Publications by authors named "Zhenhua Gao"

59 Publications

F-RGD PET/CT and Systemic Inflammatory Biomarkers Predict Outcomes of Patients With Advanced NSCLC Receiving Combined Antiangiogenic Treatment.

Front Oncol 2021 4;11:671912. Epub 2021 Jun 4.

Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.

Background: The aim of this study was to evaluate F-AlF-NOTA-PRGD2 positron emission tomography/computed tomography (F-RGD PET/CT) and serum inflammation biomarkers for predicting outcomes of patients receiving combined antiangiogenic treatment for advanced non-small cell lung cancer (NSCLC).

Methods: Patients with advanced NSCLC underwent F-RGD PET/CT examination and provided blood samples before treatments commenced. PET/CT parameters included maximum standard uptake value (SUVmax) and mean standard uptake value (SUVmean), peak standard uptake value (SUVpeak) and metabolic tumor volume (MTV) for all contoured lesions. Biomarkers for inflammation included pretreatment neutrophil-to-lymphocyte ratio (PreNLR), pretreatment platelet-to-lymphocyte ratio (PrePLR), and pretreatment lymphocyte-to-monocyte ratio (PreLMR). Receiver operating characteristic (ROC) curve analysis was used to describe response prediction accuracy. Logistic regression and Cox's regression analysis was implemented to identify independent factors for short-term responses and progression-free survival (PFS).

Results: This study included 23 patients. According to ROC curve analysis, there were significant correlations between the SUVmax, SUVmean, and F-RGD PET/CT MTV and short-term responses (<0.05). SUVmax was identified using logistic regression analysis as a significant predictor of treatment sensitivity (=0.008). Cox's multivariate regression analysis suggested that high SUVpeak (=0.021) and high PreLMR (=0.03) were independent PFS predictors. Combining SUVpeak and PreLMR may also increase the prognostic value for PFS, enabling us to identify a subgroup of patients with intermediate PFS.

Conclusion: F-RGD uptake on PET/CT and serum inflammation biomarker pretreatment may predict outcomes for combined antiangiogenic treatments for advanced NSCLC patients. Higher F-RGD uptake and higher PreLMR also appear to predict improved short-term responses and PFS. Combining biomarkers may therefore provide a basis for risk stratification, although further research is required.
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http://dx.doi.org/10.3389/fonc.2021.671912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212050PMC
June 2021

Discovery of eight alkaloids with D1 and D2 antagonist activity in leaves of Nelumbo nucifera Gaertn. Using FLIPR assays.

J Ethnopharmacol 2021 Jun 15;278:114335. Epub 2021 Jun 15.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Ethnopharmacological Relevance: Dopamine receptors are long-standing primary targets in the treatment of mental diseases and there is growing evidence that suggests relationships between obesity and the dopamine system, especially dopamine D1 and D2 receptors. Leaves of Nelumbo nucifera Gaertn. (lotus leaves) have been medically used for helping long-term maintenance of weight loss. Whether and how components of lotus leaves function through the dopamine receptors remains unclear.

Aim Of The Study: This work aimed to discover dopamine receptor-active alkaloids isolated from the lotus leaves, to evaluate their potencies and to analyze their structure activity relationship.

Materials And Methods: Dried lotus leaves were prepared and total extract was divided into alkaloids and flavones. Eight alkaloids were separated and characterized by a combination of high-performance liquid chromatography, quadrupole time-of-flight mass spectrometry and nuclear magnetic resonance, and assayed by a fluorometric imaging plate reader platform. Human embryonic kidney 239 cell lines expressing dopamine D1, D2 and serotonin 2A (5-HT2A) receptors, respectively, were cultured and used in the assay.

Results: Alkaloids in the lotus leaves were the bioactive phytochemicals and inhibited dopamine from accessing the D1 and D2 receptors. All eight compounds functioned as D1-receptor antagonists and except N-nornuciferine, seven alkaloids functioned as D2-receptor antagonists. (S)-coclaurine and (R)-coclaurine are optical isomers and antagonized both D1 and D2 with equivalent potencies, suggesting that the optical rotation of the methylene linker in the monobenzyl isoquinoline backbone did not influence their activity. Among the eight alkaloids, O-nornuciferine was the potent antagonist to both receptors (the lowest IC values, D1: 2.09 ± 0.65 μM and D2: 1.14 ± 0.10 μM) while N-nornuciferine was found to be the least potent as it moderately antagonized D1 and was inactive on D2. O-nornuciferine was also a 5-HT2A antagonist (IC~20 μM) while N-nornuciferine had no activity. These hinted the importance of a methyl group attached to the nitrogen atom in the aporphine backbone. Armepavine showed a nearly 10-fold selectivity to D2.

Conclusions: In this work, eight alkaloids were isolated from the leaves of Nelumbo nucifera Gaertn. and assayed on the D1 and D2 receptors. They were D1/D2 antagonists with IC values in the mid- to low-micromolar range and O-nornuciferine was the most potent alkaloid among the eight. This family of alkaloids was biochemically evaluated on the dopamine receptors by the same platform for the first time.
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http://dx.doi.org/10.1016/j.jep.2021.114335DOI Listing
June 2021

Computed Tomography-Based Delta-Radiomics Analysis for Discriminating Radiation Pneumonitis in Patients With Esophageal Cancer After Radiation Therapy.

Int J Radiat Oncol Biol Phys 2021 May 8. Epub 2021 May 8.

Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China. Electronic address:

Purpose: Our purpose was to construct a computed tomography (CT)-based delta-radiomics nomogram and corresponding risk classification system for individualized and accurate estimation of severe acute radiation pneumonitis (SARP) in patients with esophageal cancer (EC) after radiation therapy.

Methods And Materials: Four hundred patients with EC were enrolled from 2 independent institutions and were divided into the training (n = 200) and validation (n = 200) cohorts. Eight hundred fifty radiomics features of lung were extracted from treatment planning images, including the positioning CT before radiation therapy (CT) and the resetting CT after receiving 40 to 45 Gy (CT). The longitudinal net changes in radiomics features from CT to CT were calculated and defined as delta-radiomics features. Least absolute shrinkage and selection operator algorithm was performed to features selection and delta-radiomics signature building. Integrating the signature with multidimensional clinicopathologic, dosimetric, and hematological predictors of SARP, a novel CT-based delta-radiomics nomogram was established according to multivariate analysis. The clinical application values of nomogram were both evaluated in the training and validation cohorts by concordance index, calibration curves, and decision curve analysis. Recursive partitioning analysis was used to generate a risk classification system.

Results: The delta-radiomics signature consisting of 24 features was significantly associated with SARP status (P < .001). Incorporating it with other high-risk factors, Subjective Global Assessment score, pulmonary fibrosis score, mean lung dose, and systemic immune inflammation index, the developed delta-radiomics nomogram showed increased improvement in SARP discrimination accuracy with concordance index of 0.975 and 0.921 in the training and validation cohorts, respectively. Calibration curves and decision curve analysis confirmed the satisfactory clinical feasibility and utility of nomogram. The risk classification system displayed excellent performance on identifying SARP occurrence (P < .001).

Conclusions: The delta-radiomics nomogram and risk classification system as low-cost and noninvasive means exhibited superior predictive accuracy and provided individualized probability of SARP stratification for patients with EC.
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http://dx.doi.org/10.1016/j.ijrobp.2021.04.047DOI Listing
May 2021

Practical catalytic enantioselective synthesis of 2,3-dihydroquin-azolinones by chiral brønsted acid catalysis.

Org Biomol Chem 2021 May;19(18):4146-4151

State Key Laboratory of NBC Protection for Civilian, Beijing, P. R. China.

Herein, we report on the highly efficient and practical synthesis of 2,3-dihydroquinazolinones directly from diverse aldehydes with excellent yields and enantioselectivity. Particularly, this protocol affords better enantiocontrol for aliphatic aldehydes (up to 99% yield, 97% ee), which always gave unsatisfactory results in the previous studies. Moreover, this catalytic system shows wide tolerance to different functional groups such as alkenyl, nitro and halogens. Most importantly, its practicability is well elucidated via the gram-scale synthesis of different types of products at 0.1 mol% catalyst loading and the simplified work-up procedure. To better understand the reaction pathway and origin of the enantioselectivity, DFT calculations were also performed.
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http://dx.doi.org/10.1039/d1ob00070eDOI Listing
May 2021

A facile and practical preparation of P-chiral phosphine oxides.

Chem Commun (Camb) 2021 Apr 5;57(27):3335-3338. Epub 2021 Mar 5.

School of Physical Science and Technology, ShanghaiTech University, Shanghai 200031, China.

A practical and cost-effective synthetic method of P-chiral diarylalkyl, aryldialkyl, and triaryl phosphine oxides by using readily available chiral diphenyl-2-pyrrolidinemethanol as the auxiliary is developed. The long-standing racemization issue during solvolysis has been addressed and well controlled by employing a suitable solvent, a low reaction temperature, and an appropriate reaction time.
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http://dx.doi.org/10.1039/d1cc00646kDOI Listing
April 2021

The Predictive Value of Tumor Volume and Its Change on Short-Term Outcome for Esophageal Squamous Cell Carcinoma Treated With Radiotherapy or Chemoradiotherapy.

Front Oncol 2020 1;10:586145. Epub 2021 Feb 1.

Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.

Objectives: To investigate the tumor volume and its change on short-term outcome in esophageal squamous cell carcinoma (ESCC) patients who underwent definitive radiotherapy or chemoradiotherapy.

Methods And Materials: All data were retrospectively collected from 418 ESCC patients who received radiotherapy or chemoradiotherapy at our institution between 2015 and 2019. Short-term outcome using the treatment response evaluation was assessed according to the RECIST 1.1. The tumor volume change rate (TVCR) was defined as follows: TVCR {1 [gross tumor volume (GTV) at shrinking irradiation field planning)]/(GTV at the initial treatment planning)} ×100%. Chi square test was used to compare the clinic characteristics in different TVCR groups, and the difference between initial GTV (GTVi) and shrinking GTV (GTVs) was compared using Wilcoxon's sign rank test. Logistic regression analysis and Spearman correlation was performed.

Results: There was a significant decrease in GTVi compared to GTVs ( < 0.001). In univariate analysis, age, cT-stage, TNM stage, treatment modality, GTVi, and TVCR were associated with short-term outcome (all 0.05). In multivariate analysis, gender and TVCR were statistically significant ( = 0.010, <0.001) with short-term outcome, and the combined predictive value of gender and TVCR exceeded that of TVCR (AUC, 0.876 0.855).

Conclusions: TVCR could serve to forecast short-term outcome of radiotherapy or chemoradiotherapy in ESCC. It was of great significance to guide the individualized treatment of ESCC.
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http://dx.doi.org/10.3389/fonc.2020.586145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901880PMC
February 2021

Osseointegration of a novel dental implant in canine.

Sci Rep 2021 Feb 22;11(1):4317. Epub 2021 Feb 22.

Outpatient Department of Oral and Maxillofacial Surgery, School of Stomatology, Capital Medical University, Tian Tan Xi Li No. 4, Beijing, 100050, People's Republic of China.

This study aimed to compare and verify the osseointegration performance of a novel implant (NI) in vivo, which could provide a useful scientific basis for the further development of NIs. Thirty-two NIs treated with hydrofluoric acid and anodization and sixteen control implants (CIs) were placed in the mandibles of 8 beagles. Micro-CT showed that the trabecular number (Tb.N) significantly increased and trabecular separation (Tb.Sp) significantly decreased in the NIs at 2 weeks. Significant differences were found in the trabecular thickness, Tb.N, Tb.Sp, bone surface/bone volume ratio, and bone volume/total volume ratio between the two groups from the 2nd-4th weeks. However, there were no significant differences between the two groups in the bone volume density at 2, 4, 8, or 12 weeks or bone-implant contact at 2 or 4 weeks, but the BIC in the CIs was higher than that in the NIs at the 8th and 12th weeks. Meanwhile, the histological staining showed a similar osseointegration process between the two groups over time. Overall, the NIs could be used as new potential implants after further improvement.
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http://dx.doi.org/10.1038/s41598-021-83700-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900171PMC
February 2021

Topological-Distortion-Driven Amorphous Spherical Metal-Organic Frameworks for High-Quality Single-Mode Microlasers.

Angew Chem Int Ed Engl 2021 Mar 12;60(12):6362-6366. Epub 2021 Feb 12.

Key Laboratory of photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Metal-organic frameworks (MOFs) have recently emerged as appealing platforms to construct microlasers owing to their compelling characters combining the excellent stability of inorganic materials and processable characters of organic materials. However, MOF microstructures developed thus far are generally composed of multiple edge boundaries due to their crystalline nature, which consequently raises significant scattering losses that are detrimental to lasing performance. In this work, we propose a strategy to overcome the above drawback by designing spherically shaped MOFs microcavities. Such spherical MOF microstructures are constructed by amorphizing MOFs with a topological distortion network through introducing flexible building blocks into the growth environment. With an ultra-smooth surface and excellent circular boundaries, the acquired spherical microcavities possess a Q factor as high as ≈10 and can provide sufficient feedback for high-quality single-mode lasing oscillations. We hope that these results will pave an avenue for the construction of new types of flexible MOF-based photonic components.
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http://dx.doi.org/10.1002/anie.202014033DOI Listing
March 2021

A nomogram to predict short-term outcome of radiotherapy or chemoradiotherapy based on pre/post-treatment inflammatory biomarkers and their dynamic changes in esophageal squamous cell carcinoma.

Int Immunopharmacol 2021 Jan 18;90:107178. Epub 2020 Nov 18.

Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, China; Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China. Electronic address:

Objective: We initially aimed to investigate pre/post-treatment inflammatory biomarkers (pre/post-IBs) and their dynamic changes (delta-IBs) on the short-term outcome (STO) of radiotherapy or chemoradiotherapy in esophageal squamous cell carcinoma (ESCC). Furthermore, a nomogram was built to provide an accurate prediction of STO.

Methods: The STO using the treatment response evaluation was assessed according to RECIST 1.1 at 1 month after radiotherapy or chemoradiotherapy. The IBs (absolute lymphocyte counts (ALC), neutrophil/lymphocyte (NLR), platelet/lymphocyte (PLR), and lymphocyte/monocyte (LMR)) and clinical variables were collected and analyzed from 398 ESCC patients at Shandong Cancer Hospital between 2015 and 2019. The nomogram was then established for predicting STO.

Results: Pre-ALC and pre-LMR significantly increased, pre-NLR and pre-PLR significantly decreased during radiotherapy or chemoradiotherapy (all P < 0.001). Meanwhile, there was a positive correlation between delta-NLR as well as delta-PLR (r = 0.621) and delta-LMR (r = 0.613), whereas a negatively correlated between delta-LMR and delta-PLR (r = -0.573). Multivariate analysis indicated that gender [OR, 0.473; 95%CI, 0.274-0.816; P = 0.007], pre-ALC [OR, 0.554; 95%CI, 0.335-0.915; P = 0.021], pre-NLR [OR, 3.176; 95%CI, 1.733-5.823; P < 0.001], post-NLR [OR, 2.418; 95%CI, 1.271-4.600; P = 0.007] and delta-NLR [OR, 1.929; 95%CI, 1.035-3.595; P = 0.039] were statistically significant with STO. And c-index of the nomogram established by combining all independent predictors for STO was 0.770 [95%CI, 0.719-0.820].

Conclusion: Pre-NLR, pre-ALC, post-NLR, and delta-NLR were significant with STO in ESCC patients treated with radiotherapy or chemoradiotherapy. Further, pre-NLR had the best predictive value, and the developed nomogram with superior prediction ability for STO could assist in patients counseling and guide to make individual treatments.
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http://dx.doi.org/10.1016/j.intimp.2020.107178DOI Listing
January 2021

Application and Prospects of Molecular Imaging in Immunotherapy.

Cancer Manag Res 2020 30;12:9389-9403. Epub 2020 Sep 30.

Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan 250117, Shandong, People's Republic of China.

Recently, immunotherapies that target the interactions of programmed cell death 1 (PD-1) with its major ligands, programmed death ligand 1 (PD-L1) and programmed death ligand 2 (PD-L2), have achieved significant success. To date, several immune checkpoint inhibitors targeting the PD-1/PD-L1 pathway have been developed to treat melanoma, non-small cell lung cancer, head and neck cancer, renal cell carcinoma, and urothelial carcinoma. Despite promising outcomes with immunotherapy, there are many limitations to several current immune biomarkers for predicting immune benefits and to traditional imaging for evaluating the efficacy and prognosis of immunotherapy and monitoring adverse reactions. In this review, we recommend a novel imaging method, molecular imaging. This paper reviews the application and prospects of molecular imaging in the context of current immunotherapies in regard to the following aspects: 1) detecting the expression of PD-1/PD-L1; 2) evaluating the efficacy of immunotherapy; 3) assessing patient prognosis with immunotherapy; 4) monitoring the toxicity of immunotherapy; and 5) other targets imaging.
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http://dx.doi.org/10.2147/CMAR.S269773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533904PMC
September 2020

The arch bridge sign: a newly described CT feature of the coronavirus disease-19 (COVID-19) pneumonia.

Quant Imaging Med Surg 2020 Jul;10(7):1551-1558

Department of Radiology, Shenzhen University General Hospital, Shenzhen University Clinical Medical Academy, Shenzhen, China.

Background: To investigate the distribution of CT features and also to introduce a novel described CT feature of coronavirus disease-19 (COVID-19) pneumonia.

Methods: A series of radiologic signs in 11 COVID-19 patients were summarized and made morphometric analysis.

Results: A special sign termed as "the arch bridge sign" owing to its morphological mimicking an arch bridge was firstly introduced. Statistical analyze showed that the subpleural area is the priority distribution location (14/14) and the sign inclined to perform in those patients in a relatively early stage (6/8) and with moderate clinical severity (8/8). Segment VI in lower lobe involved most (6/14). In this retrospective study, other characteristic radiologic signs of COVID-19 pneumonia were analyzed synchronously. A series of radiologic signs were identified in bilateral lungs with a bias towards segment VI, I + II and X. Segment VI had the largest number of each sign. Ground-glass opacities (GGOs), subpleural distribution pattern and vessels dilatation were the top three most common signs among them.

Conclusions: The recognition of the arch bridge sign may benefit patient care by earlier definitive diagnosis of COVID-19 pneumonia. The lesions of COVID-19 pneumonia distributed mainly in the back-lung segments, which characteristic may light new ideas in clinical treatment and nursing strategy.
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http://dx.doi.org/10.21037/qims-20-688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358413PMC
July 2020

Spatially Responsive Multicolor Lanthanide-MOF Heterostructures for Covert Photonic Barcodes.

Angew Chem Int Ed Engl 2020 Oct 26;59(43):19060-19064. Epub 2020 Aug 26.

Key Laboratory of photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Micro/nanoscale photonic barcodes based on multicolor luminescent segmented heterojunctions hold potential for applications in information security. However, such multicolor heterojunctions reported thus far are exclusively based on static luminescent signals, thus restricting their application in advanced confidential information protection. Reported here is a strategy to design responsive photonic barcodes with heterobimetallic (Tb /Eu ) metal-organic framework multicolor heterostructures. The spatial colors could be precisely controlled by thermally manipulating the energy-transfer process between the two lanthanides, thus achieving responsive covert photonic barcodes. Also demonstrated is that spatially resolved responsive barcodes with multi-responsive features could be created in a single heterostructure. These findings offer unique opportunities to purposely design highly integrated responsive microstructures and smart devices toward advanced anti-counterfeiting applications.
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http://dx.doi.org/10.1002/anie.202009295DOI Listing
October 2020

General Synthesis of Chiral α,α-Diaryl Carboxamides by Enantioselective Palladium-Catalyzed Cross-Coupling.

Org Lett 2020 07 17;22(13):4974-4978. Epub 2020 Jun 17.

State Key Laboratory of Bio-Organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Ling Ling Road, Shanghai 200032, People's Republic of China.

A general synthesis of chiral α,α-diaryl carboxamides is developed by enantioselective cross-coupling between 2-bromo-2-aryl carboxamides and arylboronic acids, leading to a series of chiral α,α-diaryl carboxamides with various electronic properties and functionalities in moderate to excellent enantioselectivities and yields. The employment of a sterically bulky chiral P,P═O ligand is critical for the reactivity and selectivity. This protocol is applied to an efficient asymmetric synthesis of a key intermediate of dopamine receptor agonist SKF 38393.
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http://dx.doi.org/10.1021/acs.orglett.0c01489DOI Listing
July 2020

3D-printing of solvent exchange deposition modeling (SEDM) for a bilayered flexible skin substitute of poly (lactide-co-glycolide) with bioorthogonally engineered EGF.

Mater Sci Eng C Mater Biol Appl 2020 Jul 8;112:110942. Epub 2020 Apr 8.

Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

Biodegradable polyesters have been widely used as rigid biomedical apparatus because of high mechanical properties but few flexible implants. Herein, we report a flexible poly(lactide-co-glycolide) (PLGA) scaffold using a rapid in situ formation system based on phase separation by solvent exchange deposition modeling (SEDM), which was different from traditional 3D printing of fused deposition modeling (FDM). The FDM printed product was rigidity, its Young's modulus was approximate 2.6 times higher than that of SEDM printed sample. In addition, the thickness of the solidified ink would not shrink during the SEDM printing process, its surface had nano-/micro pores in favor of protein immobilization and cell adhesion. Then a flexible bilayered scaffold with nano-/microstructure was constructed combing SEDM with electrospinning technology for skin substitute, wherein the SEDM printed sample acted as a sub-layer for cell and tissue ingrowth, the densely packed electrospun nanofibers served as an upper-layer improving the sub-layer's tensile strength by 57.07% and preventing from bacteria as physical barrier. Ultimately, the bilayered scaffold immobilized epidermal growth factor (EGF) by a bioorthogonal approach was successfully applied to facilitate full-thickness wound healing of rats.
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http://dx.doi.org/10.1016/j.msec.2020.110942DOI Listing
July 2020

Feasibility of multi-parametric magnetic resonance imaging combined with machine learning in the assessment of necrosis of osteosarcoma after neoadjuvant chemotherapy: a preliminary study.

BMC Cancer 2020 Apr 15;20(1):322. Epub 2020 Apr 15.

Department of Radiology, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China.

Background: Response evaluation of neoadjuvant chemotherapy (NACT) in patients with osteosarcoma is significant for the termination of ineffective treatment, the development of postoperative chemotherapy regimens, and the prediction of prognosis. However, histological response and tumour necrosis rate can currently be evaluated only in resected specimens after NACT. A preoperatively accurate, noninvasive, and reproducible method of response assessment to NACT is required. In this study, the value of multi-parametric magnetic resonance imaging (MRI) combined with machine learning for assessment of tumour necrosis after NACT for osteosarcoma was investigated.

Methods: Twelve patients with primary osteosarcoma of limbs underwent NACT and received MRI examination before surgery. Postoperative tumour specimens were made corresponding to the transverse image of MRI. One hundred and two tissue samples were obtained and pathologically divided into tumour survival areas (non-cartilaginous and cartilaginous tumour viable areas) and tumour-nonviable areas (non-cartilaginous tumour necrosis areas, post-necrotic tumour collagen areas, and tumour necrotic cystic/haemorrhagic and secondary aneurismal bone cyst areas). The MRI parameters, including standardised apparent diffusion coefficient (ADC) values, signal intensity values of T2-weighted imaging (T2WI) and subtract-enhanced T1-weighted imaging (ST1WI) were used to train machine learning models based on the random forest algorithm. Three classification tasks of distinguishing tumour survival, non-cartilaginous tumour survival, and cartilaginous tumour survival from tumour nonviable were evaluated by five-fold cross-validation.

Results: For distinguishing non-cartilaginous tumour survival from tumour nonviable, the classifier constructed with ADC achieved an AUC of 0.93, while the classifier with multi-parametric MRI improved to 0.97 (P = 0.0933). For distinguishing tumour survival from tumour nonviable, the classifier with ADC achieved an AUC of 0.83, while the classifier with multi-parametric MRI improved to 0.90 (P < 0.05). For distinguishing cartilaginous tumour survival from tumour nonviable, the classifier with ADC achieved an AUC of 0.61, while the classifier with multi-parametric MRI parameters improved to 0.81(P < 0.05).

Conclusions: The combination of multi-parametric MRI and machine learning significantly improved the discriminating ability of viable cartilaginous tumour components. Our study suggests that this method may provide an objective and accurate basis for NACT response evaluation in osteosarcoma.
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http://dx.doi.org/10.1186/s12885-020-06825-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161007PMC
April 2020

Gram-Scale Synthesis of 41% Efficient Single-Component White-Light-Emissive Carbonized Polymer Dots with Hybrid Fluorescence/Phosphorescence for White Light-Emitting Diodes.

Adv Sci (Weinh) 2020 Feb 16;7(4):1902688. Epub 2020 Jan 16.

School of Materials Science & Engineering Qilu University of Technology (Shandong Academy of Sciences) Jinan 250300 China.

Fluorescent carbon dots (CDs) are compelling optical emitters to construct white light-emitting diodes (WLEDs). However, it remains a challenge to achieve large-scale and highly efficient single-component white-light-emissive CDs suitable for WLED applications. Herein, a low cost, fast processable, environmentally friendly, and one-step synthetic approach is developed for the preparation of gram-scale and highly efficient single-component white-light-emissive carbonized polymer dots (SW-CPDs). It is revealed that hybrid fluorescence/phosphorescence components cooperatively contribute to the emergence of white light emission. The SW-CPDs exhibit a record quantum yield (QY) of ≈41% for the white light emission observed in solid-state CD systems, while the QY of the phosphorescence is ≈23% under ambient conditions. Heavy doping of N and P elements as well as presence of covalently cross-linked polymer frameworks is suggested to account for the emergence of hybrid fluorescence/phosphorescence, which is supported by the experimental results and theoretical calculations. A WLED is fabricated by applying the SW-CPDs on an UV-LED chip, showing favorable white-light-emitting characteristics with a high luminous efficacy of 18.7 lm W that is comparable to that of state-of-the-art WLEDs reported before.
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http://dx.doi.org/10.1002/advs.201902688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029630PMC
February 2020

Dynamically wavelength-tunable random lasers based on metal-organic framework particles.

Nanoscale 2020 Feb;12(8):4833-4838

School of Materials Science & Engineering, Qilu University of Technology (Shandong Academy of Sciences), 3501 Daxue Road, Changqing District, Jinan 250353, Shandong Province, China.

We propose a strategy to construct dynamically tunable random lasers by continuously adjusting the excited state of gain molecules spatially confined in the nanoporous channels of metal-organic framework particles. Wavelength-tunable random lasers are achieved by thermally manipulating the intramolecular charge transfer process of gain molecules. The wavelength-tunable response to thermal stimuli exhibits excellent reversible behavior. We envisage that such random lasers based on metal-organic frameworks will raise new fundamental issues regarding light-matter interactions in complex photonic media and open up a new avenue toward highly efficient light-emitting devices.
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http://dx.doi.org/10.1039/c9nr09644bDOI Listing
February 2020

Liquid crystalline networks based on photo-initiated thiol-ene click chemistry.

Soft Matter 2020 Feb 20;16(7):1760-1770. Epub 2019 Dec 20.

Materials Science and Technology Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN 37831, USA.

Photo-initiated thiol-ene click chemistry is used to develop shape memory liquid crystalline networks (LCNs). A biphenyl-based di-vinyl monomer is synthesized and cured with a di-thiol chain extender and a tetra-thiol crosslinker using UV light. The effects of photo-initiator concentration and UV light intensity on the curing behavior and liquid crystalline (LC) properties of the LCNs are investigated. The chemical composition is found to significantly influence the microstructure and the related thermomechanical properties of the LCNs. The structure-property relationship is further explored using molecular dynamics simulations, revealing that the introduction of the chain extender promotes the formation of an ordered smectic LC phase instead of agglomerated structures. The concentration of the chain extender affects the liquid crystallinity of the LCNs, resulting in distinct thermomechanical and shape memory properties. This class of LCNs exhibits fast curing rates, high conversion levels, and tailorable liquid crystallinity, making it a promising material system for advanced manufacturing, where complex and highly ordered structures can be produced with fast reaction kinetics and low energy consumption.
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http://dx.doi.org/10.1039/c9sm01818bDOI Listing
February 2020

Construction of Polymer Electrolyte Based on Soybean Protein Isolate and Hydroxyethyl Cellulose for a Flexible Solid-State Supercapacitor.

Polymers (Basel) 2019 Nov 17;11(11). Epub 2019 Nov 17.

Materials Science and Engineering College, Northeast Forestry University, Harbin 150040, China.

Supercapacitors are a very active research topic. However, liquid electrolytes present several drawbacks on security and packaging. Herein, a gel polymer electrolyte was prepared based on crosslinked renewable and environmentally friendly soybean protein isolate (SPI) and hydroxyethyl cellulose (HEC) with 1.0 mol L LiSO. Highly hydrophilic SPI and HEC guaranteed a high ionic conductivity of 8.40 × 10 S cm. The fabricated solid-state supercapacitor with prepared gel polymer electrolyte exhibited a good electrochemical performance, that is, a high single electrode gravimetric capacitance of 91.79 F g and an energy density of 7.17 W h kg at a current density of 5.0 A g. The fabricated supercapacitor exhibited a flexible performance under bending condition superior to liquid supercapacitor and similar electrochemical performance at various bending angles. In addition, it was proved by an almost 100% cycling retention and a coulombic efficiency over 5000 charge-discharge cycles. For comparison, supercapacitors assembled with commercial aqueous PP/PE separator, pure SPI membrane, and crosslinked SPI membrane were also characterized. The obtained gel polymer electrolyte based on crosslinked SPI and HEC may be useful for the design of advanced polymer electrolytes for energy devices.
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http://dx.doi.org/10.3390/polym11111895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918148PMC
November 2019

Purification of tertiary and quaternary alkaloids from Rhizoma Corydalis using reversed-phase/weak cation-exchange mixed-mode class separation combined with preparative C18 and silica based strong cation-exchange chromatography.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Sep 31;1126-1127:121742. Epub 2019 Jul 31.

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; DICP-CMC Innovation Institute of Medicine, Taizhou 225300, China. Electronic address:

A new optimization strategy for purification of alkaloids from Rhizoma Corydalis using preparative liquid chromatography was developed, featuring a selective separation of different types of alkaloids into different parts by a reversed-phase/weak cation-exchange mixed-mode column (named C18WCX) at first. The total alkaloids of Rhizoma Corydalis were divided into four fractions with fraction III and IV corresponding to the tertiary type medium bases and the quaternary type strong bases, respectively. For fraction III, a conventional C18 column was used to isolate tertiary alkaloids using acetonitrile and 0.1% phosphoric acid (adjusted with triethylamine to pH 6.0) as mobile phases. High selectivity and symmetrical peak shapes of tertiary alkaloids were obtained, resulting in six main tertiary alkaloids isolated in a single run. As strong bases, quaternary alkaloids often suffer from serious peak tailing problem on conventional C18 columns. Therefore, a silica-based strong cation-exchange (SCX) column was used for purification of fraction IV. On the SCX column, good peak shapes in high sample loading were achieved. Five main quaternary alkaloids were isolated and identified from the fraction in one-step. The procedures presented effective for the preparative isolation and purification of alkaloids from Rhizoma Corydalis.
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http://dx.doi.org/10.1016/j.jchromb.2019.121742DOI Listing
September 2019

Heteroepitaxial Growth of Multiblock Ln-MOF Microrods for Photonic Barcodes.

Angew Chem Int Ed Engl 2019 Sep 19;58(39):13803-13807. Epub 2019 Aug 19.

Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Micro/nanoscale multicolor barcodes with unique identifiability and a small footprint play significant roles in applications such as multiplexed labeling and tracking systems. Now, a strategy is reported to design multicolor photonic barcodes based on 1D Ln-MOF multiblock heterostructures, where the domain-controlled emissive colors and different block lengths constitute the fingerprint of a corresponding heterostructure. The excellent heteroepitaxial growth characteristics of MOFs enable the effective modulation of the coding structures, thereby remarkably increasing the encoding capacity. The as-prepared multicolor barcodes enable an efficient authentication and exhibit great potential in fulfilling the functions of anti-counterfeiting, information security, and so on. The results will pave an avenue to novel hybrid MOFs for optical data recording and security labels.
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http://dx.doi.org/10.1002/anie.201907433DOI Listing
September 2019

Exciton funneling in light-harvesting organic semiconductor microcrystals for wavelength-tunable lasers.

Sci Adv 2019 Jun 14;5(6):eaaw2953. Epub 2019 Jun 14.

Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Organic solid-state lasers are essential for various photonic applications, yet current-driven lasing remains a great challenge. Charge transfer (CT) complexes formed with p-/n-type organic semiconductors show great potential in electrically pumped lasers, but it is still difficult to achieve population inversion owing to substantial nonradiative loss from delocalized CT states. Here, we demonstrate the lasing action of CT complexes based on exciton funneling in p-type organic microcrystals with n-type doping. The CT complexes with narrow bandgap were locally formed and surrounded by the hosts with high-lying energy levels, which behave as artificial light-harvesting systems. Excitation light energy captured by the hosts was delivered to the CT complexes, functioning as exciton funnels to benefit lasing actions. The lasing wavelength of such composite microcrystals was further modulated by varying the degree of CT. The results offer a comprehensive understanding of exciton funneling in light-harvesting systems for the development of high-performance organic lasing devices.
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http://dx.doi.org/10.1126/sciadv.aaw2953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6570508PMC
June 2019

Regeneration characteristics of different dental derived stem cell sheets.

J Oral Rehabil 2020 Nov 17;47 Suppl 1:66-72. Epub 2019 Jul 17.

Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.

Background: Although cell sheets have gained much interest as a non-scaffold strategy for tissue regeneration, the regenerative features of different cell sheets remain unclear.

Objective: In this study, we aimed to compare the regeneration characteristics of cell sheets derived from dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs) and stem cells of the apical papilla (SCAPs).

Methods: Dental pulp stem cells, PDLSCs and SCAPs from the same individual were acquired and induced to form sheets using 20 μg/mL vitamin C. Immunofluorescence staining was used to detect the expression of collagen I, fibronectin, integrin β1 and vimentin. Real-time PCR was used to determine NANOG, OCT4, SOX2 and TERT gene expression. The cell sheets with hydroxyapatite/tricalcium phosphate were transplanted into nude mice subcutaneously to evaluate tissue regeneration characteristics.

Results: No obvious differences were found in the histological structure and extracellular matrix protein expression between DPSC, PDLSC and SCAP sheets. Dental pulp stem cell sheet showed higher expression of OCT4 and TERT than PDLSC and SCAP sheets. All three cell sheets displayed the ability of mineral tissue formation and highly expressed periostin. The tissue derived from DPSC sheet showed higher CD31 expression and porous fibres compared with that from the others. The tissue fibres formed from PDLSC sheet were directionally arranged, while the tissue derived from SCAP sheet showed highest mineral tissue formation.

Conclusion: Although in vitro DPSC, PDLSC and SCAP cell sheets have similar characteristics, their regenerative characteristics in vivo are different, with each showing potential application for regeneration of different tissues.
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http://dx.doi.org/10.1111/joor.12839DOI Listing
November 2020

Chitosan oligosaccharide-mediated attenuation of LPS-induced inflammation in IPEC-J2 cells is related to the TLR4/NF-κB signaling pathway.

Carbohydr Polym 2019 Sep 13;219:269-279. Epub 2019 May 13.

Department of Veterinary Medicine, College of Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518018, China. Electronic address:

The protective mechanism of chitosan oligosaccharide (COS) against lipopolysaccharides (LPS) -induced inflammatory responses in IPEC-J2 and in mice with DSS dextran sulfate sodium (DSS) -induced colitis is reported. Upon exposure to LPS, the proliferation rate of IPEC-J2 cells markedly decreased, and epithelial cell integrity was compromised. However, COS pretreatment significantly reduced these changes. Low-concentration (200 μg/mL) COS up-regulated Toll-like receptor 4 (TLR4) and nuclear p65 expression, but inhibited LPS-induced expression of nuclear p65, IL-6, and IL-8. Addition of the TLR4 inhibitor reduced nuclear p65, IL-6, and IL-8 expression in IPEC-J2 cells exposed to COS or LPS alone, and a slight up-regulation in nuclear p65 was observed in COS and LPS co-treated cells. Medium-dose COS (600 mg/kg/d) protected against DSS-induced colitis, in which TLR4 and nuclear p65 expression levels were decreased. We postulate that the prevention of both LPS- and DSS -induced inflammatory responses in IPEC-J2 cells and mice by COS are related to the inhibition of the TLR4/NF-κB signaling pathway.
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http://dx.doi.org/10.1016/j.carbpol.2019.05.036DOI Listing
September 2019

The Effect of Thermo-Chemical Treatment on the Water Resistance of Defatted Soybean Flour-Based Wood Adhesive.

Polymers (Basel) 2018 Aug 28;10(9). Epub 2018 Aug 28.

College of Material Science and Engineering, Northeast Forestry University, Harbin 150000, China.

The aim of this study was to effectively improve the water resistance of a defatted soybean flour (DSF)-based adhesive by subjecting DSF to thermo-chemical treatment in the presence of sodium dodecyl sulfate (SDS), and then the crosslinking with epichlorohydrin-modified polyamide (EMPA). The effect of thermo-chemical treatment on the structures and properties of the DSF and DSF-based adhesive were investigated by plywood evaluation, boiling-water-insoluble content, and acetaldehyde value measurements, as well as FTIR, X-ray photoelectron spectroscopic (XPS), X-ray diffraction spectroscopy (XRD), thermogravimetric analysis (TGA), and rheology analyses. The test results revealed that the water resistance of the DSF-based adhesive was significantly improved, attributed to the formation of a solid three-dimensional crosslinked network structure resulted from the repolymerization of DSF, the Maillard reaction between the protein and carbohydrate, and chemical crosslinking between the crosslinker and DSF. Moreover, SDS destroyed the hydrophobic interactions within protein and inhibited macromolecular aggregations during the thermal treatment. Therefore, more reactive groups buried within the globular structure of the soybean protein component of DSF could be released, which supported the repolymerization, Maillard reaction, and chemical crosslinking of DSF, thereby leading to an improved crosslinking density of the cured DSF-based adhesive. In addition, the adhesive composed of thermo-chemically treated DSF and EMPA exhibited preferable viscosity and viscosity stability suitable for the production of wood composites.
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http://dx.doi.org/10.3390/polym10090955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403534PMC
August 2018

Development of a New Index to Assess the Difficulty Level of Surgical Removal of Impacted Mandibular Third Molars in an Asian Population.

J Oral Maxillofac Surg 2019 Jul 14;77(7):1358.e1-1358.e8. Epub 2019 Mar 14.

Department Head, Outpatient Department of Oral and Maxillofacial Surgery, School of Stomatology, Capital Medical University, Beijing, China. Electronic address:

Purpose: This study aimed to develop a straightforward and accurate index of impacted third molar removal difficulty through analyzing various factors to assess the difficulty level of impacted mandibular third molar (IMTM) extraction.

Materials And Methods: This prospective cohort study included 203 patients who required IMTM extraction. All patients were selected using the preset selection criteria. The present study assessed operation difficulty with operating time. A mathematical model and regression analysis were performed to explore 6 main factors (age, number of roots, degree of bone impaction, shape of roots, and impaction angle and its relation). Appropriate correction coefficients were obtained to formulate a new IMTM removal difficulty predictive index. Consistency of the κ value was checked to evaluate performance.

Results: Degree of bone impaction had the highest correlation coefficient (0.576), followed by shape of roots (0.359), and the lowest correlation coefficient was for number of roots. The Pederson index for these 203 patients showed that 75, 76, and 52 patients had low, moderate, and high difficulty levels, respectively, whereas the new index categorized 78, 85, and 40 patients as having low, moderate, and high difficulty. Comparison of the Pederson index and new index with operating time showed κ agreements of 65.30 and 77.9% (P < .01), suggesting that the prediction results of the new index are more objective and accurate.

Conclusion: The newly proposed index is straightforward and efficient and exhibited promising results in κ agreement. Because of its straightforward nature, it is better suited for Chinese public hospitals with a large volume of patients who require alveolar surgery. The detection of predictor variables could be useful for graduate students, professionals, and general dental practitioners contemplating IMTM removal to assess the difficulty level of IMTM extraction.
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http://dx.doi.org/10.1016/j.joms.2019.03.005DOI Listing
July 2019

Full-color laser displays based on organic printed microlaser arrays.

Nat Commun 2019 02 20;10(1):870. Epub 2019 Feb 20.

Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, 100190, Beijing, China.

Laser displays, which exploit characteristic advantages of lasers, represent a promising next-generation display technology based on the ultimate visual experience they provide. However, the inability to obtain pixelated laser arrays as self-emissive full-color panels hinders the application of laser displays in the flat-panel sector. Due to their excellent optoelectronic properties and processability, organic materials have great potential for the production of periodically patterned multi-color microlaser arrays. Here, we demonstrate for the first time full-color laser displays on precisely patterned organic red-green-blue (RGB) microlaser matrices through inkjet printing. Individual RGB laser pixels are realized by doping respective luminescent dyes into the ink materials, resulting in a wide achievable color gamut 45% larger than the standard RGB space. Using as-prepared microlaser arrays as full-color panels, we achieve dynamic laser displays for video playing through consecutive beam scanning. These results represent a major step towards full-color laser displays with outstanding color expression.
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http://dx.doi.org/10.1038/s41467-019-08834-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382787PMC
February 2019

Stimulated Emission-Controlled Photonic Transistor on a Single Organic Triblock Nanowire.

J Am Chem Soc 2018 Oct 2;140(41):13147-13150. Epub 2018 Oct 2.

Key Laboratory of Photochemistry , Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190 , China.

In this work, we demonstrate a stimulated emission-controlled photonic transistor on a single organic triblock nanowire composed of alternate energy donor and acceptor. The population of acceptor excitons was engineered by energy transfer to achieve enhanced fluorescence, which was further amplified by the stimulated emission of the donor and the optical feedback in the nanowire microcavities, yielding a remarkable nonlinear amplification of the acceptor emission. On this basis, a prototype of photonic transistor with high nonlinear gain at very low pump energy was achieved. The results will provide a useful enlightenment for the rational design of novel all-optical switches with desired performances.
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http://dx.doi.org/10.1021/jacs.8b04699DOI Listing
October 2018

Diets, obesity and inflammation for cognitive disorder: which is a true risk? Which is a confounding risk?

Authors:
Zhenhua Gao

J Public Health (Oxf) 2019 09;41(3):646-647

Department of Cardiology, Han Dan Central Hospital, No. 15 South of Zhong Hua Street, Han Shan District, Han Dan, People's Republic of China.

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http://dx.doi.org/10.1093/pubmed/fdy162DOI Listing
September 2019

Suppressing Nonradiative Processes of Organic Dye with Metal-Organic Framework Encapsulation toward Near-Infrared Solid-State Microlasers.

ACS Appl Mater Interfaces 2018 Oct 3;10(41):35455-35461. Epub 2018 Oct 3.

College of Chemistry , Beijing Normal University , Beijing 100875 , China.

Organic materials are an important class of gain media for fabricating miniaturized lasers because they combine fabrication simplicity with wide spectral coverage and tunability. However, progress toward near-infrared (NIR) organic solid-state lasers has been limited because of serious nonradiative processes originating from the severe intermolecular interaction in the condensed state. Here, we develop a strategy to realize room-temperature NIR microscale lasers through encapsulating organic dyes into the cavities of metal-organic frameworks (MOFs). The spatial confinement of the dye molecules within the MOF pores contributes to suppressing the multiple nonradiative processes (i.e., aggregation-caused quenching and exciton-exciton annihilation). This results in a much higher radiative efficiency and thus much easier population inversion and low-threshold NIR lasing. Furthermore, the lasing wavelength can be further expanded based on the tailorable energy levels of the dye molecules. The results will provide useful enlightenment for the development of miniaturized NIR laser sources for new photonic applications.
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http://dx.doi.org/10.1021/acsami.8b13566DOI Listing
October 2018