Publications by authors named "Zhenya Zhang"

182 Publications

Combined effect of zero valent iron and magnetite on semi-dry anaerobic digestion of swine manure.

Bioresour Technol 2021 Nov 28:126438. Epub 2021 Nov 28.

Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province, College of Ecology and Environment, Hainan University, 58 Renmin Avenue, Meilan District, Haikou 570228, PR China. Electronic address:

Combined effect of zero valent iron (ZVI) and magnetite on semi-dry anaerobic digestion of swine manure was studied. Compared with control, the addition of 5 g/L ZVI, magnetite and their mixture (1:1 wt) increased the CH yield by 17.6%, 22.7% and 21.9%, respectively. The three additives improved CH production through altering the metabolism pathways, rather than improving the solid degradation efficiency. ZVI promoted interspecies hydrogen transfer (IHT) by enriching H-comsuming Methanolinea and acetate-oxidizing bacteria (Sedimentibacter and Clostridium). Magnetite enriched dissimilatory iron reduction bacteria (Acinetobacter) to accelerate organic hydrolysis and established direct interspecies electron transfer (DIET) by enriching Methanothrix and Methanospirillum. Key microorganisms relative to IHT (Clostridium) and DIET (Methanothrix and Methanospirillum) were simultaneously enriched with ZVI + magnetite, but they only showed an additive effect on methanogenesis, the lack of synergetic effect was attributable to the possible trade-off between IHT and DIET, or the little improvement effect of additives on substrate biodegradability.
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http://dx.doi.org/10.1016/j.biortech.2021.126438DOI Listing
November 2021

Achieving stably enhanced biological phosphorus removal from aerobic granular sludge system via phosphorus rich liquid extraction during anaerobic period.

Bioresour Technol 2021 Nov 27:126439. Epub 2021 Nov 27.

College of Resources and Environment, University of Chinese Academy of Sciences, Yuquan Road 19A, Beijing 100049, China.

In order to sustainably manage wastewater treatment plants and the environment, enhanced biological phosphorus (P) removal (EBPR) was proposed to achieve P recovery through extracting P-rich liquid (i.e., Phostrip) from the bottom of aerobic granular sludge (AGS)-based sequencing batch reactors (SBRs) under no mixing during the anaerobic phase. Results showed both tested bacterial AGS (BAGS) and algal-bacterial AGS (A-BAGS) systems stably produced low effluent P (< 0.05 mg-P/L) with little impact on their organics and NH-N removals (> 99%). The collected P-rich liquids (55-83 mg-P/L) from both systems showed great potential for P recovery of about 83.85 ± 0.57 % (BAGS) or 83.99 ± 0.77% (A-BAGS), which were contributed by the influent P (> 95%) and P reserves in granules based on P balance analysis. This study suggests that the AGS-based SBRs coupling the Phostrip holds great potentials for P recovery profit and further reduction in energy consumption.
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http://dx.doi.org/10.1016/j.biortech.2021.126439DOI Listing
November 2021

Simultaneous recovery of phosphorus and alginate-like exopolysaccharides from two types of aerobic granular sludge.

Bioresour Technol 2021 Nov 25:126411. Epub 2021 Nov 25.

Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan; Department of Mechanical Engineering, City University of Hong Kong, Kowloon Tang, Hong Kong.

Wastewater treatment plants are expected to realize not only pollutants removal from wastewater but also resources recovery such as phosphorus (P) and alginate-like exopolysaccharides (ALE) from the produced sludge. In this study, ALE extraction and fractionation from the same activated sludge-derived bacterial aerobic granular sludge (AGS) and algal-bacterial AGS were performed in addition to P fate examination during ALE recovery. Results showed that the ALE content recovered from algal-bacterial AGS was 8.81 ± 0.02 mg/g-volatile suspended solids (VSS), about 2.8 times higher than that from bacterial AGS when fed with the same synthetic wastewater. Moreover, the mannuronic acid to guluronic acid (MG) blocks accounted for the largest proportion of ALE from the two granular sludges. In particular, the extracellular polymeric substances (EPS) extracted from bacterial and algal-bacterial AGS contained about 25.10 ± 1.85 and 19.53 ± 0.04 mg-P/g-SS, respectively, and both granular sludges possessed high P bioavailability of 97-99%.
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http://dx.doi.org/10.1016/j.biortech.2021.126411DOI Listing
November 2021

A review on biogas upgrading in anaerobic digestion systems treating organic solids and wastewaters via biogas recirculation.

Bioresour Technol 2021 Nov 24:126412. Epub 2021 Nov 24.

Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan; Department of Mechanical Engineering, City University of Hong Kong, Kowloon Tang, Hong Kong.

Biogas upgrading is an essential process for efficient and safe utilization of biogas produced from anaerobic digestion (AD), a cost-effective and environmentally friendly technology for bioenergy recovery from organic wastes. Biogas recirculation in AD reactors has been recently reported as a cost-effective and promising method to enhance methane content in biogas. This review aimed to summarize the state-of-the-art of biogas recirculation-based AD systems to better understand the possible mechanisms and main factors relating to in-situ biogas upgrading. It shows that biogas recirculation in the AD reactor can not only enhance methane content via both physicochemical and biological effects, but also help establish a robust AD system with high buffering capacity for highly efficient treatment of various organic wastes. More research works are demanding for a better understanding of the mechanisms and the optimization of the whole AD system, targeting its further development for high-calorie bioenergy production.
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http://dx.doi.org/10.1016/j.biortech.2021.126412DOI Listing
November 2021

Current status of total neoadjuvant therapy for locally advanced rectal cancer.

Asia Pac J Clin Oncol 2021 Nov 24. Epub 2021 Nov 24.

Department of Radiation Oncology, Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital & Hebei Clinical Research Center for Radiation Oncology, Shijiazhuang, China.

Neoadjuvant chemoradiotherapy (nCRT) plus total mesorectal excision (TME) has been the standard regimen for treatment of patients with locally advanced rectal cancer (LARC), because it significantly reduces the rate of local recurrence and enables sphincter preservation. However, distant metastasis remains the major reason for treatment failure, and the value of postoperative chemotherapy is still controversial. Recent studies have examined the use of total neoadjuvant therapy (TNT), defined as induction and/or consolidation chemotherapy (CONCT) with radiotherapy (RT) or nCRT prior to surgery. The results indicated that TNT may increase the rates of chemotherapy compliance and pathological complete response (pCR), and probably improve the success rate of sphincter preservation surgery. TNT may also improve disease-free survival and overall survival, and even reduce the rate of relapse. Here, we critically appraise the existing literature on three different TNT schemes used for LARC patients.
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http://dx.doi.org/10.1111/ajco.13640DOI Listing
November 2021

Supplementation of KOH to improve salt tolerance of methanogenesis in the two-stage anaerobic digestion of food waste using pre-acclimated anaerobically digested sludge by air-nanobubble water.

Bioresour Technol 2021 Nov 18:126360. Epub 2021 Nov 18.

Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan. Electronic address:

Air-nanobubble water (NBW) was applied to pre-acclimate anaerobically digested sludge that was then used as the inoculum in the two-stage anaerobic digestion (AD) of high saline (20 g NaCl/L) food waste (FW) to optimize NBW application in the AD of high saline FW. K was simultaneously supplemented during the methanogenic stage to resist the inhibition of salt on methanogens. Results showed that after the second pre-acclimation cycle, the inoculum activity was increased 27% in the Air-NBW supplemented reactor in comparison to the deionized water (DW) supplemented one. In the first-stage AD, H yield was enhanced by 46% in the Air-NBW pre-acclimated sludge reactor compared with the DW pre-acclimated sludge reactor. Besides, supplementation of KOH in the methanogenic stage could enhance methane production by 17-25% in the DW reactors at initial pH 7.5, 8.0, and 9.0 when compared to the control reactor (using NaOH adjusted initial pH to 7.5), respectively.
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http://dx.doi.org/10.1016/j.biortech.2021.126360DOI Listing
November 2021

Dynamics of the prokaryotic and eukaryotic microbial community during a cyanobacterial bloom.

Biosci Biotechnol Biochem 2021 Oct 18. Epub 2021 Oct 18.

Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai Tsukuba, Ibaraki, Japan.

Toxic cyanobacterial blooms frequently develop in eutrophic freshwater bodies worldwide. Microcystis species produce microcystins (MCs) as a cyanotoxin. Certain bacteria that harbor the mlr gene cluster, especially mlrA, are capable of degrading MCs. However, MCs-degrading bacteria may possess or lack mlr genes (mlr+ and mlr- genotypes, respectively). In this study we investigated the genotype that predominantly contributes to biodegradation and cyanobacterial predator community structure with change in total MCs concentration in an aquatic environment. The two genotypes co-existed but mlr+ predominated, as indicated by the negative correlation between mlrA gene copy abundance and total MCs concentration. At the highest MCs concentrations, predation pressure by Phyllopoda, Copepoda, and Monogononta (rotifers) was reduced; thus, MCs may be toxic to cyanobacterial predators. The results suggest cooperation between MCs-degrading bacteria and predators may reduce Microcystis abundance and MCs concentration.
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http://dx.doi.org/10.1093/bbb/zbab179DOI Listing
October 2021

Therapy Strategy of CD47 in Diffuse Large B-Cell Lymphoma (DLBCL).

Dis Markers 2021 16;2021:4894022. Epub 2021 Sep 16.

Department of Hematology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050000 Hebei Province, China.

At present, the use of the common chemotherapy regimen CHOP/R-CHOP for diffuse large B-cell lymphoma (DLBCL) has some shortcomings, especially for relapsed and refractory DLBCL. CD47 is now considered as a prominent target in cancer therapies, and CD47 blockade mainly inhibits the CD47-SIRP axis to prevent tumor immune escape. Here, we evaluated the effects of the latest CD47 antibodies reported and the correlations of closely related genes with CD47 in this disease. In the future, therapeutic strategies for DLBCL will focus on multitarget antibody combined treatment and multigene joint attacks.
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http://dx.doi.org/10.1155/2021/4894022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463246PMC
September 2021

Temperature affects growth, geosmin/2-methylisoborneol production, and gene expression in two cyanobacterial species.

Environ Sci Pollut Res Int 2021 Sep 23. Epub 2021 Sep 23.

Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, Japan.

Cyanobacterial blooms accompanied by taste and odor (T&O) compounds affect the recreational function and safe use of drinking water. Geosmin and 2-methylisoborneol (2-MIB) are the most common T&O compounds. In this study, we investigated the effect of temperature on geosmin and 2-MIB production in Dolichospermum smithii and Pseudanabaena foetida var. intermedia. More specifically, transcription of one geosmin synthase gene (geoA) and two 2-MIB synthase genes (mtf and mtc) was explored. Of the three temperatures (15, 25, and 35 °C) tested, the maximum Chl-a content was determined at 25 °C in both D. smithii and P. foetida var. intermedia. The maximum total geosmin concentration (19.82 μg/L) produced by D. smithii was detected at 25 °C. The total 2-MIB concentration (82.5 μg/L) produced by P. foetida var. intermedia was the highest at 35 °C. Besides, the lowest Chl-a content and minimum geosmin/2-MIB concentration were observed at 15 °C. There was a good positive correlation between geosmin/2-MIB concentration and Chl-a content. The expression levels of the geoA, mtf, and mtc genes at 15 °C were significantly higher than those at 25 and 35 °C. The transcription of the mtf and mtc genes in P. foetida var. intermedia was higher at 35 °C than at 25 °C. The results highlight unfavorable temperature can increase the potential of geosmin/2-MIB synthesis from the gene expression level in cyanobacteria. This study could provide basic knowledge of geosmin/2-MIB production by cyanobacteria for better understanding and management of T&O problems in drinking water.
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http://dx.doi.org/10.1007/s11356-021-16593-5DOI Listing
September 2021

Cr(VI) bioremediation by active algal-bacterial aerobic granular sludge: Importance of microbial viability, contribution of microalgae and fractionation of loaded Cr.

J Hazard Mater 2021 09 6;418:126342. Epub 2021 Jun 6.

Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.

In this study, chromium (Cr) was used as an example of the most toxic heavy metals that threaten human health, and Cr(VI) bioremediation was implemented by using a new type of aerobic granular sludge (AGS), i.e., algal-bacterial AGS. Results showed that the total Cr removal efficiency by active algal-bacterial AGS was 85.1 ± 0.6% after 6 h biosorption at pH 6 and room temperature, which could be further improved to 93.8 ± 0.4% with external electron donor (glucose) supply. However, inactivation dramatically decreased the total Cr removal efficiency to 29.6 ± 3.5%, and no effect was noticed when external electron donor was provided. With an antibiotic (levofloxacin) or metabolic inhibitor (NaN) addition, the total Cr removal efficiency of bacterial AGS was inhibited by 16.0% or 10.1%, but this efficiency was maintained in the case of algal-bacterial AGS. Analysis of extracellular polymeric substances (EPS) composition revealed that under Cr(VI) exposure, more loosely bound EPS were secreted by algal-bacterial AGS, favoring Cr(VI) reduction. Results from chemical fractionation indicated that 90.5 ± 4.2% of the loaded Cr on algal-bacterial AGS was in an immobile form, reflecting the low environmental risk of Cr-loaded algal-bacterial AGS after biosorption of hazardous heavy metals from wastewater.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126342DOI Listing
September 2021

Plasma Lipidomics Profiling Reveals Biomarkers for Papillary Thyroid Cancer Diagnosis.

Front Cell Dev Biol 2021 21;9:682269. Epub 2021 Jun 21.

Zhongguancun Biological and Medical Big Data Center, Beijing, China.

The objective of this study was to identify potential biomarkers and possible metabolic pathways of malignant and benign thyroid nodules through lipidomics study. A total of 47 papillary thyroid carcinomas (PTC) and 33 control check (CK) were enrolled. Plasma samples were collected for UPLC-Q-TOF MS system detection, and then OPLS-DA model was used to identify differential metabolites. Based on classical statistical methods and machine learning, potential biomarkers were characterized and related metabolic pathways were identified. According to the metabolic spectrum, 13 metabolites were identified between PTC group and CK group, and a total of five metabolites were obtained after further screening. Its metabolic pathways were involved in glycerophospholipid metabolism, linoleic acid metabolism, alpha-linolenic acid metabolism, glycosylphosphatidylinositol (GPI)-anchor biosynthesis, Phosphatidylinositol signaling system and the metabolism of arachidonic acid metabolism. The metabolomics method based on PROTON nuclear magnetic resonance (NMR) had great potential for distinguishing normal subjects from PTC. GlcCer(d14:1/24:1), PE-NME (18:1/18:1), SM(d16:1/24:1), SM(d18:1/15:0), and SM(d18:1/16:1) can be used as potential serum markers for the diagnosis of PTC.
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http://dx.doi.org/10.3389/fcell.2021.682269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255691PMC
June 2021

Selective Adsorption of Potassium in Seawater by CoHCF Thin Film Electrode and Its Electrochemical Desorption/Regeneration.

Materials (Basel) 2021 Jun 27;14(13). Epub 2021 Jun 27.

Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba 305-8565, Japan.

Cobalt Hexacyanoferrate (CoHCF) was tested for the selective uptake of K from seawater and the electrochemical method was adopted for the desorption and regeneration of the material. Powder form CoHCF could adsorb about 6.5 mmol/g of K from the seawater. For the ease of the electrochemical desorption and regeneration, CoHCF thin film was coated onto the Indium Tin Oxide (ITO) glass to obtain a CoHCF electrode. K adsorption kinetics on CoHCF thin film was found to be well fitted with the intraparticle diffusion model, which was a two-step process. Five consecutive adsorption-desorption-regeneration cycles were carried out to know the gradual decrease in the adsorption capacity owing to changes in the redox states of two metals, Co and Fe, in the material. Fourier Transform Infrared Spectroscopy (FT-IR) and Ultraviolet-Visible (UV-Vis) measurement results corresponded to the color change of CoHCF thin film, indicating the valence change of transition metals and the exchange of alkali metal cations happened on the CoHCF at different operation stages. In order to elucidate the reaction mechanism, composition of the material was analysis in the following steps: adsorption, desorption, and regeneration. It was proved that the system based on CoHCF thin film modified electrode had the potential of recovering potassium from seawater.
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http://dx.doi.org/10.3390/ma14133592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269628PMC
June 2021

Comparison of decabromodiphenyl ether degradation in long-term operated anaerobic bioreactors under thermophilic and mesophilic conditions and the pathways involved.

J Environ Manage 2021 Sep 11;294:113009. Epub 2021 Jun 11.

Material Cycles Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan; Fujian Ospring Technology Development Co., Ltd., No. 22 Jinrong North Road Cangshan District, Fuzhou, 350000, PR China. Electronic address:

Anaerobic digestion of decabromodiphenyl ether was carried out and compared in two continuously stirred anaerobic bioreactors for 210 days under thermophilic and mesophilic conditions. Results show that the degradation of decabromodiphenyl ether followed the first-order reaction kinetics, which exhibited a higher removal rate in the thermophilic reactor when compared to the mesophilic one, reaching its maximum of 1.1 μg·day. The anaerobic digestion of decabromodiphenyl ether was found to involve the replacement of bromines from polybrominated diphenyl ether by hydrogen atoms, gradually forming nona-, octa- and hepta-brominated diphenyl ether, respectively. Under the thermophilic condition, the reactors were dominated by Bacillus sp. and Methanosarcina sp. with high bioactivity and high concentrations of debromination microorganisms.
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http://dx.doi.org/10.1016/j.jenvman.2021.113009DOI Listing
September 2021

Low Dose of Fluoride in the Culture Medium of Promotes Its Growth and Enhances Bioactives with Antioxidant and Anticancer Properties.

J Fungi (Basel) 2021 Apr 28;7(5). Epub 2021 Apr 28.

Graduate School of Life & Environmental Science, University of Tsukuba, Ibaraki 305-8575, Japan.

possesses several compounds with medicinal properties, and is commonly used in traditional Chinese functional food and medicine for a variety of health benefits. Because of its rare occurrence in nature, the market demand for artificial is on the rise. Furthermore, efforts to increase its bioactive ingredients have also been considered in research. In this study, we aimed to investigate the effect of fluoride on the growth and enrichment of bioactive compounds in . A wide range of potassium fluoride concentrations (0, 0.001, 0.01, 0.1, and 1 mM) were added to the culture media as a source of fluoride during the cultivation of fruiting bodies. The contents of fluorine and bioactive substances of the fruiting bodies in normal (NM) and fluorine-supplemented (FM) media were measured and compared. raised in growth medium supplemented with 0.01 mM potassium fluoride led to a 44.86% (1.55 ± 0.14 g/bottle) increase in biomass and a 23.43% (3161.38 ± 35.71 µg/g) increase in total carotenoid content in the fruiting bodies. Furthermore, a remarkable increase in superoxide dismutase-like activity (84.75 U/mg) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (IC = 2.59 mg/mL) was recorded. In human cancer cell-based assays, raised in FM caused stronger cytotoxicity, apoptosis, and cell cycle arrest in human osteosarcoma cells. These results demonstrated that a low dose of fluoride could stimulate the growth of fruiting bodies and enhance the production of bioactive ingredients that possess useful antioxidant and anticancer activities.
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http://dx.doi.org/10.3390/jof7050342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145312PMC
April 2021

Alleviation of ammonia inhibition via nano-bubble water supplementation during anaerobic digestion of ammonia-rich swine manure: Buffering capacity promotion and methane production enhancement.

Bioresour Technol 2021 Aug 7;333:125131. Epub 2021 Apr 7.

Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan. Electronic address:

Anaerobic digestion (AD) of ammonia-rich swine manure (SM) with nano-bubble water (NBW) supplementation was studied in this work with the expectation of ammonia inhibition alleviation, buffering capacity promotion, and methane production enhancement. Results indicated that cumulative methane yield was elevated by 12.3-38.7% in NBW groups. Besides, the reduced methane production rate and elongated lag phase under ammonia inhibition were increased and shortened by NBW supplementation, respectively. The rapid increase of total alkalinity (TA) and partial alkalinity (PA) could be observed with NBW supplementation, as well as the rapid decline of VFA/TA, thus improved buffering capacity and alleviated ammonia inhibition. Moreover, higher level of extracellular hydrolases and coenzyme F could be detected in NBW groups. In conclusion, NBW with higher mobility and zeta potential (absolute value) could be a promising strategy for the alleviation of ammonia suppression during the AD of SM.
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http://dx.doi.org/10.1016/j.biortech.2021.125131DOI Listing
August 2021

Performance and stability of biogas recirculation-driven anaerobic digestion system coupling with alkali addition strategy for sewage sludge treatment.

Sci Total Environ 2021 Aug 7;783:146966. Epub 2021 Apr 7.

Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan. Electronic address:

Wastewater treatment plants are particularly challenging with the treatment and disposal of sewage sludge produced from the treatment units due to its high costs and environmental hazards. In this study, a biogas recirculation-driven anaerobic digestion (AD) system was developed with upward shear force being provided by biogas recirculation coupled with the alkali addition strategy, targeting biogas upgrading, sludge stabilization, and sludge flocculation simultaneously, thus reducing the sludge management costs. Compared to the conventional AD system, the novel biogas recirculation-driven AD system could achieve biogas upgrading with 10% higher CH content. Besides, the combination of NaOH and Ca(OH) addition strategy obviously improved sludge settleability and dewaterability compared to the single NaOH addition strategy. Owing to the attraction between negatively charged sludge particles and Ca ions, the available Ca in the former AD system may facilitate the re-flocculation and P immobilization in solid digestate, fix partial CO with less CO emission, and bridge with some sludge flocs. Moreover, 12.6% lower net cost for sludge management was achieved by this biogas recirculation-driven AD system together with the combination alkali addition strategy, which is regarded as a promising integrated multi-purpose system for sludge treatment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146966DOI Listing
August 2021

CD151 promotes Colorectal Cancer progression by a crosstalk involving CEACAM6, LGR5 and Wnt signaling via TGFβ1.

Int J Biol Sci 2021 17;17(3):848-860. Epub 2021 Feb 17.

Department of Pharmacology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, 050200, China.

CD151 impacts various signaling pathways in different cancers, and promotes colorectal cancer (CRC) cell malignancy by yet undefined mechanisms. This study aimed to comprehensively assess CD151's function in CRC. CD151 levels were significantly higher in CRC tissues and cells compared with controls in the tissue microarray. Cell viability, migration and invasion were suppressed by CD151 downregulation in CRC cells. Consistently, mouse xenografts were inhibited by CD151 silencing. RNA-seq revealed that multiple genes were significantly altered by CD151 knockdown in cultured CRC cells and xenografts. Particularly, transforming growth factor β1 (TGFβ1), carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) and leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) alongside CD151 were downregulated both and . Co-immunoprecipitation and mass spectrometry results were validated by qRT-PCR and immunoblot. Moreover, pull-down assay and immunofluorescence confirmed the associations of TGFβ1, CEACAM6 and LGR5 with CD151. This study demonstrated CEACAM6, LGR5 and Wnt pathway suppression by CD151 silencing might occur through TGFβ1 regulation, offering a comprehensive view of CD151's roles in colorectal carcinogenesis. Our findings provide an insight into the CD151-involved signaling network in CRC oncogenesis, which could be utilized to design novel targeted therapies against CD151-based signaling in treatment for CRC.
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http://dx.doi.org/10.7150/ijbs.53657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7975690PMC
February 2021

Insight into Cr(VI) biosorption onto algal-bacterial granular sludge: Cr(VI) bioreduction and its intracellular accumulation in addition to the effects of environmental factors.

J Hazard Mater 2021 07 22;414:125479. Epub 2021 Feb 22.

Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.

Hexavalent chromium (Cr(VI)) is one of the typical heavy metals that pose a great threat to the environment. As a novel biotechnology, algal-bacterial aerobic granular sludge (AGS) possesses the merits of both bacterial AGS and algae. This study firstly evaluated Cr(VI) removal via biosorption by algal-bacterial AGS under different operation conditions and then some environmental factors. Results show that the highest Cr(VI) reduction (99.3%) and total Cr removal (89.1%) were achieved within 6 h at pH 2 and 6, respectively. The coexisting oxyanions exhibited slight effects, while both tested natural organic matters (humic acid and tannic acid) and carbon sources promoted Cr(VI) reduction at some appropriate concentrations. The coexistence of metal cations favored Cr(VI) reduction, achieving the highest enhancement of 8.1% by Cu at 5 mg/L, while the total Cr removal was suppressed to some extent. Salinity > 5 g/L severely inhibited both Cr(VI) reduction and total Cr removal. Moreover, the loaded Cr in algal-bacterial AGS was found to be almost in the form of Cr(III), with 66.8% being contributed by intracellular accumulation. This work suggests that Cr(VI) reduction and intracellular accumulation are the main mechanisms involved in Cr(IV) biosorption onto algal-bacterial AGS.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125479DOI Listing
July 2021

Novel insight into enhanced recoverability of acidic inhibition to anaerobic digestion with nano-bubble water supplementation.

Bioresour Technol 2021 Apr 29;326:124782. Epub 2021 Jan 29.

Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan. Electronic address:

Nano-bubble water (NBW) has been proven to be effective in promoting organics utilization and CH production during anaerobic digestion (AD) process, suggesting its potential in improving the stability of the AD process and thereby alleviating acidic inhibition. In this work, the effect of NBW on digestion stability and CH production was investigated to evaluate the ability of NBW on AD recovery from acidic inhibition. Results showed that NBW supplementation increased the total alkalinity (TA) and partial alkalinity (PA), and reduced the ratio of VFA/TA, thus maintained the stability of the AD process. Generation/consumption of VFAs was also enhanced with NBW supplementation under acidic inhibition with pH values of 5.5, 6.0 and 6.5. The cumulative CH production was 246-257 mL/g-VS in NBW groups, which was 12.1-17.2% higher than the control. Moreover, with NBW supplementation, the maximum CH production rate was raised according to the modeling results.
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http://dx.doi.org/10.1016/j.biortech.2021.124782DOI Listing
April 2021

Response and recovery of mature algal-bacterial aerobic granular sludge to sudden salinity disturbance in influent wastewater: Granule characteristics and nutrients removal/accumulation.

Bioresour Technol 2021 Feb 3;321:124492. Epub 2020 Dec 3.

Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.

The impact of sudden salinity (1-3%) disturbance in influent wastewater on mature algal-bacterial aerobic granular sludge (AGS) was investigated, in addition to its recovery possibility when salinity disturbance was removed. Results show that the mature algal-bacterial AGS with less filamentous could maintain its good settleability with sludge volume index below 41 mL/g when wastewater salinity was increased to 3%, in which loosely bound extracellular polymeric substances might play an important role. Under this condition, the granule system achieved slightly lower dissolved organic carbon removal (from 97% to 94%), while the removals of ammonia nitrogen, total nitrogen and total phosphorus were remarkably decreased from ~100%, 66% and 70% to 23%, 16% and 38%, respectively. However, the organics and nutrients removals could be recovered immediately when the salinity disturbance was removed from the influent. P bioavailability, on the other hand, kept almost stable (93-97%) in the AGS during the examination period.
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http://dx.doi.org/10.1016/j.biortech.2020.124492DOI Listing
February 2021

Human health risk of vanadium in farmland soils near various vanadium ore mining areas and bioremediation assessment.

Chemosphere 2021 Jan 4;263:128246. Epub 2020 Sep 4.

Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.

Various kinds of vanadium (V) ore mining areas produced serious contamination have been widely recognized, while less relevant research was about the associated health risk and V distribution level for farmland soils around. This study assessed the contamination characteristics and associated human health risk of V in the surface farmland soils near various V ore mining areas. The bioremediation of V contamination by indigenous microbes from them was also evaluated. The farmland soils near stone coal area (Hunan province, China) showed the highest mean concentration of V (543.91 mg/kg), posing high non-carcinogenic risks, with high hazard quotient (HQ) value of 1.29 for children. While, V values of sampled soils near V titanomagnetite, petroleum associated minerals and uvanite areas were lower than that near stone coal area, also with lower HQ values (<1.00). Within 60 h, the removal efficiency of V(V) reached 98.4% with farmland soils near uvanite area, suggesting feasibility of V bioremediation via indigenous microbes. Bacterial communities after long-term cultivation (240 d) with V(V) were dominated by native microbes able to tolerate or reduce the toxicity of V(V), such as Ruminococcaceae_incertae_sedis, Trichococcus and Comamonas. This work is helpful for calling attention to V pollution of farmland near various V ore mining areas and formulating effective strategies for V(V) contamination bioremediation.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128246DOI Listing
January 2021

Enhanced energy recovery via separate hydrogen and methane production from two-stage anaerobic digestion of food waste with nanobubble water supplementation.

Sci Total Environ 2021 Mar 26;761:143234. Epub 2020 Oct 26.

Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.

This study investigated the enhancement effect of N- and Air-nanobubble water (NBW) supplementation on two-stage anaerobic digestion (AD) of food waste (FW) for separate production of hydrogen and methane. In the first stage for hydrogen production, the highest cumulative H yield (27.31 ± 1.21 mL/g-VS) was obtained from FW + Air-NBW, increasing by 38% compared to the control (FW + deionized water (DW)). In the second stage for methane production, the cumulative CH yield followed a descending order of FW + Air-NBW (373.63 ± 3.58 mL/g-VS) > FW + N-NBW (347.63 ± 7.05 mL/g-VS) > FW + DW (300.93 ± 3.24 mL/g-VS, control), increasing by 24% in FW + Air-NBW and 16% in FW + N-NBW compared to the control, respectively. Further investigations indicate that different gas-NBW may positively impact the different stages of AD process. Addition of N-NBW only enhanced the hydrolysis/acidification of FW with no significant effect on methanogenesis. By comparison, addition of Air-NBW promoted both hydrolysis/acidification stage and methanogenesis stage, reflecting by the enhanced activities of four extracellular hydrolases at the end of hydrolysis/acidification and coenzyme F at the end of methanogenesis, respectively. Results from this work suggest the potential application of Air-NBW in the two-stage AD for efficient renewable energy recovery from FW.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143234DOI Listing
March 2021

Ionic response of algal-bacterial granular sludge system during biological phosphorus removal from wastewater.

Chemosphere 2021 Feb 5;264(Pt 2):128534. Epub 2020 Oct 5.

Department of Chemical Engineering, National Taiwan University, Taipei, 10617, Taiwan; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, 10607, Taiwan.

Biological phosphorus removal (BPR) from wastewater can be generally realized through alternative non-aeration and aeration operation to create anaerobic and aerobic conditions respectively for P release and uptake/accumulation by polyphosphate accumulating organisms (PAOs), with P removal finally achieved by controlled discharge of P-rich sludge. In this study, the response of algal-bacterial aerobic granular sludge (AB-AGS) during BPR to main ions including Ac (acetate), Cl, SO, NH, K, Mg, Ca and Na in wastewater was investigated with conventional bacterial AGS (B-AGS) as control and acetate as the sole carbon source. Results show that BPR process mainly involved the changes of Ac, K, Mg, and Ca rather than Cl, SO, NH and Na. The mole ratio of ΔP/ΔAc kept almost unchanged during the non-aeration (P release) phase in both B-AGS and AB-AGS systems (ΔP/ΔAc > ΔP/ΔAc), and it was negatively influenced by the light in AB-AGS systems, in which 62% of acetate was not utilized for P release at the high illuminance of 81 k lux. During the entire non-aeration/aeration period, both ΔK/ΔP and ΔMg/ΔP remained constant, while ΔK/ΔP > ΔK/ΔP and ΔMg/ΔP ≈ ΔMg/ΔP. The presence of algae seemed not beneficial for PAOs to remove P, while more K and P uptake by algae in AB-AGS suggest its great potential for manufacturing biofertilizer.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128534DOI Listing
February 2021

Behavior of algal-bacterial granular sludge in a novel closed photo-sequencing batch reactor under no external O supply.

Bioresour Technol 2020 Dec 29;318:124190. Epub 2020 Sep 29.

Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.

Algal-bacterial aerobic granular sludge (AB-AGS) as a symbiosis system possesses high potential for being operated without external O supply. In this study, a novel lab-scale closed photo-sequencing batch reactor (PSBR) was developed for AB-AGS operation under successively open (Phase Ⅰ) and closed (Phase Ⅱ) conditions. Results show that AB-AGS maintained almost 100% of organics removal, exhibiting higher removals of phosphate (63 ± 20%), K (19 ± 12%) and Mg (26 ± 12%), and higher chlorophylls content during Phase II. Meanwhile, only O besides N was detectable in the headspace of PSBR. The change of granular structure and faster algae growth during Phase Ⅱ may contribute to the increase of microbial activity and phosphorus bioavailability, in which lower extracellular polymeric substances content may account for low biomass retention. Results from this closed PSBR imply that AB-AGS has the potential to reduce some greenhouse gases like CO and CH emission.
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http://dx.doi.org/10.1016/j.biortech.2020.124190DOI Listing
December 2020

Investigation of Inhibition Effect of Gossypol-Acetic Acid on Gastric Cancer Cells Based on a Network Pharmacology Approach and Experimental Validation.

Drug Des Devel Ther 2020 7;14:3615-3623. Epub 2020 Sep 7.

The Third Hospital of Hebei Medical University, Shijiazhuang 050011, People's Republic of China.

Background: Gastric cancer (GC) is one of the major public health problems worldwide with high morbidity and mortality. Nowadays, traditional medicine may hold promise for the treatment of cancers. Gossypol-acetic acid (GAA) is a male contraceptive agent that shows anti-tumor effects on multiple types of cancers. However, whether GAA would inhibit the progression of GC remained unclear.

Methods: The potential targets of GAA were predicted by the Pharmmapper software and GC-related genes were obtained from the GeneCard database. The "GC-targets-GAA" network was constructed using the Cytoscape software. The PPI analysis of intersection genes was performed using the String software. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the DAVID software to explore the potential mechanism underlying the regulatory role of GAA in GC. The MTS test, plate cloning test, cell cycle and apoptosis assays were used to verify the function of GAA in GC.

Results: Ten hub genes related to cell cycle progression and apoptosis were identified. Many cancer-related signaling pathways were visualized by the Cytoscape software. Among them, the PI3K-Akt signaling pathway was the highest-ranked pathway. The MTS test and plate cloning test showed that GAA inhibited the proliferation of GC cells. The cell cycle and apoptosis assays showed that GAA induced G1 phase cell cycle arrest and apoptosis in GC cells.

Conclusion: Our study demonstrated the anti-tumor effect of GAA on GC through multiple targets and signaling pathways. These results provided a theoretical basis for further investigation of GAA in preclinical and clinical studies, and suggested the potential use of GAA as a novel therapeutic agent for the treatment of GC.
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http://dx.doi.org/10.2147/DDDT.S256566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489940PMC
July 2021

Insight into efficient phosphorus removal/recovery from enhanced methane production of waste activated sludge with chitosan-Fe supplementation.

Water Res 2020 Dec 19;187:116427. Epub 2020 Sep 19.

Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan. Electronic address:

Fe(III)-loaded chitosan (CTS-Fe) composite was used for the first time to remove and recover phosphorus (P) from waste activated sludge (WAS) via anaerobic digestion (AD). The P transformation pathway and the effect of CTS-Fe addition on the AD process were investigated using batch experiments. The P fractionation results indicate that non-apatite inorganic phosphorus (NAIP) reduction in the solid phase of sludge at 20 g/L of CTS-Fe addition (6.72 mg/g-SS) was 2.4 times higher than that in the control (2.77 mg/g-SS, no CTS-Fe addition). This is probably brought about by the added CTS-Fe enhanced the reduction of Fe(III)-P compounds in the sludge with phosphate released into the liquid phase. CTS-Fe can efficiently recover 95% of P from the liquid digestate of WAS. Notably, partial Fe(III) on the CTS-Fe was reduced and effectively combined with P to form vivianite crystals on the CTS-Fe surface during the AD process. Characterization analysis demonstrated that ligand exchange and chemical precipitation were the dominant mechanisms for P removal/recovery. Furthermore, the addition of CTS-Fe increased methane production by 11.9 - 32.2% under the tested conditions, likely attributable to the enhanced hydrolysis of WAS under CTS-Fe supplementation. As the P-loaded CTS-Fe particles can be easily separated and recovered from the AD system and further reutilized in agriculture, this study could provide a new approach for simultaneous P removal/recovery and enhanced methane production from AD of WAS.
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http://dx.doi.org/10.1016/j.watres.2020.116427DOI Listing
December 2020

Demethylzeylasteral inhibits cell proliferation and enhances cell chemosensitivity to 5-fluorouracil in Colorectal Cancer cells.

J Cancer 2020 19;11(20):6059-6069. Epub 2020 Aug 19.

The Third Hospital of Hebei Medical University, Shijiazhuang, 050011, China.

Malignant growth and chemotherapy resistance to 5-fluorouracil (5-FU) are the obstacles to the treatment of Colorectal cancer (CRC). There is need to develop effective therapeutic option. Demethylzeylasteral benefits to immune and anti-tumor function. However, the role demethylzeylasteral played in colorectal cancer remains unclear. Here, our study confirmed that demethylzeylasteral could inhibit the cell malignant capacity, such as proliferation, migration and invasion. And we also found demethylzeylasteral could cause cell cycle arrest and apoptosis. Followed we verified that combination demethylzeylasteral with 5-FU has a better curative effect . The two drugs function synergistically in SW480 and additionally in RKO. IC50 values of 5-FU decreased when combined with demethylzeylasteral. Next, we used the network pharmacology approach to explore the the potential molecular mechanism of demethylzeylasteral. We constructed the "Colorectal - targets - Demethylzeylasteral" and protein-protein interactions (PPI) networks. And 15 hub genes were found in PPI network. Then Gene Ontology (GO) enrichment analysis showed that demethylzeylasteral may affect cell cycle, apoptosis, invasion and response to chemotherapy drugs. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated demethylzeylasteral may be involved in many cancer-related pathways. Taken together, the network pharmacology approach provided a potential mechanism of demethylzeylasteral in colorectal cells. Our study indicated that demethylzeylasteral could exert anti-tumor effects and enhance the sensitivity of the Colorectal cells to 5-FU, suggesting a promising ability to serve as an anti-cancer agent in Colorectal cancer.
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http://dx.doi.org/10.7150/jca.44375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477418PMC
August 2020

Cascade-Targeting of Charge-Reversal and Disulfide Bonds Shielding for Efficient DOX Delivery of Multistage Sensitive MSNs-COS-SS-CMC.

Int J Nanomedicine 2020 17;15:6153-6165. Epub 2020 Aug 17.

School of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450001, People's Republic of China.

Background: Although pH and redox sensitiveness have been extensively investigated to improve therapeutic efficiency, the effect of disulfide bonds location and pH-triggered charge-reversal on cascade-targeting still need to be further evaluated in cancer treatment with multi-responsive nanoparticles.

Purpose: The aim of this study was to design multi-responsive [email protected], [email protected] and [email protected] and systematically investigate the effects of disulfide bonds location and charge-reversal on the cancer cell specificity, endocytosis mechanisms and antitumor efficiency.

Results: In vitro drug release rate of [email protected] in tumor environments was 7-fold higher than that under normal physiological conditions after 200 h. Furthermore, the fluorescence intensity of [email protected] and [email protected] was 1.9-fold and 1.3-fold higher than free DOX at pH 6.5 and 10 mM GSH. In addition, vesicular transport might be a factor that affects the uptake efficiency of [email protected] and [email protected] The clathrin-mediated endocytosis and endosomal escape of [email protected] enhanced cellular internalization and preserved highly controllable drug release into the perinuclear of HeLa cells. [email protected] exhibited a synergistic chemotherapy in preeminent tumor inhibition and less side effects of cardiotoxicity.

Conclusion: The cascade-targeting of charge-reversal and disulfide bonds shielding would be a highly personalized strategy for cervical cancer treatment.
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http://dx.doi.org/10.2147/IJN.S252769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443036PMC
November 2020

Novel Caffeic Acid Phenethyl Ester-Mortalin Antibody Nanoparticles Offer Enhanced Selective Cytotoxicity to Cancer Cells.

Cancers (Basel) 2020 Aug 21;12(9). Epub 2020 Aug 21.

AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba, Ibaraki 305-8565, Japan.

Caffeic acid phenethyl ester (CAPE) is a key bioactive ingredient of honeybee propolis and is claimed to have anticancer activity. Since mortalin, a hsp70 chaperone, is enriched in a cancerous cell surface, we recruited a unique cell internalizing anti-mortalin antibody (MotAb) to generate mortalin-targeting CAPE nanoparticles (CAPE-MotAb). Biophysical and biomolecular analyses revealed enhanced anticancer activity of CAPE-MotAb both in in vitro and in vivo assays. We demonstrate that CAPE-MotAb cause a stronger dose-dependent growth arrest/apoptosis of cancer cells through the downregulation of Cyclin D1-CDK4, phospho-Rb, PARP-1, and anti-apoptotic protein Bcl2. Concomitantly, a significant increase in the expression of p53, p21, and caspase cleavage was obtained only in CAPE-MotAb treated cells. We also demonstrate that CAPE-MotAb caused a remarkably enhanced downregulation of proteins critically involved in cell migration. In vivo tumor growth assays for subcutaneous xenografts in nude mice also revealed a significantly enhanced suppression of tumor growth in the treated group suggesting that these novel CAPE-MotAb nanoparticles may serve as a potent anticancer nanomedicine.
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http://dx.doi.org/10.3390/cancers12092370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564736PMC
August 2020

Effects of elevated nitrogen on the growth and geosmin productivity of Dolichospermum smithii.

Environ Sci Pollut Res Int 2021 Jan 15;28(1):177-184. Epub 2020 Aug 15.

National Institute of Public Health, 2-3-6 Minami Wako, Saitama, Japan.

Geosmin is one of the most common earthy-musty odor compounds, which is mainly produced by cyanobacteria in surface water. Nitrogen (N) is an important factor affecting the growth of cyanobacteria and its secondary metabolites production due to the eutrophication. In this study, we compared the effects of elevated N on the growth and geosmin productivity of Dolichospermum smithii NIES-824 (synonym Anabaena smithii NIES-824), aiming to better understand the mechanisms involved and give an important and fundamental knowledge to solve off-flavor problem. Results show that elevated N concentration promoted more chlorophyll a (Chl-a) production, whereas the geosmin synthesis decreased, revealing a possible competitive correlation between the Chl-a concentration and geosmin production of D. smithii NIES-824. The majority of geosmin (> 90%) was retained intracellularly during the 28 days of cultivation. The qRT-PCR analysis demonstrates that the expression level of the geosmin synthase gene (geoA) was constitutive and decreased at the higher N concentration during the exponential growth phase of cyanobacterial cells. Furthermore, the decrease of geoA expression during the decline phase suggested that geoA transcription was closely related to cell activity and isoprenoid productivity.
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http://dx.doi.org/10.1007/s11356-020-10429-4DOI Listing
January 2021
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