASOCT Study.

Transl Vis Sci Technol 2021 05;10(6):25

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China.

Purpose: The purpose of this study was to evaluate the effect of high myopia on anterior angle change after pharmacologic mydriasis in patients with cataract using swept-source anterior segment optical coherence tomography (SS-ASOCT).

Methods: This prospective cross-sectional study continuously recruited patients with cataract aged 40 years and older during the period August 2019 to August 2020. The anterior segment parameters, including central corneal thickness (CCT), anterior chamber depth (ACD), angle opening distance (AOD), angle recess area (ARA), trabecular iris space area (TISA), trabecular-iris angle (TIA), angle to angle width (ATA), and anterior chamber volume (ACV), were obtained using SS-ASOCT at baseline and 30 minutes after mydriasis. Regression analyses were performed to identify the factors related to the relative change of AOD500 (ΔAOD500).

Results: A total of 938 patients (938 eyes) were included. The AOD500 decreased from 0.46 ± 0.22 mm to 0.40 ± 0.19 mm, with percent ΔAOD500 of -13.59% ± 37.73% (P = 0.005). The patients with high myopia had a smaller reduction of anterior angle parameters, with a percent ΔAOD500 of -22.74% ± 58.09%% in non-high myopic eyes and -0.84% ± 45.47% in high myopic eyes (P < 0.001). The stepwise multivariate regression demonstrated that the smaller reduction of AOD500 were independently associated with younger age (coefficient = -2.11, 95% confidence interval [CI] = -2.59 to -1.64, P < 0.001), presence of high myopia (coefficient = 15.35, 95% CI = 3.63 to 27.07, P = 0.010), greater baseline TISA500 (coefficient = 60.78, 95% CI = 8.75 to 112.82, P = 0.022), and ATA (coefficient = 11.21, 95% CI = 4.53 to 17.89, P = 0.001).

Conclusions: The anterior chamber angle decreased after pharmacologic mydriasis in these patients with cataract. Angle shallowing after pharmacologic mydriasis was significantly less pronounced in high myopic eyes than in non-high myopic eyes.

Translational Relevance: These findings are informative for the relative less risk of angle-closure glaucoma in highly myopic eyes.
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http://dx.doi.org/10.1167/tvst.10.6.25DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142703PMC
May 2021
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