Publications by authors named "Zhenhua Wang"

319 Publications

Modified lung ultrasound scoring system to evaluate the feasibility of pregnant women with COVID-19 pneumonia.

J Matern Fetal Neonatal Med 2021 Jun 6:1-6. Epub 2021 Jun 6.

Department of Ultrasound Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.

Objective: To investigate whether physicians with short-term training can use a modified lung ultrasound scoring system for coronavirus disease 2019 (COVID-19) pneumonia to assess lung damage in pregnant women.

Methods: Sixteen consecutively hospitalized third-trimester pregnant women with pregnancy-induced hypertension, preeclampsia, rheumatoid arthritis or connective tissue disease were selected as the study subjects for the simulation of COVID-19 pneumonia. Two physicians (imaging and internal medicine) without ultrasonic experience performed lung examinations on pregnant women after six days of lung ultrasound training, and their consistency with examinations by the expert was assessed. In addition, 54 healthy third-trimester pregnant women and 54 healthy nonpregnant women of the same age who were continuously treated in the outpatient clinic of this hospital were selected for comparisons of abnormalities on lung ultrasound.

Results: (1) Third trimester pregnant women with pregnancy-induced hypertension, preeclampsia, rheumatoid arthritis or connective tissue disease had the same lung ultrasound patterns as those associated with COVID-19 pneumonia. (2) There was no statistically significant difference between the scores of the two trained doctors and the expert when the modified ultrasound scoring system was used ( > .05). (3) The evaluations of the two trained doctors and the expert showed good consistency (kappa value = 0.833-0.957). (4) The incidence of abnormal ultrasound manifestations of the pleura and lung parenchyma was higher among healthy third-trimester pregnant women than among healthy women of the same age ( < .001).

Conclusions: After receiving short-term training, imaging and internal medicine physicians can use the modified lung ultrasound scoring system to evaluate pregnant women's pulmonary damage, but caution is needed to avoid false-positive results among pregnant women with suspected COVID-19 pneumonia.
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http://dx.doi.org/10.1080/14767058.2021.1874912DOI Listing
June 2021

Integrated Gene Co-expression Analysis and Metabolites Profiling Highlight the Important Role of ZmHIR3 in Maize Resistance to Stalk Rot.

Front Plant Sci 2021 11;12:664733. Epub 2021 May 11.

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

stalk rot (GSR) caused by is one of the most devastating diseases causing significant yield loss of maize, and GSR resistance is a quantitative trait controlled by multiple genes. Although a few quantitative trait loci/resistance genes have been identified, the molecular mechanisms underlying GSR resistance remain largely unexplored. To identify potential resistance genes and to better understand the molecular mechanism of GSR resistance, a joint analysis using a comparative transcriptomic and metabolomic approaches was conducted using two inbred lines with contrasting GSR resistance, K09 (resistant) and A08 (susceptible), upon infection with . While a substantial number of differentially expressed genes associated with various defense-related signaling pathways were identified between two lines, multiple hub genes likely associated with GSR resistance were pinpointed using Weighted Gene Correlation Network Analysis and K-means clustering. Moreover, a core set of metabolites, including anthocyanins, associated with the hub genes was determined. Among the complex co-expression networks, showed strong correlation with multiple key genes, and genetic and histological studies showed that mutant is more susceptible to GSR, accompanied by enhanced cell death in the stem in response to infection with . Taken together, our study identified differentially expressed key genes and metabolites, as well as co-expression networks associated with distinct infection stages of . Moreover, likely plays a positive role in disease resistance to GSR, probably through the transcriptional regulation of key genes, functional metabolites, and the control of cell death.
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http://dx.doi.org/10.3389/fpls.2021.664733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144520PMC
May 2021

The structural mechanism for the nucleoside tri- and diphosphate hydrolysis activity of Ntdp from Staphylococcus aureus.

FEBS J 2021 May 6. Epub 2021 May 6.

National Synchrotron Radiation Laboratory, Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, China.

Staphylococcus aureus is a well-known clinical pathogenic bacterium. In recent years, due to the emergence of multiple drug-resistant strains of S. aureus in clinical practice, S. aureus infections have become an increasingly severe clinical problem. Ntdp (nucleoside tri- and diphosphatase, also known as Sa1684) is a nucleotide phosphatase that has a significant effect on the proliferation of S. aureus colonies and the killing ability of the host. Here, we identified the nucleoside tri- and diphosphate hydrolysis activity of Ntdp and obtained the three-dimensional structures of apo-Ntdp and three substrate analog (ATP S, GDP S, and GTP S) complexes of Ntdp. Through structural analysis and biochemical verification, we illustrated the structural basis for the divalent cation selectivity, substrate recognition model, and catalytic mechanism of Ntdp. We also revealed a possible basal functional pattern of the DUF402 domain and hypothesized the potential pathways by which the protein regulates the expression of the two-component regulatory factor agr and the downstream virulence factors. Overall, the above findings provide crucial insights into our understanding of the Ntdp functional mechanism in the infection process.
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http://dx.doi.org/10.1111/febs.15911DOI Listing
May 2021

Xanthohumol-Induced Rat Glioma C6 Cells Death by Triggering Mitochondrial Stress.

Int J Mol Sci 2021 Apr 26;22(9). Epub 2021 Apr 26.

Center for Mitochondria and Healthy Aging, College of Life Science, Yantai University, Yantai 264005, China.

Aim: To investigate the underlying mechanisms of xanthohumol (XN) on the proliferation inhibition and death of C6 glioma cells.

Methods: To determine the effects of XN on C6 cells, cell proliferation and mortality after XN treatment were assessed by SRB assay and trypan blue assay respectively. Apoptotic rates were evaluated by flowcytometry after Annexin V-FITC/PI double staining. The influence of XN on the activity of caspase-3 was determined by Western blot (WB); and nuclear transposition of apoptosis-inducing factor (AIF) was tested by immunocytochemistry and WB. By MitoSOX staining, the mitochondrial ROS were detected. Mitochondrial function was also tested by MTT assay (content of succinic dehydrogenase), flow cytometry (mitochondrial membrane potential (MMP)-JC-1 staining; mitochondrial abundance-mito-Tracker green), immunofluorescence (MMP-JC-1 staining; mitochondrial morphology-mito-Tracker green), WB (mitochondrial fusion-fission protein-OPA1, mfn2, and DRP1; mitophagy-related proteins-Pink1, Parkin, LC3B, and P62), and high-performance liquid chromatography (HPLC) (energy charge). Finally, mitochondrial protein homeostasis of C6 cells after XN treatment with and without LONP1 inhibitor bortezomib was investigated by trypan blue assay (proliferative activity and mortality) and WB (mitochondrial protease LONP1). All cell morphology images were taken by a Leica Microsystems microscope.

Results: XN could lead to proliferation inhibition and death of C6 cells in a time- and dose-dependent manner and induce apoptosis of C6 cells through the AIF pathway. After long incubation of XN, mitochondria of C6 cells were seriously impaired, and mitochondria had a diffuse morphology and mitochondrial ROS were increased. The content of succinic dehydrogenase per cell was significantly decreased after XN insults of 24, 48, and 72 h. The energy charge was weakened after XN insult of 24 h. Furthermore, the MMP and mitochondrial abundance were significantly decreased; the protein expression levels of OPA1, mfn2, and DRP1 were down-regulated; and the protein expression levels of Pink1, Parkin, LC3B-II/LC3B-I, and p62 were up-regulated in long XN incubation times (24, 48, and 72 h). XN incubation with bortezomib for 48 h resulted in lower proliferative activity and higher mortality of C6 cells and caused the cell to have visible vacuoles. Moreover, the protein expression levels of LONP1 was up-regulated gradually as XN treatment time increased.

Conclusion: These data supported that XN could induce AIF pathway apoptosis of the rat glioma C6 cells by affecting the mitochondria.
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http://dx.doi.org/10.3390/ijms22094506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123451PMC
April 2021

Achieving Highly Efficient Carbon Dioxide Electrolysis by Construction of the Heterostructure.

ACS Appl Mater Interfaces 2021 May 22;13(17):20060-20069. Epub 2021 Apr 22.

Beijing Key Laboratory for Chemical Power Source and Green Catalysis, Beijing Institute of Technology, Beijing 100081, People's Republic of China.

The design of active cathode catalysts, with abundant active sites and outstanding catalytic activity for CO electroreduction, is important to promote the development of solid oxide electrolysis cells (SOECs). Herein, A-site-deficient perovskite oxide (LaSr)TiMnCuO (LSTMC) is synthesized and studied as a promising cathode for SOECs. Cu nanoparticles can be rapidly and uniformly -exsolved under reducing conditions. The heterostructure formed by the exsoluted Cu and LSTMC provides abundant active sites for the catalytic conversion of CO to CO. Combined with the remarkable oxygen-ion transport capacity of the LSTMC substrate, the specially designed [email protected] cathode exhibits a dramatically improved electrochemical performance. Furthermore, first-principles calculations proposed a mechanism for the adsorption and activation of CO by the heterostructure. Electrochemically, the [email protected] presents a high current density of 2.82 A cm at 1.8 V and 800 °C, which is about 2.5 times higher than that of LSTM (1.09A cm).
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http://dx.doi.org/10.1021/acsami.1c02146DOI Listing
May 2021

Pr-Doping Motivating the Phase Transformation of the BaFeO- Perovskite as a High-Performance Solid Oxide Fuel Cell Cathode.

ACS Appl Mater Interfaces 2021 Apr 22. Epub 2021 Apr 22.

Beijing Key Laboratory for Chemical Power Source and Green Catalysis, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, People's Republic of China.

Intermediate temperature solid oxide fuel cells (IT-SOFCs) have been extensively studied due to high efficiency, cleanliness, and fuel flexibility. To develop highly active and stable IT-SOFCs for the practical application, preparing an efficient cathode is necessary to address the challenges such as poor catalytic activity and CO poisoning. Herein, an efficient optimized strategy for designing a high-performance cathode is demonstrated. By motivating the phase transformation of BaFeO perovskites, achieved by doping Pr at the B site, remarkably enhanced electrochemical activity and CO resistance are thus achieved. The appropriate content of Pr substitution at Fe sites increases the oxygen vacancy concentration of the material, promotes the reaction on the oxygen electrode, and shows excellent electrochemical performance and efficient catalytic activity. The improved reaction kinetics of the BaFePrO (BFP05) cathode is also reflected by a lower electrochemical impedance value (0.061 Ω·cm at 750 °C) and activation energy, which is attributed to high surface oxygen exchange and chemical bulk diffusion. The single cells with the BFP05 cathode achieve a peak power density of 798.7 mW·cm at 750 °C and a stability over 50 h with no observed performance degradation in CO-containing gas. In conclusion, these results represent a promising optimized strategy in developing electrode materials of IT-SOFCs.
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http://dx.doi.org/10.1021/acsami.1c03514DOI Listing
April 2021

One-pot synthesis of mesoporous palladium/C nanodendrites as high-performance oxygen reduction eletrocatalysts through a facile dual surface protecting agent-assisted strategy.

Dalton Trans 2021 May;50(18):6297-6305

Department of Materials Science and Engineering, Jiangsu Key Laboratory of Artificial Functional Materials, Collaborative Innovation Center of Advanced Microstructures, College of Engineering and Applied Sciences, Nanjing University, Nanjing 210023, P. R. China.

Palladium (Pd) is regarded as a potential non-platinum electrocatalyst to drive oxygen reduction in fuel cells. The development of Pd-based electrocatalysts with high performances through structural engineering is still highly desirable. Herein, a facile one-pot synthesis strategy with the assistance of dual surface protecting agents was developed to fabricate carbon-supported Pd (Pd/C) nanodendrites with high mesoporosity. The mesoporous spherical Pd/C nanodendrites are built with connected nanoparticles with a small size of several nanometers and coated by simultaneously formed carbon layers. The used dual protecting agents, glycine and oleylamine, exhibit synergistic effects to engineer Pd growth to form the unique mesoporous dendritic structure. Benefiting from the mesoporous feature, small size, defect-rich surface and carbon coating, the obtained mesoporous Pd/C nanodendrites exhibit great electrocatalytic performance toward the oxygen reduction reaction (ORR).
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http://dx.doi.org/10.1039/d1dt00026hDOI Listing
May 2021

Maize Is Involved in Drought Resistance and Recovery Ability Through an Abscisic Acid-Dependent Signaling Pathway.

Front Plant Sci 2021 1;12:629903. Epub 2021 Apr 1.

Grain Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, China.

Analyzing the transcriptome of maize leaves under drought stress and rewatering conditions revealed that transcription factors were involved in this process, among which of the ABSCISIC ACID-INSENSITIVE 5-like protein 5 family was induced to significantly up-regulated. The functional mechanism of in Abscisic acd (ABA) signaling pathway and its response to drought stress and rewatering has not been studied yet. The present study found that ZmbZIP33 contains a DNA-binding and dimerization domain, has transcriptional activation activity, and is highly homologous to SbABI1,SitbZIP68 and OsABA1. The expression of is strongly up-regulated by drought, high salt, high temperature, and ABA treatments. Overexpression of remarkably increased chlorophyll content and root length after drought stress and rewatering, and, moreover, cause an accumulation of ABA content, thereby improving drought resistance and recovery ability in . However, silencing the expression of (BMV-ZmbZIP33) remarkably decreased chlorophyll content, ABA content, superoxide dismutase and peroxidase activities, and increased stomatal opening and water loss rate compared with BMV (control). It showed that silencing lead to reduced drought resistance and recovery ability of maize. ABA sensitivity analysis found that 0.5 and 1 μmol/L treatments severely inhibited the root development of overexpression transgenic . However, the root growth of BMV was greatly inhibited for 1 and 5μmol/L ABA treatments, but not for BMV-ZmbZIP33. Subcellular localization, yeast two-hybrid and BIFC further confirmed that the core components of ABA signaling pathways ZmPYL10 and ZmPP2C7 interacted in nucleus, ZmPP2C7 and ZmSRK2E as well as ZmSRK2E and ZmbZIP33 interacted in the plasma membrane. We also found that expression levels of and in the BMV-ZmbZIP33 mutant were lower than those of BMV, while was the opposite under drought stress and rewatering. However, expression of and in normal maize leaves were significantly up-regulated by 3-4 folds after drought and ABA treatments for 24 h, while was down-regulated. The and encoding key enzymes in ABA biosynthesis are up-regulated in overexpression transgenic line under drought stress and rewatering conditions, but down-regulated in BMV-ZmbZIP33 mutants. Together, these findings demonstrate that played roles in ABA biosynthesis and regulation of drought response and rewatering in and maize thought an ABA-dependent signaling pathway.
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http://dx.doi.org/10.3389/fpls.2021.629903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048716PMC
April 2021

Layer-specific strain and dyssynchrony index alteration in new-onset systemic lupus erythematosus patients without cardiac symptoms.

Quant Imaging Med Surg 2021 Apr;11(4):1271-1283

Department of Rheumatology, Second Affiliated Hospital of Fujian Medical University, Quanzhou, China.

Background: Layer-specific speckle-tracking echocardiography (STE) is a noninvasive approach that assesses subclinical left ventricular dysfunction. We aimed to investigate the (I) alteration of layer-specific STE parameters and the dyssynchrony index; and (II) the disease parameters associated with layer-specific STE change in drug-naïve patients with new-onset systemic lupus erythematosus (SLE) without cardiac symptoms.

Methods: Thirty-five drug-naïve patients with new-onset SLE and twenty-five healthy controls were enrolled. All individuals received both conventional echocardiographic and two-dimensional STE assessment. The data of layer-specific global longitudinal strain (GLS), global circumferential strain (GCS), and peak systolic dispersion (PSD) were acquired in layer-specific STE.

Results: All patients had a normal left ventricular ejection fraction (LVEF)(mean LVEF: 58%) and conventional echocardiographic parameters were comparable between patients and controls. Decreased layer-specific GLS and elevated PSD were observed in SLE patients (whole layer GLS: -17.6%±3.0% versus -19.3%±2.6%, P=0.02; endocardial GLS: -20.0%±3.2% versus -22.1%±3.0%, P=0.01; epicardial GLS: -15.6%±2.7% versus -16.8%±2.4%, P=0.04; PSD: 41.0±18.9 versus 28.8±10.1 msec, P=0.007). In contrast, there was no difference in layer-specific GCS at three different levels between patients and controls (P>0.05). More severely impaired GLS was observed in patients with higher disease activity, high-risk antiphospholipid antibody (aPL) profile, or renal involvement. The PSD was increased in patients with higher disease activity or a high-risk aPL profile. Correlational analysis showed that GLS at three layers and PSD correlated with high-sensitivity C-reactive protein (hsCRP) levels (whole GLS: r=0.662, P<0.001; endocardial GLS: r=0.637, P<0.001; epicardial GLS: r=0.658, P<0.001; PSD: r=0.390, P=0.021). PSD correlated with epicardial GLS (r=0.360, P=0.047), when treating the hsCRP level, renal involvement, aPL profile, and disease activity as control variables. Multivariate regression showed the hsCRP level and epicardial GLS were predictors of layer-specific GLS impairment and elevated PSD, respectively.

Conclusions: Drug-naive patients with new-onset SLE are likely to have subclinical GLS impairment and left ventricular dyssynchrony, even in the presence of normal LVEF. SLE-related risk factors are associated with these dysfunctions.
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http://dx.doi.org/10.21037/qims-20-859DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930686PMC
April 2021

Energy dissipation efficiency as a new variable in the empirical correlation of total dissolved gas.

Sci Rep 2021 Apr 1;11(1):7414. Epub 2021 Apr 1.

China Three Gorges Projects Development Co., Ltd, Chengdu, China.

Total dissolved gas (TDG) supersaturation, which occurs during dam spilling, may result in fish bubble disease and mortality. Many studies have been conducted to identify the factors pertaining to TDG generation, such as the spilling discharge and tailwater depth. Additionally, the energy dissipation efficiency should be considered due to its effect on the air entrainment, which influences the TDG generation process. According to the TDG field observations of 49 cases at Dagangshan and Xiluodu hydropower stations, the TDG was positively related to the energy dissipation efficiency, tailwater depth and discharge per unit width. A correlation between the generated TDG level and these factors was established. The empirical equations proposed by the USACE were calibrated, and the TDG level estimation performance was compared with the established correlation for 25 spillage cases at seven other dams. Among the considered cases, the standard error of the TDG estimation considering the energy dissipation efficiency was 5.7%, and those for the correlations obtained using the USACE equations were 13.0% and 10.0%. The findings indicated that the energy dissipation efficiency considerably influenced the TDG level, and its consideration helped enhance the precision of the TDG estimation. Finally, the generality of this approach and future work were discussed.
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http://dx.doi.org/10.1038/s41598-021-86144-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016975PMC
April 2021

Occurrence of macroprolactinemia in schizophrenia patients treated with risperidone or amisulpride.

Asia Pac Psychiatry 2021 Mar 22:e12469. Epub 2021 Mar 22.

Huzhou Third Municipal Hospital, the Affiliated Hospital of Huzhou University, Zhejiang, China.

Objective: To investigate macroprolactinemia caused by antipsychotics and its clinical significance.

Methods: A total of 133 patients with schizophrenia were selected, all of whom were treated with either risperidone or amisulpride alone. The levels of total prolactin (T-PRL) and macroprolactin (MPRL) were measured before treatment as well as the second, fourth, and sixth weeks of treatment.

Results: After 2 weeks of treatment, 75.09% (100/133) of the patients met the diagnostic criteria for hyperprolactinemia, the incidence of macroprolactinemia was 43% (43/100), and MPRL levels were positively correlated T-PRL levels.

Conclusion: Risperidone and amisulpride caused hyperprolactinemia and macroprolactinemia; thus, detection of MPRL in the clinical setting should be performed as this phenomenon appears early in treatment (the second week) and continues, that can avoid unnecessary examination and treatment for asymptomatic patients with macroprolactinemia.
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http://dx.doi.org/10.1111/appy.12469DOI Listing
March 2021

Characterization, antioxidant, and neuroprotective effects of anthocyanins from Nitraria tangutorum Bobr. fruit.

Food Chem 2021 Aug 3;353:129435. Epub 2021 Mar 3.

CAS Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Xining 810008, PR China; Huzhou Plateau Biological Resource Centre of Innovation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Huzhou 313000, PR China; Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining 810001, PR China. Electronic address:

An anthocyanin-rich extract was obtained from Nitraria tangutorum Bobr. fruit, namely ANF, and its composition, antioxidant and neuroprotective effects were studied. Nine anthocyanins were identified from the ANF using UPLC-Triple-TOF/MS analysis, and cyanidin-3-[2''-(6'''-coumaroyl)-glucosyl]-glucoside (C3G) is the most abundant anthocyanin (87.06%). ANF exhibited high ferric reducing antioxidant power (FRAP) and ABTS radical scavenging activity. The online HPLC-DPPH screening revealed that C3G contributed the highest antioxidant capacity. ANF showed potential neuroprotective effects by relieving d-Galactose-induced memory deficits, reducing overexpression of receptor for advanced glycation end products (RAGE) and amyloid-beta42 (Aβ42) in the hippocampus of rats. Besides, ANF could inhibit oxidative stress by reducing the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) in the hippocampus, while elevating amounts of total superoxide dismutase (T-SOD) and glutathione (GSH) in the serum of rats. Thus, ANF has great potential in the development of food and health products related to antioxidant and neuroprotective effects.
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http://dx.doi.org/10.1016/j.foodchem.2021.129435DOI Listing
August 2021

A Joint Model for Macular Edema Analysis in Optical Coherence Tomography Images Based on Image Enhancement and Segmentation.

Biomed Res Int 2021 17;2021:6679556. Epub 2021 Feb 17.

College of Information Science, Shanghai Ocean University, Shanghai 201306, China.

Optical coherence tomography (OCT) provides the visualization of macular edema which can assist ophthalmologists in the diagnosis of ocular diseases. Macular edema is a major cause of vision loss in patients with retinal vein occlusion (RVO). However, manual delineation of macular edema is a laborious and time-consuming task. This study proposes a joint model for automatic delineation of macular edema in OCT images. This model consists of two steps: image enhancement using a bioinspired algorithm and macular edema segmentation using a Gaussian-filtering regularized level set (SBGFRLS) algorithm. We then evaluated the delineation efficiency using the following parameters: accuracy, precision, sensitivity, specificity, Dice's similarity coefficient, IOU, and kappa coefficient. Compared with the traditional level set algorithms, including C-V and GAC, the proposed model had higher efficiency in macular edema delineation as shown by reduced processing time and iteration times. Moreover, the accuracy, precision, sensitivity, specificity, Dice's similarity coefficient, IOU, and kappa coefficient for macular edema delineation could reach 99.7%, 97.8%, 96.0%, 99.0%, 96.9%, 94.0%, and 96.8%, respectively. More importantly, the proposed model had comparable precision but shorter processing time compared with manual delineation. Collectively, this study provides a novel model for the delineation of macular edema in OCT images, which can assist the ophthalmologists for the screening and diagnosis of retinal diseases.
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http://dx.doi.org/10.1155/2021/6679556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904365PMC
May 2021

CCMAlnc Promotes the Malignance of Colorectal Cancer by Modulating the Interaction Between miR-5001-5p and Its Target mRNA.

Front Cell Dev Biol 2020 16;8:566932. Epub 2020 Dec 16.

State Key Laboratory of Oncogenes and Related Genes, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Objective: Colorectal cancer (CRC) is highly malignant and cancer metastasis remains the predominant cause of CRC death. The potential molecular mechanism of long non-coding RNA (lncRNAs) in CRC malignance is still poorly elucidated.

Methods: CCMAlnc expression was analyzed by using the Sequence ReadArchive (SRA) database. Target gene expression was examined by real-time PCR and Western blotting. The biological function of CCMAlnc and miR-5001-5p was detected by cell invasion, CCK8 proliferation, and colony formation assays in loss of function and gain of function experiments . A luciferase assay was performed to validate the target site of miR-5001-5p on the 3'-UTR of HES6 mRNA.

Results: CCMAlnc was identified as a novel functional lncRNA in CRC. Elevated CCMAlnc was detected in CRC cells as well as in clinical CRC tissue samples, and the expression of this lncRNA positively correlated with the poor prognosis of CRC patients. Functional validation assays revealed that downregulation of CCMAlnc impaired CRC cell proliferation and invasion , but upregulation of CCMAlnc reversed this effect. Moreover, CCMAlnc was validated to act as a competing endogenous RNA (ceRNA) that stabilizes the expression of HES6 by downregulating miR-5001-5p.

Conclusion: CCMAlnc/miR-5001-5p/HES6 signaling is strongly activated to promote CRC malignance. CCMAlnc is defined as a potential candidate biomarker for metastasis prediction in CRC patients and as a potential therapeutic target for CRC treatment.
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http://dx.doi.org/10.3389/fcell.2020.566932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931267PMC
December 2020

Ultrastable FeCo Bifunctional Electrocatalyst on Se-Doped CNTs for Liquid and Flexible All-Solid-State Rechargeable Zn-Air Batteries.

Nano Lett 2021 Mar 18;21(5):2255-2264. Epub 2021 Feb 18.

Frontiers Science Center for Flexible Electronics, Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials and Engineering, Northwestern Polytechnical University, Xi'an 710072, China.

The rechargeable Zn-air batteries as an environmentally friendly sustainable energy technology have been extensively studied. However, it is still a challenge to develop non-noble metal bifunctional catalysts with high oxygen reduction as well as oxygen evolution reaction (ORR and OER) activity and superior durability, which limit the large-scale application of rechargeable Zn-air batteries. Herein, we synthesized an ultrastable FeCo bifunctional oxygen electrocatalyst on Se-doped CNTs (FeCo/Se-CNT) via a gravity guided chemical vapor deposition (CVD) strategy. The catalyst exhibits excellent ORR ( = 0.9 V) and OER (overpotential at 10 mA cm = 340 mV) properties simultaneously, surpassing commercial Pt/C and RuO/C catalysts. More importantly, the catalyst shows an unordinary stability, that is, is no obvious decrease after 30K cycles accelerated durability test for ORR and OER processes. The small potential gap (0.75 V) represents superior bifunctional ORR and OER activities of the FeCo/Se-CNT catalyst. The FeCo/Se-CNT catalyst possesses outstanding electrochemical performance for the rechargeable liquid and flexible all-solid-state Zn-air batteries, for example, a high open circuit voltage (OCV) and peak power density of 1.543 and 1.405 V and 173.4 and 37.5 mW cm, respectively.
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http://dx.doi.org/10.1021/acs.nanolett.1c00077DOI Listing
March 2021

Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6ρ0 cells.

Mol Med Rep 2021 04 12;23(4). Epub 2021 Feb 12.

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, P.R. China.

Malignant tumors of the central nervous system (CNS) are among the types of cancer with the poorest prognosis and glioma is the commonest primary CNS tumor. A mitochondrial DNA (mtDNA)‑depleted cell line C6ρ0 was generated from C6 glioma cells after long‑term exposure to ethidium bromide and 2',3'‑dideoxycytidine in order to determine the effect of mtDNA damage on cell proliferation and pathological changes in glioma cells. Single cell clones were isolated and identified after 42 days of incubation. Repopulated cybrids were formed when the clonal C6ρ0 cells were fused with rat platelets and no difference was observed in their growth in a selective medium without uridine and pyruvate compared with the growth of the parent C6 cells. Disruption of mtDNA resulted in changes in mitochondrial morphology, decreased cell proliferation, reduced intracellular reactive oxygen species and intracellular ATP, along with decreased mtDNA and mitochondrial membrane potential in C6ρ0 cells compared with the C6 cells. Taken together, C6ρ0 cells without mtDNA were established for the first time and their characteristics were compared with parent cells. This C6ρ0 cell line could be used to explore the contribution of mitochondrial dysfunction and mtDNA mutations in the pathogenesis of glioma.
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http://dx.doi.org/10.3892/mmr.2021.11904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893707PMC
April 2021

Genome-Wide Characterization of Jasmonates Signaling Components Reveals the Essential Role of ZmCOI1a-ZmJAZ15 Action Module in Regulating Maize Immunity to Gibberella Stalk Rot.

Int J Mol Sci 2021 Jan 16;22(2). Epub 2021 Jan 16.

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.

Gibberella stalk rot (GSR) by causes significant losses of maize production worldwide. Jasmonates (JAs) have been broadly known in regulating defense against pathogens through the homeostasis of active JAs and COI-JAZ-MYC function module. However, the functions of different molecular species of JAs and COI-JAZ-MYC module in maize interactions with and regulation of diverse metabolites remain unknown. In this study, we found that exogenous application of MeJA strongly enhanced resistance to GSR. RNA-seq analysis showed that MeJA activated multiple genes in JA pathways, which prompted us to perform a genome-wide screening of key JA signaling components in maize. Yeast Two-Hybrid, Split-Luciferase, and Pull-down assays revealed that the JA functional and structural mimic coronatine (COR) functions as an essential ligand to trigger the interaction between ZmCOIa and ZmJAZ15. By deploying CRISPR-cas9 knockout and insertional mutants, we demonstrated that mutant is more resistant, whereas mutant is more susceptible to GSR. Moreover, JA-deficient mutant displayed enhanced resistance to GSR compared to wild type. Together, these results provide strong evidence that plays a pivotal role, whereas and endogenous JA itself might function as susceptibility factors, in maize immunity to GSR.
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http://dx.doi.org/10.3390/ijms22020870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830991PMC
January 2021

Enhancing Polysulfide Confinement and Electrochemical Kinetics by Amorphous Cobalt Phosphide for Highly Efficient Lithium-Sulfur Batteries.

ACS Nano 2021 Jan 28;15(1):739-750. Epub 2020 Dec 28.

Beijing Key Laboratory for Chemical Power Source and Green Catalysis, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, P.R. China.

The application of lithium-sulfur (Li-S) batteries is severely hampered by the shuttle effect and sluggish redox kinetics. Herein, amorphous cobalt phosphide grown on a reduced graphene oxide-multiwalled carbon nanotube (rGO-CNT-CoP(A)) is designed as the sulfur host to conquer the above bottlenecks. The differences between amorphous cobalt phosphide (CoP) and crystalline CoP on the surface adsorption as well as conversion of lithium polysulfides (LiPSs) are investigated by systematical experiments and density-functional theory (DFT) calculations. Specifically, the amorphous CoP not only strengthens the chemical adsorption to LiPSs but also greatly accelerates liquid-phase conversions of LiPSs as well as the nucleation and growth of LiS. DFT calculation reveals that the amorphous CoP possesses higher binding energies and lower diffusion energy barriers for LiPSs. In addition, the amorphous CoP features reduced energy gap and the increased electronic concentrations of adsorbed LiPSs near Fermi level. These characteristics contribute to the enhanced chemisorption ability and the accelerated redox kinetics. Simultaneously, the prepared S/rGO-CNT-CoP(A) electrode delivers an impressive initial capacity of 872 mAh g at 2 C and 617 mAh g can be obtained after 200 cycles, exhibiting excellent cycling stability. Especially, it achieves outstanding electrochemical performance even under high sulfur loading (5.3 mg cm) and lean electrolyte (/ = 7 μL mg) conditions. This work exploits the application potential for amorphous materials and contributes to the development of highly efficient Li-S batteries.
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http://dx.doi.org/10.1021/acsnano.0c07038DOI Listing
January 2021

A New Information-Theoretic Method for Advertisement Conversion Rate Prediction for Large-Scale Sparse Data Based on Deep Learning.

Entropy (Basel) 2020 Jun 10;22(6). Epub 2020 Jun 10.

Beijing Insititute of Control Engineering, Beijing 100191, China.

With the development of online advertising technology, the accurate targeted advertising based on user preferences is obviously more suitable both for the market and users. The amount of conversion can be properly increased by predicting the user's purchasing intention based on the advertising Conversion Rate (CVR). According to the high-dimensional and sparse characteristics of the historical behavior sequences, this paper proposes a LSLM_LSTM model, which is for the advertising CVR prediction based on large-scale sparse data. This model aims at minimizing the loss, utilizing the Adaptive Moment Estimation (Adam) optimization algorithm to mine the nonlinear patterns hidden in the data automatically. Through the experimental comparison with a variety of typical CVR prediction models, it is found that the proposed LSLM_LSTM model can utilize the time series characteristics of user behavior sequences more effectively, as well as mine the potential relationship hidden in the features, which brings higher accuracy and trains faster compared to those with consideration of only low or high order features.
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http://dx.doi.org/10.3390/e22060643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7517176PMC
June 2020

Clinical Observation and Prognostic Analysis of Patients With Bloodstream Infection.

Front Cell Infect Microbiol 2020 9;10:577244. Epub 2020 Nov 9.

Department of General Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou Key Laboratory of Sepsis, Henan Key Laboratory of Critical Care Medicine, Zhengzhou, China.

Background And Purpose: The clinical prognosis of Klebsiella pneumoniae() bloodstream infection is poor, and the prevalence of drug-resistant bacteria makes clinical anti-infective treatment more challenging. This retrospective study evaluated the epidemiological characteristics of patients with , the risk factors for drug-resistant bacterial infection and death, and analyzed treatment options.

Methods: Clinical data of 297 patients diagnosed with bacteremia between June 2014 and June 2019 were collected.

Results: Intensive care unit hospitalization history, operation history, recent antibiotic use history, mechanical ventilation, and number of days hospitalized before bloodstream infection were found to be independent risk factors for drug-resistant bacterial infection. The risk of death for carbapenem-resistant infection was 2.942 times higher than that for carbapenem-sensitive infection. For extensively drug-resistant bacteremia patients, the mortality rate of combined anti-infective therapy was lower.

Conclusions: Clinicians should pay attention to patients with high-risk drug-resistant bacteria infection and administer timely anti-infection treatment. The findings of this study may provide some suggestions for early identification and standardized treatment of patients with bacteremia.
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http://dx.doi.org/10.3389/fcimb.2020.577244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680902PMC
November 2020

Magnetic solid-phase extraction for speciation of mercury based on thiol and thioether-functionalized magnetic covalent organic frameworks nanocomposite synthesized at room temperature.

J Chromatogr A 2021 Jan 13;1635:461712. Epub 2020 Nov 13.

Shandong Analysis and Tester Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.; Shandong Key Laboratory for Adhesive Materials, Advanced Materials Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.. Electronic address:

A simple and practical magnetic solid-phase extraction high-performance liquid chromatography-inductively coupled plasma mass spectrometry (MSPE-HPLC-ICP-MS) method for extraction and determination of trace mercury species, including inorganic mercury (IHg), monomethylmercury (MeHg) and ethylmercury (EtHg), was developed. The MSPE adsorbent, urchin-like thiol and thioether-functionalized magnetic covalent organic frameworks ([email protected]), was synthesized by coating covalent organic frameworks (COFs) on the surface of FeO nanoparticles at room temperature and then easily grafting 1,2-Ethanedithiol on the COFs. The as-prepared [email protected] has strong adsorption capacity for IHg, MeHg and EtHg, with excellent static adsorption capacity: 571, 559 and 564 mg g, respectively. The parameters influencing the extraction and enrichment had been optimized, including pH, adsorption and desorption time, composition and amount of the eluent, co-existing ions and dissolved organic materials etc. Under the optimized condition, the limit of detection (3δ) of the proposed method were 0.96, 0.17 and 0.47 ng L for IHg, MeHg and EtHg, and the developed method has high actual enrichment factors of 370, 395, 365-fold for IHg, MeHg and EtHg based on 200 mL samples, respectively. The high accuracy and reproducibility has been proved by the spiked recoveries (96.0‒108 %) in real water samples and determination of the certified reference material. Both the adsorption and desorption process can be completed within 5 min. The proposed method with simple operation, short pre-concentration time and high sensitivity has been successfully applied to mercury speciation at trace levels in the samples with complicated matrices, including underground water, surface water, sea water and fish samples.
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http://dx.doi.org/10.1016/j.chroma.2020.461712DOI Listing
January 2021

Recombinant osteopontin provides protection for cerebral infarction by inhibiting the NLRP3 inflammasome in microglia.

Brain Res 2021 01 4;1751:147170. Epub 2020 Nov 4.

Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710000, China. Electronic address:

Neuroinflammation is one of the most important secondary pathological events after cerebral infarction. Activation of NLRP3 inflammasome is a pivotal form of neuroinflammation. Osteopontin (OPN) is expressed during the subacute phase after cerebral infarction and has an important chemotactic effect on microglia. The aim of this study was to reveal the effect of recombinant OPN on brain injury after cerebral infarction and the regulation of NLRP3 inflammasome. We used the middle cerebral artery occlusion (MCAO) method-established focal cerebral ischemia model and LPS-induced inflammation model on neonate rat primary microglia. The effects of OPN on cerebral ischemic injury, neural function, microglia inflammation and NLRP3 inflammasome function were studied by immunofluorescence, staining, enzyme-linked immunosorbent assay and Western blot assay. We established MCAO cerebral ischemia and reperfusion injury model, and found that recombinant OPN reduced the volume of cerebral infarction and alleviated the ischemic injury degree of cerebral tissues, neurons, and neurological function. We found that OPN was also involved in the negative regulation of inflammasome and microglia activity in cerebral ischemic injury, and that OPN inhibited the activation of NLRP3 inflammasome and the function of microglia in a LPS-induced inflammatory model. Our findings show that recombinant OPN can reduce the ischemic infarct size and alleviate the cerebral ischemic injury of rats, which may be related to its efficient involvement in the inhibitory regulation of inflammasome and microglia inflammatory activation.
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http://dx.doi.org/10.1016/j.brainres.2020.147170DOI Listing
January 2021

Oligostilbenes extracts from Iris lactea Pall. var. chinensis (Fisch.) Koidz improve lipid metabolism in HFD/STZ-induced diabetic mice and inhibit adipogenesis in 3T3-L1 cells.

Biomed Pharmacother 2020 Nov 29;131:110800. Epub 2020 Sep 29.

Key Laboratory of Tibetan Medicine Research, Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810008, PR China. Electronic address:

The present study investigated the anti-diabetic effects of Oligostilbenes extracts (Olie) from Iris lactea Pall. var. chinensis (Fisch.) Koidz (I. lactea) and the potential mechanisms, in high-fat-diet (HFD)-induced diabetic mice and 3T3-L1 adipocytes. Olie are a group of major active extracts from I. lactea that have been used as nutraceutical because of their antioxidant activity. Six-week Olie treatment improved fasting blood glucose levels, as well as blood lipid profiles in HFD/streptozocin (STZ)-induced diabetic mice, compared with non-treated mice. Olie treatment upregulated the levels of phosphorylated of AMPK and lipolysis-related proteins, while the hepatic expression of ACC and FAS in diabetic mice was inhibited. In cultured 3T3-L1 cells, Olie (2-15 μg/mL) treatment dose-dependently suppressed the differentiation into mature adipocytes and lowered cellular lipid accumulation. Consistently, Olie reduced expression of adipogenic transcription factors including CCAAT/enhancer-binding protein β (C/EBPβ) and peroxisome proliferator-activated receptor γ (PPARγ). In addition, mitochondrial function in 3T3-L1 adipocytes was improved after Olie treatment. Taken together, our findings indicate that a lipid-lowering effect of Olie in HFD/STZ-induced diabetic mice and adipogenesis/ lipogenesis suppressing effect in 3T3-L1 cells, via regulating the expression of lipid metabolism-related proteins.
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http://dx.doi.org/10.1016/j.biopha.2020.110800DOI Listing
November 2020

Chronic REM-sleep deprivation induced laryngopharyngeal reflux in rats: A preliminary study.

Auris Nasus Larynx 2021 Aug 2;48(4):683-689. Epub 2020 Nov 2.

Department of Otolaryngology, the 8th Medical Center of Chinese PLA General Hospital, Beijing 100091, China; College of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing 100048, China; National Clinical Research Center for Otolaryngology Diseases, Beijing 100853, China.

Objective: The aim of this study was to investigate the relationship of chronic REM-sleep deprivation with laryngopharyngeal reflux (LPR) and its mechanism.

Methods: Forty healthy male SD rats (body weight 250-280 g) were randomly divided into four groups. The first three ones were test group, which underwent REM-sleep deprivation with different duration of time by modified multiplatform water surface method. The last group was the control one having normal sleep. All the animals were performed Dx-pH monitoring when finishing sleep deprivation, and sacrificed to study the gastric residual rate (GRR) and small intestine peristalsis (SPR) rate by charcoal meal method.

Results: At prone position, the reflux incidence in the test groups fairly increased with the duration of sleep deprivation (p<0.05). The total number of reflux episodes at prone position in the test group rats with 3 months duration of sleep deprivation was significantly increased compared with that in the control ones (p<0.05). GRR in rats experiencing sleep deficiency for different duration all reduced significantly when compared to the control group (p<0.05). GRR and SPR presented continuous decline tendency with the duration of sleep deprivation (p>0.05).

Conclusions: It is suggested that chronic sleep deficiency could cause LPR in rats, which might result from the uncoordinated digestive tract motility caused by dysfunction of central nervous system after chronic REM-sleep deprivation. Our results implied that chronic REM-sleep deprivation might be one of the causes of LPR. Addressing sleep problems might help to decrease the prevalence of LPR and enhance its treatment efficacy.
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http://dx.doi.org/10.1016/j.anl.2020.10.011DOI Listing
August 2021

Solidification/stabilization of heavy metals and its efficiency in lead-zinc tailings using different chemical agents.

Environ Technol 2020 Nov 21:1-11. Epub 2020 Nov 21.

School of Materials Science and Engineering, Zhengzhou University, Zhengzhou, People's Republic of China.

Lead-zinc tailings are generated during the mining process which is considered as hazardous solid waste due to its high heavy metal content and leachability in the natural state. At present, the most effective technology for disposing heavy metals in solid wastes is the solidification/stabilization (S/S) technique. In terms of S/S technology, chemical stabilization is one of the most potential and practical method. This paper aims to investigate the S/S property of four typical chemical agents (NaS, NaHPO, TMT and NaEDTA) on the heavy metals in lead-zinc tailings. The results reveal that the heavy metals lead and zinc in tailings are stabilized more effectively by using chelating agents TMT than by using inorganic chemical agents NaS and NaHPO. When the dosage of TMT reaches 4%, the leaching concentration of lead and zinc is 0.18 and 14.60 mg/L according to toxicity characteristic leaching procedure (TCLP), and the stabilization efficiency of lead and zinc is 99.31% and 80.92%, respectively, while the leaching concentration of lead and zinc just drops to 0.41 and 16.00 mg/L with addition of 10% NaHPO. Furthermore, the leaching concentration of heavy metal lead in tailings treated by 4% NaEDTA increases to 53.44 mg/L which far exceeds the standard of pollution control. Therefore, considering stabilization efficiency and dosage, TMT is the preferred agent for solidifying heavy metals in lead-zinc tailings.
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http://dx.doi.org/10.1080/09593330.2020.1845817DOI Listing
November 2020

Canopy processing of N deposition increases short-term leaf N uptake and photosynthesis, but not long-term N retention for aspen seedlings.

New Phytol 2021 03 3;229(5):2601-2610. Epub 2020 Dec 3.

State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

Forest canopies can retain nitrogen (N) from atmospheric deposition. However, most empirical and modeling studies do not consider the processing of the N deposited in the canopy. To assess whether N deposition through canopy will alter the plant's N uptake and retention, we conducted a 3-yr mesocosm experiment by applying ( NH ) SO solution to aspen sapling canopies or directly to the soil. We found that N-NH applied to the canopy was directly taken up by leaves. Compared with the soil N application, the canopy N application resulted in higher photosynthesis but lower N retention of the plant-soil system in the first growing season. Plant biomass, N concentration, and leaf N resorption were not significantly different between the canopy and soil N applications. The partitioning of retained N among plant components and soil layers was similar between the two treatments 3 yr after the N application. Our findings indicated that the canopy N processing could alter leaf N supply and photosynthesis in the short term but not N retention in the long term. Under natural conditions, the chronic N deposition could continuously refill the canopy N pool, causing a sustained increase in canopy carbon uptake. Canopy N processing needs to be considered for accurately predicting the impact of N deposition.
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http://dx.doi.org/10.1111/nph.17041DOI Listing
March 2021

Design and analysis of full-scale scanning system for curved glass based on motion and 3D features.

Appl Opt 2020 Oct;59(29):9195-9205

In recent years, mobile phones with glass curved screens have become more and more widely used. The irregular shape of the curved screen and the light transmittance characteristic of the glass have brought great challenges to its automatic defect detection. Aiming at the defect detection of the glass cover of the curved screen, this paper designs a full-scale scanning system by combining motion and three-dimensional (3D) features. First, a scanning system is constructed, and a geometric error modeling method is proposed to improve the accuracy of the scanning system; second, based on the point cloud of the 3D glass cover obtained by the scanning system, a point cloud registration method is presented by integrating the motion and 3D features; finally, the laser tracker is further used to calibrate the scanning system to analyze the mechanical error. Experimental results show that the introduction of straightness error and perpendicularity error can effectively solve the mismatch and fault problems of point cloud registration, and improve the accuracy of the scanning system. In addition, the registration method proposed in this paper can effectively reconstruct the complete point cloud of 3D glass cover for detection. The reconstruction accuracy of the plane part can reach 0.031 mm, and that of the curved part can reach 0.091 mm.
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http://dx.doi.org/10.1364/AO.403433DOI Listing
October 2020

The Effect of Chlorogenic Acid on Based on Metabolomics.

Molecules 2020 Sep 4;25(18). Epub 2020 Sep 4.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China.

Chlorogenic acid (CGA), a natural phenolic compound, is an important bioactive compound, and its antibacterial activity has been widely concerned, but its antibacterial mechanism remains largely unknown. Protein leakage and the solution exosmosis conductivity of () reportedly display no noticeable differences before and after CGA treatment. The bacterial cells treated with CGA displayed a consistently smooth surface under the electron microscope, indicating that CGA cannot directly disrupt bacterial membranes. However, CGA induced a significant decrease in the intracellular adenosine triphosphate (ATP) concentration, possibly by affecting the material and energy metabolism or cell-signaling transduction. Furthermore, metabolomic results indicated that CGA stress had a bacteriostatic effect by inducing the intracellular metabolic imbalance of the tricarboxylic acid (TCA) cycle and glycolysis, leading to metabolic disorder and death of . These findings improve the understanding of the complex action mechanisms of CGA antimicrobial activity and provide theoretical support for the application of CGA as a natural antibacterial agent.
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http://dx.doi.org/10.3390/molecules25184038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571229PMC
September 2020

Genetic Dissection of the General Combining Ability of Yield-Related Traits in Maize.

Front Plant Sci 2020 22;11:788. Epub 2020 Jul 22.

Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

Maize yield components including row number, kernel number per row, kernel thickness, kernel width, kernel length, 100-kernel weight, and volume weight affect grain yield directly. Previous studies mainly focused on dissecting the genetic basis of performances for yield-related traits, but the genetic basis of general combining ability (GCA) for these traits is still unclear. In the present study, 328 RILs were crossed as males to two testers according to the NCII mating design, resulting in a hybrid panel composed of 656 hybrids. Both the hybrids and parental lines were evaluated in four environments in 2015 and 2016. Correlation analysis showed the performances of GCA effects were significantly correlated to the performances of RILs for all yield-related traits (0.17 ≤ ≤ 0.64, > 0.01). Only 17 of 95 QTL could be detected for both performances of RILs and GCA effects for eight yield-related traits. The QTL and , which could explain more than 10% of the variation in the GCA effects of KN and HKW, were also detected for performances for the traits. The pleiotropic loci and , which together explained 14.92% of the observed variation in GCA effects for RN, were associated with the GCA effects of KW and HKW, but not with performances for these traits. In contrast, , which was related to seed weight in maize, was mapped to the region surrounding MK2567 at the locus, but no GCA effect was detected. The QTL identified in present study for performances and corresponding GCA effects for yield-related traits might be useful for maize hybrid breeding.
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http://dx.doi.org/10.3389/fpls.2020.00788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387702PMC
July 2020