Publications by authors named "Zhenhua Zhu"

109 Publications

Changes of serum IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 in COVID-19 patients.

Int J Clin Pract 2021 Jun 9:e14462. Epub 2021 Jun 9.

The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, Postal Code: 410007, China.

Background And Purpose: Studies have shown that some cytokines in COVID-19 patients were elevated. This study aims to assess whether IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 serve as potential diagnostic biomarkers of COVID-19.

Methods: The above serum cytokines in COVID-19 patients and non COVID-19 patients were detected by ELISA, SARS-CoV-2 IgM and IgG were detected by chemiluminescence method. Independent-samples Mann-Whitney U-test was utilized to compare cytokines levels in different groups and course, Levene T-test, T'-test were utilized to compare they in different genders, and Spearman Correlation test was utilized to analyze the correlation between the cytokines levels with ages, SARS-CoV-2 IgG and IgM.

Results: Serum levels of IL-10, IL-1β, MCP-1, TNF-α and IL-4 in COVID-19 patients were significantly higher than those in non-COVID-19 patients, while IL-6 were only significantly higher than in healthy people, IP-10 were significantly lower than in other diseases patients. AUCs of COVID-19 diagnosed by IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 were 0.735, 0.775, 0.595, 0.821, 0.848, 0.387 and 0.682, respectively. In COVID-19 patients' serum, the levels of IL-10 and MCP-1 of male had noticeably higher than those of female, and all cytokines were significantly positively correlated with age, IL-1β and IL-4 were significantly negatively correlated with SARS-CoV-2 IgM, while IL-10, IL-1β, IL-6, TNF-α and IP-10 were significantly negatively correlated with SARS-CoV-2 IgG. IL-10 on 43-56 days were significantly lower than at 29-42 days, TNF-α at 15-42 days were significantly higher than at 0-14 days, IP-10 at 0-14 days were the highest, IL-4 at 29-42 days were significant higher than on 0-14 days.

Conclusions: The detection of IL-10, IL-1 β, IL-6, MCP-1, TNF-α and IL-4 would assist the clinical study of COVID-19, and IP-10 may be the cytokine `of early elevation in COVID-19 patients.
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http://dx.doi.org/10.1111/ijcp.14462DOI Listing
June 2021

Clinical and genetic analysis of classical Ehlers-Danlos syndrome patient caused by synonymous mutation in COL5A2.

Mol Genet Genomic Med 2021 May 8;9(5):e1632. Epub 2021 Apr 8.

Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, PR China.

Background: Classical Ehlers-Danlos syndrome (cEDS) is a heterogeneous connective tissue disorder that mainly results from the germline mutation of COL5A1 and COL5A2. The majority of the COL5A2 mutations reported to date represent structural mutations, including missense or in-frame exon-skipping splice mutations. The only reported synonymous mutation was expected to affect on splicing of exon 29 by prediction programs which should be further confirmed.

Methods: Whole exome sequencing was performed to identify the genetic variants of a Chinese boy who was characterized by skin hyperextensibility, abnormal scarring, hypermobile joints and scoliosis. Sanger sequencing was used to validate the variants in his parents. Reverse transcription polymerase chain reaction (RT-PCR) was performed to analyze the functional effects of the variant.

Results: A de novo heterozygous synonymous variant (NM_000393.5:c.1977 G>A) of COL5A2 gene was identified in the patient. The results of RT-PCR revealed that the synonymous variant led to skipping of exon 29 in the RNA transcript.

Conclusions: Our study supplies further supporting evidence that the synonymous COL5A2 mutation c.1977 G>A can cause skipping of exon 29 in the RNA transcript, thus resulting in the production of mutant α2(V)-chains and clinical phenotype of cEDS. This result highlights the need to include splicing-altering synonymous mutations into the screening for cEDS.
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http://dx.doi.org/10.1002/mgg3.1632DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172199PMC
May 2021

Open reduction and internal fixation for displaced Salter-Harris type II fractures of the distal tibia: a retrospective study of sixty-five cases in children.

J Orthop Surg Res 2021 Mar 27;16(1):224. Epub 2021 Mar 27.

Children's Hospital of Soochow University, No.92 Zhongnan Street, Suzhou Industiral Park, Suzhou, Jiangsu province, China.

Background: The treatment for displaced Salter-Harris II (S-H II) distal tibia fractures remains controversial. The purpose of this study was to review S-H II distal tibia fractures and evaluate the rate of premature physeal closure (PPC) treated by open reduction and internal fixation (ORIF).

Methods: We reviewed the charts and radiographs of S-H II fractures of the distal tibia with displacement > 3 mm between 2012 and 2019 treated by ORIF. Patients were followed up for a minimum of 6 months. CT scans of injured side or contralateral ankle radiograph were obtained if there was any evidence of PPC. Any angular deformity or shortening of the involved leg was documented. Multivariable logistic regression was performed to identify risk factors for the occurrence of PPC.

Results: A total of 65 patients with a mean age of 11.8 years were included in this study. The mean initial displacement was 8.0 mm. All patients but one were treated within 7 days after injury and the mean interval was 3.7 days. Supination-external rotation injuries occurred in 50 patients, pronation-eversion external rotation in 13, and supination-plantar flexion in two. The residual gap was less than 1 mm in all patients following ORIF and all fractures healed within 4-6 weeks. Superficial skin infection developed in one patient. Ten patients complained of the cosmetic scar. The rate of PPC was 29.2% and two patients with PPC developed a varus deformity of the ankle. Patients with associated fibular fracture had 7 times greater odds of developing PPC. Age, gender, injured side, mechanism of injury, amount of initial displacement, interval from injury to surgery, or energy of injury did not significantly affect the rate of PPC.

Conclusions: ORIF was an effective choice of treatment for S-H II distal tibia fractures with displacement > 3 mm to obtain a satisfactory reduction. PPC is a common complication following ORIF. The presence of concomitant fibula fracture was associated with PPC.
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http://dx.doi.org/10.1186/s13018-021-02359-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004420PMC
March 2021

MiR-155 promotes colitis-associated intestinal fibrosis by targeting HBP1/Wnt/β-catenin signalling pathway.

J Cell Mol Med 2021 May 26;25(10):4765-4775. Epub 2021 Mar 26.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Intestinal fibrosis is the most common complication of Crohn's disease (CD) that is one major disorder of inflammatory bowel disease (IBD), but the precise mechanism remains unclear. MiR-155 has been involved in fibrotic diseases. Here, we determined the role of miR-155 in regulating intestinal fibrosis. MiR-155 levels were significantly up-regulated in CD patients with intestinal stricture CD. The overexpression of miR-155 significantly aggravated TNBS-induced CD-associated intestinal fibrosis. Mechanistically, we identified that HBP1, a negative regulator of the Wnt/β-catenin signalling pathway, is a direct target of miR-155. Moreover, in vitro and in vivo experiments suggested that the miR-155/HBP1 axis activates Wnt/β-catenin signalling pathway to induce intestinal fibrosis. Taken together, we demonstrated that miR-155 directly targets HBP1 to induce CD-associated intestinal fibrosis via Wnt/β-catenin signalling pathway.
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http://dx.doi.org/10.1111/jcmm.16445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107084PMC
May 2021

Antibiotics for fever in patients without perforation after gastric endoscopic submucosal dissection and endoscopic submucosal excavation may be unnecessary: a propensity score-matching analysis.

BMC Gastroenterol 2021 Feb 12;21(1):64. Epub 2021 Feb 12.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, 17 Yongwaizheng, Street, Nanchang, 330006, Jiangxi Province, China.

Background: Endoscopic submucosal dissection (ESD) and endoscopic submucosal excavation (ESE) have been widely used and have gradually become the main endoscopic treatment for gastrointestinal mucosal and submucosal lesions. Whether antibiotics are necessary for fever after gastric ESD and ESE remain unclear. The aim of this study was to analyse the value of using antibiotics in patients without perforation after ESD or ESE with fever.

Methods: In this retrospective study, patients with fever without perforation after ESD or ESE from January 2014 to January 2019 were included and divided into 2 groups: the antibiotic group and the non-antibiotic group. Fever and hospitalization time were compared between the 2 groups after propensity score matching.

Results: Overall, 253 patients meeting the inclusion criteria were enrolled in the present study, with 186 patients in the non-antibiotic group and 67 patients in the antibiotic group before matching, 55 patients in the non-antibiotic group and 55 patients in the antibiotic group after matching with all baseline characteristics balanced (p > 0.05). The duration of fever was not significantly different between the 2 groups (p = 0.12). However, the median hospitalization stay in the antibiotic group was longer than that in the non-antibiotic group (8 vs 7, p = 0.007).

Conclusions: Antibiotics may be unnecessary for fever in patients without perforation and without serious co-morbidities after gastric ESD or ESE.
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http://dx.doi.org/10.1186/s12876-021-01602-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879668PMC
February 2021

High-Dose Proton Pump Inhibitors Are Superior to Standard-Dose Proton Pump Inhibitors in High-Risk Patients With Bleeding Ulcers and High-Risk Stigmata After Endoscopic Hemostasis.

Clin Transl Gastroenterol 2021 Jan 14;12(1):e00294. Epub 2021 Jan 14.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.

Introduction: To define the best cutoff of the Glasgow-Blatchford score (GBS) for identifying high- and low-risk rebleeding patients with bleeding ulcers and high-risk stigmata after endoscopic hemostasis and compare the efficacy of high-dose and standard-dose intravenous proton pump inhibitors (HD-IVPs and SD-IVPs, respectively) in this patient population.

Methods: We retrospectively reviewed the data of 346 patients with bleeding ulcers and high-risk stigmata who underwent endoscopic hemostasis between March 2014 and September 2018 in our center and were divided into an HD-IVP group and an SD-IVP group. Propensity score-matching analysis was performed to control for selection bias and other potential confounders. Recurrent bleeding rates were calculated according to the GBS.

Results: Overall, 346 patients meeting the inclusion criteria were enrolled, with 89 patients in the SD-IVP group and 89 patients in the HD-IVP group after matching with all baseline characteristics balanced (P > 0.05). GBS = 8 was the best cutoff for identifying high-risk rebleeding patients (GBS ≥ 8) with a significant difference (P = 0.015) in recurrence rate between the SD-IVP (17/61, 27.9%) and HD-IVP (7/65, 10.8%) groups and low-risk rebleeding patients (GBS < 8) with no difference (P = 1) in recurrence rate between the SD-IVP (2/28, 7.1%) and HD-IVP (2/24, 8.3%) groups.

Discussion: The best cutoff for identifying high-risk and low-risk rebleeding patients with bleeding ulcers and high-risk stigmata after endoscopic hemostasis was GBS = 8. Although HD-IVP is more effective than SD-IVP in high-risk patients, they are equally effective in low-risk patients.
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http://dx.doi.org/10.14309/ctg.0000000000000294DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810506PMC
January 2021

Dendrobium officinale flos increases neurotrophic factor expression in the hippocampus of chronic unpredictable mild stress-exposed mice and in astrocyte primary culture and potentiates NGF-induced neuronal differentiation in PC12 cells.

Phytother Res 2021 Jan 12. Epub 2021 Jan 12.

National and local union project research center of Chinese Medicinal Resources Industrialization and Innovative drug from TCM Formulae, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing, China.

Dendrobium officinale flos (DOF) is the flower of Dendrobium officinale Kimura et Migo, which is usually regarded as a by-product of Dendrobii Offcinalis Caulis. Based on its use as an alternative medicine, we evaluated the antidepressant-like effect of DOF extracts on chronic, unpredictable, mild stress-induced, depression-like behaviour in mice and tested the effects of DOF on the regulation of neurotrophic factors in mouse astrocyte primary cultures and PC12 cell lines. Oral treatment with DOF ethanol extract (DOF-E) could alleviate depression-like behaviours in stress-exposed mice, as evidenced by increased sucrose consumption and decreased immobile time in a forced swim test. In the hippocampus, DOF extracts increased the expression of NGF and BDNF, both at the transcriptional and protein levels. In astrocytes, DOF-E increased the expression of NGF and BDNF via a cAMP-dependent mechanism and regulated plasminogen and matrix metallopeptidase 9 (MMP-9), which are related to the metabolic regulation of neurotrophic factors. In PC12 cells, DOF-E induced the expression of neurofilaments and potentiated the induction of neurite outgrowth upon treatment with a low dose of NGF. Based on these findings, DOF might be used as a supplement for antidepressant therapy in patients with depression.
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http://dx.doi.org/10.1002/ptr.7013DOI Listing
January 2021

Enhanced flotation efficiency of metal from waste printed circuit boards modified by alkaline immersion.

Waste Manag 2021 Feb 22;120:795-804. Epub 2020 Nov 22.

Kanfort (Jiangmen) Environmental Technology Co., Ltd, Jiangmen 529060, China.

Efficient recycling of waste printed circuit boards by flotation has become a research focus. In this study, waste printed circuit boards were treated by alkaline immersion to enhance the flotation efficiency. Firstly, the SEM-EDS analysis of the crushed products shown that metal and nonmetal were completely liberated in the -0.25 mm fraction. When the printed circuit boards were modified by alkaline immersion, the recovery of metal increased from 64.34% to 72.35%. Further, the mixture of metal and nonmetal at the edge of nonmetal was discovered by EPMA. This was the cause of metal loss during the flotation process. Secondly, by adjusting the alkaline immersion time and pH value, a good flotation effect was achieved at 40 min alkaline immersion time and the pH = 11. Meanwhile, the XPS analysis of nonmetal found that the intensity of the OH peak was significantly enhanced, while the intensity of the O peak was evidently decreased. The change of the resin molecular structure indicated that the O linked to the benzene ring was broken under the action of alkaline immersion, resulting a free bond was generated on the benzene ring. This made the free OH adsorb to the free bond. This conduct promoted the dispersion of nonmetal in the slurry due to the increased nonmetal surface energy and metal hydrophilicity. Thus, this study provides a new route to improve the flotation efficiency of waste printed circuit boards.
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http://dx.doi.org/10.1016/j.wasman.2020.11.002DOI Listing
February 2021

Risk Factors for Rebleeding after Emergency Endoscopic Treatment of Dieulafoy Lesion.

Can J Gastroenterol Hepatol 2020 24;2020:2385214. Epub 2020 Aug 24.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China.

: Dieulafoy lesion is a rare, but life-threatening, cause of gastrointestinal hemorrhage, and endoscopic therapy is the preferred first-line treatment. The present study aims to analyze the risk factors for rebleeding after endoscopic hemostasis of gastroduodenal Dieulafoy lesion. . A retrospective review of patients with Dieulafoy lesion who developed acute gastrointestinal bleeding and were treated primarily with endoscopic therapy from September 2014 to April 2019 was conducted. . A total of 133 patients with Dieulafoy lesion were included in the present study. The mean age of these patients was 56.05 ± 16.58 years, and 115 patients were male. Among these 133 patients, 26 patients developed rebleeding within 30 days of endoscopic therapy. The 30-day rebleeding rate for pure injection therapy (epinephrine, cyanoacrylate, or lauromacrogol injection alone), nonpure injection therapy (argon plasma coagulation, band ligation, and hemoclip application alone), and combination therapy (combination of any >2 methods) was 45.2%, 12.8%, and 11%, respectively. In the univariable analysis, endoscopic treatment, prothrombin time, gender, Rockall score, and leukocyte count were the risk factors for rebleeding. In the multivariable analysis, pure injection endoscopic treatment, white blood cells (>10 × 10/L), and prothrombin time >12 seconds were the independent risk factors for rebleeding. . Patients who undergo pure injection endoscopic treatment and have a high leukocyte count (>10 × 10/L) or elevated prothrombin time (>12 seconds) have an increased risk of rebleeding within 30 days after endoscopic treatment for gastroduodenal Dieulafoy lesion. Combined endoscopic treatment is the most effective therapy to prevent rebleeding in gastroduodenal Dieulafoy lesion.
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http://dx.doi.org/10.1155/2020/2385214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468603PMC
August 2020

Trans-omic profiling between clinical phenoms and lipidomes among patients with different subtypes of lung cancer.

Clin Transl Med 2020 Aug;10(4):e151

Institute of Clinical Science, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Lung cancer has high mortality, often accompanied with systemic metabolic disorders. The present study aimed at defining values of trans-nodules cross-clinical phenomic and lipidomic network layers in patients with adenocarcinoma (ADC), squamous cell carcinomas, or small cell lung cancer (SCLC). We measured plasma lipidomic profiles of lung cancer patients and found that altered lipid panels and concentrations varied among lung cancer subtypes, genders, ages, stages, metastatic status, nutritional status, and clinical phenome severity. It was shown that phosphatidylethanolamine elements (36:2, 18:0/18:2, and 18:1/18:1) were SCLC specific, whereas lysophosphatidylcholine (20:1 and 22:0 sn-position-1) and phosphatidylcholine (19:0/19:0 and 19:0/21:2) were ADC specific. There were statistically more lipids declined in male, <60 ages, late stage, metastasis, or body mass index < 22 . Clinical trans-omics analyses demonstrated that one phenome in lung cancer subtypes might be generated from multiple metabolic pathways and metabolites, whereas a metabolic pathway and metabolite could contribute to different phenomes among subtypes, although those needed to be furthermore confirmed by bigger studies including larger population of patients in multicenters. Thus, our data suggested that trans-omic profiles between clinical phenomes and lipidomes might have the value to uncover the heterogeneity of lipid metabolism among lung cancer subtypes and to screen out phenome-based lipid panels as subtype-specific biomarkers.
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http://dx.doi.org/10.1002/ctm2.151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438979PMC
August 2020

Counter anions influence the relaxation dynamics of phenoxy-bridged Dy single molecule magnets.

Dalton Trans 2020 Sep;49(35):12372-12379

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.

The syntheses, structures and magnetic properties of a series of dimeric dysprosium(iii) complexes [Dy2L2(CH3OH)(H2O)]·2X·solvent {X = Cl (1), NO3 (2), ClO4 (3)} and [Dy2L2(CH3OH)2]·2X·solvent {X = CF3SO3 (4)}, formed from the 1 : 1 reactions of the H2L ligand with the corresponding dysprosium salts, are reported. Structural and magnetic studies reveal that counter anions on the periphery play a significant role in determining the dynamic magnetic relaxation process of these complexes. The coordination geometries of the Dy(1)(iii) centers are eight-coordinate triangular dodecahedra in 1-4. All compounds exhibit single-molecule magnet (SMM) behavior under a zero dc field and optimal applied dc field except 3, which displays only slow relaxation of magnetization. A comparison of the magnetic properties and structural parameters of the four compounds shows that the short Dy-Ophen distances and the large Ophen-Dy-Ophen angles create an axial ligand field in which dysprosium(iii) complexes exhibit magnetic anisotropy and SMM properties.
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http://dx.doi.org/10.1039/d0dt02451aDOI Listing
September 2020

Inhibition of protein kinase D by CID755673 promotes maintenance of the pluripotency of embryonic stem cells.

Development 2020 08 24;147(16). Epub 2020 Aug 24.

Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China

The identification of novel mechanisms to maintain embryonic stem cell (ESC) pluripotency is of crucial importance, because the currently used culture conditions are not suitable for ESCs from all species. In this study, we show that the protein kinase D (PKD) inhibitor CID755673 (CID) is able to maintain the undifferentiated state of mouse ESCs in combination with the mitogen-activated protein kinase kinase (MEK) inhibitor. The expression levels of PKD members, including PKD1, PKD2 and PKD3, were low in mouse ESCs but significantly increased under differentiation conditions. Therefore, depletion of three PKD genes was able to phenocopy PKD inhibition. Mechanistically, PKD inhibition activated PI3K/AKT signaling by increasing the level of AKT phosphorylation, and the addition of a PI3K/AKT signaling pathway inhibitor partially reduced the cellular response to PKD inhibition. Importantly, the self-renewal-promoting effect of CID was maintained in human ESCs. Simultaneous knockdown of the three human PKD isoforms enabled short-term self-renewal in human ESCs, whereas PI3K/AKT signaling inhibition eliminated this self-renewal ability downstream of the PKD inhibitor. These findings expand our understanding of the gene regulatory network of ESC pluripotency.
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http://dx.doi.org/10.1242/dev.185264DOI Listing
August 2020

Knockdown of inhibits growth of tumors in soft tissue sarcoma and .

J Cancer 2020 28;11(17):5088-5098. Epub 2020 Jun 28.

Department of Orthopaedic Trauma, The First Hospital of Jilin University, Xinmin Street 71#, Changchun City, Jilin Province, China.

Kif20 (Kinesin Family Member 20A), plays a role in cell mitosis, cell migration and intracellular transport. Numerous studies have demonstrated that Kif20a is abnormally highly expressed in a variety of tumors and shows poor prognosis. Soft tissue sarcoma (STS) represents a group of malignant tumors with poor prognosis. The role of Kif20 in STSs has not been systematically studied. In the present study, bioinformatics analysis, and experiments were conducted to investigate the function of Kif20 in STSs. In bioinformatics analysis higher expression indicated a poor prognosis. Functional enrichment analysis indicated that may be related to cell cycle, p53 and other signaling pathways. experiments showed that after the down-regulation of , cell proliferation, migration and invasion were decreased, while apoptosis was increased. experiments revealed that affected the proliferation of tumors in tumor-bearing mice. In summary, our findings revealed that performs an important role in STS, indicating that it is a potential prognostic biomarker and potentially representing a therapeutic target for the disease.
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http://dx.doi.org/10.7150/jca.44777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378921PMC
June 2020

Clinical and radiological outcomes of combined acetabuloplasty with acetabular redirectional osteotomy and femoral shortening for children older than 9 years of age with developmental dysplasia of the hip: a retrospective case series.

J Pediatr Orthop B 2020 Sep;29(5):417-423

Department of Pediatric Orthopaedics, Beijing Jishuitan Hospital, Xicheng district, Beijing, China.

Developmental dysplasia of the hip (DDH) is a challenging pediatric condition. This study aimed to investigate the feasibility and the efficacy of Dega osteotomy in combination with pelvic triple osteotomy or periacetabular osteotomy and femoral shortening with derotational osteotomy for the treatment of severe acetabular deformity secondary to DDH in children older than 9 years of age. The clinical data of 22 children treated at our institution were retrospectively collected. Pre- and postoperative hip radiographs were evaluated. Center-edge (CE) angle was measured, and Severin classification and McKay criteria were used to evaluate the final outcome. Twenty-two patients (including 21 female patients) were treated during the study period. The mean age was 10.9 years (range 9.1-14.8). All patients had preoperative Tonnis grades III and IV dislocated hips. Mean follow-up time was 25.7 months (range 14-48). All but three hips underwent open reduction. All had concomitant proximal femoral shortening and derotation osteotomy. At last follow-up visit, all hips remained located and no cases of avascular necrosis were recorded. All patients had Severin grade I-II hips at last follow-up. According to McKay criteria, clinical outcome was excellent in three patients, good in seven, and fair in 12 patients. Acetabuloplasty in combination with periacetabular osteotomy and femoral shortening with derotational osteotomy is a valid and effective treatment for children older than 9 years of age with severe acetabular deformity secondary to DDH. Although the early clinical outcome of the reported procedure is favorable, further studies are necessary to evaluate the long-term effects of the reported technique.
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http://dx.doi.org/10.1097/BPB.0000000000000774DOI Listing
September 2020

A novel serum miRNA-pair classifier for diagnosis of sarcoma.

PLoS One 2020 16;15(7):e0236097. Epub 2020 Jul 16.

Department of Orthopaedic Trauma, The First Hospital of Jilin University, Changchun, Jilin Province, China.

Soft tissue sarcomas (STS) is a set of rare malignant tumor originated from mesoderm. For the prognosis of sarcoma, early diagnosis is important, however, currently no mature and non-invasive method for diagnosis exists. MicroRNAs (miRNAs) are a class of noncoding RNAs and their expression varies greatly, especially during tumor activity. The purpose of this study was to construct a predictive model for the diagnosis of sarcomas based on the relative expression level of miRNA in serum. miRNA array expression data of 677 samples including 402 malignant sarcoma samples and 275 healthy samples was used to construct the prediction model. Based on 6 gene pairs, random generalized linear model (RGLM) was constructed, with an accuracy of 100% in the internal test dataset and of 74.3% in the merged external dataset in prediction whether a serum sample was obtained from a sarcoma patient, with a specificity of 100% in the internal test dataset and 90.5% in the external dataset. In conclusion, our serum miRNA-pair classifier has the potential to be used for the screening of sarcoma with high accuracy and specificity.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0236097PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365454PMC
September 2020

Novel Predictive Nomogram for Identifying Difficult Guidewire Insertion in Patients With Malignant Colorectal Obstruction and Sphincterotome-Assisted Guidewire Insertion for Improving the Success Rate of Self-Expandable Metal Stent Insertion.

Front Oncol 2020 13;10:637. Epub 2020 May 13.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

This study aimed to identify risk factors related to guidewire insertion (GWI) failure and construct a novel predictive nomogram. In addition, sphincterotome-assisted guidewire insertion (SAGWI) in difficult cases was evaluated for efficacy and safety. We reviewed the data of 509 patients with malignant colorectal obstruction who underwent endoscopic self-expandable metal stent (SEMS) insertion from 2007 to 2018 in our center, retrospectively. We identify risk factors associated with GWI failure by multivariate logistic regression analysis and construct a novel predictive nomogram. Improvements in the GWI and technical and clinical success rates were assessed for the SAGWI technique. A total of 509 patients with malignant colorectal obstruction were included. Increases of 6.9% and 7.0% were found in the GWI success rate by intention-to-treat (ITT; < 0.001) and per-protocol (PP; < 0.001) analyses after SAGWI, respectively. Increases of 6.5% and 6.6% in the technical success rate were found by ITT ( < 0.001) and PP ( < 0.001) analyses after SAGWI, respectively. Increases of 5.8% and 6.0% in the clinical success rate were found by ITT ( < 0.001) and PP ( < 0.001) analyses after SAGWI, respectively. Regarding the GWI failure-related factors, a sharply angulated stricture was an independent risk factor, and an experienced colonoscopist was an independent protective factor. A novel effective predictive nomogram was constructed. The novel predictive nomogram can be conveniently used to identify difficult cases. A sharply angulated stricture and an experienced colonoscopist are independent factors related to GWI failure. The SAGWI technique is an effective and safe method for addressing technically difficult cases.
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http://dx.doi.org/10.3389/fonc.2020.00637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7237730PMC
May 2020

Focal fibrocartilaginous dysplasia of the distal femur.

J Pediatr Orthop B 2020 Sep;29(5):466-471

Department of Pediatric Orthopaedics, Beijing Jishuitan Hospital, Beijing, China.

Focal fibrocartilaginous dysplasia (FFCD) of the distal femur is a rare disorder that results in a varus or valgus of knee. Due to the small number of cases and unconfirmed natural history of the disease, treatment methods remain variable. This study aimed to determine a strategy to successfully manage distal femoral FFCD. Nine case of femoral FFCD treated in our department between 2008 and 2018, together with 22 cases from literature, were retrospectively reviewed. Tibiofemoral anatomical axis angle (TFA) was used to evaluate and follow the deformities. Treatment methods and outcome were analyzed. Five methods were used to manage the disease: osteotomy with tether release in 14 cases with mean TFA of 29°; simple tether release in eight cases with mean TFA of 31°; guide growth without tether release in 3 case with mean TFA of 27°; guide growth with tether release in 3 case with mean TFA of 27°; and observation in three cases with mean TFA of 23°.Deformity was resolved in all 31 patients. The analysis of the 31 cases in the literature and our experience suggests that femoral FFCD can be successfully managed by simple tether release and curettage. Osteotomy can be avoided. In case of mild deformity (TFA < 25°), it is reasonable to follow-up till 2-3 years of age; if no progress occurs, spontaneous resolution can be expected.
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http://dx.doi.org/10.1097/BPB.0000000000000742DOI Listing
September 2020

Comparative Analysis of the Chemical Consistency Between the Traditional and Mixed Decoction of Maimendong Decoction by Ultra-Performance Liquid Chromatography Coupled to Quadrupole with Time-of-Flight Mass Spectrometry (UPLC-QTOF-MS)-Based Chemical Profiling Approach.

J Chromatogr Sci 2020 Jun;58(6):549-561

Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China.

Take Maimendong Decoction (MMDD), one of the Chinese classic herbal formulas, as an object to evaluate the chemical consistency between traditional decoction and mixed decoction. The ultra-performance liquid chromatography coupled to quadrupole with time-of-flight mass spectrometry-based chemical profiling approach has been utilized. A total of 48 major peaks are detected from these two decoctions under the present chromatographic and mass spectrometry conditions. The results of negative ion mode show nine significant inconsistencies. Liquiritin, ginsenoside Ro and ginsenoside Rg5/Rk1 are detected with higher intensity in traditional preparation sample than the mixed decoction, while licoisoflavone A is higher in mixed decoction samples than the traditional one. The mechanisms involved in the chemical changes were assumed to be anti-inflammation, anti-oxidative stress and so on, suggesting these two different preparation approaches of MMDD may lead to a possibility of discrepant clinical outcomes.
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http://dx.doi.org/10.1093/chromsci/bmz104DOI Listing
June 2020

Linked Color Imaging Can Improve Detection Rate of Early Gastric Cancer in a High-Risk Population: A Multi-Center Randomized Controlled Clinical Trial.

Dig Dis Sci 2021 Apr 4;66(4):1212-1219. Epub 2020 May 4.

Xi'an Central Hospital, Xi'an, China.

Background: Early diagnosis of gastric cancer is difficult in China due to the lack of a valid method for endoscopic screening. Early gastric cancer, especially flat gastric cancer, lacks specific endoscopic features. Many cases appear to be similar to ordinary gastritis cases under normal white light endoscopy, which can lead to misdiagnosis.

Aims: In order to find a new method to improve detection rate of early gastric cancer in China, we designed a trial to validate linked color imaging (LCI) for screening of early gastric cancer in a high-risk population, as compared to white light imaging (WLI).

Method: Subjects were randomly allocated to either the LCI + WLI or WLI group and then subjected to gastroscopy and all endoscopies were made after special preparation. All endoscopists had knowledge of this experiment. The main indicator was the rate of detection of gastric neoplastic lesions. The difference in the detection rate between the two groups is reported.

Results: The detection rate was 4.31% in the WLI group and 8.01% in the LCI + WLI group. This is a difference of 3.70% with a P value < 0.001 and an OR (95% CI) of 1.934 (1.362, 2.746). The lower limit of the 95% CI was greater than 0, and the superiority margin was 1%.

Conclusion: The detection rate of gastric neoplastic lesions was higher in the LCI + WLI group than in the WLI group, LCI might be an effective method for screening early gastric cancer.
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http://dx.doi.org/10.1007/s10620-020-06289-0DOI Listing
April 2021

Inter-Kramers Transitions and Spin-Phonon Couplings in a Lanthanide-Based Single-Molecule Magnet.

Inorg Chem 2020 Apr 20;59(7):5218-5230. Epub 2020 Mar 20.

Department of Chemistry, University of Tennessee, Knoxville, Tennessee 37996, United States.

Spin-phonon coupling plays a critical role in magnetic relaxation in single-molecule magnets (SMMs) and molecular qubits. Yet, few studies of its nature have been conducted. Phonons here refer to both intermolecular and intramolecular vibrations. In the current work, we show spin-phonon couplings between IR-active phonons in a lanthanide molecular complex and Kramers doublets (from the crystal field). For the SMM Er[N(SiMe)] (, Me = methyl), the couplings are observed in the far-IR magnetospectroscopy (FIRMS) of crystals with coupling constants ≈ 2-3 cm. In particular, one of the magnetic excitations couples to at least two phonon excitations. The FIRMS reveals at least three magnetic excitations (within the ground state/manifold; hereafter, manifold) at 0 T at 104, ∼180, and 245 cm, corresponding to transitions from the ground state, = ±15/2, to the first three excited states, = ±13/2, ±11/2, and ±9/2, respectively. The transition between the ground and first excited Kramers doublet in is also observed in inelastic neutron scattering (INS) spectroscopy, moving to a higher energy with an increasing magnetic field. INS also gives complete phonon spectra of . Periodic DFT computations provide the energies of all phonon excitations, which compare well with the spectra from INS, supporting the assignment of the inter-Kramers doublet (magnetic) transitions in the spectra. The current studies unveil and measure the spin-phonon couplings in a typical lanthanide complex and throw light on the origin of the spin-phonon entanglement.
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http://dx.doi.org/10.1021/acs.inorgchem.0c00523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935416PMC
April 2020

TRIM59 Protects Mice From Sepsis by Regulating Inflammation and Phagocytosis in Macrophages.

Front Immunol 2020 18;11:263. Epub 2020 Feb 18.

Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, China.

Sepsis is associated with bacterial invasion and inflammation and has a high mortality rate. Previous studies have demonstrated that tripartite motif 59 (TRIM59) was involved in NF-κB signaling and could promote phagocytosis of macrophages, but the role of TRIM59 in sepsis is still unknown. In our study, we found that TRIM59 was downregulated in lipopolysaccharide (LPS)-stimulated bone marrow-derived macrophages (BMDMs). In the cecal ligation and puncture (CLP) sepsis mice model, the mortality of (-cKO) mice was higher, the immune cell infiltration and damage of liver and lung were more severe, and bacteria burden was increased. We also found that TRIM59 altered the production of pro-inflammation cytokines, as well as macrophage phagocytosis ability. Further analysis indicated that NF-κB signal pathway and Fcγ receptors might be involved in these regulations. Our study demonstrated for the first time that TRIM59 protects mice from sepsis by regulating inflammation and phagocytosis in macrophages.
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http://dx.doi.org/10.3389/fimmu.2020.00263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041419PMC
March 2021

Integrative Clustering Reveals a Novel Subtype of Soft Tissue Sarcoma With Poor Prognosis.

Front Genet 2020 17;11:69. Epub 2020 Feb 17.

Department of Orthopaedic Trauma, The First Hospital of Jilin University, Changchun, China.

Background: Soft tissue sarcomas (STSs) are heterogeneous at the clinical and molecular level and need to be further sub-clustered for treatment and prognosis.

Materials And Methods: STSs were sub-clustered based on RNAseq and miRNAseq data extracted from The Cancer Genome Atlas (TCGA) through the combined process of similarity network fusion (SNF) and consensus clustering (CC). The expression and clinical characteristics of each sub-cluster were analyzed. The genes differentially expressed (lncRNAs, miRNAs, and mRNAs) between the poor prognosis and good prognosis clusters were used to construct a competing endogenous RNA (ceRNA) network. Functional enrichment analysis was conducted and a hub network was extracted from the constructed ceRNA network.

Results: A total of 247 STSs were classified into three optimal sub-clusters, and patients in cluster 2 (C2) had a significantly lower rate of survival. A ceRNA network with 91 nodes and 167 edges was constructed according to the hypothesis of ceRNA. Functional enrichment analysis revealed that the network was mainly associated with organism development functions. Moreover, LncRNA (KCNQ1OT1)-miRNA (has-miR-29c-3p)-mRNA (JARID2, CDK8, DNMT3A, TET1)-competing endogenous gene pairs were identified as hub networks of the ceRNA network, in which each component showed survival significance.

Conclusion: Integrative clustering analysis revealed that the STSs could be clustered into three sub-clusters. The ceRNA network, especially the subnetwork LncRNA (KCNQ1OT1)-miRNA (has-miR-29c-3p)-mRNA (JARID2, CDK8, DNMT3A, TET1) was a promising therapeutic target for the STS sub-cluster associated with a poor prognosis.
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http://dx.doi.org/10.3389/fgene.2020.00069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038822PMC
February 2020

β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.

Biochem Biophys Res Commun 2020 04 25;524(2):280-287. Epub 2020 Jan 25.

Center for Stem Cell and Translational Medicine, School of Life Sciences & Institute of Physical Science and Information Technology, Anhui University, Hefei, 230601, PR China. Electronic address:

Activation of the Wnt/β-catenin signaling pathway by the inhibition of glycogen synthase kinase-3 (GSK-3) will induce Tcf7l1 protein degradation to effectively promote embryonic stem cell (ESC) self-renewal. However, the exact mechanism remains unclear. Here, we found that inhibition of casein kinase 2 (Csnk2) by TBB or DMAT was sufficient to block the reduction of the Tcf7l1 protein induced by CHIR99021, a specific inhibitor of GSK-3. Similarly, downregulation of Csnk2 increased the Tcf7l1 level. In contrast, overexpression of Csnk2 significantly decreased Tcf7l1 protein stability in mouse ESCs. Notably, Csnk2α1 controls Tcf7l1 turnover to a greater degree than the other two isoforms of Csnk2, Csnk2α2 and Csnk2β, as Csnk2α1-overexpressing mouse ESCs exhibited the lowest level of Tcf7l1. Csnk2α1 interacted with and phosphorylated Tcf7l1. In addition, the association of Csnk2α1 and Tcf7l1 was enhanced by CHIR99021. Our study demonstrated, for the first time, that Csnk2 is involved in Tcf7l1 turnover mediated by the Wnt/β-catenin signaling pathway. These results expand our understanding of the function and circuit of Wnt/β-catenin signaling pathway in ESCs.
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http://dx.doi.org/10.1016/j.bbrc.2020.01.074DOI Listing
April 2020

The effect of deoxyschizandrin on chronic unpredictable mild stress-induced depression.

Biotechnol Appl Biochem 2021 Feb 7;68(1):52-59. Epub 2020 Feb 7.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, China.

The purpose of the present study was to evaluate the antidepressant effect of deoxyschizandrin (DEO) in chronic unpredictable mild stress (CUMS)-induced mice. The mice were subjected to CUMS paradigm for 8 weeks. From the sixth week, the mice were intragastrically treated with DEO once daily for continuous 3 weeks. The behavior tests including sucrose preference test (SPT), forced swimming test (FST), tail suspension test (TST), and open field test were conducted. Additionally, the expressions of TLR4, MyD88, TRAF6, p-NF-κBp65, NLRP3, cleaved caspase-1, cleaved IL-1β, GluR, and PSD95 in hippocampus were detected by western blot. The concentrations of IL-6 and TNF-α in hippocampus were determined by enzyme linked immune sorbent assay (ELISA). The dendritic spine density was observed by Golgi-Cox staining. As a result, the treatment with DEO relieved anhedonia in SPT, and reduced immobile duration in FST and TST. DEO treatment effectively attenuated the CUMS-caused alterations of TLR4, MyD88, TRAF6, p-NF-κBp65, NLRP3, cleaved caspase-1, cleaved IL-1β, GluR, and PSD95. Furthermore, DEO could reduce the hippocampal inflammatory cytokine content and increase the density of dendritic spine. In conclusion, the present work indicated that DEO exhibited antidepressant effect on CUMS-induced depressive mice, which was possible due to the TLR4/NF-κB/NLRP3 pathway and the amelioration of dendritic spine density through GluR/PSD95 cascade.
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http://dx.doi.org/10.1002/bab.1893DOI Listing
February 2021

Linear hexanuclear helical dysprosium single-molecule magnets: the effect of axial substitution on magnetic interactions and relaxation dynamics.

Dalton Trans 2019 Oct 6;48(37):14062-14068. Epub 2019 Sep 6.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China. and School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, P. R. China.

The reaction between the rigid Schiff-base ligand HL ([3,6-bis(2-hydroxy-3-methoxybenzylidene)hydrazinecarbonyl]-pyridazine) and different dysprosium(iii) salts afforded two new linear hexanuclear helical clusters, [DyL(SCN)(DMF)]·4DMF (1), and [DyL(NO)(DMF)(HO)]·8DMF (2), which possess a similar Dy core with [DyL(PhCOO)(CHOH)]·11CHOH·HO (3). Modulation of the axial ligands around Dy sites causes different coordination geometries and magnetic interactions, resulting in distinct magnetic relaxation behaviors. Compounds 1 and 2 show typical single-molecule magnet (SMM) properties with effective barriers (U) of 15 and 68 K, respectively, which could greatly profit from strong ferromagnetic interactions compared with compound 3. Presence of a hula-hoop-like geometry of the Dy ions and stronger ferromagnetic interactions results in better SMM performance of compound 2 than that of 1.
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http://dx.doi.org/10.1039/c9dt03101dDOI Listing
October 2019

Cross-Species Gene Expression Analysis Reveals Gene Modules Implicated in Human Osteosarcoma.

Front Genet 2019 7;10:697. Epub 2019 Aug 7.

Department of Orthopaedic Trauma, The First Hospital of Jilin University, Changchun, China.

Osteosarcoma (OS) is one of the malignant bone tumors occurring in both human and canine, and in both of them, it is characterized by a high rate of metastasis and poor prognosis. Cross-species analysis reveals previously neglected molecular or signaling pathways involved in the progression of diseases, and dogs are genetically comparable to humans and live in similar environments. Therefore, the aim of this study was to find out OS hub genes through a cross-species analysis. All the human and canine OS gene expression data obtained by the Affymetrix platform were collected. After quality assessment and normalization, co-expression network was performed using weighted gene co-expression network analysis (WGCNA). Species-specific modules and consensus modules were identified. Protein-protein interaction (PPI) networks analysis was performed based on consensus gene modules. Then, consensus modules were functionally annotated and correlated with clinical traits. Hub nodes were identified by a subnetwork analysis of PPI network and WGCNA module membership. Modules of interest and hub nodes were validated in an external data set. Three modules for the human network, seven modules for the canine network, and four consensus modules were identified. The consensus module 3 (C3) showed a significant correlation with the metastatic status in the training data set and a significant correlation with metastasis-free survival in the external data set. Cluster of differentiation 86 (CD86) was identified as the hub gene of C3, showing a significant correlation with metastasis-free survival. Genes in C3 play an important role in OS metastasis, whereas CD86 might be a potential molecular biomarker for OS metastasis.
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http://dx.doi.org/10.3389/fgene.2019.00697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6693360PMC
August 2019

A Surveillance, Epidemiology and End Results database analysis of the prognostic value of organ-specific metastases in patients with advanced prostatic adenocarcinoma.

Oncol Lett 2019 Aug 7;18(2):1057-1070. Epub 2019 Jun 7.

Department of Urology, The First Hospital of Jilin University, Changchun, Jilin 130012, P.R. China.

Prostate cancer (PCa) survival markedly decreases with the occurrence of distant metastasis, and treatment decisions can be influenced by metastasis site, and affect patient survival outcomes. The aim of the present study was to evaluate the potential prognostic value of metastasis to specific sites and the prognostic value of prostatectomy in patients with only bone metastasis, and to determine potential risk factors for bone metastasis in prostatic adenocarcinoma using large scale clinical data. The Surveillance, Epidemiology and End Results (SEER) database (2010-2013) was queried via the SEER*Stat (version 8.3.4) program. A total of 210,730 prostatic adenocarcinoma patients were identified from the SEER database between January 2010 and December 2013. Univariate and multivariate Cox regression analysis and Kaplan-Meier curves were used for survival comparisons with corresponding 95% confidence intervals. Patients with PCa with only liver metastatic lesions had worse overall and cancer-specific survival rates compared with those patients with only bone or lung metastasis. Multivariate Cox regression analysis revealed that age <50 years, married status, T1 and T3 tumor stage according to Tumor-Node-Metastasis (TNM) staging system from the 7th AJCC cancer staging manual, and prostatectomy were associated with better overall survival and cancer-specific survival in patients with only bone metastasis. Binary logistic regression analysis revealed that unmarried status, African descent and undifferentiated histological grade were risk factors for PCa bone metastasis. Prostatic adenocarcinoma patients with only liver metastasis had worse prognostic outcomes compared with patients with other distant organ metastases. Prostatectomy improved the 3-year survival rate in stage IV PCa patients and stage IV PCa patients with only bone metastasis. These findings were based on large-scale clinical data and can provide novel perspectives for the treatment of patients with advanced prostate adenocarcinoma.
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http://dx.doi.org/10.3892/ol.2019.10461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607368PMC
August 2019

Conservative tibiotalocalcaneal fusion for partial talar avascular necrosis in conjunction with ankle and subtalar joint osteoarthritis in Kashin-Beck disease: A case report.

Medicine (Baltimore) 2019 Jul;98(29):e16367

Department of Orthopaedics, the Second Affiliated Hospital of Soochow University, Suzhou, China.

Rationale: Kashin-Beck disease (KBD) is known for some typical characters like finger joint enlargement, shortened fingers, and dwarfism. However, Avascular necrosis (AVN) of the talus in KBD has rarely been reported in the literature. Here, we reported on a KBD patient presented with partial AVN of the talus in conjunction with ankle and subtalar arthritis.

Patient Concerns: A 50-year-old woman presented with severe pain and limited range of motion in her left ankle and subtalar joint while walking for 2 years. She had been walking with the aid of crutches for many years. Conservative treatment with rigid orthosis and activity restriction could not help reduce the pain in the left foot.

Diagnoses: Radiographs demonstrated that partial AVN was developed in the body of the talus and arthritis was viewed in the left ankle and subtalar joint. Hence, we established the diagnosis of partial talar AVN in conjunction with ankle and subtalar arthritis.

Interventions: A conservative tibiotalocalcaneal fusion attempting to preserve as much viable talar body as possible was performed using a humeral locking plate and 2 cannulated compression screws.

Outcomes: Bone union proved by CT scan and a good alignment of the left limb were achieved at 4-month follow-up postoperatively.

Lessons: Partial AVN of the talus along with ankle and subtalar arthritis in KBD patients has rarely been reported as it is not a common characteristic of KBD in clinical practice. Conservative tibiotalocalcaneal fusion could help preserving much more viable talar body, maintaining most structural integrity of the ankle joint, and achieving a stable and plantigrade foot postoperatively.
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http://dx.doi.org/10.1097/MD.0000000000016367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6709310PMC
July 2019

Comparison of the peptidome released from keratins in Saiga antelope horn and goat horn under simulated gastrointestinal digestion.

Electrophoresis 2019 10 13;40(20):2759-2766. Epub 2019 Jun 13.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing, P. R. China.

Goat horn (Caprae Hircus Cornu, GH) has been used as a substitute for Saiga antelope horn (Saigae Tataricae Cornu, SAH) in the clinic and the pharmaceutical industry. In the present study, peptides released from SAH and GH under simulated gastric and intestinal digestion were identified. The results showed that most of the peptides released from SAH and GH under simulated gastrointestinal digestion were hydrophilic, and over 75% of the peptides from keratins (KRTs) were hydrophilic. In total, over 58% of the identified peptides were released from KRTs, and were from the four main regions of KRTs. The peptide features and the peptide release profiles from KRTs in SAH and GH were similar, which may provide a method for the identification of sustainable alternatives to replace the threatened SAH, and provide further evidence of the feasibility of using GH as a replacement for SAH based on their peptidomic analysis.
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http://dx.doi.org/10.1002/elps.201900078DOI Listing
October 2019

High Resolution Mass Profile of Bufadienolides and Peptides Combing with Anti-Tumor Cell Screening and Multivariate Analysis for the Quality Evaluation of Bufonis Venenum.

Molecules 2019 May 20;24(10). Epub 2019 May 20.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.

In order to evaluate the quality of Bufonis Venenum commercial herbs, a three-step qualitative and quantitative research study was performed. Firstly, we tried to identify small molecules and peptides in Bufonis Venenum using pre-fractionation chromatography and high-resolution mass spectrometry. The database search of the small molecules and peptides of Bufonis Venenum revealed that the dried venom consisted of free/conjugated-type bufadienolides and peptides with a mass range of 0.4-2 kDa. Secondly, we used partial least squares (PLS) multivariate statistical analysis to screen bufadienolides markers (VIP > 1.5) responsible for the anti-tumor cell activity of Bufonis Venenum, including 21 identified bufadienolides and 7 unknown compounds. It is noticeable that these bufadienolide markers could not be recognized by traditional HPLC-UV based spectrum-effect relationship analysis (correlation coefficient ranging from -0.24 to 0.40). Finally, we proposed a weight coefficient-based corrected total contents of 9 bufadienolides as a quality evaluation indicator, which had good correlation with inhibitory effects on tumor cells of commercial Bufonis Venenum. The correlation coefficient increased from 0.4 to 0.6. Thus, our pre-fractionation chromatography and mass spectrometry strategy had significant advancement over the traditional spectrum-effect relationship method for chemical marker identification. These results could be crucial and helpful in the development of a quality evaluation method that could reflect the pharmacological activity of Bufonis Venenum.
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http://dx.doi.org/10.3390/molecules24101943DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6572007PMC
May 2019