Publications by authors named "Zhenqian Zhang"

25 Publications

  • Page 1 of 1

Treatment of the saline-alkali soil with acidic corn stalk biochar and its effect on the sorghum yield in western Songnen Plain.

Sci Total Environ 2021 Jul 21;797:149190. Epub 2021 Jul 21.

College of Chemistry, Jilin University, Changchun 130012, China; Weihai Institute for Bionics-Jilin University, Weihai 264400, China. Electronic address:

Due to biochar could improve the physical and chemical properties of soil and promote crop growth, it is widely used in soil remediation, especially in saline soil. However, it is rarely studied of the application of acidic biochar in saline-alkali land. A field experiment with acidic corn stalk biochar (ACSBC) as a soil amendment was carried out in the western Songnen Plain of China. ACSBC (0, 0.15, 0.3, 0.45, 0.6, 0.75, 1, 6 and 15 t ha) was added to the topsoil to evaluate the combined effects on soil and sorghum yield. During the seeding and harvest period, the content of soil water, nutrient elements, cation exchange capacity (CEC), organic matter (OM), soluble cations (K, Ca, Mg) increased, Na content showed opposite trend. However, soil pH decreased averagely with 0.3 and 1.0 during the seeding and harvest period respectively, salinity decreased with 19.37% and 18.14%, exchange sodium percentage (ESP) decreased with 37.08% and 37.04%. The sorghum yield increased 32.98% averagely, significantly by 51.37% and 47.33% with the 0.6 and 1 t ha of ACSBC treatments respectively. These experimental results show that proper application of ACSBC in saline-alkali soil can effectively improve soil properties and increase sorghum yield.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149190DOI Listing
July 2021

Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury.

Nat Commun 2021 05 17;12(1):2863. Epub 2021 May 17.

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

During injury, monocytes are recruited from the circulation to inflamed tissues and differentiate locally into mature macrophages, with prior reports showing that cavity macrophages of the peritoneum and pericardium invade deeply into the respective organs to promote repair. Here we report a dual recombinase-mediated genetic system designed to trace cavity macrophages in vivo by intersectional detection of two characteristic markers. Lineage tracing with this method shows accumulation of cavity macrophages during lung and liver injury on the surface of visceral organs without penetration into the parenchyma. Additional data suggest that these peritoneal or pleural cavity macrophages do not contribute to tissue repair and regeneration. Our in vivo genetic targeting approach thus provides a reliable method to identify and characterize cavity macrophages during their development and in tissue repair and regeneration, and distinguishes these cells from other lineages.
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http://dx.doi.org/10.1038/s41467-021-23197-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129080PMC
May 2021

Tonoplast-associated calcium signaling regulates manganese homeostasis in Arabidopsis.

Mol Plant 2021 05 4;14(5):805-819. Epub 2021 Mar 4.

State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China. Electronic address:

Manganese (Mn) is an essential micronutrient in plants. However, excessive Mn absorption in acidic and waterlogged soils can lead to Mn toxicity. Despite their essential roles in Mn homeostasis, transcriptional and post-transcriptional modifications of Mn transporters remain poorly understood. Here, we demonstrated that high-Mn stress induces an obvious Ca signature in Arabidopsis. We identified four calcium-dependent protein kinases, CPK4/5/6/11, that interact with the tonoplast-localized Mn and iron (Fe) transporter MTP8 in vitro and in vivo. The cpk4/5/6/11 quadruple mutant displayed a dramatic high-Mn-sensitive phenotype similar to that of the mtp8 mutant. CPKs phosphorylated the N-terminal domain of MTP8 primarily at the Ser31 and Ser32 residues. Transport assays combined with multiple physiological experiments on phospho-dead variant MTP8 and phospho-mimetic variant MTP8 plants under different Mn and Fe conditions suggested that Ser31 and Ser32 are crucial for MTP8 function. In addition, genetic analysis showed that CPKs functioned upstream of MTP8. In summary, we identified a tonoplast-associated calcium signaling cascade that orchestrates Mn homeostasis and links Mn toxicity, Ca signaling, and Mn transporters. These findings provide new insight into Mn homeostasis mechanisms and Ca signaling pathways in plants, providing potential targets for engineering heavy metal toxicity-tolerant plants.
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http://dx.doi.org/10.1016/j.molp.2021.03.003DOI Listing
May 2021

Proliferation tracing reveals regional hepatocyte generation in liver homeostasis and repair.

Science 2021 02;371(6532)

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

Organ homeostasis is orchestrated by time- and spatially restricted cell proliferation. Studies identifying cells with superior proliferative capacities often rely on the lineage tracing of a subset of cell populations, which introduces a potential selective bias. In this work, we developed a genetic system [proliferation tracer (ProTracer)] by incorporating dual recombinases to seamlessly record the proliferation events of entire cell populations over time in multiple organs. In the mouse liver, ProTracer revealed more hepatocyte proliferation in distinct zones during liver homeostasis, injury repair, and regrowth. Clonal analysis showed that most of the hepatocytes labeled by ProTracer had undergone cell division. By genetically recording proliferation events of entire cell populations, ProTracer enables the unbiased detection of specific cellular compartments with enhanced regenerative capacities.
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http://dx.doi.org/10.1126/science.abc4346DOI Listing
February 2021

A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting.

Cell Stem Cell 2021 06 9;28(6):1160-1176.e7. Epub 2021 Feb 9.

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China; School of Life Science and Technology, ShanghaiTech University, 100 Haike Road, Shanghai 201210, China; School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:

The use of the dual recombinase-mediated intersectional genetic approach involving Cre-loxP and Dre-rox has significantly enhanced the precision of in vivo lineage tracing, as well as gene manipulation. However, this approach is limited by the small number of Dre recombinase driver constructs available. Here, we developed more than 70 new intersectional drivers to better target diverse cell lineages. To highlight their applicability, we used these new tools to study the in vivo adipogenic fate of perivascular progenitors, which revealed that PDGFRa but not PDGFRaPDGFRb perivascular cells are the endogenous progenitors of adult adipocytes. In addition to lineage tracing, we used members of this new suite of drivers to more specifically knock out genes in complex tissues, such as white adipocytes and lymphatic vessels, that heretofore cannot be selectively targeted by conventional Cre drivers alone. In summary, these new transgenic tools expand the intersectional genetic approach while enhancing its precision.
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http://dx.doi.org/10.1016/j.stem.2021.01.007DOI Listing
June 2021

Insight of the Influence of Magnetic-Field Direction on Magneto-Plasmonic Interfaces for Tuning Photocatalytical Performance of Semiconductors.

J Phys Chem Lett 2020 Nov 10;11(22):9931-9937. Epub 2020 Nov 10.

College of Sciences, Northeastern University, Shenyang 110004, China.

Boosting photocatalytic performance via external fields is an alternative and effective solution for improving the application performance of existing photocatalysts. Herein, using α-FeO-decorated TiO nanotube arrays as a model, we demonstrate the influence of magnetic field (MF)-direction on the photogenerated charge-carrier transfer behavior at plasmonic metal/semiconductor interfaces. For the first time, the photocatalytic activity is also found to correlate with the plasmonic metal species while applying an external MF. As verified by first-principles calculations, the spin-orbit coupling of metal contributes to the charge-carrier transfer. To highlight the anisotropic MF-tuning effect in practical applications, the as-prepared architecture is applied for photocatalysis-triggered drug delivery. The delivery rate can be remarkably accelerated by ∼38% under a tiny MF (0.4 T) with the proper direction. The findings in this research may provide new insight into designing semiconductor architectures for boosting the photocatalytical performance in an external MF.
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http://dx.doi.org/10.1021/acs.jpclett.0c02927DOI Listing
November 2020

Simultaneous quantitative assessment of two distinct cell lineages with a nuclear-localized dual genetic reporter.

J Mol Cell Cardiol 2020 09 12;146:60-68. Epub 2020 Jul 12.

The State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China; School of Life Science and Technology, Shanghai Tech University, Shanghai 201210, China; School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China. Electronic address:

Genetic lineage tracing has been widely used for studying in vivo cell fate plasticity during embryogenesis, tissue homeostasis, and disease development. Recent applications with multiple site-specific recombinases have been used in complex and sophisticated genetic fate mapping studies. However, the previous multicolor reporters for dual recombinases had limitations of precise in situ quantification of cell number, which is mainly due to the intermingling of cells in condensed tissues. Here, we generated a dual recombinase-mediated nuclear-localized GFP and tdTomato reporter line, which enables clear, simultaneous quantification of two distinct cell lineages in vivo. Combining this dual genetic reporter with Tbx18-Cre and Cdh5-Dre lines, which genetically trace epicardial and endothelial cells, respectively, we obtained high-resolution images for the anatomic distribution of the descendants of these two distinct cell lineages in the valve mesenchyme during development, remodeling, and maturation stages. This new dual genetic reporter is expected to facilitate fate tracing of two cell lineages and their objective quantification in vivo.
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http://dx.doi.org/10.1016/j.yjmcc.2020.07.002DOI Listing
September 2020

Genetic Fate Mapping of Transient Cell Fate Reveals N-Cadherin Activity and Function in Tumor Metastasis.

Dev Cell 2020 09 14;54(5):593-607.e5. Epub 2020 Jul 14.

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China; Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou 510632, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China. Electronic address:

Genetic lineage tracing unravels cell fate and plasticity in development, tissue homeostasis, and diseases. However, it remains technically challenging to trace temporary or transient cell fate, such as epithelial-to-mesenchymal transition (EMT) in tumor metastasis. Here, we generated a genetic fate-mapping system for temporally seamless tracing of transient cell fate. Highlighting its immediate application, we used it to study EMT gene activity from the local primary tumor to a distant metastatic site in vivo. In a spontaneous breast-to-lung metastasis model, we found that primary tumor cells activated vimentin and N-cadherin in situ, but only N-cadherin was activated and functionally required during metastasis. Tumor cells that have ever expressed N-cadherin constituted the majority of metastases in lungs, and functional deletion of N-cad significantly reduced metastasis. The seamless genetic recording system described here provides an alternative way for understanding transient cell fate and plasticity in biological processes.
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http://dx.doi.org/10.1016/j.devcel.2020.06.021DOI Listing
September 2020

Structural and cryogenic magnetic properties of rare earth rich RECoIn (RE = Gd, Dy and Ho) intermetallic compounds.

Dalton Trans 2020 Jul 18;49(25):8764-8773. Epub 2020 Jun 18.

Key Laboratory of Electromagnetic Processing of Materials (Ministry of Education), Northeastern University, Shenyang 110819, China.

The crystal structure, magnetic properties and magnetocaloric performances of rare earth rich RECoIn (RE = Gd, Dy and Ho) intermetallic compounds are investigated systematically in this work. All compounds in this system crystallize in the orthorhombic NdPdIn-type structure with the Cmmm space group. The stacks of alternate RE and Co/In atomic layers with z = 0, 1 and z = 1/2 along the z-axis constitute the crystal structure. These compounds belong to the REMX family with x = 9 and y = 2, and the ratio of the AlB-type and CsCl-type fragments in a unit cell is y : x, i.e. 2 : 9. The characteristic of multiple magnetic phase transition is revealed with a low magnetic flux density μH of 0.1 T for the present compounds. The ferromagnetic (FM) to paramagnetic (PM) phase transitions of the present compounds around their respective Curie temperatures (T) are all second order phase transitions (SOPTs). Around the T of 86, 37 and 20 K for GdCoIn, DyCoIn, and HoCoIn with a magnetic flux density change ΔμH of 0-7 T, the values of the maximum magnetic entropy change (-ΔS) and temperature averaged entropy change (TEC) with 3 K span are 10.95 and 10.93 J kg K for GdCoIn, 4.66 and 4.64 J kg K for DyCoIn, and 12.29 and 12.09 J kg K for HoCoIn, respectively. The corresponding values of relative cooling power (RCP) and refrigerant capacity (RC) are 538.1 and 405.9 J kg for GdCoIn, 213.9 and 165.9 J kg for DyCoIn, and 475.2 and 357.4 J kg for HoCoIn, respectively.
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http://dx.doi.org/10.1039/d0dt01212bDOI Listing
July 2020

GWAS and co-expression network combination uncovers multigenes with close linkage effects on the oleic acid content accumulation in Brassica napus.

BMC Genomics 2020 Apr 23;21(1):320. Epub 2020 Apr 23.

Collaborative Innovation Center of Grain and Oil Crops in South China, Hunan Agricultural University, Changsha, 410128, China.

Background: Strong artificial and natural selection causes the formation of highly conserved haplotypes that harbor agronomically important genes. GWAS combination with haplotype analysis has evolved as an effective method to dissect the genetic architecture of complex traits in crop species.

Results: We used the 60 K Brassica Infinium SNP array to perform a genome-wide analysis of haplotype blocks associated with oleic acid (C18:1) in rapeseed. Six haplotype regions were identified as significantly associated with oleic acid (C18:1) that mapped to chromosomes A02, A07, A08, C01, C02, and C03. Additionally, whole-genome sequencing of 50 rapeseed accessions revealed three genes (BnmtACP2-A02, BnABCI13-A02 and BnECI1-A02) in the A02 chromosome haplotype region and two genes (BnFAD8-C02 and BnSDP1-C02) in the C02 chromosome haplotype region that were closely linked to oleic acid content phenotypic variation. Moreover, the co-expression network analysis uncovered candidate genes from these two different haplotype regions with potential regulatory interrelationships with oleic acid content accumulation.

Conclusions: Our results suggest that several candidate genes are closely linked, which provides us with an opportunity to develop functional haplotype markers for the improvement of the oleic acid content in rapeseed.
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http://dx.doi.org/10.1186/s12864-020-6711-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181522PMC
April 2020

Structural and cryogenic magnetic properties of RENiIn (RE = Pr, Nd, Dy and Ho) compounds.

Dalton Trans 2019 Dec 26;48(48):17792-17799. Epub 2019 Nov 26.

Key Laboratory of Electromagnetic Processing of Materials (Ministry of Education), Northeastern University, Shenyang 110819, P. R. China. and Institute of Advanced Magnetic Materials, Hangzhou Dianzi University, Hangzhou 310018, P. R. China.

The crystal structures, magnetic properties and magneto-caloric effects (MCEs) of RENiIn (RE = Pr, Nd, Dy and Ho) compounds were investigated. The results indicate that PrNiIn and NdNiIn compounds have a tetragonal MoFeB-type structure belonging to the P4/mbm space group and undergo a second-order paramagnetic to ferromagnetic (PM to FM) transition at a Curie temperature (T) of 7.5 and 10.5 K, respectively, whereas DyNiIn and HoNiIn compounds have an orthorhombic MnAlB-type structure belonging to the space group Cmmm and possess a magnetic transition from PM to antiferromagnetic (AFM) at a Néel temperature T of 19 and 10.5 K together with a first-order field induced metamagnetic transition, respectively. Moreover, an additional magnetic transition at a lower temperature of around 5.5 K is detected for the HoNiIn compound. A considerable reversible magneto-caloric effect is observed accompanying the magnetic phase transition, and the maximum values of the magnetic entropy change (-ΔS) of the present RENiIn series compounds are determined to be 9.3, 11.5, 6.4 and 11.5 J kg K with a magnetic field change (ΔH) of up to 0-5 T for RE = Pr, Nd, Dy and Ho, respectively.
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http://dx.doi.org/10.1039/c9dt03245bDOI Listing
December 2019

Reassessment of c-Kit Cells for Cardiomyocyte Contribution in Adult Heart.

Circulation 2019 07 8;140(2):164-166. Epub 2019 Jul 8.

State Key Laboratory of Cell Biology, Chinese Academy of Sciences Center for Excellence on Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, China (L.H., M.H., Z.Z., Y.L., X.H., X.L., W.P., H.Z., B.Z.).

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http://dx.doi.org/10.1161/CIRCULATIONAHA.119.039909DOI Listing
July 2019

Corrigendum to "The CCCH-type transcription factor BnZFP1 is a positive regulator to control oleic acid levels through the expression of diacylglycerol O-acyltransferase 1 gene in Brassica napus" [Plant Physiol. Biochem. 132 (November 2018) 633-640].

Plant Physiol Biochem 2019 02 27;135:611. Epub 2018 Nov 27.

Key Laboratory of Oil Crop Biology of Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, Hubei, 430062, China; The National Oil Crops Improvement Center, Hunan Agricultural University, Changsha, Hunan, 410128, China; Hunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, Hunan, 410128, China. Electronic address:

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http://dx.doi.org/10.1016/j.plaphy.2018.10.039DOI Listing
February 2019

Corrigendum "The CCCH-type transcription factor BnZFP1 is a positive regulator to control oleic acid levels through the expression of diacylglycerol O-acyltransferase 1 gene in Brassica napus" [Plant Physiology and Biochemistry Volume 132, November 2018, Pages 633-640].

Plant Physiol Biochem 2018 12;133:158

Key Laboratory of Oil Crop Biology of Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, China; The National Oil Crops Improvement Center, Hunan Agricultural University, Changsha, Hunan, 410128, China; Hunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, Hunan, 410128, China. Electronic address:

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http://dx.doi.org/10.1016/j.plaphy.2018.11.008DOI Listing
December 2018

The CCCH-type transcription factor BnZFP1 is a positive regulator to control oleic acid levels through the expression of diacylglycerol O-acyltransferase 1 gene in Brassica napus.

Plant Physiol Biochem 2018 Nov 11;132:633-640. Epub 2018 Oct 11.

Key Laboratory of Oil Crop Biology of Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, China; The National Oil Crops Improvement Center, Hunan Agricultural University, Changsha, Hunan, 410128, China; Hunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, Changsha, Hunan, 410128, China. Electronic address:

In China, the high-oleic acid rapeseed has an oil content of ∼42% and oleic acid (18:1) content of ∼80%. Compared to ordinary rapeseed, high-oleic acid rapeseed has higher levels of monounsaturated fatty acids and lower levels of saturated fatty acid and polyunsaturated fatty acids, and thus is of high nutritional and health value. In addition, high-oleic acid rapeseed oil imparts cardiovascular protective effects. Based on these properties, high-oleic acid oil crops have been extensively investigated and cultivated. We previously identified a CCCH-type transcription factor (BnZFP1, GenBank accession number XM_013796508) that is associated with high oleic acid traits from a Brassica napus subtractive hybridization library. In the present study, we overexpressed and silenced the BnZFP1 gene of B. napus. BnZFP1-overexpressing plants exhibited an 18.8% increase in oleic acid levels and a 3.8% increase in oil content. However, BNZFP1-silenced plants showed a 4.5% decrease in oleic acid levels, whereas no significant change in oil content was observed. Microarray and pull-down assays indicated that BnZFP1 has a total of thirty potential target genes. Further analysis and validation of one of the potential target genes, namely, diacylglycerol O-acyltransferases 1 (DGAT1) gene, indicated that it is positively regulated by BnZFP1. We also observed a correlation between elevated DGAT1 gene expression levels and higher oil content and oleic acid levels in rapeseed.
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http://dx.doi.org/10.1016/j.plaphy.2018.10.011DOI Listing
November 2018

Nitrogen uptake and transfer in a soybean/maize intercropping system in the karst region of southwest China.

Ecol Evol 2017 10 10;7(20):8419-8426. Epub 2017 Sep 10.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi University Nanning China.

Nitrogen (N) deficiency occurs in over 80% of karst soil of southwest China, which restricts regional agricultural production. To test whether N fixed by legumes becomes available to nonfixing companion species, N fluxes between soybean and maize under no, partial, and total restriction of root contact were measured on a karst site in southwest China. N content and its transfer between soybean and maize intercrops were explored in a 2-year plot experiment, with N movement between crops monitored using N isotopes. Mesh barrier (30 μm) and no restrictions barrier root separation increased N uptake of maize by 1.28%-3.45% and 3.2%-3.45%, respectively. N uptake by soybean with no restrictions root separation was 1.23 and 1.56 times higher than that by mesh and solid barriers, respectively. In the unrestricted root condition, N transfer from soybean to maize in no restrictions barrier was 2.34-3.02 mg higher than that of mesh barrier. Therefore, it was implied that soybean/maize intercropping could improve N uptake and transfer efficiently in the karst region of southwest China.
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http://dx.doi.org/10.1002/ece3.3295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648690PMC
October 2017

Alizarin Yellow R (AYR) as compatible stabilizer for miniemulsion polymerization.

J Colloid Interface Sci 2017 Dec 3;507:337-343. Epub 2017 Aug 3.

Institute of Organic Chemistry III - Macromolecular Chemistry and Organic Materials, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany. Electronic address: http://www.uni-ulm.de/nawi/institut-fuer-organische-chemie-iii.html.

Hypothesis: Many solid particles have been used in Pickering stabilized (mini)emulsions. Stabilizing "particles" can be also formed in situ e.g. by aggregation of dye molecules as reported recently. Among the dyes sodium 2-hydroxy-5-[(E)-(4-nitrophenyl)diazenyl]benzoate (Alizarin Yellow R, (AYR)) is one of the best stabilizers. It is assumed to act as sole stabilizer also in heterophase polymerizations and offers a great potential for applications.

Experiments: Aqueous solutions of AYR in varying concentrations (0.3, 0.5, 1.0, 1.5, 2.0mg/mL (dye/water)) were employed as continuous phase in direct miniemulsions. The oil phase comprised ethenylbenzene (styrene) and hexadecane. The effects of AYR concentration and ultrasonication time on size and distribution of the droplets were investigated. The miniemulsions were polymerized with a water-soluble azo-initiator (2,2'-azobis[n-(2-carboxyethyl)-2-methylpropionamidine] n-hydrate, VA-057) and conversion and kinetics were determined.

Findings: The AYR is successfully employed as stabilizer in Pickering-like miniemulsion polymerizations of styrene. The higher the AYR concentrations the more stable the miniemulsions, the smaller the droplet sizes and the narrower the distributions are, ranging from ca. 450 to 180nm and 0.38 to 0.15, respectively. The nucleation mechanism of the polymer particles could be revealed by the number ratio of droplets and particles and follows droplet nucleation. This is confirmed by polymerization kinetics, which is in accordance with classical miniemulsion polymerization, too.
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http://dx.doi.org/10.1016/j.jcis.2017.08.007DOI Listing
December 2017

A Lipid-Anchored NAC Transcription Factor Is Translocated into the Nucleus and Activates Expression during Drought Stress.

Plant Cell 2017 Jul 6;29(7):1748-1772. Epub 2017 Jul 6.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China

The plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) play a vital role in the response to drought stress. Here, we report a lipid-anchored NACsa TF in MfNACsa is an essential regulator of plant tolerance to drought stress, resulting in the differential expression of genes involved in oxidation reduction and lipid transport and localization. MfNACsa is associated with membranes under unstressed conditions and, more specifically, is targeted to the plasma membrane through -palmitoylation. However, a Cys-to-Ser mutation or inhibition of -palmitoylation results in MfNACsa retention in the endoplasmic reticulum/Golgi. Under drought stress, MfNACsa translocates to the nucleus through de--palmitoylation mediated by the thioesterase MtAPT1, as coexpression of APT1 results in the nuclear translocation of MfNACsa, whereas mutation of the catalytic site of APT1 results in colocalization with MfNACsa and membrane retention of MfNACsa. Specifically, the nuclear MfNACsa binds the glyoxalase I () promoter under drought stress, resulting in drought tolerance by maintaining the glutathione pool in a reduced state, and the process is dependent on the APT1-NACsa regulatory module. Our findings reveal a novel mechanism for the nuclear translocation of an -palmitoylated NAC in response to stress.
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http://dx.doi.org/10.1105/tpc.17.00044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559744PMC
July 2017

Genome-Wide Association Study Dissecting the Genetic Architecture Underlying the Branch Angle Trait in Rapeseed (Brassica napus L.).

Sci Rep 2016 09 20;6:33673. Epub 2016 Sep 20.

Huazhong Agricultural University, National Key Laboratory of Crop Genetic Improvement, National Sub-center of Rapeseed Improvement in Wuhan, Wuhan, 430070, China.

The rapeseed branch angle is an important morphological trait because an adequate branch angle enables more efficient light capture under high planting densities. Here, we report that the average angle of the five top branches provides a reliable representation of the average angle of all branches. Statistical analyses revealed a significantly positive correlation between the branch angle and multiple plant-type and yield-related traits. The 60 K Brassica Infinium(®) single nucleotide polymorphism (SNP) array was utilized to genotype an association panel with 520 diverse accessions. A genome-wide association study was performed to determine the genetic architecture of branch angle, and 56 loci were identified as being significantly associated with the branch angle trait via three models, including a robust, novel, nonparametric Anderson-Darling (A-D) test. Moreover, these loci explained 51.1% of the phenotypic variation when a simple additive model was applied. Within the linkage disequilibrium (LD) decay ranges of 53 loci, we observed plausible candidates orthologous to documented Arabidopsis genes, such as LAZY1, SGR2, SGR4, SGR8, SGR9, PIN3, PIN7, CRK5, TIR1, and APD7. These results provide insight into the genetic basis of the branch angle trait in rapeseed and might facilitate marker-based breeding for improvements in plant architecture.
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http://dx.doi.org/10.1038/srep33673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5028734PMC
September 2016

Opposing Control by Transcription Factors MYB61 and MYB3 Increases Freezing Tolerance by Relieving C-Repeat Binding Factor Suppression.

Plant Physiol 2016 10 30;172(2):1306-1323. Epub 2016 Aug 30.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.)

Cold acclimation is an important process by which plants respond to low temperature and enhance their winter hardiness. C-REPEAT BINDING FACTOR1 (CBF1), CBF2, and CBF3 genes were shown previously to participate in cold acclimation in Medicago truncatula In addition, MtCBF4 is transcriptionally induced by salt, drought, and cold stresses. We show here that MtCBF4, shown previously to enhance drought and salt tolerance, also positively regulates cold acclimation and freezing tolerance. To identify molecular factors acting upstream and downstream of the MtCBF4 transcription factor (TF) in cold responses, we first identified genes that are differentially regulated upon MtCBF4 overexpression using RNAseq Digital Gene Expression Profiling. Among these, we showed that MtCBF4 directly activates the transcription of the COLD ACCLIMATION SPECIFIC15 (MtCAS15) gene. To gain insights into how MtCBF4 is transcriptionally regulated in response to cold, an R2R3-MYB TF, MtMYB3, was identified based on a yeast one-hybrid screen as binding directly to MYB cis-elements in the MtCBF4 promoter, leading to the inhibition of MtCBF4 expression. In addition, another MYB TF, MtMYB61, identified as an interactor of MtMYB3, can relieve the inhibitory effect of MtMYB3 on MtCBF4 transcription. This study, therefore, supports a model describing how MtCBF4 is regulated by antagonistic MtMYB3/MtMYB61 TFs, leading to the up-regulation of downstream targets such as MtCAS15 acting in cold acclimation in M. truncatula.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047070PMC
http://dx.doi.org/10.1104/pp.16.00051DOI Listing
October 2016

Genome-Wide Association Study Provides Insight into the Genetic Control of Plant Height in Rapeseed (Brassica napus L.).

Front Plant Sci 2016 27;7:1102. Epub 2016 Jul 27.

National Key Laboratory of Crop Genetic Improvement, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University Wuhan, China.

Plant height is a key morphological trait of rapeseed. In this study, we measured plant height of a rapeseed population across six environments. This population contains 476 inbred lines representing the major Chinese rapeseed genepool and 44 lines from other countries. The 60K Brassica Infinium® SNP array was utilized to genotype the association panel. A genome-wide association study (GWAS) was performed via three methods, including a robust, novel, nonparametric Anderson-Darling (A-D) test. Consequently, 68 loci were identified as significantly associated with plant height (P < 5.22 × 10(-5)), and more than 70% of the loci (48) overlapped the confidence intervals of reported QTLs from nine mapping populations. Moreover, 24 GWAS loci were detected with selective sweep signals, which reflected the signatures of historical semi-dwarf breeding. In the linkage disequilibrium (LD) decay range up-and downstream of 65 loci (r (2) > 0.1), we found plausible candidates orthologous to the documented Arabidopsis genes involved in height regulation. One significant association found by GWAS colocalized with the established height locus BnRGA in rapeseed. Our results provide insights into the genetic basis of plant height in rapeseed and may facilitate marker-based breeding.
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http://dx.doi.org/10.3389/fpls.2016.01102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961929PMC
August 2016

Genome-wide association study reveals the genetic architecture of flowering time in rapeseed (Brassica napus L.).

DNA Res 2016 Feb 10;23(1):43-52. Epub 2015 Dec 10.

National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement, Huazhong Agricultural University, Wuhan 430070, China.

Flowering time adaptation is a major breeding goal in the allopolyploid species Brassica napus. To investigate the genetic architecture of flowering time, a genome-wide association study (GWAS) of flowering time was conducted with a diversity panel comprising 523 B. napus cultivars and inbred lines grown in eight different environments. Genotyping was performed with a Brassica 60K Illumina Infinium SNP array. A total of 41 single-nucleotide polymorphisms (SNPs) distributed on 14 chromosomes were found to be associated with flowering time, and 12 SNPs located in the confidence intervals of quantitative trait loci (QTL) identified in previous researches based on linkage analyses. Twenty-five candidate genes were orthologous to Arabidopsis thaliana flowering genes. To further our understanding of the genetic factors influencing flowering time in different environments, GWAS was performed on two derived traits, environment sensitivity and temperature sensitivity. The most significant SNPs were found near Bn-scaff_16362_1-p380982, just 13 kb away from BnaC09g41990D, which is orthologous to A. thaliana CONSTANS (CO), an important gene in the photoperiod flowering pathway. These results provide new insights into the genetic control of flowering time in B. napus and indicate that GWAS is an effective method by which to reveal natural variations of complex traits in B. napus.
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http://dx.doi.org/10.1093/dnares/dsv035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4755526PMC
February 2016

Interaction between PVY HC-Pro and the NtCF1β-subunit reduces the amount of chloroplast ATP synthase in virus-infected tobacco.

Sci Rep 2015 Oct 26;5:15605. Epub 2015 Oct 26.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.

The photosynthetic rate of virus-infected plants is always reduced. However, the molecular mechanism underlying this phenomenon remains unclear. The helper component-proteinase (HC-Pro) of Potato virus Y (PVY) was found in the chloroplasts of PVY-infected tobacco, indicating some new function of HC-Pro in the chloroplasts. We generated HC-Pro transgenic plants with a transit peptide to target the protein to chloroplast. The HC-Pro transgenic tobacco showed a decreased photosynthetic rate by 25% at the light intensity of 600 μmol m(-2) s(-1). Using a yeast two-hybrid screening assay to search for chloroplast proteins interacting with HC-Pro, we identified that PVY HC-Pro can interact with the chloroplast ATP synthase NtCF1β-subunit. This interaction was confirmed by GST pull-down and co-immunoprecipitation assays. HC-Pro didn't interfere with the activity of assembled ATP synthase in vitro. The HC-Pro/NtCF1β-subunit interaction might affect the assembly of ATP synthase complex. Quantitative western blot and immunogold labeling of the ATP synthase indicated that the amount of ATP synthase complex was decreased in both the HC-Pro transgenic and the PVY-infected tobacco. These results demonstrate that HC-Pro plays an important role in reducing the photosynthetic rate of PVY-infected plants, which is a completely new role of HC-Pro besides its multiple known functions.
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http://dx.doi.org/10.1038/srep15605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620480PMC
October 2015

Potato virus Y HC-Pro Reduces the ATPase Activity of NtMinD, Which Results in Enlarged Chloroplasts in HC-Pro Transgenic Tobacco.

PLoS One 2015 26;10(8):e0136210. Epub 2015 Aug 26.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, P.R. China.

Potato virus Y (PVY) is an important plant virus and causes great losses every year. Viral infection often leads to abnormal chloroplasts. The first step of chloroplast division is the formation of FtsZ ring (Z-ring), and the placement of Z-ring is coordinated by the Min system in both bacteria and plants. In our lab, the helper-component proteinase (HC-Pro) of PVY was previously found to interact with the chloroplast division protein NtMinD through a yeast two-hybrid screening assay and a bimolecular fluorescence complementation (BiFC) assay in vivo. Here, we further investigated the biological significance of the NtMinD/HC-Pro interaction. We purified the NtMinD and HC-Pro proteins using a prokaryotic protein purification system and tested the effect of HC-Pro on the ATPase activity of NtMinD in vitro. We found that the ATPase activity of NtMinD was reduced in the presence of HC-Pro. In addition, another important chloroplast division related protein, NtMinE, was cloned from the cDNA of Nicotiana tabacum. And the NtMinD/NtMinE interaction site was mapped to the C-terminus of NtMinD, which overlaps the NtMinD/HC-Pro interaction site. Yeast three-hybrid assay demonstrated that HC-Pro competes with NtMinE for binding to NtMinD. HC-Pro was previously reported to accumulate in the chloroplasts of PVY-infected tobacco and we confirmed this result in our present work. The NtMinD/NtMinE interaction is very important in the regulation of chloroplast division. To demonstrate the influence of HC-Pro on chloroplast division, we generated HC-Pro transgenic tobacco with a transit peptide to retarget HC-Pro to the chloroplasts. The HC-Pro transgenic plants showed enlarged chloroplasts. Our present study demonstrated that the interaction between HC-Pro and NtMinD interfered with the function of NtMinD in chloroplast division, which results in enlarged chloroplasts in HC-Pro transgenic tobacco. The HC-Pro/NtMinD interaction may cause the formation of abnormal chloroplasts in PVY-infected plants.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0136210PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4550256PMC
June 2016

Formation of intercalation compound of kaolinite-glycine via displacing guest water by glycine.

J Colloid Interface Sci 2014 Oct 22;432:278-84. Epub 2014 Jun 22.

State Key Laboratory of Silicon Materials, Department of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address:

The kaolinite-glycine intercalation compound was successfully formed by displacing intercalated guest water molecules in kaolinite hydrate as a precursor. The microstructure of the compound was characterized by X-ray diffraction, Fourier Transform Infrared Spectroscopy and Scanning Electron Microscope. Results show that glycine can only be intercalated into hydrated kaolinite to form glycine-kaolinite by utilizing water molecules as a transition phase. The intercalated glycine molecules were squeezed partially into the ditrigonal holes in the silicate layer, resulting in the interlayer distance of kaolinite reaching 1.03nm. The proper intercalation temperature range was between 20°C and 80°C. An intercalation time of 24h or above was necessary to ensure the complete formation of kaolinite-glycine. The highest intercalation degree of about 84% appeared when the system was reacted at the temperature of 80°C for 48h. There were two activation energies for the intercalation of glycine into kaolinite, one being 21kJ/mol within the temperature range of 20-65°C and the other 5.8kJ/mol between 65°C and 80°C. The intercalation degree (N) and intercalation velocity (v) of as a function of intercalation time (t) can be empirically expressed as N=-79.35e(-)(t)(/14.8)+80.1 and v=5.37e(-)(t)(/14.8), respectively.
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http://dx.doi.org/10.1016/j.jcis.2014.06.016DOI Listing
October 2014
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