Publications by authors named "Zhenxing Zhang"

123 Publications

Biological Properties of Milk-Derived Extracellular Vesicles and Their Physiological Functions in Infant.

Front Cell Dev Biol 2021 25;9:693534. Epub 2021 Jun 25.

Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China.

Extracellular vesicles (EVs) are released by all cells under pathological and physiological conditions. EVs harbor various biomolecules, including protein, lipid, non-coding RNA, messenger RNA, and DNA. In 2007, mRNA and microRNA (miRNA) carried by EVs were found to have regulatory functions in recipient cells. The biological function of EVs has since then increasingly drawn interest. Breast milk, as the most important nutritional source for infants, contains EVs in large quantities. An increasing number of studies have provided the basis for the hypothesis associated with information transmission between mothers and infants via breast milk-derived EVs. Most studies on milk-derived EVs currently focus on miRNAs. Milk-derived EVs contain diverse miRNAs, which remain stable both and ; as such, they can be absorbed across different species. Further studies have confirmed that miRNAs derived from milk-derived EVs can resist the acidic environment and enzymatic hydrolysis of the digestive tract; moreover, they can be absorbed by intestinal cells in infants to perform physiological functions. miRNAs derived from milk EVs have been reported in the maturation of immune cells, regulation of immune response, formation of neuronal synapses, and development of metabolic diseases such as obesity and diabetes. This article reviews current status and advances in milk-derived EVs, including their history, biogenesis, molecular contents, and biological functions. The effects of milk-derived EVs on growth and development in both infants and adults were emphasized. Finally, the potential application and future challenges of milk-derived EVs were discussed, providing comprehensive understanding and new insight into milk-derived EVs.
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http://dx.doi.org/10.3389/fcell.2021.693534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267587PMC
June 2021

Integrated mRNA-seq and miRNA-seq analysis of goat fibroblasts response to strain M5-90.

PeerJ 2021 29;9:e11679. Epub 2021 Jun 29.

Hainan Key Lab of Tropical Animal Reproduction, Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, College of Animal Science and Technology, Hainan University, Hainan Key Lab of Tropical Animal Reproduction, Haikou, Hainan, China.

Brucellosis is a globally zoonotic bacterial disease of humans and various animals including goats, sheep, and cattle. M5-90, a live attenuated vaccine strain, has been widely used to prevent brucellosis in goats and sheep. However, the molecular mechanisms governing protective immunity response in non-professional phagocytes infected with M5-90 have not been fully investigated, especially in goats. In our research, goat fibroblasts were used as in vitro models to determine these mechanisms by transcriptome analysis. After incubating with M5-90 3 h, the infected goat fibroblasts were collected at 0 h, 4 h, 24 h, 48 h and 72 h for RNA-seq. The results indicated that there were totally 11,819 differentially expressed genes (DEGs) and 777 differentially expressed (DE) miRNAs found in experiment groups compared with the control groups (|log2(Foldchange)|≥1, FDR<0.05). GO and KEGG enrichment analyses revealed that down-regulated genes were involved in the riboflavin metabolism and positive regulation of IL-8 secretion pathway. The up-regulated genes were mainly involved in adaptive immunity, including TNF signaling pathway, MAPK signaling pathway and JAK/STAT pathway. Additionally, cytokine-cytokine receptor interaction, natural killer cell mediated cytotoxicity and toll-like receptor signaling pathway, which associated with innate immunity pathways, were also induced. Based on the Pearson correlation coefficients and prediction results of TargetScan and miRanda, the miRNA-mRNA networks of , and were constructed and verified in goat fibroblasts by qPCR, which demonstrated that goat fibroblasts displayed immunomodulatory properties. Our findings provide a deeper insight into the host miRNA-driven defense mechanism and reveal the transcriptome changes involved in the innate and adaptive immune response of the goats to infection.
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http://dx.doi.org/10.7717/peerj.11679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253117PMC
June 2021

Genome-Wide Differential Expression Profiling of Pulmonary circRNAs Associated With Immune Reaction to in Goats.

Front Vet Sci 2021 21;8:615405. Epub 2021 Jun 21.

Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, College of Animal Science and Technology, Hainan University, Haikou, China.

is a highly versatile pathogen that infects a wide range of animals, including goats, causing pneumonia and hemorrhagic septicemia. Circular RNA (circRNA) is a type of non-coding RNA that plays an important role in regulating cellular metabolism. However, whether and how circRNA is involved in regulating immune responses in the goat lung has not been reported. Thus, this study was designed to examine the function of circRNA in goats infected with . Goats were assigned into one of two groups: an uninfected control group (CK) and an infected group challenged with . Compared with the CK group, which remained healthy, the infected goats showed clinical signs of infection, including depression, cough, nasal discharge, and dyspnea, along with elevated body temperature and lesions in the lung. Whole-transcriptome sequencing and small RNA sequencing were then performed using lung samples from goats from each group. A total of 138 circRNA, 56 microRNAs (miRNA), and 2,673 messenger RNA (mRNA) molecules were significantly differentially expressed in the -infected group compared with the CK group. Randomly selected differentially expressed circRNA, miRNA, and mRNA molecules ( = 5 per group) were then validated by quantitative reverse-transcriptase polymerase chain reaction analysis. Gene ontology (GO) analysis of the source genes indicated that six immune-related terms were enriched among the differentially expressed cirRNA molecules, including inflammatory response, immune effector process, cell activation involved in immune response, cytokine-mediated signaling pathway, response to endogenous stimulus, and immune response. The corresponding circRNA molecules were then selected for construction of a competitive endogenous RNA network to identify networks that may be involved in the immune response to infection. The results indicated that HN01 may cause pneumonia and stimulate an immune response in goats via regulation of circRNA expression. This study presents the first comprehensive circRNA profile in response to infection in goats, thus, providing a basis for understanding the function of circRNA in the host immune response to infection.
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http://dx.doi.org/10.3389/fvets.2021.615405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256745PMC
June 2021

Anodic oxidation triggered divergent 1,2- and 1,4-group transfer reactions of β-hydroxycarboxylic acids enabled by electrochemical regulation.

Chem Sci 2020 Sep 28;11(44):12021-12028. Epub 2020 Sep 28.

Department of Energy and Resources Engineering, College of Engineering, Peking University Beijing 100871 China

We report a set of electrochemically regulated protocols for the divergent synthesis of ketones and β-keto esters from the same β-hydroxycarboxylic acid starting materials. Enabled by electrochemical control, the anodic oxidation of carboxylic acids proceeded in either a one-electron or a two-electron pathway, leading to a 1,4-aryl transfer or a semipinacol-type 1,2-group transfer product with excellent chemoselectivity. The 1,4-aryl transfer represents an unprecedented example of carbon-to-oxygen group transfer proceeding a radical mechanism. In contrast to previously reported radical group transfer reactions, this 1,4-group transfer process features the migration of electron-rich aryl substituents. Furthermore, with these chemoselective electrochemical oxidation protocols, a range of ketones and β-keto esters including those possessing a challenging-to-access medium-sized ring could be synthesized in excellent yields.
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http://dx.doi.org/10.1039/d0sc02386hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162457PMC
September 2020

A strangulated internal hernia beneath the left external iliac artery after radical hysterectomy with laparoscopic pelvic lymphadenectomy: a case report and literature review.

BMC Surg 2021 May 31;21(1):273. Epub 2021 May 31.

Department of Gastrointestinal Surgery, Shaoxing People's Hospital (Shaoxing Hospital of Zhejiang University), Shaoxing, 312000, China.

Background: Ileum obstruction due to internal hernia beneath external iliac artery after pelvic lymph node dissection (PLND) is extremely rare. We reported a case of acute strangulated internal hernia between the left external iliac artery and psoas major as late complication of laparoscopic hysterectomy with pelvic lymphadenectomy.

Case Presentation: A 46-year-old woman, who with histories of laparoscopic hysterectomy, bilateral salpingo-oophorectomy and PLND 9 years ago for the cervical malignant tumor, open appendectomy 18 years ago, visited our hospital complaining of aggravated left lower abdominal pain, bloating, nausea and vomiting from few hours ago. Left abdomen distention, tympanitic with rebound tenderness and muscular tension was detected during physical examinations. Accompanying with elevated inflammatory markers and mild intestinal dilatation showed in lab results and contrast-enhanced computed tomography (CT) respectively. After carefully reading the CT images, a small bowel was found between the left external iliac artery (EIA) and the psoas major, combined with the patient's surgical history, we suspected it might be internal hernia. Eventually, the emergency laparoscopic laparotomy confirmed our conjecture, the gap between the iliac vessels and the psoas major was closed with an absorbable suture, the patient was discharged on the fourth postoperative day.

Conclusion: Primary closure of peritoneal fissue maybe an effective measure to potentially prevent internal hernia. The choice of surgical approach for pelvic tumors still needs further exploration but faster diagnosis and immediate laparotomy might promise a better prognosis.
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http://dx.doi.org/10.1186/s12893-021-01249-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166092PMC
May 2021

Src family kinases involved in the differentiation of human preadipocytes.

Mol Cell Endocrinol 2021 08 14;533:111323. Epub 2021 May 14.

Nanjing Maternal and Child Health Medical Institute, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu 210004, China. Electronic address:

Background: Obesity is characterized by the excess accumulation of white adipose tissue (WAT). Src family kinases (SFKs) are non-receptor tyrosine kinases consisting of eight members (SRC, FYN, YES1, HCK, LCK, LYN, FGR and BLK) that have been studied extensively in mammalian cells. Although individual members in murine cells provide some clues that are associated with the regulation of adipogenesis, the specific role of this family in adipocyte differentiation has rarely been assessed, especially in human adipocytes.

Methods: Herein, we first explored the expression profiles of SFKs during human preadipocyte differentiation. Then, we used the pyrazolo-pyrimidinyl-amine compound PP1, a potent SFK inhibitor, to evaluate the function of SFKs during adipocyte differentiation. Furthermore, we adopted a loss-of-function strategy with siRNAs to determine the role of FGR in adipocyte differentiation.

Results: Here, we found that SRC, FYN, YES1, LYN and FGR were expressed in human preadipocytes and induced after the initiation of differentiation. Furthermore, the SFK inhibitor PP1 suppressed adipocyte differentiation. We also found that PP1 significantly suppressed the SFK activity in preadipocytes and decreased the expression of adipogenic genes in early and late differentiation. Given that FGR exhibited the most expression enhancement in mature adipocytes, we focused on FGR and found that its knockdown reduced lipid accumulation and adipogenic gene expression.

Conclusions: Collectively, these findings suggest that SFKs, especially FGR, are involved in the differentiation of human preadipocytes. Our results lay a foundation for further understanding the role of SFKs in adipocyte differentiation and provide new clues for anti-obesity therapies.
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http://dx.doi.org/10.1016/j.mce.2021.111323DOI Listing
August 2021

Helicobacter pylori induces gastric cancer via down-regulating miR-375 to inhibit dendritic cell maturation.

Helicobacter 2021 May 3:e12813. Epub 2021 May 3.

School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.

Background: Recent studies and clinical samples have demonstrated that Helicobacter pylori could induce the downregulation of miR-375 in the stomach and promote gastric carcinogenesis. However, whether the immune cells are affected by Helicobacter pylori due to the downregulation of miR-375 is unclear.

Materials And Methods: In this study, we constructed an overexpression and knockdown of miR-375 and Helicobacter pylori infection cell models in vitro. In addition, the maturity of dendritic cells (DCs) and the expression of IL-6, IL-10, and VEGF at the transcriptional and translational levels were analyzed. Changes in the JAK2-STAT3 signaling pathway were detected. In vivo, the number changes in CD4+ T and CD8+ T cells and the size changes of tumors via models of transplantable subcutaneous tumors were also analyzed.

Results: A cell model of Helicobacter pylori and gastric cancer was used to identify the expression of miR-375 and the maturity of dendritic cells. This study found that Helicobacter pylori could downregulate miR-375, which regulates the expression of cytokines IL-6, IL-10, and VEGF in the stomach. MiR-375 regulated the expression of cytokines IL-6, IL-10, and VEGF through the JAK2-STAT3 signaling pathway in vitro. In addition, we found that Helicobacter pylori regulates the maturation of dendritic cells through miR-375. These results were further verified in vivo, and miR-375 diminishes tumor size was also demonstrated. This study showed that immature DCs caused a decrease in the number of CD4+ and CD8+ T cells.

Conclusions: This study demonstrated that Helicobacter pylori can inhibit miRNA-375 expression in the stomach. Downregulated miR-375 activates the JAK2-STAT3 pathway. Activating the JAK2-STAT3 signaling pathway promotes the secretion of IL-6, IL-10, and VEGF, leading to immature differentiation of DCs and induction of gastric cancer.
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http://dx.doi.org/10.1111/hel.12813DOI Listing
May 2021

SOX9 in prostate cancer is upregulated by cancer-associated fibroblasts to promote tumor progression through HGF/c-Met-FRA1 signaling.

FEBS J 2021 Mar 11. Epub 2021 Mar 11.

Department of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, China.

Transcription factor SOX9 was a biomarker for prostate cancer (Pca) with poor prognosis. Nevertheless, the regulatory mechanism underlying SOX9 upregulation still remains unclear. Several cytokines have been reported to be involved in the regulation of SOX9, suggesting that cancer-associated fibroblasts (CAFs), one of the main sources of secreted factors in the tumor microenvironment (TME), may play a role in regulating SOX9 expression. Herein, an in vitro model of paracrine interaction between primary CAFs and Pca cells was applied to investigate the molecular mechanism of SOX9 upregulation during Pca progression. The regulatory axis was validated by in vivo experiments and The Cancer Genome Atlas data. Conditional medium of CAFs (CAF-CM) upregulated the expression of SOX9, which was mutually proved to be essential for CAF-induced tumor progression. Further analysis showed that hepatocyte growth factor (HGF) secreted by CAFs was responsible for SOX9 elevation in Pca cells, via the activation of c-Met signaling. Mechanistically, HGF/c-Met signaling specifically activated MEK1/2-ERK1/2 pathway, which induced phosphorylation and upregulation of FRA1, which then transcriptionally upregulated SOX9 by binding to the promoter of SOX9 gene. Moreover, we identified that HGF/c-Met-ERK1/2-FRA1-SOX9 axis was relatively conserved between human and mouse species by validating in mouse Pca cells. Our results reveal a novel insight into the molecular mechanism that SOX9 in Pca cells is promoted by CAFs through HGF/c-Met-ERK1/2-FRA1 axis. Furthermore, SOX9 may serve as an alternative marker for the activated HGF/c-Met signaling to enroll the optimal Pca patients for HGF/c-Met inhibition treatment, since it is much more stable and easier to detect.
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http://dx.doi.org/10.1111/febs.15816DOI Listing
March 2021

Hepatitis B-Induced IL8 Promotes Hepatocellular Carcinoma Venous Metastasis and Intrahepatic Treg Accumulation.

Cancer Res 2021 May 2;81(9):2386-2398. Epub 2021 Mar 2.

School of Life Sciences, University of Science and Technology of China, Hefei, China.

Hepatitis B-associated hepatocellular carcinoma (HCC) is often accompanied by severe vascular invasion and portal vein tumor thrombus, leading to a poor prognosis. However, the underlying mechanism of this disease remains obscure. In this study, we demonstrate that the hepatitis B virus (HBV)-encoded gene HBx induces high IL8 production through MEK-ERK signal activation, leading to enhanced endothelial permeability to facilitate tumor vascular invasion. In a vascular metastatic model using a tail vein injection in a transgenic mouse with selective expression of human CXCR1 in the endothelium, activation of the IL8-CXCR1 cascade by overexpression of IL8 in tumor cells dramatically enhanced liver metastasis. Mechanistically, IL8 selectively induced GARP-latent-TGFβ in liver sinusoidal endothelial cells and subsequently provoked preferential regulatory T-cell polarization to suppress antitumor immunity. Collectively, these findings reveal a hepatitis B-associated IL8-CXCR1 signaling axis that mediates vascular invasion and local microenvironmental immune escape of HCC to induce intrahepatic metastasis, which may serve as potential therapeutic targets for HBV-associated HCC. SIGNIFICANCE: This study identifies a hepatitis B-induced IL8/CXCR1/TGFβ signaling cascade that suppresses antitumor immunity and enhances metastasis in hepatocellular carcinoma, providing new potential targets for therapeutic intervention.
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http://dx.doi.org/10.1158/0008-5472.CAN-20-3453DOI Listing
May 2021

Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties.

Nat Commun 2021 01 18;12(1):423. Epub 2021 Jan 18.

State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, 712100, Yangling, Shaanxi, China.

Bacterial type VI secretion systems (T6SSs) inject toxic effectors into adjacent eukaryotic and prokaryotic cells. It is generally thought that this process requires physical contact between the two cells. Here, we provide evidence of contact-independent killing by a T6SS-secreted effector. We show that the pathogen Yersinia pseudotuberculosis uses a T6SS (T6SS-3) to secrete a nuclease effector that kills other bacteria in vitro and facilitates gut colonization in mice. The effector (Tce1) is a small protein that acts as a Ca- and Mg-dependent DNase, and its toxicity is inhibited by a cognate immunity protein, Tci1. As expected, T6SS-3 mediates canonical, contact-dependent killing by directly injecting Tce1 into adjacent cells. In addition, T6SS-3 also mediates killing of neighboring cells in the absence of cell-to-cell contact, by secreting Tce1 into the extracellular milieu. Efficient contact-independent entry of Tce1 into target cells requires proteins OmpF and BtuB in the outer membrane of target cells. The discovery of a contact-independent, long-range T6SS toxin delivery provides a new perspective for understanding the physiological roles of T6SS in competition. However, the mechanisms mediating contact-independent uptake of Tce1 by target cells remain unclear.
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http://dx.doi.org/10.1038/s41467-020-20726-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813860PMC
January 2021

Shelf-Stable Quantum-Dot Light-Emitting Diodes with High Operational Performance.

Adv Mater 2020 Dec 16;32(52):e2006178. Epub 2020 Nov 16.

Centre for Chemistry of High-Performance & Novel Materials, State Key Laboratory of Silicon Materials, Department of Chemistry, Zhejiang University, Hangzhou, 310027, China.

Quantum-dot light-emitting diodes (QLEDs) promise a new generation of high-performance, large-area, and cost-effective electroluminescent devices for both display and solid-state lighting technologies. However, a positive ageing process is generally required to improve device performance for state-of-the-art QLEDs. Here, it is revealed that the in situ reactions induced by organic acids in the commonly used encapsulation acrylic resin lead to positive ageing and, most importantly, the progression of in situ reactions inevitably results in negative ageing, i.e., deterioration of device performance after long-term shelf storage. In-depth mechanism studies focusing on the correlations between the in situ chemical reactions and the shelf-ageing behaviors of QLEDs inspire the design of an electron-transporting bilayer, which delivers both improved electrical conductivity and suppressed interfacial exciton quenching. This material innovation enables red QLEDs exhibiting neglectable changes of external quantum efficiency (>20.0%) and ultralong operational lifetime (T : 5500 h at 1000 nits) after storage for 180 days. This work provides design principles for oxide electron-transporting layers to realize shelf-stable and high-operational-performance QLEDs, representing a new starting point for both fundamental studies and practical applications.
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http://dx.doi.org/10.1002/adma.202006178DOI Listing
December 2020

Circ_0006528 Contributes to Paclitaxel Resistance of Breast Cancer Cells by Regulating miR-1299/CDK8 Axis.

Onco Targets Ther 2020 24;13:9497-9511. Epub 2020 Sep 24.

Department of Integrated Traditional Chinese and Western Medicine in Oncology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, People's Republic of China.

Background: Circular RNAs (circRNAs) have been reported to be involved in regulating the development of breast cancer. Paclitaxel (PTX) can be used for the chemotherapy of breast cancer. The study aimed to explore the role and mechanism of circ_0006528 in PTX-resistant breast cancer progression.

Methods: The levels of circ_0006528, microRNA-1299 (miR-1299) and cyclin-dependent kinase 8 () were measured by quantitative real-time polymerase chain reaction (qRT-PCR). RNase R treatment was used to confirm that the circ_0006528 was a circular RNA. PTX resistance and cell proliferation were determined by Cell counting kit-8 (CCK-8) assay. Cell apoptosis, migration and invasion were analyzed by flow cytometry and Transwell assays, respectively. The levels of all proteins were examined by Western blot. The interaction between circ_0006528 and miR-1299 or was predicted by online database confirmed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Xenograft mice model was constructed to reveal the role of circ_0006528 on tumor growth in vivo.

Results: Circ_0006528 was significantly up-regulated and miR-1299 was down-regulated in PTX-resistant breast cancer tissues and cells compared with control groups. CDK8 protein expression was dramatically upregulated in PTX-resistant breast cancer tissues and cells as compared to control groups. Loss-of-function experiments revealed that circ_0006528 knockdown decreased IC50 value of PTX and restrained proliferation, migration, invasion and autophagy, whereas induced apoptosis of PTX-resistant breast cancer cells in vitro. The inhibitory effects of sh-circ_0006528 on the progression of PTX-resistant breast cancer cells were reversed by decreasing miR-1299 or increasing expression. Furthermore, circ_0006528 could modulate expression by sponging miR-1299. Circ_0006528 silencing impeded the growth of PTX-resistant tumors by regulating miR-1299/ axis in vivo.

Conclusion: Circ_0006528 partially contributed to PTX resistance of breast cancer cells through up-regulating expression by sponging miR-1299.
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http://dx.doi.org/10.2147/OTT.S252886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522311PMC
September 2020

Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against .

Emerg Microbes Infect 2020 Dec;9(1):2289-2302

School of Life Science and Technology, China Pharmaceutical University, Nanjing, People's Republic of China.

Tissue-resident memory T (T) cells, located in the epithelium of most peripheral tissues, constitute the first-line defense against pathogen infections. Our previous study reported that gastric subserous layer (GSL) vaccination induced a "pool" of protective tissue-resident memory CD4+T (CD4+T) cells in the gastric epithelium. However, the mechanistic details how CD4+T cells form in the gastric epithelium are unknown. Here, our results suggested that the vaccine containing CCF in combination with Silk fibroin hydrogel (SF) broadened the distribution of gastric intraepithelial CD4+T cells. It was revealed that the gastric intraepithelial T cells were even more important than circulating memory T cells against infection by . It was also shown that gastric-infiltrating neutrophils were involved as indispensable mediators which secreted CXCL10 to chemoattract CXCR3+CD4+T cells into the gastric epithelium. Blocking of CXCR3 or neutrophils significantly decreased the number of gastric intraepithelial CD4+T cells due to reduced recruitment of CD4+T cells. This study demonstrated the protective efficacy of gastric CD4+T cells against infection, and highlighted the influence of neutrophils on gastric intraepithelial CD4+T cells formation.
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http://dx.doi.org/10.1080/22221751.2020.1830719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7594714PMC
December 2020

Levofloxacin: Is It Still Suitable as an Empirically used Antibiotic During the Perioperative Period of Flexible Ureteroscopic Lithotripsy? A Single-center Experience with 754 Patients.

Urol J 2020 Oct 1. Epub 2020 Oct 1.

Department of Urology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China.

Purpose: To determine the empirical usage of antibiotics and analyze the pathogen spectrum during the perioperative period of flexible ureteroscopic lithotripsy (FURSL) with a focus on levofloxacin.

Materials And Methods: This retrospective analysis included 754 patients who underwent FURSL successfully in our hospital from January 2015 to July 2019. All patients were indicated urine cultures and prescribed antibiotics during the perioperative period. Patients with negative preoperative urine cultures were divided into levofloxacin (LVXG) and non-levofloxacin groups (NLVXG) based on the empirical use of antibiotics. Operative time, the length of postoperative hospital stays and total hospital stays, total hospitalization costs, postoperative fever rate and removal rate of stones were compared. Patients with positive urine cultures were analyzed for  pathogen distribution and antibiotic resistance.

Results: In the empirical use of antibiotics among 541 cases with negative urine cultures, the prescription rate of levofloxacin was 68.95%. Compared to that in NLVXG, LVXG had a lower cost of antibiotics but higher postoperative fever rate and longer hospital stay. There were no significant differences in operative time, the total hospitalization costs and the removal rate of stones between the two groups. The top two common pathogens were Escherichia coli (36.11%) and Enterococcus faecalis (24.07%), with resistance rates of 74.36% and 71.15% to levofloxacin, respectively.

Conclusion: Levofloxacin might be no longer suitable as the first-line choice of clinical experience when performing FURSL in our center.
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http://dx.doi.org/10.22037/uj.v16i7.6033DOI Listing
October 2020

Boosting the Electrochemical Performance of Graphene-Based On-Chip Micro-Supercapacitors by Regulating the Functional Groups.

ACS Appl Mater Interfaces 2020 Sep 14;12(38):42933-42941. Epub 2020 Sep 14.

Key Laboratory for Magnetism and Magnetic Materials of the Ministry of Education, School of Physical Science and Technology, Lanzhou University, Lanzhou 730000, P. R. China.

The on-chip system-compatible power supply shows a high demand for the rapid development of miniaturization devices, such as wireless sensors, remote detecting devices, etc. Moreover, the ever-increasing trends of multifunctionalities and long-term working conditions of such devices raise a high-performance standard for the power supply. Herein, the high-performance electrochemical energy storage micro-supercapacitors (MSCs) are obtained with a metal current collector-free symmetric graphene-based planar structure, in which the functional group of graphene was regulated extensively via fully compatible microfabrication techniques of blue-violet (BV) laser exposure and air plasma treatment. BV laser exposure enhanced the electrical conductivity by reducing the substantial functional groups. Furthermore, the wettability and active sites are tuned by air plasma treatment, thus creating a slightly functional group onto the graphene surface. The resulting reduced graphene oxide (RGO) shows a very low resistance down to 27.2 Ω sq, ensuring its superb electron conductivity for fast electron transfer during the electrochemical reactions. The electrochemical performance measurements reveal an areal capacitance as high as 21.86 mF cm, which delivers a power density of 5 mW cm with an energy density of 2.49 μWh cm. Moreover, it shows superior long-term stability with 99% retention after 10 000 cycles, which is beyond that of most of the reported graphene-based all-solid-state MSCs.
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http://dx.doi.org/10.1021/acsami.0c11085DOI Listing
September 2020

Impacts of pollution, sex, and tide on the time allocations to behaviours of Uca arcuata in mangroves.

Sci Total Environ 2020 Nov 3;742:140609. Epub 2020 Jul 3.

Institute for Ecological Research and Pollution Control of Plateau Lakes, School of Ecology and Environmental Science, Yunnan University, Kunming, Yunnan 650500, China. Electronic address:

Fiddler crabs (Uca) are ecosystem engineers in coastal ecosystems. Many anthropogenic and natural factors can affect the time allocated to various behaviours in Uca. However, the behaviour of U. arcuata, a widely distributed fiddler crab in Asia, has not been studied in mainland China. Here, we used binoculars to record the time budget of ten behaviours of U. arcuata to investigate the potential effects of sex, tides, and pollution on these behaviours. We found that the crabs spent 42.3%, 27.0%, and 10.6% of their time on feeding, feeding while walking, and stationary respectively. The crabs spent <1.5% of their time on copulation and grooming. The total foraging time (feeding + feeding while walking) did not differ among the three polluted sites. However, crabs spent more time on feeding but less time on feeding while walking. The feeding rate and probability of burrowing and grooming decreased while the possibility of locomotion and stay in burrow increased with increasing nutrient concentration. Females spent 13.9% more time on feeding and fed 54.9% faster than males. Males had a higher tendency to grooming and combat while they were less likely to walk than females. Regarding to the influence of tide, fiddler crabs fed 11.2% faster at ebb tides than at flood tides, and they were more likely to walk and stay in burrows at flood tides than at ebb tides. Our results indicated that nutrient pollution had stronger impacts on the behaviours of crabs than sex and tide. In polluted mangroves, increasing nutrient concentration reduced the quantity of sediment processed by fiddler crabs due to their smaller feeding area, slower feeding rate, and reduced frequency of burrowing activities. These results imply that mitigating nutrient pollution in mangroves may benefit the restoration and management of coastal ecosystems through the enhanced engineering functions of fiddler crabs.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140609DOI Listing
November 2020

LncRNA PLAC2 upregulates miR-663 to downregulate TGF-β1 and suppress bladder cancer cell migration and invasion.

BMC Urol 2020 Jul 10;20(1):94. Epub 2020 Jul 10.

Department of Urology, The first affiliated hospital of Wannan Medical College, No. 2, Zheshan West Road, Jinghu District, Wuhu City, Anhui Province, 241001, P. R. China.

Background: The roles of lncRNA PLAC2 in bladder cancer (BC) were explored.

Methods: The expression of PLAC2 in two types of tissue of BC patients was detected by RT-qPCR and the expression data were compared by paired t test. The 56 patients were staged according to the AJCC criteria, and 12, 15, 15 and 14 cases were classified into stage I-IV, respectively. The expression of TGF-β1 and miR-663 in BC tissues were also detected by RT-qPCR experiments.

Results: Our data showed that the expression levels of PLAC2 were significantly lower in BC tissues than that in non-cancer tissues. The expression of PLAC2 was not affect by clinical stages and low expression levels of PLAC2 predicted lower survival rate. The expression of PLAC2 was positively correlated with miR-663 and inversely correlated with TGF-β1 in BC tissues. In BC cells, downregulated TGF-β1 and upregulated miR-663 were observed after the overexpression of PLAC2. Overexpression of PLAC2 also resulted in suppressed invasion and migration of BC cells. Overexpression of miR-663 resulted in downregulated TGF-β1 but did not affect the expression of PLAC2. Overexpression of TGF-β1 reduced the inhibitory effects of overexpression of PLAC2 and miR-663 on cell migration and invasion.

Conclusion: PLAC2 can upregulate miR-663 to downregulate TGF-β1 and suppress BC cell migration and invasion.
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http://dx.doi.org/10.1186/s12894-020-00663-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350696PMC
July 2020

The urinary β microglobulin-creatinine ratio is inversely associated with lumbar spine bone mineral density in the elderly Chinese males.

Arch Osteoporos 2020 06 18;15(1):90. Epub 2020 Jun 18.

Department of Nephrology, Huadong Hospital affiliated to Fudan University, No. 221 West Yan'an Road, Shanghai, 200040, People's Republic of China.

Renal tubule cells play a pivotal role in maintaining bone homeostasis. Hence, renal tubular function may be associated with bone mineral density. Our study found that urinary β microglobulin-creatinine ratio (UBCR) levels correlated negatively with lumbar spine bone mineral density (BMD) and T and Z values, and may be a marker for osteoporosis in Chinese elderly male adults.

Purpose: To study the association of UBCR levels with BMD and the predictive value of UBCR for osteoporosis in elderly Chinese male adults.

Methods: A cross-sectional study of 149 (65 to 85 years, 69.7 ± 4.6) Chinese male adults who underwent health checkups in Huadong Hospital in Shanghai China was conducted. BMD was measured by dual-energy X-ray absorptiometry. The clinical variables and BMD of the participants in the low UBCR group (B1, UBCR < 300 μg/g) and the high UBCR group (B2, UBCR ≥ 300 μg/g) were compared. Associations between UBCR with clinical variables and BMD were analyzed by Pearson's correlation coefficient and multiple regression analysis. BMD and T and Z values were compared between the B1 and B2 groups. The odds ratios (ORs) for dose-dependent increases in osteoporosis between B1 and B2 were analyzed by binary logistic regression analysis. The area under the receiver operating characteristic (ROC) curve was used to analyze the capacity of UBCR to predict osteoporosis.

Results: UBCR was significantly higher in the osteoporosis group. After adjusting for multiple confounders, UBCR levels correlated negatively with BMD and T and Z values of the lumbar spine. Lumbar spine BMD and T and Z values were significantly lower in the B2 UBCR group than in the B1 UBCR group. Compared with the B1 participants, the ORs for "osteoporosis" were 12.401 times higher in B2 participants (P = 0.005) by binary logistic regression analysis after adjusting for potential confounders. The UBCR index (cutoff = 362.48 μg/g) had a sensitivity of 78.6% and a specificity of 68.7% for identifying osteoporosis, with an area under the ROC curve of 0.760.

Conclusions: These results suggest that UBCR levels correlate negatively with lumbar spine BMD and T and Z values and may serve as a marker for osteoporosis in Chinese elderly male adults.
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http://dx.doi.org/10.1007/s11657-020-00764-xDOI Listing
June 2020

Perivascular Lymphocyte Clusters Induced by Gastric Subserous Layer Vaccination Mediate Optimal Immunity against Helicobacter through Facilitating Immune Cell Infiltration and Local Antibody Response.

J Immunol Res 2020 11;2020:1480281. Epub 2020 Jan 11.

School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.

Background: vaccination-induced local inflammatory response resulted in the establishment of a pool of tissue-resident memory T (T) cells and new vessels after the resolution of inflammation. T cells have received increasing attention; however, the role of new vessels in protective response is still unknown.

Materials And Methods: We performed the laparotomy to access the stomach and injected alum-based vaccine into the gastric subserous layer (GSL). At 28 days post vaccination, a parabiosis mouse model along with depletion of anti-CD90.2 antibody was employed to explore the function of perivascular lymphocyte clusters in recall responses. The composition of the gastric lymphocyte clusters was analyzed by immunofluorescence staining. Antibody responses were detected using ELISA. Gastric lymphocytes were analyzed using flow cytometry.

Results: GSL vaccination induced the formation of new vessels in the inflamed region. These new vessels were different from native vessels in that they were generally accompanied by perivascular lymphocyte clusters that mainly consisted of CD90-expressing cells. Additionally, histological analysis revealed the presence of CD4 and CD8 T cells in the perivascular lymphocyte clusters. Administration of a dose of an anti-CD90.2 antibody to GSL-vaccinated mice resolved these clusters. The efficacy of protection was compared in the parabiosis mice. Upon challenge, the presence of perivascular lymphocyte clusters was responsible for the fast recall response, as depletion of these clusters by CD90.2 antibody administration resulted in decreased expressions of VCAM-1, Madcam-1, and TNF-, as well as lower recruitment of proinflammatory immune cells, decreased antibody levels, and poor protection.

Conclusions: Our research demonstrates that vaccination-induced regional inflammatory response contributes to optimal recall response not only by establishing a CD4 T pool but also by creating an "expressway," i.e., perivascular lymphocyte cluster.
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http://dx.doi.org/10.1155/2020/1480281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201474PMC
March 2021

HpaR, the Repressor of Aromatic Compound Metabolism, Positively Regulates the Expression of T6SS4 to Resist Oxidative Stress in .

Front Microbiol 2020 17;11:705. Epub 2020 Apr 17.

State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, China.

HpaR, a MarR family transcriptional regulator, was first identified in W for its regulation of the operon. Little else is known regarding its functionality. Here, we report that in , HpaR negatively regulates the operon similar to in W. To investigate additional functions of HpaR, RNA sequencing was performed for both the wild-type and the Δ mutant, which revealed that the type VI secretion system (T6SS) was positively regulated by HpaR. T6SS4 is important for bacteria resisting environmental stress, especially oxidative stress. We demonstrate that HpaR facilitates bacteria resist oxidative stress by upregulating the expression of T6SS4 in . . HpaR is also involved in biofilm formation, antibiotic resistance, adhesion to eukaryotic cells, and virulence in mice. These results greatly expand our knowledge of the functionality of HpaR and reveal a new pathway that regulates T6SS4.
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http://dx.doi.org/10.3389/fmicb.2020.00705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180172PMC
April 2020

Growth response of greenhouse-produced muskmelon and tomato to sub-surface drip irrigation and soil aeration management factors.

BMC Plant Biol 2020 Apr 6;20(1):141. Epub 2020 Apr 6.

Key Laboratory of Vegetation Ecology, Ministry of Education, Institute of Grassland Science, Northeast Normal University, Changchun, 130024, Jilin Province, China.

Background: Hypoxia causes injury and yield loss. Soil aeration has been reported to accelerate the growth of plants and increase crop yield. The aim of this study was to examine growth response of greenhouse-produced muskmelon to 3 levels of sub-surface drip irrigation (I), 3 different installation depths of drip laterals in the soil (D), and 4 levels of supplemental soil aeration frequency (A). A fractional factorial experiment was designed to examine these treatment effects on marketable fresh fruit yield, leaf area index during 3 growth stages, and dry matter partitioning at harvest. In addition, we studied the response of fruit yield and dry matter of tomato to 2 levels of burial depths of subsurface tubing in combination with 3 frequency levels of soil aeration.

Results: Results showed that soil aeration can positively influence the yield, leaf area index, dry matter and irrigation use efficiency of the muskmelon (p < 0.05). The fruit yield of muskmelon and tomato were increased by 21.5 and 30.8% respectively with 1-d and 2-d aeration intervals compared with the no aeration treatment.

Conclusions: The results suggest that soil aeration can positively impact the plant root zone environment and more benefits can be obtained with aeration for both muskmelon and tomato plants.
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http://dx.doi.org/10.1186/s12870-020-02346-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137469PMC
April 2020

Health risk assessment of trace elements exposure through the soil-plant (maize)-human contamination pathway near a petrochemical industry complex, Northeast China.

Environ Pollut 2020 Aug 27;263(Pt A):114414. Epub 2020 Mar 27.

Key Laboratory of Vegetation Ecology, Ministry of Education, Institute of Grassland Science, Northeast Normal University, Changchun, 130024, China; State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, Northeast Normal University, Changchun, 130024, China. Electronic address:

The trace elements contamination of agricultural soils near petrochemical industry complexes is a concern due to the risk of accumulating in food systems and subsequently affecting human health. We measured representative trace elements (Cu, Ni, Cr, Pb, Zn, Pb, Hg and As) through the soil-plant (maize)-human contamination pathway near a petrochemical industry complexes in an agricultural region from September 20 to 28, 2016. We found that the soil was mildly to moderately polluted by multiple trace elements, which was also confirmed by the contamination factor and enrichment factor values. Cd (enrichment factor = 2.28), Cu (2.75), Zn (1.85) and Pb (1.70) should be given more attention and prioritized over the other trace elements due to their higher potential risks. Furthermore, the trace elements contamination in maize grains was lower than the corresponding limits. The sequence of the transfer coefficient values was Zn > Cd > Cu > Hg > Ni > As > Cr > Pb. Maize grain safety was threatened mainly by Zn, Cd and Cu. There was no risk to humans through soil ingestion, while a potential health risk from maize grain consumption existed. Children were more sensitive than adults to the non-carcinogenic risks of maize grain consumption. Trace element As was found to be the priority metal for risk control. For carcinogenic risk, adults were more sensitive than children; As, Cr and Cd were the priority metals for risk control, with CR values exceeding the risk threshold (1 × 10). Overall, strict, intensive monitoring, especially of Cr and Cd, and soil protection measures are needed to prevent any furthertrace elements contamination and to ensure food safety. This study also provides a reference for similar studies worldwide.
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http://dx.doi.org/10.1016/j.envpol.2020.114414DOI Listing
August 2020

Liberation enhancement and copper enrichment improvement for waste printed circuit boards by heating pretreatment.

Waste Manag 2020 Apr 24;106:145-154. Epub 2020 Mar 24.

Key Laboratory of Coal Processing and Efficient Utilization of Ministry of Education, China University of Mining & Technology, Xuzhou, Jiangsu 221116, China. Electronic address:

Crushing is the key part for the recycling technology of waste printed circuit boards (WPCBs). In this study, the breakage and liberation effects of WPCBs was improved by heat pretreatment technology before crushing (HPBC). Based on the test results, 200 °C was found as the safe temperature for the HPBC of WPCBs. The fracture mode, particle size distribution, and enrichment characteristics of WPCBs were systematically studied. The experimental results show that the HPBC changed the breakage mode from longitudinal fracture to horizontal fracture and improved the liberation of metal from non-metal components. During the crushing process, the increase in the heat pretreatment time (30-120 min) and temperature (100-200 °C) can improve the crushing effect of WPCBs and increase the content of crushing products of -0.3 mm by 3.16% and 5.64%, respectively. Compared to the non-metallic components, the metal components have ductility and are difficult to break into -0.3 mm during the crushing process. HPBC can promote copper enrichment to narrow grain size. In the 0.3-1 mm range, the content of copper increased from 47.87% to 57.61%, an increase by 9.74%. The initial enrichment of copper was achieved by adjusting the crushing time. The recovery rate of copper can reach 85.66%, and the enrichment rate is 1.74 when 0.3-2 mm breaking product is used as enrichment. Therefore, HPBC can effectively improve the crushing and liberation effect of WPCBs and improve the enrichment rate, and thus is an effective pretreatment method.
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http://dx.doi.org/10.1016/j.wasman.2020.03.023DOI Listing
April 2020

Astragalus polysaccharides inhibit ovarian cancer cell growth via microRNA-27a/FBXW7 signaling pathway.

Biosci Rep 2020 03;40(3)

Oncology Department of Integrative Medicine, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang 471009, Henan, China.

Astragalus polysaccharide (APS), a natural antioxidant found in Astragalus membranaceus emerging as a novel anticancer agent, exerts antiproliferative and pro-apoptotic activity in various cancer cell types, but its effect on ovarian cancer (OC) remains unknown. In the present study, we tried to elucidate the role and mechanism of APS in OC cells. Our results showed that APS treatment suppressed the proliferation and induced apoptosis in OC cells. Afterward, the microRNA (miRNA) profiles in APS-treated cells were determined by a microarray assay, and whether APS affected OV-90 cells through regulation of miRNA was determined. Among these aberrant miRNAs, miR-27a was selected for further study as its oncogenic roles in various human cancers. Moreover, we found overexpression of miR-27a reversed the antiproliferation and pro-apoptotic effects of APS on OC cells. F-box and WD-40 domain protein 7 (FBXW7), a classical tumor suppressor, was found directly targeted by miR-27a and its translation was suppressed by miR-27a in OC cells. Finally, it was also observed that knockdown of FBXW7 by si-FBXW7 reversed the tumor suppressive activity of APS in OC cells, which is similar to the effects of miR-27a overexpression. Our findings demonstrate that APS can suppress OC cell growth in vitro via miR-27a/FBXW7 axis, and this observation reveals the therapeutic potential of APS for treatment of OC.
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http://dx.doi.org/10.1042/BSR20193396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103584PMC
March 2020

Seasonal Risk Assessment of Water-Electricity Nexus Systems under Water Consumption Policy Constraint.

Environ Sci Technol 2020 04 12;54(7):3793-3802. Epub 2020 Mar 12.

Key Laboratory of Water Cycle and Related Land Surface Processes, Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China.

Previous studies have estimated power plant cooling water consumption based on the long-term average cooling water consumption intensity (WI: water consumption per unit of electricity generation) at an annual scale. However, the impacts of the seasonality of WI and streamflow on electricity generation are less well understood. In this study, a risk assessment method is developed to explore the seasonal risk of water-electricity nexus based on the Integrated Environmental Control Model, which can simulate variable WIs in response to daily weather conditions and avoid underestimation in WIs as well as nexus risk during dry seasons. Three indicators, reliability, maximum time to recovery, and total power generation loss, are proposed to quantify the seasonal nexus risk under water consumption policy constraint represented by the allowed maximum percentage of water consumption to streamflow. The applications of the method in two representative watersheds demonstrate that the nexus risk is highly seasonal and is greatly impacted by the seasonal variability of streamflow rather than annual average water resources conditions on which most previous studies are based. The nexus is found more risky in the watershed with almost double mean annual streamflow and greater streamflow variability, compared with the watershed with less streamflow variability.
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http://dx.doi.org/10.1021/acs.est.0c00171DOI Listing
April 2020

miR-146b-5p Plays a Critical Role in the Regulation of Autophagy in ∆per -Infected RAW264.7 Cells.

Biomed Res Int 2020 19;2020:1953242. Epub 2020 Jan 19.

1 Hainan Key Lab of Tropical Animal Reproduction and Breeding and Epidemic Disease Research, College of Animal Science and Technology, Hainan University, Haikou, China.

-caused brucellosis is one of the most widespread worldwide zoonoses. Lipopolysaccharide (LPS) of , which functions as pathogen-associated molecular patterns (PAMPs), is an important virulence factor that elicits protective antibodies. of . is involved in the biosynthesis of the O-side chain of LPS. Autophagy is a crucial element of the innate immune response against intracellular pathogens including . In this study, we observed that autophagy was inhibited in RAW264.7 cells infected with ∆per. And, a high-throughput array-based screen and qRT-PCR validation were performed to identify the differentially expressed miRNAs in RAW264.7 cells infected with M5-90 ∆per. The results suggested that , , , and were upregulated and was downregulated. During M5-90 ∆per infection, the increased expression of inhibited the autophagy activation in RAW264.7 cells. Using a bioinformatics approach, was predicted to be a potential target of . The results of a luciferase reporter assay indicated that directly targeted the 3'-UTR of , and the interaction between and the 3'-UTR of was sequence-specific. High-throughput RNA-Seq-based screening was performed to identify differentially expressed genes in Tbc1d14-expressing RAW264.7 cells, and these were validated by qRT-PCR. Among the differentially expressed genes, four autophagy associated genes, 1 (), (), (), and (), were obtained. Our findings provide important insights into the functional mechanism of LPS of .
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http://dx.doi.org/10.1155/2020/1953242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995328PMC
November 2020

LncRNA HOTAIR promotes the invasion and metastasis of oral squamous cell carcinoma through metastasis-associated gene 2.

Mol Carcinog 2020 04 28;59(4):353-364. Epub 2020 Jan 28.

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu, China.

Despite therapeutic advancements, there has been little improvement in the survival status of patients with oral squamous cell carcinoma (OSCC). HOX antisense intergenic RNA (HOTAIR) has been shown to be dysregulated in several cancer types. However, the roles of HOTAIR in OSCC remain largely unknown. In this study, we investigated the association of HOTAIR expression with clinicopathological features in OSCC patients and the crucial roles of HOTAIR in the modulation of tumor progression. Our results showed that HOTAIR was highly expressed both in OSCC tissue samples and cell lines compared with corresponding normal oral mucosa tissues and human oral keratinocytes. Its overexpression was positively correlated with TNM (tumor-node-metastases) stage, histological grade, and regional lymph node metastasis. The knockdown of HOTAIR by short hairpin RNA significantly decreased the migration, invasion, and epithelial-mesenchymal transition of OSCC cells in vitro. Moreover, there was a negative correlation between HOTAIR and microRNA-326 expression in OSCC tissue samples and cell lines. Luciferase reporter and loss-of-function assays revealed that HOTAIR acted as a competitive endogenous RNA effectively sponging miR-326, thereby regulating the derepression of metastasis-associated gene 2 (MTA2). Finally, the expression and clinical significance of MTA2 were analyzed in another cohort of OSCC tissue samples. High MTA2 expression was significantly correlated with clinicopathological features of advanced OSCC and poor prognosis for patients with OSCC. Collectively, HOTAIR overexpression promoted the progression of OSCC. The HOTAIR-miR-326-MTA2 axis may contribute to a better understanding of OSCC pathogenesis and be a potential therapeutic target for OSCC.
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http://dx.doi.org/10.1002/mc.23159DOI Listing
April 2020

Moderate oxygen-deficient Fe(III) oxide nanoplates for high performance symmetric supercapacitors.

J Colloid Interface Sci 2020 Apr 17;565:458-464. Epub 2020 Jan 17.

Key Laboratory for Magnetism and Magnetic Materials of the Ministry of Education, Key Laboratory of Special Function Materials and Structure Design of the Ministry of Education, School of Physical Science and Technology, Lanzhou University, Lanzhou 730000, China. Electronic address:

As a promising anode material for supercapacitors, FeO has been widely studied but still face the problem of low conductivity. Inducing oxygen vacancy (Vo) into FeO is a widely used approach to tune the conductivity to enhance its capacitive performance, but there is little research on the influence of Vo content. Herein, we report the effect of Vo in FeO nanoplates with various content. We tuned the Vo content by annealing at 200-500 °C. XPS and EPR were taken to characterize the Vo content, ranging from 11% to 26%. Electrochemical results show that FO-300 with 17% Vo has the highest capacity of 301 mAh g, and the capacity of the highest Vo content's (26% Vo) is only 107 mAh g. The symmetric supercapacitor based on FO-300 shows a considerably high energy density of 58.5 Wh kg at a power density of 9.32 kW kg and remains 84.6% after 12,000 cycles.
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http://dx.doi.org/10.1016/j.jcis.2020.01.060DOI Listing
April 2020

2.6 V aqueous symmetric supercapacitors based on phosphorus-doped TiO nanotube arrays.

Dalton Trans 2020 Feb 23;49(6):1785-1793. Epub 2020 Jan 23.

School of Physical Science and Technology, Lanzhou University, Lanzhou 730000, China. and Key Laboratory for Magnetism and Magnetic Materials of the Ministry of Education, Lanzhou University, Lanzhou 730000, China.

Increasing the voltage window of an electrode material is effective for improving the energy density of aqueous symmetric supercapacitors. Herein, a novel aqueous symmetric supercapacitor equipped with a high cell voltage window of 2.6 V was assembled by P-doped TiO nanotube arrays on a Ti sheet. The arrays exhibit a wide potential range of about 1.2 V as the cathode, and a stable wide potential range of 1.4 V as the anode was also obtained. These wide potential windows in the cathode and anode render the symmetric supercapacitor with a very large working voltage window reaching 2.6 V, and thus a high volumetric energy density (1.65 mW h cm). These results suggest that P-doped TiO nanotube arrays can be promising candidates for energy storage devices.
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http://dx.doi.org/10.1039/c9dt04316kDOI Listing
February 2020
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