Publications by authors named "Zhengzong Wu"

37 Publications

Effect of chain length on the structure and physicochemical properties of active compound/linear dextrin composites.

Carbohydr Polym 2021 Oct 6;269:118304. Epub 2021 Jun 6.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China; School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.

Three linear dextrins (LDs) with different chain length were obtained through fractionating short-chain LD by gradient precipitation with 65%, 70% and 75% alcohol, signed as 65LD, 70LD, and 75LD, respectively. The LDs were employed to encapsulate arachidonic acid (ARA) and geranic acid (GA). Fourier infrared spectrometry, differential scanning calorimetry and thermogravimetric analysis confirmed the formation of ARA/LD and GA/LD composites. The ARA/65LD and GA/75LD composites showed higher crystallinity, indicating the formation of more ordered and compact structures in ARA/65LD and GA/75LD composites. The micromorphology of ARA/65LD composite was a clear ellipsoidal like structure, whereas GA/75LD composite presented a spherical shape. The release behavior, thermal, photochemical and antioxidant abilities of ARA and GA were improved after forming composites. Overall, the longer chain LD displayed better encapsulation efficiency with the longer chain ARA, while the shorter chain LD had higher encapsulation capability with GA.
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http://dx.doi.org/10.1016/j.carbpol.2021.118304DOI Listing
October 2021

High-Amylose Corn Starch/Konjac Glucomannan Composite Film: Reinforced by Incorporating β-Cyclodextrin.

J Agric Food Chem 2021 Mar 17;69(8):2493-2500. Epub 2021 Feb 17.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.

Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties. The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly. The thickness and transparency of the films increased after β-CD was incorporated. More associations of HCS/KGM enhanced the mechanical properties and reduced the moisture content of the films. The water vapor permeability of the HCS/KGM composite film was also improved significantly with the incorporation of β-CD. The enhanced association between biopolymers in the presence of β-CD will advance the development of a degradable active composite packaging film.
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http://dx.doi.org/10.1021/acs.jafc.0c06648DOI Listing
March 2021

Corrigendum to "Physicochemical properties of pea starch-lauric acid complex modified by maltogenic amylase and pullulanase" [Carbohydr. Polym. 242 (2020) 116332].

Carbohydr Polym 2021 Apr 12;257:117629. Epub 2021 Jan 12.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong 250353, China; School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong 250353, China. Electronic address:

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http://dx.doi.org/10.1016/j.carbpol.2021.117629DOI Listing
April 2021

Effects of ultrasonication on the properties of maize starch/stearic acid/ sodium carboxymethyl cellulose composite film.

Ultrason Sonochem 2021 Apr 28;72:105447. Epub 2020 Dec 28.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong 250353, China; School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong 250353, China. Electronic address:

Ultrasonic treatment can improve the compatibility between a hydrophobic material and a hydrophilic polymer. The light transmittance, crystalline structure, microstructure, surface morphology, moisture barrier, and mechanical properties of a composite film with or without ultrasonication were investigated. Ultrasound increases the film's light transmittance, resulting in a film that has good transparency. Ultrasonication did not change the crystalline structure of the polymer film, but promoted V-type complex formation. The surface of the film became smooth and homogeneous after the film-form suspension underwent ultrasonic treatment. Compared to the control film, after ultrasonication at 70% amplitude with a duration of 30 min, the average roughness and maximum roughness declined from 212 nm to 17.6 nm and from 768.7 nm to 86.5 nm, respectively. The composite film with ultrasonication exhibited better tensile and moisture barrier properties than the nonsonicated film. However, long-term and strong ultrasonication will destroy the polymer structure to some extent.
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http://dx.doi.org/10.1016/j.ultsonch.2020.105447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803932PMC
April 2021

Influence of two functional dextrins on the gel properties of kappa-carrageenan.

Food Res Int 2020 12 18;138(Pt A):109666. Epub 2020 Sep 18.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China; School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.

The physicochemical properties of κ-carrageenan (KC) can be improved by incorporation with small-molecule cosolvents. The texture and rheological properties, micromorphology, and crystallinity of KC incorporating indigestible dextrin (IDD) and beta-limit dextrin (BLD) were investigated. The rheological properties and sol-gel transition temperatures of the gels were slightly improved and the hardness of KC gels was significantly increased after the two dextrins were mixed in. Fourier transform infrared spectroscopy results indicated hydrogen-bonding interactions were strengthened in the presence of the dextrins. Confocal laser scanning microscope images revealed that a more homogenous structure was formed of the KC gel after the addition of dextrins. Moreover, X-ray diffraction patterns indicated the crystallinity of KC gel decreased upon dextrin addition. At the same dextrin content, IDD exerted a greater influence than BLD. IDD contents exceeding 3% (w/w) led to undesirable effects, whereas up to 5% (w/w) of BLD could be added. The two dextrins affected the rearrangement of the KC random coils in the sol state, and facilitated aggregation of the KC chains during cooling to form gel network structures after gelation. Therefore, the appropriate addition of these two dextrins can improve the texture and stability of KC gels and expand their application in functional foods.
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http://dx.doi.org/10.1016/j.foodres.2020.109666DOI Listing
December 2020

Lycium barbarum polysaccharide attenuates myocardial injury in high-fat diet-fed mice through manipulating the gut microbiome and fecal metabolome.

Food Res Int 2020 12 15;138(Pt B):109778. Epub 2020 Oct 15.

State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China. Electronic address:

High-fat diets (HFDs) can induce health problems including gut microbiota dysbiosis and cardiac dysfunction. In this study, we modulated the gut microbiota in mice to investigate whether Lycium barbarum polysaccharide (LBP), a potential prebiotic fiber, could alleviate HFD-induced myocardial injury. Mice fed a HFD were given LBP (HFPD group) by gavage once/day for 2 months. Left ventricular function and serum trimethylamine N-oxide were significantly improved in HFPD mice compared with HFD mice. HFD increased the abundances of Bifidobacterium, Lactobacillus, and Romboutsia, while LBP increased the abundances of Gordonibacter, Parabacteroides, and Anaerostipes. Fecal metabolic profiling revealed significant increases in metabolites involved in nicotinate, nicotinamide and purine metabolism pathways, as well as indole derivatives of tryptophan metabolites in the HFPD group. LBP reduced intestinal permeability and inflammatory cytokine levels, maintained a healthy intestinal microenvironment, and alleviated myocardial injury. Modulating the gut microbiota is a potential treatment for cardiovascular diseases.
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http://dx.doi.org/10.1016/j.foodres.2020.109778DOI Listing
December 2020

Formation of high amylose corn starch/konjac glucomannan composite film with improved mechanical and barrier properties.

Carbohydr Polym 2021 Jan 6;251:117039. Epub 2020 Sep 6.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China; School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China.

Recently, starch-based packaging materials have become one of research hot points. In the present study, glycerol-plasticized composite films based on high amylose corn starch (HCS) and konjac glucomannan (KGM) were developed. The influence of KGM on the film-forming properties of HCS and the physicochemical properties of the resulting films were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TG) analysis and water vapor permeability (WVP). The crystallinity and the proportion of short-range order structure of the films increased first and then declined with the addition of KGM. The micromorphology of the films exhibited the more even texture after KGM was incorporated in. The tensile strength, elongation at break and water resistance of HCS film were also improved significantly. The synergistic effect between HCS and KGM improved the film-forming ability of HCS. The optimal addition amount of KGM was 0.3 %.
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http://dx.doi.org/10.1016/j.carbpol.2020.117039DOI Listing
January 2021

Preparation and characterization of zein/carboxymethyl dextrin nanoparticles to encapsulate curcumin: Physicochemical stability, antioxidant activity and controlled release properties.

Food Chem 2021 Mar 26;340:127893. Epub 2020 Aug 26.

Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China; School of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China. Electronic address:

In this work, zein/carboxymethyl dextrin nanoparticles were successfully fabricated at different zein to carboxymethyl dextrin (CMD) mass ratios. Zein/CMD nanoparticles with the negative charge and the smallest size (212 nm) were formed when the mass ratio of zein to CMD was 2:1, exhibiting improved encapsulation efficiency of curcumin (85.5%). Electrostatic interactions, hydrogen bonding and hydrophobic interactions were main driven forces for nanoparticles formulation and curcumin encapsulation. Fourier transform infrared spectroscopy determined curcumin might be partially embedded in CMD during encapsulation. The spherical structures of zein/CMD nanoparticles and curcumin-loaded zein/CMD nanoparticles were observed by transmission electron microscopy. The photothermal stability and antioxidant activity of curcumin were significantly enhanced after be loaded in zein/CMD nanoparticles. Furthermore, encapsulation of curcumin in zein/CMD nanoparticles significantly delayed the release of curcumin in simulated gastrointestinal fluids. These results indicated that zein/CMD nanoparticles could be effective encapsulating materials for bioactive compounds in food industry.
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http://dx.doi.org/10.1016/j.foodchem.2020.127893DOI Listing
March 2021

Trimer-based aptasensor for simultaneous determination of multiple mycotoxins using SERS and fluorimetry.

Mikrochim Acta 2020 08 14;187(9):495. Epub 2020 Aug 14.

State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China.

An aptasensor is reported for the detection of three different kinds of mycotoxins, i.e., zearalenone (ZEN), ochratoxin A (OTA), and fumonisin B1 (FB1). Based on fluorescence resonance energy transfer effect (FRET) and surface-enhanced Raman scattering (SERS), the levels of ZEN, FB1, and OTA can be simultaneously determined. Under 980-nm and 650-nm laser excitation, the logarithmic values of fluorescence signal intensities at 543 nm and 670 nm are slowly increased as the concentrations of ZEN and OTA vary from 0.1 ng mL and 0.05 ng mL to 100 ng mL and 25 ng mL, respectively. For FB1, under 980-nm laser excitation, the logarithmic value of SERS signal intensity at 1567 cm gradually increases with the concentration of FB1 in the range 0.05-200 pg mL (R = 0.996). The detection limits of the proposed assay for ZEN, OTA, and FB1 are 0.03 ng mL, 0.01 ng mL, and 0.02 pg mL, respectively. The selectivity experiment results indicate this assay possesses a high selectivity over other commonly encountered mycotoxins. The average recoveries range from 90 to 107%, revealing satisfactory application potential of the proposed assay. The developed aptasensor will bring bright prospects for research in the field of multiplexed mycotoxine detection. Graphical Abstract Schematic representation of an aptamer-based assay for multiple mycotoxins determination.
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http://dx.doi.org/10.1007/s00604-020-04487-1DOI Listing
August 2020

Physicochemical properties of pea starch-lauric acid complex modified by maltogenic amylase and pullulanase.

Carbohydr Polym 2020 Aug 21;242:116332. Epub 2020 Apr 21.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong, 250353, China; School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong, 250353, China. Electronic address:

An enzymatic method was investigated to initiate a strategy of increasing the branch density of pea starch, thus facilitating the formation of a starch-lipid complex after debranching. When the starch was modified by exposure to maltogenic amylase (MAL) and pullulanase (PUL), lower molecular weight values and higher amylose content resulted compared to the untreated sample. High-performance anion-exchange chromatography results suggested that the average chain length decreased and the branch density increased after the starch received MAL treatment. The diffraction intensities and the total melting enthalpies of the dual-enzyme treated complex were greater than those for other samples when the level of MAL between 4-12 U/g. Fourier transform infrared spectroscopy revealed that more ordered structure was formed in MAL (4/8/12)-PUL-starch-lauric acid (Lau) complexes. Digestive performance analysis indicated that the enzyme resistance of the starch-Lau complex was reinforced by applying the MAL-PUL modification to the starch.
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http://dx.doi.org/10.1016/j.carbpol.2020.116332DOI Listing
August 2020

Effect of anion type on enzymatic hydrolysis of starch-(thermostable α-amylase)-calcium system in a low-moisture solid microenvironment of bioextrusion.

Carbohydr Polym 2020 Jul 22;240:116331. Epub 2020 Apr 22.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China; National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Integrated Research Base of Southern Fruit and Vegetable Preservation Technology, Zhejiang International Scientific and Technological Cooperation Base of Health Food Manufacturing and Quality Control, Zhejiang University, Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China. Electronic address:

The effect of six anions (Cl-, OH-, NO-, SO-, CHO- and PO-) on a starch (St)-enzyme (thermostable α-amylase, TαA)-calcium (Ca) system was investigated in a low-moisture solid state. Two levels of Ca salts (1 and 10 mmol/100 g St) added to potato starch with and without TαA were analyzed by FT-IR, DSC and SEM. The surface morphologies of the St-Ca complexes were different in the presence of various anions, and the residual Ca salts around the St granules might decrease the enzymatic action. For bioextrusion, TαA (0.5‰ and 1.5‰) were introduced for a relatively low Ca content (1 mmol/100 g). Significant differences in enzyme activity were observed, increasing the activity of TαA by SO- (146.54 %) > CHO- > Cl- > control > NO- > OH- ≈ PO- and CHO- (123.20 %) ≈ Cl- ≈ SO- > control > PO > OH- > NO- for the low and high enzyme levels, respectively.
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http://dx.doi.org/10.1016/j.carbpol.2020.116331DOI Listing
July 2020

A fluorometric method for aptamer-based simultaneous determination of two kinds of the fusarium mycotoxins zearalenone and fumonisin B making use of gold nanorods and upconversion nanoparticles.

Mikrochim Acta 2020 04 1;187(4):254. Epub 2020 Apr 1.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

An aptamer-based assay for the determination of two different kinds of fusarium mycotoxins, i.e., zearalenone (ZEN) and fumonisin B (FB), is presented. Based on the inner filter effect (IFE) strategy, the contents of ZEN and FB can be simultaneously quantified. It is making use of 65-nm gold nanorods (AuNRs), 20-nm upconversion nanoparticles (UCNPs), fluorescence dyes, and DNA sequences. In the absence of ZEN and FB, the UCNPs and AuNRs associate through DNA sequences. Due to IFE effect, weak fluorescence signals are collected. In the presence of ZEN or FB, UCNPs and AuNRs become unstable and partially separate from each other. This results in the recovery of fluorescence signals. Under 980-nm laser excitation, the logarithmic values of fluorescence signal intensities at 606 nm and 753 nm gradually increase with the concentration of ZEN and FB in the ranges 0.05-100 μg L (the coefficient of determination is 0.997) and 0.01-100 ng L (the coefficient of determination is 0.986), respectively. The limits of detection (LOD) of the fabricated assay for ZEN and FB1 are 0.01 μg L and 0.003 ng L, respectively. The proposed method has a high selectivity over other competitive mycotoxins, including aflatoxin B1, ochratoxin A, patulin and ochratoxin B. The applicability of the assay was evaluated in the determination of ZEN and FB1 contents in spiked corn samples. The average recoveries ranged from 89.9 to 106.6%. This result confirms the practicality of this method. Graphical abstract Schematic representation of an aptamer-based fluorometric method for simultaneous determination of two kinds of the fusarium mycotoxins zearalenone and fumonisin B.
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http://dx.doi.org/10.1007/s00604-020-04236-4DOI Listing
April 2020

Preparation, characterization, and encapsulation capability of the hydrogel cross-linked by esterified tapioca starch.

Int J Biol Macromol 2020 Jul 16;155:1-5. Epub 2020 Mar 16.

Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China; School of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China. Electronic address:

The modified starch-based hydrogels were prepared by crosslinking modified starch with sodium trimetaphosphate. Modified starch was obtained by esterification of tapioca starch with maleic anhydride. The degree of substitution (DS) increased significantly from 0.078 to 0.258 as the content of maleic anhydride increased from 6.67% to 33.33%. Fourier transform infrared spectroscopy demonstrated that starch was successfully esterified. In addition, the thermal properties of modified starch-based hydrogels were investigated by differential scanning calorimeter and thermogravimetry analysis, which proved that hydrogels had better thermal stability. Esterified starch-based hydrogels showed excellent pH sensitivity by measuring of swelling degree. When DS was 0.250, the adsorption capacity and encapsulation efficiency of starch-based hydrogels were 399.23 μg/g and 80%, respectively, which exhibited satisfactory embedding properties for curcumin. Therefore, esterified tapioca starch-based hydrogels could be as the encapsulating materials to protect bioactive substances, which provided a theoretical basis for their application in food field and pharmaceuticals industry.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.03.141DOI Listing
July 2020

Aptamer and gold nanorod-based fumonisin B1 assay using both fluorometry and SERS.

Mikrochim Acta 2020 03 11;187(4):215. Epub 2020 Mar 11.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

An aptamer-based assay is presented for the determination of fumonisin B1 (FB1). It is bimodal in that both surface-enhanced Raman spectroscopy (SERS) and fluorometry are applied for quantitation. It makes use of platinum-coated gold nanorod (AuNR) and DNA sequences. The complementary DNA of aptamer (cDNA) against FB1 is immobilized on the surface of AuNR. The aptamer of FB1 modified with Cy5.5 are complementarily hybridized with cDNA. In the absence of FB1, the aptamer and its cDNA associate. In this situation, strong SERS and weak fluorescence signals are obtained. In the presence of FB1, the aptamer disassociates with its cDNA and binds the target. As the concentration of FB1 increases, the SERS and fluorescence signal intensities of the mixture are gradually decreased and increased, respectively. Under optimized conditions, the SERS signal at 1366 cm decreases linearly in the 10-500 pg mL concentration range with the calibration equation of y = 1997lgx-594 (the coefficient of determination is 0.998). The fluorescence signal at 670 nm increases linearly in the 10-250 pg mL concentration range with the calibration equation of y = 500lgx-383 (the coefficient of determination is 0.991). The assay was applied to the determination of FB1 contents in spiked corn samples. The average recoveries ranged from 92 to 107%, confirming the practicality of this method. The results obtained by this assay are in good agreement with that of LC-MS/MS method. Graphical abstractSchematic illustration of a bimodal aptasensor based on surface enhanced Raman scattering (SERS) and fluorescence change for the detection of fumonisin B1 (FB1).
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http://dx.doi.org/10.1007/s00604-020-4192-0DOI Listing
March 2020

Chitosan hydrochloride/carboxymethyl starch complex nanogels stabilized Pickering emulsions for oral delivery of β-carotene: Protection effect and in vitro digestion study.

Food Chem 2020 Jun 25;315:126288. Epub 2020 Jan 25.

Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China; School of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China; State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, PR China. Electronic address:

β-Carotene was encapsulated in the Pickering emulsions stabilized by chitosan hydrochloride - carboxymethyl starch (CHC-CMS) nanogels. During ultraviolet radiation and storage, the retention of β-carotene in Pickering emulsions was higher than that of other formulations, such as Tween 80 stabilized emulsions (TEs) and bulk oil. The Pickering emulsions were found to be stable during thermal treatment. Meanwhile, lipid oxidation was delayed in Pickering emulsions compared to TEs and bulk oil. The vitro digestion results suggested that the free fatty acids (FFA) released were below 30% for all Pickering emulsions, which indicated that a physical barrier was formed by CHC-CMS nanogels to restrain the lipid hydrolysis. The bioaccessibility of β-carotene in Pickering emulsions was higher than that in bulk oil. This research helped establish a connection between the physicochemical properties of CHC-CMS stabilized Pickering emulsions with their applications in the protection effect and oral delivery of bioactive compounds.
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http://dx.doi.org/10.1016/j.foodchem.2020.126288DOI Listing
June 2020

Magnetic (Zn-St)Fe ( = 1, 2, 3, 4) Framework of Macro-Mesoporous Biomaterial Prepared via Green Enzymatic Reactive Extrusion for Dye Pollutants Removal.

ACS Appl Mater Interfaces 2019 Nov 31;11(46):43553-43562. Epub 2019 Oct 31.

College of Biosystems Engineering and Food Science , Zhejiang University , Hangzhou 310058 , China.

Biobased materials have the potential to be developed into green multifunctional products to replace their chemosynthetic counterparts, which have environmental and economic concerns. However, designing magnetic and porous biomaterials without pore spaces being occupied by exogenous magnets via traditional encapsulation, load, and/or deposition methods remains challenging. This paper describes a novel, facile, top-down strategy of fabricating zerovalent iron particles (Fe Ps) embedded into a three-dimensional (3D) zinc-modified starch (Zn-St) framework using the enzymatic reactive extrusion (eREX) method. Raw St underwent Zn-atom fortification, Fe-atom deposition, and micromixing extrusion to produce (Zn-St)Fe ( = 1, 2, 3, 4) extrudates (Es) in a continuous and large-scale mode. A hierarchical porous structure was formed during eREX processing, with mesopores (∼2-4 nm) and macropores (∼50-300 nm and ∼5-100 μm) generated regularly. The (Zn-St)Fe Es were excellent at dye adsorption and magnetic separation, with high levels of St (>70%) as a biodegradable resource. For instance, (Zn-St)Fe Es (St > 83%) removed 61.03 mg/g of methylene blue (∼19 times higher than that of raw St) at 298 K and pH 4.0 via simultaneous physisorption and degradation and were successfully separated due to their saturation magnetization () value of 25.41 emu/g. The dye adsorption rate and of the (Zn-St)Fe Es can be increased by manipulating the amount of Fe Ps. Thus, the novel 3D (Zn-St)Fe Es are promising biomaterials for water purification applications, as well as other food, biological, and environmental fields.
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http://dx.doi.org/10.1021/acsami.9b14750DOI Listing
November 2019

Simultaneous fluorometric and chirality based aptasensing of sulfamethazine by using upconversion nanoparticles and [email protected]@Au core-shell nanoparticles.

Authors:
Zhengzong Wu Bo Cui

Mikrochim Acta 2019 07 20;186(8):555. Epub 2019 Jul 20.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China.

A dual-mode aptasensor was employed for ultrasensitive determination of sulfamethazine (SMZ). The assay is based on simultaneous quantification by using fluorometry and chirality. The aptamer against SMZ was immobilized on upconversion nanoparticles (UCNPs) while the complementary DNA of SMZ aptamer was immobilized on [email protected]@Au core-shell nanoparticles ([email protected]@AuNPs). Based on complementary base-pairing reactions, the aptamer of SMZ and its complementary DNA sequence (cDNA) were hybridized to form duplex structure. Thus, [email protected]@AuNPs and UCNPs were in close proximity. Efficient inner filter effect (IFE) from UCNPs (energy donor) to [email protected]@AuNPs (energy acceptor) occurred under the excitation of 980 nm laser. In the presence of targets (SMZ), as the aptamer of SMZ coupled with SMZ to form stable complex structure. As a result, the hybridization of aptamer and its cDNA deceased and the fluorescence signal recovered. Furthermore, as the degree of the assembly decreased, the circular dichroism (CD) signal also decreased. Fluorescence was measured at excitation/emission wavelengths of 980/655 nm. The linear ranges for detection of SMZ are between 0.10-100 ng·mL and 1.00-100 ng·mL for the fluorescence and circular dichroism modes, respectively. The method was applied to the determination of SMZ in spiked milk with high recoveries. Conceivably, it can be extended to the analysis of numerous other targets for which adequate antibodies or aptamers are available. Graphical abstract Schematic representation of a chirality assay and a fluorometric assay based on inner filter effect (IFE) between upconversion nanoparticles (UCNPs) and [email protected]@Au core-shell nanoparticles ([email protected]@AuNPs) for the determination of sulfamethazine (SMZ).
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http://dx.doi.org/10.1007/s00604-019-3643-yDOI Listing
July 2019

Effects of amylose content and enzymatic debranching on the properties of maize starch-glycerol monolaurate complexes.

Carbohydr Polym 2019 Oct 17;222:115000. Epub 2019 Jun 17.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong 250353, China; School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, Shandong 250353, China.

The effects of pullulanase debranching on the properties of maize starch (waxy, normal and high amylose)-glycerol monolaurate (GML) complexes were studied. Pullulanase pretreatment produced more starch chains with favourable lengths (DP ≥ 25) to encourage inclusion complex formation. The debranched starch showed higher amylose content (from 11.2 to 58.6%) than the native starch (from 0.68 to 53.4%). The V-type characteristic diffraction peaks (7.5°, 13.1° and 20.2° 2θ) became more obvious after the starch received the debranching treatment. Thermal properties indicated that the melting enthalpy of the debranched starch-GML complexes (from 6.45 to 10.13 J/g) was higher than those of the untreated complexes (from 0 to 8.91 J/g). The swelling power values and degree of hydrolysis of the starches decreased with the increase in amylose content. In vitro digestibility analysis suggested that the hydrolysis of the starch-GML samples was further restricted by applying the debranching treatment to the starch.
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http://dx.doi.org/10.1016/j.carbpol.2019.115000DOI Listing
October 2019

Preparation and characterization of emulsion stabilized by octenyl succinic anhydride-modified dextrin for improving storage stability and curcumin encapsulation.

Food Chem 2019 Oct 8;294:326-332. Epub 2019 May 8.

The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

In our study, octenyl succinic anhydride (OSA)-modified dextrin was prepared and characterized as a novel emulsifier to improve the stability of emulsion and curcumin encapsulation. Fourier-transform infrared spectroscopy demonstrated the occurrence of esterification between OSA and dextrin (M = 1.041 × 10 g/mol). The absolute value of ζ-potential of OSA-dextrin increased (from 25.37 mV to 34.57 mV) with increasing OSA addition (from 0% to 8%), and then kept constant. Confocal laser scanning microscope results showed that the debranching and esterification of starch improved the oil droplets distribution and reduced the droplet size of emulsions. The emulsifying stability of emulsions coated by dextrin was greatly improved with OSA modification. The particle size of emulsion decreased significantly when the addition of OSA increased during storage. OSA-modified dextrin was in a position to increase encapsulation efficiency of curcumin. This research may increase the utilization of emulsions stabilized by OSA dextrin in food industry.
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http://dx.doi.org/10.1016/j.foodchem.2019.05.053DOI Listing
October 2019

Establishment of a dual mode immunochromatographic assay for Campylobacter jejuni detection.

Food Chem 2019 Aug 21;289:708-713. Epub 2019 Mar 21.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Campylobacter jejuni (C. jejuni) is considered one of the most common cause of human gastroenteritis. Aiming to detect C. jejuni in food products rapidly and sensitively, a dual mode lateral flow assay, based on the peroxidase mimicking and surface enhanced Raman scattering (SERS) enhancement properties of platinum coated gold nanorods ([email protected]), was developed in this study. Under color mode and SERS mode, the proposed assay showed good linear response in the range of 10-10 cfu/mL and 10-5 × 10 cfu/mL with limits of detection of 75 cfu/mL and 50 cfu/mL, respectively (S/N = 3). Furthermore, the reliability of the dual-readout lateral flow assay (LFA) was successfully demonstrated by the application on milk samples, in which the recoveries ranged from 89.33% to 107.62%. Overall, the immunochromatographic assay developed in this work is promising and has good chance to be employed for sensitive detection of C. jejuni in food products.
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http://dx.doi.org/10.1016/j.foodchem.2019.03.106DOI Listing
August 2019

Ultrasensitive detection of microcystin-LR with gold immunochromatographic assay assisted by a molecular imprinting technique.

Food Chem 2019 Jun 18;283:517-521. Epub 2019 Jan 18.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

A simple and highly sensitive lateral flow immunochromatographic assay (LFICA) towards microcystin-LR (MC-LR) was proposed in this study. Molecular imprinting technique was combined with enzyme assisted colorimetric method to substantially enhance the sensitivity of traditional LFICA. The target, i.e., MC-LR molecular, was first separated from the complex matrix by molecularly-imprinted polymers (MIPs). Then, the obtained MC-LR was detected by LFICA based on the blue color generated by an enzyme-substrate reaction. Using the proposed assay, under optimal conditions, the determination of MC-LR was doable in a wide linear range from 0.1 ng/mL to 100 ng/mL with a detection limit of 0.04 ng/mL. In addition, the reliability of the developed method was validated by determining MC-LR contents in real samples. The overall results suggested that the neoteric LFICA method possess great potential in sensitive detection of MC-LR.
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http://dx.doi.org/10.1016/j.foodchem.2019.01.064DOI Listing
June 2019

A novel SERS-based aptasensor for ultrasensitive sensing of microcystin-LR.

Food Chem 2019 Apr 13;278:197-202. Epub 2018 Nov 13.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

We developed a novel aptasensor based on surface enhanced Raman spectroscopy (SERS) and applied it for highly sensitive detection of microcystin-LR (MC-LR). In this work, MC-LR aptamer and its corresponding complementary DNA fragments (cDNA) were conjugated to gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs), respectively. Then, MC-LR aptamer-AuNPs and cDNA-MNPs conjugates were used as signal probes and capture probes, respectively. The proposed assay exhibited a linearity range from 0.01 to 200 ng/mL with the limit of detection (LOD) of 0.002 ng/mL. In addition, the reliability of the novel approach was validated by detecting different levels of MC-LR spiked in tap water samples. Overall, the novel aptasensor paves a new way for rapid and accurate determination of MC-LR and can be referred to detect other hazardous substances in water products.
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http://dx.doi.org/10.1016/j.foodchem.2018.11.071DOI Listing
April 2019

A bimodal (SERS and colorimetric) aptasensor for the detection of Pseudomonas aeruginosa.

Mikrochim Acta 2018 10 31;185(11):528. Epub 2018 Oct 31.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.

An aptamer based assay is described for the determination of Pseudomonas aeruginosa (P. aeruginosa). It is bimodal in that both surface enhanced Raman spectroscopy (SERS) and colorimetry are applied for quantitation. The aptamer against P. aeruginosa and its corresponding complementary DNA fragment (cDNA) were conjugated to two kinds of differently sized gold nanoparticles (AuNPs). The 30 nm AuNPs carrying the aptamer are used as color signal probes, while the cDNA-15 nm AuNP conjugates serve as SERS signalling probes. In the absence of P. aeruginosa, the two probes assemble to form the duplex structure. When the probes are exposed to P. aeruginosa, the aptamer dissociates from its cDNA and binds the target. After centrifugation, the SERS signal from the supernatant decreased, due to the decreased electromagnetic effect. On addition of 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide, a relatively rapid catalytic reaction between horseradish peroxidase (linked to the aptamer on the 30-nm AuNPs) and TMB occurred, resulting in the formation of a green color with an absorption peak at 640 nm. The reliability of the approach was validated by detecting different levels of P. aeruginosa in spiked tap water and chicken meat samples. The average recoveries ranged from 88% to 112%, confirming the practicality of this method. In our perception, this dual mode aptasensor paves the way for accurate and reliable determination of P. aeruginosa. Conceivably, the method has a wide scope in that it may be extended to the determination of various other species for which respective aptamers are available. Graphical abstract Schematic illustration of a bimodal aptasensor based on surface enhanced Raman scattering (SERS) and color change for the detection of Pseudomonas aeruginosa.
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http://dx.doi.org/10.1007/s00604-018-3073-2DOI Listing
October 2018

A fluorometric assay for staphylococcal enterotoxin B by making use of platinum coated gold nanorods and of upconversion nanoparticles.

Mikrochim Acta 2018 10 25;185(11):516. Epub 2018 Oct 25.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China.

An aptamer based fluorometric assay is presented for fast and accurate detection of staphylococcal enterotoxin B (SEB). It is making use of platinum-coated gold nanorods ([email protected]) and upconversion nanoparticles (UCNPs). The aptamer against SEB is immobilized on [email protected] while the complementary DNA fragment of SEB aptamer is immobilized on UCNPs. As the concentration of SEB increases, the fluorescence of the satellite assembly ([email protected]) is gradually restored. Under the optimized conditions, fluorescence (best measured at excitation/emission wavelengths of 980/543 nm) linearly increases in the 2.0-400 pg·mL SEB concentration range. The limit of detection is as low as 0.9 pg·mL (at an S/N of 3), significantly lower than existing methods. The method was applied to the determination of SEB in spiked milk samples. The average recoveries ranged from 91.2% to 104.6%, confirming the practicality of this method. Graphical abstract Schematic illustration of a fluorometric assay based on inner filter effect (IFE) between platinum coated gold nanorods ([email protected]) and upconversion nanoparticles (UCNPs) for the determination of staphylococcal enterotoxin B (SEB).
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http://dx.doi.org/10.1007/s00604-018-3058-1DOI Listing
October 2018

Rapid detection of β-conglutin with a novel lateral flow aptasensor assisted by immunomagnetic enrichment and enzyme signal amplification.

Food Chem 2018 Dec 2;269:375-379. Epub 2018 Jul 2.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China. Electronic address:

A simple, rapid and economic lateral flow immunochromatographic assay (LFICA) was designed for ultrasensitive detection of β-conglutin. Instead of antibodies and gold nanoparticles (AuNPs) used in conventional LFICA, a cognate aptamer duo, binding to β-conglutin and [email protected] core-shell nanoparticles, was applied in this study. An enzyme signal amplification strategy was used to enhance sensitivity. In addition, a new magnetic enrichment strategy was employed to further enhance sensitivity of the assay, slowing down movement of the capture probe (i.e., [email protected] nanostructures) using an external magnetic field. The novel LFICA assay can be completed within 20 min and achieved a detection limit of 8 fM, a thousand-times lower than similar assays without magnetic focusing. Overall, our results demonstrated the potential for the proposed LFICA sensor in rapid detection of β-conglutin without any special analytical expertise or instrumentations.
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http://dx.doi.org/10.1016/j.foodchem.2018.07.011DOI Listing
December 2018

An ultrasensitive aptasensor based on fluorescent resonant energy transfer and exonuclease-assisted target recycling for patulin detection.

Food Chem 2018 May 3;249:136-142. Epub 2018 Jan 3.

Department of Agriculture & Biological Engineering, Bindley Bioscience Center, Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA. Electronic address:

Patulin (PAT) is a type of mycotoxin which can compromise both food quality and human health. Due to its harmful effects, strict monitoring of PAT contents in food systems is necessary. A novel kind of bioassay was proposed for determining PAT based on the fluorescent resonant energy transfer (FRET) strategy. The exonuclease-catalyzed target recycling strategy was employed to improve the sensitivity of the FRET system. The results showed that the linear range extends from 0.01 ng/mL to 100 ng/mL. Furthermore, the average recoveries ranged from 93.33% to 105.21%, confirming the reliability of this method. The total analysis time for our assay developed is about 50 min. Compared to traditional analytical methods, the developed assay is more stable and has a significantly lower detection of limit (0.003 ng/mL). We believe the approach developed in this study would be useful for high-throughput screening of PAT in food industry and government laboratory.
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http://dx.doi.org/10.1016/j.foodchem.2018.01.025DOI Listing
May 2018

Effect of exogenous metal ions and mechanical stress on rice processed in thermal-solid enzymatic reaction system related to further alcoholic fermentation efficiency.

Food Chem 2018 Feb 10;240:965-973. Epub 2017 Aug 10.

The State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address:

Metal-rich thermal-solid enzymatic processing of rice combined with yeast fermentation was investigated. 8 Metal ions were exogenously supplied at 0.05, 0.5 and 5mmol/100g (MG) rice prior to static high pressure enzymatic cooking (HPEC) and dynamic enzymatic extrusion cooking (EEC). Treated rice and its fermentation efficiency (FE) were characterized by rapid viscosity analyzer (RVA), UV-Vis, FT-IR and atomic absorption spectrophotometer (AAS). The optimum pH range of enzyme in solid system (>4.9) was broader than in a liquid system (>5.5). Cations decreased enzymatic activity in HPEC probably due to metal-induced aggregation of rice matrix with reduced reacting area as well as strengthened structure of starch/polysaccharides modified by metals. While using the EEC with mechanical mixing/shearing, relative activity was activated to 110 and 120% by Mg (0.05-0.5MG) and Ca (0.05-5MG), respectively. Furthermore, the effectiveness of residual ions to promote further FE was found to follow the order: Ca>K>Zn>Mg>Mn>Na≈Control>Fe>Cu, individually.
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http://dx.doi.org/10.1016/j.foodchem.2017.08.033DOI Listing
February 2018

Highly sensitive fluorescence sensing of zearalenone using a novel aptasensor based on upconverting nanoparticles.

Food Chem 2017 Sep 19;230:673-680. Epub 2017 Mar 19.

Department of Agriculture & Biological Engineering, Bindley Bioscience Center, Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA. Electronic address:

A facile strategy was successfully developed for the detection of zearalenone (ZEN). In this assay, highly fluorescent upconversion nanoparticles were synthesized and conjugated with the complementary oligonucleotide of ZEN aptamer for use as signal probes. Magnetic nanoparticles immobilized with the ZEN aptamer were assigned as capture probes. The results exhibited that the linear correlation between the decreased luminescence intensity of the signal and the concentration of ZEN was very strong (R=0.9957) in the range of 0.05-100μg/L. In addition, the limit of detection of the proposed method (0.126μg/kg for corn and 0.007μg/L for beer) was significantly lower than the existing methods. Furthermore, the reliability of the competitive immunoassay format was validated by comparing the results determined in real food samples to those obtained from a commercially available method. Overall, the novel aptasensor have showed great potential for rapid and accurate ZEN determination.
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http://dx.doi.org/10.1016/j.foodchem.2017.03.100DOI Listing
September 2017

Response surface methodology for evaluation and optimization of process parameter and antioxidant capacity of rice flour modified by enzymatic extrusion.

Food Chem 2016 Dec 28;212:146-54. Epub 2016 May 28.

The State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address:

For the purpose of investigating the effect of enzyme concentration (EC), barrel temperature (BT), moisture content (MC), and screw speed (SS) on processing parameters (product temperature, die pressure and special mechanical energy (SME)) and product responses (extent of gelatinization (GE), retention rate of total phenolic content (TPC-RR)), rice flour extruded with thermostable α-amylase was analyzed by response surface methodology. Stepwise regression models were computed to generate response surface and contour plots, revealing that both TPC-RR and GE increased as increasing MC while expressed different sensitivities to BT during enzymatic extrusion. Phenolics preservation was benefited from low SME. According to multiple-factor optimization, the conditions required to obtain the target SME (10kJ/kg), GE (100%) and TPC-RR (85%) were: EC=1.37‰, BT=93.01°C, MC=44.30%, and SS=171.66rpm, with the actual values (9.49kJ/kg, 99.96% and 87.10%, respectively) showing a good fit to the predicted values.
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http://dx.doi.org/10.1016/j.foodchem.2016.05.171DOI Listing
December 2016

Effect of chitosan molecular weight on the formation of chitosan-pullulanase soluble complexes and their application in the immobilization of pullulanase onto Fe3O4-κ-carrageenan nanoparticles.

Food Chem 2016 Jul 28;202:49-58. Epub 2016 Jan 28.

Key Lab of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, China.

The interactions between pullulanase and chitosans of different molecular weights (Mw) were comprehensively studied, and their applications in pullulanase immobilization onto Fe3O4-κ-carrageenan nanoparticles upon chitosan-pullulanase complexation were also evaluated. Chitosan (CS) complexation with pullulanase was found to be dependent on pH and chitosan Mw. The critical pH of structure-forming events during complexation shifted significantly (p<0.05) to a lower pH with a low Mw chitosan (50kDa) compared to other chitosan types. Binding constants for the chitosan-pullulanase interaction increased in the following order: CS-500
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http://dx.doi.org/10.1016/j.foodchem.2016.01.119DOI Listing
July 2016
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