Publications by authors named "Zhenhua Zhao"

94 Publications

An Illustrated Guide to the Imaging Evolution of COVID in Non-Epidemic Areas of Southeast China.

Front Mol Biosci 2021 28;8:648180. Epub 2021 May 28.

Department of Radiology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

By analyzing the CT manifestations and evolution of COVID in non-epidemic areas of southeast China, analyzing the developmental abnormalities and accompanying signs in the early and late stages of the disease, providing imaging evidence for clinical diagnosis and identification, and assisting in judging disease progression and monitoring prognosis. This retrospective and multicenter study included 1,648 chest CT examinations from 693 patients with laboratory-confirmed COVID-19 infection from 16 hospitals of southeast China between January 19 and March 27, 2020. Six trained radiologists analyzed and recorded the distribution and location of the lesions in the CT images of these patients. The accompanying signs include crazy-paving sign, bronchial wall thickening, microvascular thickening, bronchogram sign, fibrous lesions, halo and reverse-halo signs, nodules, atelectasis, and pleural effusion, and at the same time, they analyze the evolution of the abovementioned manifestations over time. There were 1,500 positive findings in 1,648 CT examinations of 693 patients; the average age of the patients was 46 years, including 13 children; the proportion of women was 49%. Early CT manifestations are single or multiple nodular, patchy, or flaky ground-glass-like density shadows. The frequency of occurrence of ground-glass shadows (47.27%), fibrous lesions (42.60%), and microvascular thickening (40.60%) was significantly higher than that of other signs. Ground-glass shadows increase and expand 3-7 days after the onset of symptoms. The distribution and location of lesions were not significantly related to the appearance time. Ground-glass shadow is the most common lesion, with an average absorption time of 6.2 days, followed by consolidation, with an absorption time of about 6.3 days. It takes about 8 days for pure ground-glass lesions to absorb. Consolidation change into ground glass or pure ground glass takes 10-14 days. For ground-glass opacity to evolve into pure ground-glass lesions, it takes an average of 17 days. For ground-glass lesions to evolve into consolidation, it takes 7 days, pure ground-glass lesions need 8 days to evolve into ground-glass lesions. The average time for CT signs to improve is 10-15 days, and the first to improve is the crazy-paving sign and nodules; while the progression of the disease is 6-12 days, the earliest signs of progression are air bronchogram signs, bronchial wall thickening, and bronchiectasis. There is no severe patient in this study. This study depicts the CT manifestation and evolution of COVID in non-epidemic origin areas, and provides valuable first-hand information for clinical diagnosis and judgment of patient's disease evolution and prediction.
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http://dx.doi.org/10.3389/fmolb.2021.648180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195620PMC
May 2021

Quantitative perfusion histogram parameters of dynamic contrast-enhanced MRI to identify different pathological types of uterine leiomyoma.

Zhejiang Da Xue Xue Bao Yi Xue Ban 2021 02;50(1):97-105

Department of Radiology,Shaoxing People's Hospital,Shaoxing 312000,Zhejiang Province,China.

:To explore the value of quantitative perfusion histogram parameters of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in pathological classification of uterine leiomyoma and its correlation with Ki-67 protein expression. Thirty five patients with uterine leiomyoma confirmed by operation and pathology at Shaoxing People's Hospital from October 2015 to September 2017 were analyzed retrospectively,including 15 cases of ordinary type,8 cases of cellular type and 12 cases of degenerative type. All patients were examined by pelvic DCE-MRI before operation,and the histogram parameters (median,mean,skewness,kurtosis,energy,entropy) of various quantitative perfusion parameters,including volume transport constant (K),rate constant (K),extravascular extracellular space distribute volume per unit tissue volume (V),blood plasma volume per unit volume of tissue (V) were calculated,and the efficacy of different parameters in pathological classification of uterine leiomyoma was evaluated by ROC curve. The expression of Ki-67 protein in uterine leiomyoma was detected by immunohistochemical method,and the correlation between histogram parameters and Ki-67 protein expression was analyzed by Pearson and Spearman correlation analysis. The median and mean values of K,K,V and V in the cellular group were higher than those in the degenerative group and the ordinary group(<0.05 or <0.01),while the skewness of V,the skewness and kurtosis of K in the cellular group were lower than those in the ordinary group (all <0.05). The entropy of K in the cellular group was higher than that in the degenerative group and the ordinary group (all < 0.05). The entropy of V in the cellular group was higher than that in the ordinary group (<0.01). The median,mean,skewness of K,median and mean of K,median and mean of V,median,mean,energy and entropy of V were correlated with Ki-67 expression(all <0.05). The results of ROC curve analysis showed that the median threshold of K was 0.994/min,the sensitivity and specificity for the diagnosis of cellular uterine leiomyoma were 100.0% and 77.8% respectively,and the area under the ROC curve was 0.949. When the mean threshold of K was 1.170/min,the sensitivity and specificity for diagnosing cellular uterine leiomyoma were 100.0% and 77.8% respectively,and the area under the ROC curve was 0.958. The area under the ROC curve of K (entropy),K (median,mean),V (median,mean,entropy) in the diagnosis of cellular uterine leiomyoma were 0.755-0.907. :DCE-MRI quantitative perfusion histogram parameters have high diagnostic value in differentiating pathological types of uterine leiomyoma,especially for cellular uterine leiomyoma.
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http://dx.doi.org/10.3724/zdxbyxb-2021-0036DOI Listing
February 2021

Assessment of the water quality of groundwater in Bohai Rim and the controlling factors-a case study of northern Shandong Peninsula, north China.

Environ Pollut 2021 May 29;285:117482. Epub 2021 May 29.

College of Energy and Mining Engineering, Shandong University of Science and Technology, Qingdao, Shandong, 266590, China. Electronic address:

This study collected 184 groundwater (GW) samples from 92 wells during the dry and wet seasons, respectively to understand the hydrochemical characteristics, water quality, and risk of GW nitrate (NO) to human health in northern Shandong Peninsula (NSP), China. The results showed that GW in the NSP is weakly alkaline and classified as hard fresh water. The mean concentration of NO in GW exceeded 100 mg·L, total hardness exceeded 450 mg·L, and total dissolved solids (TDS) was less than 1000 mg·L. A Piper diagram showed that the water chemistry of GW in the NSP was mainly of the SO·Cl-Ca·Mg type. A Gibbs diagram and ion ratio analysis indicated that the weathering of silicate rocks and agricultural production were the dominant factors affecting the hydrochemical characteristics of GW in the NSP, with cation exchange, dissolution of salt rock, and weathering of carbonate rock also making contributions. Na and Cl in GW are significantly affected by seawater aerosols in coastal areas. During the wet season, the hydrodynamic conditions of the aquifer are improved, agricultural activities are strengthened, and GW becomes generally homogenized. The water quality index classified the GW quality of the NSP as mainly of medium quality. There was a relatively consistent spatial distribution of water quality during different periods. Water samples of poor water quality were mainly distributed in the lower reaches of the Huangshui River. In addition, total hardness and NO concentrations were the main variables affecting the quality of GW in the NSP. The assessment of the risk NO in GW in the NSP to human health through the ingestion of drinking water demonstrated a significant risk (infants > children > adults). These results indicate the need for local management measures to reduce the potential health risks of GW quality in the NSP.
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http://dx.doi.org/10.1016/j.envpol.2021.117482DOI Listing
May 2021

MRI-Based Radiomics Models for Predicting Risk Classification of Gastrointestinal Stromal Tumors.

Front Oncol 2021 10;11:631927. Epub 2021 May 10.

Department of Radiology, Shaoxing People's Hospital, Shaoxing Hospital, Zhejiang University School of Medicine, Shaoxing, China.

Background: We conduct a study in developing and validating four MRI-based radiomics models to preoperatively predict the risk classification of gastrointestinal stromal tumors (GISTs).

Methods: Forty-one patients (low-risk = 17, intermediate-risk = 13, high-risk = 11) underwent MRI before surgery between September 2013 and March 2019 in this retrospective study. The Kruskal-Wallis test with Bonferonni correction and variance threshold was used to select appropriate features, and the Random Forest model (three classification model) was used to select features among the high-risk, intermediate-risk, and low-risk of GISTs. The predictive performance of the models built by the Random Forest was estimated by a 5-fold cross validation (5FCV). Their performance was estimated using the receiver operating characteristic (ROC) curve, summarized as the area under the ROC curve (AUC). Area under the curve (AUC), accuracy, sensitivity, and specificity for risk classification were reported. Linear discriminant analysis (LDA) was used to assess the discriminative ability of these radiomics models.

Results: The high-risk, intermediate-risk, and low-risk of GISTs were well classified by radiomics models, the micro-average of ROC curves was 0.85, 0.81, 0.87 and 0.94 for T1WI, T2WI, ADC and combined three MR sequences. And ROC curves achieved excellent AUCs for T1WI (0.85, 0.75 and 0.82), T2WI (0.69, 0.78 and 0.78), ADC (0.85, 0.77 and 0.80) and combined three MR sequences (0.96, 0.92, 0.81) for the diagnosis of high-risk, intermediate-risk, and low-risk of GISTs, respectively. In addition, LDA demonstrated the different risk of GISTs were correctly classified by radiomics analysis (61.0% for T1WI, 70.7% for T2WI, 83.3% for ADC, and 78.9% for the combined three MR sequences).

Conclusions: Radiomics models based on a single sequence and combined three MR sequences can be a noninvasive method to evaluate the risk classification of GISTs, which may help the treatment of GISTs patients in the future.
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http://dx.doi.org/10.3389/fonc.2021.631927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141866PMC
May 2021

Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells.

Nat Commun 2021 05 20;12(1):2989. Epub 2021 May 20.

Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

The allogeneic transplantation of primordial germ cells (PGCs) derived from somatic cells overcomes the limitation of avian cloning. Here, we transdifferentiate chicken embryo fibroblasts (CEFs) from black feathered Langshan chickens to PGCs and transplant them into White Plymouth Rock chicken embryos to produce viable offspring with characteristics inherited from the donor. We express Oct4/Sox2/Nanog/Lin28A (OSNL) to reprogram CEFs to induced pluripotent stem cells (iPSCs), which are further induced to differentiate into PGCs by BMP4/BMP8b/EGF. DNA demethylation, histone acetylation and glycolytic activation elevate the iPSC induction efficiency, while histone acetylation and glycolytic inhibition facilitate PGCs formation. The induced PGCs (iPGCs) are transplanted into the recipients, which are self-crossed to produce 189/509 somatic cells derived chicken with the donor's characteristics. Microsatellite analysis and genome sequencing confirm the inheritance of genetic information from the donor. Thus, we demonstrate the feasibility of avian cloning from somatic cells.
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http://dx.doi.org/10.1038/s41467-021-23242-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138025PMC
May 2021

[Genetic screening and prenatal diagnosis in high-risk families with tuberous sclerosis complex syndrome].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 May;38(5):435-438

Center of Genetic and Prenatal Diagnosis, Department of Gynecology and Obstetrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.

Objective: To carry out genetic testing and prenatal diagnosis for 29 Chinese pedigrees affected with tuberous sclerosis complex (TSC) and assess efficacy of combined next generation sequencing (NGS) and multiple ligation-dependent probe amplification (MLPA) for the diagnosis.

Methods: NGS and MLPA were used in conjunct to detect variants of TSC1 and TSC2 genes among the probands of the pedigrees. Paternity test was carried out to exclude maternal DNA contamination. Prenatal diagnosis was provided to 14 couples based on the discoveries in the probands.

Results: Twenty-seven variants were identified in the TSC1 and TSC2 genes among the 29 pedigrees, which yielded a detection rate of 93.1%. Respectively, 5 (18.5%) and 22 (81.5%) variants were identified in the TSC1 and TSC2 genes. Twelve variants were unreported previously. Prenatal diagnosis showed that five fetuses were affected with TSC, whilst the remaining nine were unaffected.

Conclusion: Above finding has expanded the spectrum of TSC1 and TSC2 gene variants. Combined NGS and MLPA has enabled diagnosis of TSC with efficiency and accuracy.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200325-00203DOI Listing
May 2021

Correlation between quantitative perfusion histogram parameters of DCE-MRI and PTEN, P-Akt and m-TOR in different pathological types of lung cancer.

BMC Med Imaging 2021 Apr 17;21(1):73. Epub 2021 Apr 17.

Department of Radiology, The Second Affiliated Hospital of Zhejiang University, Hangzhou, 310009, China.

Background: To explore if the quantitative perfusion histogram parameters of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) correlates with the expression of PTEN, P-Akt and m-TOR protein in lung cancer.

Methods: Thirty-three patients with 33 lesions who had been diagnosed with lung cancer were enrolled in this study. They were divided into three groups: squamous cell carcinoma (SCC, 15 cases), adenocarcinoma (AC, 12 cases) and small cell lung cancer (SCLC, 6 cases). Preoperative imaging (conventional imaging and DCE-MRI) was performed on all patients. The Exchange model was used to measure the phar- macokinetic parameters, including K, V, K, V and F, and then the histogram parameters meanvalue, skewness, kurtosis, uniformity, energy, entropy, quantile of above five parameters were analyzed. The expression of PTEN, P-Akt and m-TOR were assessed by immunohistochemistry. Spearman correlation analysis was used to compare the correlation between the quantitative perfusion histogram parameters and the expression of PTEN, P-Akt and m-TOR in different pathological subtypes of lung cancer.

Results: The expression of m-TOR (P = 0.013) and P-Akt (P = 0.002) in AC was significantly higher than those in SCC. V (uniformity) in SCC group, K (uniformity), V (kurtosis, Q10, Q25) in AC group, F (skewness, kurtosis, energy), V (Q75, Q90, Q95) in SCLC group was positively correlated with PTEN, and F (entropy) in the SCLC group was negatively correlated with PTEN (P < 0.05); K (Q5, Q10) in the SCLC group was positively correlated with P-Akt, and K (energy) in the SCLC group was negatively correlated with P-Akt (P < 0.05); K (Q5) in SCC group and V (meanvalue, Q75, Q90, Q95) in SCLC group was positively correlated with m-TOR, and V (meanvalue) in SCC group was negatively correlated with m-TOR (P < 0.05).

Conclusions: The quantitative perfusion histogram parameters of DCE-MRI was correlated with the expression of PTEN, P-Akt and m-TOR in different pathological types of lung cancer, which may be used to indirectly evaluate the activation status of PI3K/Akt/mTOR signal pathway gene in lung cancer, and provide important reference for clinical treatment.
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http://dx.doi.org/10.1186/s12880-021-00604-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052821PMC
April 2021

Assisting scalable diagnosis automatically via CT images in the combat against COVID-19.

Sci Rep 2021 02 18;11(1):4145. Epub 2021 Feb 18.

Department of Radiology, Chinese PLA General Hospital, Beijing, 100853, People's Republic of China.

The pandemic of Coronavirus Disease 2019 (COVID-19) is causing enormous loss of life globally. Prompt case identification is critical. The reference method is the real-time reverse transcription PCR (RT-PCR) assay, whose limitations may curb its prompt large-scale application. COVID-19 manifests with chest computed tomography (CT) abnormalities, some even before the onset of symptoms. We tested the hypothesis that the application of deep learning (DL) to 3D CT images could help identify COVID-19 infections. Using data from 920 COVID-19 and 1,073 non-COVID-19 pneumonia patients, we developed a modified DenseNet-264 model, COVIDNet, to classify CT images to either class. When tested on an independent set of 233 COVID-19 and 289 non-COVID-19 pneumonia patients, COVIDNet achieved an accuracy rate of 94.3% and an area under the curve of 0.98. As of March 23, 2020, the COVIDNet system had been used 11,966 times with a sensitivity of 91.12% and a specificity of 88.50% in six hospitals with PCR confirmation. Application of DL to CT images may improve both efficiency and capacity of case detection and long-term surveillance.
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http://dx.doi.org/10.1038/s41598-021-83424-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892869PMC
February 2021

Clinical and Genetic Characteristics of 17 α-Hydroxylase/17, 20-Lyase Deficiency: c.985_987delTACinsAA Mutation of CYP17A1 Prevalent in the Chinese Han Population.

Endocr Pract 2021 Feb 8;27(2):137-145. Epub 2020 Dec 8.

Center of Genetic and Prenatal Diagnosis, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. Electronic address:

Objective: 17 α-hydroxylase/17, 20-lyase deficiency (17-OHD) is a rare recessive hereditary disease that can be attributed to cytochrome P450 17 α-hydroxylase deficiency caused by CYP17A1 gene mutations.

Methods: A large cohort of 10 Chinese Han patients with 17-OHD from 2012 to 2020 were enrolled. The clinical and biochemical features were investigated, and genetic mutations of CYP17A1 were analyzed by polymerase chain reaction-Sanger sequencing. Karyotype identification and the SRY gene test were also carried out. In silico analysis was used to predict the effects of genetic mutations on the protein function.

Results: All patients were female. Common complaints were hypertension, hypokalemia, and primary amenorrhea. The karyotype was 46, XY, and the SRY gene was detected in 7 patients; the karyotype was XX in the remaining 3 patients. A total of 7 mutations including Y329N, Y329X, Y329Lfs∗, R96W, A82D, S380N, and A487_P489del have been identified in the CYP17A1 gene. The Y329Lfs∗ mutation was found in 9/10 (90%) of patients with a high allele frequency of 70%. In silico prediction showed that a novel variant of c.1139G>A (S380N) occurs at a conserved residue and can cause disease.

Conclusion: We presented a detailed description of the clinical and genetic characteristics in Chinese patients with 17-OHD and concluded that Y329Lfs∗ mutation of CYP17A1 is prevalent in the Chinese Han population. Therefore, hotspot screening by polymerase chain reaction-Sanger sequencing for exon 6 of CYP17A1 could contribute to the rapid diagnosis of 17-OHD in China. Genetic counseling based on the genetic diagnosis for at-risk relatives is advised.
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http://dx.doi.org/10.4158/EP-2020-0478DOI Listing
February 2021

A Chinese child with hyperpigmentation diagnosed with familial glucocorticoid deficiency type 1 using whole-exome sequencing.

Pediatr Neonatol 2021 03 3;62(2):229-230. Epub 2020 Nov 3.

Center of Genetic and Prenatal Diagnosis, Zhengzhou University First Affiliated Hospital, Zhengzhou, China. Electronic address:

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http://dx.doi.org/10.1016/j.pedneo.2020.10.013DOI Listing
March 2021

Influence of Heat Treatment on Cyclic Response of Nickel-Based Superalloy Inconel 718 up to Very-High Cycle Regime.

Materials (Basel) 2020 Nov 26;13(23). Epub 2020 Nov 26.

Aero-Engine Thermal Environment and Structure Key Laboratory of Ministry of Industry and Information Technology, College of Energy and Power Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China.

Cyclic response and fatigue behavior are sensitive to the microstructure of material induced by heat treatment. In this paper, three sets of high-temperature superalloy Inconel 718 with different heat treatment, namely annealed, aged, and directly aged high quality (DAHQ), are compared. Difference in grain size distribution, phase, and precipitate, etc., were investigated using an optical camera and scanning electron microscopy. Yield and ultimate strength were found to increase obviously after aging heat treatment. Self-heating phenomenon at 20 kHz was attenuated as grain size decreased. There was a transition from cyclic hardening to softening. Very-high cycle fatigue (VHCF) behavior of Inconel 718 was tested using an ultrasonic fatigue device. Crack initiation duration occupied greater than 99% of the total fatigue life. It concluded that average grain size influences VHCF strength and crack initiation mechanism, and that self-heating phenomenon is not a decisive factor on VHCF strength for Inconel 718.
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http://dx.doi.org/10.3390/ma13235358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7730368PMC
November 2020

The environmental fate of phenanthrene in paddy field system and microbial responses in rhizosphere interface: Effect of water-saving patterns.

Chemosphere 2021 Apr 27;269:128774. Epub 2020 Oct 27.

Department of Geography and Earth Sciences, Aberystwyth University, Penglais, Aberystwyth, Ceredigion, SY23 3DB, UK.

The effects of water-saving patterns (Semi-dry water-saving, B; Shallow-wet control irrigation, Q; Traditional flooding irrigation, C; and Moistening irrigation, S) on the environmental fate of phenanthrene (Phe) and microbial responses in rhizosphere were investigated in paddy field system. Results showed the rice grain in Q treatment was more high production and safety with less Phe residue (up to 18%-49%) than other treatments, and the residual Phe in soil declined in the order: C (14.17%) > S (13.36%) > B (5.86%)>Q (2.70%), which proves the existence of optimal water conditions for PAHs degradation and rhizosphere effect during rice cultivation. Laccase (LAC) and dioxygenase (C23O) played important roles in Phe degradation, which were significantly positively correlated with Phe dissipation rate in soil (p < 0.01). Moreover, their activities in Q treatment, rhizosphere and subsoil were higher than those in C treatment, non-rhizoshere and upper layer soil. The introduction of Phe and rice into paddy field system decreased the microorganism diversity, and promoted the activities of enzymes and some PAHs degrading bacteria, such as Delftia, Serratia, Enterobacter, Pseudomonas, norank_f_Rhodospirillaceae, norank_f_Nitrosomonadaceae and so on. According to the cluster analysis, redundancy analysis and correlation analysis between bacterial community composition and environmental factors, water-saving patterns markedly impacted the relative abundance and bacterial community structure by the regulating and controlling on environmental conditions of paddy field. The dioxygenase activity, laccase activity, oxidation-reduction potential and conductivity were the main affecting factors on Phe dissipation during growth stage of rice.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128774DOI Listing
April 2021

Clinical Application of Oral Meglumine Diatrizoate Esophagogram in Screening for Esophageal Fistula During Radiotherapy or Chemoradiotherapy for Esophageal Cancer.

Front Oncol 2020 2;10:562147. Epub 2020 Oct 2.

Department of Oncology, Mianyang Central Hospital, Mianyang, China.

This study aimed to investigate the specificity and sensitivity of oral meglumine diatrizoate esophagogram in screening for esophageal fistula during radiotherapy or chemoradiotherapy for esophageal cancer and determine if early detection and intervention could improve the prognosis of esophageal fistulas. Esophageal cancer patients undergoing radiotherapy or chemoradiotherapy were included. Weekly oral meglumine diatrizoate esophagograms were performed to screen for esophageal fistulas during radiotherapy. When an esophageal fistula was detected, fibroesophagoscopy and computed tomography (CT) were used for confirmation; once confirmed, radiotherapy was discontinued, and the patient received intervention. The esophagogram results were reviewed weekly to assess the recovery of the esophageal fistula. If the fistula was healed, the patient resumed and completed radiotherapy. A total of 206 patients with cancer of the esophagus undergoing chemotherapy/radiotherapy were included. During radiotherapy, 10 cases of esophageal fistula were detected or suspected based on the oral meglumine diatrizoate esophagography findings, and eight of those cases were confirmed by CT and esophagoscopy. All patients with esophageal fistula received intervention; among them, 62.5% (5/8) recovered after 1 to 2 weeks of treatment and continued radiotherapy to completion. The sensitivity and specificity of oral meglumine diatrizoate esophagography in screening for esophageal fistulas during radiotherapy or chemoradiotherapy were 100 and 98.9%, respectively. The median survival period of patients with esophageal fistulas was 6.4 months. Oral meglumine diatrizoate esophagography has high sensitivity and specificity in screening for esophageal fistulas during radiotherapy or chemoradiotherapy with minimal side effects. Early diagnosis and timely intervention can significantly improve the prognosis and prolong the survival period of patients. Chictr.org.cn, Identifier: ChiCTR-DDD-17012617. Registered on September 7, 2017. The first participant was enrolled on September 25, 2017. http://www.chictr.org.cn/showproj.aspx?proj=21526.
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http://dx.doi.org/10.3389/fonc.2020.562147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566907PMC
October 2020

Temporal changes of COVID-19 pneumonia by mass evaluation using CT: a retrospective multi-center study.

Ann Transl Med 2020 Aug;8(15):935

Department of Radiology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Coronavirus disease 2019 (COVID-19) has widely spread worldwide and caused a pandemic. Chest CT has been found to play an important role in the diagnosis and management of COVID-19. However, quantitatively assessing temporal changes of COVID-19 pneumonia over time using CT has still not been fully elucidated. The purpose of this study was to perform a longitudinal study to quantitatively assess temporal changes of COVID-19 pneumonia.

Methods: This retrospective and multi-center study included patients with laboratory-confirmed COVID-19 infection from 16 hospitals between January 19 and March 27, 2020. Mass was used as an approach to quantitatively measure dynamic changes of pulmonary involvement in patients with COVID-19. Artificial intelligence (AI) was employed as image segmentation and analysis tool for calculating the mass of pulmonary involvement.

Results: A total of 581 confirmed patients with 1,309 chest CT examinations were included in this study. The median age was 46 years (IQR, 35-55; range, 4-87 years), and 311 (53.5%) patients were male. The mass of pulmonary involvement peaked on day 10 after the onset of initial symptoms. Furthermore, the mass of pulmonary involvement of older patients (>45 years) was significantly severer (P<0.001) and peaked later (day 11 day 8) than that of younger patients (≤45 years). In addition, there were no significant differences in the peak time (day 10 day 10) and median mass (P=0.679) of pulmonary involvement between male and female.

Conclusions: Pulmonary involvement peaked on day 10 after the onset of initial symptoms in patients with COVID-19. Further, pulmonary involvement of older patients was severer and peaked later than that of younger patients. These findings suggest that AI-based quantitative mass evaluation of COVID-19 pneumonia hold great potential for monitoring the disease progression.
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http://dx.doi.org/10.21037/atm-20-4004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475384PMC
August 2020

Fat mass- and obesity-associated (FTO) gene promoted myoblast differentiation through the focal adhesion pathway in chicken.

3 Biotech 2020 Sep 25;10(9):403. Epub 2020 Aug 25.

Institute of Poultry Science, Chinese Academy of Agriculture Sciences, Jiangsu, 225125 People's Republic of China.

The action of on myoblasts proliferation and differentiation and molecular mechanism underlying it were investigated by transfecting with lentiviral overexpression vector and gene expression profile sequencing. Compared with the control group, myoblasts with transfection was significantly enhanced proliferation; the expression of and was significantly increased. In cells transfected with , 129 differentially expressed genes were determined compared with control group, with 104 up-regulated and 25 down-regulated genes. Twelve pathways (Phagosome, Focal adhesion, Adrenergic signaling in cardiomyocytes, Endocytosis, Cardiac muscle contraction, Toll-like receptor, Ribosome, Tight junction, Regulation of actin cytoskeleton, Cytokine-cytokine receptor interaction, Adrenergic signaling in cardiomyocytes and MAPK) were significantly enriched. Eight genes known to be directly or indirectly related to skeletal muscle development (, , , , , , and ) were enriched in the focal adhesion and expressed differentially. Altogether, these data suggested that stimulated differentiation of myoblasts through regulation of eight genes enriched in the focal adhesion.
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http://dx.doi.org/10.1007/s13205-020-02386-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447686PMC
September 2020

Circ-SAR1A Promotes Renal Cell Carcinoma Progression Through miR-382/YBX1 Axis.

Cancer Manag Res 2020 18;12:7353-7361. Epub 2020 Aug 18.

Department of Urology, Huaihe Hospital of Henan University, Kaifeng 475000, People's Republic of China.

Background: Accumulating evidence points to a role for circular RNAs (circRNAs) in important regulatory function in tumor advancement. We explored the effect and function of circ-SAR1A in renal cell carcinoma (RCC).

Methods: circ-SAR1A expression in RCC tissues and cell lines was explored by qRT-PCR. The roles of circ-SAR1A on RCC progression were explored by in vitro function assays. Moreover, we determined the underlying mechanism of circ-SAR1A in RCC progression through bioinformatics analysis and dual-luciferase reporter assays.

Results: Our data reveal that circ-SAR1A is significantly high in RCC tissues and cell lines. High circ-SAR1A levels are correlated to advanced Fuhrman grade, and lymph-node metastasis in RCC patients. Functional experiments indicate that circ-SAR1A suppression decreased RCC cell growth and invasion abilities in vitro. Mechanistically, circ-SAR1A upregulated YBX1 expression by sponging miR-382, resulting in promoting the growth and invasion in RCC cells.

Conclusion: Our data indicate that the circ-SAR1A/miR-382/YBX1 axis plays a critical role in RCC progression, which serve as a potential novel treatment strategy of RCC.
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http://dx.doi.org/10.2147/CMAR.S245918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443449PMC
August 2020

Long-term results of the edge-to-edge repair for failed mitral valve repair as a bailout option.

Gen Thorac Cardiovasc Surg 2021 Jan 11;69(1):32-37. Epub 2020 Jul 11.

Department of Cardiovascular Surgery, Fuwai Hospital, Chinese Academy of Medical Science, No. 167, North Lishi Road, Xicheng District, Beijing, 100037, China.

Objective: We evaluate the long-term clinical and echocardiographic outcomes of edge-to-edge (E2E) repair combined with mitral annuloplasty in our institute with degenerative mitral regurgitation (MR) up to 10 years.

Methods: Twenty-six consecutive patients undergoing E2E mitral repair after failure of other conventional mitral repair technique. There were 16 (61.5%) male and the mean age was 52.2 ± 10.4 years. Mitral regurgitation was due to anterior leaflet prolapse in 12 (46.2%) patients, bileaflet prolapse in 9 (34.6%), and multiple segment posterior leaflet prolapse in 5 (19.2%).

Results: There were no perioperative deaths. Follow-up was 100% complete. The mean length of follow-up was 8.7 ± 0.9 years (median 8.4 years, 6.8-10.1 years). Two patients required reoperation of the MV. The freedom from reoperation and ≥ Moderate MR at 10 years was 69.9 ± 11.7%. The freedom from reoperation, ≥ Moderate MR and mitral stenosis (MS) at 10 years was (59.6 ± 10.0) %. The mean transmitral pressure gradient (TMPG) was 6.1 ± 2.5 mmHg, which was significantly elevated compared with preoperative TTE (P = 0.004). The freedom from ≥ Moderate MS at 10 years was 76.9 ± 8.3%. There were no differences between patients with and without MS at follow-up regarding the echocardiographic parameters and clinical status.

Conclusion: There is a slowly progressive elevation of TMPG after E2E mitral repair, while mildly elevated TMPG can be tolerated in most patients. In appropriate patients, the E2E repair combined with annuloplasty provides an effective "bailout" choice.
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http://dx.doi.org/10.1007/s11748-020-01433-zDOI Listing
January 2021

A safe and novel method for video-assisted thoracic surgery preoperative localization of small pulmonary nodules by using ZT medical glue (2-octyl cyanoacrylate).

Surg Oncol 2020 Jun 18;33:164-169. Epub 2020 Feb 18.

Department of Cardiothoracic Surgery, Shaoxing People's Hospital (Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing, Zhejiang Province, China. Electronic address:

Background: Accurate and fast localization of small pulmonary nodules is required for local pulmonary resection. In this study, we introduced and assessed a novel technique for the preoperative localization of small pulmonary nodules by using ZT medical glue (2-octyl cyanoacrylate).

Methods: 101 patients who had a combined total of 106 small pulmonary nodules located by ZT glue and 53patients with 53 small pulmonary nodules located by hookwire were selected. Guided by computed tomography (CT), the surgeon injected certain volume ZT glue into an area adjacent to the small pulmonary nodule, then, the adjacent lung tissue infiltrated by ZT glue formed into a depressed hard nodule which can be used for preoperative localization with an obvious mark on lung surface or different hand touch. After localization, Wedge resection was performed via video-assisted thoracoscopic surgery and the specimen obtained from the procedure was immediately sent for pathological examination, followed by a standard surgical procedure. A contrast has been made between the ZT glue method and the hookwire.

Results: 101 operations were successfully performed by using this novel technique, and 106 small pulmonary nodules were successfully located. Compared with the hookwire location, ZT glue method obviouslyextended the Time interval between localization and operation (P = 0.00) and a same complication rate (P = 0.07).

Conclusions: The use of ZT glue is a safe and effective method for the localization of small pulmonary nodules.

Trial Registration: This study was approved by the ethics committee of Shaoxing People's Hospital (Number:2016-004, Date:2016,2,24), and informed consent was obtained from all enrolled patients.
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http://dx.doi.org/10.1016/j.suronc.2020.02.001DOI Listing
June 2020

Mutation analysis of 419 family and prenatal diagnosis of 339 cases of spinal muscular atrophy in China.

BMC Med Genet 2020 06 18;21(1):133. Epub 2020 Jun 18.

The Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, Add: No. 1, Jianshe East Rd, Erqi District, Zhengzhou, Henan Province, China.

Background: Spinal muscular atrophy (SMA) is a common and lethal autosomal recessive neurodegenerative disease caused by mutations in the survival motor neuron 1 (SMN1) gene. At present, gene therapy medicine for SMA, i.e., Spinraza (Nusinersen), has been approved by the FDA, bringing hope to SMA patients and families. Accurate diagnosis is essential for treatment. Our goal was to detect genetic mutations in SMA patients in China and to show the results of the prenatal diagnosis of SMA.

Methods: In this study, we examined 419 patients in our hospital from January 2010 to September 2019. Multiplex ligation-dependent probe amplification analysis was used to determine the copy numbers of SMN1 and SMN2. Long-range PCR combined with nested PCR was used to detect point mutations in SMN1. In addition to the above detection methods, we also used QF-PCR in prenatal diagnosis to reduce the impact of maternal contamination. We conducted a total of 339 prenatal diagnoses from January 2010 to September 2019.

Results: Homozygous deletion of SMN1 exon 7 was detected in 96.40% (404/419) of patients. Homozygous deletion of SMN1 exon 7 alone was detected in 15 patients (3.60%). In total, 10 point mutations were detected in the 15 pedigrees. Most patients with SMA Type I have 1 ~ 2 copies of the SMN2 gene. Patients with SMA Type II have 2 or 3 copies of the SMN2 gene. The results of prenatal diagnoses showed that 118 fetuses were normal, 149 fetuses were carriers of heterozygous variants, and the remaining 72 fetuses harbored compound heterozygous variants or homozygous variants.

Conclusions: Our study found that the most common mutation in SMA was homozygous deletion of SMN1 exon 7 in our study. We suggest that detecting only the deletion of exon 7 of SMN1 can meet most of the screening needs. We also believe that SMN2 copy numbers can help infer the disease classification and provide some reference for future treatment options.
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http://dx.doi.org/10.1186/s12881-020-01069-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302341PMC
June 2020

Study on the dynamic change law and correlation between CT imaging manifestations and cellular immunity of 2019 novel coronavirus disease.

Jpn J Radiol 2020 Oct 12;38(10):942-952. Epub 2020 Jun 12.

Department of Radiology, Shaoxing People's Hospital (Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing, Zhejiang, China.

Purpose: To explore the dynamic changes and correlation between CT imaging manifestations and cellular immunity of COVID-19.

Materials And Methods: This retrospective review analyzed 23 patients with COVID-19, including 13 males and 10 females aged 27-70 years, with an average age of 48 years. Patients were divided into two groups: group A with 11 critical-severe patients, and group B with 12 common-mild patients. Clinical, laboratory, and radiological data were collected and analyzed.

Results: LYM, LYM (%), CD3+, CD4+, and CD8+ decreased, while NEU (%), CRP, and CT scores increased in all patients, WBC in group A increased. In group A, on day 10-12 after disease onset, CT scores and CRP reached the highest point, and day 13-15 LYM, LYM (%) reached the lowest but NEU (%) and WBC reached the highest, CD3+, CD4+ and CD8+ were at the lowest on day 10-15. In group B, on day 7-9, CT scores, NEU (%) and CRP reached the peak, but LYM, LYM (%), CD3+, CD4+ and CD8+ reached the lowest. In all patients, CT scores had a significantly negative correlation with CD3+, CD4+, CD8+, LYM (%), and LYM (p = 0.001, r = - 0.797; p = 0.008, r = - 0.698; p = 0.002, r = - 0.775; p < 0.001, r = - 0.785; p = 0.021, r = - 0.571, respectively), and a significantly positive correlation with WBC and NEU (%) (p < 0.001, r = 0.785; p = 0.003, r = 0.691, respectively).

Conclusion: Dynamic changes of CT manifestations and cellular immunity of patients with COVID-19 were regular and correlation was high between these two parameters.
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http://dx.doi.org/10.1007/s11604-020-00997-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289933PMC
October 2020

Coupled photocatalytic-bacterial degradation of pyrene: Removal enhancement and bacterial community responses.

Environ Res 2020 04 13;183:109135. Epub 2020 Jan 13.

Jiangsu Rainfine Environmental Science and Technology Co., Ltd, Nanjing, 210009, China.

Polycyclic aromatic hydrocarbons (PAHs) are a class of pollutants that ubiquitously present in environment and hard to be degraded by microorganisms. Herein, we reported a novel photocatalytic-bacterial coupled removal system to treat PAH-polluted water. Using pyrene as the model pollutant, we demonstrated that the removal percentage of different groups was in order: 63.89% ± 1.03% (Vis-Biological) > 61.27% ± 1.08% (UV-Biological) > 59.58% ± 1.15% (UV) > 57.41% ± 1.13% (Vis) > 6.65% ± 0.72% (Biological) > 1.70% ± 0.34% (Control), showing the coupled system significantly improved the removal percentage of pyrene. Additionally, we observed that the coupled system driven by visible light showed higher removal percentage than UV light, exhibiting a good potential for future application. Sequencing analysis of 16S rRNA genes showed that alpha diversity (richness, evenness and diversity) got promoted and data of the relative abundance showed that Pseudomonadaceae was substituted as the dominant bacteria for Planococcaceae, with some other functional bacteria quickly acclimatizing in the bacterial community. Difference analysis indicated that over half of top fifteen genera were generally different significantly (p < 0.001) among two different samples, and UV light altered structure and composition of bacterial community more than visible light. Functional features' change suggested that the bacterial community not only protected itself but also participated in degrading pyrene. Overall, our study offered a new method for PAH degradation and contributed to further understanding of coupled catalytic-bacterial degradation processes.
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http://dx.doi.org/10.1016/j.envres.2020.109135DOI Listing
April 2020

Phenanthrene removal and response of bacterial community in the combined system of photocatalysis and PAH-degrading microbial consortium in laboratory system.

Bioresour Technol 2020 Apr 7;301:122736. Epub 2020 Jan 7.

Jiangsu Rainfine Environmental Science and Technology Co., Ltd, Nanjing 210009, China.

This work aimed to study the removal of phenanthrene, change of bacterial community and microbial functions through combined photocatalysis and biodegradation under ultraviolet (UV) and visible light illumination. Results showed that phenanthrene removal was enhanced in combined system irradiated by UV and visible light. High-throughput sequencing of 16S rRNA gene manifested that alpha diversity (richness, evenness and diversity) got promoted and data of relative abundance reported that Planococcaceae as the dominant bacteriawas replaced by Pseudomonadaceae, with some other functional bacteria quickly acclimatizing. Difference analysis indicated that top fifteen genera were generally different significantly (p < 0.001) among two distinct samples, particularly for Pseudomonas on relative abundance. Functional features' regulation suggested that the bacterial community not only protected itself well but also participated in degrading phenanthrene. Selecting suitable degrading microbial consortium from natural environment and understanding deeply on performance of bacterial community contribute to assemble effective and directional combined system.
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http://dx.doi.org/10.1016/j.biortech.2020.122736DOI Listing
April 2020

[Value of myocardial scar in predicting malignant ventricular arrhythmia in patients with chronic myocardial infarction].

Zhejiang Da Xue Xue Bao Yi Xue Ban 2019 Jul;48(5):511-516

Department of Radiology, Shaoxing People's Hospital, Shaoxing 312000, Zhejiang Province, China.

Objective: To assess the predictive value of myocardial scar mass in malignant ventricular arrhythmia (MVA) after myocardial infarction.

Methods: Thirty myocardial infarction patients with complete electrophysiology and cardiac MRI data admitted from January 2012 to August 2017 were enrolled in the study. According to the results of intracavitary electrophysiological study, MVA developed in 16 patients (MVA group) and not developed in 14 patients (non-MVA group). The qualitative and quantitative analysis of left ventricular ejection fraction (LVFE) and scar mass was performed with CV post-processing software and predictive value of myocardial scar and LVEF for MVA after myocardial infarction was analyzed using ROC curves.

Results: LVEF in MVA group was significantly lower than that in non-MVA group, and scar mass in MVA group was significantly higher than that in non-MVA group (all <0.05). Regression analysis showed that LVEF (=1.580) and scar mass (=6.270) were risk factors for MVA after myocardial infarction. For predicting MVA, the area under ROC curve () of LVEF was 0.696 with a sensitivity of 0.786 and the specificity of 0.685; the of the scar mass was 0.839 with a sensitivity was 0.618 and the specificity of 0.929; the of LVEF combined with scar mass was 0.848 with a sensitivity of 0.688 and specificity of 0.857.

Conclusions: Myocardial scar assessed by late gadolinium enhancement MRI is more effective than LVEF in predicting MVA after myocardial infarction.
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July 2019

[Prenatal diagnosis for two families affected with cleidocranial dysplasia due to novel RUNX2 variants].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2019 Dec;36(12):1179-1182

Genetics and Prenatal Diagnosis Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.

Objective: To analyze variants of RUNX2 gene in two pedigrees affected with cleidocranial dysplasia and provide prenatal diagnosis for them.

Methods: For the two probands, the coding sequences of the RUNX2 gene were analyzed with PCR and bidirectional Sanger sequencing. To verify the results, peripheral blood samples were collected from their parents and 100 healthy controls. For family 1, umbilical cord blood was also collected for prenatal genetic diagnosis.

Results: In family 1, the proband and the fetus both carried a heterozygous c.578G>C (p.Arg193Pro) mutation. For family 2, the proband was found to carry a heterozygous c.909C>A (p.Tyr303X) mutation. The same mutations were not found among their parents and 100 healthy controls. Neither mutation was reported previously.

Conclusion: Variants of the RUNX2 gene probably underlie the cleidocranial dysplasia in both pedigrees. The results enabled prenatal diagnosis for the affected family.
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http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2019.12.007DOI Listing
December 2019

Influence of Age on Clinical Presentation, Therapeutic Options, and Outcome in Chinese Patients with Acute Aortic Dissection.

Int Heart J 2019 Nov 31;60(6):1373-1380. Epub 2019 Oct 31.

State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College.

It has been shown in previous studies that Chinese patients with acute aortic dissection (AD) were approximately 10 years younger than patients from western countries. However, there is a lack of studies concerning the age-related differences in clinical characteristics and outcomes in Chinese patients with acute AD. A total of 1,061 patients with AD (570 type A and 491 type B AD) were enrolled between 2006 and 2008. The clinical characteristics were compared between the patients in our study and those in the International Registry of Acute Aortic Dissection (IRAD). Compared with patients in the IRAD, those in our study were relatively younger, comprised more males, and had a higher proportion of Marfan syndrome but received fewer surgical interventions. When stratified by 10-year age, younger patients were more likely to have type A AD, familial AD, and Marfan syndrome, whereas older patients tended to comprise more females and type B AD. As age increased, the proportion of surgical intervention gradually decreased regardless of the type of AD. During a median follow-up of 2.2 years, 147 patients died, of whom 94 (63.9%) had type A AD and 53 (36.1%) had type B AD. Long-term mortality increased with increasing age, especially in patients above 70 years old. Furthermore, the recurrence rate of AD was higher in both the young and the older patients. In conclusion, compared with western patients with AD, Chinese patients have distinct characteristics and more attention should be paid to the young and older patients because of their high long-term mortality and recurrence rate.
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http://dx.doi.org/10.1536/ihj.18-532DOI Listing
November 2019

Correction to: Cu/N-codoped TiO prepared by the sol-gel method for phenanthrene removal under visible light irradiation.

Environ Sci Pollut Res Int 2019 Dec;26(35):36073

School of Civil Engineering, Southeast University, Nanjing, 210096, China.

The article "Cu/N-codoped TiO prepared by the sol-gel method for phenanthrene removal under visible light irradiation," written by Zhenhua Zhao, Abduelrahman Adam Omer, Zhirui Qin, Salaheldein Osman, Liling Xia, and Rajendra Prasad Singh.
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http://dx.doi.org/10.1007/s11356-019-06541-9DOI Listing
December 2019

Cu/N-codoped TiO prepared by the sol-gel method for phenanthrene removal under visible light irradiation.

Environ Sci Pollut Res Int 2020 May 17;27(15):17530-17540. Epub 2019 Jul 17.

School of Civil Engineering, Southeast University, Nanjing, 210096, China.

Cu/N-codoped TiO nanoparticles were prepared by the modified sol-gel method, to study its efficiency for the removing of polyaromatic hydrocarbon (phenanthrene) from an aqueous solution. Urea and copper sulfate pentahydrate were used as sources of doping element for Cu/N-codoped TiO, respectively. The characterizations of the nanoparticles were done by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and UV-vis diffuse reflectance spectra. XRD revealed that all the nanoparticles were indexed to the anatase phase structure, with crystallite size range from 11 to 30 nm, which decreased with the doping of copper and nitrogen. The photocatalytic activities of Cu/N-codoped TiO showed the highest activities than other TiO nanoparticles (TiO and N-doped TiO). The photodegradation efficiency of Cu/N-codoped TiO on phenanthrene under visible light irradiation was slightly higher (96%) comparing to UV light irradiation (94%). Cu/N-codoped TiO was found to be very efficient and economical for phenanthrene removal, because the smallest amount of Cu/N-codoped TiO exhibited the best removal efficiency on phenanthrene.
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http://dx.doi.org/10.1007/s11356-019-05787-7DOI Listing
May 2020

The dissipation and risk alleviation mechanism of PAHs and nitrogen in constructed wetlands: The role of submerged macrophytes and their biofilms-leaves.

Environ Int 2019 10 5;131:104940. Epub 2019 Jul 5.

Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824, USA.

The role of submerged macrophytes (Vallisneria natans, Hydrilla verticillata and artificial plant) and their biofilms-leaves for the dissipation and risk alleviation mechanism of PAHs (phenanthrene and pyrene) and nitrogen in constructed wetland systems with PAH-polluted sediments were investigated. Biofilms-leaves/surface might contribute to PAHs degradation, which was positively correlated with PAHs degrading bacteria. Nitrogen-fixing bacteria in biofilms on surface might cause total nitrogen in sediment (TNs) increasing by 4% from 14th d to 28th d indirectly when suffering PAHs pollution. The relative abundance of nitrogen-fixing bacteria significantly increased with the increase of PAHs concentrations in early period (p < 0.01), which might lead to risk of nitrogen accumulation further. Heat maps showed that the relative abundance of functional bacteria were influenced in order of attached surface > incubation time > spiking concentration of PAHs. Interestingly, differences of deduced bacterial functions were affected in order of incubation time > attached surface > spiking concentration. Thus, submerged macrophytes and their biofilms on leaves not only played an important role in PAHs degradation, but also regulated the nitrogen cycling in constructed wetland systems, which could reduce these pollutants risk for natural environment, organisms and human health.
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http://dx.doi.org/10.1016/j.envint.2019.104940DOI Listing
October 2019

Safety and efficacy of pulsed low-dose rate radiotherapy for local recurrent esophageal squamous cell carcinoma after radiotherapy: Study protocol for a prospective multi-center phase II trial.

Medicine (Baltimore) 2019 Jun;98(26):e16176

Department of Oncology, Mian Yang Central Hospital, Mianyang.

Introduction: Re-irradiation after radiotherapy is a common treatment for locally recurrent esophageal cancer. However, the side effects of re-irradiation are serious. The most serious adverse reactions of re-irradiation include esophageal perforation and hemorrhage caused by esophageal perforation. Studies have shown that pulsed low-dose rate radiotherapy (PLDR) induces a hypersensitivity effect on tumor tissue and a hyper-repair effect on normal tissue, which can simultaneously reduce damage on the normal tissue and increase the therapeutic effect on the tumor. The objective of this study is to explore whether PLDR can reduce rate of esophageal perforation and improve efficacy in patients with recurrent esophageal squamous cell carcinoma (ESCC) after radiotherapy.

Methods And Analysis: This study is a prospective, multi-center, open, single-arm clinical trial designed to enroll 27 patients with locally recurrent ESCC after radiotherapy with or without chemotherapy. Re-irradiation will be performed using intensity modulated radiation therapy in 50 Gy/25 fractions. The strategy of PLDR includes dividing 2 Gy into 10 fractions, and administering each irradiating dose of 20 cGy at an interval of 3 minutes before the next low-dose irradiation. The actual dose rate of administration each time will be 16.67 cGy /minute. The primary endpoint in this study is the rate of esophageal perforation. The secondary endpoints are the objective remission rate, the palliative effect on quality of life and pain, and the time of disease progression. The observation time is 2 years after the end of the study.

Trial Registration: Clinical trial number: ChiCTR1900020609.
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http://dx.doi.org/10.1097/MD.0000000000016176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6620741PMC
June 2019

[Analysis of ADAR gene mutations in two pedigrees affected with dyschromatosis symmetrica hereditaria].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2019 Jun;36(6):574-576

Genetics and Prenatal Diagnosis Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China. Email:

Objective: To detect mutations of ADAR gene in two pedigrees affected with dyschromatosis symmetrica hereditaria (DSH).

Methods: Potential mutations of the ADAR gene were analyzed by Sanger sequencing of the probands from both pedigrees. Suspected mutations were validated by Sanger sequencing of other patients from both pedigrees as well as unrelated healthy individuals.

Results: A heterozygous nonsense mutation c.1325C>G (p.Ser442Ter) and a novel nonsense mutation c.1498C>T (p.Gln500Ter) were respectively identified in the ADAR gene among all patients from the two pedigrees but not among 200 healthy individuals.

Conclusion: Mutations of the ADAR gene probably underlie the DSH in the two pedigrees. Above findings have enriched the spectrum of ADAR gene mutation.
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http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2019.06.010DOI Listing
June 2019
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